RU2007142850A - DED PEPTID AND ITS APPLICATION FOR IDENTIFICATION AND / OR CLEANING OF RECOMBINANT PROTEINS, VECTOR PDED - Google Patents
DED PEPTID AND ITS APPLICATION FOR IDENTIFICATION AND / OR CLEANING OF RECOMBINANT PROTEINS, VECTOR PDED Download PDFInfo
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- RU2007142850A RU2007142850A RU2007142850/13A RU2007142850A RU2007142850A RU 2007142850 A RU2007142850 A RU 2007142850A RU 2007142850/13 A RU2007142850/13 A RU 2007142850/13A RU 2007142850 A RU2007142850 A RU 2007142850A RU 2007142850 A RU2007142850 A RU 2007142850A
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- identification
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- recombinant proteins
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Идентификационный пептид, характеризующийся аминокислотной последовательностью Gly-Pro-Ala-Pro-Gln-Pro-Asp-Glu-Asp-Leu-Lys-Arg-Gln. ! 2. Нуклеиновая кислота, характеризующаяся нуклеотидной последовательностью, кодирующей пептид по п.1. ! 3. Применение пептида по п.1 в качестве идентификационного пептида для идентификации и/или очистки рекомбинантных белков. ! 4. Применение по п.3, причем идентификационный пептид находится на С-конце или N-конце рекомбинантного белка. ! 5. Применение по п.3, причем идентификационный пептид находится в составе рекомбинантного белка в положении, отличном от С-конца или N-конца. ! 6. Применение по п.3, причем идентификация рекомбинантных белков осуществляется методом Вестерн-блоттинга. ! 7. Применение по п.3, причем идентификация рекомбинантных белков осуществляется иммуноцитохимическими методами. ! 8. Применение по п.3, причем при очистке рекомбинантных белков моноклональные антитела иммобилизованы на твердой фазе. ! 9. Вспомогательный вектор pDED для получения рекомбинантных ДНК для экспрессии интересующих рекомбинантных белков, содержащих пептид по п.1.1. An identification peptide characterized by the amino acid sequence Gly-Pro-Ala-Pro-Gln-Pro-Asp-Glu-Asp-Leu-Lys-Arg-Gln. ! 2. A nucleic acid characterized by a nucleotide sequence encoding a peptide according to claim 1. ! 3. The use of the peptide according to claim 1 as an identification peptide for the identification and / or purification of recombinant proteins. ! 4. The use according to claim 3, wherein the identification peptide is at the C-terminus or N-terminus of the recombinant protein. ! 5. The use according to claim 3, wherein the identification peptide is located in a recombinant protein at a position other than the C-terminus or N-terminus. ! 6. The use according to claim 3, wherein the identification of recombinant proteins is carried out by Western blotting. ! 7. The use according to claim 3, wherein the identification of recombinant proteins is carried out by immunocytochemical methods. ! 8. The use according to claim 3, and when purifying recombinant proteins, monoclonal antibodies are immobilized on the solid phase. ! 9. An auxiliary vector pDED for producing recombinant DNA for expression of interest recombinant proteins containing the peptide according to claim 1.
Claims (9)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2007142850/13A RU2380373C2 (en) | 2007-11-21 | 2007-11-21 | PEPTIDE DED AND ITS APPLICATION FOR IDENTIFICATION AND/OR PURIFICATION OF RECOMBINANT PROTEINS, VECTOR pDED |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2007142850/13A RU2380373C2 (en) | 2007-11-21 | 2007-11-21 | PEPTIDE DED AND ITS APPLICATION FOR IDENTIFICATION AND/OR PURIFICATION OF RECOMBINANT PROTEINS, VECTOR pDED |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2007142850A true RU2007142850A (en) | 2009-05-27 |
| RU2380373C2 RU2380373C2 (en) | 2010-01-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2007142850/13A RU2380373C2 (en) | 2007-11-21 | 2007-11-21 | PEPTIDE DED AND ITS APPLICATION FOR IDENTIFICATION AND/OR PURIFICATION OF RECOMBINANT PROTEINS, VECTOR pDED |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2380373C2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2493251C1 (en) * | 2012-07-16 | 2013-09-20 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Line of cells cho[v3d], secreting recombinant vessel endothelium growth factor (vegf) of humans, isoform a165, with exogenic 3×ded-epitope |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0827966T3 (en) * | 1996-09-09 | 2002-05-21 | Rmf Dictagene Sa | Preparation of purified (poly) peptides |
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2007
- 2007-11-21 RU RU2007142850/13A patent/RU2380373C2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2380373C2 (en) | 2010-01-27 |
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