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RU2004101340A - METHOD FOR PRODUCING A CATTLE TRANSFER FACTOR - Google Patents

METHOD FOR PRODUCING A CATTLE TRANSFER FACTOR Download PDF

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Publication number
RU2004101340A
RU2004101340A RU2004101340/15A RU2004101340A RU2004101340A RU 2004101340 A RU2004101340 A RU 2004101340A RU 2004101340/15 A RU2004101340/15 A RU 2004101340/15A RU 2004101340 A RU2004101340 A RU 2004101340A RU 2004101340 A RU2004101340 A RU 2004101340A
Authority
RU
Russia
Prior art keywords
transfer factor
hydrolysis
lymph nodes
solution
producing
Prior art date
Application number
RU2004101340/15A
Other languages
Russian (ru)
Other versions
RU2270021C2 (en
Inventor
Владимир Михайлович Щеглов (RU)
Владимир Михайлович Щеглов
Владимир Алексеевич Молоканов (RU)
Владимир Алексеевич Молоканов
пунов Сергей Анатольевич Л (RU)
Сергей Анатольевич Ляпунов
Елена Борисовна Табулович (RU)
Елена Борисовна Табулович
Original Assignee
ФГОУ ВПО Уральска государственна академи ветеринарной медицины (RU)
ФГОУ ВПО Уральская государственная академия ветеринарной медицины
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Application filed by ФГОУ ВПО Уральска государственна академи ветеринарной медицины (RU), ФГОУ ВПО Уральская государственная академия ветеринарной медицины filed Critical ФГОУ ВПО Уральска государственна академи ветеринарной медицины (RU)
Priority to RU2004101340/15A priority Critical patent/RU2270021C2/en
Publication of RU2004101340A publication Critical patent/RU2004101340A/en
Application granted granted Critical
Publication of RU2270021C2 publication Critical patent/RU2270021C2/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (4)

1. Способ получения трансфер-фактора, включающий получение экстракта лимфоцитов крови человека, с последующим гидролизом ферментом ДНК-азой, диализом и хромотографией, отличающийся тем, что, с целью применения трансфер-фактора в ветеринарной медицине, используют лимфатические узлы крупного рогатого скота, подвергнутые гомогенезации, гидролизу пепсином и консервации раствором фенола.1. A method of obtaining a transfer factor, including obtaining an extract of human blood lymphocytes, followed by hydrolysis with an enzyme DNA-ase, dialysis and chromatography, characterized in that, in order to use the transfer factor in veterinary medicine, lymph nodes of cattle are used, subjected to homogenization, hydrolysis with pepsin and preservation with phenol solution. 2. Трансфер-фактор по п.1, отличающийся тем, что для получения экстракта лимфоцитов в большем объеме используют лимфатические узлы.2. The transfer factor according to claim 1, characterized in that the lymph nodes are used to obtain a larger volume of lymphocyte extract. 3. Трансфер-фактор по п.1, отличающийся тем, что процесс гомогенизации лимфатических узлов заключается в их обработке в гомогенизаторе до однородной консистенции при 20 тысячах оборотов в минуту в течение 2-3 мин.3. The transfer factor according to claim 1, characterized in that the process of homogenization of the lymph nodes consists in processing them in a homogenizer to a homogeneous consistency at 20 thousand revolutions per minute for 2-3 minutes. 4. Трансфер-фактор по п.1, отличающийся тем, что для осуществления гидролиза экстракта лимфоцитов используют 0,5%-ный раствор пепсина на 0,5%-ном растворе соляной кислоты.4. The transfer factor according to claim 1, characterized in that for the hydrolysis of the lymphocyte extract using a 0.5% solution of pepsin in a 0.5% solution of hydrochloric acid.
RU2004101340/15A 2004-01-16 2004-01-16 Method for obtaining transfer-factor in cattle RU2270021C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2004101340/15A RU2270021C2 (en) 2004-01-16 2004-01-16 Method for obtaining transfer-factor in cattle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2004101340/15A RU2270021C2 (en) 2004-01-16 2004-01-16 Method for obtaining transfer-factor in cattle

Publications (2)

Publication Number Publication Date
RU2004101340A true RU2004101340A (en) 2005-06-20
RU2270021C2 RU2270021C2 (en) 2006-02-20

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Family Applications (1)

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RU2004101340/15A RU2270021C2 (en) 2004-01-16 2004-01-16 Method for obtaining transfer-factor in cattle

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2624504C1 (en) * 2015-12-30 2017-07-04 Маргарита Евгеньевна Дмитриева Method of obtaining transfer factor for preventing viral bird diseases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56500015A (en) * 1978-10-25 1981-01-08
SU787031A1 (en) * 1979-02-20 1980-12-15 Московский Научно-Исследовательский Институт Вакцин И Сывороток Им И.И.Мечникова Method of obtaining "transfer-factor" preparation

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RU2270021C2 (en) 2006-02-20

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20060117