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RU2002132049A - METHOD FOR PRODUCING LIPOPOLYSACCHARIDES - Google Patents

METHOD FOR PRODUCING LIPOPOLYSACCHARIDES

Info

Publication number
RU2002132049A
RU2002132049A RU2002132049/13A RU2002132049A RU2002132049A RU 2002132049 A RU2002132049 A RU 2002132049A RU 2002132049/13 A RU2002132049/13 A RU 2002132049/13A RU 2002132049 A RU2002132049 A RU 2002132049A RU 2002132049 A RU2002132049 A RU 2002132049A
Authority
RU
Russia
Prior art keywords
bacteria
lipopolysaccharides
producing
centrifugation
sedimenting
Prior art date
Application number
RU2002132049/13A
Other languages
Russian (ru)
Other versions
RU2237719C2 (en
Inventor
Геннадий Леонидович Бурыгин
Лариса Юрьевна Матора
Сергей Юрьевич Щеголев
Original Assignee
Институт биохимии и физиологии растений и микроорганизмов РАН
Filing date
Publication date
Application filed by Институт биохимии и физиологии растений и микроорганизмов РАН filed Critical Институт биохимии и физиологии растений и микроорганизмов РАН
Priority to RU2002132049/13A priority Critical patent/RU2237719C2/en
Priority claimed from RU2002132049/13A external-priority patent/RU2237719C2/en
Publication of RU2002132049A publication Critical patent/RU2002132049A/en
Application granted granted Critical
Publication of RU2237719C2 publication Critical patent/RU2237719C2/en

Links

Claims (1)

Способ получения липополисахаридов, включающий культивирование бактерий, осаждение их из среды культивирования центрифугированием, обработку протеолитическим ферментом протеиназа К до получения липополисахаридов, отличающийся тем, что после осаждения бактерий из их наружных мембран экстрагируют высокомолекулярный комплекс, состоящий из липополисахарида и белка путем добавления к бактериям раствора, содержащего ЭДТА в количестве 0,01-0,05 ммоль на 1 г влажных клеток, затем центрифугированием отделяют от бактерий полученный экстракт, который и подвергают обработке ферментом.A method of producing lipopolysaccharides, including culturing bacteria, sedimenting them from the culture medium by centrifugation, treating protease K with the proteolytic enzyme to obtain lipopolysaccharides, characterized in that after precipitating bacteria from their outer membranes, a high molecular weight complex consisting of lipopolysaccharide and protein is extracted by adding a solution to the bacteria, containing EDTA in an amount of 0.01-0.05 mmol per 1 g of wet cells, then the obtained extract is separated by centrifugation from bacteria, and subjected to enzyme treatment.
RU2002132049/13A 2002-11-28 2002-11-28 Method for preparing lipopolysaccharides RU2237719C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2002132049/13A RU2237719C2 (en) 2002-11-28 2002-11-28 Method for preparing lipopolysaccharides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2002132049/13A RU2237719C2 (en) 2002-11-28 2002-11-28 Method for preparing lipopolysaccharides

Publications (2)

Publication Number Publication Date
RU2002132049A true RU2002132049A (en) 2004-05-20
RU2237719C2 RU2237719C2 (en) 2004-10-10

Family

ID=33537300

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2002132049/13A RU2237719C2 (en) 2002-11-28 2002-11-28 Method for preparing lipopolysaccharides

Country Status (1)

Country Link
RU (1) RU2237719C2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2483112C1 (en) * 2012-04-03 2013-05-27 Федеральное казенное учреждение здравоохранения "Российский научно-исследовательский противочумный институт "Микроб" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека ("РосНИПЧИ "Микроб" Роспотребнадзора) Method to produce lypopolysaccharide of plague agent
RU2593946C1 (en) * 2015-04-13 2016-08-10 Государственное бюджетное образовательное учреждение высшего профессионального образования "Астраханский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ГБОУ ВПО Астраханский ГМУ Минздрава России) METHOD OF EXTRACTING LIPOPOLYSACCHARIDE Chlamydia trachomatis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2051969C1 (en) * 1992-07-31 1996-01-10 Иркутский научно-исследовательский противочумный институт Сибири и Дальнего Востока Method of bacterial lipopolysaccharide preparing
EP1154789A2 (en) * 1999-02-22 2001-11-21 University of British Columbia Caulobacter lps immunoadjuvant

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