RU126137U1 - CELL OF AN OPTICAL BULK CEVET OF A PHOTOMETRIC ANALYZER - Google Patents

Abstract

The cell of the optical bulk cell of the photometric analyzer, characterized in that it further comprises a removable adapter having a through channel, on the inner surface of which there are longitudinal grooves for installing an optical disposable capillary micro cell, the outer surface of the adapter is provided with guides for installing it in the cell of the optical bulk cell The adapter has slots for the passage of the light beam.

Description

The inventive device relates to the field of medicine and biology and can be used to determine the quantitative content of the analyte in physiological fluid. More precisely, the utility model relates to the nodes of devices for determining the quantitative content of the analyte in physiological fluid by optical methods, for example, spectrophotometric method.

Digital spectrophotometers are known, for example, PD-303S, manufactured by Apel Co., Ltd, Japan, intended for a wide range of studies. Test tubes, semi-microcuvettes or square cuvettes can be used as test containers in the spectrophotometer. The disadvantage of the spectrophotometer is the inability to use an optical disposable capillary microcuvette as a test capacity.

Blood analyzers are also known, for example, a Hemo Control photometer manufactured by EKF - diagnostic sales GmbH (Germany), designed to determine the concentration of hemoglobin in whole blood. It uses an optical disposable capillary microcuvette as a test container. However, the disadvantage of the described device is the inability to use a standardized square cuvette as a test container.

Closest to the claimed device in technical essence and the achieved result is the invention described in copyright certificate No. SU 1798662 "Device for photometric measurements using a spectrophotometer" and selected as a prototype. From the description it follows that the device for conducting photometric measurements using a spectrophotometer contains a cuvette compartment of a spectrophotometer with an optical system. The optical system includes a source and a receiver located on one optical axis parallel to the plane of the base of the spectrophotometer. In order to improve the usability of the device during serial changes with the vertical orientation of the radiation beam, the device is equipped with an additional unit mounted on the cuvette compartment and made of opaque material. Inside the block is a microplate with holes for the test fluid. The microplate is made with the possibility of its movement in the longitudinal and transverse directions. Also inside the block is a bracket with a fixing head and mirrors mounted on it to form a radiation beam whose axis is perpendicular to the optical axis on which the source and receiver are located, and the mirrors are optically coupled to the source and receiver. The axis of the fixing head is perpendicular to the optical axis on which the source and receiver are located. The unit is installed in the cuvette compartment of a standard spectrophotometer. The device described in the author's certificate allows measurements to be made both with standardized square cuvettes and with disposable capillary microcuvettes.

The disadvantages of this invention are both the difficulty in mounting it on a spectrophotometer and the large volume of the unit, which is installed in the cuvette compartment of a standard spectrophotometer.

The technical result, which is achieved in the claimed utility model, is to simplify the design by creating a universal cell of the optical bulk cell of the photometric analyzer, which allows you to install a disposable capillary microcuvette or optical bulk cell in the analyzer. Also, the technical result is to reduce the service time of the analyzing device during the transition from a disposable capillary microcuvette to an optical filling cuvette, since it does not require readjustment of the analyzing device.

The technical result is achieved by the fact that the cell of the optical bulk cell of the photometric analyzer further comprises a removable adapter. The adapter has a through channel, on the inner surface of which there are longitudinal grooves for installing an optical disposable capillary microcuvette. The outer surface of the adapter is provided with guides for installing it in the cell of the optical bulk cell. In this case, the adapter has slots for the passage of the light beam.

In the following, the claimed technical solution is illustrated by a detailed description of a specific but not limiting present solution, an example of its implementation and the accompanying drawings, in which:

figure 1 - cell in section;

figure 2 - cell with an installed optical loading cell in the context;

figure 3 is a cell with an adapter and an optical disposable capillary microcuvette in section;

4 is an example of a specific implementation of the adapter;

5 is an example of a specific implementation of the cell;

6 - installation of the adapter in the cell.

The photometric analyzer (figure 1) contains a housing 1 in which a cell 2 is installed. Cell 2 is provided with holes 3 for the passage of a light beam. A specific example of the implementation of cell 2 is shown in Fig.5. The internal cavity of the cell 2 in the cross section has the shape of a square and is designed to install an optical loading cell 4, as shown in figure 2.

The adapter 5, a specific example of an embodiment of which is shown in FIG. 4, is provided with guides 6 for mounting it in the cell 2. The installation of the adapter 5 in the cell 2 is shown in FIG. 6. The adapter 5 has a through channel 7, on the inner surface of which there are longitudinal grooves 8 for installing an optical disposable capillary microcuvette 9. Moreover, the adapter has slots 10 for the passage of the light beam.

The device operates as follows.

When using an optical loading cell 4 with a solution of lysed blood, the photometric analyzer provides the determination of the following blood parameters:

- concentration of total hemoglobin ctH;

- Fetal hemoglobin FHbF fraction;

- fraction of carboxyhemoglobin FCOHb;

- methemolobin fraction FMetHb;

To conduct these studies, an optical loading cuvette 4 is inserted into the cell 2, as shown in Fig.2. Set the mode of operation of the analyzer "cuvette" including the corresponding button. The analyzer is turned on by pressing the “on” button.

During the measurement process, the photometric analyzer determines the optical density of physiological fluid at all wavelengths λi, the working spectral range of the spectrophotometer.

Next, the multiplication obtained by measuring the optical densities D1 (λi) by the coefficients K1 (λi) corresponding to the optical filling cell 4. These coefficients must be pre-recorded in the analyzer memory. As a result, the optical density spectrum of the test substance is determined: D (λi) = D1 (λi) * K1 (λi) and the associated absorption spectrum A (λi).

When using a disposable capillary microcuvette 9 with a solution of lysed blood, the analyzer measures the following blood parameters:

- concentration of total hemoglobin ctHb;

- fraction of oxyhemoglobin FO2Hb;

- fraction deoxyhemoglobin FHHb;

- fraction of carboxyhemoglobin FCOHb;

- methemoglobin fraction FMetHb.

To conduct these studies, adapter 5 is installed in cell 2, as shown in FIG. 6. After installing the adapter 5 in its longitudinal grooves 8 install a disposable capillary microcuvette 9 with a solution of lysed blood.

Set the operation mode of the microcuvette analyzer, including the corresponding button. The analyzer is turned on by pressing the “on” button.

During the measurement process, the analyzer determines the value of the optical density of physiological fluid at all wavelengths λi, the working spectral range of the spectrophotometer (absorption spectrum).

Next, the multiplication obtained by measuring the optical densities D2 (λi) by the coefficients K2 (λi) corresponding to the optical loading cell 4. These coefficients must be pre-recorded in the analyzer memory. As a result, the optical density spectrum of the test substance is determined: D (λi) = D2 (λi) * K2 (λi) and the associated absorption spectrum A (λi).

Using the inventive device in a photometric analyzer allows photometric determination of the concentration of hemoglobin, various derivatives of hemoglobin and other substances without dosing of blood and plasma and without preparation of reagents, it is possible to carry out measurements using disposable capillary microcuvettes 9, and using traditional optical filling cuvettes 4 In addition, the claimed device allows to reduce the dimensions and simplify the design of the photometric analyzer, which ovyshaet reliability and efficiency of its work as a whole. The device can be widely used in medical institutions and research centers.

Claims (1)

The cell of the optical bulk cell of the photometric analyzer, characterized in that it further comprises a removable adapter having a through channel, on the inner surface of which there are longitudinal grooves for installing an optical disposable capillary micro cell, the outer surface of the adapter is provided with guides for installing it in the cell of the optical bulk cell the adapter has slots for the passage of the light beam.

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