RO137457A0 - Auxiliary material based on ca, s and n for soil fertilization for cultivation of cereals and other plants - Google Patents

Abstract

The invention relates to an auxiliary material based on Ca, S and N, which results as a by-product from the technological process of preparing collagen-based biostimulators and biofertilizers, the material being used in the field of agriculture for the complementary treatment of poor soils for the cultivation of cereals or other types of plants. According to the invention, the auxiliary material is obtained as a by-product in the process of preparing collagen-based fertilizers, by hydrolysis with calcium hydroxide, in the neutralization step with sulphuric acid, resulting in a paste with a concentration of at least 50% dry matter, containing minimum 40% Ca and S and minimum 6% protein relative to the amount of dry matter, and which can be dried in the form of granules with minimum 80% dry matter, the thus obtained material exhibiting biostimulation properties for the growth of corn plants and other plants, when dosed as a paste or granules in an amount of 2...20 t/ha.

Description

The invention presents an auxiliary material based on Ca, S and N, which results as a secondary product from the technological process of preparing biostimulators and biofertilizers based on collagen, and is intended for agriculture, for the complementary treatment of the soil, in the culture of cereals or other plant types.

The application of the product presented in the patent, when fertilizing soils poor in calcium, sulfur or nitrogen salts in the case of plant crops, represents an efficient way of recirculating some by-products, which would otherwise constitute waste, thus meeting the requirements regarding the circular economy, reducing energy consumption in the production of fertilizers and the reduction of costs for food production.

It is known that soils poor in calcium lead to deficiencies in plant physiology, the role of calcium being complex, balancing the pH, associating with minerals containing micronutrients, which are released over time [1], acting as a long-term stimulant long, to maintain fruit firmness or increase biomass [2]. Also, the presence of microorganisms (eg Thiobacillus sp. gram-negative bacteria) in the soil generates mechanisms for the solubilization of insoluble salts (calcium phosphate), by releasing organic acids with the capacity to chelate metals. Other types of soil microorganisms, nitrifiers, generate nitric acid in the first phase and lead to the solubilization of calcium [1], Calcium is an essential element in the structure of the cell wall of plants and has an important role in the absorption of other nutrients, influencing the ability of plants to adapts to thermal stress. Also, calcium has a role in counteracting the effects of alkaline salts and organic acids from plants [3].

Acidic soils generate deficiencies in plant growth and production, affect the activity, negatively influence the climate, lead to microbial populations, the availability of nutrients and the activity of pathogens on plants. Changes in the solubilization and leaching of calcium from the soil in case of heavy rainfall, or its accumulation in drought conditions [4].

The patent ΟΝ1011Θ6528Α [5] presents a liquid product with a composition of 0 g/L calcium in the form of calcium nitrate and other components such as: 3-15% ee and calcium lignosulfonate, 1-5% citric acid and 0 ,5-6% amino acids. Application 1

K O R T A N spol. s.r.o.

Jizni 210

463 34 Hrâdek nad Nisou DlC CZ40231143

RO 137457 The foliar AO of the product reduces the incidence of diseases caused by calcium deficiencies, thus the fruits (apples, cherries) are firmer and the number of degraded fruits is reduced.

Another patent, US 8,444,742 B2 [3], describes a formulation based on calcium (calcium hypophosphite, Ca(H2PO2)2 and/or calcium chelated with EDTA), with the addition of 8% nitrogen compounds, phosphorus , potassium, growth stimulants (acetaminophen, caffeine, anthranilic acid), 5-10% vitamins (ascorbic acid, biotin, d-biotin, pyridoxine-HCl, thiamine chloride, thiamine monohydrate, riboflavin, folic acid, niacin, niacinamide , pantothenic acid, pyridoxine chloride), 3% amino acids (0.025%-0.075% w/w L-tryptophan, 0.04-0.06% w/w hydroxyproline, proline, serine, etc.), carbohydrates, polysaccharides, boron sodium, 0.03-0.06% w/w glycolic acid and auxiliaries (3-10% non-alcoholic phosphate esters). The liquid formulation contains 2% calcium and 0.2% boron, hydroxyproline, proline and tryptophan. The effects that the formulation has on the plants are not specified.

US patent 10,737,987 B2 [6] presents a mixture consisting of 40-20 parts of nitric and phosphoric acids, 30%-35% urea, 40-45% ammonium nitrate, trace elements (Fe, Cu, Zn, Mn, Mg , Mo, S, Ni, B), dung or other excrement, ammonium nitrate and calcium or their combinations and calcium in slightly soluble or insoluble compounds (calcium nitrate, calcium chloride, calcium cyanamide, hydrated calcium sulfate, calcium carbonate), which is applied to the soil by spraying or dripping, at a pH between 7.8-7.9. It is mentioned that the mixture is a soil amendment, a stabilized source of carbon and nitrogen, in which the smell of excrement is reduced, frost resistance is ensured and it is not corrosive for distribution vessels. Calcium ensures the stabilization of heavy metals in the soil, necessary for plants, which would otherwise leach out. The mixture has been proven to ensure the health of the microbe population in the soil and is beneficial for the corn crop, recording a 13% increase in corn production and a 33% increase in sugar content.

Unlike the products described in the literature and in the cited patents, the material in this patent has the following advantages:

(S ^ ^is a secondary product that results during the preparation process of a foliar feminizer with biostimulating properties, based on collagen hydrolysates with \ '.X C... P//

KORTAN spol. s.r.o.

Jizni 210 463 34 Hrădek nad Nisou □IC CZ40231143

RO 137457 AO low molecular weight and is obtained in the final phase, when the calcium hydroxide used as a hydrolysis agent is neutralized with sulfuric acid. So it is a product that can be obtained simply, without high consumption of energy and chemical materials. - being a secondary product from the production of collagen hydrolysates, it is impregnated with protein hydrolyzate, with a stimulating role for the microbial flora of the soil and nutrient for the plant.

- it is a concentrated product, in the form of a paste with a minimum of 50% dry substance, which can also be formulated in the form of granules by drying, rich in mineral substances, available for the soil and for the plant, with calcium and sulfur content (minimum 40 %) and a content of protein hydrolysates of at least 6% (% relative to the dry substance).

- protein hydrolysates have a very low molecular weight (below 3kDa), with a minimum of 20% proteins with a molecular weight below 600 Da, which ensures a quick availability of organic nitrogen, but also of amino acids, hormonal precursors that have a bio-stimulating effect both for the microbiota soil as well as for plants.

- the product can be used as such, in which case it is very cheap, in the form of pellets or as a component in fertilizer formulations.

The experiments carried out to verify the stimulating properties for the corn crop took place in the case of the product in the form of granules, after drying the product, at the laboratory level and in the experimental field by distributing the product in the form of a paste.

Next, we present the auxiliary material based on calcium, sulfur and nitrogen for fertilizing the soil in the culture of cereals and other plants and two examples of application.

Example 1: The auxiliary material based on Ca, S and N resulted from the hydrolysis process of collagen membrane waste for the food industry, with calcium hydroxide, followed by neutralization with sulfuric acid, is in the form of a paste containing dry matter of at least 50%, calcium at least 20%, sulfur at least 20% and protein of at least 6% (percentages related to dry matter) (Table 1), or in the form of granules with 22% moisture, which have ^soil fertilization properties , correction of pH values, calcium and sulfur intake for plants.

< > J T A M spol. s.r.o.

Jizni 210

463 34 Hrâdek nad Nisou OlC CZ4023H43

RO 137457 AO

Example 2: Auxiliary material based on Ca, S and N, in the form of granules with 88% dry matter, which is used to treat the soil for the cultivation of corn or other plants, by dosing in concentrations of 1.5 t/ha, 3 t/ha or 6 t/ha, has the effect of stimulating plant metabolism by increasing the chlorophyll index (Figures 1Asi B), compared to the control treated with water, by 75% in the first stage of vegetation and by 36.4%, in a a the second stage of vegetation. The stimulating effect of the auxiliary material based on Ca, S and N on plant growth was recorded for the doses of 3 t/ha and 6 t/ha by increasing the height of the treated plants by 6.38.8% (Fig.2) and the thickness strains by 4.87% (Fig 3).

Example 3: Auxiliary material based on Ca, S and N, in the form of a paste, with 62% dry matter, which is used to treat the soil for the cultivation of corn or other plants, by dosing in concentrations of 15 t/ha, has effect: stimulation of plant metabolism, more uniform development of plants compared to current production, by increasing the chlorophyll index by 26.7% (Figure 4), increasing plant height by 11% (Figure 5), and the number of cobs by 2.2 %, compared to the control treated with water (Figure 6).

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K O R T A N spol. s.r.o.

Jizni 210 463 34 Hrâdek nad Nisou DlC CZ40231143

RO 137457 AO

Appendix

BIBLIOGRAPHY

1. A. K. Srivastava, Ethel Ngullie, Integrated Nutrient Management: Theory and Practice. Dynamic Soil, Dynamic Plant ©2009 Global Science Books.

2. A. K. Srivastava and Shyam Singh, L. G. Albrigo, Diagnosis and Remediation of Nutrient Constraints in Citrus, Horticultural Reviews, Volume 34. Edited by Jules Janick, 2008 John Wiley & Sons, Inc, p.36.

3. US 8,444,742 B2, Soluble Calcium Fertilizer Formulation, 2013..

4. M. M iran sari, Soil microbes and plant fertilization, Appl Microbiol Biotechnol (2011) 92:875-885.

5. CN101186528A, Liquid calcium fertilizer and preparation method thereof, 2013.

6. US 10,737,987 B2, Fluid ionized Compositions, Methods of Preparation and Uses Thereof, 2020.

Table 1 Chemical characteristics for the auxiliary material based on Ca, S and N in the form of paste

Characteristics stock Dry substance, % 62 Protein, %* 8 Ash, % 67.9 Like, %* 25.2 S, %* 20.3 Mg, %* 0.3

*% related to dry matter

Claims (2)

1. Auxiliary material based on Ca, S and N, characterized by the fact that it is obtained as a by-product in the preparation process of collagen-based fertilizers, by hydrolysis with calcium hydroxide, in the neutralization stage with sulfuric acid, when it results a paste with a concentration of at least 50% dry matter, with a content of at least 40% calcium and sulfur and at least 6% protein (% relative to dry matter), and which can be dried in the form of granules with at least 80% dry matter, which it has fertilizing properties for the soil intended for agricultural crops. 2. Auxiliary material based on Ca, S and N, according to claim 1, characterized in that it has properties of biostimulation of the growth of corn plants and other plants, in the case of dosing in the form of paste or granules, in a proportion of 2 -20 t/ha.