[go: up one dir, main page]

PT1318198E - Processo para produzir um polipeptido recombinante que envolve a adicao de um inibidor de quimotripsina ao meio de cultura celular - Google Patents

Processo para produzir um polipeptido recombinante que envolve a adicao de um inibidor de quimotripsina ao meio de cultura celular

Info

Publication number
PT1318198E
PT1318198E PT03000391T PT03000391T PT1318198E PT 1318198 E PT1318198 E PT 1318198E PT 03000391 T PT03000391 T PT 03000391T PT 03000391 T PT03000391 T PT 03000391T PT 1318198 E PT1318198 E PT 1318198E
Authority
PT
Portugal
Prior art keywords
inhibitor
cell culture
quimotrypsin
producing
culture medium
Prior art date
Application number
PT03000391T
Other languages
English (en)
Inventor
Adamson Lars
Walum Erik
Dixelius Johan
Lima Lie Kristina
Original Assignee
Biovitrum Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE9601855A external-priority patent/SE9601855D0/xx
Application filed by Biovitrum Ab filed Critical Biovitrum Ab
Publication of PT1318198E publication Critical patent/PT1318198E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
PT03000391T 1996-05-14 1997-05-13 Processo para produzir um polipeptido recombinante que envolve a adicao de um inibidor de quimotripsina ao meio de cultura celular PT1318198E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9601855A SE9601855D0 (sv) 1996-05-14 1996-05-14 Process for producing a protein
US1887496P 1996-05-29 1996-05-29

Publications (1)

Publication Number Publication Date
PT1318198E true PT1318198E (pt) 2006-08-31

Family

ID=26662616

Family Applications (2)

Application Number Title Priority Date Filing Date
PT03000391T PT1318198E (pt) 1996-05-14 1997-05-13 Processo para produzir um polipeptido recombinante que envolve a adicao de um inibidor de quimotripsina ao meio de cultura celular
PT97923382T PT934424E (pt) 1996-05-14 1997-05-13 Processo para produzir um polipeptido recombinante envolvendo a adição de um inibidor de proteases dependentes de metais ou ou quimotripsinas ao meio de cultura de células

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT97923382T PT934424E (pt) 1996-05-14 1997-05-13 Processo para produzir um polipeptido recombinante envolvendo a adição de um inibidor de proteases dependentes de metais ou ou quimotripsinas ao meio de cultura de células

Country Status (13)

Country Link
US (1) US5851800A (pt)
EP (1) EP0934424B1 (pt)
JP (2) JP2000509994A (pt)
AT (2) ATE321141T1 (pt)
AU (1) AU716245B2 (pt)
CA (1) CA2253287C (pt)
DE (2) DE69735510T2 (pt)
DK (1) DK0934424T3 (pt)
ES (2) ES2260524T3 (pt)
NO (2) NO325358B1 (pt)
NZ (1) NZ332751A (pt)
PT (2) PT1318198E (pt)
WO (1) WO1997043436A1 (pt)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
EP2130554B1 (en) 1999-02-22 2012-09-05 University of Connecticut Albumin-free factor VIII formulations
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
AU780810B2 (en) * 1999-08-05 2005-04-21 Baxalta GmbH Recombinant stable cell clone, its production and use thereof
WO2001052891A1 (fr) * 2000-01-20 2001-07-26 Nippon Organon K. K. Inhibiteurs d'invasion plasmodiale dans des erythrocytes
EP1520008B1 (en) * 2002-07-09 2012-09-05 Baxter International Inc. Animal protein free media for cultivation of cells
DK1576182T4 (da) * 2002-12-23 2020-04-20 Bristol Myers Squibb Co Produktkvalitetsforbedring ved fremgangsmåde med dyrkning af pattedyreceller til proteinfremstilling
AU2003300276B2 (en) * 2002-12-23 2010-07-01 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
EP1620457A2 (en) * 2003-04-25 2006-02-01 Immunex Corporation Inducers of recombinant protein expression
US7255288B2 (en) * 2004-03-08 2007-08-14 Wan Shan Chan Aroma therapy for fountain
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
EP1707634A1 (en) * 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
PT1974014T (pt) 2006-01-04 2017-05-26 Baxalta Inc Meios de cultura celulares livres de oligopeptídeos
CA2679107C (en) * 2007-02-23 2018-01-16 Sk Chemicals Co., Ltd. Process for producing and purifying factor viii and its derivatives
EP1988101A1 (en) 2007-05-04 2008-11-05 Novo Nordisk A/S Improvement of factor VIII polypeptide titers in cell cultures
WO2008135498A2 (en) * 2007-05-04 2008-11-13 Novo Nordisk A/S Prevention of protein degradation in mammalian cell cultures
BRPI0921429B1 (pt) 2008-11-07 2022-07-12 Takeda Pharmaceutical Company Limited Formulação farmacêutica liofilizada estável, e, método para preparar um fator liofilizado estável
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
CN103154233A (zh) 2010-10-05 2013-06-12 诺沃—诺迪斯克保健股份有限公司 生产蛋白质的方法
EP2906683B1 (en) 2012-10-15 2017-05-31 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
KR102670157B1 (ko) 2015-10-28 2024-05-29 주식회사유한양행 이중 작용 단백질 및 이를 포함하는 약학적 조성물
KR102668200B1 (ko) 2015-10-28 2024-05-23 주식회사유한양행 지속형 fgf21 융합 단백질 및 이를 포함하는 약학적 조성물
EP3568149B1 (en) 2016-11-10 2025-03-12 Yuhan Corporation Pharmaceutical composition for preventing or treating hepatitis, hepatic fibrosis, and hepatic cirrhosis comprising fusion proteins
EP3612637A4 (en) 2017-04-21 2021-01-27 Yuhan Corporation PROCESS FOR THE PRODUCTION OF DUAL FUNCTION PROTEINS AND THEIR DERIVATIVES

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5149787A (en) * 1984-05-22 1992-09-22 The Blood Center Research Foundation Method for maintaining intact, non-degraded factor VIII/von-Willebrand factor during blood processing
SE8501050D0 (sv) * 1985-03-05 1985-03-05 Kabivitrum Ab Biologically active fragments of human antihemophilic factor and method for preparation thereof
US5279939A (en) * 1986-08-18 1994-01-18 Smithkline Beecham Corporation Protein protease inhibitors from streptomyces
US4891356A (en) * 1987-07-15 1990-01-02 Brigham & Women's Hospital Proteinase inhibitors for treatment of gastrointestinal ulcer disease
JPH0387173A (ja) * 1987-09-10 1991-04-11 Teijin Ltd ヒト活性化天然型ファクター８ｃの製造方法及びそれに用いる形質転換体
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells
JP2769170B2 (ja) * 1987-12-08 1998-06-25 ザイモジェネティクス，インコーポレイティド 真核細肪中での同時発現
DK457788D0 (da) * 1988-08-16 1988-08-16 Nordisk Gentofte Fremgangsmaade til fremstilling af et oensket polypeptid
WO1990002175A1 (en) * 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
GB8827305D0 (en) * 1988-11-23 1988-12-29 British Bio Technology Compounds
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
SE465222C5 (sv) * 1989-12-15 1998-02-10 Pharmacia & Upjohn Ab Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning
AU7492291A (en) * 1990-02-26 1991-09-18 Board Of Trustees Of The Leland Stanford Junior University Identification and expression of insect steroid receptor dna sequences
US5189178A (en) * 1990-11-21 1993-02-23 Galardy Richard E Matrix metalloprotease inhibitors
US5304603A (en) * 1991-06-18 1994-04-19 The Population Council Leydig cell stimulator
US5661034A (en) * 1991-07-18 1997-08-26 Fuji Yakuhin Kogyo Kabushiki Kaisha Serum-free medium for tissue culture containing tissue inhibitor of metalloproteinases and method for growing cells using the medium
WO1993006217A1 (en) * 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES
CA2078721A1 (en) * 1991-09-24 1993-03-25 Hiroshi Yonemura Process for preparing human coagulation factor viii protein complex
US5278289A (en) * 1991-11-12 1994-01-11 Johnson Alan J Antihemophilic factor stabilization
US5296471A (en) * 1992-12-22 1994-03-22 Glycomed Incorporated Method for controlling o-desulfation of heparin and compositions produced thereby
AU7524994A (en) * 1993-08-12 1995-03-14 University Of Maryland Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof
US5631159A (en) * 1993-09-22 1997-05-20 Creative Biomolecules, Inc. Lipid-modified serum free media
US5543396A (en) * 1994-04-28 1996-08-06 Georgia Tech Research Corp. Proline phosphonate derivatives

Also Published As

Publication number Publication date
CA2253287C (en) 2008-02-19
ES2260524T3 (es) 2006-11-01
NO325633B1 (no) 2008-06-30
EP0934424A1 (en) 1999-08-11
ATE321141T1 (de) 2006-04-15
DE69735510T2 (de) 2006-08-24
NO325358B1 (no) 2008-04-07
DE69736391T2 (de) 2007-08-16
NO985297D0 (no) 1998-11-13
NO20065027L (no) 1999-01-14
NZ332751A (en) 2000-06-23
US5851800A (en) 1998-12-22
DE69736391D1 (de) 2006-09-07
ES2270460T3 (es) 2007-04-01
AU716245B2 (en) 2000-02-24
JP2000509994A (ja) 2000-08-08
ATE334223T1 (de) 2006-08-15
JP4896999B2 (ja) 2012-03-14
NO985297L (no) 1999-01-14
DK0934424T3 (da) 2006-11-20
WO1997043436A1 (en) 1997-11-20
AU2919797A (en) 1997-12-05
CA2253287A1 (en) 1997-11-20
EP0934424B1 (en) 2006-07-26
JP2009148271A (ja) 2009-07-09
PT934424E (pt) 2006-12-29
DE69735510D1 (de) 2006-05-11

Similar Documents

Publication Publication Date Title
PT1318198E (pt) Processo para produzir um polipeptido recombinante que envolve a adicao de um inibidor de quimotripsina ao meio de cultura celular
Ryan et al. Mammalian collagenase: direct demonstration in homogenates of involuting rat uterus
McKERROW et al. Proteinases from invasive larvae of the trematode parasite Schistosoma mansoni degrade connective-tissue and basement-membrane macromolecules
ATE140968T1 (de) Kultur des wachstumsfaktors der vaskularen endothelialzellen und dns, die dafür kodiert
ATE234108T1 (de) Therapeutikum zur unterdrückung von schnarchgeräuschen
AU576365B2 (en) Collagen and or fibrinogen as carrier of drugs for their introduction into cellular lesion areas
ATE110782T1 (de) Rekombinanter menschlicher endothelzellenwachstumsfaktor.
BR9910251A (pt) Estimulação hematopoiética
ES2166783T3 (es) Factor viii activado utilizado como agente terapeutico y metodo para el tratamiento de la deficiencia de factor viii.
ATE347585T1 (de) In-vitro wachstum von langerhans'schen inseln und ihre verwendungen
NO882635L (no) Fremgangsmaate for fremstilling av proteiner i aktive former.
ATE330613T1 (de) Verwendung von prostaglandin a oder dessen derivaten zur behandlung von psoriasis
HUP9901188A2 (hu) Új, meghatározott enzimkeverékek sejtkinyeréshez és sebkezeléshez
DE69020018D1 (de) Gentechnologische menschliche Cholinesterasen.
BR9506866A (pt) Composiçao biologicamente purificada composiçao para utilizaçao como um agente anti-inflamatório processo para o tratamento de mamíferos tendo tecido inflamado e processo para á produçao de ciclomarin-a
ES2099714T3 (es) Procedimiento de tratamiento endoproteolitico de las proteinas (precursores) y de produccion microbiologica de proteinas.
ES2043610T3 (es) Procedimiento para la produccion de un polipeptido hibrido del tipo activador de plasminogeno.
PE42695A1 (es) Procedimiento para producir proteina c
ES2056149T3 (es) Ambliommina, una nueva sustancia activa para la terapia anticoagulante.
PT883406E (pt) Utilizacao da il-7 no tratamento das doencas auto-imunes em particular a diabetes mellitus insulinodependente
DE3764559D1 (de) 4-benzyloxy-3-pyrrolin-2-on-1-yl-acetamid, dessen herstellung und verwendung.
WO1998041535A3 (en) Activation of peptides
KR890701733A (ko) 신규 인체 조직형 플리스미노겐 활성제 변이체
NZ516617A (en) Soluble, active recombinant human MAO-B
DE60044528D1 (de) Bazillus zelle für die herstellung von proteinen