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PH12016500782A1 - Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells - Google Patents

Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells

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Publication number
PH12016500782A1
PH12016500782A1 PH12016500782A PH12016500782A PH12016500782A1 PH 12016500782 A1 PH12016500782 A1 PH 12016500782A1 PH 12016500782 A PH12016500782 A PH 12016500782A PH 12016500782 A PH12016500782 A PH 12016500782A PH 12016500782 A1 PH12016500782 A1 PH 12016500782A1
Authority
PH
Philippines
Prior art keywords
cells
clustering
suspension
pluripotent stem
pancreatic endocrine
Prior art date
Application number
PH12016500782A
Inventor
Fryer Benjamin
Laniauskas Daina
Blackmoore Marcia
Wang Haiyun
Lilova Kostadinka
Nelson Shelley
Rosocha Elizabeth
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of PH12016500782A1 publication Critical patent/PH12016500782A1/en

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
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    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract

The disclosure provides methods of preparing aggregated pluripotent stem cell clusters for differentiation to endoderm progenitor cells, pancreatic endocrine cells, mesoderm cells or ectoderm cells. Specifically, the disclosure provides methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
PH12016500782A 2013-11-01 2016-04-27 Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells PH12016500782A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Publications (1)

Publication Number Publication Date
PH12016500782A1 true PH12016500782A1 (en) 2016-06-13

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PH12016500783A PH12016500783A1 (en) 2013-11-01 2016-04-27 Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells
PH12016500782A PH12016500782A1 (en) 2013-11-01 2016-04-27 Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells

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PH12016500783A PH12016500783A1 (en) 2013-11-01 2016-04-27 Suspension and clustering of human pluripotent stem cells for differentation into pancreatic endocrine cells

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EP (2) EP3063268A4 (en)
JP (2) JP2016534731A (en)
KR (4) KR20180128529A (en)
CN (2) CN105793413A (en)
AU (4) AU2014342995C1 (en)
BR (1) BR112016009393A8 (en)
CA (2) CA2928639A1 (en)
MX (2) MX2016005657A (en)
PH (2) PH12016500783A1 (en)
RU (2) RU2689710C2 (en)
SG (2) SG11201603045VA (en)
WO (2) WO2015065524A2 (en)

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JP2022547417A (en) * 2019-08-27 2022-11-14 ユナイテッド セラピューティクス コーポレイション Methods and compositions for culturing alveolar cells
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WO2022203051A1 (en) * 2021-03-25 2022-09-29 株式会社カネカ Method for producing pluripotent stem cell population
CN116218766A (en) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 A method for preparing pancreatic precursor cells, culture medium and application thereof

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SG11201603045VA (en) * 2013-11-01 2016-05-30 Janssen Biotech Inc Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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