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PE20010505A1 - A METHOD FOR THE PREPARATION OF DNA FROM BIOLOGICAL MATERIAL - Google Patents

A METHOD FOR THE PREPARATION OF DNA FROM BIOLOGICAL MATERIAL

Info

Publication number
PE20010505A1
PE20010505A1 PE2000000880A PE0008802000A PE20010505A1 PE 20010505 A1 PE20010505 A1 PE 20010505A1 PE 2000000880 A PE2000000880 A PE 2000000880A PE 0008802000 A PE0008802000 A PE 0008802000A PE 20010505 A1 PE20010505 A1 PE 20010505A1
Authority
PE
Peru
Prior art keywords
dna
preparation
biological material
resulting mixture
raw material
Prior art date
Application number
PE2000000880A
Other languages
Spanish (es)
Inventor
Terje Strom
Jens-Petter Jostensen
Per-Ivar Larsen
Original Assignee
Terje Strom
Jens-Petter Jostensen
Per-Ivar Larsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NO994144A external-priority patent/NO310114B1/en
Application filed by Terje Strom, Jens-Petter Jostensen, Per-Ivar Larsen filed Critical Terje Strom
Publication of PE20010505A1 publication Critical patent/PE20010505A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE REFIERE A UN METODO PARA LA PREPARACION DE ADN A PARTIR DE UN MATERIAL BIOLOGICO MEDIANTE LA EBULLICION DE LA MATERIA PRIMA EN PRESENCIA DE ALCALI Y SAL DISTRIBUIDOS UNIFORMEMENTE CON LA MATERIA PRIMA, LA MEZCLA RESULTANTE SE FILTRA, Y EL ADN SE VUELVE A CAPTURAR MEDIANTE PRECIPITACION DESPUES DE ACIDULAR EL PRODUCTO FILTRADO, LA MEZCLA RESULTANTE SE NEUTRALIZA ANTES DE LA PREPARACION DE ADNREFERS TO A METHOD FOR THE PREPARATION OF DNA FROM A BIOLOGICAL MATERIAL BY BOILING THE RAW MATERIAL IN THE PRESENCE OF ALKALI AND SALT UNIFORMLY DISTRIBUTED WITH THE RAW MATERIAL, THE RESULTING MIXTURE IS FILTERED, AND THE DNA IS RE-CAPTURED BY MEANS PRECIPITATION AFTER ACIDULATING THE FILTERED PRODUCT, THE RESULTING MIXTURE IS NEUTRALIZED BEFORE DNA PREPARATION

PE2000000880A 1999-08-26 2000-08-25 A METHOD FOR THE PREPARATION OF DNA FROM BIOLOGICAL MATERIAL PE20010505A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NO994144A NO310114B1 (en) 1999-07-19 1999-08-26 Method of Preparation of DNA

Publications (1)

Publication Number Publication Date
PE20010505A1 true PE20010505A1 (en) 2001-04-28

Family

ID=19903701

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2000000880A PE20010505A1 (en) 1999-08-26 2000-08-25 A METHOD FOR THE PREPARATION OF DNA FROM BIOLOGICAL MATERIAL

Country Status (5)

Country Link
EP (1) EP1206531A1 (en)
AU (1) AU6880600A (en)
IS (1) IS6277A (en)
PE (1) PE20010505A1 (en)
WO (1) WO2001014541A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104789554B (en) * 2015-04-10 2017-11-28 中国海洋大学 The method that DNA is prepared from waste liquid after the extraction of intestinal mucosa heparin
CN110437983A (en) * 2019-07-09 2019-11-12 南京格致医学检验有限公司 A DNA extraction device and method based on urine precipitation and boiling

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH529769A (en) * 1971-04-16 1972-10-31 Rephamac Ag Process for the preparation of high molecular weight deoxyribonucleic acids
DE3724442A1 (en) * 1987-07-23 1989-02-02 Europ Lab Molekularbiolog METHOD AND DEVICE FOR PURIFYING M-13 PHAGE DNA
MX9202037A (en) * 1991-05-03 1992-11-01 Becton Dickinson Co PURIFICATION BY DNA FILTRATION.

Also Published As

Publication number Publication date
EP1206531A1 (en) 2002-05-22
AU6880600A (en) 2001-03-19
WO2001014541A1 (en) 2001-03-01
IS6277A (en) 2002-02-21

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Legal Events

Date Code Title Description
FX Voluntary withdrawal