LU80067A1 - PROCESS FOR OBTAINING APPETITIVE HIGH POWER BAITS FOR SYNANTHROPIC FLY CONTROL - Google Patents

Description

r - '* "'? D. 4o.997 —so e-frf GRAND-DUCHÉ DE LUXEMBOURG Patent N ° ......................... ........................... 1 of l.ox ...... ao.û.t ..... l9. 7.3 .... 0] [p Mr. Minister. 0¾¾¾¾ of the National Economy and the Middle Classes

Title issued r ........................................

gWrgj Industrial Property Service

yM? LUXEMBOURG

Patent Application * * Requ I. Request

_______The company, ditet AIRwICK AG "at 4oo5 3SI-E" Suissef làcn · * (D

_ fcôo pag Mr. Jacques de Muyser, acting in general ·? 1 i, '~ Λ: lo ...................

..... agent ............................................ .................................................. .................................................. .................................................. ............................... (2) deposits ________ this first ..... August ISoo seventy-eight. ............................................ (3) to ____________ 1.5 ............ hours, at the Ministry of National Economy and the Middle Classes, in Luxembourg: 1. this request for obtaining a patent for invention concerning: ... .. "Process <31 obtaining ... d ^ app & ta ..... & .-. Ha» fc ..... peuto.lg_-apP'êtitif _ .. poar ...... (4) _____________la 1 ntte against synanthropic flies "________________________________________________________________________________ / 1 / / §é / qii / ^ é jii / é / khlüf ^ s / / eiii ..................... .................................................. .................................................. .........-........................................ ..................... .................................................. ................................. (5) 2. the delegation of power, dated ..... .. BASEL ............................................... on .., .. 1.3 ...... iui.ll..sfc ..... i.9.7 ..?.

3. description in language ............. IX.ailÇ.ais.8 ........................ ....... of the invention in two copies; 4 ............ U ............ drawing boards, in two copies; 5. the receipt of the fees paid to the Luxembourg Registration Office on ..... 1st ..... August ..... 19.7S .............. .................................................. .................................................. .................................................. ...............................................

claims for the above patent application the priority of one (s) request (s) of (6) ........................... .................................................. ................... filed in (7) ....................... .................................................. .................................................. ..........

the ................................................. .................................................. .................................................. .................................................. .................................................. ....................... (8) in the name of ..................... .................................................. ........................................ (9) elect domicile for him / her and, if appointed, for its representative, in Luxembourg .....................................

......... $ 3., ..... bld. "..... Royal ....................... .................................................. .................................................. .................................................. .............................. (io) requests the issue of a patent for the invention described and represented in the appendices t above, - with postponement of this issue to .............. 1.® .................... ..... months.

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V '·> ._- s., II. Deposit Minutes

The above application for a patent for invention has been filed with the Ministry of National Economy and the Middle Classes, Industrial Property Service in Luxembourg, on: August 1, 1978 .. ·· ........ ... ··. Pr. The Minister at ............. 15 ........ hours / \ of the National Economy and the Middle Classes, / \ p. d.

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fi \ Name. name. -firmp. adrpcçp / o \ c »it was going to link« rf »nr £ sÉ» r »iA nar *» »leaving é» ti nnaTît Hp mandptairp - / 31 rtntp H »i D. 40.997 *% Descriptive memory filed in support of 'a request of

PATENT

at

Luxembourg

formed by: airwick. AG

for: "Method of obtaining baits with high appetitive power for the fight against synanthropic flies".

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The present invention relates to compositions for use of bait, intended for the destruction of synanthropic flies, having a very high appetitive power for said flies and-characterized in that their odor is pleasant for humans.

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Synanthropic flies are annoying and / or harmful insects vis-à-vis humans, in particular by the germs of disease they carry (Trypanosomes, Rickettsiae, tuberculosis bacillus, leprosy bacillus, cholera vibrio, typhus bacillus, dysenteric bacillus, anthrax bacterium, diphtheria bacillus, amoebae, various protozoa and eruptive fever viruses) and the larvae of which can also cause intestinal or skin myases.

We understand why man seeks to destroy, everywhere in the world, insects of this category. Such are, in particular, the dipterans of the muscidae, sarcophagidae and tachinidae families.

Many means of destruction have been developed such as powders, liquids, aerosols and insecticide vapors but all these means are more or less dangerous for the user and, more generally, for his environment by the toxic materials which they spread on the surfaces and / or in the atmosphere.

Toxic baits generally do not have these drawbacks but, on the other hand, their effectiveness is linked to their level of attractiveness and appetite exerted on insects since it is necessary that they come to consume so that the toxic can to act.

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An excellent means of increasing attractiveness and appetite consists in introducing into the baits an amino acid vector such as a peptide, a protein or an arainoacid1

However, these amino acid vectors have their own odor and / or an odor from the start of decomposition which are unpleasant for humans and. can even be repulsive.

The addition of a substance capable of neutralizing these odors or flavoring agent is therefore highly desirable. But there are few such substances and those which possess this property are repellent to insects so that the bait becomes ineffective.

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effective.

However, it has now been found by the Applicant, that certain natural fruit juices, syrups and concentrates have the property of completely canceling the perception of odors emanating from the amino acid vectors without exerting the slightest repulsion on synanthropic flies. These substances are juices, syrups or natural extracts of raspberry or blackcurrant. L

The invention is therefore aimed at a composition for the use of bait comprising.

a food which is or which contains an amino acid vector, characterized in that it comprises a flavoring agent chosen from juices, syrups and natural concentrates of raspberry and / or Cassis.

This composition is preferably associated with an insecticidal agent which causes the destruction of the insects consuming it.

The flavoring agent may or may not contain one or more sugars added to those naturally contained in the fruit from which it comes.

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The fruit juices suitable within the meaning of the invention are all those obtained according to the known art, and using the various means of pressure of the fruits considered. with or without filtration. I

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The fruit concentrates suitable for the purposes of the invention are all those obtained according to the known art and which consist in particular in subjecting the juices of the fruits under consideration to a concentration under reduced pressure or to crystallization by cold. The fruit concentrates can optionally contain a part of alcohol, this being able to come, for example, from a deppectinization operation by precipitation.

Fruit syrups suitable for the purposes of the invention are all those obtained by the addition of a sugar to a fruit juice or a fruit extract, the latter> possibly being added with water. The fruit syrups are obtained by simple mixing of the constituents, with sugar dissolution with or without heating, this latter operation being able in particular to lead to syrups having the consistency of a jelly.

The concentration of flavoring in the bait is. preferably between 0.5 and 10% by weight and better still between about 1 and 8%, these percentages relating to the total weight of the bait composition.

The bait may also, optionally, contain an attractive and / or appetitive material chosen from those known to those skilled in the art, for example, natural or artificial flavors of cheese, meat and fruit, amino acids. , pollen extracts, thymol, skatole, indole, eu-genol, paraformaldehyde, hexamethylene tetramine, ammonium carbamate, aliphatic amines, papain, pancreatin, aliphatic acids , vanillin, 3-chloro-3-methyl-butene-1, 3-chloro-methyl-3-butene-2 and heavy alkenes comprising 20 to 27 carbon atoms such as (cis) -trico-sene-9.

The composition may also, optionally, contain a binding agent such as a natural or synthetic adhesive and / or a dye or pigment and / or a stabilizing agent.

The amino acid vector is chosen from the biochemical class of proteins, that is to say from the amino acids proper, peptides and proteins. 1 "· /" · · i - 4 - * "'>

Among the amino acids, there may be mentioned, for example, alanine, arginine, aspartic acid, cysteine, cystine, glutamic acid, glycine, histidine hydroxyproline, isoleucine , leucine, lysine, methioinine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine.

The peptides that can be used are natural or synthetic compounds, formed by the union of a relatively small number of amino acids. Among these, there may be mentioned, for example, glutathione, which is found in yeast and in the 1 intracellular components of mammalian tissues, carnosine, which is found in the striated muscles of mammals and fish, anserine, which is found in the striated muscles of birds, polyglutamic acid, which is found in microorganisms and the various polypeptide chains resulting from incomplete protein hydrolysis.

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Proteins are present in all the tissues of living animals and plants. They are formed by the union of a large number of amino acids. Among these, there may be mentioned, for example, zein, gliadin, hordein, ara-china and globulins, present in vegetable seeds, myosin, actin and tropomyosin, present in animal muscle fibers, seru-albumin, lipoproteins, hemoglobin and fibrinogen, present in the blood, ovalbumin, conalbumin, lisozyme and avidin, present in eggs, casein, and, lactoglobulin, present in milk, collagen and elastin, present in tendons and connective tissues, reticulin, present in the skin and adipose tissues, corneine, ichthyocol and elastin, present in fish tissue.

The proportion of amino acid vector in the food is between 2 and 100% by weight of the food, the rest, if any, consisting of a non-protein nutrient.

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Preferably, the proportion of amino acid vector in the food is at least equal to 10 7.

* The amino acid vector is used in the isolated state or in the raw state in the material constituting its preferably dehydrated original medium.

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As examples of unprocessed amino acids and peptides, there may be mentioned whole or partial hydrolysates of unprocessed proteins.

As examples of materials containing proteins in the raw state, and possibly peptides, the following may be cited, the values in parentheses representing the average percentage of proteins present: A. Dried fruits

Dried apricots (5.2), dried dates (2.2), dried figs (3.1), dried peaches (3.0), dried pears (2.3), dried apples (3.0), dried plums (2 , 3), raisins (2,3), almonds (18,6), peanuts (30,6), hazelnuts (12,7), nuts (15,0), cocoa (20,4).

B. Pulses

Dried cabbage (13.7), dried carrot (4.0), dry white bean (22.0), dried lentil (25.7), dried onion (10.1), dried peas (24.5), apple dried earth (7.1).

C. Cereals

Oat flakes (13.0), wheat flour (12.2), mat flour (9.2), rice flour (7.4), rye flour (11.0), soy flour ( 35.9), wheat germ (25.2), pearl barley (9.0), dried wheat bread (9.8).

D. Dairy products

Whole milk powder (25.8), skim milk powder (35.6), Emmental cheese (28.6), Limburg cheese (23.5), Parmesan cheese (36.3), Roquefort (21,7), powdered egg (48,2).

E. Meats

Bovine meal (65), bovine offal meal (72), horse meal (76),> rabbit meal (74), ovine meal (68), pig meal (69), flour cetaceans (78), poultry meal (73), poultry offal meal (75).

F. Fish and shellfish

Cod flour (63), tuna flour (82), shrimp flour (67), herring flour (70).

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G. Miscellaneous. Dry gelatin (85.6), dark chocolate (5.5), milk chocolate (6.0), molasses (2.4), pressed brewer's yeast (13.3), dry brewer's yeast (46.1 ), dry mushrooms (40).

The non-protein nutrient, when it exists, is chosen from carbohydrates such as, for example, sucrose, maltose, k lactose, glucose, fructose, galactose, raffinose, invert sugar, honey and starch and among lipids.

Said carbohydrates and lipids can be found naturally in materials containing the amino acid vector in the raw state, alongside various natural components such as celluloses, or on the contrary can be added to said materials in the composition.

As examples of carbohydrates that can be added to the composition, in addition to those previously mentioned, mention may be made of foods of plant origin which are poor in proteins such as Tapioca.

As examples of lipids which can be added to the composition, there may be indicated butter, vegetable oils, lard, tallow and fish oils.

The insecticidal agent when it is present in the compositions according to the invention can be chosen from all those acting by ingestion on insects: the preferred insecticidal agents are chosen from aromatic or heteroaromatic N-substituted carbamic esters and compounds organophosphates.

As examples of suitable carbamic esters, mention may be made of: - 3-methyl-5-pyrazolyl dimethylcarbamate, - 1-dimethylcarbamyl-5-methyl-5-pyrazolyl dimethylcarbamate (Dimetilan), - 2-isopropoxyphenyl methylcarbamate- 1 (Arprocarb), - 1-phenyl-3-methyl-pyrazolyl-5 (Pyrolan) dimethylcarbamate, - 7 - - 2-methyl-4-pyrimidyl-6-pyrimidyl-6 (Pyramat) dimethylcarbamate, - (1-dioxolane methylcarbamate) , 3 yl-2) -2 phenyl (dioxacarb), - 5-tetrahydro N-methylcarbamate; 6,7,8 naphthyl-2, - 2-methyl quinolyl-8 N-methylcarbamate, - N-methylcarbamate orthotertiobutylphenyl, - orthotertiobutoxyphenyl N-methylcarbamate, - ortho- (2-yloxy) phenyl N-methylcarbamate, - ortho-sec-butoxyphenyl N-methylcarbamate, - di-methylcarbamate N-methylcarbamate -2.6 phenyl, - 2,6-di-sec-butyl-phenyl N-methylcarbamate, - 2,6-diisopropyl-2,6-phenyl N-methylcarbamate, - 2-tert-butyl-6-methylphenyl N-methylcarbamate, - the di 5,5-dimethylmethylcarbamate-1-cyclohexene-one-3 yl (Dimetan),, - isopropylidene-2,3-dioxyphenyl N-methylcarbamate (Bendiocarb).

Among these carbamic esters, dimé-tilan and dioxacarb are particularly preferred.

As examples of suitable organophosphorus compounds, mention may be made of: - Ο, Ο-dimethyl and 0- (2,2-dichloro-vinyl) phosphate (Dichlorvos), - - Ο, Ο-dimethyl and 0- ( dibromo-1,2,2-dichloro-2,2 vinyl) (dibrone] - O, O-dimethyl and S- (aza-4-chloro-2-oxo-2-benzoxazolyl-2) methyl thiophosphate (azamethiphos), - (dimethoxy -thioxo-phosphoranylthio) -2 ethyl succinate (malathion), - Ο, Ο-diethyl and 0- (2-isopropyl-methyl-6-pyrimidyl-4) thiophosphate (diazinon), - O, O- thiophosphate dimethyl and O- (4-bromo-2,5-dichloro-phenyl) (bromophosethyl), - O thiophosphate, O-dimethyl and 0- (3-methyl-4-phenyl) (fenitrothion), - O thiophosphate, O-dimethyl and O- (2-methoxycarbonyl-propene-1 yl) (Metharzifos) - Ο, Ο-diethyl thiophosphate and 0- (3,5,6-trichloro-2-pyridyl), - 0, O thiophosphate -dimethyl and 0- (2,4,5-trichlorophenyl) (fenchlorphc: - 0, O-dimethyl and 0-methyl-3-nitro-phenyl thiophosphate) (fenitrothion - thiopho O, O-dimethyl and O- (2,5-dichloro-4-iodo-phenyl) sphate (iodfenphos), - dimethyl (2,2,2-hydroxy-1-trichloro) dimethyl phosphonate (trichlorphon).

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Among these organo-phosphor compounds #s, very particularly preferred are those whose vapor pressure at 20 ° C. is less than 0.01 Torr and in particular malathion, azamethiphos, fenitrothion and their binary and ternary mixtures.

In addition to carbamic esters and organophosphorus compounds, or in combination with these substances, other insecticidal materials can be used such as natural pyrethrins, rotenone, synthetic pyrethroids, N-N'-dibutyl parachlorobenzene-sulfonamide, 1 'octachloro-1,2,4,5,6,7, Ö, 8 tetrahydra-3a, 4,7,7a methano-4: 7 indane, the gamma stereoisomer of 1,2-hexa-chloro, 4,5,6 cyclohexane, endo-exo-hexachloro'-2,3,3,4,10,10 epoxy-6,7 octahydro-1,4,4a, 5,6,7,8,8a dimethano 1: 4.5: 8 naphthalene, sodium fluoride, boric acid and its salts.

The concentration of insecticide in the bait may vary. It is advantageously between approximately 0.01 and 8% by weight and preferably between 0.2 and 5%.

The compositions according to the invention are generally prepared by simple mixing of the constituents, however, for a better distribution of the insecticidal active ingredient and of the dye, it is preferable, in certain cases, to proceed as follows: a) mixture of nutritive constituents and flavoring; b) dissolving the insecticide active ingredient and the dye in a sufficiently volatile solvent such as acetone or methylene chloride; „C) spraying solution b on mixture a; d) total evaporation of the volatile solvent.

The optimal particle size of the composition varies according to the kind of insect whose destruction is targeted; in general, the grain size should be all the finer as the insect for which the bait is intended is smaller.

Preferably, this is between 0.05 mm and 1 mm.

The bait can be presented in the form of a loose powder or, on the contrary, in an agglomerated form such as granules or tablets with or without the aid of a binder.

- 9 - The bait can also be fixed on an absorbent support or absorbed therein, said inert support being able to consist, for example, of paper, blotter, cardboard, plastic material such as polystyrene, chloride of polyvinyl, polyvinyl acetate and cellulose acetate, pumice glass, crushed marble or silica or mineral silicates.

The advantage of the bait traps according to the invention is illustrated by the following experiments.

Experiment A *

The baits A-0, Al and A-2 not in accordance with the invention were prepared, and the baits A-3 and A-4, in accordance with the invention, constituted as follows (percentage by weight), the bait A-0 containing no flavoring agent and the others having part of the food material replaced by a flavoring agent.

A-0 A-l A-2 A-3 A-4

Sugar 20.2 19.0 19.0 19.0 19.0

Meat meal 63; 5 60.3 60.3 60.3 60.3

Vegetable protein hydrolyzate (a) 10.1 9.5 9.5 9.5 9.5

Dimétilan (b) 4 ^ 7 477 4/7 4/7 T / 7

Natural strawberry concentrate (c) - 5.0 - - -

Artificial blackcurrant flavor (c1) - - 5.0

Natural blackcurrant concentrate (d) - - - 5.0

Natural Raspberry Concentrate (5.0)

Red dye * 0.8 0.8 0.8 0.8 0.8

Potassium sorbate 0.5 0.5 0.5 0.5 0.5. (Cis) -tricosene-9 0.2 0.2 0.2 0.2 0.2 (a) Product derived from the hydrolysis of plant proteins containing short chain peptides and most of the amino acids present in . vegetable protein materials.

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Cb) Ν, Ν-dimethylcarbamyl-1-methyl-5-pyrazolyl-3-dimethylcarbamate.

(c) Reddish brown liquid originating from the concentration under reduced pressure of a natural strawberry juice added with 4% ethanol and marketed under the name natural strawberry flavor n ° 16643 by the French firm ROURE, BERTRAND and DUPONT.

(c1) Almost colorless oily liquid composed essentially of synthetic product and marketed under the name Cassis ne 144 H by the French firm MERO and BOYVEAU.

(d) Dark red liquid from the concentration under reduced pressure of a natural blackcurrant juice, partially depectinized by the addition * of 24% ethanol and marketed by the French firm ROURE, BERTRAND and DUPONT.

(d1) Brownish red liquid from the concentration under reduced pressure of a natural raspberry juice, partially depectinized by the addition of 17% ethanol and marketed by the French company ROURE, BERTRAND and DUPONT.

Two grams of each of these baits, reduced to homogeneous powder, were deposited, after drying, at the bottom of a rectangular box in black cardboard, obtained by cutting, folding and gluing; the base of the box measured 100 x 100 cm and the height was 5 cm; two elongated openings of 10 cm2 each were cut on the upper side.

The devices thus produced were placed in a 40 m3 room maintained at a temperature of 25 ° C and a relative humidity of 40; flies of the Musca domestica species were then released (approximately every 15 minutes, the number of dead flies in each device was counted for two hours. Five repetitions of this test were made with permutation of the devices each repetition.

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The overall results of all the tests are summarized in the table below (average numbers of dead flies counted per test).

Maturities in minutes À-0 Ä-1 A-2 A-3 A-4 '15 34.3 8.1 6.1 34.6 34.6 30 40.9 9.3 6.2 42.4 40, 4 45 44.9 13.9 7.4 44.6 '42.9 60 46.6 16.3 8.7 45.5 44.6 90 49.2 19.8 9.9 48.2 46.8 120 50.3 19.8 9.9 1 4?, 3. 47.2

It can be seen that the two baits conforming to A-3 and A-4 to the invention have an insecticide efficacy which is practically identical to that of the bait A-0 without flavoring agent, which is not the case when the flavoring agent added. does not conform to the definition of the invention as in the case of baits Al and A-2 although the latter have several attractive agents, such as the amino acid vectors, (cis) -tricosene-9 and a red dye.

Experiment B

The operation was carried out as in experiment A using the baits B-1 to B-5, containing a flavoring agent not in accordance with the invention, in comparison with a bait B-0 containing no flavoring agent (percentages by weight).

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v -1.2- λ * B-Ο Β-1 Β-2 Β-3 Β-4 Β-5

Sugar 20.2 ”19.0 1970 TÖTÖ 1970 1970

Meat meal 63.5 60.3 60.3 60.3 60.3 60.3

Vegetable protein hydrolyzate (a) 10.1 9.5 9.5 9.5 9.5 9.5

Dimetilan (b) 4.7 4.7 4.7 4.7 4.7 4.7

Artificial aroma of gooseberry - 5.0

Artificial currant flavor (e1) - - 5.0 -

Raspberry artificial flavor (e "), - 5.0

Raspberry artificial flavor (e "') - * - - 5.0

Artificial cherry flavor (e "") - - - - - 5.0

Red dye 0.8 0.8 0.8 0.8 0.8 0.8

Potassium sorbate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 (Cis) -tricosene-9 0.2 0.5 0.5 0.5 0.5 0.5 (e) Liquid slightly yellow oily composed essentially of synthetic products and marketed under the name of redcurrant composition No. 1244 D by the French firm MERO and BOYVEAU.

(e ') Oily almost colorless liquid composed essentially of synthetic products and marketed under the name of redcurrant composition No. 699 S by the French firm MERO and BOYVEAU.

(e ") Almost colorless oily liquid composed essentially of synthetic products and marketed under the name Composition raspberry n ° 1209D by the French firm MERO and BOYVEAU.

(e "') Oily almost colorless liquid composed essentially of synthetic * products and marketed under the name Composition raspberry n ° 1090 D by the French firm MERO and BOYVEAU.

(e "n) Slightly yellow oily liquid composed essentially of synthetic products and marketed under the name of Cherry Composition No. 09031 by the French firm MERO and BOYVEAU.

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The overall results of the tests are summarized in the table below (average numbers of dead flies counted per test).

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Deadlines in minutes B-0 B-l. B-2 B-3 B-4 B-5 15 110.8 17.0 10.9 9.8 '4.9 7.0 30 142.0 24.7 15, ô 13.0 6.9 8, 1 45 153.8 27.9 16.9 14.9 7.7 8.9 60 158.9 29.9 17 * 1. 15.2 7.9 9.7 90 165.8 30.2 17.3 15.4 8.2 10.1

The results of this experiment show that all of the flavorings used, not in accordance with the invention, have a repellency such that this almost completely cancels the insecticidal effectiveness of the baits.

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Claims (12)

1. Composition for use as bait, intended for the fight against synanthropic flies, comprising a food which is or which comprises an amino acid vector, characterized in that it comprises a flavoring agent chosen from juices, syrups and natural concentrates of raspberry and / or blackcurrant. 2. Composition according to claim 1, characterized in that it contains approximately 0.5 to 10% by weight of flavoring agent, these proportions relating to the total weight of said composition. 3. Composition according to Claims 1 and 2, characterized in that it contains approximately 1 to 8% by weight of flavoring agent, these proportions relating to the total weight of said composition. 4. Composition according to any one of claims 1 to 3, characterized in that the proportion of amino acid vector in the food is between approximately 2 and 100% by weight of the food, these proportions relating to the total weight of said composition. 5. Composition according to any one of claims * 1 to 4, characterized in that the proportion of amino acid vector 4 in the food is between about 10 and 100% by weight of the food. 6. Composition according to any one of Claims 1 to 5, characterized in that it contains approximately 0.01 to 8% by weight, relative to the total weight of the composition, of an insecticidal agent. »I - 15 - 7. Composition according to any one of claims 1 to 6, characterized in that the insecticidal agent is chosen from N-substituted aromatic or hetero-aromatic carbamic esters and organophosphorus compounds. 8. Composition according to any one of claims% 1 to 7, characterized in that the insecticidal agent is chosen from dimetilan and dioxacarb and mixtures of these compounds. 9. Composition according to any one of claims 1 to 8, characterized in that the insecticidal agent is chosen from malathion, azamethiphos, fenitro-thion and binary and ternary mixtures of these compounds. 10. Composition according to any one of claims 1 to 9, characterized in that it is in the form of granules whose average particle size is between approximately 0.05 and 1 mm. 11. A method of obtaining a bait according to claim 6, characterized in that the insecticidal material is dissolved in a volatile solvent and sprayed on the powder mixture which contains the food and the flavoring agent * after which the product is evaporated. volatile solvent. J * 12. Use of a composition according to one of claims 1 to 15, in the fight against synanthropic flies. %