LT3368688T - Kompozicijos ir būdai, skirti modifikuotų citozinų nustatymui sekvenavimu - Google Patents
Kompozicijos ir būdai, skirti modifikuotų citozinų nustatymui sekvenavimuInfo
- Publication number
- LT3368688T LT3368688T LTEP16794858.7T LT16794858T LT3368688T LT 3368688 T LT3368688 T LT 3368688T LT 16794858 T LT16794858 T LT 16794858T LT 3368688 T LT3368688 T LT 3368688T
- Authority
- LT
- Lithuania
- Prior art keywords
- sequencing
- compositions
- methods
- modified cytosines
- determining modified
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6872—Methods for sequencing involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562248872P | 2015-10-30 | 2015-10-30 | |
| US201562257284P | 2015-11-19 | 2015-11-19 | |
| US201562271679P | 2015-12-28 | 2015-12-28 | |
| US201662300396P | 2016-02-26 | 2016-02-26 | |
| US201662325626P | 2016-04-21 | 2016-04-21 | |
| PCT/US2016/059447 WO2017075436A1 (en) | 2015-10-30 | 2016-10-28 | Compositions and methods for determining modified cytosines by sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LT3368688T true LT3368688T (lt) | 2021-03-10 |
Family
ID=57286864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTEP16794858.7T LT3368688T (lt) | 2015-10-30 | 2016-10-28 | Kompozicijos ir būdai, skirti modifikuotų citozinų nustatymui sekvenavimu |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US10619200B2 (lt) |
| EP (2) | EP3845668A1 (lt) |
| CN (2) | CN115927563A (lt) |
| LT (1) | LT3368688T (lt) |
| WO (1) | WO2017075436A1 (lt) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010037001A2 (en) | 2008-09-26 | 2010-04-01 | Immune Disease Institute, Inc. | Selective oxidation of 5-methylcytosine by tet-family proteins |
| ES2669214T3 (es) | 2011-12-13 | 2018-05-24 | Oslo Universitetssykehus Hf | Procedimientos y kits para la detección de estado de metilación |
| ES2669512T3 (es) | 2012-11-30 | 2018-05-28 | Cambridge Epigenetix Limited | Agente oxidante para nucleótidos modificados |
| IL286474B2 (en) | 2014-06-23 | 2023-11-01 | Massachusetts Gen Hospital | Genome-wide random identification of DSBS assessed by sequencing (guide-sequence) |
| CN108350453A (zh) | 2015-09-11 | 2018-07-31 | 通用医疗公司 | 核酸酶dsb的完全查询和测序(find-seq) |
| EP3355939A4 (en) | 2015-09-30 | 2019-04-17 | Trustees of Boston University | MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES |
| US10260088B2 (en) * | 2015-10-30 | 2019-04-16 | New England Biolabs, Inc. | Compositions and methods for analyzing modified nucleotides |
| LT3368688T (lt) * | 2015-10-30 | 2021-03-10 | New England Biolabs, Inc. | Kompozicijos ir būdai, skirti modifikuotų citozinų nustatymui sekvenavimu |
| EP4528275A3 (en) | 2016-08-10 | 2025-05-14 | Grail, Inc. | Methods of preparing dual-indexed dna libraries for bisulfite conversion sequencing |
| US10155939B1 (en) | 2017-06-15 | 2018-12-18 | New England Biolabs, Inc. | Method for performing multiple enzyme reactions in a single tube |
| CN107447044A (zh) * | 2017-08-09 | 2017-12-08 | 华子昂 | 一种检测大肠杆菌λ噬菌体全基因组碱基修饰单倍型的方法 |
| WO2019040650A1 (en) | 2017-08-23 | 2019-02-28 | The General Hospital Corporation | GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES HAVING MODIFIED PAM SPECIFICITY |
| WO2019075197A1 (en) | 2017-10-11 | 2019-04-18 | The General Hospital Corporation | METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES |
| GB2587939B (en) | 2018-04-02 | 2023-06-14 | Grail Llc | Methylation markers and targeted methylation probe panels |
| JP7460539B2 (ja) | 2018-04-17 | 2024-04-02 | ザ ジェネラル ホスピタル コーポレイション | 核酸を結合、修飾、および切断する物質の基質選択性および部位のためのｉｎ ｖｉｔｒｏでの高感度アッセイ |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| CA3111887A1 (en) | 2018-09-27 | 2020-04-02 | Grail, Inc. | Methylation markers and targeted methylation probe panel |
| JP7717608B2 (ja) * | 2019-05-31 | 2025-08-04 | フリーノム ホールディングス，インク． | メチル化核酸の高深度シーケンシングのための方法とシステム |
| CN110358817B (zh) * | 2019-06-25 | 2021-03-16 | 武汉大学 | 脱氨酶辅助的dna中5-羧基胞嘧啶修饰单碱基分辨率定位分析方法 |
| CA3150630A1 (en) * | 2019-09-09 | 2021-03-18 | Katherine E. Varley | Targeted sequencing to detect and quantify low levels of methylated dna |
| WO2021092476A1 (en) | 2019-11-06 | 2021-05-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems for analyzing nucleic acid molecules |
| WO2021133859A1 (en) * | 2019-12-23 | 2021-07-01 | Active Motif, Inc. | Methods and kits for detection of n-4-acetyldeoxycytidine in dna |
| CN110982883A (zh) * | 2019-12-30 | 2020-04-10 | 西安交通大学 | 一种高通量单细胞基因组5-羟甲基嘧啶单分子可视化分析方法 |
| CN113088562A (zh) * | 2020-01-08 | 2021-07-09 | 深圳华大基因股份有限公司 | 一种新型的低起始量dna甲基化建库方法 |
| WO2022023753A1 (en) | 2020-07-30 | 2022-02-03 | Cambridge Epigenetix Limited | Compositions and methods for nucleic acid analysis |
| CN112708661B (zh) * | 2021-01-27 | 2022-07-12 | 西安交通大学 | 一种细胞内5-醛基尿嘧啶的可视化分析方法及系统 |
| EP4294936A1 (en) | 2021-02-19 | 2023-12-27 | New England Biolabs, Inc. | Compositions and methods for labeling modified nucleotides in nucleic acids |
| GB2621782B (en) * | 2021-05-05 | 2024-08-14 | Univ Leland Stanford Junior | Methods and systems for analyzing nucleic acid molecules |
| US20250283170A1 (en) * | 2021-07-12 | 2025-09-11 | The Trustees Of The University Of Pennsylvania | Modified adapters for enzymatic dna deamination and methods of use thereof for epigenetic sequencing of free and immobilized dna |
| GB202112817D0 (en) * | 2021-09-08 | 2021-10-20 | Mitra Bio Ltd | Methods, compositions, and kits for characterizing skins |
| CN113846070B (zh) * | 2021-10-18 | 2023-05-16 | 翌圣生物科技(上海)股份有限公司 | 高活性的mTET2酶突变体、其编码DNA及其应用 |
| CN114134205B (zh) * | 2021-11-25 | 2023-11-24 | 武汉大学 | 脱氨酶介导的dna中n4-甲基胞嘧啶的单碱基分辨率定位分析方法 |
| WO2023175013A1 (en) | 2022-03-15 | 2023-09-21 | Illumina, Inc. | Methods for preparing signals for concurrent sequencing |
| AU2023233839A1 (en) | 2022-03-15 | 2024-01-18 | Illumina, Inc. | Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides for methylation detection |
| CN115323035B (zh) * | 2022-10-18 | 2023-02-10 | 翌圣生物科技(上海)股份有限公司 | 一种检测tet酶氧化能力的方法 |
| WO2024235696A1 (en) * | 2023-05-12 | 2024-11-21 | F. Hoffmann-La Roche Ag | Enzymatic conversion of methylated nucleic acids for sequencing |
| WO2024256581A1 (en) | 2023-06-14 | 2024-12-19 | Illumina, Inc. | Determination of modified cytosines |
| WO2025129193A1 (en) | 2023-12-15 | 2025-06-19 | New England Biolabs, Inc. | Glycosyltransferases and related methods |
| WO2026006746A2 (en) | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Nucleic acid preparation and analysis techniques |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9389233B2 (en) * | 2008-06-12 | 2016-07-12 | Institute National De La Sante Et De La Recherche Medicale (Inserm) | TET2 as a diagnostic and pronostic marker in hematopoietic neoplasms |
| WO2010037001A2 (en) | 2008-09-26 | 2010-04-01 | Immune Disease Institute, Inc. | Selective oxidation of 5-methylcytosine by tet-family proteins |
| US9175338B2 (en) * | 2008-12-11 | 2015-11-03 | Pacific Biosciences Of California, Inc. | Methods for identifying nucleic acid modifications |
| WO2012034170A1 (en) * | 2010-09-13 | 2012-03-22 | Clinical Genomics Pty. Ltd | Epigenetic markers of colorectal cancers and diagnostic methods using the same |
| EP2694686B2 (en) | 2011-04-06 | 2023-07-19 | The University of Chicago | COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC) |
| US10081827B2 (en) * | 2012-03-15 | 2018-09-25 | New England Biolabs, Inc. | Mapping cytosine modifications |
| JP6224689B2 (ja) | 2012-03-15 | 2017-11-01 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シトシンとこれの修飾物とを識別するための、およびメチローム分析のための方法および組成物 |
| WO2013163207A1 (en) | 2012-04-24 | 2013-10-31 | Pacific Biosciences Of California, Inc. | Identification of 5-methyl-c in nucleic acid templates |
| US10597647B2 (en) | 2013-06-28 | 2020-03-24 | New England Biolabs, Inc. | Compositions and methods for identifying hydroxymethylcytosine in a DNA |
| WO2016183289A1 (en) * | 2015-05-12 | 2016-11-17 | Wake Forest University Health Services | Identification of genetic modifications |
| LT3368688T (lt) * | 2015-10-30 | 2021-03-10 | New England Biolabs, Inc. | Kompozicijos ir būdai, skirti modifikuotų citozinų nustatymui sekvenavimu |
| US10260088B2 (en) * | 2015-10-30 | 2019-04-16 | New England Biolabs, Inc. | Compositions and methods for analyzing modified nucleotides |
-
2016
- 2016-10-28 LT LTEP16794858.7T patent/LT3368688T/lt unknown
- 2016-10-28 CN CN202211163854.2A patent/CN115927563A/zh active Pending
- 2016-10-28 WO PCT/US2016/059447 patent/WO2017075436A1/en not_active Ceased
- 2016-10-28 EP EP20214863.1A patent/EP3845668A1/en active Pending
- 2016-10-28 EP EP16794858.7A patent/EP3368688B1/en active Active
- 2016-10-28 CN CN201680077157.1A patent/CN108699598B/zh active Active
- 2016-10-28 US US15/771,275 patent/US10619200B2/en active Active
-
2017
- 2017-02-24 US US15/441,431 patent/US10227646B2/en active Active
-
2018
- 2018-12-12 US US16/217,819 patent/US11124825B2/en active Active
-
2021
- 2021-08-17 US US17/445,286 patent/US20210388433A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20170198344A1 (en) | 2017-07-13 |
| EP3845668A1 (en) | 2021-07-07 |
| CN108699598A (zh) | 2018-10-23 |
| US20210388433A1 (en) | 2021-12-16 |
| US11124825B2 (en) | 2021-09-21 |
| US20180312914A1 (en) | 2018-11-01 |
| WO2017075436A1 (en) | 2017-05-04 |
| EP3368688B1 (en) | 2021-01-27 |
| US20190100796A1 (en) | 2019-04-04 |
| US10619200B2 (en) | 2020-04-14 |
| CN108699598B (zh) | 2022-11-15 |
| CN115927563A (zh) | 2023-04-07 |
| EP3368688A1 (en) | 2018-09-05 |
| US10227646B2 (en) | 2019-03-12 |
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