LT3167065T - Dviguba cistroninė bakterinė raiškos sistema - Google Patents
Dviguba cistroninė bakterinė raiškos sistemaInfo
- Publication number
- LT3167065T LT3167065T LTEP15745247.5T LT15745247T LT3167065T LT 3167065 T LT3167065 T LT 3167065T LT 15745247 T LT15745247 T LT 15745247T LT 3167065 T LT3167065 T LT 3167065T
- Authority
- LT
- Lithuania
- Prior art keywords
- expression system
- bacterial expression
- cistronic
- double
- double cistronic
- Prior art date
Links
- 230000001580 bacterial effect Effects 0.000 title 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/34—Cucurbitaceae, e.g. bitter melon, cucumber or watermelon
- A01H6/342—Citrullus lanatus [watermelon]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/27—Erwinia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24029—Aureolysin (3.4.24.29)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/515—Complete light chain, i.e. VL + CL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Developmental Biology & Embryology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN2245MU2014 | 2014-07-09 | ||
| PCT/IB2015/055189 WO2016005931A1 (en) | 2014-07-09 | 2015-07-09 | Dual cistronic bacterial expression system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LT3167065T true LT3167065T (lt) | 2020-07-27 |
Family
ID=53773474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTEP15745247.5T LT3167065T (lt) | 2014-07-09 | 2015-07-09 | Dviguba cistroninė bakterinė raiškos sistema |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US10329571B2 (lt) |
| EP (1) | EP3167065B1 (lt) |
| JP (1) | JP6748061B2 (lt) |
| CN (1) | CN106661588B (lt) |
| AU (1) | AU2015287296A1 (lt) |
| BR (1) | BR112017000389A2 (lt) |
| CA (1) | CA2954594A1 (lt) |
| CY (1) | CY1123303T1 (lt) |
| DK (1) | DK3167065T3 (lt) |
| ES (1) | ES2808906T3 (lt) |
| HR (1) | HRP20200996T1 (lt) |
| HU (1) | HUE050300T2 (lt) |
| LT (1) | LT3167065T (lt) |
| MX (1) | MX378585B (lt) |
| PH (1) | PH12017500047A1 (lt) |
| PL (1) | PL3167065T3 (lt) |
| PT (1) | PT3167065T (lt) |
| RU (1) | RU2732148C2 (lt) |
| SI (1) | SI3167065T1 (lt) |
| WO (1) | WO2016005931A1 (lt) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6435770B2 (ja) * | 2014-01-21 | 2018-12-12 | 東ソー株式会社 | シグナルペプチドをコードするポリヌクレオチドおよびそれを用いたタンパク質の製造方法 |
| WO2017021819A1 (en) * | 2015-07-31 | 2017-02-09 | Dr. Reddy’S Laboratories Limited | Process for preparation of protein or peptide |
| JP7282689B2 (ja) * | 2017-05-19 | 2023-05-29 | カウンスィル オブ サイエンティフィック アンド インダストリアル リサーチ | リフォールディングした組み換えヒト化ラニビズマブの製造方法 |
| CN111094566B (zh) * | 2017-08-23 | 2024-05-24 | 诺华股份有限公司 | 多拷贝基因蛋白表达系统 |
| CN113631567B (zh) * | 2018-07-06 | 2025-02-07 | 瓦克化学股份公司 | 在发酵方法中释放重组蛋白的细菌菌株 |
| JP7762935B2 (ja) * | 2019-01-31 | 2025-10-31 | Spiber株式会社 | 組換え細胞、発現構築物、及び組換えタンパク質の製造方法 |
| WO2020202208A1 (en) * | 2019-04-02 | 2020-10-08 | Council Of Scientific & Industrial Research | Cloning and expression of in-vivo refolded antibody fragment |
| CN114774453B (zh) * | 2022-03-14 | 2023-04-21 | 湖北大学 | 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用 |
| CN116334118A (zh) * | 2023-02-23 | 2023-06-27 | 四川大学 | 一种原核体系中单启动子控制多个颗粒蛋白亚基组装同源等同体在胞内恒定比例表达并共自组装的方法 |
| CN118995554B (zh) * | 2024-09-09 | 2025-07-04 | 江南大学 | 一种大肠杆菌整合表达体系及其在酶高效表达中的应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993006217A1 (en) | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
| ATE405650T1 (de) * | 2000-12-14 | 2008-09-15 | Genentech Inc | Produktion von ganzen antikörpern in prokaryontischen zellen |
| MXPA04001566A (es) | 2001-08-27 | 2004-05-17 | Genentech Inc | Un sistema para expresion y ensamblado de anticuerpos. |
| DK2046382T3 (en) * | 2006-07-10 | 2016-12-12 | Esbatech Alcon Biomed Res Unit | scFv Antibodies which passes through the epithelial and / or endothelial layer |
| RU2413769C1 (ru) * | 2009-12-24 | 2011-03-10 | Учреждение Российской академии наук Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pET-32a, КОДИРУЮЩАЯ ГЕН ЛИГАНД-СВЯЗЫВАЮЩЕГО ДОМЕНА РЕЦЕПТОРА II ТИПА ТРАНСФОРМИРУЮЩЕГО ФАКТОРА РОСТА-β ЧЕЛОВЕКА (TβRII), ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ СЛИТНОГО БЕЛКА ТИОРЕДОКСИН/TβRII И СПОСОБ РЕНАТУРАЦИИ И ОЧИСТКИ ЦЕЛЕВОГО БЕЛКА TβRII |
| GB201002730D0 (en) * | 2010-02-18 | 2010-04-07 | Uni I Oslo | Product |
| US8853380B2 (en) | 2010-06-04 | 2014-10-07 | Lupin Limited | Modified SAK gene for the production of recombinant proteins |
| AU2014225272B2 (en) * | 2013-03-08 | 2015-10-22 | Neuclone Biologics Pty Ltd | A cell expression system |
| MX368142B (es) * | 2013-04-30 | 2019-09-20 | Intas Pharmaceuticals Ltd | Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab. |
| TW201534726A (zh) * | 2013-07-03 | 2015-09-16 | Halozyme Inc | 熱穩定ph20玻尿酸酶變異體及其用途 |
-
2015
- 2015-07-09 HR HRP20200996TT patent/HRP20200996T1/hr unknown
- 2015-07-09 AU AU2015287296A patent/AU2015287296A1/en not_active Abandoned
- 2015-07-09 HU HUE15745247A patent/HUE050300T2/hu unknown
- 2015-07-09 CN CN201580037078.3A patent/CN106661588B/zh not_active Expired - Fee Related
- 2015-07-09 RU RU2017102578A patent/RU2732148C2/ru active
- 2015-07-09 PL PL15745247T patent/PL3167065T3/pl unknown
- 2015-07-09 MX MX2017000332A patent/MX378585B/es unknown
- 2015-07-09 LT LTEP15745247.5T patent/LT3167065T/lt unknown
- 2015-07-09 BR BR112017000389A patent/BR112017000389A2/pt not_active Application Discontinuation
- 2015-07-09 US US15/324,105 patent/US10329571B2/en not_active Expired - Fee Related
- 2015-07-09 WO PCT/IB2015/055189 patent/WO2016005931A1/en not_active Ceased
- 2015-07-09 ES ES15745247T patent/ES2808906T3/es active Active
- 2015-07-09 DK DK15745247.5T patent/DK3167065T3/da active
- 2015-07-09 SI SI201531263T patent/SI3167065T1/sl unknown
- 2015-07-09 PT PT157452475T patent/PT3167065T/pt unknown
- 2015-07-09 CA CA2954594A patent/CA2954594A1/en not_active Abandoned
- 2015-07-09 EP EP15745247.5A patent/EP3167065B1/en active Active
- 2015-07-09 JP JP2017500849A patent/JP6748061B2/ja active Active
-
2017
- 2017-01-06 PH PH12017500047A patent/PH12017500047A1/en unknown
-
2019
- 2019-04-24 US US16/393,338 patent/US11365418B2/en active Active
-
2020
- 2020-06-25 CY CY20201100580T patent/CY1123303T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US11365418B2 (en) | 2022-06-21 |
| MX2017000332A (es) | 2017-07-28 |
| PT3167065T (pt) | 2020-07-01 |
| JP2017520261A (ja) | 2017-07-27 |
| RU2732148C2 (ru) | 2020-09-11 |
| CN106661588A (zh) | 2017-05-10 |
| PH12017500047A1 (en) | 2017-05-22 |
| PL3167065T3 (pl) | 2020-11-16 |
| WO2016005931A1 (en) | 2016-01-14 |
| CN106661588B (zh) | 2020-11-06 |
| HUE050300T2 (hu) | 2020-11-30 |
| JP6748061B2 (ja) | 2020-08-26 |
| US10329571B2 (en) | 2019-06-25 |
| EP3167065A1 (en) | 2017-05-17 |
| CA2954594A1 (en) | 2016-01-14 |
| BR112017000389A2 (pt) | 2018-01-23 |
| ES2808906T3 (es) | 2021-03-02 |
| CY1123303T1 (el) | 2021-12-31 |
| US20170159060A1 (en) | 2017-06-08 |
| HRP20200996T1 (hr) | 2021-01-08 |
| EP3167065B1 (en) | 2020-03-25 |
| MX378585B (es) | 2025-03-10 |
| US20190309313A1 (en) | 2019-10-10 |
| SI3167065T1 (sl) | 2020-09-30 |
| AU2015287296A1 (en) | 2017-02-02 |
| DK3167065T3 (da) | 2020-07-06 |
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