LT2002133A - Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro - Google Patents
Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitroInfo
- Publication number
- LT2002133A LT2002133A LT2002133A LT2002133A LT2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A LT 2002133 A LT2002133 A LT 2002133A
- Authority
- LT
- Lithuania
- Prior art keywords
- chaperons
- refolding
- grpe
- dnak
- dnaj
- Prior art date
Links
- 102100023737 GrpE protein homolog 1, mitochondrial Human genes 0.000 title abstract 2
- 102000004447 HSP40 Heat-Shock Proteins Human genes 0.000 title abstract 2
- 108010042283 HSP40 Heat-Shock Proteins Proteins 0.000 title abstract 2
- 101000829489 Homo sapiens GrpE protein homolog 1, mitochondrial Proteins 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 241000589496 Meiothermus ruber Species 0.000 title 1
- 241000921347 Meiothermus Species 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000030788 protein refolding Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention claims protein refolding system which includes thermophilic chaperons cloned from mezophilic microorganism Meiothermus rubber. The chaperons mentioned above are characterized by increased thermal stability and refolding activity compared to chaperons known previously. The refolding system claimed (the refolding buffer) consists of denaturated protein, neorganic salts, the mixture of three purified thermophilic chaperons (DnaK, DnaJ and GrpE) and adenosintriphosphate. The invention belongs to biotechnology and may be useful in restoring in vitro the active structure of native and recombinant proteins in the process of their preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LT2002133A LT5053B (en) | 2002-12-24 | 2002-12-24 | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LT2002133A LT5053B (en) | 2002-12-24 | 2002-12-24 | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LT2002133A true LT2002133A (en) | 2003-07-25 |
| LT5053B LT5053B (en) | 2003-09-25 |
Family
ID=27730946
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LT2002133A LT5053B (en) | 2002-12-24 | 2002-12-24 | Chaperons DnaK, DnaJ and GrpE from Meiothermus ruber having increased thermal stability and system and process for refolding proteins in vitro |
Country Status (1)
| Country | Link |
|---|---|
| LT (1) | LT5053B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7939711B2 (en) | 2006-09-11 | 2011-05-10 | The Chinese University Of Hong Kong | Abiotic stress tolerance conferred by J-domain containing proteins |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3344618B2 (en) | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | Chaperone expression plasmid |
| CA2292845A1 (en) | 1997-07-24 | 1999-02-04 | Myriam Marlenne Altamirano | Refolding method using a foldase and a chaperone |
| JP4249832B2 (en) | 1998-12-28 | 2009-04-08 | タカラバイオ株式会社 | Trigger factor expression plasmid |
| EP1149909A1 (en) | 2000-04-28 | 2001-10-31 | Boehringer Mannheim Gmbh | Methods for regulating protein conformation using molecular chaperones |
-
2002
- 2002-12-24 LT LT2002133A patent/LT5053B/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| LT5053B (en) | 2003-09-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM9A | Lapsed patents |
Effective date: 20041224 |