KR900006505A - 박테리아의 배양방법 - Google Patents
박테리아의 배양방법 Download PDFInfo
- Publication number
- KR900006505A KR900006505A KR1019890014288A KR890014288A KR900006505A KR 900006505 A KR900006505 A KR 900006505A KR 1019890014288 A KR1019890014288 A KR 1019890014288A KR 890014288 A KR890014288 A KR 890014288A KR 900006505 A KR900006505 A KR 900006505A
- Authority
- KR
- South Korea
- Prior art keywords
- bacteria
- urea
- rhodococcus rhodochrous
- iii
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title claims 5
- 238000000034 method Methods 0.000 claims 4
- 241000187693 Rhodococcus rhodochrous Species 0.000 claims 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 3
- 239000004202 carbamide Substances 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- 229910001429 cobalt ion Inorganic materials 0.000 claims 2
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 108090001042 Hydro-Lyases Proteins 0.000 claims 1
- 102000004867 Hydro-Lyases Human genes 0.000 claims 1
- 108010024026 Nitrile hydratase Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 238000012364 cultivation method Methods 0.000 claims 1
- 238000012136 culture method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 150000002825 nitriles Chemical class 0.000 claims 1
- 125000005245 nitryl group Chemical group [N+](=O)([O-])* 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (5)
- 나트릴 수화효소를 생산할 수 있는 Rhodococcus rhodochrous 박테리아를 배양시켜 니트릴수화효소의 활성을 갖게 되는 박테리아 세포제조시에 그 배양배지에다 우레아 및 다음 구조식(I) 내지 (III)으로 나타낸 그 유도체를 중 적어도 하나와 코발트 이온을 첨가시켜서 되어지는 Rhodococcus rhodochrous종 박테리아의 배양방법.R1R2NCONR3R4(I)윗식에서, R1, R2, R3와 R4는 각각 -H, -CH3, 또는 -C2H5일 수 있지만 모든 치환기가 -H가 되지는 않고,R5R6NCOOC2H5(II)윗식에서 R5과 R6는 각각 -H, -CH3또는 -C2H5이다.NH2CSNH2(III)
- 제1항에 있어서, 니트릴 수화효소를 생산해 낼 수 있는 Rhodococcus rhodochrous 박테리아는 균주 J-1(FERM BP-1478)인 것을 특징으로 하는 박테리아의 배양방법.
- 제1항에 있어서, 우레아가 배양 배지에 첨가되어짐을 특징으로 하는 박테리아의 배양방법.
- 제1항에 있어서, 배지에서의 우레아나 그 유도체의 전체 농도는 1 내지 30g/ℓ인 것을 특징으로 하는 박테리아의 배양방법.
- 제1항에 있어서, 코발트 이온의 농도는 CoCl2를 기초로 계산하여 5 내지 15mg/ℓ인 것을 특징으로 하는 박테리아의 배양방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63252645A JPH0753103B2 (ja) | 1988-10-06 | 1988-10-06 | 細菌の培養法 |
| JP63-252645 | 1988-10-06 | ||
| JP1-46818 | 1989-02-28 | ||
| JP1046818A JPH0753104B2 (ja) | 1989-02-28 | 1989-02-28 | 細菌の培養法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR900006505A true KR900006505A (ko) | 1990-05-08 |
| KR100187512B1 KR100187512B1 (ko) | 1999-04-01 |
Family
ID=26386951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019890014288A Expired - Lifetime KR100187512B1 (ko) | 1988-10-06 | 1989-10-05 | 박테리아의 배양방법 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5089411A (ko) |
| EP (1) | EP0362829B1 (ko) |
| KR (1) | KR100187512B1 (ko) |
| CN (1) | CN1039030C (ko) |
| AR (1) | AR241936A1 (ko) |
| AT (1) | ATE124992T1 (ko) |
| AU (1) | AU622489B2 (ko) |
| CZ (1) | CZ280901B6 (ko) |
| DE (1) | DE68923419T2 (ko) |
| ES (1) | ES2076944T3 (ko) |
| PL (1) | PL161863B1 (ko) |
| RU (1) | RU1838408C (ko) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5648256A (en) * | 1990-02-28 | 1997-07-15 | Nitto Chemical Industry Co., Ltd. | Gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant |
| US5264361A (en) * | 1991-03-18 | 1993-11-23 | Lonza Ltd. | Microbiological process for the production of 6-hydroxypicolinic acid |
| US5753472A (en) * | 1991-05-02 | 1998-05-19 | Nitto Chemical Industry Co. Ltd. | DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant |
| US5270203A (en) * | 1992-03-13 | 1993-12-14 | Lonza Ltd. | Biologically pure culture of Alcaligenes faecalis DSM 6335 |
| JP3409353B2 (ja) * | 1992-04-30 | 2003-05-26 | 住友化学工業株式会社 | アミド化合物の製造方法および使用される微生物 |
| RU2053300C1 (ru) * | 1993-12-17 | 1996-01-27 | Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов | Штамм бактерий rhodococcus rhodochrous - продуцент нитрилгидратазы |
| TW422882B (en) * | 1994-02-01 | 2001-02-21 | Sumitomo Chemical Co | Process for production of amide compounds using microorganism |
| AU707650B2 (en) * | 1995-08-09 | 1999-07-15 | Ciba Specialty Chemicals Water Treatments Limited | Processes for the production of amidase |
| JP3491669B2 (ja) * | 1997-07-11 | 2004-01-26 | 理化学研究所 | 光代謝制御 |
| CA2294621C (en) * | 1997-07-22 | 2007-05-08 | Lonza Ag | Process for preparing amides |
| RU2160778C1 (ru) * | 1999-11-10 | 2000-12-20 | Федеральное государственное унитарное предприятие "Пермский завод им. С.М. Кирова" | Штамм бактерий bacillus сereus-продуцент нитрилгидратазы |
| EP1283256B1 (en) * | 2000-03-21 | 2007-07-25 | Mitsubishi Rayon Co., Ltd. | Method of culturing microorganisms |
| KR100407470B1 (ko) * | 2000-08-08 | 2003-11-28 | 동서석유화학주식회사 | 로도코커스 로도크로스의 유가식 회분 배양방법 및 상기배양방법에 의해 배양된 로도코커스 로도크로스를이용하여 니트릴 화합물로부터 아미드 화합물을 제조하는방법 |
| RU2196822C1 (ru) * | 2001-07-25 | 2003-01-20 | Институт экологии и генетики микроорганизмов Уральского отделения РАН | Штамм бактерий rhodococcus erythropolis - продуцент нитрилгидратазы |
| RU2223316C2 (ru) * | 2001-12-06 | 2004-02-10 | ФГУП "Пермский завод им. С.М.Кирова" | ШТАММ БАКТЕРИЙ rhodococcus ruber - ПРОДУЦЕНТ НИТРИЛГИДРАТАЗЫ |
| FR2835531B1 (fr) * | 2002-02-06 | 2004-12-03 | Snf Sa | Procede pour la fabrication d'amides en presence d'une nitrile hydratase d'origine microbiologique et nouveau microorganisme |
| DE10315376B4 (de) * | 2003-04-03 | 2005-07-14 | Stockhausen Gmbh | Verfahren zur Kultivierung des Nitrilhydratase-produzierenden Stammes RHODOCOCCUS RHODOCHROUS M33 |
| GB0327900D0 (en) | 2003-12-02 | 2004-01-07 | Ciba Spec Chem Water Treat Ltd | Process for preparing unsaturated amides and carboxylic acids |
| DE602004024329D1 (de) * | 2003-12-02 | 2010-01-07 | Ciba Spec Chem Water Treat Ltd | Herstellung von amiden |
| GB0327901D0 (en) | 2003-12-02 | 2004-01-07 | Ciba Spec Chem Water Treat Ltd | Process for producing polymers |
| BRPI0417244B1 (pt) * | 2003-12-02 | 2015-05-26 | Ciba Spec Chem Water Treat Ltd | Métodos para cultivo e armazenamento de rhodococcus rhodochrous, bem como para melhorar atividade biocatalítica do mesmo, e processo para preparação de amida |
| GB0327907D0 (en) * | 2003-12-02 | 2004-01-07 | Ciba Spec Chem Water Treat Ltd | Microorganism |
| DE102004013824A1 (de) | 2004-03-20 | 2005-10-13 | Degussa Ag | Nitrilhydratasen aus Rhodococcus opacus |
| WO2007090122A2 (en) * | 2006-01-30 | 2007-08-09 | Georgia State University Research Foundation, Inc. | Induction and stabilization of enzymatic activity in microorganisms |
| US7943549B2 (en) | 2007-04-02 | 2011-05-17 | Georgia State University Research Foundation, Inc. | Biological-based catalyst to delay plant development processes |
| KR101808496B1 (ko) | 2009-07-31 | 2017-12-12 | 백스터 인터내셔널 인코포레이티드 | Adamts 단백질 발현을 위한 세포 배양 배지 |
| CA2787334A1 (en) * | 2010-01-25 | 2011-07-28 | George E. Pierce | Induction and stabilization of enzymatic activity in microorganisms |
| CN102286399B (zh) * | 2011-07-08 | 2013-07-17 | 山东大学 | 可代谢二苯并呋喃的红球菌及其应用 |
| RU2520870C1 (ru) | 2012-12-27 | 2014-06-27 | Кемира Оюй | Штамм бактерий rhodococcus aetherivorans bkm ac-2610d - продуцент нитрилгидратазы, способ его культивирования и способ получения акриламида |
| FR3002542B1 (fr) * | 2013-02-28 | 2016-01-22 | Servier Lab | Procede de synthese enzymatique de l'acide (7s) 3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene 7-carboxylique et application a la synthese de l'ivabradine et de ses sels |
| JP6454320B2 (ja) | 2013-03-14 | 2019-01-16 | ジョージア・ステイト・ユニバーシティ・リサーチ・ファウンデーション・インコーポレイテッドGeorgia State University Research Foundation, Inc. | 植物における低温障害反応の防止または遅延 |
| MX361278B (es) | 2013-03-14 | 2018-12-03 | Univ Georgia State Res Found | Inhibición o reducción del crecimiento de hongos. |
| WO2016050819A1 (en) * | 2014-09-30 | 2016-04-07 | Basf Se | Method for preparing an acrylamide solution having a low acrylic acid concentration |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4629700A (en) * | 1983-11-30 | 1986-12-16 | Standard Oil Company (Indiana) | Selective conversion of cyano compounds to amides and carboxylic acids |
-
1989
- 1989-10-03 CZ CS895621A patent/CZ280901B6/cs not_active IP Right Cessation
- 1989-10-04 DE DE68923419T patent/DE68923419T2/de not_active Expired - Lifetime
- 1989-10-04 ES ES89118429T patent/ES2076944T3/es not_active Expired - Lifetime
- 1989-10-04 AU AU42573/89A patent/AU622489B2/en not_active Expired
- 1989-10-04 EP EP89118429A patent/EP0362829B1/en not_active Expired - Lifetime
- 1989-10-04 AT AT89118429T patent/ATE124992T1/de not_active IP Right Cessation
- 1989-10-05 RU SU894742197A patent/RU1838408C/ru active
- 1989-10-05 US US07/417,259 patent/US5089411A/en not_active Expired - Lifetime
- 1989-10-05 AR AR89315099A patent/AR241936A1/es active
- 1989-10-05 KR KR1019890014288A patent/KR100187512B1/ko not_active Expired - Lifetime
- 1989-10-06 PL PL89281740A patent/PL161863B1/pl unknown
- 1989-10-06 CN CN89108175A patent/CN1039030C/zh not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0362829A2 (en) | 1990-04-11 |
| EP0362829B1 (en) | 1995-07-12 |
| PL161863B1 (en) | 1993-08-31 |
| CZ280901B6 (cs) | 1996-05-15 |
| ATE124992T1 (de) | 1995-07-15 |
| KR100187512B1 (ko) | 1999-04-01 |
| AR241936A1 (es) | 1993-01-29 |
| AU622489B2 (en) | 1992-04-09 |
| RU1838408C (ru) | 1993-08-30 |
| EP0362829A3 (en) | 1991-05-15 |
| US5089411A (en) | 1992-02-18 |
| CN1039030C (zh) | 1998-07-08 |
| ES2076944T3 (es) | 1995-11-16 |
| CN1041782A (zh) | 1990-05-02 |
| AU4257389A (en) | 1990-04-12 |
| DE68923419T2 (de) | 1996-01-04 |
| CZ562189A3 (en) | 1994-02-16 |
| DE68923419D1 (de) | 1995-08-17 |
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