KR20180087797A - Composition for prevention of hair loss and improvement of hair growth comprising acetoxychavicol acetate - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate as an active ingredient. Specifically, the present invention relates to a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, and a health functional food for preventing hair loss or promoting hair growth comprising acetoxy chive acetate as an active ingredient.
The acetoxychivicol acetate of the present invention is excellent in the effect of promoting the growth of hair follicles, so that the effect of preventing hair loss and accelerating hair growth is not only excellent but also is derived from natural products and has no side effects, Composition, quasi-drug composition, cosmetic composition, and health functional food.

Description

[0001] The present invention relates to a composition for preventing hair loss and promoting hair growth comprising acetoxycarbicylate as an active ingredient,

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate as an active ingredient.

Specifically, the present invention relates to a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, and a health functional food for preventing hair loss or promoting hair growth comprising acetoxy chive acetate as an active ingredient.

Hair loss refers to a state in which there is no hair on the area where hair should normally exist, and generally means that the hair of the scalp (thick hair) is lost. Hair loss is not only caused by aging, but also occurs frequently in young ages due to the combination of various factors. Factors of hair loss include specific factors such as genetic constitution, internal factors such as the action of male hormone, stress in daily life, dietary habits, accumulation of lipid peroxidation in the scalp, and the like. In recent years, as well as male hair loss, female hair loss is gradually spreading, including obesity hair loss, and many types of hair loss remedy, hair restoration and the like are on the market in order to improve such hair loss phenomenon.

Minoxidil, which is a typical hair loss treatment drug on the market, was originally developed as a hypertension treatment drug for lowering blood pressure. However, it has become more famous as a hair growth drug due to side effects due to side effects. However, the use of minoxidil is known to cause side effects such as scleroderma, erythema, keratin, contact dermatitis, and hirsutism (J Korean Med Assoc 2013 January; 56 (1): 45-54).

Another representative hair loss treatment agent, Propesia, inhibits the conversion of testosterone to dihydrotestosterone (DHT) by blocking the 5-alpha-reductase type 2 enzyme, which plays an important role in the pathogenesis of male alopecia It is known to improve or inhibit the progression of hair loss. Propecia is known to have excellent anti-hair loss effect, but the reagent used in the preparation thereof is expensive and toxic, and it acts in a manner to artificially block the production of steroid hormone, so that various side effects There is a danger. Therefore, it is still required to develop a safe therapeutic agent which is excellent in hair loss prevention and hair growth promoting effect, and has no side effects.

The present invention provides a composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate as an active ingredient.

Specifically, the present invention aims to provide a pharmaceutical composition, a quasi-drug composition, a cosmetic composition and a health functional food for preventing hair loss or promoting hair growth comprising acetoxy chive acetate as an active ingredient.

The present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate or a pharmaceutically acceptable salt thereof as an active ingredient.

[Chemical Formula 1]

In the present invention, the compound of Chemical Formula 1 may be chemically synthesized or separated from natural materials, and those commercially available in the market can be purchased and used. In the present invention, when the compound of Chemical Formula 1 is isolated from a plant, the species of the plant is not particularly limited, but preferably it can be isolated using a rhizome of Alpinia galanga . In addition, a plant extract containing acetoxycarbicylate at a high concentration can be also prepared.

The inventors of the present invention can produce extracts containing acetoxychivicacetate using a rhizome of Alpinia galanga ( Thailand). Specifically, according to an embodiment of the present invention, after 50 g of a large diameter rhizosphere body was cut out, 250 ml of 70% water-soluble ethanol was added and the ultrasonic extraction was repeated twice for 2 hours. After the extract obtained by the above method was filtered, the filtrate was concentrated under reduced pressure at 40 ° C to 50 ° C and then lyophilized to obtain 2.2g (yield: 4.4%) of 70% ethanol extract having a massive root diameter. The acetoxycarbicycloacetate content in the obtained extract was found to be 14.61% (FIG. 3).

In the case of the experiment using the single compound of acetoxychivicacetate in the example of the present invention, it was purchased from Abcam.

The compound of formula (I) of the present invention has an effect of promoting the growth of hair follicles.

Specifically, in the examples of the present invention, human hair follicle cells (n = 36) were divided into four groups, the first group being a control without treatment of the drug, the second group being treated with 500 nM testosterone, The test group was treated with 500 nM testosterone and 100 nM acetoxychivicol acetate and the group 4 was treated with 500 nM testosterone and 200 nM acetoxychivacole acetate to determine the size of the hair follicle in the test group treated with testosterone only While the test group treated with testosterone and acetoxycholicacetate showed that the size of the hair follicles was maintained to a similar degree to that of the control group not treated with testosterone (FIG. 2). This demonstrates that decrease in hair follicle size by testosterone and consequent hair growth inhibition is restored by acetoxy chrysocol acetate.

The compound of formula (I) of the present invention has an effect of inhibiting the production of reactive oxygen species (ROS) in skin cells.

The compound of formula (I) of the present invention has an effect of inhibiting the activity of NADPH oxidase (Nox) in skin cells.

The present invention also provides a pharmaceutical composition for preventing or treating hair loss comprising acetoxychavicol acetate or a pharmaceutically acceptable salt thereof as an active ingredient.

[Chemical Formula 1]

The term " pharmaceutically acceptable salt " as used in the present invention means a salt which can be used pharmacologically in a salt in which a cation and an anion are bound by an electrostatic attraction, Salts with organic bases, salts with inorganic acids, salts with organic acids, salts with basic or acidic amino acids, and the like. For example, the metal salt may be an alkali metal salt (sodium salt, potassium salt, etc.), an alkaline earth metal salt (calcium salt, magnesium salt, barium salt, etc.), an aluminum salt and the like; Examples of salts with organic bases include salts with organic bases such as triethylamine, pyridine, picoline, 2,6-lutidine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine, dicyclohexylamine, N, And the like; The salt with inorganic acid may be a salt with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid and the like; Salts with organic acids may be salts with formic acid, acetic acid, trifluoroacetic acid, phthalic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid and the like; Salts with basic amino acids may be salts with arginine, lysine, ornithine and the like; The salt with an acidic amino acid may be a salt with aspartic acid, glutamic acid and the like.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier in addition to the compound of the formula (I) or a pharmaceutically acceptable salt thereof as an active ingredient.

In the present invention, the type of the carrier is not particularly limited, and any carrier conventionally used in the art can be used. Examples of the carrier include, but are not limited to, saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, . These may be used alone or in combination of two or more.

In addition, the pharmaceutical composition of the present invention may be supplemented with other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostats, and may be used as fillers, extenders, wetting agents, disintegrants, , A binder, a lubricant, and the like may be additionally used.

In the pharmaceutical composition of the present invention, the compound of formula (I) or a pharmaceutically acceptable salt thereof may be contained in an amount of 0.00001 to 99.99% by weight based on the total weight of the pharmaceutical composition, preferably 1 % To 99.99% by weight, more preferably 10% by weight to 99.99% by weight, and further preferably 50% by weight to 99.99% by weight, and may be included in the total content of the pharmaceutical composition.

The pharmaceutical composition of the present invention may be formulated into various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of such oral dosage forms include troches, lozenges, tablets, aqueous suspensions, oily suspensions, prepared powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs .

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin; Excipients such as dicalcium phosphate and the like; Disintegrating agents such as corn starch or sweet potato starch; Magnesium stearate, calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax, and the like. Sweetening agents, fragrances, syrups, and the like may also be used. Furthermore, in the case of capsules, in addition to the above-mentioned substances, liquid carriers such as fatty oils can be further used.

Non-limiting examples of the parenteral preparation include injections, suppositories, respiratory inhalation powders, aerosol preparations for spraying, ointments, powder for application, oils, creams and the like.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, a sterilized aqueous solution, a non-aqueous solvent, a suspending agent, an emulsion, a lyophilized preparation, an external agent, and the like may be used. Examples of the non-aqueous solution and the suspension include propylene glycol, Polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like.

When the pharmaceutical composition of the present invention is formulated into an injection, the composition of the present invention is mixed with a stabilizer or a buffer in water to prepare a solution or suspension, which is formulated for unit administration of an ampoule or a vial can do.

When the pharmaceutical composition of the present invention is formulated into an aerosol formulation, a propellant or the like may be added together with the additive such that the water-dispersed concentrate or the wet powder is dispersed.

When the pharmaceutical composition of the present invention is formulated into an ointment, a cream, a powder for application, an oil, an external preparation for skin or the like, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, a polyethylene glycol, , Silica, talc, zinc oxide or the like as a carrier.

The pharmaceutically effective amount and the effective dose of the pharmaceutical composition of the present invention may be varied depending on the formulation method, administration method, administration time and / or administration route of the pharmaceutical composition, and the reaction to be achieved by the administration of the pharmaceutical composition The type and severity of the subject to be treated, the type of subject to be treated, age, weight, general health condition, symptom or degree of disease, sex, diet, excretion, drugs used simultaneously or simultaneously with the subject, And other factors well known in the medical arts, and those skilled in the art will readily determine and prescribe dosages that are effective for the desired treatment.

The administration of the pharmaceutical composition of the present invention may be administered once a day or divided into several doses. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. Taking into consideration all of the above factors, in such an amount as to obtain the maximum effect in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The route of administration and the mode of administration of the pharmaceutical composition of the present invention may be independent of each other, and may be arbitrarily selected depending on the route of administration and the mode of administration as long as the pharmaceutical composition can reach the desired site. The pharmaceutical composition may be administered orally or parenterally.

Examples of the parenteral administration method include intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, and the composition may be applied, sprayed or inhaled to the diseased site, It does not.

The pharmaceutical composition of the present invention can be preferably used as a method of directly applying or dispersing onto hair, skin or scalp.

As used herein, the term "hair" may include hair follicles and hair follicles, hair, eyelashes, eyelashes, whiskers and all parts of the body including hair follicles and hair follicles.

The present invention provides a quasi-drug composition for preventing hair loss or accelerating hair growth comprising acetoxy chivecale acetate or a pharmaceutically acceptable salt thereof as an active ingredient.

[Chemical Formula 1]

The term "quasi-drug product " used in the present invention refers to products that are less active than drugs among the products used for diagnosis, treatment, improvement, alleviation, treatment or prevention of diseases of human or animal. For example, Quasi-drugs are products that are used for the purpose of treating or preventing diseases or diseases of humans or animals, products which are mild or have no direct action on the human body.

When the compound of Chemical Formula 1 of the present invention is used as a quasi-drug additive, it may be added as it is or may be used together with other quasi-drugs or quasi-drugs, and may be suitably used according to a conventional method. The mixing ratio with other ingredients used together can be suitably determined according to the purpose of use (for example, prevention, improvement or therapeutic treatment).

Examples of the quasi-drug composition include, but are not limited to, scalp hair care compositions such as shampoo and rinse, body cleanser, foam, soap, mask, ointment, cream, lotion, essence, spray and the like.

The quasi-drug composition containing the compound of the formula (I) of the present invention or a pharmaceutically acceptable salt thereof as an active ingredient may preferably be a composition for scalp hair care.

As used herein, the term "composition for scalp hair care" means a composition that prevents or alleviates damage to the scalp and hair. The composition of the composition for scalp hair care may be, but not limited to, shampoo, rinse, essence, nutrient, serum, massage cream, lotion, pack.

The compound of Formula 1 or its pharmaceutically acceptable salt is not limited in its content but may be included in an amount of 0.001 to 50% by weight, preferably 10% by weight, based on the total weight of the quasi-drug composition.

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising acetoxy chavicol acetate or a cosmetically acceptable salt thereof, which is a compound represented by the following formula (1), as an active ingredient.

[Chemical Formula 1]

The term "cosmetically acceptable salt" as used in the present invention means a salt which can be used cosmetically among the salts in which the cation and the anion are bound by the electrostatic attraction, Specific examples include examples of the above-mentioned "pharmaceutically acceptable salts ".

The formulations of the cosmetic composition of the present invention may be in the form of solutions, shampoos, rinses, external ointments, creams, foams, nutritional lotions, suppositories, packs, supplements, emulsions, makeup bases, essences, But are not limited to, screen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, Do not.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier compounded in a general cosmetic, and examples thereof include oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, Chelating agents, coloring matters, preservatives, perfumes, etc. may be appropriately mixed, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention may vary depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may include animal oils, vegetable oils, waxes, paraffins, starches, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, And the like may be used, but the present invention is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used as a carrier component. In particular, in the case of a spray, , Propellants such as propane / butane or dimethyl ether, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, use is made of an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, But is not limited thereto. These may be used alone or in combination of two or more.

The cosmetic composition of the present invention may preferably be a cosmetic composition for scalp hair care. Specifically, examples include hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, eyebrow hair extender, eyelash hair extender, But is not limited thereto.

The compound of Formula 1 or a cosmetically acceptable salt thereof may be contained in an amount of 0.001 to 50% by weight, preferably 10% by weight based on the total weight of the cosmetic composition.

The present invention provides a health functional food for preventing hair loss or promoting hair growth comprising acetoxycarbic acid acetate or a pharmaceutically acceptable salt thereof as an active ingredient.

[Chemical Formula 1]

The compound of formula (I) of the present invention can be used as a food composition since the compound is derived from a natural product and proved to be stable. The content of the compound of the formula (1) or a pharmaceutically acceptable salt thereof is not limited, but it is preferably 0.001 to 50% by weight, preferably 10% by weight or more of the acetoxychivicol acetate based on the total weight of the health functional food . In addition, the composition may contain additional ingredients which are commonly used in food compositions and which can improve odor, taste, vision and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid and the like. In addition, it may include minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn) and copper (Cu) It may also include amino acids such as lysine, tryptophan, cysteine, valine, and the like. It is also possible to use preservatives such as potassium sorbate, sodium benzoate, salicylic acid and dihydroxyacetate, disinfectants such as bleaching powder and highly bleached sodium hypochlorite, antioxidants such as butylhydroxyanilide (BHA), butylhydroxytoluene (BHT ), Coloring agent (tar color, etc.), coloring agent (such as sodium nitrite, sodium acetylate), bleaching agent (sodium sulfite), seasoning (MSG sodium glutamate, etc.), sweetener (hypodermic, cyclamate, saccharin, (Food additive) such as flavorings (vanillin, lactones, etc.), swelling agents (alum, potassium hydrogen D-tartrate), reinforcing agents, emulsifiers, thickeners, encapsulating agents, gum bases, foam inhibitors, Can be added. The additives are selected according to the type of food and used in an appropriate amount.

The term " pharmaceutically acceptable salt " used in the present invention means a salt which can be used in foodstuffs among salts in which cations and anions are bound by electrostatic attraction, Specific examples include examples of the above-mentioned "pharmaceutically acceptable salts ".

The term " health food "used in the present invention refers to a food having an active health promotion effect or enhancement effect compared to a general food, and a health supplement food means a food for health assistance. In some cases, the terms functional food, health food, and health supplement are used. The food may be prepared in various forms such as tablets, capsules, powders, granules, liquids,

The term " functional food "used in the present invention refers to a food for special health use (FoSHU) It means foods with high effectiveness.

As a specific example of the health functional food of the present invention, it is possible to produce a processed food having improved storage properties while utilizing the characteristics of agricultural products, livestock products or aquatic products by utilizing the compound of formula (1) or a pharmaceutically acceptable salt thereof. The health functional food is not particularly limited, but preferably is margarine, fat continuous or water continuous or bicontinuous spread, fat reduced spread, chocolate, or chocolate coating or chocolate such as confectionery products such as fillings or bakery products, ice cream, ice cream coatings, ice cream containing products, dressings, mayonnaise, cheese, cream substitutes, dried soup, drinks, cereal bars, sauces, snack bars, dairy products, May be used.

The composition for preventing hair loss or comprising hair growth promoting composition containing the compound of formula (I) of the present invention as an active ingredient can be suitably modified to be used for animals other than humans, especially for pets.

The acetoxychivicol acetate of the present invention is excellent in the effect of promoting the growth of hair follicles, so that the effect of preventing hair loss and accelerating hair growth is not only excellent but also is derived from natural products and has no side effects, Composition, quasi-drug composition, cosmetic composition, and health functional food.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a photograph showing the effect of the acetoxychivicacetate of the present invention on hair loss prevention and hair growth promoting effect through animal experiments. Fig.
FIG. 2 is a graph showing the effect of the acetoxychivicol acetate of the present invention on the growth of hair follicles in human hair follicle cell culture. FIG.
Figure 3 is an HPLC chromatogram of a 70% ethanol extract from the root of Alpinia galanga .
FIG. 4 is a photograph and a graph showing the effect of preventing the hair loss and promoting the hair growth of the massive river extract through animal experiments.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Prevention of hair loss and promotion of hair growth of acetoxychicycloacetate

The following animal experiments were carried out in order to confirm the hair loss prevention effect and the hair growth promoting effect of acetoxychivicol acetate. The acetoxychivacoleacetate used in this example was purchased from Abcam.

Seven 7-week-old C57BL / 6 mice were divided into three experimental groups. Testosterone (10 μM solution, 100 μl of foam) was applied to the skin of mice on day 1 in each of the three experimental groups. Group 1 was applied testosterone only, Acetoxychavicacetate (10 μM solution, 50 μl of foam) or minoxidil (2% solution, 50 μl of foam) was applied to the dorsal skin with testosterone on the 3rd day of each group. The experiment was conducted for 17 days based on testosterone application.

As a result of the above test, it was observed that the testosterone-only treated mice progressed to hair loss gradually, and that the testicles had almost no hair on the back skin at about 17 days after the testosterone treatment. On the other hand, in the case of the mouse coated with acetoxychivacaine acetate, hair loss was slightly observed at first, but hair density was significantly higher than that of mice treated with only testosterone, and the concentration of acetoxychivicacetate- On the 14th day, the density of hair increased again. This demonstrates that acetoxycarbicol acetate is effective in preventing hair loss by testosterone and promoting hair growth at the same time (FIG. 1).

In addition, it was confirmed that acetoxychivicol acetate exerts a similar degree of hair loss prevention and hair growth promoting effect as compared with the effect of minoxidil (MNX) currently used as a hair loss treatment agent (Fig. 1). Accordingly, acetoxychivicol acetate is expected to be used in a variety of ways as a treatment for alopecia, because it exhibits similar or better hair loss prevention and hair growth promoting effects than minoxidil, and has no risk of side effects from natural products.

Example 2 Effect of Acetoxy Chivicol Acetate on Follicular Growth Promotion

In order to confirm the hair follicle growth promoting effect of acetoxycarbic acid acetate, the following human hair cell culture organ culture experiment was carried out. The acetoxychivacoleacetate used in this example was purchased from Abcam.

With the consent of the patient who underwent the hair transplant surgery, the hair was separated from the scalp tissue and the lower part of the sebaceous gland was cut to the exact date and the lower part of the hair was used. The tissues were maintained in Williams E medium supplemented with 100 U penicillin / streptomycin, 10 ng / ml hydrocortisone, 10 ug / ml insulin and 2 mM L-glutamine under conditions of 5% CO 2 and 37 ° C, and 500 nM testosterone and each drug Were cultured in medium containing 9 tissues for each condition. The medium is changed every 3 days and after 6 days the growth of the tissue is measured. These human hair follicle cells (n = 36) were divided into four groups. The first group was treated with no drug treatment, the second group was treated with 500 nM testosterone, the third group was treated with 500 nM testosterone, 100 nM of callate acetate and 500 nM of testosterone and 200 nM of acetoxychivicol acetate were administered to the fourth group to measure the length of hair growing in the hair follicle cells.

As a result, in the test group treated with testosterone alone, the length of the hair produced in the hair follicle cells was reduced by about 40% or more, whereas in the test group treated with testosterone and acetoxycholicacetate, all of them were similar to the control group without testosterone treatment (Fig. 2). As shown in Fig. This demonstrates that inhibition of hair growth of hair follicle cells by testosterone is restored by acetoxy chivecoacetate.

Example  3: Acetoxy chi-  Containing a high concentration of acetate Mass river  Preparation of extract

Extracts containing acetoxychivicol acetate at a high concentration were prepared by the following method using a commercially available massive river ( Alpinia galanga, Thailand).

Specifically, after 50 g of a large-diameter tubercle body was cut, 250 ml of 70% water-soluble ethanol was added and ultrasonic extraction was repeated twice for 2 hours. After the extract obtained by the above method was filtered, the filtrate was concentrated under reduced pressure at 40 ° C to 50 ° C and then lyophilized to obtain 2.2g (yield: 4.4%) of 70% ethanol extract having a massive root diameter. The acetoxycarbicycloacetate content in the obtained extract was found to be 14.61% (FIG. 3).

Example 4: Prevention of hair loss and promotion of hair growth of massive river extract

The following animal experiments were carried out using the massive river extract prepared in Example 3.

Two 8-week-old C57BL / 6 mice were divided into two experimental groups, and testosterone (2 mg / ml solution, 100 μl of foam) was applied daily to the skin of the mouse, Was applied only to testosterone, and in the second group, the massive river extract prepared in Example 3 was applied to the back skin with testosterone. The test was conducted for 7 days based on testosterone application. Seven days later, the state of the skin of the hind paw and the size of the hair follicle were measured.

As a result of the above experiment, it was confirmed that when testosterone alone was applied, the hairs of the skin of the mice were almost lost, whereas in the case of the mice coated with the extracts of the massive rivers, hair growth was promoted in the skin of the hind limbs, . In addition, the size of the hair follicle was superior to that of the testosterone alone, and it was confirmed that the extract of massive river containing high concentration of acetoxychivacaine acetate inhibits hair loss and promotes hair growth (FIG. 4).

It is to be understood that both the foregoing general description and the following detailed description of the present invention are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. More variations are possible within a range that does not. Accordingly, the present invention may be embodied in other forms without departing from the spirit or scope of the inventive concept as defined by the appended claims and their equivalents.

Claims (8)

A pharmaceutical composition for preventing hair loss or promoting hair growth, comprising a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof as an active ingredient.
[Chemical Formula 1]

The pharmaceutical composition of claim 1, wherein the composition has an effect of promoting hair follicle growth. A quasi-drug composition for preventing hair loss or promoting hair growth comprising a compound represented by the following formula (1) or a cosmetically acceptable salt thereof as an active ingredient.
[Chemical Formula 1]

4. The quasi-drug composition according to claim 3, wherein the composition has an effect of promoting hair follicle growth. 1. A cosmetic composition for preventing hair loss or promoting hair growth comprising a compound represented by the following formula (1) or a cosmetically acceptable salt thereof as an active ingredient.
[Chemical Formula 1]
6. The cosmetic composition according to claim 5, wherein the composition has an effect of promoting hair follicle growth. A health functional food for preventing hair loss or promoting hair growth, comprising a compound represented by the following formula (1) or a pharmaceutically acceptable salt thereof as an active ingredient.
[Chemical Formula 1]

The health functional food according to claim 7, wherein the health functional food has an effect of promoting hair follicle growth.

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