KR20090053746A - Method and device for promoting hair growth using high-power fractional laser and saline spray method with high temperature - Google Patents

Abstract

The present invention not only stimulates the scalp sufficiently by using a high-power fractional laser beam, but also prevents the laser from reflecting back to the hair, thereby enabling treatment without damage to the hair. In addition, the present invention promotes hair growth by appropriately stimulating the depth of the stem cells of the hair while minimizing damage to the surface of the scalp through a high pressure saline injection method with a high-power fractional laser beam to effectively prevent hair loss. And treatment.

Fractional laser, handpiece, saline solution, scalp, stem cells, hair

Description

TECHNICAL AND DEVICE FOR STIMULATING HAIR USING HIGH POWER FRACTIONAL LASER AND HIGH PRESSURE INJECTION OF WARM NORMAL SALINE OR HAIR PROMOTING AGENTS}

The present invention relates to a method and apparatus for promoting hair growth for use in the prevention and treatment of alopecia.

Hair or hair grows and loses through three hair cycles: anagen, catagen, and telogen. Adults can lose up to about 100 hairs a day, and when more hair falls out, it is called alopecia. In other words, alopecia is a disease in which normally grown hair falls out due to any cause, and is classified according to various factors and symptoms, such as androgenetic alopecia, alopecia areata, telogen effluvium, and the like. There is this.

Among the most representative androgenetic alopecia, genetic predisposition is the main factor, and it is divided into androgenetic alopecia. In male-pattern baldness, male hormones are converted to dihydrotestosterone by catalysis of 5-alpha reductase in humans with a genetic predisposition, and then bind to androgen receptors to breastmilk. Decreased hair follicle function and damage the blood vessels are known to cause hair loss. Androgenetic alopecia is caused by failure to maintain normal hair cycles, shortened growth periods, and changes from terminal hair to vellus hair, and changes in inflammation of the skin tissue around the follicles without the loss of follicles. There is no point. The male type is more prominent in the headline retraction of both temporal heads and hair loss in the head and neck. Female-pattern baldness, on the other hand, is mainly in the form of hair loss, which is mainly preserved in the scalp, but due to the miniaturization rather than destruction of the hair follicles. Hair follicles in the area where the hair growth occurs have increased proportion of resting hair, and the duration of growing period is reduced.

On the other hand, alopecia areata clinically occurs in the scalp or the body of the plaque or confusional hair loss, and round baldness is most common, but in addition to the frontal alopecia in which the entire hair is alopecia or hair loss causing systemic hair loss There may also be alopecia universalis. In alopecia areata, the scalp looks normal, but the hair growth stage ends prematurely and transitions to a resting phase, leading to rapid hair loss. Histological examination shows infiltration of inflammatory cells, such as T cells and macrophages, into monocytes in the peribulbar vessels and outer root sheath, the cause of which has not yet been established. The etiology is reported to work.

Resting alopecia is an increase in the proportion of resting hairs that fall off as part of the growing hair does not meet the growth period and rapidly transitions to resting hair.It is caused by stress, endocrine diseases, nutrition, and drugs. When removed, hair loss decreases as the hair at rest rests normally over several months. However, in many patients, the cause is not found, and hair dropout and subsequent decrease in hair density may be chronic.

Various treatments for treating such alopecia have been developed, but most of the hair loss or wool products currently on the market proved to be effective as quasi-drugs such as cosmetics aimed at improving blood circulation of the skin and nourishing hair roots. Are not things. In addition, there are only two drug treatments recognized by the US Food and Drug Administration (FDA) as minoxidil and propecia, and there is still no treatment method that satisfies alopecia patients.

In addition, methods for promoting hair growth using low-power lasers, which are known to improve blood circulation, have been suggested for treating alopecia (see Korean Patent Application Publication Nos. 2005-18411, 2005-110573, and 2006-34490). However, only the effects of improving the blood circulation and damage to the damaged tissues of the skin have been reported, and there is insufficient scientific evidence on its effect on 5-alpha reductase. In addition, Avram MR et al . "The current role of laser / light sources in the treatment of male and female pattern hair loss", J Cosmet Laser Ther. 2007; 9 (1): 27-8 disclose that low-power laser or light therapy is effective for alopecia and masculinity alopecia, but there is a lack of scientific evidence for this and no studies on the efficacy of precisely designed controls. There is also a lack of assessment of the patient's hair loss condition, the patient's physical condition and the cause of hair loss.

Korean Patent Application Publication No. 2003-92331 discloses hair loss prevention and hair growth promoting hair brush using anion and negative potential, and Korean Utility Model Publication No. 388549 discloses a hair growth promoting comb using a laser. They all use low power lasers to apply the laser directly to the scalp, but low power lasers have limitations in activating hair follicle cells directly.

Meanwhile, the paper [Bernstein EF, "Hair growth induced by diode laser treatment", Dermatol Surg. 2005; 31 (5): 584-6] suggested the hair growth effect using a high power laser, but this was only a phenomenon of accidental hair growth by performing hair removal using a 810 nm diode laser. The research on promoting hair growth has been insufficient. The reason is that when a high power laser is applied directly to the scalp, a large amount of laser may be supplied to a portion of the scalp, which may damage the hair and the scalp cells. Problems can arise.

Similarly, in the case of the professional laser, which is widely used for skin regeneration, laser penetrates into the dermis, amplifies skin tissue activity, creates thousands of micro thermal treatment zones, spreads laser heat to the surrounding area, and intensifies the damaged skin. The effect of reducing normal inflammatory periods with the help of surrounding normal tissues can shorten the duration of treatment, but when applied to the scalp as it is, limitations on inaccuracy, loss of efficiency and damage to the laser fluoroscopy due to light lost and reflected by the hair There is.

Therefore, the hair production or hair loss inhibitory effect is not prominent, but only the research on the low power laser has been in progress for the easy application to the scalp.

Therefore, the present inventors selectively irritate only the hair follicle stem cell region by injecting a high-power laser and a physical wound that is harmless to the human body at a high pressure to warm the saline solution, which can act directly on the hair follicle cells of the skin, compared to a conventional low-power laser. We studied the application of the scalp, and as a result, applying the laser concept to the scalp through a specific type of device prevents the laser reflection on the hair when irradiating a high-powered laser to the scalp. By activating the hair follicle cells and scalp cells by the delivery and improving the blood circulation of the scalp, and found that the high pressure warm saline and hair spraying method can promote hair growth harmless to the human body and completed the present invention.

It is an object of the present invention to provide a hair growth promoting method and apparatus that can be usefully used for the prevention and treatment of alopecia by sufficiently stimulating the scalp without damaging the scalp by using a high power facial laser of hair.

In addition, the object of the present invention by using a high-power fractional laser device and a high pressure pressure to spray the warm physiological saline and hair regrowth components to directly stimulate the dermal papilla cells and hair stem cells that play an important role in hair formation The present invention provides a method and apparatus for promoting hair growth by maximizing the effect of hair growth by efficiently stimulating the location of the hair follicles (lower dermis or upper subcutaneous fat layer) along with thermal stimulation caused by high power laser.

An apparatus for promoting hair growth using a facial laser beam according to the present invention for realizing the above object comprises: a laser generator for generating a laser beam; A beam delivery line connected to the laser generator and transmitting a laser beam; It characterized in that it comprises a handpiece made to irradiate the laser to the scalp while moving the laser beam transmitted from the beam transmission line to the scalp to be treated in a rolling manner (rolling).

The handpiece may comprise a coupling part for connection with the beam transmission line, a scanner for scanning a laser beam passing through the coupling part, and a straightening or focusing method for radiating the laser beam scanned by the scanner. It is preferably configured to include a lens, and a beam irradiator for irradiating a laser beam passing through the lens to the treatment site.

The handpiece may include a hand body configured to be held by an operator and a rolling body rotatably connected to the hand body through a connecting member, and the coupling body, the scanner, the lens, and the beam irradiation unit may be connected to the rolling body. It is preferred to be provided.

At this time, the rolling body has a cylindrical member so that the coupling, the scanner, the lens of the hand body is supported and installed so that the laser beam is introduced while connecting both connecting members, and the cylindrical structure is supported and rotated by the center member. It is preferable that it is composed of a rotating body provided with a projection irradiating portion consisting of a plurality of projection structures arranged at regular intervals.

The projection unit of the rotor may be coated with a reflective material on an inner surface to which the laser is irradiated or an outer surface to which the laser is emitted, so that photon recycling may be performed.

Hair growth promoting device using a saline solution or a chemical solution according to the present invention for realizing the above object, the hand body is provided with a saline storage space in which the saline solution or the chemical solution is stored, the pressing means provided inside the hand body, and And a piston for pressurizing the saline solution or the chemical solution stored in the saline reservoir while moving forward by the pressurizing means, and an injection nozzle provided at the front of the hand body to inject the saline solution or the chemical solution stored in the saline reservoir.

Preferably, the pressurizing means is a high pressure gas provided inside the hand body.

In addition, the saline reservoir may be configured so that the saline can be inserted.

In addition, the inside of the hand body is preferably provided with a temperature control means to adjust the temperature of the saline around the saline storage space.

In the hair growth promoting apparatus using the high power fractional laser and the saline solution or the chemical solution according to the present invention for realizing the above problems, a laser beam irradiation unit for irradiating a fractional laser beam to the epidermal layer of the scalp is located at the center, and the laser beam irradiation unit The circumferential portion is characterized in that the saline injection unit for injecting a saline solution or a chemical solution so that the wound on the dermis or subcutaneous fat layer of the scalp is arranged.

A method for promoting hair growth using a facial laser beam according to the present invention for realizing the above object is characterized by irradiating a high power fractional laser beam to the scalp.

In addition, the hair growth promoting method using a laser laser beam according to the present invention for realizing the above object comprises the steps of splitting the hair remaining in the bald area of the patient with a tool or hand in the form of a balma; After the above step, the handpiece of the laser irradiation apparatus of the roller type is in close contact with the hair loss site to be treated, and characterized in that it comprises a step of irradiating the hair loss site with a high power fractional laser beam while gently reciprocating.

The high power fractional laser has a wavelength of 400 to 12,000 nm and an energy per area of 0.001 to 100,000 J / cm 2; It is preferable that the energy per wave is 150 mJ or less, and the wave time is 100 msec (millisec) or less.

It is preferable that the energy range of the high power principal laser is 5-10 mJ per beam and the density is 100 to 1024 pieces / cm 2.

Preferably, the high power fractional laser stimulates an area of 10 to 4,000 μm deep and 10 to 1,000 μm diameter of the scalp.

According to an aspect of the present invention, there is provided a method of promoting hair growth using a facial laser beam and a saline solution or a chemical solution, the method comprising: making a thermal stimulus using a laser beam on the surface layer of a scalp using a high power fractional laser; Simultaneously with or before and after the above step, spraying the saline solution or the medicinal solution with a strong pressure so that a wound can be formed at an easy depth of stimulation of the hair stem cells.

Herein, the spraying temperature of the saline solution or the chemical solution is preferably sprayed at about 37 ° C to 42 ° C.

In addition, the chemical solution, it is preferable to use a saline solution or a mixed chemical solution in which at least one of a peptide, growth factor, finasteride, vitamin, minoxidil, capsaicin is mixed.

Hair growth promoting method using a saline solution or a chemical solution according to the present invention for realizing the above-mentioned problem is characterized in that by spraying a high pressure saline solution or a chemical solution to the scalp to damage a certain depth of the scalp to promote hair growth by stimulation of hair stem cells It is done.

According to the hair growth promoting method according to the present invention, by preventing the reflection of the high-power laser to the hair to deliver sufficient energy to the scalp without damaging the hair to promote the secretion of useful growth factors, regulate the hair cycle, hair papilla cells and keratin It is also possible to inactivate hair-forming cells, such as hair-forming cells, evenly, and indirect hair growth due to heat energy of laser and increased blood flow generated during wound restoration. In addition, high pressure saline injection is applied to the hair stem cell area to induce damage to the microdermal layer, and in the process of restoring the damage, hair stem cells are activated to promote hair growth and various types of alopecia can be effectively prevented and treated. have.

Hereinafter, exemplary embodiments of the present invention will be described with reference to the accompanying drawings.

The hair growth promoting method of the present invention irradiates the scalp with a high-power fractional laser of various wavelengths to induce sufficient stimulation on the scalp to promote hair growth.

In addition, the present invention can be sprayed on the scalp by densely spraying high-pressure warm saline or hair regrowth component with a high-power fractional laser to promote hair growth by causing sufficient irritation to the scalp.

In another aspect, the present invention by densely spraying the high pressure warm saline or hair regrowth component to the scalp to cause sufficient irritation to the scalp to promote hair growth.

Referring to the main features of this invention are as follows.

First, a hair growth promoting method using a high-power proxy laser will be described.

The procedure for promoting hair growth using a high power surgical laser may be performed by combing the patient's hair loss area without special anesthesia, or by using a tool such as a laser handpiece 3 to be described below, or by using the operator or assistant's hand. The hair remaining at the bald area is cut (folded) on both sides in the form of a garma.

Thereafter, the handpiece of the laser irradiation apparatus of the roller type to be described in detail below is in close contact with the hair loss site to be treated, and the high power fractional laser beam is irradiated to the hair loss site while gently reciprocating.

The high power fractional laser, irradiation method, and laser irradiation apparatus used in the procedure for promoting hair growth will be described.

First, a high-power surgical laser and a method of irradiating this laser beam will be described.

"High power" means variables of the laser beam that raise the temperature of the scalp area to be treated to a level at which tissue reactions occur (e.g., energy per area, power, timing, pulse duration, Imply a range of values of interpulse duration, wavelength, etc.).

The laser-stimulated scalp may be divided into a site (A), a 62 to 75 ° C site (B), a 42 to 62 ° C site (C), a 45 ° C site (D), and the like, which reach at least 75 ° C depending on the laser irradiation conditions. The more the laser penetrates into the scalp tissue, the higher the scalp temperature and the more active the tissue response. C or D sites at somewhat lower temperatures may not cause tissue reactions. In other words, in order to cause a reaction in the scalp tissue, the scalp temperature must be raised above an appropriate temperature, and for this purpose, high power laser irradiation is required.

Such high power lasers capable of inducing sufficient irritation to the scalp have a wavelength of 400 to 12,000 nm, preferably 500 to 3,000 nm, more preferably 1,000 to 2,000 nm, even more preferably 1,400 to 1,600 nm; The energy per unit area is 0.001 to 100,000 J / cm 2, preferably 1 to 1,000 J / cm 2; The energy per wave is 150 mJ or less, preferably 50 mJ or less; The wave time is 100 msec (milli sec) or less, preferably 50 msec or less, and more preferably 0.4 to 10 msec.

When the scalp is stimulated with a high power laser, the stimulus reaches the epidermis and dermis of the scalp skin, and the irritated scalp area is 10 to 4,000 μm in depth, 10 to 1,000 μm in diameter, preferably 25 to 750 μm, more Preferably it is 50-500 micrometers, and it will have various shapes including round pillar shape.

In addition, the pitch between dots, which is the irradiation site of the proxy laser, is important in the high-power fractional laser treatment according to the present invention. The dot pitch determines the density of the laser beam per certain area, for example per square centimeter. In other words, when the distance of galvanometer is 1 cm, when the distance of each laser beam irradiation part (dot) is 1mm maximum, the density starts from the minimum value of 100 / cm 2, If the minimum value is 312 µm, 32 lines in a straight line of 1 cm, that is, the maximum density is about 1024 pieces / cm 2. Therefore, the density of the high-power surgical laser suitable in the present invention can be set in the range of 100 to 1024 (number of heat treatment zones) / ㎠, if the scalp to increase the density by allowing the reaction to heat, such as repeated irradiation Enhance biological tissue response.

The high power fractional laser uses the principle of irradiating about 100 to 1024 lasers (micro thermal zones) at a size of 1 cm 2 to quickly regenerate the treatment area by the skin tissue of the healthy scalp that is not irradiated.

Next, a high-power proxy laser irradiation apparatus will be described.

The laser irradiation apparatus according to the present invention uses a high power fractional laser device including a handpiece operated in a rolling manner to irradiate a high power fractional laser to the scalp.

Such a laser irradiation apparatus will be described with reference to the drawings.

The laser irradiation apparatus of the present invention, as schematically shown in Fig. 1, comprises a laser generator 1 for generating a laser beam, a beam transmission line 2 for transmitting a laser beam connected to the laser generator, It consists of a handpiece (3) adapted to irradiate the scalp with the laser beam transmitted from the beam delivery line (2).

Here, the laser generator 1 may use a conventional device for generating a high power laser, which emits photons at the atoms of the laser light source by changing the laser light source emitting a laser light, the laser light source into a high energy state. A laser stimulus source, a reflector which causes these photons to strike the stimulated atoms of the laser light source again to emit laser light.

Light sources for high power lasers include diode lasers, diode pumped solid state lasers, Er (erbium): YAG (yttrium aluminum garnet) lasers, Nd (neodymium): YAG lasers, Er: glass lasers, argon ion lasers, helium-neon lasers, Carbon dioxide lasers, excimer lasers, fiber lasers, ruby lasers, frequency multiplied lasers, and the like, but are not limited thereto. Among them, the Er: glass laser having a laser wavelength of 1540 to 1550 nm is most preferred.

The beam transmission line 2 is for transmitting the laser beam emitted from the laser generator 1 to the handpiece 3, but preferably composed of optical fibers, but is necessarily limited to optical fibers. If it is not a material that serves as a passage that can deliver the laser beam can be adopted by various configurations. In addition, even if composed of optical fibers, it is a matter of course that the coating structure for protecting the optical fibers can be installed and configured.

The handpiece 3 will now be described in detail.

The handpiece 3 is a device for hand-held treatment by a practitioner to irradiate the surgical laser on the scalp in a state of rolling contact with the portion of the scalp to be treated.

The basic structure of the handpiece 3 includes a coupling part 4 for connection with the beam delivery line 2, a scanner 5 for scanning a laser beam passing through the coupling part 4, and It consists of a lens 6 for straightening or focusing to emit the laser beam scanned by the scanner 5, and a beam irradiator 7 for irradiating the laser beam passing through the lens 6 to the treatment area.

In this case, instead of the scanner 5, a microlens array for dividing a single laser beam into small beams forming a plurality of arrays may be used to control the arrangement of the laser beams. The beam irradiator 7 may be formed in a protrusion structure for irradiating a laser beam to a desired target portion.

The handpiece 3 having such a basic structure can be designed and manufactured as shown in FIG. 2, and a specific embodiment of the handpiece 3 will be described with reference to FIG. 2.

Since the handpiece 3 of the present invention is basically configured to irradiate the laser with the roller while contacting the scalp in a roller manner, the hand body 10 and the connecting member 15 to the hand body 10 are large. Rolling body 20 is rotatably connected through.

The hand body 10 is connected to the laser generator 1 through a beam delivery line 2 made of optical fibers or the like, which has been described with reference to FIG. 1, wherein the operator has an operator. The handle is configured to hold by hand. Of course, the size of the hand body 10 may also be configured to produce a size that the operator can grab by hand. In addition, the hand body 10 may be provided with various operation switches required for the procedure, or may be configured with a display unit for displaying the output range and output state of the laser.

The connecting member 15 is a portion configured to support the rolling body 20 to protrude forward in a state supported by both sides of the hand body 10, the beam transmission line for transmitting a laser beam therein ( 2) is preferably installed and configured.

The rolling body 20 has a center member 21 for introducing a laser beam while connecting the two connecting members 15, and a rotating body having a cylindrical structure to be supported and rotated by the center member 21 ( 25). Referring to FIG. 3, the coupling member 4, the scanner 5, the lens 6, and the like of the hand body 10 are supported and installed on the center member 21. In addition, the beam guide 23 is also supported and installed.

The rotating body 25 is formed with a projection type irradiation part 7 composed of a plurality of projections arranged at regular intervals.

Accordingly, the laser beam transmitted through the center member 21 through the hand body 10 and the connecting member 15 passes through the coupling part 4, the scanner 5, and the lens 6 while being proximal. It is emitted to the outside through the protruding irradiation part 7 of the rotating body 25 in the state differentiated by the laser. At this time, the rotating body 25 rotates in contact with the user's head. When the rotating body 25 coincides with the laser beam emitted through the lens 6 of the rotating projection unit 7, the rotating body 25 is emitted to the outside while being treated to the treatment part. Can be investigated.

Here, the rotational position of the rotating body 25 may be grasped so that the laser beam may be emitted only when the front part of the lens 6 and the projection type irradiation part 7 coincide with each other.

On the other hand, the protruding projection 7 may be configured to take a protruding form as shown in the figure, or may be formed in a protruding comb shape. The projection type irradiation part 7 may be made of a material such as glass, plastic, metal, etc., and is formed on the outer surface of the rotating body 25 to irradiate a laser beam by contacting the scalp when the rotating body 25 rotates. . In this case, the laser beam may be irradiated as it is in contact with the scalp, or the laser beam may be reflected from the inside using the principle of photon recycling, and then irradiated with the scalp again. In order to achieve the internal reflection of the laser, the inner surface of the projection part 7 may be coated with a reflective metal material (eg, copper, silver, gold, etc.). In addition, when the outer surface of the projection type irradiation part 7 is coated with a reflective metal material, even if the irradiated laser is reflected or scattered from the hair, it may be reflected back to the scalp from the outer surface of the projection type irradiation part 7.

Various embodiments of the configuration of such a projection type irradiation part 7 will be described with reference to FIGS. 4 to 6.

The projection type projecting portion 7 shown in FIG. 4 has a basic laser beam radiation path structure, and is irradiated through the scanner 5 and the lens 6 provided in the beam guide 23 of the handpiece 3. The laser beam is constructed so that it can be irradiated directly to the desired site of the scalp after it is emitted straight away.

As shown in FIG. 5, the protruding irradiator 7 ′ is coated with a reflective metal material on the inner surface of the protruding irradiator 7 shown in FIG. 4 so that the beam is reflected inside the passage on the principle of photon recycling and then back to the scalp. It is configured to be investigated.

The projection irradiated portion 7 ″ shown in FIG. 6 is coated with a reflective metal material on the outer surface of the laser beam emitting surface so that the laser beam, which has been emitted to the outside, is reflected back from the coated outer surface even if the laser beam is reflected or scattered from the hair. The projection irradiated portion 7 "prevents damage to the handpiece 3 and hair damage caused by the laser beam reflected from the hair as well as the efficiency and energy saving of the power supplied to the light source. can do.

Now, the results of experimenting with the actual hair growth promotion using the high-power fractional laser irradiation apparatus as described above will be described by way of example. Experimental results to be described below are not limited thereto but merely to exemplify specific experimental results.

Test 1: Activation of hair follicle cells according to scalp treatment of high power fractional laser

In order to confirm whether the hair follicle cells are activated by the scalp treatment of the high power facial laser according to the present invention, laser irradiation on the scalp of the alopecia patient was performed using the high power facial laser irradiation apparatus described with reference to FIG. 1. . At this time, Er: glass laser having a wavelength of 1540 to 1550 nm was used as the laser light source.

After laser treatment, dermal papilla cells (DPCs) were isolated from test patients through tissue biopsies, and the RNA levels of genes involved in the hair cycle after 6 hours, 12 hours, and 24 hours, respectively, were measured using RT-PCR (real time polymerase chain). reaction (real-time polymerization chain reaction), and the results are shown in FIGS. 7 and 8.

7 and 8, the expression of wnt5a and beta-catenin, which are factors of the Wnt signaling pathway involved in the phase transition from the resting phase to the growth, increased by three-fold, respectively. In addition, PDGF-A (platelet-derived) Growth factor) and KGF (keratinocyte growth factor) expression was increased, which means that the stimulation of the scalp using the high-power fractional laser irradiation apparatus according to the present invention is performed through the cycle transformation during tissue restoration of the dermal papilla cells. It may be able to act on hair regrowth.

In addition, the expression pattern of protein Akt and its activated form, p-Akt (phosphorylated form), was evaluated by Western blot to confirm the phenomenon of inducing proliferation of the isolated papilla cells. The results are shown in FIGS. 9 and 10.

9 and 10, it can be seen that the phosphorylated p-Akt increases by approximately two-fold over time in the dermal papilla cells, indicating that high-power fractional laser treatment induces cell proliferation through the Akt pathway.

Test 2: Test of Hair Growth Effect in Mice Following Treatment with High Power Fractional Laser

Two steps of experiments were performed to measure the hair growth effect of the high power fractional laser treatment.

First, as a first experiment, 10 dorsal 6-week-old Spraue-Dawely white mice were removed with back hairs, and 5 skins with clear back skin were selected and irradiated with a high power laser in the same manner as in Example 1. After 7 and 14 days after the laser irradiation, after taking pictures to evaluate the length of hair and the degree of growth, the results for each mouse is shown in Figures 11 to 15.

From FIG. 11 to FIG. 15, after 7 days, the area irradiated with the laser generates more hair and grows faster than the unirradiated area (control), and after 14 days, the site irradiated during the wound healing process by laser stimulation. It can be seen that hair growth has expanded to the periphery.

On the 14th day, 10 random hairs of the irradiated site were selected as the test group, 10 random hairs of the unirradiated site were selected as the control group, and the length thereof was measured by an optical microscope. Is shown in Table 1.

Hair growth comparison between control and experimental groups (n = 10) Control Test group Hair growth (mean ± standard deviation: mm) 2.01 ± 0.52 4.50 ± 0.77

From Table 1, it can be seen that the test group increased the hair growth rate more than two times compared to the control.

In addition, 10 white mice were selected as the second experiment, and the lasers were irradiated with different energy (mJ) and density of the laser (dog / cm 2).

FIG. 16 is a photograph of hair growth results of a mouse irradiated with two lasers at a high energy of 15 mJ and a low density of 400 cells / cm 2, and it can be seen that hairs are concentrated in the center of the irradiation site.

17 is a photograph of the hair growth results of a mouse irradiated with two lasers at a low energy of 7 mJ and a high density of 800 cells / cm 2, and it can be seen that the hair growth is superior to that of FIG. 16.

The results in FIGS. 16 and 17 show that the hair growth may vary depending on the laser energy and the density of the laser.

In addition, as a third experiment, the hair growth results were tested after irradiating a laser to each mouse by varying the energy (mJ) and the density of the laser (spot / cm 2).

FIG. 18 is irradiated with laser energy equal to (a) and (b) at 4 mJ, and a laser beam having a beam size of 150 μm is used at a density of (a) 500 spot / cm 2 and (b) 1000 spot / cm 2. The results of each investigation were tested.

19 is irradiated with laser energy of (a), (b) equally 6 mJ than in FIG. 18, and a laser beam having a beam size of 150 μm is (a) 500 spot / cm 2, (b) 1000 The test results were taken with the density of spot / cm 2.

20 is irradiated with laser energy of (a) and (b) to further increase to 8 mJ, and one laser beam having a beam size of 150 μm is (a) 500 spot / cm 2 and (b) is 1000 spot / The test results were taken with the density of 2 cm 2, respectively.

As can be seen from the test results of FIGS. 18 to 20, when the laser energy is 6 mJ or more and a stimulus density of 1000 spot / cm 2 or more is applied, sufficient thermal stimulation is performed to further promote hair growth.

In the present invention as described above has been described with respect to the embodiments using only a high-power professional laser. Hereinafter, a method and a device used to promote hair growth by injecting a high pressure warm saline or a hair repellent component densely injected into the scalp together with the high-power fractional laser as described above to induce sufficient stimulation to the scalp.

The procedure using a high power fractional laser and a high pressure warm saline, as described above, uses a high power fractional laser to create thermal stimuli using a laser beam on the surface of the scalp.

Thereafter, the saline solution is sprayed to the hair loss site with a strong pressure, in which the injection of the stimulation of the hair stem cells is possible to form a wound that is irritated within and around 1 mm deep of the skin. Here, it is also possible to spray a drug such as a hair regrowth instead of saline.

That is, it is also possible to use only saline, and it is also possible to inject and use components such as peptides, growth factors, finasteride, vitamins, minoxidil, capsaicin and the like in saline. It is also possible to use only drugs (medicinal solutions) containing ingredients such as peptides, growth factors, finasterides, vitamins, minoxidil and capsaicin.

In this method using high-power fractional laser and high-pressure warm saline, the surface of the scalp is thermally stimulated by the high-power fractional laser, and at the same time, the deep layer of the scalp is irritated by spraying saline (including a hair regrowth agent) to form hair wounds. It is configured to promote. At this time, the laser irradiation and the saline injection is preferably performed at the same time, but may be made by a method of performing one (for example, high-power practitioner laser irradiation) first, and then another one (for example, saline injection) with a time difference. have. In addition, hair growth promotion may be performed using only a saline injection method without irradiating a high power surgical laser.

Here, the injection of physiological saline using high pressure is difficult to make a direct wound to the deep part of the scalp by using a high energy of a certain amount when irradiating a high power fractional laser to the scalp, causing severe damage to the normal hair. As a supplementary method, if a certain amount of saline is sprayed at a high pressure by using a device capable of high pressure injection in a certain area at a time, the wound is made as deep as the part where the sebaceous glands where stem cells of hair follicles are connected is connected. The recovery process can promote hair growth.

These wounds secrete growth factors such as IGF, HGF, and PDGF during the recovery process, affecting the hair cycle and activating hair stem cells as in Cotsalelis et al.'S study to induce the formation of new hair follicles. Here, Cotsalelis et al. Reported that wounding in animal models allows the formation of new hair follicles through the release of various hair growth factors and regulators. [Cotsalelis et al. Nature 2007 may]

The saline injection device used together with the saline injection device and the laser irradiation device used in the procedure for promoting hair growth as described above will be described in order.

21 and 22 are views showing a saline injection device according to the present invention.

As shown in FIG. 21, the saline injection device 30 may have a structure such as a liquid injection gun. Basically, when the lever 32 is pulled, the saline solution may be injected through the injection cap 50 having a plurality of injection holes. It is configured to be sprayed.

In FIG. 21, reference numeral 33 denotes a heat fluid supply unit for temperature control of the saline solution, and 34 denotes a line for supplying a high pressure gas.

With reference to FIG. 22, the internal structure of a saline injection device is demonstrated.

The saline injection device 30 as shown is configured such that the saline solution is injected by applying a pressure to the saline storage space 46 by the piston 43 moved by the air pressure (gas pressure) provided from the outside.

That is, the gas pressurization space 42 connected to the line 34 for supplying gas in FIG. 21 is located inside the hand body 41. At this time, the supply pressure of the gas is preferably configured to be determined by the degree of pulling the injection lever 32 shown in FIG. One side of the gas pressurization space 42 may have a small hole 42a through which gas is discharged.

The piston 43 is located in front of the gas pressurization space 42, the piston 43 is to provide the pressure for the saline injection while advancing in accordance with the pressurized state of the gas inside the hand body (41). The piston 43 may be formed in a structure in which the piston 45 at the gas pressurizing space 42 and the piston 44 at the saline storage space 46 are connected in two stages.

The saline reservoir 46 is located in front of the piston 43. The saline storage space 46 may be configured by directly filling and storing saline solution and inserting the saline bottle 47 into the saline storage space 46.

In the case where the saline bottle 47 is configured here, when the saline bottle 47 is inserted into the hand body 41 while the front piston 44 portion of the piston 43 is already inserted in the rear side, It can be configured in such a way that is automatically coupled with the piston 45 inserted into the gas pressurization space (42). Such a saline bottle 47 can be configured to be mounted by opening the injection cap 50, the injection hole is formed.

The injection cap 50 is formed with a saline injection hole, and can be configured as a single structure or a multiple structure. In the case of the multiple structure, at least 10 saline solution per unit area (1 cm) is preferably configured to enable high-pressure injection at a pressure that can damage a certain depth of the scalp. At this time, for example, it can be configured so that about 30 saline spray stems can be made in an area of 3 ㅧ 3cm.

Meanwhile, a means 48 for adjusting the temperature of the saline solution may be installed around the saline storage space 46 or the saline bottle 47, in which a coil type electric heater is installed or an external heat fluid is passed through. Can be configured.

FIG. 21 shows an embodiment in which hot steam is supplied to the outside of the saline bottle 47, which is an area in which the means 48 for controlling the temperature is installed in connection with an external warmer.

The heater installation method may be configured by installing a heating coil in the vicinity of the saline bottle 47 installation portion, as indicated by the dotted line of FIG.

On the other hand, in order to change the gas filling pressure of the gas pressurizing space 42, by changing the position of the drug container in the spring or screw 49 adjustment method on the front of the hand body 41, the piston 43 is the saline bottle The pressure applied to the 47 or the saline reservoir 46 may be adjusted.

In addition, while the above-described method of adjusting the pressure of the saline solution by pulling the injection lever 32, it is also possible to configure a constant pressure injection method so that the saline solution is injected only at a constant pressure.

In the saline injection device 30 configured as described above, when the injection lever 32 is pulled, a gas pressure is applied to the gas storage space 42, and the piston 43 is advanced by this pressure, and thus the saline solution 47 is applied. It is possible to spray the saline solution stored in.

Now, the saline injection device configured with the laser irradiation device will be described with reference to FIG.

FIG. 23 is a schematic diagram illustrating a state in which a laser and a saline are injected into a treatment part by using a laser irradiation and a saline injection device.

As shown in the figure, the laser irradiation and saline injector 60 has a laser beam irradiator 61 for irradiating a fractional laser beam at a central portion thereof, and injects saline to the circumferential portion of the laser beam irradiator 61. A plurality of saline injection parts 65 may be arranged.

The arrangement relationship between the laser beam irradiator 61 and the saline injection unit 65 may be changed in various ways according to the implementation conditions, and the present invention is not limited to those illustrated in the drawings.

However, the laser irradiation position of the laser beam irradiator 61 and the saline injection position of the saline injection unit 65 are preferably arranged so as to stimulate the epidermal layer and subcutaneous fat layer on the scalp.

The laser beam irradiator 61 may be configured using a variety of known fractional laser irradiators as long as the structure can irradiate the epidermal layer to the epidermal layer, and the saline spray unit 65 has been described with reference to FIG. 22. It can be configured to have an internal structure. However, for simultaneous operation (selective operation is also possible) of the saline injection unit 65 and the laser beam irradiation unit 61, it is preferable to configure the laser beam irradiation by an electronic control method, and to configure the saline injection control. In addition, the saline injection unit 65 may be configured to adjust the angle with respect to the laser beam irradiator (61).

In FIG. 23, reference numeral 62 denotes a transmission mirror from which a proxy laser is emitted, and 63 denotes a holder for integrally connecting the saline injection unit 65 to the body of the laser beam irradiator 61.

The laser beam irradiating and saline spraying device 60 as described above, irradiates a facial laser beam to the surface layer of the scalp through the laser beam irradiating portion 61 positioned in the center to make thermal stimulation, and the saline spraying portion located around ( 65), a strong pressure is applied to the saline solution (or a drug such as a hair restorer) to stimulate the skin dermis or subcutaneous fat layer, which is easy to stimulate hair stem cells, at the site of hair loss, thereby creating a wound.

At this time, when spraying the saline solution to the scalp, by adjusting the spray angle or direction, the spray angle is adjusted to stimulate the skin dermis layer or subcutaneous fat layer of about 1 mm deep, that is, 0.5 ~ 3mm deep of the skin, and also the saline spray temperature It is preferable to spray by warming about 37 degreeC-42 degreeC.

Now, the results of the test of the actual hair growth promotion using the laser irradiation and the saline injector as described above will be described by way of example.

Test 4: Alopecia Effect in Alopecia Patients by High Power Fractional Laser and Saline Injection

In order to confirm the hair growth effect and the hair loss prevention effect by the high power laser irradiation, the following test was performed.

First, a total of 40 patients with androgen alopecia (Hamilton-North taxonomy II and above) and 20 patients with alopecia areata (other patients who have not responded to conventional or topical therapy or immunotherapy for at least 3 months) Ten test subjects consisted of each test group and control group. The test group was treated with a high-power fractional laser and a high pressure warm physiological saline injection method in the same manner as in Example 1 at least six times at two-week intervals, and the control group received low-power laser irradiation and minoxidil.

Table 2 shows the results of the phototrichogram analysis for the test group and the control group.

From Table 2, it can be seen that the test group shows a marked improvement in hair loss compared to the control group.

In addition, the upper head of the test group was photographed and shown in FIG. 24 (male androgenetic alopecia patient), FIG. 25 (female androgenetic alopecia patient), and FIG. 26 (circular alopecia patient) (left photograph: before treatment, right side). Photograph: after the treatment), as a result it can be confirmed that hair growth was promoted by the high-power fractional laser and high-pressure saline injection therapy according to the present invention.

As described above, when irradiating a high power fractional laser and simultaneously spraying saline solution to treat hair loss, thermal irritation is applied to the surface layer of the scalp using a laser beam, and a high pressure saline solution is sprayed to damage a certain depth of the scalp. By stimulating the epidermal layer of the scalp and hair stem cells at the same time, thereby promoting hair growth.

1 is a configuration diagram schematically showing a high power fractional laser irradiation apparatus according to the present invention,

2 is a schematic perspective view of a handpiece according to the present invention;

3 is an internal configuration diagram of the handpiece shown in FIG.

4 to 6 are views of various embodiments of the projection of the handpiece according to the present invention,

7 and 8 are a view showing the results of RT-PCR for observing whether the hair follicle cell activation of the scalp after high power fractional laser irradiation according to the present invention,

9 and 10 are Western blotting results for observing whether hair follicle cells are activated in the scalp after high power surgical laser irradiation according to the present invention;

11 to 15 are pictures showing the mouse hair growth photographs on days 7 and 14 after high-power fractional laser irradiation according to the present invention, respectively;

16 and 20 are photographs showing the degree of hair growth according to the laser energy and density of the laser in accordance with the present invention,

21 is a configuration diagram showing a saline injector according to the present invention,

22 is an internal configuration diagram of the main part of the saline injection device according to the invention,

23 is a block diagram showing a laser irradiation and saline injection device according to the invention,

24 to 26 are diagrams showing the state of hair before and after high power fractional laser irradiation for alopecia patients.

Claims (11)

A laser generator for generating a laser beam; A beam delivery line connected to the laser generator and transmitting a laser beam; Using a facial laser beam comprising a handpiece configured to irradiate a surgical laser onto the scalp while moving the laser beam transmitted from the beam delivery line in a rolling manner to the scalp portion to be treated. Hair growth promoting device. The method according to claim 1, The handpiece, A coupling portion for connection with the beam delivery line, a scanner for scanning a laser beam passing through the coupling portion, a lens for straightening or focusing to emit a laser beam scanned by the scanner, and the lens Hair growth promoting device using a laser beam, characterized in that it comprises a beam irradiator for irradiating the laser beam passed to the treatment site. The method according to claim 2, The handpiece, A hand body configured to be held by an operator, and a rolling body rotatably connected to the hand body through a connecting member, And a coupling part, a scanner, a lens, and a beam irradiator on the rolling body. The method according to claim 3, The rolling body, Couplings, scanners, and lenses of the hand body are installed to support the laser beam while connecting the two connecting members, and a plurality of cylindrical members arranged at regular intervals to have a cylindrical structure so as to be supported and rotated by the center member. Hair growth promoting device using a laser beam, characterized in that consisting of a rotating body provided with a projection irradiating portion made of a projection structure of the. The method according to claim 5, The projection type irradiator of the rotating body is coated with a reflective material on the inner surface to which the laser is irradiated or the outer laser is emitted hair growth promoting apparatus using a laser beam characterized in that the photon recycling is configured to be made. A hand body having a saline storage space for storing saline or chemicals; Pressing means provided on the inside of the hand body, A piston for pressurizing the saline solution or the chemical liquid stored in the saline storage space while moving forward by the pressurizing means; Hair growth promoting device using a saline solution or a chemical solution, characterized in that it comprises a spray nozzle which is provided on the front of the hand body so that the saline solution or the chemical solution stored in the saline storage space. The method according to claim 6, The pressurizing means is a hair growth promoting device using a saline solution or a chemical solution, characterized in that the high-pressure gas provided inside the hand body. The method according to claim 6, The saline storage space is a saline solution or hair growth promoting device, characterized in that the saline solution is configured to be inserted. The method according to claim 6, The inside of the hand body hair growth promoting device using a saline solution or a chemical solution characterized in that the temperature control means is provided to adjust the temperature of the saline around the saline storage space. A laser beam irradiator for irradiating a fractional laser beam to the epidermal layer of the scalp is located at the center, and a saline jet unit for discharging saline or a chemical solution is formed at the periphery of the laser beam irradiator to make a wound on the dermis layer or subcutaneous fat layer of the scalp. Hair growth promoting device using a high power fractional laser and saline or a chemical solution, characterized in that the configuration. The method according to claim 10, The saline injection unit, A hand body having a saline storage space for storing saline or a chemical solution, a pressurizing means provided inside the hand body, a piston for pressing the saline solution or a chemical solution stored in the saline storage space while moving forward by the pressurizing means; The high power fractional laser and the saline solution or hair growth promoting device, characterized in that it comprises a spray nozzle which is provided on the front of the hand body so that the saline solution or the chemical solution stored in the saline storage space.

Images