KR20080093500A - Roe deer urine extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging, and more particularly, to a cosmetic composition containing a roe deer urine extract to prevent skin aging. According to the present invention, the roe deer urine extract promotes the proliferation of skin cells, inhibits the synthesis and synthesis of fibroblast collagen synthesis, enhances the expression of integrin and exerts an excellent antioxidant effect to improve skin wrinkles and enhance skin elasticity. Prevents skin aging.

Description

Cosmetic composition for preventing skin aging containing roe deer urine extract {COSMETIC COMPOSITIONS FOR PREVENTING SKIN AGING COMPRISING EXTRACT ASTILBE CHINENSIS VAR. DAVIDII}

The present invention relates to a cosmetic composition for preventing skin aging containing roe deer urinary tract extract, and more particularly, containing roe deer urine extract to promote the proliferation of skin cells, to inhibit the synthesis and synthesis of collagen of fibroblasts, It relates to a cosmetic composition that enhances the expression, has an excellent antioxidant effect and effectively prevents skin aging through skin wrinkle improvement and skin elasticity improvement.

The skin is the largest organ of the human body, occupying about 16% of the total body volume, and an important protective barrier that protects the body from many deadly harmful factors, such as temperature, humidity, and ultraviolet radiation, which are in direct contact with the external environment and are likely to enter the body. Play a role. However, as they age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization.

Skin aging is largely divided into intrinsic aging and extrinsic aging (Cosmetics & Toiletories, 111: 31-37 (1996)). Endogenous aging is a aging phenomenon that occurs naturally as the physiological function of the skin gradually decreases with age. Clinically, elasticity is reduced, skin texture is rough, deep wrinkles are formed, and pigments are deposited. Dermatol., 130: 87-95 (1994). Exogenous aging refers to the aging phenomenon caused by exogenous factors such as ultraviolet light, reactive oxygen species and stress. These factors lead to rapid aging of the skin, resulting in rough wrinkles on the surface of the skin and a marked decrease in elasticity.

At present, various studies have been conducted to investigate the mechanism of aging of the skin and various causes of skin aging have been identified. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, when the skin is exposed to harmful free radicals or free radicals, lipid peroxide is produced by oxidative action in the body and causes modification of various proteins constituting the skin.

On the other hand, collagen and elastic elastin proteins, which make up most of the dermis (about 70-80% of the total dry weight of the skin), are the major proteins produced by fibroblasts, and the skin's mechanical firmness, tissue binding strength and elasticity (Journal of the american academy of dermatology, 21: 610-613 (1989)). Collagen decreases with age and with increasing exposure to ultraviolet light. Collagen is classified according to form and structural features, the most well known of which are Type I, II, III and IV. Collagen types I and III constitute the constituents of the cytoplasm of the dermis, and collagen type IV is the major component of DEJ (Dermal Epidermal Junction). Collagen type II is located between the epidermal and dermal layers of the skin to regulate the permeation and transport of substances between the two layers, to control the differentiation of adjacent skin cells and to maintain the firmness of the skin. Collagen and elastin, the major structural components of the skin, are either denatured or reduced in biosynthesis by natural or external stimuli, thereby accelerating skin aging. In addition, the expression and activity of collagenase is also a major factor in accelerating skin aging.

Wrinkle formation and deterioration of skin elasticity are affected not only by the amount of collagen and elastin and the like that form the matrix structure in the dermal layer, but also by the organized state of the component or the degree of interaction between the matrix and skin cells.

Integrin, a type of membrane protein present in skin cells, also plays an important role in skin cell adhesion and cell-protein interactions, which in turn affects skin elasticity. It has been reported that the level of integrin expression is related to the degree of cell adhesion, that is, the skin elasticity. When the level of integrin expression on the surface of fibroblasts induced aging by UV-A and UV-B irradiation is measured It is reported that the level of integrin expression is lower than the level of integrin expression of unirradiated cells (Laetitia Moreau, Cosmetics & Toiletries, 118 (1): 75-84 2003).

Retinoic acid, epidermal growth factor (EGF), TGF (trans-forming growth factor), carcinogenesis factor Cardinale G. et al., Adv.Enzymol., 41, p 425, 1974), animal placental proteins (JP8-231370), betulinic acid (betulinic acid (JP8-208424)), chlorella extracts (JP9-40523, JP10-36283, fibroblast proliferation). . In particular, epidermal growth factor acts as a potent promoter of epithelial cell, endothelial cell and fibroblast cell proliferation, and promotes the movement and proliferation of epithelial cells during epithelial cell defects. Retinol is also described in US Pat. Nos. 4,603,146 and 4,877,805, which have a very effective effect on collagen synthesis and inhibition of degradation to exert skin wrinkle improvement.

However, retinoic acid is unstable, and there is a limit to the amount of use due to stability problems such as irritation and redness when applying the skin. Chlorella extract and the like have little effect, so the collagen synthesis of the skin cannot be substantially expected to improve skin function.

Therefore, there is an urgent need for the development of new collagen synthesis promoters which are safe for living bodies and are more effective than conventional collagen synthesis ability promoting substances.

The present invention is to solve the problems of the prior art as described above, can be applied stably without irritation to the skin, to promote the proliferation of skin cells, to inhibit the synthesis and synthesis of collagen synthesis of fibroblasts and to enhance the expression of integrin The purpose of the present invention is to provide a cosmetic composition capable of preventing skin aging by improving skin wrinkles and improving skin elasticity by having an excellent antioxidant effect.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing skin aging containing a roe deer urine extract as an active ingredient. The roe deer urine extract is effective in showing the effect of iii) promoting the proliferation of skin cells, ii) promoting the synthesis of collagen, iii) inhibiting collagenase, iii) enhancing integrin expression, iii) antioxidant activity, or iii) a combination thereof. It is contained as an ingredient. At this time, the roe deer urine extract is preferably contained in 0.001% to 20.0% by weight relative to the total weight of the composition.

According to the present invention, the roe deer urine extract is stable without skin irritation, and penetrates the skin to promote the proliferation of skin cells, promote the synthesis of collagen, inhibit collagenase, enhance the expression of integrin, and have antioxidant effects. Improves wrinkles and elasticity of the skin, effectively preventing skin aging and maintaining healthy skin.

Hereinafter, the present invention will be described in detail.

The present inventors conducted a study to search for skin aging agonist substances in native plants and animals, and as a result, the roe deer urine extract promotes the proliferation of skin cells, promotes collagen synthesis and inhibits degradation, enhances the expression of integrin, and antioxidant effects. It confirmed the outstanding thing and completed this invention.

Roe pee ( Astilbe) chinensis var. davidii ) is a perennial herb belonging to the genus Ophidae, commonly found in valleys all over the country, 30 ~ 70cm high, with long brown hairs, and rhizomes are thick and stretched shortly to the side. The leaves are divided into two or three times, three to three, and the small leaves are oval, 3 to 8 cm long, 2 to 4 cm wide, thin like paper, and have claws or crustal serrates on the edges and long petioles. Flowers bloom in July-August, reddish purple, with cones at the end of stems, about 30cm long, with many flowers running, flowers with brown hairs, calyxes split into 5, cracked pieces in the shape of eggs, 5 petals, linear It has 10 stamens, 2 pistils and 3 ~ 4mm long. These roe deer urine outposts are called horse riding horses Astilbes Herba and the roots of red horse riding horses Astilbe Radix are used for medicinal purposes. It is effective in releasing, lowering heat, releasing poison, relieving cramps, and relieving pain. It is used to solve (Korean medicinal plants, Kyohaksa, 2000, p204, exhaust ventilation base). In the private sector, it is used for fever, analgesia and anti-inflammatory, which is called red horse, and the part that grows on the ground called micro horse riding has antipyretic and antitussive action, so it is caused by cold, cough, It was prescribed and used for headaches and aches and pains. It has been reported that it contains hydrocyanic acid in the outpost, quercetin in the flowers, and vergenin and tannin in the rhizome. Plants belonging to the genus include A. divaricata, A. koreana and A. simplicifolia.

The cosmetic composition according to the present invention is included in the present invention as long as it contains such a roe deer urine extract. At this time, the roe deer urine extract is contained as an active ingredient to prevent skin aging. The roe deer urine extract is preferably extracted from the root of the roe deer urine extract using a solvent. The roe deer extract can be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol) Etc.), (c) acetone, (c) ethyl acetate, (e) chloroform, and (f) one, two or more mixed extraction solvents selected from the group consisting of 1,3-butylene glycol. have.

In the present invention, the roe deer urine extract is included in the present invention as long as it exhibits substantially the same effect using other extraction solvents as well as the aforementioned extraction solvents. In addition, the roe deer urine extract in the present invention includes not only an extract by the extraction solvent, but also an extract that has undergone a conventional purification process.

Specifically, the roe deer urine extract used in the present invention is used as it is, or extracted by the extraction solvent, after purification, for example, membrane separation membrane separation, such as an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography Purification procedures such as separation by (made for separation according to size, charge, hydrophobicity or affinity) can be used in addition. In addition, the roe deer urine extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying (or spray drying), and then contained in the composition as it is, or diluted in a solvent.

The roe deer urine extract, preferably contained in 0.001% to 20.0% by weight relative to the total weight of the cosmetic composition. At this time, when the roe deer urine extract is contained less than 0.001% by weight, the skin anti-aging effects such as promoting cell proliferation, promoting collagen synthesis, inhibiting collagen degradation, enhancing integrin expression, and antioxidant effects are insignificant, and are added in excess of 20.0% by weight. In this case, the synergy effect of excess input may be small and economically undesirable. More preferably, the roe deer urine extract is preferably contained in 0.01% by weight to 10.0% by weight relative to the total weight of the composition, even more preferably contained in 0.1% by weight to 5.0% by weight. More preferably, the cosmetic composition of the present invention comprises 0.01 to 10.0% by weight of the extract, even more preferably 0.1 to 5.0% by weight.

According to the present invention, the roe deer urine extract has an action of promoting the growth of skin cells, promoting the synthesis of collagen, inhibiting collagenase, enhancing the expression of integrin, excellent antioxidant action, etc. to improve skin wrinkles and elasticity of the skin Prevent skin aging. This fact is specified in the following examples.

The cosmetic composition according to the present invention includes components commonly used in cosmetic compositions in addition to the roe deer urine extract, and may include, for example, conventional auxiliaries such as stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

In addition, the cosmetic composition according to the present invention may be prepared in the form of a general formulation in the art, for example, an emulsion formulation or a solubilized formulation. Examples of the emulsified formulations include nutrient cosmetics, creams, essences, and the like. Examples of solubilized formulations include softening cosmetics. In addition, the cosmetic composition of the present invention can be prepared in the form of adjuvants that can be applied topically or systemically, which is commonly used in the field of dermatology by containing a dermatologically acceptable medium or base in addition to cosmetics.

Suitable formulations of cosmetics include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics, obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. Can be provided in the form of a vesicle dispersant, cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the roe extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic Or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other commonly used in cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological fields, such as ingredients. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc. can be mentioned.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows, patches, sprays and the like.

The cosmetic composition according to the present invention described above effectively prevents skin aging through the improvement of wrinkles and elasticity of the skin by the roe deer urine extract. Specifically, according to the present invention, the roe deer urine extract improves skin wrinkles by showing the action of promoting the proliferation of skin cells, promoting the synthesis of collagen, inhibiting the action of collagenase, enhancing the expression of integrin, excellent antioxidant action, etc. Prevents skin aging by promoting elasticity. This fact is specified in the following examples.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are provided to explain the present invention in more detail, and it will be apparent to those skilled in the art that the technical scope of the present invention is not limited by these examples. will be.

[ Production Example ]

<Preparation of roe deer urine extract>

Roots of roe deer urine were washed thoroughly and dried well, then broken into small pieces, precipitated in an aqueous solution containing ethyl alcohol and water at 7: 3, extracted for 5 days, filtered through a 300 mesh filter cloth, and filtered through a filter paper of Wattman 5 again. Then, it dried with the rotary vacuum evaporator and obtained dry weight 47g / kg. The dried roe deer urine extract powder was dissolved in 50% 1,3-butylene glycol and used as follows.

[ Experimental Example  One]

Skin cell proliferation effect experiment

In order to examine the effect of roe deer urine extract on skin cell proliferation, human normal fibroblasts (Korea Cell Line Bank) were inoculated to 1 x 10 ⁴ cells in each well of a 96-well microplate, and DMEM Incubated at 37 ° C. for 24 hours in medium (Sigma). Subsequently, the experimental group in which the roe deer urine extract of the preparation was replaced with the serum-free DMEM medium containing the final concentrations of 0.01%, 0.05%, 0.1%, 0.5%, and 1% and the DMEM medium without serum containing the roe deer extract Controls replaced with were further incubated for 24 hours. Then, 10 μl each of MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added thereto, followed by incubation for 4 hours. The medium was removed, shaken for 20 minutes by adding 100 μl of DMSO solution per well, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA). The measured values were substituted into the following Equation 1 to calculate the cell proliferation effect, and the results are shown in the following [Table 1].

Roe deer urine extract (%) Absorbance (570nm) Cell proliferation effect (%) Control 0.581 - 0.01 0.655 12.7 0.05 0.723 24.4 0.1 0.834 43.5 0.5 0.964 65.9 1.0 1.027 76.8

As shown in [Table 1], compared to the control group not treated with roe deer urine extract, it can be seen that the experimental group treated with roe deer urine extract showed a very high fibroblast proliferation effect.

[ Experimental Example  2]

<Collagen Synthesis Enhancement Experiment>

Human normal fibroblasts (Korea Cell Line Bank) were seeded to 2 x 10 ⁴ cells in each well of a 96-well microplate and incubated for 24 hours at 37 ° C in DMEM medium. Subsequently, the experimental group in which the roe deer urine extract of the above preparation was replaced with serum-free DMEM medium containing the final concentrations of 0.01, 0.05, and 0.10% and the control group replaced with the serum-free DMEM medium without the extract were further added for 48 hours. Incubated. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a kit (Takara, Japan). The measured amount was converted into ng / 2 × 10 ⁴ cells, and the results are shown in the following [Table 2].

Roe deer urine extract (%) Collagen synthesis amount (ng / 2 x 10 ⁴ ) Control 102 0.01 119 0.05 148 0.10 192

As shown in [Table 2], compared to the control group not treated with the roe deer urine extract, it can be seen that the experimental group treated with roe deer urine extract showed a very high collagen synthesis amount. And as the concentration of roe deer urine extract increased, it showed that the roe deer urine extract showed an excellent collagen synthesis enhancing effect.

[ Experimental Example  3]

< Collagenase  Inhibitory Effect Experiment>

To determine the collagenase production inhibitory ability of roe deer urine extract was measured as follows.

Experiments were performed by placing human fibroblasts into 2x10 ⁴ cells / well in a 96-well microtiter plate containing Dulbecco's Modified Eagle's Media (DMEM) medium containing 2.5% fetal calf serum. Incubate until 70-80% growth. And after treating the roe deer urinary extract of the preparation for 24 hours for each concentration, the cell culture was collected. The cultured cell culture solution was measured by using a commercially available collagenase measuring instrument (Amersham Pharmacia Co., Catalog #: RPN 2610). First, the cultured cells were placed in a 96-well plate uniformly coated with primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours. After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, the coloring stimulant was added to cause color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the reaction (color development). The color of the reaction solution was yellow and the degree of yellow color was different according to the progress of the reaction. The absorbance of the yellowish 96-well plate was measured at 405 nm using an absorbance meter and the degree of synthesis of collagenase was calculated. At this time, the reaction absorbance of the prepared cell culture medium of the group not treated with the composition was used as a control. As such, as a result of measuring the inhibition of collagenase expression in cells, it was confirmed that the experimental group inhibited collagenase expression as shown in Table 3 below in vitro. Expression inhibition capacity is prepared by setting the synthetic ability of the untreated group (control) to 100.

Roe deer urine extract (%) Collagenase expression level (%) Control 100 0.01 85 0.05 75 0.10 69

As shown in [Table 3], as a result of measuring collagenase expression inhibition in cells, it was confirmed that the roe deer urine extract inhibited the expression of collagenase.

[ Experimental Example  4]

< Integreen  β1 Expression Enhancement Effect Experiment>

Human normal fibroblasts were seeded to 1 × 10 6 cells in each well of a 48-well microplate and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the experimental group in which the roe deer urine extract of the preparation was replaced with the serum-free DMEM medium containing the final concentrations of 0.01%, 0.05%, 0.1%, 0.5%, and 1% and the DMEM medium without serum containing the roe deer extract Controls replaced with were further incubated for 24 hours. After incubation, the expression level of integrin β1 was measured by EIA (Enzyme immnoassay) method (MK008 kit, Takara, Japan). Specifically, the cells were recovered by centrifugation, the cell extracts were prepared, and the cell extracts were treated in wells coated with anti-integrin β1 antibody. Secondary antibody was added and reacted at 4 ° C. for 45 minutes and then detected by OPD (ο-Phenylenediamine-HCl). The stained OPD was dissolved in the OPD solution, and then absorbance was measured by using an ELISA reader at 530 nm. The results are shown in the following [Table 4].

Roe deer urine extract (%) Absorbance (570nm) 0.01 0.592 0.05 0.614 0.10 0.650 0.50 0.736 1.00 0.841

As shown in [Table 4], it was confirmed that the roe deer urine extract promotes the synthesis of integrin β1 which plays an important role in cell adhesion in a concentration-dependent manner.

[ Experimental Example  5]

<Antioxidant Effect Test>

To investigate the antioxidant effect of roe deer urine extract, Free Radical Scavenging Activity Test was conducted as follows.

The free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), and the stable free radical DPPH (1,1-diphenyl-2- picryhydrazyl (Sigma Co., Ltd.) reagent was used. 150 μl of DPPH solution (ethanol in the case of Blanc) was prepared at various concentrations, and 150 μl of these extracts were mixed, mixed, and allowed to stand at room temperature for 30 minutes, followed by 517 nm. An experimental group for measuring absorbance was set, and the control group was set to the case using purified water. After absorbance measurement for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below and the results are shown in the following [Table 5].

Roe deer urine extract (%) Free radical scavenging effect (%) 0.01 18.5 0.05 30.1 0.10 48.6 0.50 69.8 1.00 86.2

As shown in [Table 5], the roe deer urine extract was confirmed that the free radical scavenging ability is very excellent.

[ Example ]

In order to conduct a clinical test on the skin wrinkle and elasticity improving effect of the cosmetic containing the roe deer urine extract, a cosmetic composition was prepared with the ingredients and contents as shown in the following [Table 6]. Specific manufacturing method is as follows.

1) The components 1-6 shown in the following [Table 6] were heated to 70 ° C. while mixing and stirring to prepare an oily solution.

2) 7-8 and 10-11 were added to purified water and warmed to 70 ° C. while stirring to prepare an aqueous solution.

3) After mixing 1) and the raw material 9 which are oily solutions to the aqueous solution of said 2), it emulsified and cooled.

4) The raw materials 13 and 14 were added to 3), mixed, and cooled to 30 ° C.

[ Comparative example ]

Except for containing the roe deer urine extract was prepared in the same manner as in the above example. Specific components and contents of this Comparative Example are as shown in the following [Table 6].

ingredient Content (% by weight) Example Comparative example  1. Vaseline 7.0 7.0  2. Liquid paraffin 5.0 5.0  3. Beeswax 2.0 2.0  4. Polysorbate-60 2.0 2.0  5. Solbitan Sesquioleate 2.5 2.5  6. Squalane 3.0 3.0  7. Propylene Glycol 6.0 6.0  8. Glycerin 4.0 4.0  9. Triethanolamine 0.5 0.5  10. Carboxyvinyl Polymer 0.5 0.5  11.Tocopherol Acetate 0.1 0.1  12. Purified Water To 100 To 100  13. roe deer urine extract 2.0 2.0  14. Incense, preservative a very small amount a very small amount

[ Experimental Example  6]

<Skin wrinkle improvement effect experiment>

To determine the skin wrinkle improvement effect of the cosmetic composition containing a roe deer urine extract according to the present invention was measured in 20 healthy women aged 35 to 50 years.

First, A and B were divided into two groups, and the group A was given an example, and the group B was applied to the formulation of the comparative example twice a day (morning and evening) for 3 months. After 3 months, the degree of wrinkle improvement was evaluated by subject's questionnaire and image analysis of wrinkles.

The subject's questionnaire was judged as four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to before use, and the results are shown in the following [Table 7].

In addition, for image analysis of wrinkles, a replica under the eye is taken before the experiment is started, and a replica immediately after the experiment is completed at the same site under the eye. The density was measured. The measurement results of wrinkle density by image analysis are shown in Table 8 below, calculated as a reduction ratio against wrinkle density before use.

sample No improvement Minor improvements Moderate improvement A significant improvement Example One One 4 4 Comparative example 7 - -

sample Wrinkle Density Reduction (%) Example 45 Comparative example 10

As shown in [Table 7] and [Table 8], in the case of the Example containing roe deer urine extract, it was found that the skin wrinkle improvement effect is very excellent compared to the comparative example.

[ Experimental Example  7]

<Skin Elasticity Test>

According to the present invention to determine the skin elasticity enhancing effect of the cosmetic composition containing the roe deer urine extract was measured as follows.

20 healthy women (average age 37 years) of 20 years or older (average age 37 years) were divided into two groups A and B, and the formulation of Comparative Example was compared to group B. After applying twice a day (morning and evening) for 12 weeks, the skin elasticity was measured using a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, Germany). The results are shown in the following [Table 9]. The results were expressed as R8 (R8 (12 weeks) -R8 (0 weeks)) of cutometer MPA580, and R8 value is a property of skin viscoelasticity.

sample Skin elasticity effect Example 0.67 Comparative example 0.29

As shown in Table 9, the Example containing the roe deer urine extract was confirmed that the skin elasticity effect is very excellent compared to the comparative example.

[ Experimental Example  8]

Formulation Stability Experiment

According to the present invention was carried out in the following method to determine the formulation stability for the cosmetic containing the roe deer urine extract.

First, in order to test the stability of the cosmetics, the cosmetic composition according to the above Examples and Comparative Examples was kept in an opaque glass container in a thermostat maintained at 45 ℃ constant for 12 weeks, and also completely shaded at 4 ℃ After storing for 12 weeks in an opaque glass container in the refrigerator, discoloration, deodorization, and separation were measured. The results are shown in Table 10 below. At this time, the product discoloration, odor, and separation were evaluated by classifying into the following six grades.

<Grade>

0: no change

1: very little separation (discoloration)

2: A little separation (discoloration)

3: Slightly separated (discolored)

4: severely separated (discolored)

5: extremely severe separation (discoloration)

 division Formulation Stability Example Comparative example 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0.5 0 0 0 Deodorant 0.3 0 0 0 detach 0 0 0 0

As shown in Table 10, the cosmetic compositions in Examples and Comparative Examples were stable at 45 ° C. with little discoloration, discoloration, or separation, indicating that the roe deer urine extract was present in a stable state in the formulation.

Hereinafter, on the basis of the results of the above experimental example will be presented a formulation example of the cosmetic composition containing a roe deer urinary extract according to the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

[ Formulation example  One]

<Softener (Skin Lotion)>

According to the conventional method with the components and contents shown in the following [Table 11] was prepared a flexible cosmetic water.

ingredient Content (% by weight) Roe deer urine extract 0.5 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water To 100

[ Formulation example  2]

<Nutritional Cosmetics ( Milk Croissant )>

To nutrient cosmetics was prepared according to the conventional method with the ingredients and contents shown in the following [Table 12].

ingredient Content (% by weight) Roe deer urine extract 1.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 BH 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water To 100

[ Formulation example  3]

<Nutrition Cream>

Nutritional creams were prepared according to a conventional method with the ingredients and contents shown in the following [Table 13].

ingredient Content (% by weight) Roe deer urine extract 2.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctan Inn 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Liquid paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water To 100

[ Formulation example  4]

<Massage cream>

Massage creams were prepared according to a conventional method with the ingredients and contents shown in the following [Table 14].

ingredient Content (% by weight) Roe deer urine extract 0.5 Propylene glycol 6.0 Glycerin0 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxyvinyl Polymer 0.5 Incense, preservative a very small amount Purified water To 100

[ Formulation example  5]

<Pack>

A pack was prepared according to a conventional method with the ingredients and contents shown in the following [Table 15].

ingredient Content (% by weight) Roe deer urine extract 1.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 BH 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water To 100

As described above, the cosmetic composition according to the present invention contains the roe deer urine extract has an effect of preventing skin aging. Specifically, roe deer urine extract promotes the proliferation of skin cells, inhibits the synthesis and synthesis of fibroblasts collagen, promotes the expression of integrins, has an excellent antioxidant effect, improves skin wrinkles and improves the elasticity of the skin, thereby improving skin aging. prevent. In addition, the cosmetic composition of the present invention is a safe substance having no skin irritation, and may be usefully used in the formulation of lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

Claims (5)

A cosmetic composition for preventing skin aging, comprising a roe deer urine extract. The method of claim 1, The roe deer urine extract is a cosmetic composition for preventing skin aging, characterized in that contained in 0.001 ~ 20.0% by weight based on the total weight of the composition. The method of claim 1, Skin aging, characterized in that the extract is extracted by one or more mixed solvents selected from water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform and 1,3-butylene glycol Preventive cosmetic composition. The method of claim 1, The roe deer urine extract exhibits the action of i) promoting the proliferation of skin cells, i) promoting the synthesis of collagen, i) inhibiting collagenase, i) enhancing the expression of integrin, i) antioxidant activity, or i) a combination thereof. Cosmetic composition for preventing skin aging, characterized in that. The method of claim 1, The cosmetic composition is a cosmetic composition for preventing skin aging, characterized in that it has a formulation of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.