KR102741855B1 - Antioxidant cosmetic compositions comprising plant extracts having antioxidant activity - Google Patents

Abstract

The present invention provides an antioxidant cosmetic composition comprising a plant extract having antioxidant activity, and specifically, provides a cosmetic composition having an excellent antioxidant effect by including at least one selected from among a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract as an active ingredient.

Description

{Antioxidant cosmetic compositions comprising plant extracts having antioxidant activity}

The present invention relates to an antioxidant cosmetic composition comprising a plant extract having antioxidant activity. More specifically, the present invention relates to a cosmetic composition having an excellent antioxidant effect by including at least one selected from among a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract as an active ingredient.

The skin is a membrane that covers the outside of the body and performs various physiological functions to protect the body from various external stimuli, obstacles, dryness, and other environmental factors, and plays a role in protecting and regulating internal organs and other body systems.

However, active oxygen that enters from outside the body or is generated inside the body can accelerate the aging of the body or cause cancer. Active oxygen reacts with the SH group of proteins to cause enzymes to lose their activity or form cross-links, and oxidizes unsaturated fatty acids that make up DNA, RNA, enzymes, and cell membranes to form lipid peroxides. Lipid peroxides are very toxic to the body and lower the body's functions, eventually causing diseases such as arteriosclerosis, blood circulation disorders, fatigue, diabetes, heart disease, liver disorders, carcinogenesis, and cancer metastasis, and ultimately becoming the cause of aging.

Previously, research on antioxidants that suppress oxidation by active oxygen was conducted, but there is a problem that antioxidants that exist naturally cannot be expected to have sufficient effects when applied to the skin, and the use of synthetic antioxidants with excellent oxidizing power and low price is limited due to safety concerns such as side effects on the human body.

To overcome these limitations, prior patents related to cosmetic compositions with antioxidant activity using plant extracts include Korean Patent Application No. 20020047048 entitled “Cosmetic composition comprising a thorny tree extract having antioxidant activity and a method for producing the extract,” Korean Patent Application No. 20080107420 entitled “Cosmetic composition for antioxidant and skin aging prevention containing a burdock extract,” and Korean Patent Application No. 20050021778 entitled “Cosmetic composition having an antioxidant effect containing a lotus extract as a main ingredient.”

In this way, there is a wide demand for the development of a useful cosmetic composition having ABTS radical scavenging ability and antioxidant efficacy that inhibits cell oxidation, and there is also a demand for the development of a cosmetic composition that has almost no side effects and can be used for a long period of time without any problems, and in addition, there is a demand for various plant materials.

1. Republic of Korea Patent Application No. 20020047048 2. Republic of Korea Patent Application No. 20080107420 3. Republic of Korea Patent Application No. 20050021778

The present invention aims to provide a useful cosmetic composition having ABTS radical scavenging ability and antioxidant efficacy that inhibits cell oxidation while utilizing a natural plant extract.

Accordingly, the purpose of the present invention is to provide an antioxidant cosmetic composition containing at least one selected from among a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract as an effective ingredient.

In order to solve the above-described problems, the present invention provides an antioxidant cosmetic composition comprising at least one selected from among a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract as an effective ingredient.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that it contains two or more selected from a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract as effective ingredients.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that the Juglans mandshurica extract is an extract of at least one part selected from the group consisting of a stem, a leaf, a fruit, a flower, and a root, the Euyale ferox extract is an extract of at least one part selected from the group consisting of a whole plant, a leaf, a fruit, a flower, and a root, the Rhodiola rosea extract is an extract of at least one part selected from the group consisting of a whole plant, a stem, a leaf, a fruit, a flower, and a root, and the Epilobium angustifolium extract is an extract of at least one part selected from the group consisting of a whole plant, a stem, a leaf, a fruit, a flower, and a root.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that the extract is an extract extracted using at least one solvent selected from the group consisting of water, methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, and glycerin.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that the extract is an extract extracted by at least one method selected from a reflux extraction method, a hot water extraction method, a hot water extraction method, an immersion extraction method, and an ultrasonic extraction method.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that the extract is extracted by hot water extraction at 50-130°C for 5 minutes to 10 hours or by immersing in an organic solvent at 5-40°C for 6 hours to 5 days.

According to one embodiment of the present invention, the extract provides an antioxidant cosmetic composition characterized by having ABTS radical scavenging ability.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that each of the extracts is independently included in an amount of 0.001 to 25 wt% relative to the total weight of the cosmetic composition.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that when two or more extracts from the Juglans mandshurica extract, the Euyale ferox extract, the Rhodiola rosea extract, and the Epilobium angustifolium extract are included, the respective extracts are included in a weight ratio of 0.5 to 1.5: 0.5 to 2: 0.1 to 2: 0.5 to 2.

According to one embodiment of the present invention, an antioxidant cosmetic composition is provided, characterized in that the cosmetic composition has at least one formulation selected from a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray.

According to the present invention as described above, a cosmetic composition is provided which contains at least one selected from among an extract of Juglans mandshurica , an extract of Euyale ferox , an extract of Rhodiola rosea , and an extract of Epilobium angustifolium as an effective ingredient.

Through this, the present invention can provide a useful cosmetic composition having an antioxidant effect that suppresses cell oxidation while having ABTS radical scavenging ability while using a natural plant extract.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

The advantages and features of the present invention, and the method for achieving them, will become clear with reference to the embodiments described below. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and these embodiments are provided only to make the disclosure of the present invention complete and to fully inform a person skilled in the art of the scope of the present invention, and the present invention is defined only by the scope of the claims.

The terminology used herein is for the purpose of describing embodiments only and is not intended to be limiting of the invention. In this specification, the singular also includes the plural unless the context clearly dictates otherwise. The terms "comprises" and/or "comprising" as used herein do not exclude the presence or addition of one or more other elements other than the mentioned elements. The same reference numerals throughout the specification refer to the same elements, and "and/or" includes each and every combination of one or more of the mentioned elements.

Unless otherwise defined, all terms (including technical and scientific terms) used in this specification may be used with the meaning commonly understood by those skilled in the art to which the present invention belongs. In addition, terms defined in commonly used dictionaries shall not be ideally or excessively interpreted unless explicitly specifically defined.

The present invention provides an antioxidant cosmetic composition comprising, as an effective ingredient, at least one selected from a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract.

The term "juglans mandshurica " used in the present invention is a deciduous broad-leaved tree of the juglans family in the juglans order, a tree that grows abundantly in Korea (central and north), northeastern China, and Siberia (Amur and Ussuri). It is also called jujamok (楸子木) and its fruit is called juja (楸子). It grows on the sunny side of the mountainside and is about 20 m tall. The bark is dark gray and splits vertically. The leaves are odd-pinnate. There are 7 to 17 leaflets, which are oblong or egg-shaped oval, 7 to 28 cm long, and about 10 cm wide. It has fine teeth, the front side has fine hairs that gradually disappear, and the back side may or may not have hairs, and there are glandular hairs on the leaf veins. The flowers are unisexual, blooming in April. The male spike is 10–20 cm long, has 12–14 stamens, and 4–10 flowers bloom on the female spike. The fruit is a drupe, ovate and spherical, 4–8 cm long, ripening in September. The outer skin is densely covered with glandular hairs, and the endocarp is dark brown, very hard, and pointed at both ends.

The term "thorny lotus" ( Euyale ferox ) used in the present invention is a southern annual aquatic plant of the Nymphalidae family, which lives in old ponds, reservoirs, and lakes in the central and southern regions. It has the largest leaves among the native plants in Korea, reaching a diameter of 2 m. Most of the plant, such as the leaves, stems, and fruits, are covered with thorns, and the fruits turn black when ripe, and the seeds are dried and used as medicine or food.

The term " Rhodiola rosea " used in the present invention refers to a perennial herb of the Rhodiolaceae family of the rose order, a dicotyledonous plant that grows in crevices of rocks in mountainous regions. It is about 7 to 30 cm tall, white all over, grows in clumps, and the lower part is covered with brown scales. The leaves are alternate, fleshy, egg-shaped or oval, and have dull serrations on the upper margin. The flowers are unisexual, blooming in July to August, light yellow but sometimes with a purple tinge, and form cymes. The male flowers have a degenerate pistil and 8 to 10 stamens, and the female flowers have 4 to 5 pistils. The fruits are 4 to 5 pods, 6 to 7 mm long, and ripen in September.

As used herein, the term " Epilobium angustifolium ," also known as rose bay or willow herb, is a species of the genus Chamerion in the family Apiaceae, found in the Northern Hemisphere of the Earth. This plant is known to be used particularly in the production of mountain honey. Its leaves, when used in herbal tea, have anti-migraine action and action against sleep disorders. They also have healing properties.

The term "extract" used in the present invention includes all formulations that can be formed using the extract, such as the extract obtained by extract treatment of a plant, a diluted or concentrated extract, a dried product obtained by drying the extract, a conditioned or purified product of the extract, or a mixture thereof. The extract of the present invention can be preferably manufactured and used in the form of a dried powder after extraction.

According to one embodiment of the present invention, two or more selected from a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract may be included as active ingredients. When a combination of two or more is included in this way, a synergistic effect in terms of antioxidant activity can be obtained. The combination of two or more includes a combination of two, three, or all four arbitrarily selected from the four extracts. Specifically, the cosmetic composition of the present invention may include all of a Juglans mandshurica extract, a Euyale ferox extract, a Rhodiola rosea extract, and an Epilobium angustifolium extract.

According to one embodiment of the present invention, the Juglans mandshurica extract may be an extract of at least one part selected from the group consisting of a stem, a leaf, a fruit, a flower, and a root, the Euyale ferox extract may be an extract of at least one part selected from the group consisting of a whole plant, a leaf, a fruit, a flower, and a root, the Rhodiola rosea extract may be an extract of at least one part selected from the group consisting of a whole plant, a stem, a leaf, a fruit, a flower, and a root, and the Epilobium angustifolium extract may be an extract of at least one part selected from the group consisting of a whole plant, a stem, a leaf, a fruit, a flower, and a root.

According to one embodiment of the present invention, the Juglans mandshurica extract may be an extract of the fruit, leaf, and stem, the Euyale ferox extract may be an extract of the whole plant, the Rhodiola rosea extract may be an extract of the whole plant, and the Epilobium angustifolium extract may be an extract of the whole plant.

According to one embodiment of the present invention, the extract may be an extract extracted using at least one solvent selected from the group consisting of water, methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, and glycerin. Specifically, the extract may be an extract extracted using water or ethanol, or a mixed solvent of water and ethanol.

According to one embodiment of the present invention, the extract may be an extract extracted by at least one method selected from a reflux extraction method, a hot water extraction method, a hot water extraction method, an immersion extraction method, and an ultrasonic extraction method.

According to one embodiment of the present invention, the extract can be extracted by hot water extraction at 50-130°C for 5 minutes to 10 hours, or by soaking in an organic solvent at 5-40°C for 6 hours to 5 days. Although not limited thereto, in the case of extraction using water, it is preferable to perform hot water extraction at 50-130°C for 5 minutes to 10 hours, and in the case of extraction using an organic solvent such as ethanol, it is preferable to perform extraction by soaking in an organic solvent at 5-40°C for 6 hours to 5 days. Specifically, in the case of extraction using water, it can be performed by hot water extraction at 120-130°C for 10 minutes to 30 minutes, and in the case of extraction using an organic solvent such as ethanol, it can be performed by soaking at 5-40°C for 0.5 day to 2 days.

The weight of the above extraction solvent is not particularly limited, but if an insufficient amount is used, the extract cannot be sufficiently extracted, and if an excessive amount is used, it takes a lot of time and money to remove the extraction solvent and obtain a dry extract. If hot water extraction is performed, it is necessary to sufficiently include the sample and perform the extraction in a heated state to increase the extraction efficiency. At this time, it should be noted that if the temperature is too low, it is difficult to fully utilize the benefits of heating and a long extraction time is required, and if the temperature is too high, decomposition of useful substances may occur. In addition, in the case of organic solvent extraction, extraction can be performed at room temperature, and the sample can be immersed in a sufficient extraction solvent for about 0.5 days or more to ensure sufficient extraction. If the extraction is insufficient, the extraction amount can be increased through a repeated process or by increasing the extraction time.

According to one embodiment of the present invention, the extract may have ABTS radical scavenging activity. Specifically, the extract of the present invention may have an ABTS radical scavenging activity (activity) of 1 to 60% calculated by the following formula at a concentration of 0.1 to 100 μg/㎖, individually or in combination of two or more. More specifically, the extract of the present invention may have an ABTS radical scavenging activity (activity) of 1 to 60% at a concentration of 0.1 to 20 μg/㎖, individually or in combination of two or more.

[Antioxidant activity calculation formula]

According to one embodiment of the present invention, the extracts may be independently included in an amount of 0.001 to 25 wt% based on the total weight of the cosmetic composition. Specifically, they may be included in an amount of 0.01 to 20 wt%, and more specifically, they may be included in an amount of 0.1 to 10 wt%. When included in the above range, the antioxidant activity may be significantly exhibited.

According to one embodiment of the present invention, when two or more extracts from the Juglans mandshurica extract, the Euyale ferox extract, the Rhodiola rosea extract, and the Epilobium angustifolium extract are included, the respective extracts may be included in a weight ratio of 0.5 to 1.5 : 0.5 to 2 : 0.1 to 2 : 0.5 to 2. Specifically, it may be 0.7 to 1.2 : 0.7 to 1.5 : 0.3 to 1.5 : 0.7 to 1.5. When included within the above range, the respective extracts may be mixed and useful for exhibiting excellent antioxidant activity.

According to one embodiment of the present invention, the cosmetic composition may have at least one formulation selected from a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray.

According to one embodiment of the present invention, the cosmetic composition may further contain adjuvants commonly used in the cosmetic field, such as fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics.

Hereinafter, embodiments of the present invention will be described in detail.

1. Preparation of extract of the present invention

A mixture of the fruit, leaves, and stems of Juglans mandshurica , and the dried materials of the whole plant of Euyale ferox, Rhodiola rosea , and Epilobium angustifolium were uniformly ground, 10 g of which was soaked in 100 mL of ethanol for 18 to 72 hours, filtered, and the ethanol extract obtained was concentrated using a rotary vacuum evaporator to obtain an extract.

2. Confirmation of the antioxidant capacity of the present invention

The concentrated extract obtained above was dissolved in DMSO at 25 mg/mL and used in an antioxidant activity experiment.

The extract dissolved in DMSO was treated at a final concentration of 1000 ug/mL to 1 ug/mL as described in the table below.

The ABTS reaction solution was prepared by mixing 7 mM 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2.4 mM potassium persulfate solution in a 1:1 ratio, reacting for 12–16 hours at room temperature in a darkened state, and then diluting with distilled water to an O.D. value of 1.5±0.03 at 414 nm. After that, 50 μl of the diluted extract and 150 μl of the ABTS reaction solution were added to a 96-well plate, reacted for 10 minutes at room temperature in a darkened state, and then measured for absorbance at 734 nm using a microplate instrument.

1) Comparison of each extract alone and in combination with two extracts

The results of evaluating and comparing the antioxidant activity of each of the extracts of the common maple, the extract of the prickly lotus, the extract of the rock stone flower, and the extract of the pink needle, alone and in combination, are as follows.

Phellodendron
(ug/mL) Thorn lotus
(ug/mL) Antioxidant capacity (%)
(A)

Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 1 5.26 - 25.20 48.17 Combination extract (Example 3) Antioxidant activity increased by 3.8% Example 2 - 5.26 22.97 Example 3 5.26 5.26 50.00 - Example 4 2.63 - 12.30 22.44 Combination extract (Example 6) Antioxidant activity increased by 3.8% Example 5 - 2.63 10.14 Example 6 2.63 2.63 26.81 - Example 7 3.52 - 16.70 48.41 Combination extract (Example 9) Antioxidant activity increased by 3.18% Example 8 - 7.05 31.71 Example 9 3.52 7.05 50.00 - Example 10 1.76 - 8.05 22.57 Combination extract (Example 12) Antioxidant activity increased by 12.68% Example 11 - 3.52 14.52 Example 12 1.76 3.52 25.84 -

Phellodendron
(ug/mL) Rockflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 13 2.63 - 12.31 48.54 The antioxidant capacity of the combined extract (Example 15) increased by 2.92% Example 14 - 2.63 36.23 Example 15 2.63 2.63 50.00 - Example 16 1.32 - 19.82 48.99 Combination extract (Example 18) antioxidant activity increased by 23.05% Example 17 - 1.32 29.17 Example 18 2.63 2.63 32.75 - Example 19 4.16 - 16.70 48.41 The antioxidant capacity of the combined extract (Example 21) increased by 3.18% Example 20 - 2.08 31.71 Example 21 4.16 2.08 50.00 - Example 22 2.08 - 9.61 25.42 The antioxidant capacity of the combined extract (Example 24) increased by 11.67% Example 23 - 1.04 15.81 Example 24 2.08 1.04 28.78 -

Phellodendron
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 25 4.74 - 22.65 35.25 Combination extract (Example 27) Antioxidant activity increased by 29.5% Example 26 - 4.74 12.60 Example 27 4.74 4.74 50.00 - Example 28 2.37 - 11.02 17.26 The antioxidant capacity of the combined extract (Example 30) increased by 35.27% Example 29 - 2.37 6.23 Example 30 2.37 2.37 26.66 - Example 31 3.20 - 15.11 32.20 The antioxidant capacity of the combined extract (Example 33) increased by 35.61% Example 32 - 6.40 17.08 Example 33 3.20 6.40 50.00 - Example 34 1.60 - 7.26 15.73 The antioxidant capacity of the combined extract (Example 36) increased by 41.80% Example 35 - 3.20 8.47 Example 36 1.60 3.20 27.02 -

Thorn lotus
(ug/mL) Rockflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 37 2.56 - 9.79 45.06 The antioxidant capacity of the combined extract (Example 39) increased by 9.89% Example 38 - 2.56 35.27 Example 39 2.555 2.56 50.00 - Example 40 1.28 - 3.56 22.42 The antioxidant capacity of the combined extract (Example 42) increased by 16.93% Example 41 - 1.28 18.86 Example 42 1.28 1.28 26.99 - Example 43 6.09 - 27.04 49.04 The antioxidant capacity of the combined extract (Example 45) increased by 1.92% Example 44 - 1.52 22.00 Example 45 6.09 1.52 50.00 - Example 46 3.04 - 12.18 24.41 The antioxidant capacity of the combined extract (Example 48) increased by 15.70% Example 47 - 0.76 12.23 Example 48 3.04 0.76 28.95 -

Thorn lotus
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 49 5.21 - 22.75 36.63 The antioxidant capacity of the combined extract (Example 51) increased by 26.74% Example 50 - 5.21 13.88 Example 51 5.21 5.21 50.00 - Example 52 2.61 - 10.04 16.91 The antioxidant capacity of the combined extract (Example 54) increased by 38.83% Example 53 - 2.61 6.87 Example 54 2.61 2.61 27.64 -

Rockflower
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 55 2.53 - 34.95 41.61 The antioxidant capacity of the combined extract (Example 57) increased by 16.77% Example 56 - 2.53 6.67 Example 57 2.53 2.53 50.00 - Example 58 1.27 - 18.70 21.97 The antioxidant capacity of the combined extract (Example 60) increased by 22.49% Example 59 - 1.27 3.26 Example 60 1.27 1.27 28.34 - Example 61 1.52 - 22.03 38.29 The antioxidant capacity of the combined extract (Example 63) increased by 23.41% Example 62 - 6.10 16.27 Example 63 1.52 6.10 50.00 - Example 64 0.76 - 12.24 20.31 The antioxidant capacity of the combined extract (Example 66) increased by 28.54% Example 65 - 3.05 8.06 Example 66 0.76 3.05 28.42 -

As a result of the experiment, all examples were able to confirm the antioxidant activity. In addition, even though the same concentration was treated, all examples including the combined extract (Examples 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 25, 28, 51, 54, 57, 60, 63, 66) were able to confirm the results showing a higher antioxidant activity than the simple sum (B) of the antioxidant activity of each individual extract constituting them. Among these, in the case of Example 36 including the deciduous tree extract and the pink needle flower extract, the antioxidant value of the deciduous tree extract alone (Example 34) and the pink needle flower extract alone (Example 35) at the same concentration increased by a whopping 41.80%, showing a remarkably excellent effect.

3) Comparison of each extract alone and with three combinations

The results of evaluating and comparing the antioxidant activity of each of the extracts of the common maple, the extract of the prickly lotus, the extract of the rock stone flower, and the extract of the pink needle, alone and in combination, are as follows.

Phellodendron
(ug/mL) Thorn lotus
(ug/mL) Rockflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 67 2.05 - - 9.46 45.57 The antioxidant capacity of the combined extract (Example 70) increased by 8.86% Example 68 - 2.05 - 7.33 Example 69 - - 2.05 28.78 Example 70 2.05 2.05 2.05 50 - Example 71 1.03 - - 4.43 22.38 The antioxidant capacity of the combined extract (Example 74) increased by 27.02% Example 72 - 1.03 - 2.32 Example 73 - - 1.03 15.62 Example 74 1.03 1.03 1.03 30.66 - Example 75 2.12 - - 9.79 43.83 The antioxidant capacity of the combined extract (Example 78) increased by 12.33% Example 76 - 4.23 - 17.99 Example 77 - - 1.06 16.05 Example 78 2.12 4.23 1.06 50.00 - Example 79 1.06 - - 4.60 21.51 The antioxidant capacity of the combined extract (Example 82) increased by 30.53% Example 80 - 2.12 - 7.65 Example 81 - - 0.53 9.26 Example 82 1.06 2.12 0.53 30.96 -

Phellodendron
(ug/mL) Thorn lotus
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 83 3.18 - - 15.01 36.27 The antioxidant capacity of the combined extract (Example 86) increased by 8.86% Example 84 - 3.18 - 12.84 Example 85 - - 3.18 8.42 Example 86 3.18 3.18 3.18 50 - Example 87 1.59 - - 7.21 16.43 The antioxidant capacity of the combined extract (Example 90) increased by 44.10% Example 88 - 1.59 - 5.08 Example 89 - - 1.59 4.14 Example 90 1.59 1.59 1.59 29.39 - Example 91 1.93 - - 8.87 35.24 The antioxidant capacity of the combined extract (Example 94) increased by 29.51% Example 92 - 3.86 - 16.14 Example 93 - - 3.86 10.24 Example 94 1.93 3.86 3.86 50.00 - Example 95 0.96 - - 4.13 15.91 The antioxidant capacity of the combined extract (Example 98) increased by 45.71% Example 96 - 1.93 - 6.73 Example 97 - - 1.93 5.05 Example 98 0.96 1.93 1.93 29.31 -

Phellodendron
(ug/mL) Rockflower
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 99 1.98 - - 9.1 42.12 The antioxidant capacity of the combined extract (Example 101) increased by 15.76% Example 100 - 1.98 - 27.84 Example 101 - - 1.98 5.18 Example 102 1.98 1.98 1.98 50.00 - Example 103 0.99 - - 4.25 21.92 The antioxidant capacity of the combined extract (Example 106) increased by 30.85% Example 104 - 0.99 - 15.15 Example 105 - - 0.99 2.52 Example 106 0.99 0.99 0.99 31.70 - Example 107 2.34 - - 10.90 40.87 The antioxidant capacity of the combined extract (Example 110) increased by 29.51% Example 108 - 1.17 - 17.50 Example 109 - - 4.69 12.47 Example 110 2.34 1.17 4.69 50.00 - Example 111 1.17 - - 5.15 21.30 The antioxidant capacity of the combined extract (Example 114) increased by 19.71% Example 112 - 0.59 - 9.98 Example 113 - - 2.34 6.17 Example 114 1.17 0.59 2.34 26.53 -

Thorn lotus
(ug/mL) Rockflower
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 115 2.14 - - 7.78 43.34 The antioxidant capacity of the combined extract (Example 118) increased by 13.32% Example 116 - 2.14 - 29.94 Example 117 - - 2.14 5.62 Example 118 2.14 2.14 2.14 50.00 - Example 119 1.07 - - 2.55 21.49 The antioxidant capacity of the combined extract (Example 122) increased by 25.33% Example 120 - 1.07 - 16.20 Example 121 - - 1.07 2.74 Example 122 1.07 1.07 1.07 28.78 - Example 123 3.86 - - 16.15 41.24 The antioxidant capacity of the combined extract (Example 126) increased by 17.53% Example 124 - 0.96 - 14.84 Example 125 - - 3.86 10.24 Example 126 3.86 0.96 3.86 50.00 - Example 127 1.93 - - 6.73 20.44 The antioxidant capacity of the combined extract (Example 130) increased by 22.72% Example 128 - 0.48 - 8.65 Example 129 - - 1.93 5.05 Example 130 1.93 0.48 1.93 26.45 -

As a result of the experiment, it was confirmed that all examples had antioxidant activity. In addition, even though the same concentration was used, it was confirmed that all examples including the combined extract (Examples 70, 74, 78, 82, 86, 90, 94, 98, 102, 106, 110, 114, 118, 122, 126, 130) showed antioxidant activity higher than the simple sum (B) of the antioxidant activity of each individual extract that constituted them.

Among these, in the case of Example 98 including extracts of deciduous tree, prickly lotus, and pink needle flower, the antioxidant value was increased by a whopping 45.71% compared to the simple sum of the antioxidant value of the same concentration of deciduous tree extract alone (Example 95), the antioxidant value of the prickly lotus extract alone (Example 96), and the antioxidant value of the pink needle flower extract alone (Example 97), showing a remarkably excellent effect.

3) Comparison of each extract alone and with four combinations

The results of evaluating and comparing the antioxidant activity of each of the extracts of the common maple, the extracts of the prickly lotus, the extracts of the rock stone flower, and the extracts of the pink needle, alone and in combination of four, are as follows.

Phellodendron
(ug/mL) Thorn lotus
(ug/mL) Rockflower
(ug/mL) Pink Needleflower
(ug/mL) Antioxidant capacity (%)
(A) Simple sum of antioxidant activity of individual extracts
(B) Comparison of antioxidant activity values of combined extracts (A) and simple sum of antioxidant activity of individual extracts (B) Example 131 1.87 - - - 8.56 46.27 The antioxidant capacity of the combined extract (Example 135) increased by 7.47% Example 132 - 1.87 - - 6.42 Example 133 - - 1.87 - 26.41 Example 134 - - - 1.87 4.88 Example 135 1.87 1.87 1.87 1.87 50.00 - Example 136 0.93 - - - 3.98 22.65 The antioxidant capacity of the combined extract (Example 140) increased by 16.09% Example 137 - 0.93 - - 1.87 Example 138 - - 0.93 - 14.43 Example 139 - - - 0.93 2.37 Example 140 0.93 0.93 0.93 0.93 27.00 - Example 141 1.72 - - - 7.86 44.67 The antioxidant capacity of the combined extract (Example 145) increased by 10.66% Example 142 - 3.45 - - 14.15 Example 143 - - 0.86 - 13.53 Example 144 - - - 3.45 9.14 Example 145 1.72 3.45 0.86 3.45 50.00 - Example 146 0.86 - - - 3.63 21.86 The antioxidant capacity of the combined extract (Example 150) increased by 13.02% Example 147 - 1.72 - - 5.73 Example 148 - - 0.43 - 7.99 Example 149 - - - 1.72 4.50 Example 150 0.86 1.72 0.43 1.72 25.13 -

As a result of the experiment, it was confirmed that all examples had antioxidant activity. In addition, even though the same concentration was used, it was confirmed that all examples (Examples 135, 140, 145, 150) containing the combined extract showed antioxidant activity higher than the simple sum (B) of the antioxidant activity of each individual extract that constituted it.

Among these, in the case of Example 140 including aloe extract, aloe vera extract, aloe vera flower extract, and aloe vera extract, the antioxidant value was increased by 16.09% compared to the simple sum of the antioxidant value of the aloe extract alone (Example 136), the aloe vera flower extract alone (Example 137), the aloe vera flower extract alone (Example 138), and the aloe vera flower extract alone (Example 139) at the same concentration, showing a significantly superior effect.

In summary, in order to exhibit 50% antioxidant effect based on ABTS antioxidant activity, an average of 12.62 (ug/mL) was required when a single extract was used, but it was confirmed through the present invention that when two or more of the extracts of the Chinese yam tree, the extract of the prickly lotus, the extract of the rocky azalea, and the extract of the pink needle flower were used in combination, the same antioxidant activity could be achieved with only an average concentration of 7.92 (ug/mL). Through this, the composition of the present invention is advantageous in terms of not only excellent antioxidant activity but also economic aspects and ease of formulation application.

Although the embodiments of the present invention have been described above, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical idea or essential features thereof. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive.

Claims (10)

Containing at least one selected from among Juglans mandshurica extract, Euyale ferox extract, and Epilobium angustifolium extract and Rhodiola rosea extract as active ingredients, or
An antioxidant cosmetic composition comprising, as active ingredients, at least one selected from Euyale ferox extract, Rhodiola rosea extract, and Epilobium angustifolium extract and Juglans mandshurica extract.
delete In the first paragraph,
An antioxidant cosmetic composition characterized in that the Juglans mandshurica extract is an extract of at least one part selected from the group consisting of a stem, a leaf, a fruit, a flower, and a root, the Euyale ferox extract is an extract of at least one part selected from the group consisting of a whole plant, a leaf, a fruit, a flower, and a root, the Rhodiola rosea extract is an extract of at least one part selected from the group consisting of a whole plant, a stem, a leaf, a fruit, a flower, and a root, and the Epilobium angustifolium extract is an extract of at least one part selected from the group consisting of a whole plant, a stem, a leaf, a fruit, a flower, and a root.
In the first paragraph,
An antioxidant cosmetic composition, characterized in that the extract is an extract extracted using at least one solvent selected from the group consisting of water, methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, and glycerin.
In the first paragraph,
An antioxidant cosmetic composition, characterized in that the extract is an extract extracted by at least one method selected from a reflux extraction method, a hot water extraction method, a hot water extraction method, an immersion extraction method, and an ultrasonic extraction method.
In the first paragraph,
An antioxidant cosmetic composition characterized in that the extract is extracted by hot water extraction at 50-130°C for 5 minutes to 10 hours or by immersing in an organic solvent at 5-40°C for 6 hours to 5 days.
In the first paragraph,
An antioxidant cosmetic composition characterized in that the above extract has ABTS radical scavenging ability.
In the first paragraph,
An antioxidant cosmetic composition, characterized in that each of the above extracts is independently included in an amount of 0.001 to 25 wt% based on the total weight of the cosmetic composition.
In the first paragraph,
An antioxidant cosmetic composition characterized in that when two or more extracts from the Juglans mandshurica extract, the Euyale ferox extract, the Rhodiola rosea extract, and the Epilobium angustifolium extract are included, the respective extracts are included in a weight ratio of 0.5 to 1.5: 0.5 to 2: 0.1 to 2: 0.5 to 2.
In the first paragraph,
An antioxidant cosmetic composition characterized in that the cosmetic composition has at least one formulation selected from a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray.