KR102416803B1 - 배치식 살균과 후살균 공정을 이용한 상온유통 스파우트 파우치 포장 식물성 발효 유산균 음료 개발 - Google Patents
배치식 살균과 후살균 공정을 이용한 상온유통 스파우트 파우치 포장 식물성 발효 유산균 음료 개발 Download PDFInfo
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- KR102416803B1 KR102416803B1 KR1020210183410A KR20210183410A KR102416803B1 KR 102416803 B1 KR102416803 B1 KR 102416803B1 KR 1020210183410 A KR1020210183410 A KR 1020210183410A KR 20210183410 A KR20210183410 A KR 20210183410A KR 102416803 B1 KR102416803 B1 KR 102416803B1
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- Prior art keywords
- lactic acid
- acid bacteria
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- beverage
- prepared
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 336
- 241000894006 Bacteria Species 0.000 title claims abstract description 171
- 239000004310 lactic acid Substances 0.000 title claims abstract description 168
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 168
- 235000013361 beverage Nutrition 0.000 title claims abstract description 65
- 238000009826 distribution Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 18
- 235000021121 fermented vegetables Nutrition 0.000 title abstract description 4
- 230000008569 process Effects 0.000 title abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 title description 14
- 239000000843 powder Substances 0.000 claims abstract description 81
- 235000004347 Perilla Nutrition 0.000 claims abstract description 71
- 241000229722 Perilla <angiosperm> Species 0.000 claims abstract description 71
- 235000019985 fermented beverage Nutrition 0.000 claims abstract description 57
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 32
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 32
- 229920000881 Modified starch Polymers 0.000 claims abstract description 32
- 239000004368 Modified starch Substances 0.000 claims abstract description 32
- 239000006071 cream Substances 0.000 claims abstract description 32
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims abstract description 32
- 229940107187 fructooligosaccharide Drugs 0.000 claims abstract description 32
- 235000019426 modified starch Nutrition 0.000 claims abstract description 32
- 229920001277 pectin Polymers 0.000 claims abstract description 32
- 239000001814 pectin Substances 0.000 claims abstract description 32
- 235000010987 pectin Nutrition 0.000 claims abstract description 32
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 32
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 32
- 239000000203 mixture Substances 0.000 claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 claims abstract description 19
- 239000004615 ingredient Substances 0.000 claims abstract description 18
- 238000010298 pulverizing process Methods 0.000 claims abstract description 15
- 238000010025 steaming Methods 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 235000013311 vegetables Nutrition 0.000 claims description 59
- 239000000463 material Substances 0.000 claims description 30
- 241001300674 Plukenetia volubilis Species 0.000 claims description 18
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 15
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 14
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 14
- 238000011081 inoculation Methods 0.000 claims description 3
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims 9
- 235000018102 proteins Nutrition 0.000 abstract description 15
- 239000000796 flavoring agent Substances 0.000 abstract description 11
- 235000019634 flavors Nutrition 0.000 abstract description 11
- 238000002156 mixing Methods 0.000 abstract description 8
- 240000006677 Vicia faba Species 0.000 abstract 2
- 235000010749 Vicia faba Nutrition 0.000 abstract 2
- 235000002098 Vicia faba var. major Nutrition 0.000 abstract 2
- 230000000052 comparative effect Effects 0.000 description 20
- 238000002360 preparation method Methods 0.000 description 19
- 230000001953 sensory effect Effects 0.000 description 15
- 238000003860 storage Methods 0.000 description 15
- 235000015140 cultured milk Nutrition 0.000 description 12
- 229930006000 Sucrose Natural products 0.000 description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 9
- 239000008213 purified water Substances 0.000 description 9
- 230000008859 change Effects 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 241000234282 Allium Species 0.000 description 4
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000005022 packaging material Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000186660 Lactobacillus Species 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 229940039696 lactobacillus Drugs 0.000 description 3
- 235000013618 yogurt Nutrition 0.000 description 3
- 230000001093 anti-cancer Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000014048 cultured milk product Nutrition 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000008821 health effect Effects 0.000 description 2
- 238000007603 infrared drying Methods 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000020185 raw untreated milk Nutrition 0.000 description 2
- 240000002234 Allium sativum Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 235000021102 Greek yogurt Nutrition 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 238000009920 food preservation Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 235000021109 kimchi Nutrition 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000007395 thrombosis prophylaxis Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
- A23L5/13—General methods of cooking foods, e.g. by roasting or frying using water or steam
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/502—Gums
- A23V2250/5072—Pectine, pectinate
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/31—Mechanical treatment
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/38—Multiple-step
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
| 보관기간 (주) |
pH | 결과 | ||
| 냉장 (5℃) |
상온 (15~25℃) |
미온 (35℃) |
||
| 0 | 4.45 | 4.45 | 4.45 | 적합 |
| 1 | 4.45 | 4.45 | 4.45 | 적합 |
| 2 | 4.45 | 4.45 | 4.44 | 적합 |
| 3 | 4.45 | 4.45 | 4.44 | 적합 |
| 4 | 4.45 | 4.45 | 4.44 | 적합 |
| 5 | 4.45 | 4.45 | 4.43 | 적합 |
| 6 | 4.45 | 4.45 | 4.43 | 적합 |
| 7 | 4.45 | 4.45 | 4.43 | 적합 |
| 8 | 4.45 | 4.45 | 4.40 | 적합 |
| 9 | 4.45 | 4.45 | 4.40 | 적합 |
| 10 | 4.45 | 4.43 | 4.38 | 적합 |
| 11 | 4.45 | 4.40 | 4.36 | 적합 |
| 12 | 4.45 | 4.40 | 4.33 | 적합 |
| 13 | 4.45 | 4.38 | 4.33 | 적합 |
| 14 | 4.45 | 4.35 | 4.33 | 적합 |
| 15 | 4.45 | 4.33 | 4.30 | 적합 |
| 16 | 4.45 | 4.33 | 4.30 | 적합 |
| 보관기간 (주) |
관능검사(5점척도) | 결과 | ||
| 냉장 (5℃) |
상온 (15~25℃) |
미온 (35℃) |
||
| 0 | 3.6 | 3.6 | 3.6 | 적합 |
| 1 | 3.7 | 3.7 | 3.4 | 적합 |
| 2 | 3.7 | 3.6 | 3.4 | 적합 |
| 3 | 3.7 | 3.7 | 3.4 | 적합 |
| 4 | 3.8 | 3.6 | 3.3 | 적합 |
| 5 | 3.7 | 3.6 | 3.4 | 적합 |
| 6 | 3.6 | 3.5 | 3.2 | 적합 |
| 7 | 3.4 | 3.4 | 3.2 | 적합 |
| 8 | 3.5 | 3.5 | 3.2 | 적합 |
| 9 | 3.5 | 3.3 | 3.2 | 적합 |
| 10 | 3.5 | 3.3 | 3.3 | 적합 |
| 11 | 3.6 | 3.3 | 3.2 | 적합 |
| 12 | 3.5 | 3.3 | 3.1 | 적합 |
| 13 | 3.5 | 3.2 | 3.1 | 적합 |
| 14 | 3.5 | 3.2 | 3.0 | 적합 |
| 15 | 3.5 | 3.2 | 3.0 | 적합 |
| 16 | 3.5 | 3.3 | 3.0 | 적합 |
| 보관기간 (월) |
세균수(cfu/mL) | 유산균수,대장균군(cfu/mL) | 결과 | ||||
| 냉장 (5℃) |
상온 (15~25℃) |
미온 (35℃) |
냉장 (5℃) |
상온 (15~25℃) |
미온 (35℃) |
||
| 0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 1 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 2 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 3 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 4 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 5 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 6 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 7 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 8 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 9 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 10 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 11 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 12 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 13 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 14 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 15 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,1,0 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 16 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,1,1 | 0,0,0,0,0 | 0,0,0,0,0 | 0,0,0,0,0 | 적합 |
| 종류 | 단맛 | 풍미 | 이미, 이취 | 선호도 |
| 제조예 1 | 2.8 | 4.0 | 1.4 | 4.2 |
| 제조예 2 | 2.8 | 4.5 | 1.0 | 4.6 |
| 비교예 1 | 2.6 | 3.0 | 3.4 | 2.8 |
| 비교예 2 | 2.7 | 3.6 | 2.6 | 3.7 |
| 종류 | 단맛 | 풍미 | 이미, 이취 | 선호도 |
| 제조예 1 | 2.8 | 4.0 | 1.4 | 4.2 |
| 제조예 2 | 2.8 | 4.5 | 1.0 | 4.6 |
| 비교예 3 | 2.7 | 3.7 | 1.8 | 3.9 |
| 비교예 4 | 2.8 | 3.8 | 1.2 | 4.0 |
| 재료 종류 | 제조예 1 | 비교예 5 | 비교예 6 |
| 자소엽 분말 | 0.1 | - | 0.5 |
| 코코넛크림 | 28 | 32 | 24 |
| 백설탕 | 6.6 | 3 | 10 |
| 프락토올리고당 | 5 | 10 | 2 |
| 변성전분 | 0.5 | 0.2 | 0.9 |
| 파바빈프로테인 | 2.2 | 3 | 1 |
| 펙틴 | 0.4 | 0.1 | 0.7 |
| 식물성 유산균 | 0.0025 | 0.0025 | 0.0025 |
| 유산균 사균체 | 0.005 | 0.005 | 0.005 |
| 정제수 | 57.1925 | 51.6925 | 60.8925 |
| 종류 | 단맛 | 풍미 | 이미, 이취 | 선호도 |
| 제조예 1 | 2.8 | 4.0 | 1.4 | 4.2 |
| 비교예 5 | 2.4 | 3.3 | 2.0 | 3.4 |
| 비교예 6 | 2.3 | 3.5 | 1.8 | 3.6 |
Claims (7)
- (1) 자소엽을 90~110℃에서 5~15분 동안 증숙한 후, 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주를 접종하여 35~40℃에서 30~40시간 동안 발효한 후 50~60℃에서 20~28시간 동안 건조하고 분쇄하여 자소엽 분말을 제조하는 단계;
(2) 음료 재료 총 중량 기준으로, 상기 (1)단계의 제조한 자소엽 분말 0.08~0.12 중량%와 코코넛크림 26~30 중량%, 설탕 5~8 중량%, 프락토올리고당 4~6 중량%, 변성전분 0.4~0.6 중량%, 파바빈프로테인 2~2.4 중량%, 펙틴 0.3~0.5 중량%, 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주 0.002~0.003 중량%, 락토바실러스 파라카제이(Lactobacillus paracasei) 균주 0.004~0.006 중량% 및 물 55~59 중량%의 음료 재료를 준비하는 단계;
(3) 상기 (2)단계의 준비한 자소엽 분말, 코코넛크림, 설탕, 프락토올리고당, 변성전분, 파바빈프로테인, 펙틴, 락토바실러스 파라카제이(Lactobacillus paracasei) 균주 및 물을 혼합한 혼합물을 80~90℃에서 25~30분 동안 살균하는 단계:
(4) 상기 (3)단계의 살균한 혼합물에 상기 (2)단계의 준비한 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주를 접종한 후 35~40℃에서 30~40시간 동안 배양하여 유산균 발효 음료를 제조하는 단계; 및
(5) 상기 (4)단계의 제조한 유산균 발효 음료를 85~90℃에서 8~12분 동안 후살균하는 단계를 포함하여 제조하는 것을 특징으로 하는 상온유통용 식물성 발효 유산균 음료의 제조방법. - 제1항에 있어서, 상기 (2)단계의 음료 재료 준비 시 사차인치 분말 및 눈개승마 분말을 추가로 준비하는 것을 특징으로 하는 상온유통용 식물성 발효 유산균 음료의 제조방법.
- 삭제
- 삭제
- 제2항에 있어서, 상기 음료 재료는 음료 재료 총 중량 기준으로, 자소엽 분말 0.08~0.12 중량%, 사차인치 분말 0.08~0.12 중량%, 눈개승마 분말 0.08~0.12 중량%, 코코넛크림 26~30 중량%, 설탕 5~8 중량%, 프락토올리고당 4~6 중량%, 변성전분 0.4~0.6 중량%, 파바빈프로테인 2~2.4 중량%, 펙틴 0.3~0.5 중량%, 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주 0.002~0.003 중량%, 락토바실러스 파라카제이(Lactobacillus paracasei) 균주 0.004~0.006 중량% 및 물 55~59 중량%의 음료 재료를 준비하는 것을 특징으로 하는 상온유통용 식물성 발효 유산균 음료의 제조방법.
- 제5항에 있어서,
(1) 자소엽을 90~110℃에서 5~15분 동안 증숙한 후, 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주를 접종하여 35~40℃에서 30~40시간 동안 발효한 후 50~60℃에서 20~28시간 동안 건조하고 분쇄하여 자소엽 분말을 제조하는 단계;
(2) 음료 재료 총 중량 기준으로, 상기 (1)단계의 제조한 자소엽 분말 0.08~0.12 중량%와 사차인치 분말 0.08~0.12 중량%, 눈개승마 분말 0.08~0.12 중량%, 코코넛크림 26~30 중량%, 설탕 5~8 중량%, 프락토올리고당 4~6 중량%, 변성전분 0.4~0.6 중량%, 파바빈프로테인 2~2.4 중량%, 펙틴 0.3~0.5 중량%, 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주 0.002~0.003 중량%, 락토바실러스 파라카제이(Lactobacillus paracasei) 균주 0.004~0.006 중량% 및 물 55~59 중량%의 음료 재료를 준비하는 단계;
(3) 상기 (2)단계의 준비한 자소엽 분말, 사차인치 분말, 눈개승마 분말, 코코넛크림, 설탕, 프락토올리고당, 변성전분, 파바빈프로테인, 펙틴, 락토바실러스 파라카제이(Lactobacillus paracasei) 균주 및 물을 혼합한 혼합물을 80~90℃에서 25~30분 동안 살균하는 단계:
(4) 상기 (3)단계의 살균한 혼합물에 상기 (2)단계의 준비한 스트렙토코커스 써모필러스(Streptococcus thermophilus) 및 락토바실러스 델브루엑키 아종 불가리쿠스(Lactobacillus delbrueckii subsp. bulgaricus)의 혼합 균주를 접종한 후 35~40℃에서 30~40시간 동안 배양하여 유산균 발효 음료를 제조하는 단계; 및
(5) 상기 (4)단계의 제조한 유산균 발효 음료를 85~90℃에서 8~12분 동안 후살균하는 단계를 포함하여 제조하는 것을 특징으로 하는 상온유통용 식물성 발효 유산균 음료의 제조방법. - 제1항, 제2항, 제5항, 제6항 중 어느 한 항의 방법으로 제조된 상온유통용 식물성 발효 유산균 음료.
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