KR100816266B1 - A cosmetic composition for improving and alleviating atopic dermatitis, comprising a stabilized mung bean extract and red ginseng extract, which are entrapped in a nanoliposome containing pseudoceramide. - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving and alleviating atopic dermatitis, comprising a stabilized mung bean extract and red ginseng extract, which are entrapped in a nanoliposome containing pseudoceramide. The composition of the present invention improves skin penetration and skin moisturizing ability by entrapping and stabilizing pseudoceramide similar to ceramide, which is one of the intercellular lipid components, in nanoliposomes, and includes green and green ginseng extracts having excellent anti-inflammatory and antibacterial effects. By improving the function, it is effective in improving and alleviating atopic dermatitis.

Description

Cosmetic composition for improving and alleviating atopic dermatitis, containing mung bean extract and red ginseng extract stabilized in nanoliposomes containing pseudoceramides as active ingredients in Nanoliposome Comprising Pseudoceramide as Active Ingredient}

The present invention relates to a cosmetic composition for improving and alleviating atopic dermatitis

The stratum corneum of the skin acts as a protective barrier against external stimuli and foreign substance invasion, and maintains moisture in the skin through intercellular lipids. The major constituents of intercellular lipids are ceramides (40-50%), cholesterol (20-25%) and free fatty acids (20-25%). Decreasing or increasing the concentration of any of the three major lipids results in loss of protective barrier function of the skin.

Among them, ceramide is the most important lipid of intercellular lipids, and is well known as a component that prevents water evaporation and maintains water. Reduction of ceramide production by genetic factors or aging decreases the protective barrier function of the stratum corneum, and thus it is reported that various dermatological symptoms such as atopic dermatitis and psoriasis symptoms (Fulmer & Kramer, J. Invest) Derm ., 86: 598-602 (1986); and Tupker RA et al., Acta Derm.Vereereol Stockh, 70: 1-5 (1990)).

Atopic Dermatitis, on the other hand, is a chronic recurrent eczema with genetic predisposition, and its cause and pathogenesis are not clearly identified. Although several external environmental factors related to atopic dermatitis exacerbation are well known, genetic predisposition, and the genetic identification according to clinical and immunological forms, are currently weak. Previous studies on atopic dermatitis have been conducted only on the allergic side, but now, non-allergic aspects of atopic dermatitis such as eczema, sebum secretion, dry skin and abnormal skin vascular reactions have become important factors.

In general, patients with atopic dermatitis suffer from worsening water retention and loss of skin barrier function compared to the general population, which causes the symptoms to become more severe due to infection with secondary bacterial infectious agents. This causes skin dryness, pruritus, inflammation, and erythema, and the like, for the treatment of such symptoms, a method of strengthening the intercellular lipid membranes (such as essential fatty acids and moisturizers) and anti-inflammatory substances and antibacterial substances that inhibit inflammation. Etc. are used. In particular, ceramides cause a significant decrease in the amount of atopic dermatitis, leading to dry skin, which results in loss of the protective function of the skin surface and invasion of foreign substances, leading to secondary infections. Will be raised.

Because of its excellent moisturizing function, ceramide is used to improve and alleviate atopic dermatitis. Natural ceramides have excellent moisturizing capabilities, but they are limited in their low solubility and cost. Therefore, many researchers have studied how to effectively prepare synthetic ceramides, and the present inventors have also synthesized pseudoceramides very similar to natural ceramides, and have been used in cosmetics in the form of multiple lamellar emulsions containing pseudoceramides (Korean Patent Registration No. 539965). It has been confirmed that it shows an excellent effect on dermatitis, such as atopic dermatitis.

On the other hand, anti-inflammatory and antibacterial ingredients are one of the ingredients necessary to relieve atopic symptoms as well as skin barrier improvement effect using ceramide. The mung bean extract obtained by our supercritical fluid extraction method has anti-inflammatory and anti-pruritic functions and is known to be safe for humans even after long-term use (development of mung bean extract with improved atopy and relieving effect and cosmetic composition using it: Korean patent Application No. 2003-94625), in the case of the ginseng extract is known as an antimicrobial component that continuously removes contaminated bacteria without side effects in order to suppress secondary bacterial growth associated with atopic dermatitis.

Accordingly, the present inventors have attempted to develop a cosmetic composition exhibiting an atopic improvement and alleviation effect with enhanced anti-inflammatory, anti-pruritic and antibacterial functions while maintaining the effect of the functional raw material of nanoliposomes containing pseudoceramide.

The present inventors have completed the present invention by confirming that the cosmetic composition containing synthetic pseudoceramide having a moisturizing effect, mung bean extract having an anti-inflammatory effect, and a ginseng extract having an antibacterial effect has an effect of improving and alleviating atopic dermatitis. .

Therefore, an object of the present invention is to provide a cosmetic composition for improving and alleviating atopic dermatitis, containing mung bean extract and red ginseng extract stabilized by inclusion in a nanoliposome containing pseudoceramide.

In order to achieve the above object, the present invention provides a cosmetic composition for improving and alleviating atopic dermatitis, containing a stabilized mung bean extract and gourd extract extracted in nanoliposomes containing pseudoceramide as an active ingredient.

In the cosmetic composition of the present invention, the active ingredient exhibiting atopic dermatitis alleviation and improvement effect has anti-inflammatory, anti-pruritic and antibacterial effects on nanoliposomes having essential ceramides, cholesterol, and lecithin as essential ingredients. It was stabilized by inclusion of the indicated ginseng extract and mung bean extract.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1-100 nm in the form of conventional liposomes. The term "liposome" mentioned above is a colloidal particle arranged and reciprocally spontaneous combination of particles composed of amphiphilic molecules with water-soluble heads and insoluble tails, having a double-membrane structure similar to a cell membrane, and the composition containing the damaged skin It can be expected to restore the barrier, strengthen the skin barrier, and alleviate skin irritation. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposomes is 30-70nm, when the average particle diameter of the nanoliposomes exceeds 70nm, the improvement and formulation of the skin penetration among the technical effects to be achieved in the present invention The improvement in stability is very slight.

Nanoliposome of this invention is C _{12 -} comprises a saturated lipid between ₂₂ alkyl chain or unsaturated fatty acids and cells. The saturated or unsaturated fatty acids of the C _12-22 alkyl chains used in the preparation of the nanoliposomes of the present invention preferably include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid and palmilean acid More preferably stearic acid, palmilean acid and mixtures thereof. The amount used is 0.05-5.0% by weight, preferably 0.1-4.0% by weight relative to the total weight of nanoliposomes.

The intracellular lipids used for the preparation of the nanoliposomes are pseudoceramide: cholesterol: lecithin in a weight ratio of 0.1-5: 0.1-5: 0.1-2, more preferably 1-4: 1-4: 0.5-2, most preferably 2: 2: 1 by weight.

As used herein, the term "quasiceramide" refers to a chemical structure and efficacy similar to that of natural ceramide, an intercellular lipid component.

The pseudoceramide used as an intercellular lipid component in the cosmetic composition of the present invention is a compound represented by the following formula (I).

인 경우 Z는 -OH 이고, X는 -OH,

또는

이며; Z가

인 경우 Y는 -OH 이고, X는 -OH,

또는

이며; 그리고 상기 R은 C1-C40의 포화 또는 포화되지 않은 직쇄 또는 분쇄 지방족 탄화수소기를 나타내며, 치환된 경우에는 1개 이상의 수산기(-OH)를 갖는다.Y is

When Z is -OH, X is -OH,

or

Is; Z is

Where Y is -OH, X is -OH,

or

Is; And R represents a C1-C40 saturated or unsaturated straight or crushed aliphatic hydrocarbon group, and if substituted, has one or more hydroxyl groups (-OH).

The pseudoceramide compound of the present invention is invented by the present inventors and is disclosed in detail in PCT Patent Application No. PCT / KR01 / 01838.

In the present invention, the term "aliphatic hydrocarbon group" means all hydrocarbon groups (alkyl group, alkenyl group and alkynyl group) except aromatic hydrocarbon.

The term "alkyl group" in the present invention means a straight chain or branched saturated aliphatic hydrocarbon group having a specified carbon number, for example, alkyl is methyl, ethyl, propyl, iso-propyl, propenyl, butyl, iso-butyl, n -Pentyl, n-hexyl, nonyl, decyl, hexadecyl, eicosyl, triacontyl and tetracontyl.

As used herein, the term "alkenyl group" refers to a straight chain or branched saturated aliphatic hydrocarbon group having the specified carbon number, for example, alkenyl is ethenyl, propenyl, n-pentenyl, nonenyl, decenyl, hexa Deceenyl, eicosenyl, triacontenyl and tetraacontenyl.

The pseudoceramide compound of the present invention as described above functions as a moisture barrier, and this property, as a result, to improve the moisturizing power of the skin, there is an improvement and alleviation effect for atopic dermatitis.

The nanoliposomes of the present invention may further comprise emollients and preferably include caprylic / capric triglycerides. The content is 1.0-15.0 wt% with respect to the total weight of nanoliposomes, preferably 3.0-7.0 wt%.

The preparation of the nanoliposomes of the present invention can be made through various methods known in the art, but most preferably the mixture comprising the above components is prepared by applying a high pressure homogenizer. The preparation of nanoliposomes by the high pressure homogenizer can be carried out under various conditions depending on the desired particle size, and is preferably prepared by passing the high pressure homogenizer 1-5 times under 600-1200 bar pressure.

The content of the nanoliposomes of the present invention is 0.1-20.0% by weight, more preferably 1.0-10.0% by weight relative to the total weight of the cosmetic composition of the present invention.

Mung bean ( Phaseolus radiatus , Mung Bean, Green Bean, 綠 豆) used as an active ingredient in the cosmetic composition of the present invention is a perennial herb belonging to the dicotyledonous rosewood legume, widely distributed in Asia. Mung bean extract is extracted from the seeds of mung beans, the main components are proteins and fats, and has a strong antipyretic, detoxification action, is widely used in herbal medicine. According to the prescriptions related to skin beauty in the literature such as Dongbobogam, 'Okyongseosi', which is used like soap, makes the face look like jade, 'Yeooksan', which is effective for removing dry skin and removes blemishes, Oksan, and 'Oknaeyoung', which removes sweat bands that occur frequently in summer, are used. In addition, mung beans have been used effectively in yellow spear (bushrum), carbuncle (boil) and mumps (pandemic mumps) caused by dermal filamentous fungi in the stratum corneum.

Mung bean extract of the present invention can be extracted using a variety of extraction methods known in the art, but preferably can be obtained using supercritical fluid extraction (supercritical fluid extraction). The supercritical fluid extraction method effectively extracts fat-soluble substances by using supercritical carbon dioxide. After extracting fat-soluble substances, the supercritical carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction container. It may cause components that were not extracted to be extracted.

As a more specific embodiment, the preparation method of mung bean extract is as follows: after washing the mung bean with purified water, drying it and crushing it into small pieces, the sample is placed in a supercritical extraction vessel and pure carbon dioxide is transferred to a suitable temperature using a liquid chromatography pump. And the extract is received in a test tube while flowing into a supercritical state under pressure.

The supercritical fluid extraction method of the present invention is preferably carried out using carbon dioxide at a temperature of 35 to 100 ° C. and 100 to 500 atmospheres, more preferably at a temperature of 50 to 70 ° C. and 200 to 400 atmospheres, most preferably Is carried out using carbon dioxide at 60 ° C. and 300 atm.

The supercritical fluid extraction method is carried out using a mixed fluid in which co-solvents are additionally mixed with carbon dioxide. The co-solvent may be selected from the group consisting of alcohol, squalene, butylene glycol or a mixture of alcohol and squalene. Preferably a 1: 1 mixture of alcohol and squalene.

The mixed fluid contains 3 to 40% by volume, most preferably 10% by volume, based on the total volume. In addition, the extract of the present invention includes not only the extract by the above-described extraction method, but also an extract that has undergone a conventional purification process. Mung bean extract of the present invention may be prepared in a powder state by an additional process such as vacuum distillation, freeze drying or spray drying.

According to a preferred embodiment of the present invention, the mung bean extract is included as 0.001-40% by weight, more preferably 0.1-25% by weight relative to the total weight of nanoliposomes.

The ginseng ( Sophora flavescens ) used as another active ingredient in the cosmetic composition of the present invention is a perennial herb of the dicotyledonous rosewood legume, also known as the cane of the thief, the ginseng and the sperm of the snake. Roots from the skin of red ginseng are well known as ingredients for preventing fungal growth, moisturizing, whitening and improving blood circulation as well as preservative effects. Ginseng is effective for jaundice, dysentery, crayfish, and itch caused by moist heat of the lower part.Itching is used for washing the affected area with decoction, itching, pustules, scabies and ringworms, and piss Effective for not seeing sick symptoms

The term 'ginseng extract' in the present invention means that obtained by extracting from various organs or parts (such as leaves, flowers, roots, stems, branches, shells and fruits, etc.) of the ginseng, preferably stems, branches, bark or Roots, more preferably branches, bark or roots, most preferably extracts from the roots.

The ginseng extract in the present invention can be obtained through various methods known in the art, preferably (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol) , Butanol, and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol as an extraction solvent. Preferred extraction solvents are water, ethanol and methanol, even more preferably a mixture of water and ethanol, most preferably 70% ethanol. Suitable amounts of the extraction solvent are 3 to 10 times the dry ginseng dry weight, preferably 4 to 8 times, most preferably 6 times.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also the ginseng extract having substantially the same effect using other extraction solvents.

The ginseng extract of the present invention is extracted by a process comprising the following steps: (a) 3 to 10 times water, 1-4 anhydrous or hydrous lower alcohols, acetone, ethyl acetate, butyl acetate and 1 to red ginseng Adding a solvent selected from the group consisting of 3 butylene glycol; (b) refluxing the solvent added ginseng of step (a) to heat extraction for 4 to 6 hours; And (c) filtering and drying the extracted components.

According to a specific embodiment of the present invention, the above-described extraction process is carried out as follows: First, the washed, dried and broken shredded ginseng is placed in 70% ethanol for 5 hours in an extractor equipped with a cooling condenser. Extract by heating at 90 ° C. and filtering with 300 mesh. After aging at 5-10 ° C. for 7-10 days, it is filtered through Whatman No. 5 filter paper. This filtrate is obtained by drying with a rotary vacuum evaporator at 65 ° C.

In addition, the ginseng extract of the present invention includes not only the extract by the above-described extraction method, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the ginseng extract of the present invention.

The ginseng extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

According to a preferred embodiment of the present invention, the ginseng extract is included as 0.001-40% by weight, more preferably 0.1-25% by weight relative to the total weight of nanoliposomes.

According to a preferred embodiment of the present invention, the atopic dermatitis improvement and alleviation cosmetics containing mung bean extract and red ginseng extract stabilized in a nanoliposome containing pseudoceramide as an active ingredient, compared to each of the components and mixtures of the components Excellent improvement of damaged skin barrier recovery (Examples 3 and 5), skin moisturizing (Examples 4 and 6), anti-inflammatory (Examples 5 and 7) and atopic dermatitis (Examples 6 and 9) .

The following test examples and examples describe the content of the present invention, but the content of the present invention is not limited thereto.

Preparation Example 1 Preparation of Mung Bean Extract Using Supercritical Fluid Extraction Method

Mung beans are washed with purified water, dried and crushed into small pieces, and then 100 grams of sample is placed in a supercritical fluid extractor (Jasco, Japan) and used with a liquid chromatography pump (Supercritical fluid chromatograph, Jasco, Japan). Using pure carbon dioxide and cosolvent (a mixture of spirit solvent, a polar solvent, and squalene, a non-polar solvent, in a 1: 1 ratio), making it into a supercritical state at a temperature of 80 ° C. and a pressure of 300 atm for 40 minutes every 4 minutes. The extract was received in a 10 ml glass test tube. The extract was dried using a rotary evaporator, and then dried mung bean extract powder was used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 1 Preparation of Ginseng Extract

The roots of red ginseng are washed, dried and crushed into small pieces. 200 grams of the sample is placed in 1.2 liters of 70% ethanol, extracted by heating at 70-90 ° C. for 5 hours in an extractor equipped with a cooling condenser and filtered through 300 mesh. After aging at 5-10 ° C. for 7-10 days, the resultant was filtered using Whatman No. 5 filter paper. The filtrate was dried at 65 ° C. with a rotary vacuum evaporator to obtain a dry weight of 10.58 g.

Example  One. Pseudoceramide  Contains mung bean extract and Gosam  Extracts In a hurry  Stabilized Of nanoliposomes  Produce

To prepare a nano liposome with the composition of Table 1.

Preparation of Nanoliposomes (Content: wt%) Prize ingredient Test Example 1 Test Example 2 Test Example 3 Test Example 4 Test Example 5 Test Example 6 Test Example 7 Test Example 8 Test Example 9 Test Example 10 A glycerin 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 B Lecithin 5.0 5.0 5.0 5.0 10.0 10.0 10.0 5.0 5.0 5.0 cholesterol 5.0 5.0 10.0 10.0 5.0 5.0 10.0 10.0 10.0 10.0 Pseudoceramide 5.0 10.0 5.0 10.0 5.0 10.0 10.0 10.0 10.0 10.0 Stearic acid 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Caprylic / Capric Triglycerides 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 C Mung Bean Extract (10%) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 0.0 0.0 Red ginseng extract (10%) 20.0 20.0 20.0 20.0 20.0 20.0 20.0 0.0 20.0 0.0 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

인 경우, Z가 -OH이고, X가 -OH인 유사세라마이드를 사용하였으며, 이 유사세라마이드의 합성법은 PCT/KR01/01838호에 자세히 나와 있다.Phase B was added to A phase and uniformly wetted, then C phase was added to AB phase, heated and mixed to 70 ° C., and stirred uniformly. Subsequently, the mixture was passed through a high pressure homogenizer 1-5 times under conditions of 600-1200 bar and cooled to prepare nanoliposomes having a particle size of 30-70 nm in diameter. The pseudoceramide used at this time is Y in the compound of Formula 1,

In the case of, Z is -OH and X is -OH pseudoceramide was used, the synthesis method of this pseudoceramide is described in detail in PCT / KR01 / 01838.

Example 2. Stability Test of Nanoliposomes

The stability test for the nanoliposomes prepared in Example 1 was carried out by the following method.

The nanoliposome of Test Example 1-10 was placed in an opaque glass container and stored for 2 weeks in a thermostat maintained at 45 ° C., and also kept in a opaque glass container kept constant at 4 ° C. for 2 weeks in a completely shaded refrigerator. After storage, the degree of recrystallization was measured, and the degree of recrystallization was classified according to the grade of Table 2. The results are shown in Table 3 below.

Product discoloration evaluation criteria score Evaluation standard 0 No change One Very little crystallization 2 Little crystallization 3 A little bit severe crystallization 4 Badly crystallized 5 Extremely Crystallized

Crystallization Degree of Nanoliposomes Temperature Test Example 1 Test Example 2 Test Example 3 Test Example 4 Test Example 5 Test Example 6 Test Example 7 Test Example 8 Test Example 9 Test Example 10 45 ℃ 3 One 2 0 2 2 2 0 0 0 4 ℃ 4 2 2 0 3 3 4 0 0 0

As can be seen from the results of Table 3, it can be seen that the nanoliposomes (test examples 4, 8, 9 and 10) having the ratio of pseudoceramide: cholesterol: lecithin have a weight ratio of 2: 2: 1. there was. In addition, the stability was maintained even if a large amount of mung bean extract or gosam extract is contained.

Example 3 Recovery Effect After Skin Barrier Injury in Hairless Mice

In order to measure the recovery effect after damage of the skin barrier of nanoliposomes, which include all the pseudo-ceramides, mung bean extracts, and ginseng extracts, which are the active ingredients, the creams and extracts containing the nanoliposomes including pseudoceramides, mung bean extracts, and ginseng extracts as shown in Table 4 Cream containing nanoliposomes not included was prepared. Comparative Example 4 is a case in which all the raw materials contained in the nanoliposomes in the cream formulation containing no nanoliposomes are added to the formulation as raw materials instead of nanoliposomes.

Preparation of Nanoliposomal Cream (Content: Weight%) ingredient Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Nanoliposomes (Test Example 4) 10.0 - - - - Nanoliposomes (Test Example 8) - 10.0 - - - Nanoliposomes (Test Example 9) - - 10.0 - - Nanoliposomes (Test Example 10) - - - 10.0 - Lecithin - - - - 0.5 cholesterol - - - - 1.0 Pseudoceramide - - - - 1.0 Stearic acid - - - - 0.2 Caprylic / Capric Triglycerides - - - - 0.5 glycerin - - - - 1.5 Mung Bean Extract - - 2.0 2.0 2.0 Red ginseng extract - 2.0 - 2.0 2.0 Hydrogenated Lecithin 2.0 2.0 2.0 2.0 2.0 Grape Seed Oil 10.0 10.0 10.0 10.0 10.0 Camellia oil 5.0 5.0 5.0 5.0 5.0 Tocopheryl Acetate 0.2 0.2 0.2 0.2 0.2 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 Glyceryl Caprylate 0.3 0.3 0.3 0.3 0.3 Purified water To 100 To 100 To 100 To 100 To 100

In order to find out the recovery power after the skin damage of the cosmetics prepared above, the following experiment was carried out. Acetone was applied as a method of skin barrier damage. When transepidermal water loss (TEWL) reaches 4.0 g / m ² / h by dropping acetone into the embryos of 8-12 week old hairless mice, the examples of Table 4 and Comparative Examples 1-4 Samples were applied in an area of 5 cm ² . TEWL was measured by Tewameter TM210, a water evaporator manufactured by CK (Courage + Khazaka, Cologne, Germany). After 6 hours of application, TEWL was measured to evaluate the extent to which TEWL was reduced to assess the extent to which skin barrier function was restored. Recovery rate calculation used in the evaluation was calculated as in Equation 1 below, the results are shown in Table 5.

Br (Barrier Recovery) = {1- (B _{t = 6} -B _{t = 0} ) / (B _{t = d} -B _{t = 0} )} ㅧ 100

B _{t = 6} : TEWL measured 6 hours after skin barrier injury

B _{t = 0} : TEWL measurement before skin barrier injury

B _{t = d} : TEWL measured immediately after skin barrier injury

Skin moisture content after 6 hours (Initial TEWL) Application Sample Skin moisture content (g / m ² / h) Example 68.5 Comparative Example 1 62.5 Comparative Example 2 63.1 Comparative Example 3 60.8 Comparative Example 4 12.4

Through the results of Table 5, the cosmetic composition of Examples, Comparative Examples 1, 2 and 3 containing pseudoceramide-containing nanoliposomes after the skin barrier damage compared to the cosmetic composition of Comparative Example 4 does not contain nanoliposomes Was found to be very excellent. The presence of extract did not significantly affect the recovery effect after skin barrier damage.

Example  4. Moisture skin through human experiment Moisturizing  evaluation

In order to evaluate the moisturizing power of each cosmetic composition including nanoliposomes stabilized by inclusion of mung bean extract and red ginseng extract, which contained pseudoceramide, water retention capacity was measured as follows.

In a constant temperature and humidity room of 23 ° C. and 45% relative humidity, the cosmetics of Examples and Comparative Examples 1, 2, 3, and 4 were coated with a predetermined amount (0.03 g / 16 cm ² ) in the lower half of 30 test subjects, and then, 2, After 4 and 8 hours, the change in skin conductivity was measured to evaluate the moisturizing effect of the skin. The amount of water changed compared to the initial value was calculated as in Equation 2 below, and the results are shown in Table 6. The measuring device used a moisture content meter (corneometer CM 820, Courage + Khazaka, Cologne, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin.

Moisture Growth M (Moisturization) = {(M _{t = i} -M _{t = 0} ) / M _{t = 0} } * 100

M _{t = i} : Moisture content measured at t = i hours

M _{t = 0} : Moisture measured immediately before the start of the test at the unapplied area (t = 0).

Skin moisture growth rate over time (%) division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 After 2 hours 105 99 97 96 44 After 4 hours 95 90 89 90 25 After 8 hours 77 75 76 74 14

Looking at the results of the table, Examples, Comparative Examples 1, 2 and 3 of the cosmetics showed a significantly superior skin moisture increase effect compared to the cosmetics of Comparative Example 4, and among them, the effect of the cosmetic of the embodiment was the most excellent.

Example 5 Anti-inflammatory Effect Assessment Method-Carrageenan Foot Edema

In order to confirm that the cosmetic of the present invention exhibits an excellent anti-inflammatory effect, the cosmetics of Examples and Comparative Examples 1, 2, 3, and 4 were respectively administered to the mouse abdominal cavity at a dose of 10-100 mg / kg. One hour after administration, 0.5 ml of 0.1% carrageenan solution was injected into the rear paw of the experimental animal to induce inflammation. The change in the paw volume of the rat immediately after carrageenan injection and 4 hours after the injection was measured, and the edema inhibition rate was calculated according to the following Equation 3, and the results are shown in Table 7.

Edema inhibition rate (%) = {1-(ΔV treatment / ΔV control)} ㅧ 100

 Where ΔV is the volume change of the foot.

Edema inhibition rate (%) Dose (mg) Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 10 20.3 16.5 12.0 10.6 2.6 50 57.8 48.8 39.6 22.3 10.8 100 60.9 55.3 43.8 37.6 11.8

Through the results in Table 7, it can be seen that the cosmetic composition of the Example is superior to the anti-inflammatory effect compared to the comparative example. In addition, Comparative Example 1, in which the Mung bean extract is contained in the nanoliposomes, it can be seen that the anti-inflammatory effect is superior to other comparative examples containing just the Mung Bean extract.

Example 6. Atopic Improvement

A total of 100 patients (46 males, 54 females) comprised of 1 to 20 years of age with atopic symptoms were divided into 5 groups of 20 people, three times daily for the cosmetics of Examples, Comparative Examples 1,2,3, and 4. It was applied for 4 weeks and evaluated. The evaluation items were conducted by a panel test through a questionnaire. The scale of the panel test was determined according to Table 8, and the results are shown in Table 9.

Panel test scale Measure Evaluation standard 100% Full healing 50% Considerable healing 25% Slightly improved 0% No healing effect

Atopy Improvement over Time (Survey Average (%)) Cosmetics After 1 week After 2 weeks of use After 3 weeks of use After 4 weeks of use Example 44.6 60.4 73.2 88.9 Comparative Example 1 21.9 32.5 50.6 68.5 Comparative Example 2 20.5 32.6 45.7 66.2 Comparative Example 3 5.2 10.6 20.4 40.2 Comparative Example 4 2.5 3.3 4.1 5.0

As seen in Table 9, it was found that the cosmetics containing mung bean extract and red ginseng extract stabilized by inclusion in the nanoliposome containing pseudoceramide as an active ingredient have an excellent atopic dermatitis improvement and alleviation effect.

Based on the experimental example, the cosmetic composition of the various solubilizing agent type and the emulsifying agent type was used below, using the nanoliposomes of Test Example 4 including pseudoceramide having excellent atopy improvement and alleviating effect and including mung bean extract and red ginseng extract stabilized. . Although the formulation example explains the composition in detail, the composition of this invention is not limited only to the following example.

Formulation Example 1 Softener (Skin Lotion)

A flexible longevity containing a stabilized nanoliposome (Test Example 4) was prepared according to a conventional method, including pseudoceramide and encapsulating mung bean extract and red ginseng extract as shown in Table 10 below.

ingredient Content (% by weight) Nanoliposomes 1.0 glycerin 5.0 1,3-butylene glycol 3.0 PEG 1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Octicdodeceth-16 0.2 Polysorbate 20 0.2 incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 2. Converging Cosmetic Water

As shown in Table 11 below, convergent longevity containing a stabilized nanoliposome (Test Example 4) was prepared according to a conventional method, including pseudoceramide, and mung bean extract and red ginseng extract.

ingredient Content (% by weight) Nanoliposomes 1.0 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch Hagel Extract 2.0 Citric acid a very small amount incense 0.02 Uniside-U 13 0.01 Green # 13 0.001 Distilled water Remaining amount Sum 100

Formulation Example 3. Nutrients

As shown in Table 12 below, nutrient-containing longevity containing a stabilized nanoliposome (Test Example 4) was prepared according to a conventional method, including pseudoceramide, and mung bean extract and ginseng extract.

ingredient Content (% by weight) Nanoliposomes 3.0 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 incense 0.01 Uniside-U 13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 4. Nutrition Cream

A nutrition cream containing a stabilized nanoliposome (Test Example 4) containing pseudoceramide and containing green bean extract and red ginseng extract as shown in Table 13 below was prepared according to a conventional method.

ingredient Content (% by weight) Nanoliposomes 10.0 Lipophilic monostearate 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Cured Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 5. Massage Cream

A massage cream containing a stabilized nanoliposome (Test Example 4) was prepared according to a conventional method, including pseudoceramide and encapsulating mung bean extract and red ginseng extract as shown in Table 14 below.

ingredient Content (% by weight) Nanoliposomes 1.0 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl Alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 incense 0.01 Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

Formulation Example 6. Essence

As shown in Table 15 below, the essence was prepared according to a conventional method including a stabilized nanoliposome (Test Example 4) containing pseudoceramide and containing mung bean extract and red ginseng extract.

ingredient Content (% by weight) Nanoliposomes 2.0 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 incense 0.02 Uniside-U 13 0.01 Green # 3 0.001 Distilled water Remaining amount Sum 100

Formulation Example 7 Pack

As shown in Table 16 below, a pack containing a stabilized nanoliposome (Test Example 4) containing pseudoceramide and containing mung bean extract and red ginseng extract was prepared according to a conventional method.

ingredient Content (% by weight) Nanoliposomes 2.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Chicdestrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 incense a very small amount Uniside-Y13 0.02 Green # 3 0.003 Distilled water Remaining amount Sum 100

As described in detail above, the present invention provides a cosmetic composition for improving and alleviating atopic dermatitis, which is contained in a nanoliposome containing pseudoceramide and is stabilized as an active ingredient. The cosmetic of the present invention exhibits excellent skin moisturizing effect and anti-inflammatory effect, and is very excellent in atopic dermatitis improvement effect.

As described above in detail specific parts of the present invention, it is apparent to those skilled in the art that these specific techniques are only preferred embodiments, and thus the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (8)

A cosmetic composition for improving and alleviating atopic dermatitis containing mung bean extract and red ginseng extract stabilized by inclusion in a nanoliposome including pseudoceramide represented by the following formula (I) as an active ingredient.

[Formula 1] In the above formula, Y is

When Z is -OH, X is -OH,

or

Is; Z is

Where Y is -OH, X is -OH,

or

Is; And R represents a C1-C40 saturated or unsaturated straight or crushed aliphatic hydrocarbon group, and if substituted, has one or more hydroxyl groups (-OH). The cosmetic composition of claim 1, wherein the nanoliposomes further comprise (i) intercellular lipids comprising cholesterol and lecithin and (ii) saturated or unsaturated fatty acids of C ₁₂ -C ₂₂ alkyl chains. The cosmetic composition according to claim 2, wherein the saturated or unsaturated fatty acid of the C ₁₂ -C ₂₂ alkyl chain is included in an amount of 0.1-1.0 wt% based on the total weight of the nano liposome. 3. The cosmetic composition according to claim 2, wherein the intercellular lipid has a pseudoceramide: cholesterol: lecithin in a weight ratio of 0.1-5: 0.1-5: 0.1-2. The cosmetic composition according to claim 1, wherein the content of the nanoliposome is 0.1-20.0 wt% based on the total weight of the cosmetic composition. The method of claim 1, wherein the mung bean extract is prepared by a supercritical fluid extraction method using a mixed fluid in which supercritical carbon dioxide is mixed with alcohol, squalene, butylene glycol or a cosolvent selected from the group consisting of a mixture of alcohol and squalene. Cosmetic composition, characterized in that it comprises 0.001-40% by weight relative to the total weight of nanoliposomes. According to claim 1, The ginseng extract is extracted with an extraction solvent selected from the group consisting of water, methanol, ethanol, propanol, butanol, acetone, ethyl acetate, chloroform, 1,3-butylene glycol, nano liposome gross weight Cosmetic composition, characterized in that it comprises 0.001-40% by weight. The composition of claim 1 wherein the cosmetic composition consists of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Cosmetic composition, characterized in that it has a formulation selected from the group.