KR100698452B1 - Method of preparing ruminant protective choline - Google Patents

Abstract

The present invention immobilizes choline on a carrier to remove water, and then, first, encapsulates the water-removed particles with a hydrophobic substance, and the encapsulated particles have an iodine value of 1.0 or lower and a melting point of 57 ° C. or higher and a melting point of 40 ° C. or higher. The present invention relates to a method for preparing ruminant protective choline, which is encapsulated by ruminant microorganisms in the rumen by encapsulating the fatty acid alone or in combination of two or more thereof.

The present invention described above,

Fixing the concentrated liquid choline to a carrier such as silica, cellulose, starch, zeolite, and then removing water to prepare choline immobilized on the carrier,

Preparing a first encapsulated coated choline through an extruder after mixing and stirring a binder coating agent such as hardening oil having a biswax, PEG, iodine value of 1.0 or less and a melting point of 57 ° C. or higher in a mixer,

The primary encapsulated choline of 0.5 ~ 2.0mm of the primary encapsulated choline is selected and the iodine value is 1.0 or less by using a second encapsulation device such as a high speed mixer, ribbon mixer, reggae mixer, fluidized bed process machine, etc. There is no fear that macropores are produced by preparing double coated rumen protective choline by secondary encapsulation process using a cured oil having a melting point of 57 ° C. or higher and one or a combination of two or more fatty acids having a melting point of 40 ° C. or higher. It has the advantage of showing uniform shape and uniformity, and because it goes through the second coating process, there is no possibility that choline is distributed on the surface, so most of the added choline can maintain stability, and it is a refrigerant used in the frozen spray method. The production cost of the product is low because there is less manufacturing cost and less site and equipment cost Characterized in that it can be configured to lower the value.

Description

Development of Ruminally Protected Choline {Development of ruminally protected nutrients and their utilization for milk and beef enrichment}

The present invention relates to a method for preparing ruminant protective choline, and in detail, the choline is immobilized on a carrier to remove water, and then, the particles from which water is removed are first encapsulated with a hydrophobic substance, and the encapsulated particles are iodine value 1.0. The present invention relates to a method for producing ruminant protective choline, which can be protected from rumen microorganisms in the rumen by encapsulating the cured oil having a melting point of 57 ° C. or higher or a fatty acid having a melting point of 40 ° C. or higher alone or a combination of two or more thereof.

Choline requirements for lactating cows are not yet known and are known as an essential ingredient in some livestock studies, but because most of the choline is broken down in the rumen of ruminants, it can be absorbed and used in the small intestine through the rumen. Development of protection technology is necessary.

The analogy to choline is similar to that of the essential amino acid methionine, which is a sign of deficiency, including muscle weakness, fatty liver development, and renal hemorrhage.

Studies with choline infusion confirmed that choline was a limiting nutrient in milking cows. Fatty liver development during choline deficiency occurred within a few days when cattle were fed choline deficiency feed, a mechanism of liver fat metabolism and secretion. Due to the abnormality of, and increased choline fatty acid transport was increased, it was effective in reducing the occurrence of fatty liver.

Choline supplementation has been reported to affect blood glucose, plasma cholesterol, serum insulin and serum NEFAs (choline supplementation). Choline supplementation affects milk production, milk composition, weight gain and carcass characteristics but is supplemented with feed. It has been reported that choline chloride breaks down much of the rumen.

Ruminant microorganisms have been reported to break down phosphatidyl choline from feed into choline and phosphodiglycerides. Ruminant microorganisms convert free choline and available choline to methane via an intermediate metabolite, trimethylamine, and choline is phosphatidyl by rumen protozoa. It has been reported that less than 1% of choline in the feed reaches the small intestine, which is used for the synthesis of -choline.

For this reason, it was recognized that there is a need for coated choline compounds that are stable to ruminant degradation, which can also be supplied to ruminants.

Choline has been devised to overcome the choline component from rumination for a long time because it is essential nutrients for livestock animals, but ruminants are not resolved in rumen.

For example, U.S. Patent 5.807.594 provides a choline compound for the purpose of increasing the weight of ruminants. However, various raw materials used as encapsulating agents are mentioned. .

U.S. Patent 5.496.571 is known to feed the encapsulated choline compounds to lactating cows, while U.S. Patent 5.190.775 states that feeding bioactive substances such as choline through hydrophobic coatings can prevent degradation in the rumen. There is no mention of a specific manufacturing method and there is a limit to the complete protection against degradation of the active substance by rumen microorganisms in the rumen by simply coating or encapsulating with a hydrophobic substance.

World patent WO 94/15480 mentions the encapsulation of choline compounds in ruminants to increase milk yield, but this also likewise uses hydrophobic coatings and is not specifically mentioned for their preparation.

World Patent WO 96/08168 mentions the preparation of a liquid choline compound by immobilizing a liquid choline compound and coating it with fatty acids or fatty acid salts. There are disadvantages.

 In addition, WO 03/059088 A1 is coated with lipids so as to be stable in the rumen, and US 2005/0019413 A1 mentions double coating, but suggests a complicated process.

In addition, many patents have been applied for the purpose of gaining weight and increasing milk production, and most commonly refer to the coating of a specific protective material to prevent the breakdown of choline in the rumen. It is not.

In addition, the commercially available ruminant protective choline, there is a product of the method of refrigeration spray by mixing the choline with the protective material, but in this case, the site and equipment costs are high, and by using the very cold air of the temperature of the cold wind used is minus 60 ℃ Product production costs will increase.

In addition, because the product is produced by instant cooling, there is a possibility of forming macro pores in the product, and the operating conditions are difficult during production. In addition, the choline arranged on the surface of the prepared rumen protective choline can not protect because there was a disadvantage that the effect is inferior.

Accordingly, the present invention has been made in order to solve the conventional problems as described above, after fixing the choline to the carrier to remove the water, the water is first encapsulated with a hydrophobic material, the encapsulated particles iodine A method for preparing ruminant protective choline, which is encapsulated using a cured oil having a value of 1.0 or less and a melting point of 57 ° C. or higher, or a fatty acid having a melting point of 40 ° C. or higher alone, or a combination of two or more thereof, to be protected from rumen microorganisms in the rumen. Its purpose is to provide.

Method for producing the rumen protective choline of the present invention for achieving the above object,

Fixing the concentrated liquid choline to a carrier such as silica, cellulose, starch, zeolite, and then removing water to prepare choline immobilized on the carrier,

Preparing a first encapsulated coated choline through an extruder after mixing and stirring a binder coating agent such as hardening oil having a biswax, PEG, iodine value of 1.0 or less and a melting point of 57 ° C. or higher in a mixer,

The primary encapsulated choline of 0.5 ~ 2.0mm of the primary encapsulated choline is selected and the iodine value is 1.0 or less by using a second encapsulation device such as a high speed mixer, ribbon mixer, reggae mixer, fluidized bed process machine, etc. In the frozen-sprayed products commercialized by preparing double-coated ruminant protective choline by secondary encapsulation process using a cured oil having a melting point of 57 ° C. or higher and a fatty acid having a melting point of 40 ° C. or higher of one or a combination of two or more thereof. There is no risk of the formation of macropores, which have been pointed out as a disadvantage, and it has the advantage of having a uniform and uniform shape, and in the case of the product made by the frozen spray method, the choline located on the particle surface has the disadvantage of inferior stability in the rumen. Compared to the first coating product containing choline, the second coating process Since most of the choline added is not likely to be distributed on cotton, it has the advantage of maintaining stability, and since it does not use the refrigerant used in the refrigeration spray method, it has the advantage of low manufacturing cost and low site and equipment cost. The unit price can be lowered.

Because of this, feeding choline in an unprotected form in the rumen is less effective and wasteful. Therefore, by developing the ruminant protective choline to use in livestock specification to improve the nutrient utilization efficiency of ruminants to increase productivity, to increase the transition in livestock products to develop a feed additive that can produce functional livestock products.

Hereinafter, exemplary embodiments of the present invention will be described with reference to the accompanying drawings.

(Example 1)

Fixing the concentrated liquid choline to a carrier such as silica, cellulose, starch, zeolite, and then removing water to prepare choline immobilized on the carrier,

After mixing and stirring the above-mentioned immobilized choline in a binder such as biswax, PEG, iodine value of 1.0 or less, and a curing oil having a melting point of 57 ° C. or more, and a mixer such as a ribbon mixer, a general exchanger, a homomixer, and a V-type mixer, Preparing an encapsulated coated choline through an extruder such as a treader or a plotter,

When producing the above-mentioned primary encapsulated coated choline, the extruder, the extruder temperature of the plotter is 10 ℃ to 60 ℃.

The primary encapsulated choline of 0.5 ~ 2.0 ㎜ in the primary encapsulated choline is selected by using a second encapsulation device such as a high speed mixer, ribbon mixer, reggae mixer, fluidized bed process machine, hardened vegetable oil, hardened corn oil , Hardened cotton oil, hardened peanut oil, hardened palm kernel oil, hardened palm oil, hardened palm stearin oil, hardened sunflower oil, hardened vegetable oil, etc. The double encapsulated rumen protective choline is prepared by secondary encapsulation processes using one or a combination of two or more fatty acids such as stearic acid.

(Example 2)

Process of preparing primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, and injecting it through a 1.0 mm porous plate;

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 3)

A process of preparing a primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher and injecting it through a 1.2 mm porous plate;

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 4)

delete

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 5)

Process of preparing primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and melting point of 57 ° C. or higher and injecting it through a 2.0 mm porous plate;

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 6)

Mixing 1 kg of monovalent soap with 5 kg of choline and injecting it through a 2.0 mm porous plate to prepare a primary capsule material,

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 7)

Mixing 1 kg of PEG with 5 kg of choline and injecting it through a 1.0 mm porous plate to prepare a primary capsule material,

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 8)

Mixing 1kg of Choline 5kg with 1kg of Tween 80 and injecting it through a 1.0mm porous plate to prepare a primary capsule material,

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 9)

Mixing 1 kg of Span 80 with 5 kg of Choline and injecting it through a 1.0 mm porous plate to produce a primary capsule material,

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

(Example 10)

Process of preparing primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, and injecting it through a 1.5 mm porous plate;

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or a fatty acid having a melting point of 40 ° C. or higher and a mixture of 500 g and a mixture thereof. To manufacture.

The double coating material described above is 33.3% of the total amount.

(Example 11)

A process of preparing a primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher and injecting it through a 1.2 mm porous plate;

1kg of the prepared primary capsule material into a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 350 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof by double coating. To manufacture.

The double coating material described above is 25.9% of the total amount.

(Example 12)

A process of preparing a primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher and injecting it through a 1.2 mm porous plate;

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or a 250 g of fatty acid having a melting point of 40 ° C. or higher and a mixture of 250 g of the mixture thereof. To manufacture.

The double coating material described above is 20.0% of the total amount.

(Example 13)

A process of preparing a primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher and injecting it through a 1.2 mm porous plate;

1 kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and melting point of 57 ° C. or higher, or 150 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof by double coating. To manufacture.

The double coating material described above is 13.0% of the total amount.

(Example 14)

Process of preparing primary capsule material by mixing 5 kg of choline with 1 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, and injecting it through a 1.0 mm porous plate;

1 kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or a fatty acid having a melting point of 40 ° C. or higher and 50 g of a mixture thereof by double coating. To manufacture.

The double coating material described above is 4.8% of the total amount.

(Example 15)

A process of preparing primary capsule material by mixing 4 kg of choline with 2 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, and injecting it through a 2.0 mm porous plate;

1 kg of the prepared primary capsule material is placed in a high speed mixer and double coated by adding 670 g of a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher and a fatty acid having a melting point of 40 ° C. or higher and 670 g of a mixture thereof. Do it.

The double coating material described above is 40.1% of the total amount.

(Example 16)

Process of preparing primary capsule material by mixing 3 kg of choline with 3 kg of hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher and injecting it through a 2.0 mm porous plate;

1kg of the prepared primary capsule material in a high speed mixer by adding a hardened oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or higher, or 670 g of a fatty acid having a melting point of 40 ° C. or higher and a mixture thereof 670 g by double coating. To manufacture.

The double coating material described above is 40.1% of the total amount.

The method of producing the rumen protective choline of the present invention configured as described above,

The concentrated liquid choline is immobilized on a carrier such as silica, cellulose, starch, zeolite and the like to remove water to prepare choline immobilized on the carrier.

After mixing and stirring the above-mentioned immobilized choline in a binder such as biswax, PEG, iodine value of 1.0 or less, and a curing oil having a melting point of 57 ° C. or more, and a mixer such as a ribbon mixer, a general exchanger, a homomixer, and a V-type mixer, The primary encapsulated coated choline is prepared through an extruder such as a treader, a floater, or the like.

When producing the above-mentioned primary encapsulated coated choline, the extruder, the extruder temperature of the plotter is 10 ℃ to 60 ℃.

The primary encapsulated choline of 0.5 ~ 2.0 ㎜ in the primary encapsulated choline is selected by using a second encapsulation device such as a high speed mixer, ribbon mixer, reggae mixer, fluidized bed process machine, hardened vegetable oil, hardened corn oil , Hardened cotton oil, hardened peanut oil, hardened palm kernel oil, hardened palm oil, hardened palm stearin oil, hardened sunflower oil, hardened vegetable oil, etc. The double encapsulated rumen protective choline is prepared by secondary encapsulation processes using one or a combination of two or more fatty acids such as stearic acid.

Looking at the in vitro loss rate according to the rumen protective treatment method for the double coated choline prepared by the above example it can be seen that as shown in Table 1 below.

Table 1

In addition, as a result of feeding the ruminant protective choline according to the present invention to ruminants, the milk component and the somatic cell number change are shown as shown in Table 2 below, and the milk component is increased and the somatic cell number is markedly decreased.

Table 2

On the other hand, in vitro tests and in vivo experiments were performed to measure the stability of the protective choline against water.

First, ruminant protective choline prepared for measuring stability in vitro was put in a test tube, and distilled water was added to prepare a 5% aqueous solution. The prepared aqueous solution was stirred at 40 ° C. ± 0.1 ° C. for 2 hours using a JEIO TECH MC-11 multisterer water bath.

Whatman paper NO. Filtering was carried out using 3 to take only solids and dried at 45 ° C. ± 0.1 ° C. for at least 12 hours to completely remove residual moisture.

After cooling the dried sample to room temperature in SANPLA DRY KEEPER, 300 mg of the chloroform and methanol in a volume ratio of 1: 1 mixed completely dissolved in 5% K ₂ CrO ₄ solution with 0.1N AgNO ₃ solution as an indicator Choline content was measured as a titration reagent, and repeated measurements were repeated three times in all assays.

In the in vitro test, a product having excellent stability, suitable product size, and high content of choline was selected and tested for stability in vivo.

The present invention is not limited by the above embodiments and may be used for the protection of various components that require rumen protection technology to feed ruminants.

As described in detail above, according to the method of preparing the rumen protective choline according to the present invention, by the method of preparing the double coated choline through the two-stage coating process and the choline supplementation, the rumen is not decomposed by microorganisms, pH, and water. It can be stably moved to the lower digestive tract and absorbed, and can be used for the specification of ruminant livestock to increase the nutrient utilization efficiency of ruminants, thereby increasing productivity.

In order to increase the nutrient availability of ruminants and to produce functional livestock products, the choline is treated in the rumen to allow the ruminants to adjust the amount according to their weight, productivity and purpose of use.

Feeding ruminants protects choline in the rumen, making it possible to efficiently add choline to feed, contribute to increased productivity, and produce choline-enriched functional livestock.

It can be applied to protect a variety of ingredients that need ruminant protection, and thus can be applied in various ways to produce new feed additives.

Claims (15)

Immobilizing the concentrated liquid choline compound on the immobilized carrier and then removing water to prepare choline immobilized on the immobilized carrier; Preparing a first encapsulated coated choline after mixing and stirring the immobilized choline in a binder coating agent and a mixer, and Among the primary encapsulated choline, 0.5 to 2.0 mm of primary encapsulated choline is screened and placed in a secondary encapsulation device, one of hardened oils having an iodine value of 1.0 or less and a melting point of 57 ° C. or higher or a fatty acid having a melting point of 40 ° C. or higher or their Method for producing a rumen protective choline, characterized in that configured to produce a double coated rumen protective choline by the process of secondary encapsulation by adding a combination of two or more. The method of claim 1, The concentrated liquid choline compound is choline chloride, choline dihydrogen citrate, tricholine citrate, choline bitartrate, or a method for producing a ruminant protective choline, characterized in that a mixture of two or more thereof. The method of claim 1, The immobilized carrier is a method of producing a ruminant protective choline, characterized in that the water insoluble carrier is silica, cellulose, starch, or zeolite. delete The method of claim 1, The binder coating agent is biswax; PEG; Surfactants; Divalent soaps which are calcium stearate, zinc stearate or magnesium stearate; Monovalent soaps which are sodium soaps or potassium soaps; Or hydrogenated oil having an iodine value of 1.0 or lower and a melting point of 57 ° C. or higher; Method of producing a ruminant protective choline, characterized in that one or a mixture of two or more thereof. The method of claim 5, The binding coating agent is a method of producing a ruminant protective choline, characterized in that the iodine value is 1.0 or less and the melting point of the melting point 57 ℃ or more. The method of claim 1, Cured oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or more is from cured vegetable oil, cured corn oil, cured cottonseed oil, cured peanut oil, cured palm kernel oil, cured palm oil, cured palm stearin oil, cured sunflower oil, or cured vegetable oil. A mixture of one or two or more selected, wherein the fatty acid having a melting point of 40 ° C. or more is one or two or more mixtures selected from fatty acids including palmitic acid and stearic acid. The method of claim 7, wherein Cured oil having a iodine value of 1.0 or less and a melting point of 57 ° C. or more is a cured palm stearin oil. The method of claim 1, The method of producing a ruminant protective choline, characterized in that the binding ratio of the choline and the binding coating agent is configured so that the binding coating agent per unit of choline 0.1 to 2 units. The method of claim 1, The mixer used when mixing the immobilized choline and the binding coating agent is a ribbon mixer, a general stirrer, a homo mixer, a V-type mixer, or a super mixer, the method of producing a rumen protective choline, characterized in that. The method of claim 1, Method for producing a rumen protective choline, characterized in that the extruder used when producing the primary encapsulated coated choline extruder or plotter. The method of claim 1, Method for producing a rumen protective choline, characterized in that the size of the injection-molded porous plate is 0.5mm to 2.5mm when producing the primary encapsulated coated choline. The method of claim 1, When manufacturing the primary encapsulated coated choline extruder extruder tube temperature is a method of producing a rumen protective choline, characterized in that configured to 10 ℃ ~ 60 ℃. The method of claim 1, The secondary encapsulation device is a high speed mixer, ribbon mixer, a reggae mixer, or a fluidized bed process machine, the method of producing a rumen protective choline, characterized in that. The method of claim 1, Method for producing a ruminant protective choline, characterized in that the content of the double coating is configured to be 15% to 55% of the total amount.