JPS60256375A - Bioreactor - Google Patents

Abstract

PURPOSE:To make it possible to exhibit the function of a reaction active element sufficiently in the stable state, by making easy and sure temperature control of a microorganism based reaction active element, e.g. an enzyme, immobilized at a position where the microorganism can be brought into contact with a reaction fluid. CONSTITUTION:A reaction fluid (A) is passed through the first path 7, and a heat medium (B) can be passed through the second path 8 adjacent to tha path 7 in a heat exchanger 1. A microorganism based reaction active element 3 which is an enzyme or a microbial cell capable of producing the enzyme is immobilized on plate fins 2 for heat exchange provided in the path 7. Thus, the temperature conditions of the microorganism based reaction active element 3, e.g. the enzyme, immobilized at a position where the microorganism can be brought into contact with the reaction fluid (A) can be easily and surely controlled, and the function of the above-mentioned reaction active element 3 can be exhibited sufficiently in the stable state.

Description

[Detailed description of the invention] (a) Industrial application field The present invention relates to a bioreactor that can be suitably used to promote various chemical reactions by utilizing the catalytic action of enzymes, fungi, etc. .

(b) Prior art In a conventional/halogen reactor, a tank-shaped container is filled with a column etc. in which enzymes or bacteria that produce the enzymes are immobilized, and a solution to be reacted is introduced into this container. It is common that the enzyme is brought into contact with the enzyme in sequence. However, with this type of structure, the temperature of the entire solution to be treated must be controlled in order to ensure the most suitable temperature for the enzyme to perform its catalytic action, but the temperature of the entire solution to be treated must be controlled. It is extremely difficult to maintain the optimum temperature because of the influence of As a result, the enzyme becomes hot and its activity deteriorates.

Undue fluctuations in temperature conditions can easily lead to variations in product quality.

(c) Purpose The present invention was made with attention to such circumstances,
It is possible to easily and reliably control the temperature conditions of microbial reaction active elements such as enzymes that are immobilized in positions where they can come into contact with the reaction fluid, and to maintain the functions of the reaction active elements in a sufficient and stable state. The purpose is to provide a bioreactor that can exhibit the following effects.

(D) Structure In order to achieve the above object, the present invention provides a heat exchanger configured to allow a reaction fluid to flow through a first passage and to allow a heat medium to flow through a second passage adjacent to the passage. The heat exchanger is characterized in that it includes a microbial reaction active element immobilized on a plate fin for heat exchange provided in the first passage of the heat exchanger.

(e) Examples An embodiment of the present invention will be described below with reference to the drawings.

FIG. 1 is a perspective view showing the entire bioreactor according to the present invention. This bioreactor comprises a heat exchanger 1 and a microbial reaction active element, such as an enzyme 3, immobilized on plate fins 2 of the heat exchanger 1. To be more specific, the heat exchanger 1 includes an aluminum partition plate 4,
The plate fin type is formed by alternately stacking and fixing plate fins 2 and 3, and for example, a suitable solution A as a reaction fluid is passed through the first passage 7 formed in an even number of stages and opening on one side. At the same time, the heat medium B can be allowed to flow through second passages 8 formed at odd-numbered stages and open to the other side. For example, as shown in FIG. 2, the plate fin 2.6 is made by pressing a single thin plate, and its upper surfaces 2a, 6a and lower surfaces 2b, 6b are rolled onto the partition plate 4. They are bonded by bonding or diffusion bonding. Note that a large number of regular micropores 9 are formed on the surface portion 2C of the plate fin 2 interposed in the first passage 7 by applying a porous anodic oxide film treatment (anodization treatment). Ru. The enzyme 3 is adsorbed and immobilized within these micropores 9. When performing porous anodic oxide film treatment, phosphoric acid or the like is used in combination with an electrolytic solution, whereby the diameter d of each micropore 9 is
For example, it is set to about 1100n.

With such a configuration, the solution A passing through the first passage 7 comes into contact with the enzyme 3 immobilized on the plate fin 2 in the passage 7.

Therefore, if enzymes 3 corresponding to the type of solution A and the reaction to be promoted are selected and defined in the plate fins 2, the function as a bioreactor will be exhibited. Moreover, in this device, the enzyme 3 is immobilized on the plate fin 2, which is most susceptible to the thermal influence of the heat medium B flowing through the second passage 8. Therefore, it is easy to maintain the temperature conditions of the enzyme 3 itself at an optimal value, and it is possible to maximize the catalytic action of the enzyme 3. Specifically, the heat generated when the enzyme 3 works can be taken out directly to the heat medium B side through the plate fins 2, so that even the enzyme 3, whose activity deteriorates at high temperatures, can be easily activated. Further, even if the quality of the product changes depending on the temperature conditions under which the enzyme 3 operates, the quality can be stabilized as long as the heat medium B at a constant temperature is allowed to flow through the second passage 8. In addition, if necessary, the activity temperature of enzyme 3,
It is also possible to change the temperature of the heat medium B easily and reliably.

Note that the microbial reaction active element to be immobilized on the plate fin is not limited to enzymes, and may be various types of fungi.

Furthermore, it goes without saying that the method for immobilizing the microbial reaction active element on the plate fin is not limited to the above method.
For example, by adhering or welding polymeric materials to the surface of plate fins, what about these polymeric materials? Alternatively, microbial reaction active elements such as enzymes may be immobilized.

Furthermore, the flow direction of the reaction fluid and heat medium in the heat exchanger may be parallel flow or cross flow (
Alternatively, the reaction fluid may be turned around.

(f) Effects Since the present invention has the configuration as described in 1- below, it is possible to easily and reliably control the temperature conditions of microbial reaction active elements such as enzymes immobilized at positions where they can come into contact with reaction fluids. It is possible to provide a bioreactor in which the functions of the reaction active elements can be fully and stably exhibited.

[Brief explanation of drawings]

The drawings show one embodiment of the present invention, with the first figure being a perspective view and the second figure being a perspective view.
The figure is an exploded perspective view, and FIG. 3 is an enlarged sectional view showing the surface portion of the plate fin. 1... Heat exchanger 21 @ plate fin 3... Microbial reaction active element (enzyme) 7... 1 First passage 81/Second passage A... Reaction fluid (solution. B...・Heating medium Figure 1 Figure 2 Figure 3

Claims (4)

[Claims] (1) A heat exchanger configured to allow a reaction fluid to flow through a first passage and a heat medium to flow through a second passage adjacent to the passage, and a heat exchanger provided in the first passage of the heat exchanger. A bioreactor comprising a heat exchange plate) and a microbial reaction active element immobilized on fins. (2) #The bioreactor according to claim 1, wherein the biological reaction active element is an enzyme or a bacterial cell that produces the enzyme. (3) Claim 1 or 2, characterized in that the microbial reaction active element is directly immobilized on the surface of the plate fin that has been treated with a porous anodic oxide film.
Bioreactor as described in Section. (4) #The bioreactor according to claim 1 or 2, wherein the biological reaction active element is adsorbed and fixed to a polymeric material attached to the surface of the plate fin.