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JPS6416586A - Production of baniii restriction enzyme - Google Patents

Production of baniii restriction enzyme

Info

Publication number
JPS6416586A
JPS6416586A JP62171747A JP17174787A JPS6416586A JP S6416586 A JPS6416586 A JP S6416586A JP 62171747 A JP62171747 A JP 62171747A JP 17174787 A JP17174787 A JP 17174787A JP S6416586 A JPS6416586 A JP S6416586A
Authority
JP
Japan
Prior art keywords
baniii
restriction endonuclease
plasmid
pban31
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62171747A
Other languages
Japanese (ja)
Other versions
JPH0630577B2 (en
Inventor
Fumikiyo Kawakami
Yoshihiko Maekawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP62171747A priority Critical patent/JPH0630577B2/en
Publication of JPS6416586A publication Critical patent/JPS6416586A/en
Publication of JPH0630577B2 publication Critical patent/JPH0630577B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To easily produce the titled substance in large quantity, by inserting a structural gene of a BanIII restriction endonuclease originated from Bacillus aneurinolyticus into a vector plasmid pBR322 and introducing the plasmid into E.coli. CONSTITUTION:A chromosome DNA containing BanIII restriction endonuclease gene originated from Bacillus aneurinolyticus IAM1077 is prepared and introduced into BamHI site of pBR322 to obtain a plasmid pBAN31 of 12kb. The plasmid is introduced into Escherichia coli HB101 strain to obtain a transformed Escherichia coli HB101 (pBAN31) (FERM P-9423). The strain is cultured in a nutrient medium to obtain BanIII restriction endonuclease without necessitating the removal of BanII restriction endonuclease.
JP62171747A 1987-07-09 1987-07-09 Ban III-Method for producing restriction endonuclease Expired - Fee Related JPH0630577B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62171747A JPH0630577B2 (en) 1987-07-09 1987-07-09 Ban III-Method for producing restriction endonuclease

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62171747A JPH0630577B2 (en) 1987-07-09 1987-07-09 Ban III-Method for producing restriction endonuclease

Publications (2)

Publication Number Publication Date
JPS6416586A true JPS6416586A (en) 1989-01-20
JPH0630577B2 JPH0630577B2 (en) 1994-04-27

Family

ID=15928940

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62171747A Expired - Fee Related JPH0630577B2 (en) 1987-07-09 1987-07-09 Ban III-Method for producing restriction endonuclease

Country Status (1)

Country Link
JP (1) JPH0630577B2 (en)

Also Published As

Publication number Publication date
JPH0630577B2 (en) 1994-04-27

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Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees