JPH11173987A - Microtiter viewer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a microtiter viewer whose light detecting efficiency is enhanced and whose light detection can be realized quickly and at a low cost. SOLUTION: The microtiter viewer is provided with a light source 2 which radiates excitation light used to excite a sample 9, a sample housing plate 6 which houses the sample 9 used to emit sample light when it is irradiated with the excitation light and in which a plurality of housing holes whose lower part is transparent are formed two-dimensionally, a two-dimensional photodetector 7 which is sensitive to the sample light and which can two-dimensionally decompose a position, a condensing part 5 which is interposed in a common optical path by an optical path between the sample housing plate 6 and the light source 2 and by an optical path between the sample housing plate 6 and the two-dimensional photodetector 7 and which comprises a plurality of lenses corresponding to the respective housing holes two-dimensionally, a light separation means 4 which is arranged in a common optical path between the excitation light and the sample light, by which the excitation light radiated from the light source 2 is made incident on the whole lower part of the condensing part 5 and by which the sample light radiated from the whole lower part of the condensing part 5 is made incident on the two-dimensional photodetector 7 and an output part 8 which outputs the signal of the two-dimensional photodetector 7.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microviewer for measuring changes in characteristics such as the amount of fluorescent light and changes in absorbance of a sample such as serum dispensed into a plurality of containers, and screening the sample. .

[0002]

2. Description of the Related Art Conventionally, a microtiter viewer using a microtiter plate for measuring a sample such as serum and cells has been known. This microtiter viewer irradiates the sample sample in the microtiter plate, into which various reagents are injected, with excitation light radiated from a light source, detects the light emitted from the sample by this irradiation with a light receiving element, and detects the detection result with the reagent. The screening of the sample sample is performed by comparing with a specified value according to the above, and the result of the screening is displayed.

As a technique related to such a microtiter viewer, for example, there is an automatic microplate spectroscopic analyzer disclosed in Japanese Patent Application Laid-Open No. 60-40955. This microplate spectrometer uses an optical fiber to simultaneously irradiate excitation light to sample samples in a plurality of sample sample receiving holes (hereinafter, referred to as “wells”), and absorbs and absorbs the fluorescence of the plurality of sample samples due to the irradiation. Are simultaneously detected using a detection fiber.

Another example is disclosed in Japanese Patent Application Laid-Open No. 9-4 / 1997.
There is a fluorescence detector disclosed in 3197 gazette. This fluorescence detection device is configured so that laser light emitted from a laser light source is sequentially incident on each well of a microtiter plate fixed on a stage, and fluorescence of a sample sample can be detected for each well. I have.

[0005]

However, the techniques related to the above-described microtiter viewer have the following problems.

First, in the automatic microplate spectroscopic analyzer disclosed in Japanese Patent Application Laid-Open No. 60-40955, an optical fiber for irradiating excitation light and an optical fiber for fluorescence detection are provided for each well. Although there is an advantage that fluorescence detection can be performed at the same time, there are drawbacks that a large number of optical fibers are required, which increases the cost and complicates the structure of the entire apparatus.

On the other hand, according to the fluorescence detector disclosed in Japanese Patent Application Laid-Open No. 9-43197, a laser beam can be applied to a sample without using an optical fiber, thereby reducing costs and simplifying the structure of the entire device. It is possible to However, in this fluorescence detection apparatus, the microtiter plate fixed on the stage is moved by the stage two-dimensional movable part, and the fluorescence is sequentially detected for each well, so that the fluorescence of the sample sample in a plurality of wells is simultaneously detected. It takes a lot of detection time compared to a device that can.

In the microtiter viewer, a microtiter plate is used as a device for accommodating a specimen sample. Recently, a microtiter plate having a large number of wells is used in order to increase the amount of a sample that can be detected at one time. I started. However, if the number of wells is increased without changing the outer diameter of the microtiter plate, the size of one well decreases, the amount of fluorescent light emitted from one well decreases, and the fluorescence detection becomes difficult. further,
The microtiter viewer is required to have a detection capability capable of detecting extremely weak light such as fluorescence emitted from a specimen sample, which was not detectable conventionally.

Accordingly, the present invention has been made to solve such a conventional problem, and provides a microtiter viewer capable of improving photodetection efficiency and realizing photodetection quickly and at low cost. The purpose is to do.

[0010]

In order to solve the above problems, a microtiter viewer of the present invention emits a sample light by irradiating excitation light with a light source for emitting excitation light for exciting a sample. A sample storage plate for storing a sample and having a plurality of two-dimensionally transparent storage holes at the bottom, a two-dimensional photodetector capable of two-dimensional position resolution having sensitivity to sample light, a sample storage plate, and a light source A light-collecting unit that is interposed on a common optical path of the optical path and the sample accommodation plate and the optical path of the two-dimensional photodetector, and has a plurality of two-dimensional lenses corresponding to each accommodation hole,
Arranged on the common optical path of the excitation light and the sample light, the excitation light emitted from the light source is made incident on the entire lower part of the light condensing part, and the sample light emitted from the entire lower part of the light condensing part is detected two-dimensionally. A light separating means for inputting the light to the detector and an output display unit for outputting a signal of the two-dimensional photodetector.

[0011] According to this microtiter viewer, the excitation light emitted from the light source is incident on the entire lower part of the light condensing part by the light separating means, and a plurality of excitation lights in all the accommodation holes provided in the sample accommodation plate are provided. The sample will be irradiated. Then, the sample light emitted from the plurality of samples by being irradiated with the excitation light can be simultaneously detected by the two-dimensional light detection unit, and the detection result is displayed on the output display unit. Therefore, detection of the sample light can be realized quickly and at low cost.

[0012] Further, it is possible to focus the excitation light incident by the light separating means on the sample precipitated in each of the receiving holes, by means of a light collecting section having a plurality of lenses corresponding to the respective receiving holes in a two-dimensional manner. As a result, the generation efficiency of the sample light is improved.
Furthermore, it is possible to condense the sample light to the two-dimensional photodetector by this condensing part, and the light detection efficiency in the two-dimensional photodetector is improved.

Preferably, the light separating means is a dichroic mirror that reflects the excitation light and transmits the sample light. If this dichroic mirror is used, the excitation light radiated from the light source can be reflected and efficiently incident on the entire lower part of the light collecting section. Further, the sample light emitted from the sample can be easily transmitted toward the two-dimensional photodetector.

It is also preferable that the light separating means is a dichroic mirror that reflects the sample light and transmits the excitation light. With this dichroic mirror,
Excitation light can be transmitted and efficiently incident on the entire lower part of the light-collecting portion. Further, the sample light is reflected and can be easily incident on the two-dimensional photodetector.

It is preferable that an excitation wavelength cut section for cutting the wavelength of the excitation light is provided on the optical path of the light separating means and the two-dimensional photodetector. If this excitation wavelength cut section is used, the excitation wavelength can be further cut, so that the S / N ratio of the two-dimensional photodetector can be increased and the sensitivity can be improved.

It is preferable that an excitation wavelength selector for selecting and transmitting the excitation light wavelength is provided on the optical path between the light source and the light separating means. By using the excitation wavelength selection unit, it is possible to select and transmit light of a wavelength that can excite the sample from the excitation light emitted from the light source, thereby improving the generation efficiency of the sample light and, consequently, the light detection efficiency. I can do it.

In addition, a field lens is provided on the optical path of the light source and the light separating means for inputting the excitation light as a parallel light beam toward the light separating means, and a focusing lens is provided on the optical path of the light separating means and the two-dimensional light detecting section. It is desirable to include a field lens that focuses a parallel light beam of the sample light emitted from the light unit on a two-dimensional photodetector. When the former field lens is used, all the accommodation holes can be irradiated perpendicularly to the sample accommodation plate, and the illumination unevenness is eliminated. Also, if the latter field lens is used, the light beam parallel to the optical axis of the sample light emitted from the specimen sample can be focused toward the two-dimensional photodetector, so that a large amount of sample light can be detected. , Shading can be eliminated.

[0018]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a preferred embodiment of a microtiter viewer according to the present invention will be described in detail.

First, the overall configuration of the microtiter viewer 1 according to the first embodiment of the present invention will be described with reference to FIG. The microtiter viewer according to the present embodiment is a device that measures the amount of fluorescence of a sample 9 such as serum and displays a screening result of the sample 9. The microtiter viewer mainly includes a mercury lamp 2 as a light source and an excitation wavelength selection unit. A certain excitation wavelength filter 3b, a dichroic mirror 4 as a light separating means, a microlens array plate 5 as a light condensing unit, a microtiter plate 6 as a sample holding plate,
CCD7 as a two-dimensional light detection unit, TV as an output display unit
It comprises a monitor 8 and a barrier filter 10 which is an excitation wavelength cut section.

As shown in FIG. 1, a dichroic mirror 4 for reflecting an excitation wavelength is disposed on an optical path between a mercury lamp 2 for emitting excitation light and a microtiter plate 6. The dichroic mirror 4 bends the optical axis A of the excitation light emitted from the mercury lamp 2 by 90 ° toward the microtiter plate 6 side to make the optical axis B, and makes the excitation light enter the entire lower part of the microtiter plate 6. It is installed to make it.

Here, as shown in FIG. 2, the specimen sample 9 is dispensed into a plurality of cylindrical wells 6a provided on the microtiter plate 6, and various reagents are injected into each well 6a. I have. The microtiter plate 6 has a total of 96 wells in 12 rows and 8 columns, and a transparent bottom plate 6b is fixed to the bottom surface. In addition, it is preferable to use a material that hardly emits fluorescent light for the transparent bottom plate 6b. For example, a non-fluorescent glass used for a microscope slide, a cover glass, or a fused silica glass is used.

As shown in FIG. 1, on the optical path of the dichroic mirror 4 and the mercury lamp 2, a condenser lens 3a and a projection lens 3c are arranged in this order from the mercury lamp 2 side. An excitation wavelength filter 3b for selectively transmitting the excitation light emitted from the mercury lamp 2 is disposed between the two condenser lenses 3a.

A microlens array plate 5 having a plurality of lenses arranged two-dimensionally is disposed immediately below the microtiter plate 6. Each lens of the microlens array plate 5 has a total of 96 lenses formed in 12 rows and 8 columns as well as the wells of the microtiter plate 6, and one lens is provided for each well.

As shown in FIG. 3, each lens of the microlens array plate 5 has a principal ray passing through the microlens at the center of the bottom of each well 6a because the specimen sample is deposited on the bottom of each well 6a. It is necessary to arrange so as to be incident. Therefore, the projection lens 3c to the well 6a
The distance to the center of the bottom surface of H (this distance is equal to the distance from the fluorescent image forming lens 11 to the well 6a),
If the distance from the bottom of the lens of the microlens array plate 5 to the bottom of the well 6a is d, and the distance from the center of the microtiter plate 6 to the center of the well 6a is L, the central axis of the microlens is: X = L It only has to be shifted inward from the center axis of the well 6a by d / H 2.

On the other hand, as shown in FIG. 1, below the dichroic mirror 4, on the extension of the optical axis B, as a two-dimensional photodetector for detecting the fluorescence emitted from the sample 9, a microtiter plate 6 is provided. A CCD 7 for picking up a fluorescent image at the bottom is provided. Further, on the optical path of the CCD 7 and the dichroic mirror 4, a barrier filter 10 for absorbing the excitation light and transmitting the fluorescent light, and a fluorescent image forming lens 1 for forming an image of the fluorescent light on the light receiving portion of the CCD 7.
1 is arranged.

As will be described later, the CCD 7 is connected to a computer 12 for integrating signal outputs for each well 6a and performing screening, and a TV monitor 8 for displaying a screening result of the sample 9.

Next, a method for measuring the amount of fluorescence of the sample sample by the microtiter viewer 1 of the present embodiment will be described with reference to FIG.

Excitation light emitted from the mercury lamp 2 is collected by the condenser lens 3a toward the projection lens 3c. At this time, since the excitation wavelength filter 3b is disposed between the condenser lenses 3a, only the wavelength that excites the sample 9 can be selected and transmitted. Then, the collected excitation light is projected toward the dichroic mirror 4 by the projection lens 3c.

The optical axis A of the excitation light projected on the dichroic mirror 4 is bent by 90 ° toward the microtiter plate 6 to become the optical axis B, and the excitation light is transmitted through the microlens array plate 5 and becomes microscopic. The light is incident on the entire lower part of the titer plate 6. Therefore, all the wells 6a can be irradiated with the excitation light at the same time, so that the speed of fluorescence detection can be increased. Also, JP-A-60-409
No need for an optical fiber for irradiating excitation light as in the automatic microplate spectrometer described in JP-A-55-55,
Cost reduction can be achieved.

In particular, when the excitation light is transmitted through the microlens array plate 5, each of the lenses 6a
Since the light is focused toward the sample accumulated at the bottom of the sample, the efficiency of irradiating the sample with the excitation light is greatly improved as compared with the case without a lens.

The sample irradiated with the excitation light emits fluorescence, and of the fluorescence, the light emitted from the bottom of each well is:
The light passes through the microlens array plate 5. Originally, fluorescence diverges in all directions, but in this embodiment, since the microlens array plate 5 is provided, the divergent fluorescence can be collected by each lens, and the fluorescence detection efficiency is greatly improved. improves. The fluorescent light travels along the same optical path as the excitation light toward the dichroic mirror 4 by the lens.

The fluorescence transmitted through the dichroic mirror 4 passes through a barrier filter 10 which is an excitation wavelength cut section. Since the barrier filter 10 absorbs the excitation wavelength, the excitation light radiated from the mercury lamp 2 or a very small amount of excitation light transmitted through the dichroic mirror 4 which is not transmitted after being reflected by the microtiter plate 6. Light can be further cut off. Then, the fluorescent light transmitted through the barrier filter 10 is irradiated onto the CCD 7 by the fluorescent imaging lens 11, and the CCD 7 can capture a fluorescent image of the bottom surface of the microtiter plate 6.

As described above, by using the microlens array plate 5 and the dichroic mirror 4, the fluorescence emitted from a plurality of samples can be simultaneously detected by the CCD 7, thereby speeding up the fluorescence detection. Further, an optical fiber or the like for light detection is not required, and cost can be reduced.

The signal output from each element of the CCD 7 proportional to the amount of fluorescent light is displayed on the TV monitor 8 as a video signal. In addition, the signal output is added by the computer 12 for each region of each well 6a, so that a fluorescent image of the bottom surface of the microtiter plate 6 can be more clearly captured. The signal output added for each region is used as an output signal of each well 6a, and this output signal and the sample sample 9 are output.
The screening of the specimen sample 9 is performed by comparing with a predetermined value corresponding to the reagent injected into the sample 9. This screening result is also displayed on the TV monitor 8. The screening can also be performed by observing the fluorescence of each sample on the screen of the TV monitor 8 with the naked eye instead of the computer 12. In addition, since the imaging result of the CCD 7 and the display on the TV monitor 8 are similarly two-dimensional with respect to the sample sample 9 injected two-dimensionally, screening is easily performed by assigning coordinates to each well. be able to.

Next, a second embodiment according to the present invention will be described with reference to FIG. In the microtiter viewer of the present embodiment, the bottom plate itself of the microtiter plate 30 is formed by a microlens array plate 31 made of a transparent and non-fluorescent material. According to the microtiter plate 30, the focal length of the microlens can be minimized, the incident angle (ωs ″) of the excitation light is maximized, the illuminance (E) of the excitation light of each well 6a and the CCD 7 are increased. The upper fluorescent illuminance (E ') indicates the maximum value.

Next, a third embodiment according to the present invention will be described with reference to FIG. The microtiter viewer of the present embodiment has a field lens 41 provided on the optical path of the projection lens 3c and the dichroic mirror 4, and a field lens 42 provided on the optical path of the microtiter plate 30 and the fluorescent image forming lens 11. . The focal length of the field lens 41 is f1, the focal point is arranged so as to coincide with the exit pupil of the projection lens 3c, the focal length of the field lens 42 is f2, and the focal point is the fluorescent image forming lens 1.
It is arranged so as to match one entrance pupil. The field lens 41 has a substantially flat surface on the projection lens 3c side and a convex surface on the dichroic mirror 4 side. The field lens 42 has a convex surface on the microtiter plate 30 side,
The fluorescent image forming lens 11 side is substantially flat.

The excitation light emitted from the light source is transmitted to the projection lens 3
The light is projected on the field lens 41 by c. The excitation light that has passed through the field lens 41 becomes light parallel to the optical axis A, is then reflected by the dichroic mirror 43, and is incident on the microtiter plate 30 in a state parallel to the optical axis B. Therefore, all the wells 30a are irradiated perpendicularly to the microtiter plate 30, and illumination unevenness is eliminated. Further, of the sample light emitted from the specimen sample 9, light parallel to the optical axis B is condensed on the fluorescent image forming lens 11 by the field lens 42, and then formed on the CCD 7. Therefore, fluorescence can be detected efficiently and shading can be eliminated.

Next, a fourth embodiment according to the present invention will be described with reference to FIG. The microtiter viewer 50 of the present embodiment is different from the microtiter viewer 1 of the first embodiment shown in FIG.
The position of the fluorescent imaging system such as CD7 is inverted.
In addition, a dichroic mirror 51 that transmits excitation light and reflects fluorescence is used as a light separating unit. That is, in the present embodiment, the excitation light emitted from the mercury lamp 2 passes through the dichroic mirror 51 and is incident on the entire lower surface of the microtiter plate 6, and the fluorescence emitted from the sample 9 is reflected by the dichroic mirror 51.
And is imaged on the CCD 7. Note that the microtiter viewer 50 according to the present embodiment depends on the wavelength of the excitation light.
And Microtiter Viewer 1
Fluorescence detection can be performed efficiently.

Next, the effect of providing the microlens array plate 5 in the present invention will be described using a comparison device 13 (unknown) of FIG. In the description of the drawings, the same elements as those of the microtiter viewer of FIG. 1 are denoted by the same reference numerals, and redundant description will be omitted.
When the excitation light is uniformly irradiated on the entire microtiter plate 6 by the comparison device, the excitation light illuminance (E) of each well becomes:
The luminance of the excitation light is B, and the angle of incidence of the excitation light on the well 6a is ω.
If s ′, then E = π · B · sin ² ωs ′.

Since the fluorescent luminance (B ') is proportional to the illuminance (E) of the excitation light, the fluorescent illuminance (E') on the CCD 7 is obtained.
Is given by E '= π · B' · sin ² ω '= π · (k · E) · sin ² ω' = k · π ² · B · sin ² ω '· sin ² ωs'. When the value of E ′ is large, the fluorescence detection efficiency is high. To increase the value of E ′, one of the excitation light luminance B, sin ² ω ′, and sin ² ωs ′ is increased. There is a need.

The value of the excitation light luminance B is an amount depending on the type of lamp, and hardly changes even if the wattage of the lamp is increased. Also, sin ω ′ is the brightness of the fluorescent image forming lens, and it is difficult to set it to 0.4 or more. on the other hand,
sinωs ′ is sinωs ′ = sinωs / β, where β is the magnification of the projection lens system. Since the projection lens is not an imaging system, sinωs
Can be about 0.7. However, since the size of the light source is small in the mercury lamp 2 or the like used as the excitation light source, the magnification β needs to be considerably large in order to irradiate the entire microtiter plate 6. Therefore, sinω
s 'is a very small value, and the fluorescence illuminance (E') on the CCD 7 is also very weak.

Therefore, it is almost impossible to increase the excitation light luminance B, sin ² ω ′, and sin ² ωs ′ in the comparison device 13 shown in FIG. In order to improve this point, the inventor of FIG.
Although the microlens array plate 5 is disposed directly below the microtiter plate 6 as shown in FIG.
s ″) can easily be made several times larger than the incident angle (ωs ′) of the comparison device 13. Each well 6a
Excitation light illuminance (E) and fluorescence illuminance on CCD 7 (E ')
Means si when there is no microlens array plate 5
n ² ωs ″ / sin ² ωs ′, which is a significant increase.

Next, the process of improving the light detection efficiency by providing the microlens array plate 5 will be described in detail with reference to FIG. (A) shows the case without the microlens array plate 5, and (b) shows the case with the microlens array plate 5. Preconditions are as follows. (1) Consider one well 20. (2) Fluorescent cells 21 as a sample are uniformly distributed on the bottom of a well at 1000
It is assumed that there are individual pieces. (3) Regardless of the presence or absence of the micro lens 22, it is assumed that the total luminous flux 23 entering one well does not change. (4) Assume that the incidence angle is increased by a factor of 10 by the lens (the number of irradiated cells is reduced to 1/100). (5) The fluorescence efficiency is 1/10. (6) When the microlens 22 is not provided, the light beam acquisition rate of the light receiving optical system (the ratio of the light beam entering the effective aperture of the light receiving optical system to the fluorescent light beams emitted from one cell in all directions) is 1/100.
Set to 0.

First, the case where there is no microlens array plate 5 will be described. Assuming that the total luminous flux 23 of the excitation light entering one well 20 is 1,000,000 (photons / second), from the precondition (2), one cell has 1,000 (photons / second).
Of the excitation light. The total amount of fluorescence 24 emitted from one cell is 100 (photons / second) from the precondition (5). From precondition (6), 1/1 of these
000, that is, 0.1 (photons / second) enters the CCD 7, so that the total amount of received light signals is 0.1 × 1000, and 100 (photons / second)
Becomes

Next, a case where the microlens array plate 5 is provided will be described. According to the precondition (3), the total luminous flux 23 of the excitation light entering one well is 1,000,000 (photon /
Second), and one cell is irradiated with 1,000 (photons / second) of excitation light. According to the precondition (4), if the incident angle of the excitation light is increased by a factor of 10, one cell will be irradiated with a squared multiple of that, that is, 1,000 × 100, 100,000 (photon / second) excitation light. The number of irradiated cells is 1/100, that is,
Only 10 pieces. The total luminous flux 24 from one cell is
From the precondition (5), it becomes 10,000 (photons / second), and among them, entering the CCD 7 is 1/10 of the precondition (6).
00 is further multiplied by 10 squared in consideration of the incident angle. Therefore, considering that the number of irradiated cells is 10, the total amount of received light signals is 10,000 (photons / second).
× (1/1000) × 100 × 10 pieces and 10,000
(Photons / second). This value is 100 times that of the case without the microlens, that is, the square of the irradiation angle magnification of 10 times, which indicates that the fluorescence detection efficiency is drastically increased.

When the microlens array plate 5 is not used, the image of the partition wall between the wells 6a appears on the CCD 7, and the area in which the fluorescent image can be integrated is reduced by that much. By using the microlens array plate 5 having no gap, the image of the partition wall portion is eliminated, and almost all the elements of the CCD 7 can be used as the fluorescent light output.

[0047]

As described above, according to the microtiter viewer of the present invention, the condensing section for condensing the excitation light toward the sample and condensing the sample light toward the two-dimensional photodetector is provided. The light detection efficiency can be improved because of the provision, and the light detection can be realized quickly and at low cost by providing the light separation means.

[Brief description of the drawings]

FIG. 1 is a diagram showing a first embodiment of a microtiter viewer according to the present invention.

FIG. 2 is a perspective view of a microtiter plate.

FIG. 3 is a diagram used to explain the position of a lens of a microlens array plate.

FIG. 4 is a diagram showing a second embodiment of the microtiter viewer according to the present invention.

FIG. 5 is a diagram showing a third embodiment of a microtiter viewer according to the present invention.

FIG. 6 is a diagram showing a fourth embodiment of a microtiter viewer according to the present invention.

FIG. 7 is a diagram showing a comparative device used for explaining the effect of the microlens array plate.

FIG. 8 is a diagram used to explain the effect of the microlens array plate in detail.

[Explanation of symbols]

1: Microtiter viewer, 2: Mercury lamp, 3b ...
Excitation wavelength selection unit, 4: dichroic mirror, 5: microlens array plate, 6: microtiter plate, 6a: well, 7: CCD, 9: sample sample.

Claims (7)

[Claims] 1. A light source that emits excitation light for exciting a sample, and a sample that emits sample light when irradiated with the excitation light is accommodated, and a plurality of accommodation holes having a transparent lower part are two-dimensionally arranged. A sample containing plate, a two-dimensional photodetector capable of two-dimensional position resolution having sensitivity to the sample light, and an optical path of the sample containing plate and the light source, and the sample containing plate and the two-dimensional photodetector. A light condensing unit that is interposed on a common optical path of the optical path and has a plurality of lenses two-dimensionally corresponding to each of the receiving holes, arranged on a common optical path of the excitation light and the sample light, and emitted from the light source. The excitation light is incident on the entire lower part of the light-collecting unit, and the sample light emitted from the entire lower part of the light-collecting unit is incident on the two-dimensional photodetector. And a release means, microtiter viewer, characterized by comprising an output display unit for outputting a signal of the two-dimensional photodetector. 2. The microtiter viewer according to claim 1, wherein the light separating means is a dichroic mirror that reflects the excitation light and transmits the sample light. 3. The microtiter viewer according to claim 1, wherein the light separating means is a dichroic mirror that reflects the sample light and transmits the excitation light. 4. The microtiter viewer according to claim 1, wherein a bottom portion of the sample storage plate constitutes the light collecting section. 5. An excitation wavelength cut section for cutting the wavelength of the excitation light is provided on an optical path of the light separating means and the two-dimensional photodetector. The microtiter viewer according to claim 1. 6. On the optical path of the light source and the light separating means,
The microtiter viewer according to any one of claims 1 to 5, further comprising an excitation wavelength selection unit that selects and transmits the excitation light wavelength. 7. On the optical path of the light source and the light separating means,
A field lens that enters the excitation light as a parallel light beam toward the light separation unit, and on the optical path of the light separation unit and the two-dimensional light detection unit, the sample light emitted from the light collection unit The microtiter viewer according to any one of claims 1 to 6, further comprising a field lens that focuses the parallel light beam on the two-dimensional photodetector.