JPH11108928A - Method for producing biopolymer array sheet - Google Patents

Abstract

(57) Abstract: A method for easily and reproducibly producing a sheet having a sequence of a biopolymer such as a nucleic acid and a protein. Kind Code: A1 A biopolymer is fixed on a linear support, arranged in a line, wound, and filled with a filler into a gap to form a rod. Thereafter, by slicing the rod thinly, an array sheet of biopolymers can be easily prepared.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for manufacturing a sheet prepared by arranging a plurality of prepared biopolymers in order to confirm the formation of a hybrid between biopolymers.

[0002]

2. Description of the Related Art Conventionally, a method for producing an array sheet of biopolymers is as follows. First, a polymer such as a nucleic acid or a protein, which is a material for preparing an array, is dropped onto a membrane (thin film) filter such as nylon for each type. It was manufactured by leaving, drying, or irradiating with ultraviolet rays as necessary so that each material was immobilized on the membrane.

In a DNA chip on which a fragment of deoxyribonucleic acid is immobilized, a method of selectively synthesizing a nucleic acid base sequence for each base using a photosensitive group on a wafer such as glass has been used. .

[0004]

However, in such a conventional method, a sample is dropped for each sequence or a single base sequence is synthesized using a photomask in order to prepare a sheet. However, there is a problem that it takes time to manufacture. In addition, there is a problem that the variation among the arrangement sheets is large.

[0005]

According to the present invention, in order to solve the above-mentioned problems, each array element is fixed to a linear support, and they are assembled into a desired shape. The bundle is sliced to produce a biopolymer array sheet.

[0006]

A plurality of biopolymer samples are adhered and fixed to different linear supports, respectively, and bundled. By slicing the bundled sample, a plurality of sheets can be easily manufactured with good reproducibility.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention will be specifically described below with reference to the drawings. FIG. 1 is a view showing a polymer array sheet according to one embodiment of the present invention. In FIG. 1, reference numeral 10 denotes an outer pipe, 11 denotes a capillary glass tube, 12 denotes a center pipe made of resin, 13 denotes a winding sheet, and 14 denotes a filler.

Here, the procedure for producing the polymer array sheet will be described with reference to FIG. First, a surface treatment for binding DNA is performed on the inner wall of all the capillary glass tubes 11, and DNA having a predetermined base sequence is bound to each of the capillary glass tubes 11. Further, as shown in FIG. 2, the capillary glass tube 11 into which DNA has been introduced is aligned on an adhesive take-up sheet 13.

The array of aligned capillary glass tubes is wound around a center pipe 12 as shown in FIG.
At the stage of winding all, the outer pipe 10 as shown in FIG.
And filling the gap with a filler 14 such as a two-liquid epoxy.
And cure. Here, the center of the center pipe and the center of the outer pipe are set to coincide.

[0010] The rod 3 is shaped like a rod
0 is shown in FIG. The rod 30 can be thinly sliced using a device such as a slicer (not shown) using a grindstone disk to produce a DNA sheet.

In this embodiment, the description has been made using a capillary glass tube. However, utilizing the fact that a positive charge is generated on the surface of a nylon fiber, DNA or protein is adsorbed and replaced with a capillary glass tube. Can be done. In addition, it is also possible to directly covalently bind DNA to the fiber and arrange it.

The filler is also hydroxyethyl methacrylate (Hydroxyethylmethacrylat).
By using a relatively soft material such as e) and using a flexible resin for the center pipe and the outer pipe, slicing can be easily performed.

In the present embodiment, the whole is sliced while being wound up in a circular shape. However, it is also possible to slicing the whole as a whole by overlapping and solidifying it while turning it right and left.

In this embodiment, the case where a nucleic acid, particularly a deoxyribonucleic acid, is used has been described. However, it is needless to say that a sheet can be produced by attaching proteins.

[0014]

As described above, according to the present invention, in the present invention, a biopolymer to be a sample is fixed to a rod-shaped support, and the bundle is cut and bundled, so that the polymer arrangement can be easily performed. Sheets can be manufactured.

[Brief description of the drawings]

FIG. 1 is an overall configuration diagram for explaining an embodiment of the present invention.

FIG. 2 is a diagram for explaining a method of bundling a support to which a biopolymer is attached.

FIG. 3 is a view for explaining slicing a bundled rod.

[Explanation of symbols]

 Reference numeral 10: outer cylinder 11: capillary glass tube 12: central cylinder 13: winding sheet 14: filler 30: rod 31: biopolymer array sheet

Claims (1)

[Claims] 1. A biopolymer array sheet comprising a step of attaching a biopolymer to a linear support, a step of combining a plurality of the supports, and a step of slicing a bundle of the collected supports. Production method.