JPH11106311A - Hyaluronidase activity inhibitor and its use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a hyaluronidase activity inhibitor including, as an active ingredient, a solvent extract of a specific plant and to be used by compounding in, e.g. cosmetics, drugs, quasi drugs or foods. SOLUTION: This hyaluronidase activity inhibitor comprises a solvent extract of a specific plant called, as a scientific name, e.g. Eugenia polyantha Wight (indonesian name; Babakan salam), Cinnamomum burmani Nees ex. Bl. (indonesian name; Kayu manis), Eugenia aromatica O.K. (indonesian name; Cengkeh), Quercus lusitanica Lamk (indonesian name; Majakani), Elaeocarpus ganitrus Roxb. (indonesian name; Jenitri), Elaeocarpus oxypyrena K. and V. (indoneslan name; Jenitri). Utilizing its excellent inhibitory action against hyaluronidase activity, this inhibitor is used by formulating in e.g. cosmetics, drugs, quasi drugs or foods.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hyaluronidase activity inhibitor containing a solvent extract of a specific plant and, more particularly, to a solvent extract of a specific site of a specific plant or a whole plant. The present invention relates to a hyaluronidase activity inhibitor used as a component in cosmetics, pharmaceuticals, quasi-drugs, foods and the like.

[0002]

2. Description of the Related Art Hyaluronidase is usually present in an inactive state in tissues of a living body. Hyaluronidase is activated by some kind of stimulus from inside or outside the living body, and the high molecular weight hyaluronic acid as a substrate is reduced in molecular weight. It is said that this becomes an inflammatory substance and inflammation progresses.
In addition, it has been speculated that the reduction in molecular weight of hyaluronic acid causes a decrease in skin moisturizing property, tension and the like with respect to skin. Therefore, in vitro, glycyrrhizic acid, sodium cromoglycate, baicalin, indomethacin,
Aspirin and the like have a high hyaluronidase activity inhibitory effect, and these are currently used as anti-inflammatory agents.

[0003] However, glycyrrhizic acid and baicalin have a very small amount of sweetness or a vivid vermilion color, are used as natural products since ancient times, and are said to have relatively high safety. However, there are restrictions on applications and concentrations used. In addition, sodium cromoglycate has been reported to have fetal toxicity upon administration to pregnant women and requires strict caution in its use as a pharmaceutical. Further, indomethacin and aspirin have been observed to have side effects such as rash and itchiness when used topically, and each has problems.

[0004] As described above, they have problems in safety, quality, usefulness and the like, and are limited to use in a limited range. Therefore, it has been desired to develop a hyaluronidase activity inhibitor which is safe for the human body to be applied to the skin or eats, has a remarkable inhibitory effect on the reduction of molecular weight of hyaluronic acid, and can be easily produced.

[0005]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a hyaluronidase activity inhibitor which has no side effects as seen in pharmaceutical products and has a strong inhibitory effect on hyaluronidase activity.

[0006]

DISCLOSURE OF THE INVENTION The present invention has been proposed to achieve the above object, and has been found as a result of a long-term study by the inventors of the present invention. The present invention has been completed based on a new finding that an extract extracted from a specific part of a tree or whole plant has a hyaluronidase activity inhibitory action.

That is, according to the present invention, scientific name: Eugenia polyantha Wight (Indonesia name: B
abacan salam), Scientific name: Cinnamomum burmani Nees ex Bl. (Indonesia name: Kayu manis), Scientific name: Eugenia aromatica OK (Indonesia name: Ce)
ngkeh), Scientific name: Quercus lusitanica Lamk (Indonesia name: M
ajakani), Scientific name: Elaeocarpus ganitrus Roxb. (Indonesia name:
Jenitri), Scientific name: Elaeocarpus oxypyrena K. & V. (Indonesia name: Jenitri), Scientific name: Elaeocarpus stipularis Bl. (Indonesia name:
Jenitri), Scientific name: Punica granatum L. (Indonesia name: Deli)
ma putih), Scientific name: Terminalia belerica Roxb. (Indonesia name:
(Jaha), Scientific name: Cinnamomum cassia Nees ex B1. (Indonesia name: Kembang lawang), Scientific name: Eugenia cumini Merr. (Indonesia name: Bab)
akan duwet), Scientific name: Anacardium occidentale L. (Indonesia name:
Daun jambume), Scientific name: Guazuma ulmifolia Lmk var. Tomentosa K. Schu
m. (Indonesia name: Daun jati bela
nda), Scientific name: Moschosma polystachyon (L.) Bth. (Indonesia name: Daun sangket), Scientific name: Uncaria gambir (Hunter) Roxb. (Indonesia name: Gambir), Scientific name: Adenostemma lavenia (L.) OK (Indonesia name: Legetan warak), Scientific name: Pluchea indica (L.) Less (Indonesia name: Lu)
ntas), Scientific name: Hyptis brevipes Poit. (Indonesia name: Go)
a hyaluronidase activity inhibitor comprising, as an active ingredient, at least one solvent extract of a plant selected from the group consisting of:

According to the present invention, a scientific name: Eugenia polyantha Wight (Indonesia name: B
bark, root, leaf and fruit of Abakan salam, Scientific name: Cinnamomum burmani Nees ex Bl. (Indonesia name: Kayu manis) Bark, Scientific name: Eugenia aromatica OK (Indonesia name: Ce)
ngkeh) flower buds, flowers and leaves, scientific name: Quercus lusitanica Lamk (Indonesia name: M
ajakani) insects, fruits and leaves, scientific name: Elaeocarpus ganitrus Roxb. (Indonesia name:
Jenitri) Fruit, Scientific name: Elaeocarpus oxypyrena K. & V. (Indonesia name: Jenitri) Fruit, Scientific name: Elaeocarpus stipularis Bl. (Indonesia name:
Jenitri fruit, Scientific name: Punica granatum L. (Indonesia name: Deli)
ma putih) peel, root cover, flower and fruit, Scientific name: Terminalia belerica Roxb. (Indonesia name:
(Jaha) Fruit, Scientific name: Cinnamomum cassia Nees ex B1. (Indonesia name: Kembang lawang) Fruit, root and bark, Scientific name: Eugenia cumini Merr. (Indonesia name: Bab)
akan duwet bark, leaves, nuts and seeds, scientific name: Anacardium occidentale L. (Indonesia name:
Daun jambumete) leaves, roots, bark, nuts,
Seed, scientific name: Guazuma ulmifolia Lmk var. Tomentosa K. Schu
m. (Indonesia name: Daun jati bela
nda) leaves, nuts, bark and seeds, scientific name: whole plant and leaves of Moschosma polystachyon (L.) Bth. (Indonesia name: Daun sangket), scientific name: Uncaria gambir (Hunter) Roxb. (Indonesia name: Gambir) Stem, leaf and sap, Scientific name: Adenostemma lavenia (L.) OK (Indonesia name: Legetan warak) Whole plant, root and leaf, Scientific name: Pluchea indica (L.) Less (Indonesia name: Lu)
nata) stem, leaves, roots, seeds and sap, and scientific name: Hyptis brevipes Poit. (Indonesia name: Go
and a solvent extract of at least one plant selected from the group consisting of leaves and stalks of a non-drug user as an active ingredient. The above-mentioned hyaluronidase activity inhibitor is effectively used by being blended into cosmetics, pharmaceuticals, quasi-drugs and foods.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The above-mentioned specific plant used in the present invention is commonly used as a crude drug in Indonesia, for drinking a hot water extract of a plurality of mixtures and treating abdominal pain, headache and other symptoms in Indonesia. . In the present invention, the roots, stems, xylem, leaves, fruits (including seeds), flower buds, bark,
Insects and the like are used as utilization sites, and it is important to find that this extract has a hyaluronidase activity inhibitory action.

The extraction is carried out with a solvent. As the extraction solvent, a lower alcohol, a polyhydric alcohol, a low polar solvent and a polar solvent are used. As a lower alcohol,
Methanol, ethanol, propanol and isopropyl alcohol are used. Polyhydric alcohols include glycerin and polyethylene glycol, and low-polar solvents include pentane, hexane, heptane, octane,
Saturated hydrocarbons such as nonane and decane; unsaturated hydrocarbons such as hexene and heptene; as the polar solvent, water, acetone, ethyl acetate, methyl acetate and the like are used, and these may be used alone or in combination of two or more. Used as an extraction solvent.

For the extraction, a solvent is added to Eugenia polyantha Wight or the like, and if necessary, stirring is performed at 1 to 60 ° C.
The extraction is preferably performed at room temperature or lower. However, in the case of water, it may be extracted with boiling hot water. There is no particular limitation on the amount of the mixed solvent used, but as a rough guide, dry plant (crushed material) 1
The solvent is used in an amount of 1 to 20 parts, preferably about 5 to 10 parts per part. The extract thus obtained contains a high concentration of a hyaluronidase activity inhibitory component.

As the hyaluronidase activity inhibitor according to the present invention, the extract extracted by the above method can be used as a useful component as it is, or a purified product of the extract can be used as a useful component. As the treated product, a concentrate, a paste, a dried product, and / or a diluted product of a useful component are widely used.

For example, the extract is vacuum (freeze) dried as it is, or concentrated under reduced pressure and then vacuum (freeze) dried,
Alternatively, after concentration under reduced pressure, perform solvent fractionation with various solvents,
The hyaluronidase activity inhibitory component is purified and dried (vacuum) dried, or alternatively, the extract is loaded on a column, and then the processed product is produced by a concentration purification method using column chromatography of vacuum (freeze) drying. You may. Incidentally, as the processed material, without performing the final drying step, the operation is stopped in each of the above-described steps on the way to the final drying step, and it is also possible to select and use a shape and a concentrate according to the purpose. is there.

The hyaluronidase activity inhibitor according to the present invention can be used by blending it in cosmetics, foods, quasi-drugs, pharmaceuticals and the like. In cosmetics and quasi-drugs / pharmaceutical skin preparations, various optional components (oils, humectants, thickeners, preservatives, emulsifiers, chelating agents, pigments, pH
Modifiers, medicinal ingredients, UV absorbers, fragrances) are appropriately blended and used for creams, milk lotions, lotions, foundations, packs, essences, ointments, gels, powders, lip balms, lipsticks, sun care, bath salts, etc It can be manufactured in any form according to.

Examples of the humectant include polyhydric alcohols such as glycerin, propylene glycol, 1,3-butylene glycol, sorbitol, mannitol, polyethylene glycol and dipropylene glycol; amino acids;
NMF components such as sodium lactate and sodium pyrrolidonecarboxylate, hyaluronic acid, collagen, elastin,
Water-soluble polymer substances such as chondroitin sulfate, dermatan sulfate, fibronectin, heparin-like substances, and chitosan can be exemplified.

Examples of the thickener include natural polymer substances such as sodium alginate, xanthan gum, quince seed extract, tragacanth gum, starch, and semi-synthetic polymers such as methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, soluble starch, and cationized cellulose. Examples thereof include compounds, synthetic high molecular substances such as carboxyvinyl polymers and polyvinyl polymers.

Examples of preservatives include benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, and 2,4,4'-trichloro-2'-.
Examples thereof include hydroxydiphenyl ether, 3,4,4′-trichlorocarbanide, benzalkonium chloride, hinokitiol, resorcinol, ethanol and the like.

Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, ascorbic acid and the like. Examples of the ultraviolet absorber / shielding agent include 4-methoxybenzophenone, octyldimethylparaaminobenzoate, ethylhexylparamethoxycinnamate, titanium oxide, kaolin, zinc oxide, talc and the like.

Further, as a chelating agent, for example, ethylenediaminetetraacetic acid, pyrophosphoric acid, hexametaphosphoric acid,
Examples thereof include citric acid, gluconic acid, tartaric acid, and salts thereof, and examples of the pH adjuster include sodium hydroxide, boric acid, borax, potassium hydrogen phosphate, and the like.

[0020]

[Preparation Examples] Before describing the present invention in more detail with reference to examples, examples of preparing an extract such as Euginia polyantha Wight useful as a hyaluronidase activity inhibitor used in the present invention will be described.

[Preparation Example 1] To 100 g of the crushed dried bark of Eugenia polyantha Wight was added 1,000 ml of 50 v / v% ethanol, and the mixture was stirred and extracted in a dark place at room temperature for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 2] Cinnamomum burmani Nees ex
1,000 ml of 100% ethanol was added to 100 g of Bl. Bark dried crushed product, and the mixture was stirred and extracted at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 3] Purified water of 1,000 m per 100 g of crushed dried Eugenia aromatica OK flower buds
1 was added, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Preparation Example 4] To 100 g of the crushed dried product of Quercus lusitanica Lamk insects was added 1,000 ml of 50 v / v% ethanol, and the mixture was stirred and extracted at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 5] Elaeocarpus ganitrus Roxb.
For 100 g of the crushed material of the actual dried product, 1,000 g of purified water
Then, the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 6] Elaeocarpus oxypyrena K. &
V. 100 g of purified water was added to 100 g of crushed real dried product.
0 ml was added, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 7] Elaeocarpus stipularis Bl.
For 100 g of the crushed material of the actual dried product, 1,000 g of purified water
Then, the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 8] To 100 g of a crushed dried product of Punica granatum L. peel was added 1,000 ml of purified water, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 9] Terminalia belerica Roxb.
For 100 g of the crushed material of the actual dried product, 1,000 g of purified water
Then, the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 10] Cinnamomum cassia Nees e
x B1. Purified water 1
000 ml was added, and the mixture was stirred and extracted on a hot water bath for 1 hour.
This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 11] To 100 g of crushed dried bark of Eugenia cumini Merr. Was added 1,000 ml of 100 v / v% ethanol, and the mixture was stirred and extracted at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 12] Anacardium occidentale
L. 100 v / v% for 100 g of crushed dried leaf
1,000 ml of ethanol was added, and the mixture was stirred and extracted in a dark place at room temperature for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 13] Guazuma ulmifolia Lmk va
To 100 g of the crushed r. tomentosa K. Schum. leaf dried product, 1,000 ml of purified water was added, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 14] Moschosma polystachyon
(L.) 1000 ml of purified water was added to 100 g of the crushed dried whole plant Bth., And the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 15] Uncaria gambir (Hunter)
Roxb. 1,000 ml of purified water was added to 100 g of the crushed dried stem and leaf, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 16] 100 g of crushed dried bark of Eugenia cumini Merr.
Then, the mixture was stirred and extracted in a dark place at room temperature for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 17] 100 g of a crushed dried bark of Eugenia cumini Merr.
After adding 00 ml, the mixture was extracted with stirring at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 18] 100 g of crushed dried bark of Eugenia cumini Merr.
0 ml was added, and the mixture was stirred and extracted in a dark place at room temperature for 7 days.
This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 19] Adenostemma lavenia (L.)
OK 1,000 ml of purified water was added to 100 g of the crushed dried whole plant, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 20] Pluchea indica (L.) Less
1,000 ml of 50 v / v% ethanol was added to 100 g of the crushed dried leaf, and the mixture was extracted with stirring at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract.

[Production Example 21] Hyptis brevipes Poit. Purified water 1,000 g per 100 g of crushed dried foliage
Then, the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract.

[0042]

EXAMPLE The hyaluronidase activity was tested using the extract obtained by the above production example, and it was applied to cosmetics, pharmaceuticals,
The applicability of quasi-drugs and foods was verified.

Example 1 Test of Hyaluronidase Activity Inhibiting Activity The hyaluronidase activity inhibiting effect of the extract of each Preparation Example was measured under the following conditions.

(1) Measurement method A sample was diluted to 10% with 0.1 M acetate buffer (pH 4.0), and 0.2 ml of the diluted solution was added to hyaluronidase (T
ype IV-S) solution and add 2 ml at 37 ° C.
Incubated for 0 minutes. Thereafter, 0.2 ml of a reagent was added, and the mixture was further incubated at 37 ° C. for 20 minutes.
0.5 ml of% hyaluronic acid was added and incubated at 37 ° C. for 40 minutes. The reaction is stopped by adding 0.2 ml of 0.4N sodium hydroxide solution. Thereafter, 0.2 ml of a boric acid reagent is added and the mixture is heated in a water bath for 3 minutes. p-
DAB reagent (p-dimethylaminobenzaldehyde 10
% Hydrochloric acid-acetic acid solution), and the mixture was incubated at 37 ° C. for 20 minutes, the absorbance was measured at 585 nm, and the inhibition rate (%) was obtained from the following inhibition rate calculation formula. Hyaluronidase activity inhibition rate (%) = 100-{(OD without sample-OD with sample added) / OD without sample} x 100

(2) Measurement Results The results of the inhibitory effect of the plant extract on the hyaluronidase activity are shown in Table 1. In each extract, an inhibitory effect on hyaluronidase activity corresponding to a 20% concentration of sodium cromoglycate was observed.

[0046]

[Table 1]

(3) Discussion The extract of Eugenia polyantha Wight and the like is extracted at a concentration of 10% based on the crushed dried plant. % Or less. On the other hand, sodium cromoglycate has a 0.2% concentration and the same degree of inhibition as when 10% of a plant extract is added. From these results, each extract contains about 20% or more of a hyaluronidase activity inhibitory component corresponding to sodium cromoglycate, and these extracts can be prepared with a very small amount of the hyaluronidase activity inhibitor. Can be used as raw material.

Example 2: Test of degree of skin improvement by ultraviolet irradiation method of hairless mouse From the results of Example 1, it was expected that these plant extracts would improve skin looseness and rough skin. A skin improvement test was carried out for Formulation Example 3 by a hairless mouse ultraviolet irradiation method. (1) Measuring method A hairless mouse in which loosening and rough skin were induced was previously produced by irradiating a fixed amount of ultraviolet light (UVA) daily for 4 months. These hairless mice (HR-1♀) were grouped into 5 groups (8 mice per group),
Emulsion and the like were continuously applied to the back of the hairless mouse twice a day in the morning and evening, and after three months, the degree of improvement in loosening and rough skin was examined based on the criteria shown in Table 3. Also, separately, rash in continuous application,
Side effects such as erythema were also examined.

[0049]

[Table 2]

[0050]

[Table 3]

(2) Test Results Table 4 shows the results of a test on the degree of improvement in skin looseness / roughness by ultraviolet irradiation of a hairless mouse. The numbers in the table indicate the number of animals. The results were expressed as a sum total, with “improvement degree” being 1+ and “improvement degree 2” being 2+. Extract No. 2 sample and N
o.4 sample has significant small skin looseness compared to each base.
The effect of improving rough skin was shown. No skin abnormalities were observed even after continuous use.

[0052]

[Table 4]

(3) Discussion Eugenia aromatica OK and Uncaria gambir (Hunter)
Roxb. Extract-containing cosmetics showed remarkable effects of improving skin looseness and roughness without side effects. The same improvement effect can be expected in other extracts having a hyaluronidase activity inhibitory effect.

[0054]

[Example of prescription]

[Preparation of external preparation for skin] An external preparation for skin was prepared according to the following formulation and using the extract obtained in the above Preparation Example as an active ingredient, with the composition shown below. In the formulation of the formulation example, “appropriate amount” means an amount that becomes 100% by weight in total.

<Formulation Example 1 Cream> A (% by weight) Polyethylene glycol monostearate (40.EO) 2.0 Self-emulsifying glyceryl monostearate 5.0 Stearic acid 5.0 Behenyl alcohol 1.0 Fluid Paraffin 10.0 10.0 Glyceryl trioctanoate 10.0 B Glycerin 5.0 Ethylparaben 0.1 Production Example 3 Extraction 2.0 Purified Water Appropriate Amount The components belonging to A are dissolved by heating. Separately, the components belonging to B are dissolved by heating. B was added to A, stirred, emulsified and then cooled to produce a cream.

<Formulation Example 2 Cream> A (% by weight) Polyethylene glycol monostearate (40.EO) 2.0 Self-emulsifying glyceryl monostearate 5.0 Stearic acid 5.0 Behenyl alcohol 1.0 Fluid Paraffin 10.0 Glyceryl trioctanoate 10.0 Production Example 17 Extract 1.0 B Glycerin 5.0 Ethylparaben 0.1 Purified water Appropriate amount Components belonging to A are dissolved by heating. Separately, the components belonging to B are dissolved by heating. B was added to A, stirred, emulsified and then cooled to produce a cream.

<Formulation Example 3 Emulsion> A (% by weight) Polyoxyethylene sorbitan monostearate (20.EO) 1.0 Polyoxyethylene sorbitan monostearate (60.EO) 0.5 Lipophilic glyceryl monostearate 1.0 Stearic acid 0.5 Behenyl alcohol 0.5 Avocado oil 4.0 Glyceryl trioctanoate 4.0 B 1,3-butylene glycol 5.0 Preparation example 15 extract 2.0 Ethyl paraben 0.1 Suitable amount of purified water Heat and dissolve components belonging to A. Separately, the components belonging to B are dissolved by heating. B was added to A, stirred, emulsified, and cooled to produce an emulsion.

<Prescription Example 4 Lotion> (Weight%) Ethanol 15.0 Production Example 4 Extract 1.0 Ethylparaben 0.1 Citric Acid 0.1 Sodium Citrate 0.3 1,3-butylene glycol 4. 0 Disodium edetate 0.01 Purified water Appropriate amount The above components were mixed, uniformly stirred and dissolved to produce a lotion.

Formulation Example 5 Hydrophilic ointment A (% by weight) Polyoxyethylene cetyl ether 2.0 Glycerin monostearate 10.0 Liquid paraffin 10.0 Vaseline 4.0 Cetanol 5.0 Production Example 3 Extract 1 0.0 B Propylene glycol 10.0 Methyl paraben 0.1 Purified water Appropriate amount The component belonging to A is dissolved by heating. Separately, the components belonging to B are dissolved by heating. B was added to A, stirred, emulsified, and cooled to produce a hydrophilic ointment.

[Manufacture of Cookie] Using the extract obtained in Production Example 6 as an active ingredient, a cookie was manufactured with the following composition. <Formulation Example 6 Cookie> A (% by weight) Shortening 17.4 Sucrose 24.0 Fresh cream 10.0 Egg 9.0 Production Example 6 Extract 4.0 B Light flour 34.0 Sodium hydrogencarbonate 0.6 Salts (sodium chloride) 1.0 A belonging to A are thoroughly mixed and mixed. Combine those belonging to B and sieve twice with a sieve. Mix A with B. Sprinkle the mixture on a baking sheet in a disk shape with a spoon. A baked cookie was prepared for about 20 minutes on a fire heated to 150 ° C.

[Preparation of Internal Preparation] An internal preparation having the following composition was prepared using the extract obtained in Production Example 1 as an active ingredient. <Formulation Example 7 Granules> (% by weight) Production Example 1 Extract 1.0 Lactose 30.0 Corn Starch 60.0 Crystalline Cellulose 8.0 Polyvinylpyrrolidone 1.0 Polyvinylpyrrolidone was dissolved in 50% ethanol,
This is added to lactose, crystalline cellulose and corn starch, mixed uniformly, and granulated using a granulator. After drying, the granules were sized to produce granules.

[Preparation of Tablets] Tablets were prepared with the following composition using the extract obtained in Production Example 13 as an active ingredient. <Formulation Example 8 Tablets> (wt%) citric acid 1.0 skim milk powder 15.0 sucrose ester 1.0 flaver 0.8 powdered sugar 20.0 Production Example 13 Extract 1.0 lactose 62.0 Is uniformly mixed, an appropriate amount of a 50% ethanol solution is added, and granulation is performed using a granulator. After drying, it was tableted to produce tablet confections.

[0063]

EFFECTS OF THE INVENTION The plant extract according to the present invention has an excellent inhibitory effect on hyaluronidase activity, and can be applied to all fields for preventing aging such as cosmetics, pharmaceuticals, quasi-drugs and foods. The effect can be exerted.

Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI C12N 9/99 C12N 9/99 // A21D 13/08 A21D 13/08 C09K 15/34 C09K 15/34 (72) Inventor Koichi Oshima Osaka 4-7-28 Kitahama, Chuo-ku, Yokohama City Sumitomo Forestry Co., Ltd.

Claims (6)

[Claims] [Claim 1] Scientific name: Eugenia polyantha Wight (Indonesia name: Babakan salam), Scientific name: Cinnamomum burmani Nees ex Bl. (Indonesia name: Kayu manis), Scientific name: Eugenia aromatica OK (Indonesia name: Ce)
ngkeh), Scientific name: Quercus lusitanica Lamk (Indonesia name: M
ajakani), Scientific name: Elaeocarpus ganitrus Roxb. (Indonesia name:
Jenitri), Scientific name: Elaeocarpus oxypyrena K. & V. (Indonesia name: Jenitri), Scientific name: Elaeocarpus stipularis Bl. (Indonesia name:
Jenitri), Scientific name: Punica granatum L. (Indonesia name: Deli)
ma putih), Scientific name: Terminalia belerica Roxb. (Indonesia name:
(Jaha), Scientific name: Cinnamomum cassia Nees ex B1. (Indonesia name: Kembang lawang), Scientific name: Eugenia cumini Merr. (Indonesia name: Bab)
akan duwet), Scientific name: Anacardium occidentale L. (Indonesia name:
Daun jambume), Scientific name: Guazuma ulmifolia Lmk var. Tomentosa K. Schu
m. (Indonesia name: Daun jati bela
nda), Scientific name: Moschosma polystachyon (L.) Bth. (Indonesia name: Daun sangket), Scientific name: Uncaria gambir (Hunter) Roxb. (Indonesia name: Gambir), Scientific name: Adenostemma lavenia (L.) OK Legetan warak), Scientific name: Pluchea indica (L.) Less (Indonesia name: Lu)
ntas), Scientific name: Hyptis brevipes Poit. (Indonesia name: Go)
and a solvent extract of at least one plant selected from the group consisting of: a hyaluronidase activity inhibitor as an active ingredient. 2. Bark of scientific name: Eugenia polyantha Wight (Indonesia name: Babakan salam),
Root, leaf and fruit, Scientific name: Bark of Cinnamomum burmani Nees ex Bl. (Indonesia name: Kayu manis) Scientific name: Eugenia aromatica OK (Indonesia name: Ce)
ngkeh) flower buds, flowers and leaves, scientific name: Quercus lusitanica Lamk (Indonesia name: M
ajakani) insects, fruits and leaves, scientific name: Elaeocarpus ganitrus Roxb. (Indonesia name:
Jenitri) Fruit, Scientific name: Elaeocarpus oxypyrena K. & V. (Indonesia name: Jenitri) Fruit, Scientific name: Elaeocarpus stipularis Bl. (Indonesia name:
Jenitri fruit, Scientific name: Punica granatum L. (Indonesia name: Deli)
ma putih) peel, root cover, flower and fruit, Scientific name: Terminalia belerica Roxb. (Indonesia name:
(Jaha) Fruit, Scientific name: Cinnamomum cassia Nees ex B1. (Indonesia name: Kembang lawang) Fruit, root and bark, Scientific name: Eugenia cumini Merr. (Indonesia name: Bab)
akan duwet bark, leaves, nuts and seeds, scientific name: Anacardium occidentale L. (Indonesia name:
Daun jambumete) leaves, roots, bark, nuts,
Seed, scientific name: Guazuma ulmifolia Lmk var. Tomentosa K. Schu
m. (Indonesia name: Daun jati bela
nda) leaves, nuts, bark and seeds, scientific name: whole plant and leaves of Moschosma polystachyon (L.) Bth. (Indonesia name: Daun sangket), scientific name: Uncaria gambir (Hunter) Roxb. (Indonesia name: Gambir) Stem, leaf and sap, Scientific name: Adenostemma lavenia (L.) OK (Indonesia name: Legetan warak) Whole plant, root and leaf, Scientific name: Pluchea indica (L.) Less (Indonesia name: Lu)
nata) stem, leaves, roots, seeds and sap, and scientific name: Hyptis brevipes Poit. (Indonesia name: Go
a hyaluronidase activity inhibitor comprising, as an active ingredient, a solvent extract of at least one plant selected from the group consisting of: 3. A cosmetic comprising the hyaluronidase activity inhibitor according to claim 1 or 2. 4. A pharmaceutical comprising the hyaluronidase activity inhibitor according to claim 1 or 2. 5. A quasi-drug containing the hyaluronidase activity inhibitor according to claim 1 or 2. 6. A food containing the hyaluronidase activity inhibitor according to claim 1 or 2.