JPH0240285B2 - - Google Patents

Description

[Detailed Description of the Invention] This invention relates to a method for controlling harmful bacteria in the cultivation of edible fungi mushrooms such as shiitake, nameko, oyster mushroom, and enokitake, and its purpose is to prevent any adverse effects on the edible fungi mushrooms. Instead, it aims to selectively control only harmful bacteria and prevent a decrease in the yield of the target product.

The problem of harmful bacteria is particularly important when cultivating edible mushrooms such as shiitake, nameko, and enokitake.
It is often observed that the yield of the target product is significantly reduced due to the attack of the sagebrush and fungal beds by harmful bacteria.

Hypocrea genus bacteria (Trichoderma genus) are often detected as harmful bacteria that cause these problems, and are the main cause of damage.

As a control measure against the above-mentioned Hypocrea spp. and Trichoderma spp., thiabenzimidazole (which is said to have no effect on the growth, spread, or outbreak of mushroom fungi such as shiitake, nameko, enokitake, and oyster mushrooms) is currently available. (trade name ``Panmatushiyu'') and methyl-1-2-benzimidazole carbamate (trade name ``benlate'') are known and used in some cases, but the use of these compounds has made them resistant to harmful bacteria. This has the disadvantage that the pest control effect weakens. Such bacterial resistance is observed in many plant pathogenic bacteria, and the above-mentioned harmful bacteria are no exception.

As a result of intensive research into a drug that has excellent control effects with high selectivity without being resistant to harmful bacteria in the cultivation of edible mushrooms, the inventors discovered that it is known as an antibacterial agent. 1-
[2-(2,4-dichlorophenyl)-2-(2-propenyloxy)ethyl]-1H-imidazole (trade name "imazalil") was excellent against the above-mentioned Hypocrea spp. (Trichoderma spp.) They discovered that it has a pesticidal effect and completed this invention. That is, the present invention provides cultivation equipment such as logs, saplings, or fungal beds used for cultivating edible mushrooms, or automatic inoculating machines for cultivating logs or seeds, and automatic inoculating machines for cultivating seedbeds. -[2-(2,4-dichlorophenyl)-2-(2-propenyloxy)ethyl]
This is a method for controlling pests in the cultivation of edible fungi and mushrooms, which is characterized by contacting with a liquid containing -1H-imidazole (hereinafter simply referred to as imazalil) as an active ingredient.

In this invention, as a specific means for bringing the imazalil-containing liquid into contact with the object to be treated, such as logs, oak trees, fungal beds, or the above-mentioned cultivation tools, the object to be treated is placed in an aqueous solution containing imazalil. Methods include dipping, spraying, or coating with an aqueous solution, and in some cases, it is also possible to achieve the objective by adding an aqueous solution.

In addition, it is preferable that such contact be carried out in advance before inoculating the seed of the material to be treated, but it is not necessarily limited to this, and it can also be carried out after inoculation, and generally in the case of fungal bed cultivation. It is preferable to mix it with the fungus bed medium or spray it on the fungus bed surface, and in the case of log cultivation, sprinkle or soak it on the logs or saplings.

The amount of Imazalil applied varies depending on the type of Hypocrea bacterium (Trichoderma bacterium), but as will be clear from the examples below, a sufficient control effect can be expected with a generally small amount.

The inventors used 1-[2-(2,4-dichlorophenyl)-2-(2-propenyloxy)ethyl] in shiitake mushrooms to which various Hypocrea genus bacteria (Trichoderma genus) were added. ]−1H−
Imidazole (hereinafter simply referred to as imazalil) and thiabenzimidazole (hereinafter referred to as TBZ) were added, and their concentrations and hyphal elongation rates were examined, and the results shown in FIG. 1 were obtained.

Curves 1 and 3 in Figure 1 indicate Hypocrea subainitsui as a harmful bacterium.
schweinitzii)--Also known as: Trichoderma longibranchatum
longibranchiatum)] and control with imazalil (curve 1) and TBZ (curve 3), respectively.
Curves 2 and 4 indicate the harmful fungus Hypocrea nigricans (also known as Trichoderma harthianum).
harzianum)], and shows the control by imazalil (curve 2) and TBZ (curve 4), respectively.

According to this, the effect of imazalil on mycelial elongation rate
ED ₅₀ (drug concentration showing 50% inhibition of hyphae) is 100ppm for Shiitake and 1ppm for Hypocrea schweinitzii.
(Curve 1), Hypocrea nigricans 1ppm (Curve 2)
As described above, although there are some differences depending on the type of Hypocrea bacterium (Trichoderma bacterium), imazalil used in this invention has no effect on the growth of Shiitake fungi and has a strong inhibitory effect on the growth of harmful bacteria, making it a selective choice. It can be seen that it has a medicinal effect that exceeds the above-mentioned bread matsushi and benlate which are currently used.

The experiment shown in FIG. 1 was conducted in the following manner.

That is, each drug was added to a GMY agar medium so that the final concentration was 5 to 500 PPM (weight ratio to the medium), and imazalil and TBZ were dissolved in ethanol and added to the medium to a final ethanol concentration of 1%. I made it so. The inoculum used was mycelium grown on a GMY agar medium, cut into pieces with a diameter of 6 mm using a cork borer.

Next, the edible fungal mushrooms to which the method of the present invention can be applied include the above-mentioned shiitake mushrooms, fungal mushrooms grown on logs such as nameko mushrooms, and edible fungal mushrooms grown on fungi beds such as oyster mushrooms, maitake mushrooms, kuritake mushrooms, and enokitake mushrooms. Imazalil exhibits excellent control effects against harmful bacteria without adversely affecting the growth of these bacteria.

In this regard, the inventors investigated the effects of various edible fungi on mushrooms and obtained the results shown in FIG.

That is, Figure 2 shows the elongation rate of hyphae of various edible fungi and mushrooms with respect to the concentration of Imazalil solution, where curve 1 is for Shiitake, curve 2 is for Tsukuritake, and curve 2 is for Shiitake.
Curve 3 is oyster mushroom, curve 4 is nameko, curve 5 is kuritake, curve 6 is maitake, and curve 7 is white fungus.

This shows that imazalil is particularly effective for shiitake mushrooms.

Note that this experiment was conducted using the following method.

That is, imazalil was added to a GMY agar medium so that the final concentration was a predetermined value.

For imazalil, an aqueous sulfate solution was used, and as an inoculum, mycelium grown on a GMY agar medium was cut into 6 mm pieces with a cork borer.

As described above, according to the present invention, harmful bacteria can be automatically controlled without any adverse effects on edible fungi and mushrooms, thereby preventing the desired yield drop, and moreover, it is possible to prevent harmful bacteria from decreasing. This invention is extremely useful in that it can control insects without causing fungal activity, and has great utility value.

[Brief explanation of drawings]

Fig. 1 is a graph showing the pest control effect of this invention method on shiitake mushrooms to which various Hypocrea spp. This is a graph showing the influence of various edible fungi and mushrooms.

Claims (1)

[Scope of Claims] 1. Logs, logs, mushroom beds, or cultivation tools used for cultivating edible mushrooms are 1-[2-(2,4-dichlorophenyl)-2-(2-propenyloxy)] A method for controlling harmful bacteria in the cultivation of edible fungi and mushrooms, which comprises contacting with a liquid containing ethyl]-1H-imidazole as an active ingredient.