JPH09309812A - Antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidant developing significant antioxidant effect as compared with those of conventional ones, and containing new compound (s) useful for stably formulating foods or cosmetics with substance (s) low in oxidation stability and having been hard to formulate. SOLUTION: This antioxidant contains, as active ingredient, 0.001-20.0wt.% of saikosaponin b1 , saikosaponin b2 , or an extract containing either saikosaponin b1 or saikosaponin b2 . The saikosaponin b1 or saikosaponin b2 is obtained by subjecting the leaves and stems or roots, etc., of bupleuri radix, or bupleuri radix whole grass to extraction with e.g. water as extractive solvent (i.e., through the following consecutive processes: cold immersion or refluxing under heating, filtration, and concentration). Formulation of a milky lotion with this antioxidant enables stable formulation of the milky lotion with e.g. turtle oil.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel antioxidant having an excellent antioxidant action.

[0002]

BACKGROUND OF THE INVENTION Hitherto, dibutylhydroxytoluene (BHT), butylhydroxyanisole (BHA), tocopherol and the like have been used as antioxidants for foods and cosmetics containing oils and fats. However, synthetic antioxidants such as BHT and BHA have safety problems, and the amount added is limited. Therefore, natural antioxidants have been desired. The present invention has been made in view of such conventional circumstances, has a high effect as an antioxidant, has no toxicity problem, and can be used as food, cosmetics, pharmaceutical raw materials and the like. It is intended to provide an antioxidant having a high content.

[0003]

[Means for Solving the Problems] To achieve the above object, the inventors of the present invention have conducted extensive studies on the antioxidant activity of natural products, and as a result, have found that saikosaponin b ₁ , saikosaponin b ₂ or a saiko extract containing either of them. Have been found to be extremely effective as antioxidants.

[0004] That is, the present invention is characterized in that the active ingredient is psychosaponin b ₁ , psychosaponin b ₂ , or a psychoextract containing either psychosaponin b ₁ or psychosaponin b ₂ as an active ingredient. Is.

Hereinafter, the configuration of the present invention will be described in detail. Saikosaponins b ₁ used in the present invention, saikosaponin b _2, Psycho extract containing either saikosaponins b ₁ or saikosaponins b ₂ are both rough skin improving effect than the conventional, that a anti-aging effect known (JP-A-61-2216, JP-A-4-29916)
Japanese Patent Publication No.), a skin external preparation.
However, it is not yet known that these components have an antioxidant action, and they have been found for the first time by the present inventors.

The saikosaponin b ₁ and saikosaponin b ₂ of the present invention are subjected to cold soaking or heating under reflux of whole saiko such as saiko leaves and stems or roots with an extraction solvent, followed by filtration,
Obtained by concentrating. The extraction solvent used in the present invention may be any solvent that is usually used for extraction, particularly water,
Alternatively, alcohols such as 1,3-butanediol, ethanol and methanol, hydrous alcohols, acetone,
Organic solvents such as ethyl acetate can be used alone or in combination. Compounding amount is 0.001-2
0.0 wt% is suitable. If it is less than 0.001% by weight, the antioxidant power is insufficient, and if it exceeds 20.0% by weight, it is unnecessary in terms of antioxidant power. It is not practical because it gets worse.

Cosmetics are preferable as an object to be blended as an antioxidant, and when added to various cosmetics requiring antioxidant action, such as creams, lotions, milky lotions, hair oils, shampoos, rinses and soaps, they have excellent antioxidant power. Exert. Other additives to be added to such cosmetics include, for example, oils, surfactants, thickeners, neutralizers, preservatives, powder components, pigments, sequestering agents, fragrances, and ultraviolet absorption. Agents, medicinal agents, etc., and are used in appropriate combinations as needed. As a matter of course, the antioxidant of the present invention can be used in combination with other antioxidants such as vitamin E, licorice extract, BHT, and BHA as long as the effects of the present invention are not impaired. In particular, by using the antioxidant of the present invention, even a substance which has been conventionally difficult to be compounded due to poor oxidation stability can be stably compounded in the system. For example, turtle oil, mink oil, and the like, which are expected to have an emollient effect when applied to cosmetics, have poor oxidative stability, and their use is limited. By adding, the fats and oils can be prevented from being oxidized, and these fats and oils can be effectively incorporated into cosmetics. Recently, application of an antioxidant has been proposed for the purpose of preventing lipid peroxidation reaction in a biological system, but the antioxidant of the present invention can of course be used in a biological system.

[0008]

It is confirmed by the present inventors that the antioxidant activity of the saikosaponin b ₁ , the saikosaponin b ₂ , and the saiko extract containing either saikosaponin b ₁ or saikosaponin b ₂ is strong. Was done.

(1) 5,5-dimethyl-1-pyrroline oxide (D) was added to a mixed solution of 50 μl of 1 mM ferrous sulfate (FeSO ₄ ) solution and 75 μl of the sample (aqueous solution) shown in Table 1.
20 μl of 10% aqueous solution of MPO) was added, followed by 1 mM
75 μl of hydrogen peroxide (H ₂ O ₂ ) was added and stirred. H ₂ O ₂
The radical adduct of hydroxy radicals (.OH) (DMPO-OH) generated 75 seconds after the addition of was added was measured using an electron spin resonance apparatus (ESR). The hydroxyl radical scavenging activity was determined on the assumption that the hydroxyl radical scavenging activity of distilled water was 0%. Table 1 shows the results. In the table, valerian and Gymnema are both known as natural antioxidants. Further, the psycho extract contains 0.05% by weight of saikosaponin b ₁ and 0.05% by weight of saikosaponin b ₂ .

[0010]

[Table 1] ────────────────────────────── Hydroxy radical scavenging activity ───────────── ────────────────── Control (water) 0% Valerianum (10mg / ml) 8% Gymnema (10mg / ml) 23% Saikosaponin b ₁ (10mg / ml) 58 % Saikosaponin b ₂ (10 mg / ml) 57% Saiko extract (10 mg / ml) 45% ────────────────────────────── ──

(2) To 5 μl of the sample (aqueous solution) shown in Table 2, 50 μl of the coloring reagent and 50 μl of the enzyme solution were added, and after standing at 37 ° C. for 20 minutes, 100 μl of the reaction stop solution was added,
The absorbance at 570 nm was measured. However, the coloring reagent is a mixture of 0.1 M phosphate buffer pH 8, 0.4 mM xanthine, and 0.24 mM nitro blue tetrazolium. The enzyme solution is 0.049 U / ml xanthine oxidase. The reaction stop solution is 69 mM sodium dodecyl sulfate. The superoxide erasing activity was determined by converting it to the erasing rate by standard SOD. The results are shown in Table 2. The psycho extract contains 0.05% by weight of saikosaponin b ₁ and 0.05% by weight of saikosaponin b _2.
It contains.

[0012]

[Table 2] ───────────────────────────────── Superoxide scavenging activity ───────── ──────────────────────── Control (water) 0 U / ml Valerianum (10 mg / ml) 0 U / ml Gymnema (10 mg / ml) 0 U / ml Psychosaponin b ₁ (10 mg / ml) 15 U / ml Psychosaponin b ₂ (10 mg / ml) 14 U / ml Psycho extract (10 mg / ml) 7 U / ml ─────────── ───────────────────────

[0013]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Example 1 Emulsion Stearic acid 2.5% by weight Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 7.0 Turtle oil 3.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3.0 Triethanolamine 1.0 Psycho 1,3-butylene glycol extract 0.02 (Psychosaponin b ₁ = 0.03% by weight, Psychosaponin b ₂ = 0.03% by weight) Fragrance Suitable amount Preservative Suitable amount Purified water Residual (manufacturing method) Polyoxyethylene glycol 1500 and triethanolamine were added to ion-exchanged water, and the mixture was heated and dissolved and kept at 70 ° C (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). Add oil phase to water phase, emulsify uniformly with homomixer, and after emulsification, stir well
C. to give an emulsion.

Example 2 Cream Beeswax 10.0 wt% Cetyl Alcohol 5.0 Hydrogenated Lanolin 5.0 Squalane 37.5 Mink Oil 3.0 Glyceryl Monostearate 2.0 Polyoxyethylene (20 mol) Sorbitan Monolaurin Acid ester 2.0 Propylene glycol 5.0 Psychosaponin b ₁ 0.02 Fragrance Suitable amount Preservative Suitable amount Purified water Residual (production method) Add propylene glycol to ion-exchanged water,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). The oil phase was added to the water phase to carry out preliminary emulsification, and after uniform emulsification with a homomixer, the mixture was cooled to 30 ° C. while stirring well to obtain a cream.

Example 3 Cream cetostearyl alcohol 3.5% by weight squalane 40.0 beeswax 3.0 reduced lanolin 5.0 ethylparaben 0.3 polyoxyethylene (20 mol) sorbitan monopalmitate 2.0 stearic acid Monoglycoside 2.0 Psychosaponin b ₂ 5.0 Perfume 0.03 1,3-Butylene glycol 5.0 Glycerin 5.0 Sodium hyaluronate 0.05 Purified water Residual (production method) cetostearyl alcohol, squalane, beeswax, reduction Lanolin, ethylparaben, polyoxyethylene (20 mol) sorbitan monopalmitate, stearic acid monoglycoside, saikosaponin b ₂
And melt the fragrance with heating and keep it at 75 ℃,
Add to 1,3-butylene glycol, glycerin, sodium hyaluronate and purified water warmed to 0 ° C with stirring. The mixture was treated with a homomixer to make the emulsified particles finer, and then rapidly cooled with stirring to obtain a cream.

Example 4 Emulsion Acetone extract of saiko 1.0% by weight (Saikosaponin b ₁ = 0.02% by weight, saikosaponin b ₂ = 0.02% by weight) Stearic acid 1.5 Cetyl alcohol 0.5 Beeswax 2.0 Polyoxyethylene (10 moles) Monooleate 1.0 Stearic acid monoglycoside 1.0 Quinceseed extract (5% aqueous solution) 20.0 Propylene glycol 5.0 Ethanol 3.0 Ethylparaben 0. 3 Methylparaben 0.05 Perfume 0.05 Purified water Residue (production method) Psychoacetone extract and perfume are added to ethanol and dissolved (alcohol solution). Propylene glycol is added to purified water, and the mixture is dissolved by heating and maintained at 70 ° C. (aqueous phase). Mix other ingredients except quince seed extract,
Melt by heating and keep at 70 ° C (oil phase). The oil phase is added to the water phase, pre-emulsified, and homogenized uniformly with a homomixer. While stirring this, an alcohol solution and a quince seed extract are added. Then, it was rapidly cooled to 30 ° C. with stirring to obtain an emulsion.

[0018]

As described above, according to the present invention,
It can exhibit a remarkable antioxidant effect as compared with conventional antioxidants. Further, by using the antioxidant of the present invention,
Even substances that are poor in oxidative stability and difficult to be compounded can be stably compounded in the system, and are expected to be used in a wide range of products such as cosmetics and foods.

Claims (3)

[Claims] 1. An antioxidant, which comprises as an active ingredient a saikosaponin b ₁ , a saikosaponin b ₂ , or a saiko extract containing either saikosaponin b ₁ or saikosaponin b ₂ . 2. The compounding amount of Psychosaponin b ₁ , Psychosaponin b ₂ , or Psycho extract containing Psychosaponin b ₁ or Psychosaponin b ₂ is 0.00.
The antioxidant according to claim 1, which is 1 to 20.0% by weight. 3. A base material for cosmetics, which comprises psychosaponin b ₁ and / or psychosaponin b ₂ as an antioxidant.