JPH09127084A - Liquid chromatograph detector - Google Patents

Abstract

(57) 【Abstract】 PROBLEM TO BE SOLVED: It is possible to obtain a stable temperature in a short time as compared with a conventional liquid chromatograph detector, and to obtain highly reliable data which is not affected by fluctuations in outside air temperature. A detector for a liquid chromatograph is provided. SOLUTION: A cell chamber 8 of the detector is provided with a temperature detector 12 and a heater 14, and a temperature setting device 10 for setting the temperature of the cell chamber 8, a temperature detector 13 for detecting the outside air temperature, and temperature detectors 12, 13 are provided. A control device 15 for controlling the rotation speed of the fan 11 and the strength of the heater 14 is provided. When the temperature of the cell chamber 8 immediately after the power is turned on is lower than the stable temperature (arbitrary temperature) or when the outside air temperature fluctuates after the temperature is stabilized, the rotation speed of the fan 11 and the strength of the heater 14 are controlled according to the temperature change, The temperature of the cell chamber 8 is stabilized in a short time,
Keep the temperature of (constant temperature) constant.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid chromatograph detector, and more particularly to a liquid chromatograph detector capable of controlling the strength of the heater and the rotation speed of a fan.

[0002]

2. Description of the Related Art The construction of a liquid chromatograph will be described with reference to FIG. FIG. 2 is a block diagram of a liquid chromatograph.
The configuration includes a pump 21, an injector (or autosampler) 22, a column 23, a detector 24, and a data processing device 25. The eluent is sent by the pump 21 at a high pressure constant flow rate. Injector (or auto sampler) 2
The sample injected by 2 is sent to the column 23 and separated into each component due to the difference in affinity with the packing material in the column 23. Each component eluted from the column 23 is converted into a change in electric signal by the detector 24, and is identified and quantified by the data processing device 25. In the conventional liquid chromatograph detector, there is no heater for adjusting the temperature of the cell chamber, and the rotation speed of the fan is constant.

[0003]

Since the heat of the light source is released by the fan after the power is turned on, the rise time until the temperature of the cell chamber reaches a stable temperature is delayed. Further, since the rotation speed of the fan is also constant, the temperature of the cell chamber is affected by the fluctuation of the outside air temperature, and the baseline is fluctuated.

[0004]

In order to achieve the above object, the detector of the liquid chromatograph of the present invention is provided with a heater for warming the cell chamber in the conventional detector, and the temperature of the cell chamber is stable. The rise time is shortened until it reaches (arbitrary temperature). Further, a temperature detector for detecting the temperature of the cell chamber and the outside air is provided, and a control device for controlling the rotation speed of the fan and the strength of the heater by the temperature detector is provided, and the cell The temperature of the room is constant.

In the present invention, a heater and a temperature detector are provided in the cell chamber, and a temperature setting device for setting a stable temperature (arbitrary temperature) of the cell chamber is provided. ) Is set and the temperature of the cell chamber is lower than the stable temperature (arbitrary temperature), the heater is activated and the fan is stopped. When the temperature approaches the stable temperature, the heater is stopped and the fan is activated to reduce the temperature of the cell chamber. A stable temperature (arbitrary temperature) can be achieved over time. Further, even after the temperature is stabilized, if the outside air temperature changes, the temperature of the cell chamber is also affected, and the temperature detector detects the temperature change of the cell chamber. The temperature of the cell chamber can be kept constant (stable temperature) by the control device controlling the rotation speed of the fan and the strength of the heater by the output of the temperature detector.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION An embodiment of a liquid chromatograph detector of the present invention will be described with reference to FIG. FIG. 1 is a block diagram of the detector of the present invention. The detector is a spectroscope including the light source 1, the slits 2, 5, the diffraction grating 3, the reflecting mirrors 4, 6, the cell chamber 7, the cell chamber 8 and the photodetector 9, and the temperature for setting the temperature of the cell chamber 8. Setting device 10, fan 11 for cooling the inside of the detector, temperature detector 1 for detecting the temperature of the cell chamber
2. A temperature detector 12 for detecting the outside air temperature, a heater 14 for warming the cell chamber 8 and the like. After the power is turned on, the stable temperature (arbitrary temperature) of the cell chamber 8 is set in the temperature setting device 10.
Generally, the temperature of the cell chamber 8 immediately after the power is turned on is almost equal to the outside air temperature, and is gradually warmed by conduction, radiation, etc. by the heat generation of the light source 1. When the temperature of the cell chamber 8 is lower than the stable temperature (arbitrary temperature), the temperature detector 12 detects this and the controller 15 stops the fan 11 and operates the heater 14 to stabilize the temperature of the cell chamber 8. Fan 1 approaches the temperature (arbitrary temperature)
By operating 1 and stopping the heater 14, the time to a stable temperature (arbitrary temperature) can be shortened as compared with a conventional detector. After that, the temperature change where the outside air temperature changes is transmitted to the cell chamber 8, the temperature detector 12 detects this temperature change, and the controller 15 controls the fan 11 and the heater 14 to cause the cell chamber 8 to change. The temperature can be kept constant (stable temperature).

FIG. 3 shows a comparison of the time from when the power is turned on to when the temperature of the cell chamber 8 stabilizes, between the detector of the present invention and the conventional detector. As shown in FIG. 3, the conventional product has a fan 1.
The rotation speed of 0 is constant, and it takes about 90 minutes until the temperature of the cell chamber 8 reaches a stable temperature (about 30 ° C.). On the other hand, in the product of the present invention, if an arbitrary stable temperature (eg, 30 ° C.) is set after the power is turned on, the fan 11 is stopped, the heater 14 has an arbitrary strength, and the temperature of the cell chamber 8 is a stable temperature (set temperature). 30
(° C), the heater 14 is stopped, the fan 11 operates at high speed to prevent overheating, and the medium temperature is gradually increased to reach a stable temperature (arbitrary temperature; 30 ° C) in about 45 minutes. It can be done and the time can be shortened significantly.

FIG. 4 shows the number of revolutions of the fan 11 of the conventional product, the temperature of the cell chamber 8 and the drift data at that time with respect to the fluctuation of the outside air temperature. As shown in FIG. 4, in the conventional product, the rotation speed of the fan 11 is constant with respect to the change in the outside air temperature, so that the temperature of the cell chamber 8 is also affected, which greatly affects the drift data.

FIG. 5 shows the rotation speed of the fan 11 of the present invention, the strength of the heater 14, the temperature of the cell chamber 8 and the drift data at that time with respect to the fluctuation of the outside air temperature. As shown in FIG. 5, in the product of the present invention, when the outside air temperature rises, the temperature change is transmitted to the cell chamber 8 and is detected by the temperature detector 12, and the control device 15 controls the rotation speed of the fan 11 according to the temperature change. Raise,
Prevent the temperature rise of the cell chamber 8. Further, when the outside air temperature decreases, the temperature detector 12 in the cell chamber 8 detects the temperature, and the control device 15 reduces the rotation speed of the fan 11 according to the change in temperature, so that the heater 1
4 also operates gradually. When the outside air temperature rises again, the heater 14 is stopped, and the temperature of the cell chamber 8 can be kept constant by increasing the rotation speed of the fan 11 according to the temperature change. Therefore, the product of the present invention can obtain highly reliable data that is not affected by changes in the outside air temperature.

[0010]

According to the present invention, the temperature of the cell chamber can be made stable (arbitrary temperature) in a short time as compared with the conventional detector. Further, it is possible to keep the temperature of the cell chamber constant even if the outside air temperature fluctuates. Therefore, the drift caused by the temperature change of the cell chamber can be reduced, and highly reliable data can be provided.

[Brief description of the drawings]

FIG. 1 is a block diagram of a liquid croctograph detector of the present invention.

FIG. 2 is an explanatory diagram of a liquid chromatograph.

FIG. 3 is a relationship diagram of time and temperature from power-on to stable temperature in a conventional product and a product of the present invention, and a relationship diagram of fan rotation speed and heater strength at that time.

FIG. 4 is a relationship diagram of a fan rotation speed, a temperature of a cell chamber, and drift data at that time with respect to a change in outside air temperature in a conventional product.

FIG. 5 is a relational diagram of fan rotation speed, heater strength, cell chamber temperature, and drift data at that time with respect to changes in the outside air temperature in the product of the present invention.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Light source (D ₂ lamp), 2, 5 ... Slit, 3 ... Diffraction grating, 4, 6 ... Reflecting mirror, 7 ... Cell chamber part, 8 ... Cell chamber, 9
... Photodetector, 10 ... Temperature setting device, 11 ... Fan, 1
2, 13 ... Temperature detector, 14 ... Heater, 15 ... Control device.

Claims (3)

[Claims] 1. A liquid chromatograph irradiates a liquid flowing out of a column and passing through a flow cell of a cell chamber with light,
A detector for detecting light absorption or fluorescence by the liquid, comprising a heater for heating the liquid passing through the flow cell, the detector of a liquid chromatograph. 2. The fan according to claim 1, further comprising: a fan for cooling the light source, the heater being operated, and the fan being arbitrarily controlled and operated after a predetermined time. Liquid chromatograph detector. 3. A liquid chromatograph detector comprising the heater according to claim 1 and the fan according to claim 2, wherein a temperature setting device for setting the temperature of the cell chamber,
A temperature detector for detecting the temperature of the cell chamber and the outside air is provided,
The fan is a liquid chromatograph detector controlled based on the set temperature of the temperature setting device and the output of the temperature detector.