JPH08242814A - Food and cake composition - Google Patents

Abstract

PURPOSE: To obtain a food and cake composition by preparing an active ingredient showing excellent antimicrobial activity against organisms causing dental caries and periodontal diseases and having extremely high safety to human body as a food and a cake, capable of successively being taken even in the case of an infant daily with ease so as to prevent dental caries and periodontal diseases. CONSTITUTION: The food and cake composition comprises an extract obtained from a plant of the genus Murraya as an active ingredient. Murraya koenigii Spreng or Murraya paniculata Jack may be cited as the plant of the genus Murraya. A compound such as isomahanine, mahanine, murrayanol, etc., is contained in the active ingredient.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food and confectionery composition, the purpose of which is extremely high in safety for the human body, and even infants can safely eat it. The present invention provides a food and confectionery composition that can easily prevent (cavities) and periodontal disease.

[0002]

BACKGROUND OF THE INVENTION Caries (dental caries) and periodontal disease are considered to be two major diseases in the field of dentistry, and the prevalence of dental caries is particularly high in the Japanese population. It is said that dextran produced by the action of sugars in foods and Streptococcus mutans that are resident in the oral cavity are largely involved in the occurrence of tooth decay. Dextran is Streptococcus mutans
Attached to the cell surface of. Streptococcus mutans ( Streptoco) is added to this dextran.
ccus mutans ) bacteria, food waste, various fungi and the like adhere in layers to form plaque on the tooth surface. Lactic acid is produced by bacteria in dental plaque, but as the layer of dental plaque becomes thicker, the lactic acid produced in the deep layer of dental plaque becomes less likely to be buffered by saliva, and as a result, the tooth surface is gradually dissolved. That causes tooth decay. On the other hand, periodontal disease is a general term for diseases in base tissues that support teeth, that is, periodontal ligament, gingiva, and alveolar bone, and is said to be caused by impurities in the oral cavity such as plaque and tartar. That is, periodontal disease occurs due to inflammation of the mucous membrane in the gingival sulcus due to bacteria in the plaque or tartar and its producing enzyme, or ulcer due to infection with Pseudomonas aeruginosa. Bacteria involved in periodontal disease include Polyphyromonas gingivalis ( Porphyromonas gin)
givalis ) is known as a typical one.

[0003]

2. Description of the Related Art It is said that it is most effective to brush teeth within 30 minutes after eating to prevent tooth decay and periodontal disease. However, it is almost impossible to carry a toothbrushing tool all the time, and it is often difficult to secure a place and time for brushing teeth when going out. Therefore, Streptococcus mutans ( Stre , which causes tooth decay and periodontal disease )
ptococcus mutans ), Polyphyromonas gingivalis ( Porphyromonas gi)
A method for preventing these diseases by administering an oral composition containing a substance that suppresses the growth or inhibits the growth of oral bacteria such as N. gingivalis ) is also adopted.

[0004] Specifically, chlorhexidine, cetylpyridinium chloride, etc. are known as oral bactericides, but there are some concerns about safety such as bacterial flora and effects on human body when administered to infants. . Since the morbidity rate of dental caries is high in infants, and it is very important to prevent dental caries continuously on a daily basis, there is a need for highly safe dental caries. Various studies have been conducted on the use of a plant extract as an active ingredient in oral products. For example, as an antibacterial agent against caries and periodontal disease-causing bacteria, fennel extract is disclosed in JP-A-61-260.
No. 012 publication.

[0005]

However, there has been a problem that none of the above-mentioned extracts has satisfactory antibacterial activity against the causative bacteria of caries and periodontal disease. Therefore, the present invention exhibits excellent antibacterial activity against bacteria causing caries and periodontal disease, does not affect the flora and the human body, and can be safely orally ingested even in infants. A highly safe active ingredient that can be provided in a form that can be easily ingested daily such as food and confectionery, and an object of the invention is to provide an excellent food and confectionery composition that can prevent tooth decay and periodontal disease in a normal diet. To do.

[0006]

The present invention has been made to solve the above problems, and in the food and confectionery composition according to claim 1, an extract obtained from a plant of the genus Muraya is an essential component. And

[0007]

[Function] An extract obtained from a plant of the genus Muraya is used for Streptococcus mutans or polyphyllomonas gingivalis ( Porphyromon ), which are the causes of tooth decay and periodontal disease.
as gingivalis ) and has extremely high antibacterial activity against oral bacteria. Therefore, since foods and confectionery compositions containing such an extract are extremely safe for the human body, they can be provided with peace of mind to people of all ages, from infants to the elderly, and are routinely used as foods and confectionery. While continuously ingesting, it is possible to easily prevent tooth decay and periodontal disease.

[0008]

The constitution of the food and confectionery composition according to the present invention will be described in detail below. In the food and confectionery composition of the present invention, an extract obtained from a plant of the genus Muraya is an essential component. A plant of the genus Muraya is a kind of Rutaceae plant, and specifically, Murraya koenigi
i Spreng) or Geckets ( Murrayap )
aniculata Jack) is preferably used. Geckitsu ( Murraya paniculata)
Jack) is a plant that grows in relatively dry open forests and is used in the purification of aromatic oils, which is distributed in Taiwan, Fuzhou, Guangdong, Guangxi, and tropical and subtropical regions of Asia. The whole plant is used as a medicinal substance and the leaves are used as a spice, which is an extremely safe plant for the human body.

In the present invention, the above-mentioned geckets ( Mu
rraya paniculata Jack or Murraya koenigii Sp
It can be used in any part of the whole plant or leaves, stems, rhizomes, fruits, etc. of Muraya plants such as reng), and the extract from the whole plant or a part or more thereof is an essential component. Alternatively, more preferably, Murraya pan
iculata Jack) or lizard ( Mur
It is preferable to use leaves of a plant of the genus Muraya such as Raya koenigii Spreng) as a starting material because the active ingredient can be efficiently extracted. Such extraction raw material in a dry state,
Alternatively, in a non-dried state, it is shredded if necessary and extracted with a solvent.

The solvent used for the extraction may be a polar solvent or a non-polar solvent and is preferably used without limitation. As the polar solvent, water, an acid or alkaline aqueous solution, or an aqueous solution of a salt thereof, a lower alcohol having 1 to 5 carbon atoms, an ester of a lower alcohol having 1 to 3 carbon atoms and a fatty acid, or a ketone having 1 to 3 carbon atoms Alternatively, these hydrates are preferably used, specifically methanol,
Examples include ethanol, propanol, or water-containing products thereof, ethyl acetate, acetone, propylene glycol and the like. As the non-polar solvent, hydrocarbons having 4 to 12 carbon atoms, petroleum ether, ethers having 3 to 6 carbon atoms,
Any halogen-containing compound having 1 to 4 carbon atoms may be used, and specifically, n-hexane, cyclohexane, petroleum ether, ligroin, ethyl ether, diethyl ether,
Examples include dioxane, dichloromethane, 1,2-dichloroethane, carbon tetrachloride, butadiene, chloroform, toluene, benzene and the like. Such polar solvent, one kind of a non-polar solvent or a mixture of any two or more kinds thereof can be used as an extraction solvent without particular limitation in the present invention, but it is more preferable to use a polar solvent as the extraction efficiency. It is good and preferable.

The extraction method is not particularly limited,
It can be carried out according to a conventional method. The starting material is immersed in the extraction solvent at room temperature or under heating, and then the solvent is distilled off from the extraction liquid to obtain an extract. 1 to 12 when immersed at room temperature
For about 0 hours, when the extraction with heating is performed, the solvent is 40 to 7
It is preferable to perform the heating at 0 ° C. After the extraction, the extract is concentrated under reduced pressure, the solvent is distilled off, and then the extract is washed. Water, warm water, or steam can be used for cleaning. When washing with water or warm water, 0.1 to 50 times the weight of the extract, more specifically 5 to 30 times the weight of water or warm water may be used. Washing is performed in the range where the amount of liquid discharged is 0.1 to 50 times the weight. This washing is preferably performed several times. By performing the washing as described above, the water-soluble coloring component and the bitterness component can be removed.

Further, in the present invention, the extraction residue after obtaining the predetermined extract may be subjected to secondary extraction using a solvent. In this case, for the secondary extraction from the extraction residue,
The same solvent used in the first extraction can be used, but a different solvent may be used. When a non-toxic solvent such as water, ethanol, or a water-ethanol mixed solution is used as the extraction solvent, the extraction liquid can be used as it is as the active ingredient of the present invention.

In addition to the above-mentioned method, for example, the starting material is first subjected to primary extraction with a polar solvent, the solvent is distilled off from the obtained extract, followed by washing, and the washed product is subjected to secondary extraction with a polar solvent again. It is also possible to employ a method in which the desired extract is obtained by performing a selective extraction and distilling off the solvent. Furthermore, the starting material is subjected to primary extraction with a polar solvent, the solvent is distilled off from the obtained extract, and then the washed product is washed, and then the washed product is dissolved in a non-polar solvent and washed to obtain the desired extraction. It is also possible to adopt a method of obtaining a product. Alternatively, it is possible to employ a method in which the starting material is dissolved in a non-polar solvent in advance to remove contaminants, and then extraction with a polar solvent is performed.

Further, according to the present invention, the essential oil can be removed by steam distillation for the purpose of deodorizing before or after obtaining the extract from the starting material. More specifically, for example, when steam distillation is used, the amount of distillate is preferably 1 to 20 times the weight of the raw material plant, more preferably 2 to 10 times the weight. The reason for this is that if the amount of distillate is less than the same amount as that of the raw material plant, sufficient essential oil cannot be extracted, and on the other hand, even if it is used in excess of 20 times the weight, the amount of extracted essential oil cannot be expected to increase. This is because neither case is preferable. Alternatively, instead of steam distillation, a treatment with an adsorbent may be performed as a pretreatment or a posttreatment of the extraction step, and there is no particular limitation. As the adsorbent, any of activated carbon, activated alumina, diatomaceous earth, kaolin, calcium silicate, activated clay, silica gel and the like can be used without any particular limitation.

The active ingredient is recovered from the plant of the genus Muraya by the above steps. This active ingredient contains isomahanine represented by the following formula 1 (formula 1), murayanol represented by the following formula 2 (formula 2), and mahanine represented by the following formula 3 (formula 3). In the present invention, one or more of these compounds may be used as the active ingredient. Further, such a compound is preferably contained in the extract in an amount of about 1 to 50% in order to sufficiently exhibit the effect as an antibacterial agent.

[0016]

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Foods and confectionery compositions are prepared by using the above-mentioned active ingredients as essential ingredients. In the food and confectionery composition of the present invention, it is desirable that the above-mentioned active ingredient is blended in a total amount of 0.0001 to 5% by weight, more preferably 0.001 to 3% by weight. The reason for this is that the extract of Muraya plants or the compounding amount of at least one compound of isomahanin, mahanin, and murayanol is 0.0 in the food and confectionery composition.
If the amount is less than 001 parts by weight, Streptococcus mutans ( Streptoc) is considered to cause tooth decay and periodontal disease.
occus mutans ), Porphyromonas gingivali ( Porphyromonas gingiv)
This is because the antibacterial activity against oral bacteria such as alis ) is not sufficient, and even if it exceeds 5% by weight, the activity proportional to the compounding amount cannot be obtained, which is not preferable in any case.

The form of the food and confectionery composition is not particularly limited, and caramel, juice, yogurt, gum, ice cream, cookie, pudding, mousse, jelly, instant powder of confectionery such as pancakes, stew, etc. It can be appropriately prepared as ordinary foods and confectionery such as processed foods such as curry and curry, baby foods and the like.

[0019]

EXAMPLES Hereinafter, the food and confectionery composition according to the present invention will be described more specifically by showing examples. However, the present invention is not limited to the following embodiments.

(Embodiment 1) Black fox ( Murra )
1 kg of leaves ( ya koenigii Spreng) were extracted with 20 kg of ethyl acetate at room temperature for one day, and the obtained extract was concentrated under reduced pressure to obtain an extract. 8 for this extract
500 g of warm water at 0 ° C. was added and stirred, and the mixture was allowed to stand until it was separated into a water layer and a resinous solid content layer. The aqueous layer was separated and removed by decantation, and this step was repeated 3 times to obtain a sample of Example 1. (Example 2) Murakyan panic
1 kg of the fruit ( lata Jack) was extracted with 20 kg of methanol at room temperature for one day, and the obtained extract was concentrated under reduced pressure to obtain an extract. Warm water of 80 ° C to this extract 500
g was added and stirred, and the mixture was allowed to stand until it was separated into a water layer and a resinous solid content layer. The aqueous layer was separated and removed by decantation, and this step was repeated 3 times to obtain a sample of Example 2. (Example 3) A mahanine compound was used as a sample of Example 3. (Example 4) A murayanol compound was used as a sample of Example 4.

Comparative Example 1 Commercially available fennel oleoresin was used as a sample of Comparative Example 1.

[0022]

[Test Example] Regarding the samples of the above Examples and Comparative Examples,
Streptococcus mutans (Strepto
coccus mutans ), polyphyllomonas
Gingivalis ( porphyromonas ging)
ivalis ) was determined. First, as a liquid medium, for Streptococcus mutans , a THB 3% by weight-added medium was added to polyphyllomonas.
Gingivalis ( porphyromonas ging)
ivalis ) THB 3% by weight, hemin 3p
Mediums containing pm and menadione at 0.5 ppm were prepared. Into a test tube containing 4 ml of this liquid medium, 0.04 ml of each of the samples of Examples 1 to 4 and Comparative Example 1 whose concentrations were changed stepwise was added and stirred. 0.04 ml of each of the two types of precultured bacterial solutions was added thereto and stirred. After anaerobically culturing this test tube at 37 ° C. for 3 days, the absorbance at 550 nm of this culture solution was measured to measure the growth amount of the bacterial cells. The absorbance value of less than 0.05 was validated, and the minimum inhibitory concentration of the above-mentioned two types of bacterial cells was determined. Table 1 shows the results.

[0023]

[Table 1]

As is clear from the results shown in Table 1, in the samples of the examples, Streptococcus mutans ( Streptococcus m) was compared with the samples of the comparative examples.
utans ) and Polyphilomonas gingivalis ( Po
It can be seen that it exhibits extremely high antibacterial activity against bacteria causing tooth decay and periodontal disease such as rphyromonas gingivalis ).

[0025]

[Formulation Examples] Formulation examples of the food and confectionery composition of the present invention will be exemplified below. The present invention is not limited to the following prescription.

(Formulation Example 1) Candy (g) Sugar 120.0 Starch syrup 100.0 Citric acid 4.0 Scenting agent 0.4 Ethanol extract of psyllium leaf 0.1 Finished weight 200.0 (Formulation example 2) Biscuits (g) Strong flour 100.0 Soft flour 100.0 White sucrose 30.0 Powder sugar 40.0 Shortening 100.0 Baking soda 0.6 Whole milk powder 4.0 Water 20.0 Ethanol extract of psyllium fruit 0.2 Finished weight 360.0 (Prescription example 3) Pound cake (g) Shortening 72.0 White sucrose 130.0 Water 24.0 Salt 0.7 Whole egg 110.0 Soft flour 150.0 Baking powder 3.2 Geckitz leaf heat water extract 0.3 finished weight 360.0 (formulation example 4) chewing gum (g) gum base 100.0 citric acid 1. Perfume 1.0 Murraya paniculata ethyl acetate leaf extract 0.1 finished weight 100.0 (Formulation Example 5) juice (g) frozen juice concentrate 5.0 glucose solution 10.0 L-ascorbic acid 0.02 Water 85.0 Fragrance 0.1 Water extract of Gekkittsu leaf 0.1 Finished weight 100.0

[0027]

As described above in detail, since the present invention is a food and confectionery composition characterized by containing an extract obtained from a plant of the genus Muraya as an essential component, the present invention has the following excellent effects. That is, as is apparent from the results of Test Example, Streptococcus mutans that is a cause of dental caries and periodontal disease (Streptococcus mu
tans ) and Polyphilomonas gingivalis ( Por
Phymononas gingivalis ) is mixed with an active ingredient that exhibits excellent antibacterial activity against oral bacteria, and since this active ingredient is a natural product, it is extremely safe without toxicity or allergy to the human body Even if it exists, it can be provided with peace of mind, and it has an excellent effect that it can easily prevent tooth decay and periodontal disease while continuously ingesting it as food and confectionery.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display location A23L 2/02 A61K 7/26 // A61K 7/26 31/40 ACK 31/40 ACK ADZ ADZ 35 / 78 ACKK 35/78 ACK A23L 2/00 F (72) Inventor Toshie Nakahara 3-5-20 Tagawa, Yodogawa-ku, Osaka City Inabatake Co., Ltd.

Claims (3)

[Claims] 1. A food and confectionery composition comprising an extract obtained from a plant of the genus Muraya as an essential component. 2. The plant of the genus Muraya is Geckitsu ( Murr).
aya paniculata Jack) or Murraya koenigii Spr
The food and confectionery composition according to claim 1, wherein the food and confectionery composition is eng). 3. A food and confectionery composition comprising at least one compound selected from mahanin, isomahanin and murayanol.