JPH08116887A - Method for culturing loach fry - Google Patents
Method for culturing loach fryInfo
- Publication number
- JPH08116887A JPH08116887A JP6301279A JP30127994A JPH08116887A JP H08116887 A JPH08116887 A JP H08116887A JP 6301279 A JP6301279 A JP 6301279A JP 30127994 A JP30127994 A JP 30127994A JP H08116887 A JPH08116887 A JP H08116887A
- Authority
- JP
- Japan
- Prior art keywords
- feed
- loach
- rotifer
- larvae
- brachinous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000252185 Cobitidae Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims description 9
- 238000012258 culturing Methods 0.000 title claims description 3
- 241000700141 Rotifera Species 0.000 claims description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 14
- 239000001301 oxygen Substances 0.000 claims description 14
- 229910052760 oxygen Inorganic materials 0.000 claims description 14
- 230000002000 scavenging effect Effects 0.000 claims description 10
- 239000013543 active substance Substances 0.000 claims description 7
- 238000009360 aquaculture Methods 0.000 claims description 7
- 244000144974 aquaculture Species 0.000 claims description 7
- 239000003674 animal food additive Substances 0.000 claims description 2
- 239000004480 active ingredient Substances 0.000 claims 1
- 235000021466 carotenoid Nutrition 0.000 abstract description 11
- 150000001747 carotenoids Chemical class 0.000 abstract description 11
- 210000002969 egg yolk Anatomy 0.000 abstract description 8
- 235000013345 egg yolk Nutrition 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 7
- 102000002322 Egg Proteins Human genes 0.000 abstract description 6
- 108010000912 Egg Proteins Proteins 0.000 abstract description 6
- FDSDTBUPSURDBL-LOFNIBRQSA-N canthaxanthin Chemical compound CC=1C(=O)CCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)CCC1(C)C FDSDTBUPSURDBL-LOFNIBRQSA-N 0.000 abstract description 6
- MQZIGYBFDRPAKN-UWFIBFSHSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-UWFIBFSHSA-N 0.000 abstract description 4
- 230000012010 growth Effects 0.000 abstract description 4
- OOUTWVMJGMVRQF-DOYZGLONSA-N Phoenicoxanthin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)C(=O)C(O)CC1(C)C)C=CC=C(/C)C=CC2=C(C)C(=O)CCC2(C)C OOUTWVMJGMVRQF-DOYZGLONSA-N 0.000 abstract description 3
- 235000012682 canthaxanthin Nutrition 0.000 abstract description 3
- 239000001659 canthaxanthin Substances 0.000 abstract description 3
- 229940008033 canthaxanthin Drugs 0.000 abstract description 3
- 230000012447 hatching Effects 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 abstract description 2
- 230000000366 juvenile effect Effects 0.000 description 8
- 241000251468 Actinopterygii Species 0.000 description 6
- 235000019688 fish Nutrition 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 150000002148 esters Chemical group 0.000 description 4
- KRZJRNZICWNMOA-GXSJLCMTSA-N (3s,4r)-4,8-dihydroxy-3-methoxy-3,4-dihydro-2h-naphthalen-1-one Chemical compound C1=CC=C2[C@@H](O)[C@@H](OC)CC(=O)C2=C1O KRZJRNZICWNMOA-GXSJLCMTSA-N 0.000 description 3
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 3
- 235000013793 astaxanthin Nutrition 0.000 description 3
- 239000001168 astaxanthin Substances 0.000 description 3
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 3
- 229940022405 astaxanthin Drugs 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000002778 food additive Substances 0.000 description 3
- 235000013373 food additive Nutrition 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- QXNWZXMBUKUYMD-ITUXNECMSA-N 4-keto-beta-carotene Chemical compound CC=1C(=O)CCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C QXNWZXMBUKUYMD-ITUXNECMSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 238000003911 water pollution Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000238366 Cephalopoda Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 241001517610 Funa Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 241000131739 Oncorhynchus masou rhodurus Species 0.000 description 1
- 241001282110 Pagrus major Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 235000013734 beta-carotene Nutrition 0.000 description 1
- 239000011648 beta-carotene Substances 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 235000006932 echinenone Nutrition 0.000 description 1
- YXPMCBGFLULSGQ-YHEDCBSUSA-N echinenone Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCC(=O)C1(C)C)C=CC=C(/C)C=CC2=C(C)CCCC2(C)C YXPMCBGFLULSGQ-YHEDCBSUSA-N 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000001871 ion mobility spectroscopy Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Feed For Specific Animals (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明はドジョウ仔稚魚の養殖法
及び養殖用飼料の改良に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating juvenile loach larvae and an improvement in feed for aquaculture.
【０００２】[0002]
【従来の技術】従来、ドジョウの種苗生産などの養殖で
は、人工の配合飼料や卵黄等が用いられていた。2. Description of the Related Art Conventionally, artificial mixed feed, egg yolk, etc. have been used in aquaculture such as seedling production of loach.
【０００３】[0003]
【発明が解決しようとする課題】しかしこれらの飼料で
仔稚魚を飼育した場合、歩留まりが悪いのが実情であっ
た。However, when fry larvae are bred with these feeds, the actual yield is poor.
【０００４】この発明の目的は、ドジョウの仔稚魚を飼
育する場合に、生存率が高く、歩留まりが良好であるド
ジョウ仔稚魚の養殖法及び同養殖用飼料を提供する点に
ある。An object of the present invention is to provide a method for cultivating juvenile loach larvae and a feed for the same, which has a high survival rate and a good yield when rearing larval loach larvae.
【０００５】[0005]
【課題を解決するための手段】魚類の孵化、発生、仔稚
魚の生育の過程では活性酸素が発生する。これをとり除
くには抗酸化作用物質が必要である。[Means for Solving the Problems] Reactive oxygen is generated during the process of hatching, development and growth of larvae of fish. Antioxidants are needed to remove this.
【０００６】そして、アスタキサンチンなどの活性酸素
消去作用をもつカロテノイドをマダイ、サケ、マスなど
に投与した場合、卵の孵化、発生、仔稚魚の生育が良好
になると考えられる。When a carotenoid having an active oxygen scavenging action such as astaxanthin is administered to red sea bream, salmon, trout, etc., it is considered that the hatching and development of eggs and the growth of larvae are improved.
【０００７】そのため配合飼料にアスタキサンチンなど
のカロテノイドを添加することが好ましい。Therefore, it is preferable to add carotenoids such as astaxanthin to the compounded feed.
【０００８】一方、仔稚魚の場合には、配合飼料より天
然飼料の方が与えやすく、養魚場の水質汚染も少ない。On the other hand, in the case of larvae and juveniles, natural feed is easier to feed than mixed feed and water pollution in fish farms is less.
【０００９】以上のことから、ドジョウの仔稚魚養殖に
はカロテノイドなどの抗酸化活性物質を含む微小生物
（プランクトン）が好ましい。From the above, micro-organisms (plankton) containing antioxidant substances such as carotenoids are preferable for the cultivation of loach larvae.
【００１０】そこで種々の微小生物（プランクトン）に
ついて検討したところ、ツボワムシ（学名：Ｂｒａｃｈ
ｉｎｏｕｓ ｃａｌｙｃｉｆｌｏｒｕｓ）がアスタキサ
ンチン等の活性酸素消去活性を持つカロテノイドを高含
量で含むことを見出だした。Then, when various micro organisms (plankton) were examined, the rotifer (scientific name: Brach)
It has been found that inactive calciflorus) contains a high content of carotenoids having active oxygen scavenging activity such as astaxanthin.
【００１１】さらにツボワムシを飼料としてドジョウ仔
稚魚を飼育したところ、配合飼料区、卵黄等に比べ生存
率が高く歩留まりが飛躍的に向上することが明かとなっ
た。Furthermore, when juvenile loach larvae were reared using the rotifer as feed, it was revealed that the survival rate was higher and the yield was drastically improved as compared with the mixed feed group, egg yolk and the like.
【００１２】この発明は、ツボワムシＢｒａｃｈｉｎｏ
ｕｓ ｃａｌｙｃｉｆｌｏｒｕｓを養殖用飼料として使
用することを特徴とするドジョウ仔稚魚の養殖法、及び
ツボワムシを含有することを特徴とするドジョウ仔稚魚
の養殖用飼料である。The present invention relates to the rotifer Brachino
A method for cultivating juvenile loach larvae, characterized by using us caliciflorus as a feed for aquaculture, and a feed for cultivating juvenile loach larvae characterized by containing rotifer.
【００１３】ツボワムシの含有成分であるカロテノイド
組成については表１、トコフェノール組成については表
２に示す通りである。成分の同定方法は、ツボワムシを
アセトンで抽出し、各成分をカラムクロマトグラフィー
よって分離した後、紫外、可視部吸収スペクトル（ＵＶ
−ＶＩＳ）、エレクトロインパクトマススペクトル（Ｅ
Ｉ−ＭＳ）、水素核磁気共鳴スペクトル（１Ｈ−ＮＭ
Ｒ）、円偏向二色性スペクトル（ＣＤ）によった。Table 1 shows the carotenoid composition, which is a component of the rotifer, and Table 2 shows the tocophenol composition. The components were identified by extracting the rotifer with acetone and separating each component by column chromatography, followed by UV and visible absorption spectra (UV).
-VIS), electro-impact mass spectrum (E
I-MS), hydrogen nuclear magnetic resonance spectrum ( 1 H-NM
R), circular polarization dichroism spectrum (CD).
【００１４】[0014]
【表１】 [Table 1]
【００１５】[0015]
【表２】 [Table 2]
【００１６】すなわち、表１より、カンタキサンチン、
（３Ｓ）−フェニコキサンチン、（３Ｓ、３′Ｓ）−ア
スタキサンチンなどの活性酸素消去活性を持つカロテノ
イドが主成分で含まれているという新知見を得た。That is, from Table 1, canthaxanthin,
A new finding was obtained that carotenoids having an active oxygen scavenging activity such as (3S) -phenicoxanthin and (3S, 3 ′S) -astaxanthin are contained as main components.
【００１７】さらに（３Ｓ）−フェニコキサンチン、
（３Ｓ、３′Ｓ）−アスタキサンチンは、ツボワムシ体
内では主として脂肪酸とのエステル型で存在しているこ
とが明かになった。魚類において、エステル型カロテノ
イドは、遊離型に比べ吸収され易くしかも安定である。Further, (3S) -phenicoxanthine,
It was revealed that (3S, 3'S) -astaxanthin mainly exists in the rotifer body in the ester form with fatty acids. In fish, ester type carotenoids are more easily absorbed and more stable than the free type.
【００１８】一方、活性酸素消去活性は、エステル型カ
ロテノイドの方が遊離型カロテノイドに比べ弱いが、食
物として接種した場合は、消化管内で加水分解され、遊
離型に変換されるので、生体内では遊離型と同じ活性を
示すものである。またツボワムシにはトコフェノールも
高含量で含まれており、これらの活性酸素消去活性物質
の効果も奏している。On the other hand, the active oxygen scavenging activity of the ester type carotenoid is weaker than that of the free type carotenoid, but when inoculated as a food, it is hydrolyzed in the digestive tract and converted into the free type, so that it is in vivo. It exhibits the same activity as the free form. The rotifer also contains a high content of tocophenol, and the effects of these active oxygen scavenging active substances are also exhibited.
【００１９】なお見出だされた表中のβ−カロテン、エ
キネノン、カンタキサンチン、（３Ｓ）−フェニコキサ
ンチン、（３Ｓ、３′Ｓ）−アスタキサンチンの化学構
造式は、次の通りである。なお、Ｒ＝Ｈ（遊離型）、Ｒ
＝Ａｃｙｌ（エステル型）である。The chemical structural formulas of β-carotene, echinenone, canthaxanthin, (3S) -phenicoxanthin and (3S, 3 ′S) -astaxanthin found in the table are as follows. In addition, R = H (free type), R
= Acyl (ester type).
【００２０】[0020]
【化１】 Embedded image
【００２１】[0021]
【化２】 Embedded image
【００２２】[0022]
【化３】 Embedded image
【００２３】[0023]
【化４】 [Chemical 4]
【００２４】[0024]
【化５】 Embedded image
【００２５】[0025]
【作用】本発明のドジョウ仔稚魚の養殖法は、ツボワム
シＢｒａｃｈｉｎｏｕｓ ｃａｌｙｃｉｆｌｏｒｕｓを
養殖用飼料として使用する養殖法であるため、仔稚魚を
飼育する場合に、従来の人工の配合飼料や卵黄等に比し
て、生存率が高く、歩留まりが飛躍的に向上する。The method for cultivating juvenile loach larvae of the present invention is a method for culturing larval loach, Brachinous calyciflorus, as a feed for aquaculture. The survival rate is high and the yield is dramatically improved.
【００２６】また、天然飼料であるため、仔稚魚の場合
は、人工の配合飼料に比して与えやすく、養魚場の水質
汚染も少ない。Further, since it is a natural feed, in the case of larva and fry, it is easier to feed than artificial blended feed, and the water quality of the fish farm is less polluted.
【００２７】また、天然の活性酸素消去活性物質として
用いることができ、活性酸素の作用を受けやすい高級不
飽和脂肪酸が構成要素となっている細胞膜を有する生体
にはすべて防御作用を奏し、水産、畜産飼料、食品添加
物等その有用性はきわめて大きい。具体的には、水産餌
料添加物として、ドジョウ以外に、うなぎ、いわな、あ
まご、にじます、さけ、ふな、おいかわ、うぐい、は
す、もろこ、ごりの他、鯉、鮎、やまめ等の高級淡水魚
や甲殻類に対して有用である。また、畜産餌料として、
牛、豚、鶏、やぎ、ひつじ、鴨、あひる等にも同様の効
果がある。ペット用フードへの添加も有用である。ま
た、食品に添加すれば、トコフェノール、アスコルビン
酸などと同様に、活性酸素に対し防御作用を発揮する。
各種の炎症に対しても消化器、呼吸器、循環器、脳血管
障害その他に有用である。In addition, it can be used as a natural active oxygen scavenging active substance, and it has a protective effect on all living bodies having cell membranes composed of higher unsaturated fatty acids which are susceptible to the action of active oxygen. Its usefulness such as livestock feed and food additives is extremely large. Specifically, as aquatic food additives, in addition to loach, eel, Iwana, Amago, Niji, salmon, Funa, squid, Ugui, Lotus, Moroko, Gori, Koi, Ayu, Yamame It is useful for high-grade freshwater fish and crustaceans. Also, as livestock feed,
Cow, pig, chicken, goat, sheep, duck, duck etc. have the same effect. Addition to pet food is also useful. Also, when added to food, it exerts a protective action against active oxygen like tocophenol and ascorbic acid.
It is also useful for digestive, respiratory, circulatory, cerebrovascular disorders, etc. against various inflammations.
【００２８】[0028]
【実施例】ドジョウの養殖に、従来の配合飼料、卵黄、
及びツボワムシを餌料として用いて、ドジョウの仔稚魚
の飼育１４日後の生存率を測定した。従来の配合飼料
は、日清コイ配合飼料、やまと稚魚用Ｃをドジョウの仔
稚魚１００００尾あたり約２ｇ／日の割合で給餌した。
卵黄はドジョウの仔稚魚１００００尾あたり約５ｇ／日
の割合で給餌した。なおこの卵黄は０．２ｍｍメッシュ
通過物を給餌している。ツボワムシは１０〜２０尾／ｍ
ｌの密度で給餌した。[Example] For the cultivation of loach, conventional mixed feed, egg yolk,
The survival rate of the larvae of the loach loach after 14 days of breeding was measured by using the above-mentioned and rotifer as feeds. As the conventional compounded feed, the Nissin carp compounded feed and C for Yamato fry were fed at a rate of about 2 g / day per 10,000 loach larvae.
Egg yolk was fed at a rate of about 5 g / day per 10,000 loach larvae. This egg yolk is fed with a 0.2 mm mesh passing material. 10-20 tsubo rotifers / m
They were fed at a density of 1 l.
【００２９】次の表３はその結果を示す。Table 3 below shows the results.
【００３０】[0030]
【表３】 [Table 3]
【００３１】表３に示す様に、ツボワムシを用いた場
合、配合飼料区、卵黄区に比べ、顕著な生存率（歩留ま
り）の向上が認められた。As shown in Table 3, when the rotifer was used, a remarkable improvement in the survival rate (yield) was recognized as compared with the mixed feed group and the yolk group.
【００３２】この向上は、ツボワムシに高含量で含まれ
ているカロテノイドとトコフェノール等の活性酸素消去
活性物質がドジョウの仔稚魚の発育の向上に関与してい
ると考えられる。It is considered that this improvement is due to the fact that carotenoids and active oxygen scavenging active substances such as tocophenol, which are contained in the rotifer in high content, are involved in the improvement of the growth of juvenile loach larvae.
【００３３】[0033]
【発明の効果】本発明はツボワムシを養殖用飼料として
使用するドジョウ仔稚魚の養殖法であるため、仔稚魚を
飼育する場合に、従来の人工の配合飼料や卵黄等に比し
て、生存率が高く、歩留まりが飛躍的に向上する。ま
た、天然飼料であるため、仔稚魚の場合は、人工の配合
飼料に比して与えやすく、養魚場の水質汚染も少ない。
またツボワムシから得られた活性酸素消去活性物質は天
然活性酸素消去活性物質として、水産、畜産用の飼料
や、食品添加物等の各種の用途に利用することができ
る。EFFECTS OF THE INVENTION Since the present invention is a method of cultivating juvenile loach larvae, which uses the rotifer as a feed for aquaculture, the survival rate is higher than that of conventional artificial compounded feeds and egg yolks when breeding larvae. Is high and the yield is dramatically improved. In addition, since it is a natural feed, larvae are easier to feed than artificial blended feeds, and water pollution in fish farms is small.
The active oxygen scavenging active substance obtained from the rotifer can be used as a natural active oxygen scavenging active substance for various uses such as feed for fisheries and livestock, and food additives.
Claims (4)
ｌｙｃｉｆｌｏｒｕｓを養殖用飼料として使用すること
を特徴とするドジョウ仔稚魚の養殖法。1. The rotifer Brachinous ca
A method for culturing loach larvae, which comprises using lyciflorus as a feed for aquaculture.
ｌｙｃｉｆｌｏｒｕｓを含有することを特徴とするドジ
ョウ仔稚魚の養殖用飼料。2. The rotifer Brachinous ca
A feed for aquaculture of loach larvae characterized by containing lyciflorus.
ｌｙｃｉｆｌｏｒｕｓから得られた天然活性酸素消去活
性物質。3. The rotifer Brachinous ca
A natural active oxygen scavenging active substance obtained from lyciflorus.
有効成分とする水産餌料添加物。4. An aquatic feed additive containing the active substance for eliminating natural active oxygen according to claim 3 as an active ingredient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6301279A JPH08116887A (en) | 1994-10-27 | 1994-10-27 | Method for culturing loach fry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6301279A JPH08116887A (en) | 1994-10-27 | 1994-10-27 | Method for culturing loach fry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08116887A true JPH08116887A (en) | 1996-05-14 |
Family
ID=17894918
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6301279A Pending JPH08116887A (en) | 1994-10-27 | 1994-10-27 | Method for culturing loach fry |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH08116887A (en) |
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