JPH07509819A - Low-light color imager with cooled integrating camera - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] FIELD OF THE INVENTION Low-light color imaging device with cooled integrating camera The present invention relates to a color imaging device, and more particularly, to obtain multicolor images of biological samples. This paper relates to a technology for creating color images using a low-light integral color camera.

Background of the invention There is great interest in analyzing biological samples using color imaging devices. Ru. For example, U.S. Pat. No. 5.008.185 to Bacos describes PAP staining ( Human lungs marked with photoenhancer using PAP-stain-ing technique Visually analyzing cancer specimens using an imaging device is disclosed. staining The amount of specific proteins in cells can be determined from digital grayscale images of sample cells. determined. A significant limitation of grayscale analysis is that it can only analyze a single color at a time. That's true. If the analysis uses more than one color, each color must be stained and then requires a conbount process to image the image.

For cell image analysis, there are potentials to obtain spectral and gray scale information. do. A number of colors can be applied to cell specimens using conventional staining techniques.

For example, cell specimens are stained with various colored markers and then viewed using a microscope. visually inspected. Significant benefits can be obtained by capturing a single multicolor image of a stained specimen. It will be done. Image analysis techniques can be used to analyze a single image, which allows multiple images to be analyzed. Conventional equipment for obtaining is no longer required.

Using known DNA probe technology, researchers have discovered a new class of markers called fluorescent dyes. Mosquitoes can be transported into very small structures in human biological specimens. actual , fluorescent markers are delivered to the molecular level of cells by probes. normal dye However, it is not possible to stain such small structures.

However, the small size of fluorescent markers and the luminescence process of fluorescent markers Because of this, images of specimens stained with fluorescent dyes are very dark.

To date, color cameras have been used to display and analyze colored cell images.

However, color cameras are required to obtain color images of cells stained with fluorescent materials. is not used. This is due to the low sensitivity of the camera and the staining technique. This is due to the low level of color intensity. Specimens marked with fluorescent dye Proposals have been made to capture images using an image intensifier tube. However, this method results in a monochromatic image and forgoes the great benefits gained from color images. It brings about a bad result.

Therefore, we need an imaging device that can display, analyze, and record low-light color images. It is requested that it be provided. This request is particularly important if the images to be analyzed are cell specimens. It is large when it is visualized in an enlarged manner.

Disclosure summary The present invention provides a low-light color image display for microscopic or macroscopic inspection. and methods for conducting analysis.

More specifically, it is an object of the present invention to provide a color camera for obtaining low-light color images. It is about providing.

These and other objects and special advantages of the present invention include an electronic camera capable of low-light color imaging; This is realized in an imaging device equipped with a camera. This camera uses an integral optoelectronic imaging assembly (i integrating opto-electronic imaging a ssembly), a cooling means, and a lens adapter frame. product The spectroelectronic imaging assembly scans the image and generates an image signal representative of the color intensity distribution of the scanned image. make a number. The cooling means is in thermal contact with the imaging assembly to cool the imaging assembly in the image signal. reduce noise. The cooling means is attached to the open end of the camera frame . A lens adapter frame attaches the lens adjacent to the imaging assembly. Len The adapter frame is mounted in thermal contact with the cooling means. Also, the camera , operates between the imaging assembly and the lens adapter frame to prevent condensation on the imaging assembly. It has means to prevent formation.

Electronically cooled integrating camera forms the main component of the low-light color imager do. Low-light color imaging devices include a means of holding biological samples and filtered light. an integral electronic color camera, and an image analysis device. filter type light source is arranged to illuminate the stained sample. Integral electron is back The laser is positioned to receive light from the biological sample, and the integrating photoelectric imaging device is arranged to receive light from the biological sample. , a cooling means, and a conversion means. Integral photoelectronic imager images the sample and produce an image signal responsive to the color intensity distribution of the scanned image. Cooling means (photo) The image pickup device is cooled by thermal contact with the image pickup device, and the noise of the image pickup device in item i is reduced. reduce The conversion means is connected to the camera and makes the image signals distinguishable from each other. into multiple color component signals. Finally, the image analyzer means color elements and an image reconstruction means. Color element means (the color element means is a color element means) - a camera connected to receive the component signals and to identify at least two colors; (responsive to the color selection signal) is an identification signal that distinguishes between colors in the scanned image. make.

The image reconstruction means receives the color identification signal and reconstructs an image table of multiple colors included in the image. make a mark.

The achievement of the above and other objects and features of the present invention will be further described below with reference to the accompanying drawings. It will become clear from the explanation.

Brief description of the drawing FIG. 1 is an exploded view showing a cooled delta low-light color camera according to the present invention.

FIG. 2 is an exploded view of the cooling vehicle imaging assembly of the camera shown in FIG.

FIG. 3 is an enlarged partial side sectional view showing the front end of the camera shown in FIG. 1. FIG.

Fig. 1 shows the use of the present invention for controlling the electronic cooler of the camera of Fig. 1. FIG. 2 is a schematic diagram showing a circuit.

5U is a professional drawing showing a low-light color imaging device equipped with the camera of FIG. be.

FIG. 6 is a block diagram showing in more detail the multicolor image analyzer included in the apparatus of FIG. Detailed description of preferred embodiments Sensitivity or insufficient microscopy of biological specimens marked with fluorescent dyes The application of color cameras to imaging is restricted. The inventor has proposed a model for such applications. For example, the sensitivity of a color camera is determined by a write-in transfer charge-coupled device (interline). d tran-sfer charge-coupler device (C CD) (hereinafter referred to as "integrating opto-electronic device") ``electronic device'' or simply called ``FCCD''). It is enhanced by using the light integral obtained by the imaging device.

The use of integrating optoelectronic devices should collect a larger number of photons (extending image acquisition time). This increases the sensitivity of the camera. However, integrating optoelectronic devices such as CCDs The device has special noise characteristics in the form of dark current (io), which Leaves a trajectory (artifact) in the image in the form of an image. This artifact random scattering of color tods across the image (the random scattering is caused by produced by the COD's response to ambient heat, rather than by the ) can be seen with the naked eye.

Generally, magnified visualization of cell specimens magnified through a microscope is performed using an integral color camera. Therefore, it should be obtained by taking a relatively long integration time. Pixel noise in the image is Proportional to integration time. Therefore, there is a fixed relationship between the integration time and the required S/N ratio. There is a trade-off.

The present invention is a color camera for obtaining low-light cell images colored with fluorescent markers. Thermal noise effects of the integrating optoelectronic device used in the integration process cool the device during the integration period. This is based on the inventor's important recognition that the be. Cooling the device reduces its thermal activity and therefore the image obtained by the device. thermally induced artifacts are reduced. The inventor has determined that the operation of the camera is Cooling means for the imaging device that does not impede or reduce the quality of the images obtained; and A major challenge was encountered in devising a method. Figure 1, Figure 2 and FIG. 3 shows a color camera obtained according to the invention.

FIG. 1 is an exploded view of a cooled low-light integral color camera to be used in the present invention.

A camera assembly, generally designated by the reference numeral 10, has a camera frame 12 and includes a camera frame 12. The frame 12 has five closed sides of generally rectangular cross section and one open front end. There is a section 14. Color camera frame I6 is received into camera frame I2 and mounted within the camera frame 12. The camera chassis 16 is An electronic output (not shown) receives the signal from the CCD and preferably has separate RGB components. Multiple circuit boards (e.g., integral connectors) with conventional circuits that generate Accommodates the Pu1nix Model TMC-74) equipped with 12P-02.

However, the present invention is not limited to circuits that generate such signals. It will be obvious to one skilled in the art that other forms of signals can also be used successfully. . Attached to the front end of camera frame 12 is a cooled imaging assembly 20 .

In FIG. 2, cooled imaging assembly 20 is shown in an exploded view. Figures 1 and 2 Camera IO will be described below with reference to .

The front end 14 of the camera frame 12 includes a standard electronic plug or receptacle 1. 8 is attached, the receptor receiving the metal connector of the chip carrier 24. Put it in. A integrating optoelectronic device (CCD) 28 is mounted in the recess of the chip carrier 24. and the COD is covered with a thin sheet of transparent material (not shown). of the present invention In this example, a single COD chip is used to record the image.

The metal connector of the chip carrier is normally extended for A on C0D28. Below Below, the connector with the extender is referred to as a "pin" and designated by the reference numeral 26. cover Assembly 36 attaches transparent block 34 on CCD 28 to chip carrier 24. It is equipped with a bezel 40 that can be The pins of the chip carrier 24 are received within the plug 18. Once inserted, lens adapter frame 44 is placed over imaging device 28 . Re Lens adapter frame 44 receives a conventional imaging lens 48 (shown in dashed outline). It has a threaded portion 46 for insertion.

Integrating optoelectronic device 28 is cooled by direct contact with electrothermal cooler assembly 30 . For example, as the electronic thermal cooler assembly 30, Melcor Model's The rmoelectricModule Pe1tier cooler FCO-6-66 -05L is available. The electronic thermal cooler assembly transfers heat from C0D 28 to heat sink 50. It operates in a very conventional manner. For assembly, the heat sink 50 is placed in two parallel A long slot 58 is provided in which the chip carrier 24 is inserted into the plug receptacle 1. 8, the pins 26 of the chip carrier 24 fit into the slots 58. Passed. As shown in FIG. 2, the electronic thermal cooler assembly 30 includes a tip gear rear 2. 4 and a heat sink 50.

Preferably, the heat sink 50 is made of aluminum or copper and is attached to the camera case. It has a substantially rectangular shape that corresponds to the rectangular cross-sectional shape of No. 12. heat sink has a central recess 54 shaped to receive the lens adapter frame 44. , two slots 58 are provided within the recess 54 . High heat cooling by convection Convection boats 56 are disposed on both sides of the recess 54 to increase the temperature. Convection boat 56 A fan 22 (FIG. 1) is mounted adjacent to the heat sink 50 in the vicinity of the camera case 1. It is installed within 2. Fan 22 directs air flow through convection boat 56. and serves to increase the cooling rate of the heat sink 50.

Attaching the camera chassis 1B to the inside of the camera case 12, Attaching the heat sink 50 to the front end 14 and attaching the fan 22 to the camera case 12 mounting, attaching the lens adapter frame 44 to the heat sink 50, and attaching the chip. The bezel 40 to the carrier 24 uses conventional screws (not shown).

FIG. 3 shows the lens adapter frame 44 from the heat sink 50 toward the front. FIG. 2 is a sectional view of a cooled camera according to the present invention. This is the side surface of the chip carrier 24. This is a cross section of the part immediately adjacent to .

Those skilled in the art will appreciate that cooling the chip carrier 24 and CCD 28 can condensation can occur on the image, which can seriously impair the quality of the image produced by the CCD 28. It will be appreciated that this could lead to a failure of the device. Also, Since the chip carrier 24 is also cooled, the temperature on the chip carrier 24 and its pins 26 is Condensation may also form on the camera, which may interfere with camera operation. CCD2 In order to prevent condensation on the CCD 28 and the pin 26, the present invention Therefore, maintain a dry atmosphere. This dry atmosphere maintaining means is shown in Figure 3. Ru.

As shown in FIGS. 1 to 3, the bezel 40 and the transparent block 34 are When attached to the cap carrier 24, it forms a space 60 above the CCD 28. . This configuration is typically provided to protect the C0D28 from contamination and damage. . The present invention utilizes this configuration to provide a bezel between the bezel 40 and the chip carrier 24. CC held within space 60 by gasket 32 disposed around cell 40 A dry atmosphere for D28 is obtained. The dry atmosphere does not contain any water vapor. , thus condensation on or above the CCD 28 when the CCD 28 is cooled. prevent.

The pin 26 is shaped by the inner circumference of the lens adapter frame 44 and the gasket 32. a continuum that contacts and acts between the outer periphery of the bezel 40 substantially above the periphery formed by the The provision of seal 62 provides similar protection. The seal 62 is, for example, It can be in the form of an adhesive such as cone rubber cement. Pin 26 and Another seal 6 made of adhesive is placed between the slot 58 of the seat sink 50 and the inner surface of the slot 58. 4 are provided. Seals 62, 64 are attached to the lens adapter frame 44, heater The space formed by the sink 50 and the inner surface of the slot 58 is sealed. space 66 accommodates most of the length of metal connector 26. In the space 66, there is a chip Connected onto metal connector 26 when cooled by carrier 24 or electronic thermal cooler 30. Filled with dry nitrogen to prevent exposure.

The lens adapter frame 44 is mounted in direct thermal contact with the heat sink 50. The thermal conductivity between the lens adapter 44 and the heat sink 50 is Cone rubber and emergency It is preferable to increase the temperature by strong contact. Similarly, tight contact and necessary Using rubber silicone grease, remove the electronic thermal cooler 30 from the chip carrier 24. A high thermal conductivity path is formed therethrough to the heat sink 50.

The cameras shown in Figures 1-3 are operated in a closed and controlled environment, such as dry nitrogen. Preferably, it is assembled in a glove box containing an atmosphere of like this By assembling the camera in a closed and controlled environment, as shown in Figure 3. Dry nitrogen is trapped in the space 60.66, and the dry nitrogen moves the camera into the glove box. These also occur when removed from the storage and operated in the manner contemplated by the invention. It remains in space 60.66.

The cross-sectional view in FIG. 3 shows the space between the CCD 28 and the heat sink 50, and the The high thermal conductivity path between the lens adapter frame 44 and the lens adapter frame 44 is shown. camera When activated to obtain an image on CCD 28, electronic thermal cooler assembly 30 is activated; The heat is removed from the CCD 28, that is, the C0D 28 is cooled. heat sink 50 transfers heat laterally toward the set of convection boats 56 to the left and right as viewed in FIG. guide Operation of fan 22 causes heat to be convected from heat sink 50 .

Referring again to FIG. 3, the geometry conducted from chip carrier 24 and CCD 28 is The heat may pass through the lens adapter frame 44 to the outside of the transparent window 38 of the bezel 40. is returned to the surface and radiated from the outer surface. This heat path is relatively long, so the radiated Although the amount of heat generated is relatively small, it maintains the outer surface of the transparent window 38 at a temperature higher than the dew point. , and is therefore large enough to prevent condensation on the window 38.

In another embodiment of the invention, a three CCD chip configuration is used to record images.

This 3-CCD chip configuration is shown in FIG. Separate an image into specific color components A filter 130 is installed in front of the CCD chip 132, 134, 136 to separate can be placed. Next, specific color components are sent to each COD chip. It will be done. In one embodiment, a single filter is used to divide the visible light spectrum into three regions. A filter or three-filter structure can be used. In addition, the present invention does not necessarily require the spectral It is not limited only to the visible part (but also in the ultraviolet and infrared parts of the spectrum). It also includes electromagnetic radiation. The term "light" includes all these spectral parts used as including.

Figure 4 shows the power supply circuit that controls the operation of the cooling element in the form of a block diagram. be. When switch 72 is closed, plug 70 accepts the AC input and is led to the AC socket 74. The AC socket 74 has a camera system shown in Fig. 1. The chassis main power supply (not shown) is plugged in. AC power supplied to socket 74 power to the fan 22 and electronic thermal cooler assembly 30 shown in FIGS. 1-3. It is also supplied to a second switch 76 for control. When switch 76 is closed, the convection The second ACK force is provided by a linear power supply 78. Lamp 75 is a power indicator lamp It is. Power supply 78 converts and filters the Act power to provide a +6v DC output.

The DC output is provided to a plurality of connections 84, 86, 88 of the output lug 82. child These connections are then used to power the camera's electronics. example For example, connection 84 can provide power to cooling fan 22 shown in FIG. Section 88 can supply power to electronic thermal cooler 30 shown in FIG. Open switch 80 Even if the power supplied to the fan 22 is cut off, the electronic thermal cooler assembly The power supplied to 30 is not interrupted. Thus, switch 80 has a very high When obtaining a rate image, thermo-electro cooling of the CCD 28 is required. caused by the fan 22 without blocking the ric cooling). It is possible to eliminate the vibration caused by Switch 76 switches the active cooling element of the cooled camera. At the same time, it allows the electronic components of the camera chassis to warm up. Ru.

Figure 5 shows a sample stained with fluorescent dye using the above-mentioned cooled low-light color camera. Functional analysis of image analysis equipment for creating multicolor single electron images corresponding to a microscopic visualization of It is a block diagram. General details regarding the equipment control and mechanization of the apparatus of Figure 5; For specific details of image acquisition and control, see, e.g., the aforementioned Bacos US patent. It can be understood by doing. In Figure 5, the biological specimen on the test slide is Cellular properties of the specimen can be selectively mapped with fluorescent molecules using methods known in the art. It is assumed that the color is dyed using a dyeing method. For specimen staining, Many known fluorescent dyes are used, some examples of these include (5) 6-carboxylic xifluorescein-N-hydroxysuccinimide ester and 5(6)cal Hoquirhodamine-N-hydroxysuccinimide ester.

The apparatus of FIG. 5 includes a filtered light source 90 that is adapted to provide biological information to the specimen. Emit light with only the frequencies needed to excite fluorescent dyes used to stain structures . The condensing lens 92 analyzes the stimulating light. The focus is placed on a test slide 94 supporting a biological specimen 96 to be examined. biological When the specimen 96 is excited by light of an appropriate frequency produced by the light source 90, various Preferably, the dye is dyed with one or more fluorescent dyes selected to fluoresce in the color of stomach.

Dyeing powder can be produced using a wide range of known dyes (stains) or discovered dyes. You can choose from. The dye can be fluorescently dyed using methods known in the art. Linked to light molecules. The dye favors the cell's nucleus, cytoplasm or other structures, e.g. known staining dyes, or recently discovered dyes that bind to a specific structure of interest. dyes based on monoclonal antibodies, or recently discovered, one specific DNA Combined with the sequence, it uniquely identifies whether it is a known organism or a known gene array. Any antisense DNA dye may be used.

One advantage of the present invention is that it allows a variety of different marker combinations to be used simultaneously. It is to make it possible. Also, probes (genes) can be colored in two different colors. Ru. For example, cell location can be determined using cytoplasmic dyes that bind to fluorescent colors that make up the cell's cytoplasm. Then stain one particular structure (e.g. a receptor for a given hormone) with a different fluorescent color. It can be identified by staining with an antibody dye that binds to. Dyed like this Once the colored specimen is illuminated and an image obtained, analysis of the image reveals the contact between the two colors. cells with receptors are identified, whereas cells without receptors are identified by the basic color. and will have no receptor color. Alternatively, chromosomes combine in different colors. It can be detected by staining various chromosomes with a DNA probe.

Chromosomal cross-overs will appear in image analysis as strands of discoloration.

These two examples combine spectral information obtained by multicolor fluorescent staining of specimens into a single The image shows what you get and what you like. Bacos equipment allows for multiple exposures. (once for each color) is required. You can also use separate filters for each image. must be used. In general, the surfaces of different filters do not match and each exposure Displacement occurs in the image. Before the advent of the above-mentioned imaging device of the present invention, the device shown in FIG. No device existed that could produce a single multicolor image of this type.

Continuing the explanation of FIG. 5, the lens 98 displays an enlarged image of the stained specimen 96 on the top. Focused on the lens 48 of the cooled integral low-light color camera IO configured as shown below. let The lens 98 can be composed of an Ij1 microscopic lens or a macrolens.

As mentioned above, the cooled pear color camera lO has an RGB signal 100 and a synchronization signal 10 Create a video output consisting of 2. Preferred former for images represented by RGB signals The cut is based on the well-known 525 R3170 standard timing scan format. In the future, this format will be used, for example, as a standard HDTV (high definition TV) format. - Can also be made matte.

The image signal 100 and the synchronization signal 102 are generated using multiple channels of available color identification technology. input to a color image analyzer 104. Image analyzer 104 analyzes the scanned image Preferably, the device is operative to recognize or identify the color of the image. The image analyzer 104 , is preferably programmed to allow selective determination of color. A color identification device An example is U.S. Patent No. 5.087.965, incorporated herein by reference. ) is explained in detail in

The image analyzer 104 stores a color frame image produced by the camera 10. Equipped with a storage device. The analyzer uses electronic timing to determine the camera's integration time. Equipped with equipment. The color discrimination elements of image analyzer 104 are described in U.S. Pat. No. 5.08, supra. A multi-channel device of the type disclosed in No. 7°965, wherein each channel The respective fluorescent colors resulting from different fluorescent dyes in the biological structure of specimen 96 are Multi-channel devices trained to recognize are preferred.

Figure 6 shows an image analyzer performing training on the image recognition channel. Ru. In FIG. 6, the RGB signal lOO is added to the conventional A A/D converter +10 is supplied, and the A/D converter 110 converts the instantaneous Convert analog values to digital values. First, the digital image created by the camera lO The converted image is switched to frame buffer +14 by switch 11.2. , where the scanned representation of specimen 96 is stored in a conventional format and Supplied via interface 116 to conventional multi-channel display 118 be done.

At this point, frame buffer 114 contains a multicolor image of the stained specimen. The operator can observe the colors present here. Next, the operator The color selector transmits the color selection signal via signal line 122 to color selection device 120 (which device may e.g. (This is the same as that shown in FIG. 6 of the above-mentioned U.S. Pat. No. 5,087,965.) Enter. The color selection signal 122 transmits the color component information of the bound color to the color selection device 1. 20 is used to store in memory determining the colors that are important to 20. These methods Although the harpoon is not shown in FIG. It is explained in detail in the issue. The color of interest can be displayed e.g. By moving the pointer across the image and selecting different colors from the image , selected by the user. Next, set the selection switch +12 and it will be converted, The digitized and scanned image is transferred to the now programmed color selection device 120. input. The color selection device 120 stores only the colors displayed by the color selection signal. An output in the form of a pixel representation of the image obtained by the cooled camera 10 is produced.

An image in which only the colors selected by the color selection circuit 120 are stored is stored in the frame buffer 11. 4 and output to display 118 via interface +16. Ru.

One example of staining a sample and creating a multicolor single image is described in detail below. This example is chronic myeloid leukemia (chronic myeloid leukemia). emia, CML), where CML refers to the bcr gene. is fused on chromosome 22 and the abl oncogene (oncogene) is fused on chromosome 9. It has the characteristic of being fused.

Reciprocal translocations (often referred to as the Philadelphia chromosome) account for approximately 90% of CML cases. Can be seen. The human interphase nucleus undergoes mutual translocation. In order to determine whether or not the bcr/ab1 translocation probe is introduced into the nucleus be able to. bcr/ab1 translocation DNA probe labeled with rhodamine (red) Hybridized bcr probe labeled with fluorescein (green) The hybridized abl probe and the remainder of the DNA were counterstained (cou Holding counter dye DAPI (blue) for interstaining There is. If the nucleus does not possess reciprocal translocations, the device can Create a single multicolor image containing red and green dots. However, if the nucleus is reciprocally If a translocation is present, the device displays a yellow dot or a red/green dot indicating the position of the reciprocal translocation. Create a multicolor single image containing join points.

Those skilled in the art will appreciate that the present invention may be modified with other features without departing from its spirit or essential characteristics. It will be understood that it can be embodied in a fixed form. Accordingly, the embodiments disclosed herein is considered to be illustrative in all respects and not restrictive. . The scope of the present invention is determined by the claims rather than the above description, and All changes that come within the meaning and range of equivalents are intended to be included within the scope of this invention. It is.

Continuation of front page (51) Int, C1, 6 identification symbol Internal reference number GOIN 21/64 E 9118-2JHOIR13152Z 7354-5EHO4N 5/22 5 F 7205-5C5/228 Z 7205-5C (81) Designated countries EP (AT, BE, CH, DE.

DK, ES, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE) , 0A (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, NE , S.N.

TD, TG), AT, AU, BB, BG, BR, BY.

CA, CH, CZ, DE, DK, ES, FI, GB, HU, JP, KP, KR, KZ, LK, LU, MG, MN, MW, NL, No, NZ, PL, PT, RO, R.U.

SD, SE, SK, UA, US, VN I

Claims (27)

[Claims] 1. In an imaging device for obtaining an image of a biological sample, the biological sample is stored. wherein the biological sample is stained with a plurality of fluorescent dyes; an integral electronic color camera positioned to receive light from the biological sample; And the integral color camera is scanning an image of the biological sample and generating an image signal representing a color intensity distribution of the scanned image; Integrating optoelectronic imagers to create signals and reducing imager noise in image signals. a cooling means for cooling the imaging device by making thermal contact with the imaging device; and the image signals are divided into a plurality of mutually different signals corresponding to each color of the scanned image. a conversion means connected to an imaging device for converting the color component signal into a color component signal; The imaging device further includes image analysis means, the image analysis means configured to analyze each of the scanned images. connected to receive the color component signal for producing a color identification signal for distinguishing between the colors; color element means responsive to a color selection signal for identifying at least two colors; Receiving color identification signals to create a display of an image with multiple colors contained in the image An imaging device characterized by having a connected image reconstruction means. 2. Claim characterized in that the integrating optoelectronic imaging device is a single CCD chip. The imaging device according to scope 1. 3. Claim characterized in that the integrating optoelectronic imaging device is three CCD chips. The imaging device according to scope 1. 4. Separating specific color components of the image and transmitting the separated color components to the three CCDs. said biological sample and said integral photoelectronic imaging for directing each of said chips further comprising at least one filter means disposed between the apparatus. An imaging device according to claim 3. 5. Claim 1, wherein the filter means comprises three filters. The imaging device according to item 4. 6. The filter means comprises three filters, and the filters filter the image by: Claims characterized in that each component is separated into infrared light, visible light, and ultraviolet light. The imaging device according to item 4. 7. a storage device connected to the image reconstruction means for storing a representation of the image; The imaging device according to claim 1, further comprising a stage. 8. further comprising a filtered light source arranged to illuminate the biological sample. The imaging device according to claim 1, characterized in that: 9. the filtered light source has a frequency necessary to excite the fluorescent dye; 9. The imaging device according to claim 8, wherein the imaging device emits only light. 10. Claim 1, wherein the image signal is an RGB signal. Imaging device. 11. The RGB signals are in RS170 standard timing scan format of 525 lines. 11. The imaging device according to claim 10, characterized in that the imaging device is structured in a manner as follows. 12. Particularly, the RGB signal is formatted into a standard HDTV format. 11. The imaging device according to claim 10. 13. The plurality of fluorescent dyes have at least two fluorescent colors. An imaging device according to claim 1. 14. displaying said image on a screen in two or more colors included in said image; further comprising a display means connected to the image reconstruction means for An imaging device according to claim 1, characterized in that: 15. In a method for obtaining a multicolor image of a biological sample, the biological sample is dyeing with a fluorescent dye and cooling the imager to detect the image signal produced by the imager. forming an image of the stained sample with the imaging device; An image signal representing the color intensity distribution of the surface image is generated, and the image signal is analyzed to determine the color intensity distribution of the stained image. An imaging method comprising the step of creating a display of said multicolored image of a sample. 16. selectively identifying colors in the scanned image, colors produced by fluorescent dyes; A claim further comprising the step of retaining only colors corresponding to in the image. The imaging method according to item 15. 17. The dyed product is dyed using only a color that corresponds to the color produced by the fluorescent dye. Claim 16, further comprising the step of displaying an image of the sample. Imaging method described in. 18. In an electronic camera for low-light color imaging, a frame with an open front end. and, Integration to scan an image and create an image signal representing the color intensity distribution of the scanned image an optoelectronic imaging assembly; thermally bonded to the imaging assembly to reduce noise of the imaging assembly in the image signal. cooling means for electronically cooling the imaging assembly; attached to the open front end of the frame with a lens adjacent the imaging assembly; It has a lens adapter frame for attaching the lens adapter frame. a system mounted in thermal contact with the cooling means; the imaging assembly and the lens adapter to prevent condensation on the imaging assembly; An electronic camera further comprising means for operating between the camera and the data frame. 19. The integral optoelectronic imaging assembly comprises: a charged coupled device; a chip carrier means for holding the charged coupled device; cooling means includes a plurality of connections passing through the cooling means and directing the surface image signal to an image analyzer. 19. The electronic camera according to claim 18, further comprising a lens. 20. The imaging assembly includes a plurality of charged coupled devices. The electronic camera according to item 19 of the scope of demand. 21. The connector is surrounded by a dry gas that prevents condensation on the connector. The electronic camera according to claim 19, characterized in that: 22. The cooling means a heat sink, the heat sink receiving the lens adapter frame; the heat sink and the imaging assembly; an electronic thermal cooling means attached in thermal contact with the solid body; mounted adjacent to the heat sink for cooling the heat sink by convection; at least one fan attached thereto. The electronic camera according to item 18. 23. The heat sink connects the connector from the imaging assembly to the heat sink. Equipped with multiple long slots that allow you to guide the image analyzer through the The electronic camera according to claim 22, characterized in that: 24. The connector is surrounded by a dry gas that prevents condensation on the connector. 24. The electronic camera according to claim 23, characterized in that: 25. The dew condensation prevention means a transparent block through which light forming the image passes, a transparent window and a transparent block that receives the transparent block; a cover assembly comprising a bezel for inserting the a gasket between the imaging assembly and the transparent block; A sealed space is formed between the transparent block and the imaging assembly. An electronic camera according to claim 18. 26. The sealed space contains a drying gas that prevents condensation on the imaging assembly. 26. The electronic camera according to claim 25, wherein the electronic camera contains: 27. Claim 26, wherein the dry gas is dry nitrogen. Electronic camera on board.