JPH07132078A - Culture device for fungi and algae - Google Patents
Culture device for fungi and algaeInfo
- Publication number
- JPH07132078A JPH07132078A JP28149293A JP28149293A JPH07132078A JP H07132078 A JPH07132078 A JP H07132078A JP 28149293 A JP28149293 A JP 28149293A JP 28149293 A JP28149293 A JP 28149293A JP H07132078 A JPH07132078 A JP H07132078A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- liquid
- cooler
- algae
- culture tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 32
- 241000233866 Fungi Species 0.000 title abstract 5
- 239000007788 liquid Substances 0.000 claims abstract description 50
- 238000001914 filtration Methods 0.000 claims description 27
- 230000002538 fungal effect Effects 0.000 claims description 26
- 238000010438 heat treatment Methods 0.000 claims description 26
- 238000004891 communication Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 238000001139 pH measurement Methods 0.000 claims description 3
- 239000007789 gas Substances 0.000 abstract description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 abstract description 6
- 239000001301 oxygen Substances 0.000 abstract description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 3
- 239000001569 carbon dioxide Substances 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 abstract description 3
- 230000003213 activating effect Effects 0.000 abstract 1
- 238000007599 discharging Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000012937 correction Methods 0.000 description 20
- 238000000034 method Methods 0.000 description 18
- 239000000243 solution Substances 0.000 description 18
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 15
- 238000012258 culturing Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000035622 drinking Effects 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 235000013555 soy sauce Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 235000021419 vinegar Nutrition 0.000 description 2
- 239000000052 vinegar Substances 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000002990 reinforced plastic Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、菌藻類培養のための電
子制御システム機構とpH測定システムと加熱濾過シス
テムを関連設置した菌藻類培養装置に関するものであ
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fungal algae culturing apparatus in which an electronic control system mechanism for culturing fungal algae, a pH measuring system and a heating filtration system are installed in association with each other.
【0002】[0002]
【従来の技術】クロレラ培養装置については、従来から
沢山の発明が出願されているが、飲用に適した生クロレ
ラの培養に関しては、本発明者が発明した特公昭53−
28513号が代表的である。2. Description of the Related Art Many inventions have been filed for chlorella culturing apparatus. However, for culturing fresh chlorella suitable for drinking, the invention of Japanese Patent Publication No. 53-
No. 28513 is typical.
【0003】従来の飲用に適用した生クロレラ培養装置
は、培養槽の上蓋部の一側に温風又は冷風口を、他側に
排気口を夫々設け、培養槽の内壁面の適所に所定の槽内
温度で反応せしめるサーモスタットを取り付け、培養槽
内に温風又は冷風を送り込んで、加熱又は冷却を行い、
前記サーモスタットの作動により培養液の温度を一定に
保っている。A conventional chlorella culture apparatus applied to drinking is provided with a hot air or cold air outlet on one side of an upper lid of a culture tank and an exhaust port on the other side thereof, and a predetermined position is provided at an appropriate position on an inner wall surface of the culture tank. Attach a thermostat to react at the temperature in the tank, send hot or cold air into the culture tank to heat or cool,
The temperature of the culture solution is kept constant by the operation of the thermostat.
【0004】[0004]
【発明が解決しようとする課題】前記従来技術に係る発
明にあっては、その初期目的を達成しているものの培養
液を所望する一定温度に維持するには、前記の温風又は
冷風を培養液の上面に吹き付けるだけの構成では、急激
な温度変化に対応して一定温度に調整するには時間と燃
費が嵩み効率が悪い欠点があった。In the invention according to the prior art described above, in order to maintain the culture solution at a desired constant temperature even though the initial purpose is achieved, the warm air or cold air is cultured. With the structure in which only the upper surface of the liquid is sprayed, it takes time and fuel efficiency to adjust to a constant temperature in response to a rapid temperature change, and there is a drawback that efficiency is poor.
【0005】また、これらの従来技術によるものは、ク
ロレラ培養液を飲用する目的のみに開発されているもの
である。Further, these conventional techniques are developed only for the purpose of drinking a chlorella culture solution.
【0006】本発明は、上記従来技術の問題に鑑みて、
液温の急激な変化に対しても迅速に適応することによっ
て、常に培養液を一定温度に確保することにより、一層
安定した生クロレラを含むあらゆる菌藻類の培養液を得
ると同時に、培養槽内に大気を強制的に導入し、この大
気を酸素を多量に含んだ気体に変換して排気することに
より、周囲の空気を清浄化する菌藻類培養のための電子
制御システムと、pH測定システムを採用することによ
り所望通りにpHを調整し、加熱濾過システムの併用に
より直ちに加熱と濾過を行い、菌藻類培養液を採取する
ようにした菌藻類培養装置を完成した。The present invention has been made in view of the above problems of the prior art.
By rapidly adapting to sudden changes in liquid temperature, the culture liquid is always kept at a constant temperature, so that a more stable culture liquid of all fungal algae, including raw chlorella, can be obtained, and at the same time, in the culture tank. The atmosphere is forcibly introduced into the air, the atmosphere is converted into a gas containing a large amount of oxygen, and the air is exhausted to clean the surrounding air. By adopting this method, the pH was adjusted as desired, and heating and filtration were immediately performed by the combined use of a heating filtration system to complete a fungal algae culture apparatus for collecting a fungal algae culture solution.
【0007】[0007]
【課題を解決するための手段】添付図面を参照して本発
明の要旨を説明する。The gist of the present invention will be described with reference to the accompanying drawings.
【0008】底部にヒーター1と液温センサー2と排液
部3を設け、上部に給気口4と排気口5を設け、内部に
撹拌機6を設けた容器7に透光蓋8を付設して培養槽a
を形成し、この培養槽aを内部に制御装置9とクーラー
10を備えた機台b上に設置し、前記クーラー10と容器7
の給気口4とをダクト11を介して連通状態に設け、ヒー
ター1,撹拌機6を駆動するモーター12,液温センサー
2,クーラー10,クーラー10の冷温センサー13を夫々前
記制御装置9に制御可能に接続し、前記培養槽a内にp
H測定センサー14を設け、このpH測定センサー14に接
続したpH測定計15を制御装置9に設けたことを特徴と
する菌藻類培養装置に係るものである。また、機台aに
菌藻類培養液を加熱濾過する加熱濾過処理装置cを併設
し、培養槽aの排液部3と加熱濾過処理装置cの導液部
18を導液管19により連通状態に接続し、加熱濾過処理装
置cより菌藻類培養液を採取するように構成した請求項
1記載の菌藻類培養装置に係るものである。A heater 1, a liquid temperature sensor 2, and a drainage unit 3 are provided at the bottom, an air supply port 4 and an exhaust port 5 are provided at the top, and a translucent lid 8 is attached to a container 7 having an agitator 6 inside. And culture tank a
And a control device 9 and a cooler inside the culture tank a.
Installed on the machine base b equipped with 10, the cooler 10 and the container 7
The air supply port 4 is connected to the air supply port 4 through the duct 11, and the heater 1, the motor 12 for driving the agitator 6, the liquid temperature sensor 2, the cooler 10, and the cool temperature sensor 13 of the cooler 10 are provided to the control device 9, respectively. Controllably connected, p in the culture tank a
The present invention relates to a fungal algae culture device characterized in that an H measurement sensor 14 is provided, and a pH meter 15 connected to the pH measurement sensor 14 is provided in the control device 9. In addition, a heating / filtering device c for heating and filtering the fungal algae culture solution is provided on the machine base a, and the drainage part 3 of the culture tank a and the liquid introducing part of the heating / filtering device c are installed.
The fungal-algae culture apparatus according to claim 1, wherein the fluid-conducting pipe 19 connects the 18 to each other so as to collect the fungal-algae culture solution from the heat filtration treatment apparatus c.
【0009】[0009]
【作用】実施例に基づいて実施例の項で説明する。The operation will be described based on the embodiments.
【0010】[0010]
【実施例】図面は本発明の好適な実施の一例を図示した
もので、底部にヒーター1と液温センサー2と排液部3
を設け、上部に給気口4と排気口5を設け、内部に撹拌
機6を設けた容器7に透光蓋8を付設して培養槽aを形
成し、この培養槽aを内部に制御装置9とクーラー10を
備えた機台b上に設置し、前記クーラー10と容器7の給
気口4とをダクト11を介して連通状態に設け、ヒーター
1,撹拌機6を駆動するモーター12,液温センサー2,
クーラー10,クーラー10の冷温センサー13を夫々前記制
御装置9に制御可能に接続し、前記培養槽a内にpH測
定センサー14を設け、このpH測定センサー14に接続し
たpH測定計15を制御装置9に設ける。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The drawings show a preferred embodiment of the present invention, in which a heater 1, a liquid temperature sensor 2 and a drainage part 3 are provided on the bottom.
, A supply port 4 and an exhaust port 5 are provided in the upper part, and a translucent lid 8 is attached to a container 7 having an agitator 6 inside to form a culture tank a, and the culture tank a is controlled inside. It is installed on a machine base b equipped with a device 9 and a cooler 10, and the cooler 10 and the air supply port 4 of the container 7 are provided in communication with each other through a duct 11, and a motor 12 for driving a heater 1 and a stirrer 6 is provided. , Liquid temperature sensor 2,
A cooler 10 and a cold temperature sensor 13 of the cooler 10 are controllably connected to the control device 9, respectively, a pH measuring sensor 14 is provided in the culture tank a, and a pH measuring device 15 connected to the pH measuring sensor 14 is a controlling device. It is provided in 9.
【0011】また、機台aに菌藻類培養液を加熱濾過す
る加熱濾過処理装置cを併設し、培養槽aの排液部3と
加熱濾過処理装置cの導液部18を導液管19により連通状
態に接続し、加熱濾過処理装置cより菌藻類培養液を採
取するように構成する。Further, a heating / filtering device c for heating and filtering the fungal algae culture liquid is provided on the machine base a, and the drainage part 3 of the culture tank a and the liquid guiding part 18 of the heating / filtering device c are connected to the liquid guiding pipe 19. Is connected in a communicating state by means of and the fungal algae culture solution is collected from the heating and filtration treatment apparatus c.
【0012】更に、出願人が製作テストした菌藻類培養
装置について説明すると、底部の一部を鋼板で製しヒー
ター1を付設した強化プラスチックス (FRP)製の直
径1400mmの有底短円筒状の容器7内に500リット
ルの水(水道水・天然水)を注入し、30リットルのク
ロレラ原液とサンクロゲン(醤油と酢を3:2程度で混
合したクロレラの繁殖原料混合液)100ccを注入貯液
する。Further, to explain the fungal algae culture device manufactured and tested by the applicant, a bottomed short cylinder having a diameter of 1400 mm made of reinforced plastics (FRP) with a bottom part made of a steel plate and having a heater 1 attached thereto. Inject 500 liters of water (tap water / natural water) into the container 7, and inject 30 liters of chlorella stock solution and sun crogen (mixture of chlorella breeding material that is a mixture of soy sauce and vinegar in a ratio of 3: 2) of 100 cc. To do.
【0013】次に、正逆転可能なモーター12を水平保持
板20に固定し、該水平保持板20を前記容器7の開口直径
方向に固定し、モーター12の上部にポリカーボネート製
の番傘状の透光蓋8を容器7の開口部7'を被覆する状
態に固定し、且つ、モーター12と直結した撹拌機6(図
面の場合羽根)を水没せしめ、この撹拌機6の回転によ
り培養槽aの注入液を撹拌する。この際前記ヒーター1
は1200W、機台b内に設けたクーラー10が2150
Kcal/hの性能のものを採用し、液温センサー2により
培養槽a内の温度管理を行う。Next, the forward / reverse reversible motor 12 is fixed to the horizontal holding plate 20, the horizontal holding plate 20 is fixed in the diameter direction of the opening of the container 7, and a polycarbonate umbrella-shaped transparent member is provided above the motor 12. The optical lid 8 is fixed so as to cover the opening 7'of the container 7, and the stirrer 6 (blades in the drawing) directly connected to the motor 12 is submerged in water. Stir the injection solution. At this time, the heater 1
Is 1200 W, and the cooler 10 installed in machine base b is 2150
The one having the performance of Kcal / h is adopted, and the temperature inside the culture tank a is controlled by the liquid temperature sensor 2.
【0014】更に、前記培養槽aを機台bに乗置し、ク
ーラー10と容器7の給気口4とを直径100mmφのダク
ト11で連結し、且つ撹拌機6を駆動するモーター12のコ
ード12',液温センサー2のコード2',クーラー10の電
源コード10',クーラー10の送風コード10",クーラー10
の冷温センサー13のコード13',ヒーター1の電源コー
ド1'などを制御装置9に接続する。尚、符号22は機台
bに設けた操作扉であり、該操作扉22を開けて排液部3
の排水バルブを操作するように構成する。Further, the culture tank a is placed on the machine base b, the cooler 10 and the air supply port 4 of the container 7 are connected by a duct 11 having a diameter of 100 mmφ, and a cord of a motor 12 for driving the agitator 6. 12 ', liquid temperature sensor 2 cord 2', cooler 10 power cord 10 ', cooler 10 blast cord 10 ", cooler 10
The cord 13 'of the cold temperature sensor 13 and the power cord 1'of the heater 1 are connected to the control device 9. Reference numeral 22 is an operation door provided on the machine base b, and the operation door 22 is opened to open the drainage unit 3
Configure to operate the drain valve of.
【0015】本装置を稼動すると、ダクト11より培養槽
a内へ大気と冷風を約6m3/minの条件で強制導入し、
反対側の排気口5から外部に放出する。同時に撹拌機6
の回転により容器7内の水とクロレラ原液とサンクロゲ
ンとの混合液を撹拌する。この際、モーター12は数分ご
とに正転・逆転を繰り返し、その回転数は120rpmか
ら最高回転数1200rpm迄の到達時間が25秒程度の
条件で連続運転を行う。When the apparatus is operated, the air and the cold air are forcibly introduced into the culture tank a from the duct 11 under the condition of about 6 m 3 / min,
It is discharged to the outside from the exhaust port 5 on the opposite side. Agitator 6 at the same time
The mixture of water, the chlorella stock solution, and the sun crogen is stirred by the rotation of the. At this time, the motor 12 repeats normal rotation / reverse rotation every few minutes, and the continuous operation is performed under the condition that the arrival time from the rotation speed of 120 rpm to the maximum rotation speed of 1200 rpm is about 25 seconds.
【0016】また、クーラー10の電源コード10'により
クーラー10のコンプレッサーを作動させ、このコンプレ
ッサーのON・OFFにより設定温度例えば28℃の冷
気を起生させ、クーラー10の送風コード10"でクーラー1
0内の送風機により培養槽aに冷風を強制導入し、この
冷風温度は冷温センサー13により制御装置9で制御す
る。Further, the compressor of the cooler 10 is operated by the power cord 10 'of the cooler 10, and by turning on / off the compressor, a cool air of a set temperature, for example, 28 ° C. is generated, and the blower code 10 "of the cooler 10 is used to cool the cooler 1.
Cold air is forcibly introduced into the culture tank a by the blower in 0, and the temperature of this cold air is controlled by the controller 9 by the cold temperature sensor 13.
【0017】培養槽a内の混合液は常に撹拌されながら
透光蓋8からは太陽光を、また、ダクト11からは常にク
ーラー10を介して大気が採り入れられ、更に、一日一回
100ccのサンクロゲン (醤油と酢の混合液)を投入口
21から注入する。一週間連続操業すると、1リットルの
液に対し97%が水分で3%がクロレラの濃度を有する
生クロレラ飲料原液が得られた。1ヶ月後、培養槽a内
の生クロレラ培養液を30リットル残し、再び水500
リットルを入れ、前記同様の実施を繰り返したところ同
様の結果を得た。The mixed solution in the culture tank a is constantly stirred and sunlight is taken in from the translucent lid 8 and atmospheric air is taken in from the duct 11 via the cooler 10 at a time of 100 cc once a day. Input port for sun crogen (mixed solution of soy sauce and vinegar)
Inject from 21. After continuous operation for one week, a raw chlorella beverage stock solution having a concentration of 97% water and 3% chlorella per 1 liter of liquid was obtained. After one month, 30 liters of the live chlorella culture solution in the culture tank a was left, and water 500 was added again.
The same result was obtained when liters were added and the same procedure as above was repeated.
【0018】この培養槽aは、容器7内の混合液が常に
正逆転している撹拌機6により撹拌されているので、該
混合液が常に上下入れ替わりながら導入されてくる大気
と接触し、混合液中のクロレラが大気の炭酸ガスを効果
的に吸収し、且つ、同時にクロレラが酸素を放出するこ
とになる。従って、この培養槽aは給気口4から導入さ
れた大気から炭酸ガスを吸引し、酸素を多量に含んだ気
体に変換して排気口5から放出するから周囲の大気を清
浄化する作用を発揮する。In this culture tank a, since the mixed solution in the container 7 is constantly stirred by the stirrer 6 which is rotated in the normal and reverse directions, the mixed solution always comes in contact with the introduced air while being mixed up and down to mix. Chlorella in the liquid effectively absorbs carbon dioxide in the atmosphere, and at the same time, chlorella releases oxygen. Therefore, this culture tank a sucks carbon dioxide gas from the atmosphere introduced from the air supply port 4, converts it into a gas containing a large amount of oxygen, and discharges it from the exhaust port 5, so that it has an action of cleaning the surrounding atmosphere. Demonstrate.
【0019】また、培養槽aに設けたpH測定センサー
14と制御装置9に設けたpH測定計15とを接続すること
により菌藻類培養液のpHを制御装置9の制御ソフトに
よりヒーター1による培養槽a内の加熱条件やクーラー
の送風温度を制御して常に所望のpH値の培養液が得ら
れるように調整することが可能となる。この場合、イオ
ン測定センサー16とイオン測定計17を夫々培養槽aと制
御装置9内に設けて接続して併用すれば、イオン濃度も
所望通りに調整することができる。A pH measuring sensor provided in the culture tank a
By connecting 14 with a pH meter 15 provided in the control device 9, the pH of the fungal algae culture solution is controlled by the control software of the control device 9 to control the heating conditions in the culture tank a by the heater 1 and the blower temperature of the cooler. It is possible to adjust so that a culture solution having a desired pH value can always be obtained. In this case, the ion concentration can be adjusted as desired by providing the ion measuring sensor 16 and the ion measuring meter 17 in the culture tank a and in the control device 9 and connecting them.
【0020】更に、加熱濾過処理装置cを併設し、培養
槽a内の生の培養液を培養槽aの排液部3から導液管19
を介して導液部18より加熱濾過処理装置cに導入し、直
ちに加熱(100℃以上で加熱して殺菌も兼ねるが高圧
下での瞬間加熱が望ましい)濾過して安定化した菌藻類
培養液を採取する。Further, a heating / filtering device c is provided side by side, and the raw culture liquid in the culture tank a is introduced from the liquid draining section 3 of the culture tank a into the liquid guiding tube 19
Introduced into the heating / filtering device c from the liquid introducing section 18 via the liquid, and immediately heated (heated at 100 ° C. or higher for sterilization, but instantaneous heating under high pressure is preferable), filtered and stabilized fungal algae culture liquid To collect.
【0021】尚、図中符号23は制御装置9の操作扉22に
設けた監視窓であり、24は採液部、25はフィルターであ
る。In the figure, reference numeral 23 is a monitoring window provided on the operation door 22 of the control device 9, 24 is a liquid collecting section, and 25 is a filter.
【0022】[0022]
【発明の効果】本発明は上述のように構成したから、所
望する液温を予め設定しておくことにより、周囲の気象
条件によって設定温度以上になった場合にはクーラーが
働いて瞬時に設定温度に冷やし、また、設定温度以下に
なった場合にはヒーターが働いて瞬時に設定温度に温め
る。従って、稼動中における液度は設定温度に対して常
に誤差が少なく、また、培養液も常に均一に撹拌され、
更に透光蓋が最大限太陽光を採り入れることが相俟っ
て、培養活動が頗る均一的活発になる。よって、常に均
一的に安定した菌藻類培養液が得られると同時に、極め
て多量の酸素を含んだ気体を放出できるので、汚染空気
の清浄作用も発揮し、且つ、制御装置により諸設備を関
連的に制御でき、pHも所望通りに調整可能となる。Since the present invention is configured as described above, by setting a desired liquid temperature in advance, when the temperature exceeds the set temperature due to the surrounding weather conditions, the cooler works to instantly set the temperature. When it is cooled to the temperature, and when it falls below the set temperature, the heater works to instantly warm it to the set temperature. Therefore, the liquidity during operation always has a small error with respect to the set temperature, and the culture solution is always stirred uniformly.
Furthermore, the translucent lid takes in the maximum amount of sunlight, which makes the culture activity even more active. Therefore, a uniform and stable fungal algae culture solution can always be obtained, and at the same time, a gas containing an extremely large amount of oxygen can be released, so that a contaminated air cleaning action can be exerted and various equipment can be related by a control device. The pH can be controlled as desired, and the pH can be adjusted as desired.
【0023】また、加熱濾過処理装置の併設に直ちに安
定した菌藻類培養液を採取することができる秀れた菌藻
類培養装置となる。Moreover, the excellent fungal algae culture device can be immediately installed in parallel with the heating and filtration treatment device to collect a stable fungal algae culture solution.
【図1】本発明の一実施例を示す一部を切り欠いた正面
説明図である。FIG. 1 is a partially cutaway front view showing an embodiment of the present invention.
【図2】本発明の一実施例を示す組立並びに配線状態の
簡略説明図である。FIG. 2 is a simplified explanatory view of an assembly and wiring state showing an embodiment of the present invention.
1 ヒーター 2 液温センサー 3 排液部 4 給気口 5 排気口 6 撹拌機 7 容器 8 透光蓋 9 制御装置 10 クーラー 11 ダクト 12 モーター 13 冷温センサー 14 pH測定センサー 15 pH測定計 18 導液部 19 導液管 a 培養槽 b 機台 c 加熱濾過処理装置 1 Heater 2 Liquid temperature sensor 3 Drainage part 4 Air supply port 5 Exhaust port 6 Stirrer 7 Container 8 Light-transmitting lid 9 Control device 10 Cooler 11 Duct 12 Motor 13 Cooling temperature sensor 14 pH measuring sensor 15 pH measuring meter 18 Liquid conducting part 19 Liquid transfer tube a Culture tank b Machine stand c Heat filtration treatment device
─────────────────────────────────────────────────────
─────────────────────────────────────────────────── ───
【手続補正書】[Procedure amendment]
【提出日】平成5年11月11日[Submission date] November 11, 1993
【手続補正1】[Procedure Amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】請求項1[Name of item to be corrected] Claim 1
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【手続補正2】[Procedure Amendment 2]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】0003[Name of item to be corrected] 0003
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【0003】従来の飲用に適した生クロレラ培養装置
は、培養槽の上蓋部の一側に温風又は冷風口を、他側に
排気口を夫々設け、培養槽の内壁面の適所に所定の槽内
温度で反応せしめるサーモスタットを取り付け、培養槽
内に温風又は冷風を送り込んで、加熱又は冷却を行い、
前記サーモスタットの作動により培養液の温度を一定に
保っている。A conventional chlorella culturing apparatus suitable for drinking is provided with a hot air or cold air outlet on one side of the upper lid of the culture tank and an exhaust outlet on the other side, and a predetermined position is provided at an appropriate position on the inner wall surface of the culture tank. Attach a thermostat to react at the temperature in the tank, send hot or cold air into the culture tank to heat or cool,
The temperature of the culture solution is kept constant by the operation of the thermostat.
【手続補正3】[Procedure 3]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】0006[Correction target item name] 0006
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【0006】本発明は、上記従来技術の問題に鑑みて、
液温の急激な変化に対しても迅速に適応することによっ
て、常に培養液を一定温度に確保することにより、一層
安定した生クロレラを含むあらゆる菌藻類の培養液を得
ると同時に、培養槽内に大気を強制的に導入し、この大
気を酸素を多量に含んだ気体に変換して排気することに
より、周囲の空気を清浄化する菌藻類培養のための電子
制御システムと、pH測定システムを採用することによ
り所望通りにpHを調整し、加熱濾過システムの併用に
より直ちに加熱と濾過を行い、菌藻類培養液を採取でき
るようにした菌藻類培養装置を完成した。The present invention has been made in view of the above problems of the prior art.
By rapidly adapting to sudden changes in liquid temperature, the culture liquid is always kept at a constant temperature, so that a more stable culture liquid of all fungal algae, including raw chlorella, can be obtained, and at the same time, in the culture tank. The atmosphere is forcibly introduced into the air, the atmosphere is converted into a gas containing a large amount of oxygen, and the air is exhausted to clean the surrounding air. By adopting this method, the pH was adjusted as desired, and heating and filtration were immediately carried out by the combined use of a heating filtration system to complete a fungal algae culture device capable of collecting a fungal algae culture solution.
【手続補正4】[Procedure amendment 4]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】0008[Correction target item name] 0008
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【0008】底部にヒーター1と液温センサー2と排液
部3を設け、上部に給気口4と排気口5を設け、内部に
撹拌機6を設けた容器7に透光蓋8を付設して培養槽a
を形成し、この培養槽aを内部に制御装置9とクーラー
10を備えた機台b上に設置し、前記クーラー10と容器7
の給気口4とをダクト11を介して連通状態に設け、ヒー
ター1,撹拌機6を駆動するモーター12,液温センサー
2,クーラー10,クーラー10の冷温センサー13などを夫
々前記制御装置9に制御可能に接続し、前記培養槽a内
にpH測定センサー14を設け、このpH測定センサー14
に接続したpH測定計15を制御装置9に設けたことを特
徴とする菌藻類培養装置に係るものである。また、機台
aに菌藻類培養液を加熱濾過する加熱濾過処理装置cを
併設し、培養槽aの排液部3と加熱濾過処理装置cの導
液部18を導液管19により連通状態に接続し、加熱濾過処
理装置cより菌藻類培養液を採取するように構成した請
求項1記載の菌藻類培養装置に係るものである。A heater 1, a liquid temperature sensor 2, and a drainage unit 3 are provided at the bottom, an air supply port 4 and an exhaust port 5 are provided at the top, and a translucent lid 8 is attached to a container 7 having an agitator 6 inside. And culture tank a
And a control device 9 and a cooler inside the culture tank a.
Installed on the machine base b equipped with 10, the cooler 10 and the container 7
The air supply port 4 is connected to the air supply port 4 via the duct 11, and the heater 1, the motor 12 for driving the agitator 6, the liquid temperature sensor 2, the cooler 10, the cool temperature sensor 13 of the cooler 10, etc. are respectively provided in the control device 9 Is controllably connected to the culture tank a, and a pH measuring sensor 14 is provided in the culture tank a.
The present invention relates to a fungal algae culturing device, characterized in that a pH measuring device 15 connected to the above is provided in the control device 9. In addition, a heating / filtering device c for heating and filtering the fungal algae culture solution is provided on the machine base a, and the drainage part 3 of the culture tank a and the liquid guiding part 18 of the heating / filtering device c are connected by a liquid guiding pipe 19. The present invention relates to the fungal algae culture device according to claim 1, wherein the fungal algae culture liquid is collected from the heat filtration treatment device c.
【手続補正5】[Procedure Amendment 5]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】0010[Correction target item name] 0010
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【0010】[0010]
【実施例】図面は本発明の好適な実施の一例を図示した
もので、底部にヒーター1と液温センサー2と排液部3
を設け、上部に給気口4と排気口5を設け、内部に撹拌
機6を設けた容器7に透光蓋8を付設して培養槽aを形
成し、この培養槽aを内部に制御装置9とクーラー10を
備えた機台b上に設置し、前記クーラー10と容器7の給
気口4とをダクト11を介して連通状態に設け、ヒーター
1,撹拌機6を駆動するモーター12,液温センサー2,
クーラー10,クーラー10の冷温センサー13などを夫々前
記制御装置9に制御可能に接続し、前記培養槽a内にp
H測定センサー14を設け、このpH測定センサー14に接
続したpH測定計15を制御装置9に設ける。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The drawings show a preferred embodiment of the present invention, in which a heater 1, a liquid temperature sensor 2 and a drainage part 3 are provided on the bottom.
, A supply port 4 and an exhaust port 5 are provided in the upper part, and a translucent lid 8 is attached to a container 7 having an agitator 6 inside to form a culture tank a, and the culture tank a is controlled inside. It is installed on a machine base b equipped with a device 9 and a cooler 10, and the cooler 10 and the air supply port 4 of the container 7 are provided in communication with each other through a duct 11, and a motor 12 for driving a heater 1 and a stirrer 6 is provided. , Liquid temperature sensor 2,
A cooler 10, a cool temperature sensor 13 of the cooler 10 and the like are controllably connected to the control device 9, respectively, and are placed in the culture tank a.
An H measurement sensor 14 is provided, and a pH meter 15 connected to the pH measurement sensor 14 is provided in the control device 9.
【手続補正6】[Procedure correction 6]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】0020[Correction target item name] 0020
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【0020】更に、加熱濾過処理装置cを併設し、培養
槽a内の生の培養液を培養槽aの排液部3から導液管19
を介して導液部18より加熱濾過処理装置cに導入し、直
ちに加熱(100℃以上で加熱して殺菌も兼ねるが高圧
下での瞬間加熱が望ましい)濾過(濾紙を使用する)し
て安定化した菌藻類培養液を採取する。Further, a heating / filtering device c is provided side by side, and the raw culture liquid in the culture tank a is introduced from the liquid draining section 3 of the culture tank a into the liquid guiding tube 19
Introduced into the heating / filtering device c from the liquid guiding part 18 via, and immediately heated (heated at 100 ° C. or more to double as sterilization but instantaneous heating under high pressure is preferable) and filtered (use filter paper) to stabilize Collect the transformed fungal algae culture solution.
【手続補正7】[Procedure Amendment 7]
【補正対象書類名】図面[Document name to be corrected] Drawing
【補正対象項目名】全図[Correction target item name] All drawings
【補正方法】変更[Correction method] Change
【補正内容】[Correction content]
【図1】 [Figure 1]
【図2】 [Fig. 2]
Claims (2)
を設け、上部に給気口と排気口を設け、内部に撹拌機を
設けた容器に透光蓋を付設して培養槽を形成し、この培
養槽を内部に制御装置とクーラーを備えた機台上に設置
し、前記クーラーと容器の給気口とをダクトを介して連
通状態に設け、ヒーター,撹拌機を駆動するモーター,
液温センサー,クーラー,クーラーの冷温センサーを夫
々前記制御装置に制御可能に接続し、前記培養槽内にp
H測定センサーを設け、このpH測定センサーに接続し
たpH測定計を制御装置に設けたことを特徴とする菌藻
類培養装置。1. A culture tank is formed by providing a heater, a liquid temperature sensor, and a drainage unit on the bottom, an air supply port and an exhaust port on the top, and a translucent lid attached to a container having an agitator inside. Then, this culture tank is installed on a machine stand equipped with a control device and a cooler inside, and the cooler and the air supply port of the container are provided in communication with each other through a duct, and a motor for driving a heater and a stirrer,
A liquid temperature sensor, a cooler, and a cooler temperature sensor of the cooler are controllably connected to the control device, respectively, and p
An apparatus for cultivating algae algae, comprising an H measurement sensor, and a pH meter connected to the pH measurement sensor provided in a control device.
濾過処理装置を併設し、培養槽の排液部と加熱濾過処理
装置の導液部を導液管により連通状態に接続し、加熱濾
過処理装置より菌藻類培養液を採取するように構成した
請求項1記載の菌藻類培養装置。2. A heating / filtering device for heating and filtering the fungal algae culture solution is provided on the machine base, and the drainage part of the culture tank and the liquid guiding part of the heating / filtering device are connected to each other by a liquid guiding pipe, The fungal algae culture device according to claim 1, wherein the fungal algae culture liquid is collected from the heat filtration treatment device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28149293A JPH07132078A (en) | 1993-11-10 | 1993-11-10 | Culture device for fungi and algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28149293A JPH07132078A (en) | 1993-11-10 | 1993-11-10 | Culture device for fungi and algae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07132078A true JPH07132078A (en) | 1995-05-23 |
Family
ID=17639944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP28149293A Ceased JPH07132078A (en) | 1993-11-10 | 1993-11-10 | Culture device for fungi and algae |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07132078A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100420448B1 (en) * | 2001-12-20 | 2004-02-26 | 김상길 | Liquid medium cultivator for Closterium ehrenbergii and cultivating method using the same |
| GB2378709B (en) * | 1999-12-16 | 2004-11-03 | Robert G Whiteman | Fermentation system methods and apparatus |
| US7879593B2 (en) | 1999-12-16 | 2011-02-01 | Whiteman G Robert | Fermentation systems, methods and apparatus |
| US9969633B2 (en) | 1999-12-16 | 2018-05-15 | Robert Whiteman | Systems and methods for treating oil, fat and grease in collection systems |
| WO2024122313A1 (en) * | 2022-12-05 | 2024-06-13 | 株式会社シェルタージャパン | Algae-activation-state-sustaining device |
-
1993
- 1993-11-10 JP JP28149293A patent/JPH07132078A/en not_active Ceased
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2378709B (en) * | 1999-12-16 | 2004-11-03 | Robert G Whiteman | Fermentation system methods and apparatus |
| US7879593B2 (en) | 1999-12-16 | 2011-02-01 | Whiteman G Robert | Fermentation systems, methods and apparatus |
| US9783438B2 (en) | 1999-12-16 | 2017-10-10 | Advanced Biological Services, Inc. | Apparatus for treating wastewater |
| US9969633B2 (en) | 1999-12-16 | 2018-05-15 | Robert Whiteman | Systems and methods for treating oil, fat and grease in collection systems |
| US10995024B2 (en) | 1999-12-16 | 2021-05-04 | Advanced Biological Services, Inc. | Methods of reducing the amount of settling aid |
| KR100420448B1 (en) * | 2001-12-20 | 2004-02-26 | 김상길 | Liquid medium cultivator for Closterium ehrenbergii and cultivating method using the same |
| WO2024122313A1 (en) * | 2022-12-05 | 2024-06-13 | 株式会社シェルタージャパン | Algae-activation-state-sustaining device |
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