JPH07138179A - Cell activator containing extract from belamcanda chinensis dc. and its application - Google Patents

Abstract

PURPOSE:To obtain a new cell activator capable of manifesting excellent effects, a skin external composition and a composition for bathing, containing the cell activator blended therein and useful for beauty. CONSTITUTION:This cell activator contains an extract obtained from a plant Belamcanda chinensis DC. of the family Iridaceae by using one or two or more selected from water, lower alcohols and polyol-based organic solvents. A skin external composition and a composition for bathing contain the cell activator blended therein. The extract is effective in preventing the skin from aging, improving the skin quality and curing wounds by the excellent cell activating actions.

Description

Detailed Description of the Invention

[0001] The present invention relates to a novel plant-derived cell activator and its application. More specifically, Iridaceae plants (Belamcanda chinensi)
s L.) or a dried product of the rhizome of the rhizome (crude drug name: irrigation) and a useful and highly safe cell activator and its application method.

[0002]

The field of Hyogi according to the present invention has an excellent activating effect on cells and is extremely safe. Therefore, for example, when used in combination with various external preparations such as a skin external preparation, a scalp / hair preparation, and a bath preparation, cell metabolism is activated and skin aging is prevented, or skin quality is improved and wounds are improved. A cosmetic effect such as healing can be expected. Specifically, lotion, emulsion,
Cream, oil, pack, shampoo, rinse, hairnic, hair liquid, body soap, or bath agent (liquid, powder, granule, solid, etc.
It can be applied to any). In addition to the external preparations such as those mentioned above, they can also be used by blending them with foods and drinks for maintaining health for the purpose of nutritional supplementation.

[0003]

2. Description of the Related Art Some plants have a therapeutic or inhibitory effect on various diseases, or have various useful physiological activities, and such useful plants have long been used for health maintenance. Has been widely used for. The iris of the family Iridaceae used in the present invention (the dried product of rhizome is called crude drug name: suiho) is said to have effects such as anti-inflammatory, antitussive / expectorant action, or antimicrobial action, especially sore throat and sore throat. , A plant that has been used as a folk medicine for the treatment of bronchial asthma. Ingredients contained in Hyougi include irigenin, tectorigenin, or their glycosides iridin, tectoridin, belamcandin and irisflorentin. Isoflavone compounds have been confirmed. Further, the pharmacological action is described in, for example, [Encyclopedia of Chinese Medicine, Shogakukan (1985.12.10)] and the like, and according to this, hyaluronidase (enzyme involved in type I allergy) to tectorigenin and tectoridin in Japanese cedar. It has been observed that the inhibitory action, the decoction and the infusion of Hyougi against the pathogenic dermatophytes, and the alcohol extract has a hypotensive action. Regarding the application of Hyogi, for example, use as an anti-allergic agent by the PCA reaction inhibitory action (anti-immediate allergic action) of the five isoflavone compounds in Hyogi of JP-A-63-30417,
It has been proposed that the hydroalcoholic extract disclosed in Japanese Patent Publication No. 58-11922 is applied to skin whitening cosmetics by inhibiting the tyrosinase activity.

[0004]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention Based on the usefulness of plants that have been transmitted for a long time, the elucidation of biological mechanism of action such as pharmacological action and physiological activity, and the application method based on them. Although such research has been conducted, there are still few that are actively used effectively as a form reflected in general daily life. The present inventors have searched for plant components useful for beauty and health based on the theme of cell activation, and have studied the general usage of the same. In the process, an extract of Hydrangea vulgaris superior to the glycolytic enzyme pyruvate kinase, which is directly involved in the production of ATP (adenosine triphosphate) essential for cell activation. The present invention was completed by discovering that it exhibits an active action.

[0005]

[Means for Solving the Problems] A cell is a minimum unit that constitutes a major part of each organ or tissue in an organism and mainly has those special functions, and is necessary for fulfilling that function. It has all the functions of taking in substances, synthesizing, decomposing, storing energy, discharging products based on them, and some defense mechanism, and their integrated functions. In these cells, if the division, new generation, or part of the function is reduced, the living body loses homeostasis and causes various symptoms and diseases, so research on cell aging is biological or medical. It has been promoted by a number of researchers from an academic point of view.

In particular, regarding the skin tissue, the tissue cells constituting the epidermis, dermis and subcutaneous tissue are controlled by nutrients and regulatory factors from blood, lymph, etc. To maintain the homeostasis of the living body. In other words, the epidermis plays an important role in preventing external irritation and invasion of foreign substances, and in protecting the loss of body fluids and electrolytes from the inside of the skin (barrier function), and the dermis plays an important role as a supporting tissue of the skin. Is fulfilling. However, if the metabolism of the cells that form these tissues declines due to some factor, leading to a decrease in proliferative ability and various functions, the skin loses moisture, flexibility, and elasticity, and spots and wrinkles increase, It may be easily damaged. In addition, the rate of granulation and the rate of wound healing are reduced, sometimes leading to secondary diseases.

In order for cells to perform functions such as division, proliferation, differentiation or homeostasis, at least supply of ATP which is an energy source is indispensable. The major supply of ATP in skin cells is considered to be due to glycolysis. In the glycolytic system, it is known that 11 kinds of enzymes are actually involved in the reaction from glucose to pyruvic acid and lactic acid, but it is directly involved in ATP production and is irreversible ( The enzyme involved in the reaction of phosphoenolpyruvate to pyruvate, which is the rate-determining step of glycolysis, is pyruvate kinase (PK). Therefore, based on the idea that the activation of this enzyme is greatly involved in the activation of skin cells, the PK activation action of various plant extracts was evaluated in a test system using guinea pig skin. As a result, an excellent PK activating action was observed in the extract of Hyougi, and based on this fact, the oxygen consumption test of guinea pig liver homogenate and the wound healing effect using rabbits were also examined. Therefore, the present invention was completed. Examples will be shown below to describe the present invention in more detail.

[0008]

[Examples] Pyruvate kinase (PK) activation action [Preparation of sample] Extract of 20% Ethanol of Hyogi Extract of 20% 1,3-Butylene glycol of Hyogi Extract of Ethanol, 1,3-Butylene glycol of water, water (1: 1: 3)
Extract by mixed solution (The evaporation residue of each extract is about 0.5 to 1.5%) [Animal used] Hartley female guinea pig, body weight around 300 g, 5 animals each [Test method] 1) Sample Administration Both sides of the guinea pig were shaved with hair clippers to prepare test and control groups. A lint cloth impregnated with 1 mL of an extract of Hyougi was adhered to the test section with a tape and fixed with a polyethylene film for 4 hours. The control section was similarly treated with each solvent. This operation was performed once a day for two consecutive weeks. The shearing was performed once every two days. 2) Preparation of skin extract 48 hours after the final administration of each extract of Hyougi, guinea pigs were sacrificed by bleeding and the skins of the test and control groups were separated. The obtained skin was shaved with a hair clipper to completely remove the subcutaneous tissue, accurately separated into 2 g, cut into small pieces with scissors, and sonicated by adding 20 mL of physiological saline under ice cooling. Add this suspension to 5,
After centrifugation at 000 G for 20 minutes, the supernatant was used as a skin extract. 3) Measurement of enzyme activity The following substrate mixture was prepared in advance, 4 mL of it was sampled, 100 μL of guinea pig skin extract was added to it, and the mixture was incubated at 37 ° C for 20 minutes. Substrate mixture Tris-HCl (pH = 7.5) 100mL Phospho (enol) pyruvate 3Na 0.2g MgCl ₂ 0.02g KCl 0.02g ADP 2Na 0.02g For determination, the generated pyruvic acid was reacted with 2,4-dinitrophenylhydrazine to determine the absorbance. 510 nm was measured and it calculated | required by the following formula. The enzyme activity was converted into the amount of protein determined by the Biuret method, and the activity of the test plot when the control plot was set to 100% was estimated from the T table.

[Equation 1] [Test Results] The extract of each Hyogi used in the test showed a significant difference in the enzyme activity of 113.8 to 117.3% (95% confidence interval) compared to the control group.

[0009]

[Examples] Oxygen consumption capacity test [Preparation of sample] A 20% Ethanol extract solution of Japanese cedar was concentrated and dried under reduced pressure, and 0.0001% and 0.00001% solutions were prepared with purified water. [Suspension Krebs-Ringer Bicarbonate (KBR) Adjustment] 0.90% NaCl 100 mL 1.15% KCl 4 mL 1.22% CaCl 2 3 mL 2.11% KH 2 PO 4 1 mL 3.82% MgSO 4 · 7H 2 O 1 mL 1.30% NaHCO ₃ 21 mL Total 130 mL The pH was adjusted to 7.4 with 8.5% phosphoric acid. [Preparation of Liver Homogenate] The liver of a Hartley guinea pig (female) was taken out and washed thoroughly with physiological saline, and then the same amount of KRB was added and homogenized in ice water to prepare a 50% liver homogenate. [Measurement equipment] TAIYO O ₂ UPTESTER (manufactured by Taiyo Kagaku Kogyo Co., Ltd.) [Measurement of oxygen consumption] 1.0 mL sample, KRB 2.0 m in reaction container
L, add 0.5 mL of liver homogenate. Instead of the sample, add equal amount of purified water to the control. Also, set a blank without addition of liver homogenate. On the lid of the container,
Attach filter paper moistened with CO ₂ absorbent (2N-NaOH) and seal. The container is placed in a 37 ° C. thermostat and incubated for 15 minutes, then measurement is started, and the amount of water that has moved through the capillary tube is read from the scale plate every 5 minutes. The amount of air that has moved in the capillary from the blank under the influence of atmospheric pressure is calculated, and the true oxygen consumption is calculated. [Test Results] FIG. 1 shows the changes over time in oxygen consumption. A 20% Ethanol extract of Hyougi showed an effect of increasing oxygen consumption in an oxygen consumption test using guinea pig hepatocytes. The same examination was conducted on the extracts of other solvents according to the present invention, and the effect substantially equivalent to this was obtained.

[Figure 1]

[0010]

[Examples] Wound healing effect [Preparation of test drug] Extract of 20% Ethanol of sycamore extract of Ethanol of cypress Hyogi Extract of 20% of cypress extract of 1,3-Butylene glycol Extract of cypress of 1,3Butylene glycol Liquid Extract of 20% Propylene glycol from Scutellaria barbata Ethanol, 1,3-Butylene glycol, water (1: 1: 3)
Extract by mixed solution (The evaporation residue of each extract is about 0.5 to 1.5%) Each of the above-mentioned Hyougi extracts (2 mL) was mixed with a hydrophilic ointment (10 g) to adjust. Further, as a control, one prepared by adding only each solvent was prepared. [Animal used] White Japanese rabbit, female, body weight around 3,500 g, 3 animals [Test method] The inside of the auricle of the rabbit was shaved with a depilatory cream, and 24 hours later, under anesthesia, a trepan with a diameter of 5 mm and tweezers. , 2 artificial circular ulcers with a depth of 0.2 mm using a scalpel,
Created at intervals of about 1 cm. After confirming hemostasis from the ulcer site, a sample ointment was applied to the inner surface of the left auricle, and a control ointment was applied to the inner surface of the right auricle. From the next day, the ulcer part was regularly copied on a transparent film and the area was measured by an image analyzer. still,
The ointment was applied once a day after measuring the wound. [Test Results] The area on the 10th day after the start of the test is 1 for each control group.
4.4 ~ 15.9 mm ₂ , whereas in the test area 4.8 ~ 5.7
The effect was observed for all extracts at mm ₂ .

[0011]

[Examples] Safety test 1) Primary skin irritation test Ethanol, 1,3-Butylene glycol, water (1: 1: 3)
The extract (about 1.4% as an evaporation residue) was applied to the skin of a rabbit whose back was hair-removed (3 animals per group, body weight around 3,800 g). The evaluation was performed 24, 48, and 72 hours after application by using the erythema and edema as indexes by the primary irritation scoring method. As a result, all animals were judged as negative without any erythema or edema. 2) Skin cumulative irritation test Ethanol, 1,3-Butylene glycol, water (1: 1: 3)
Once a day on the skin of the Hartley guinea pig (female, 5 animals per group, weight 320 g) with the hair removed from the flank (2 x 4 cm ₂ ). , 0.5 mL / animal was applied 5 times a week. The application was carried out for 4 weeks, and the hair removal was performed on the last application day of each week. Judgment was performed on the day after the last day of each week, using erythema and edema as an index by the primary irritation scoring method. As a result, in all animals, erythema and edema were not observed for 1 to 4 weeks after application, and it was determined to be negative. 3) Acute toxicity test The solvent was completely distilled off under reduced pressure from Ethanol extract of Scutellaria barbata and fasted for 4 hours before the test.
Oral administration of 2,000 mg / kg to a mouse, body weight 30 g) was performed, and the onset and degree of toxic symptoms were observed over time. As a result, no abnormality was observed in all mice for 14 days, and there was no abnormality in the autopsy result. The LD ₅₀ was determined to be 2,000 mg / kg or higher.

[0012]

Examples Production of various external preparations Some external preparations were produced using the cell stimulant derived from Hyogi according to the present invention. The formulation examples are shown below, but the use of the cell activating agent according to the present invention is not limited to these. 1) Production Example of Lotion A lotion was produced according to the following formulation. Weight% 1. Sorbit 2 2.1,3-butylene glycol 2 3. Polyethylene glycol 1000 1 4. Polyoxyethylene oleyl ether (25E.O.) 2 5. Ethanol 10 6. Hyougi extract (20% Ethanol ex.) 10 7. pH adjuster Appropriate amount 8. Preservative proper amount 9. Residue of purified water 100 2) Production example of emulsion An emulsion was produced according to the following formulation. Weight% 1. Squalane 3 2. Vaseline 1 3. Stearyl alcohol 0.3 4. Sorbitan monostearate 1.5 5. Polyoxyethylene (20) sorbitan monooleate 3 6.1,3-butylene glycol 5 7. Hyougi extract (20% 1,3-Butylene glycol ex.) 58. Preservative proper amount 9. Residue with purified water as 100 3) Cream production example A cream was produced according to the following formulation. Weight% 1. Squalane 20 2. Beeswax 5 3. Refined jojoba oil 5 4. Glycerin monostearate 2 5. Sorbitan monostearate 2 6. Polyoxyethylene (20) sorbitan monostearate 2 7. Glycerin 5 8. Hyougi extract (1,3-Butylene glycol ex.) 5 9. Preservative appropriate amount 10. Residue of purified water 100 4) Shampoo production example A shampoo was produced according to the following formulation. Weight% 1. Lauryl sulfate triethanolamine 5 2. Polyoxyethylene lauryl ether sulfate Na 12 3.1,3-butylene glycol 4 4. Lauric acid diethanolamide 2 5. Disodium edetate 0.1 6. Hyougi extract (20% 1,3-Butylene glycol ex.) 10 7. Preservative proper amount 8. Residue of purified water as 100 5) Hair Liquid Production Example A hair liquid was produced according to the following formulation. Weight% 1. Ethanol 29 2. Polyoxypropylene butyl ether phosphoric acid 10 3. Polyoxypropylene monobutyl ether 5 4. Triethanolamine 1 5. White cedar extract (Ethanol, 1,3-BG, water (1: 1: 3) ex.) 5 6. Preservative proper amount 7. Residue of purified water 100 6) Example of production of body soap A body soap was produced according to the following formulation. Weight% 1. Potassium laurate 15 2. Potassium myristate 5 3. Propylene glycol 5 4. Hyougi extract (20% Propylene Glycol ex.) 15 5. pH adjuster Appropriate amount 6. Preservative proper amount 7. Residue of purified water 100 7) Preparation example of bath agent (A type) A bath agent was produced according to the following formulation. Weight% 1. Sodium hydrogen carbonate 58 2. Anhydrous sodium sulfate 30 3. Borax 2 4. Dry powder of Hyougi extract (40% Ethanol ex.) 10 8) Preparation example of bath agent (B type) A bath agent was produced according to the following formulation. Weight% 1. Refined jojoba oil 5 2. Poroxyethylene sorbitan monolaurate 20 3. Glycerin monostearate 5 4. Liquid paraffin 2 5. Lauric acid diethanolamide 3 6. Hyougi extract (50% Propylene glycol ex.) 25 7. Residue of purified water 100

[0013]

[Examples] Usage test of various external preparations Male and female panelists (15 persons each) were allowed to freely use the lotions and bath agents produced in the examples for one month, and a questionnaire survey on feeling of use was requested. The results are shown in Table 1.

[Table 1]

[0014]

EFFECTS OF THE INVENTION The cell stimulant derived from Hyougi according to the present invention is excellent in its effect and highly safe. By blending this with various skin external preparations that are routinely used and using them repeatedly, cosmetic effects such as an effect of activating skin cells to improve skin quality and a wound healing effect can be obtained. That is, it is possible to maintain the original homeostasis of the skin by keeping the skin moisturized, glossy, flexible, and supple. The novel cosmetic effect of Hyogi according to the present invention and the application method based on the novel cosmetic effect are useful for human beauty and health by extremely daily methods, and also from the viewpoint of expanding effective use of plants. The effect on top is great.

[Brief description of drawings]

FIG. 1 is a graph showing the effect of 20% Ethanol extract of Hyougi on oxygen consumption in guinea pig hepatocytes.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI technical display location A61K 7/075 7/48 7/50

Claims (3)

[Claims] 1. A cell activating agent containing an extract obtained by using one or more selected from water, a lower alcohol and a polyol organic solvent from a dried product of Hydrangea vulgaris or its rhizome. 2. A composition for external application to the skin, which is obtained from a dried product of Hydrangea vulgaris or a rhizome of the family Iridaceae. 3. A bath composition containing the cell activator according to claim 1, which is obtained from a dried product of the iris family Hyougi or rhizome.