JPH0694401B2 - Fertilized egg or egg transport device - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fertilized egg or egg transporter. Furthermore, not only when it can be cryopreserved, but also when it is impossible to transport germ cells (follicle ovum, fertilized egg) or cells that are not live, or to maintain a constant temperature for a long time in a place without a power source. The present invention relates to a thermostatic device that makes it possible.

[0002]

2. Description of the Related Art Since the first report of the birth of IVF in history by Black tt et al. (1982), many researchers have actively put into practical use the IVF technology. In 1985, even in Japan, a group of Dr. Hanada of the Ministry of Agriculture, Forestry and Fisheries, Animal Husbandry Experiment Station confirmed in vitro fertilization after maturing immature follicular ova collected from ovaries of slaughtered cattle, and confirmed division. We have obtained an example in which an egg is provisionally transplanted into the oviduct of a rabbit, developed to a transplantable developmental stage, and then transplanted into a cow. This report received a great deal of attention at that time because it was possible to produce calves from the ovaries of slaughter cattle that had been discarded until now, and the ovary of slaughtered cattle was highly evaluated as a genetic resource.

[0003] In 1987, an embryo capable of transplanting an in vitro fertilized egg only by in vitro culture by a group such as Fukuda of Kitasato University and a group of Kajiwara of Kagoshima University without temporary transplantation into the oviduct of rabbits. It was reported that calves could be produced by developing to the blastocyst stage, and as a result, the cost of producing embryos derived from in vitro fertilized eggs was further reduced. However, although ovaries are now available from the slaughterhouse, ovarian intraovarian ova present in the ovaries are as early as possible after slaughter (approximately 3
Removed from ovary within time) and matured (20-24
It is known that the incidence after in vitro fertilization decreases sharply if the time is not started, and it has been impossible to transport eggs from remote areas to prescribed locations. Therefore, we examined the extraction of ovarian ova from bovine ovaries and transported them in the form of ova. Eggs in follicles that can be transplanted after in vitro fertilization can be obtained even if they are frozen and thawed after maturation culture, but it is reported that the eggs that are frozen and thawed before maturation culture hardly occur after in vitro fertilization. Therefore, it was absolutely necessary to transport these eggs while culturing them in maturation. A water bottle containing warm water is still generally used to transport eggs such as fertilized eggs that have not been frozen and stored. Conventional water bottles and incubators like this are simple ones, such as simply putting hot water in a thermos bottle, or obtaining power from an outlet or a cigarette lighter of a car, and by heating and adjusting the heater (heating wire etc.) It mainly controlled the temperature of the water in the constant temperature bath.

[0004]

The incubator and the like as described above cannot maintain a constant temperature for a long time, and those that control the temperature in the incubator tank by heating and adjusting the heater are as follows. In the case of the conventional method that requires an electric power source such as an outlet and further puts water in a constant temperature bath, when a transport container such as a test tube containing cells etc. is put in the container, the stability of the container is bad during transportation, It is difficult to carry and use in a place away from the power supply, and development of such a simple incubator has been awaited. Therefore, first of all, when it was transported using a water bottle containing warm water of 39 ° C, it was possible to obtain the same post-fertilization occurrence rate as usual, but the cultivation was continued for about 8 hours, and then the temperature of the warm water was limited. After that, the rate of occurrence decreased.

Further, the test tube containing the egg in the follicle was suspended in warm water and transported, but there were not a few eggs whose transport tube was collapsed due to vibration or the like during transport and the location of which was unknown during transport. . Next, a commercially available freeze semen thaw (manufactured by Fujidaira Kogyo Co., Ltd.), that is, a mechanism that heats the heating plate in the thermos by the thermostat control with the power source obtained from the cigarette lighter, keeps the temperature constant. We improved the melter and set the temperature to 38 ° C to transfer the ova in the follicle, but the control temperature error was extremely large (± 1 ° C).
(± 2 ° C.), and because the water was put inside the melter, the loss of the ova in the follicle occurred like the water bottle. Furthermore, there is a problem that the egg lighter cannot be entrusted to a third party such as a courier service because the cigar lighter requires a power source.

[0006]

In order to solve the above-mentioned problems, firstly, the problem of control temperature error was solved by using a semiconductor switch. Regarding the problem of the retention of the second test tube for egg transportation, it was possible to solve this problem by embedding the test tube in the particle and retaining it and using a steel ball having a high heat transfer coefficient. Third
The problem was solved by a sealed battery.

[0007]

[Function] Using the power source obtained from the built-in battery, the heater attached to the internal constant temperature bath is heated to the set temperature. The temperature is adjusted by the semiconductor switch that can turn the power on and off at the set temperature. Further, by providing a power switch so that it can be used by connecting to a cigarette lighter of an automobile, the power can be effectively used. Further, the retention of the container can be enhanced by standing a transportation container such as a test tube in a large number of steel balls (having a diameter of 1 to 2 mm). Since the heat retaining property is good, the supplemental heating after the initial temperature can obtain a stable temperature for a long time with a little electric power.

Example 1 (conventional method) The in- follicle ovum obtained from the slaughtered Japanese Black cattle was transported by truck in a conventional container for about 8 hours and then the in vitro fertilized ovum was examined for its developmental ability. It was The results are shown in Table 1.

[Table 1]

Example 2 Using the device of the present invention, in vitro fertilized egg development ability was examined in the same manner as in Example 1. The results are shown in Table 2.

[Table 2]

[0010]

[Effect] As can be seen from the results in the above table, the incidence rates at 40 hours and 8 days after fertilization have significant effects when the device of the present invention is used.

[0011]

[Brief description of drawings]

FIG. 1 is an overall view of a device of the present invention and an explanatory view showing a lid portion thereof.

FIG. 2 is a transmission diagram showing a retention state of a test tube in which a fertilized egg is received.

[Explanation of symbols]

1 constant temperature bath cover, 2 heater operation display lamp, 3 battery level indicator lamp, 4 set temperature display switch, 5 vibration setting switch, 6 power switch (for automobile cigarette lighter and built-in battery), 7 constant temperature Bath temperature display plate, 8 main switch, 9 temperature display window, 10 container lid, 11 steel ball, 12 constant temperature bath inner container, 13 thermos bottle 14 constant temperature bath outer container, 15 heater part, 16 sample (germ cell), 17 culture solution, 18 sample container.

Claims (1)

[Claims] 1. A battery is built into the inside of the body, and a heater can be heated and regulated by its power source to keep a constant temperature chamber inside the main body at a constant temperature for a long time, and an internal container such as a test tube put in the constant temperature chamber is fixed. Fertilized eggs or steel balls filled with steel balls for maintaining temperature stability and equipped with a digital temperature control mechanism that can set various temperatures
Egg transport device.