JPH0638735A - Mic data processor in dilution test on agar plate - Google Patents

Abstract

(57) [Summary] [Purpose] In the agar plate dilution test, when the minimum inhibitory concentration of a drug is to be obtained for multiple types of test bacteria, the work is less troublesome and the work can be performed easily by one person and recorded. Provided is a data processing device capable of almost eliminating the possibility of mistakes. [Structure] A data input device 20 having a plurality of dilution stage confirmation input keys 22 and designated input keys 24 arranged corresponding to a plurality of inoculation positions on an agar plate medium in a petri dish is connected to a microcomputer 10. While designating the dilution steps in order with the designated input keys, when the growth of the test bacteria is blocked for the first time at the designated dilution steps, operate the dilution stage confirmation input key corresponding to the inoculation position to The growth inhibitory dilution step for the test bacteria is determined, and the minimum growth inhibitory concentration for the test bacteria is calculated by the microcomputer based on the data.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to agar plate dilution test, which is one of the susceptibility tests of bacteria to chemotherapeutic agents. The present invention relates to a data processing apparatus capable of efficiently processing inspection data and printing the result on a recording paper when obtaining each inhibitory concentration (MIC).

[0002]

2. Description of the Related Art In the agar plate dilution method used for a chemotherapeutic agent sensitivity test, a test drug solution is serially diluted, for example, a 2-fold serially diluted drug solution of various concentrations is diluted to about 10 to 15 levels. Prepared, dispensed a fixed amount of each of the prepared diluted solutions and a fixed amount of agar in a heated and fluidized state sequentially into a petri dish, mix both in a petri dish, and then solidify and dry the agar. Then, make a series of medium containing serially diluted test drug, inoculate each plate medium with the test bacteria, store it in an incubator, incubation room, culture container, etc. for a predetermined time and test the bacteria. After culturing, by observing the presence or absence of the growth of the test bacterium, the minimum concentration of the drug inhibiting the growth, that is, the minimum inhibitory concentration (MI
I try to ask for C).

By the way, when inoculating a test bacterium to a drug-containing medium contained in a petri dish, a holding plate equipped with a handle is used to hold a plurality of inoculating rods in a hanging state. Immerse the lower end of each inoculum into each different type of bacterial solution contained in multiple small test tubes, attach a small amount of bacterial solution to the lower end of each inoculum, and then inoculate each inoculum. By simultaneously contacting the lower ends of the rods with the surfaces of the drug-containing medium contained in a single dish, it is possible to inoculate multiple sites of the drug-containing medium, for example 27 sites, with different types of test bacteria at the same time. It was And 10-15
10 to 15 agar culture mediums containing various concentrations of drug dilutions serially diluted 2-fold serially are sequentially inoculated with a plurality of types of test bacteria by the same operation as above. .

The work for obtaining the MIC value after culturing the test bacteria has been conventionally performed as follows. That is, 10 to 15 petri dishes containing agar media containing drug dilutions of various concentrations were taken out in order, and the growth status of the test bacteria at each inoculation position of the drug-containing media contained in each petri dish Was visually observed, and the results were recorded on a recording sheet as shown in FIG. 12 with a + sign when the growth of the test bacterium was observed, and a − sign when the growth of the test bacterium was not observed. Record by filling in the designated places. Then, in the series of determination results for each test bacterium, the sign is +
The MIC value for the test bacterium was determined by the drug concentration at the time when the drug changed from − to −.

[0005]

As described above, 10
It is very troublesome to record all the results of observing the presence or absence of the growth of the test bacterium at, for example, 27 inoculation positions of ˜15 drug-containing culture media. In addition, if this work is attempted by one person, it becomes very complicated, and thus it is usually necessary for two persons, a judge and a recorder, to perform the work. Furthermore, there is a risk of making a recording error by making a mistake in the entry on the recording sheet or misunderstanding.

The present invention has been made in view of the above circumstances, and reduces the troublesomeness in the work for obtaining the MIC value in the agar plate dilution test, and the work can be easily performed by one person. It is an object of the present invention to provide a data processing device which can perform recording and can almost eliminate the possibility of recording error.

[0007]

According to the present invention, an MIC data processing device in an agar plate dilution test is provided with an input means,
It is configured to include a setting data storage means, a designation input key, a dilution stage confirmation input key, a dilution stage data storage means, a computing means and a printing means. The input means, the bacterial species name of a plurality of types of test bacteria inoculated on the agar plate medium surface in a petri dish, each in association with the inoculation position of each test bacteria on the agar plate medium, respectively, also, This is for inputting the type name of the drug used, the drug concentration and the number of dilution steps, and the input setting data is stored in the setting data storage means. The designation input key has a function of designating one less dilution step each time it is operated. A plurality of the dilution stage confirmation input keys are arranged corresponding to the respective inoculation positions on the agar plate medium in the petri dish, and each dilution stage confirmation input key is inoculated into the corresponding inoculation position. The growth of the test bacterium is operated when it is first blocked in the dilution step designated by the designation input key, whereby the growth inhibition dilution step for the test bacterium is confirmed,
The input dilution step data are sequentially stored in the dilution step data storage means. Then, the calculation means calculates the minimum inhibitory concentration for each test bacterium based on the data stored in the dilution stage data storage means and the setting data storage means, respectively, and the calculated result is printed by the printing means. Is configured to be printed on recording paper.

In order to obtain MIC values for a plurality of types of test bacteria by using the data processing apparatus having the above-described structure, first, an agar medium containing a drug dilution liquid having the highest number of dilutions, that is, agar having the lowest drug concentration is used. The petri dish containing the medium is taken out, and the growth state of the test bacteria is visually observed at each inoculation position of the drug-containing medium. As a result of the observation, when the inhibition of the growth of the test bacterium was recognized even at one inoculation position, the dilution stage confirmation input key at the position corresponding to the inoculation position was operated, and the The stunting dilution stage is determined, and the input dilution stage data is stored in the dilution stage data storage means. On the other hand, when the growth of the test bacterium is observed at any of the inoculation positions, the designated input key is operated, and as described above, the dilution stage confirmation input key is operated and then the designated input key is operated. Specifies one less dilution step. Next, the petri dish containing the agar medium having the second lowest drug concentration is taken out, and the growth state of the test bacteria is visually observed at each inoculation position of the drug-containing medium. As a result of the observation, when the inhibition of the growth of the test bacteria is recognized even at one inoculation position other than the inoculation position where the dilution stage confirmation input key has already been operated, the dilution stage confirmation input at the position corresponding to the inoculation position. By operating the key, the growth-inhibiting dilution step for the test bacterium is determined, and the input dilution step data is stored in the dilution step data storage means. On the other hand, when the growth of the test bacteria is observed at any of the inoculation positions except the inoculation position at which the dilution stage confirmation input key has already been operated, operate the designated input key and confirm the dilution stage as described above. The designated input key is manipulated after the manipulation of the input key, whereby one less dilution step is designated. When the above operation is repeated for all the petri dishes, the growth-inhibiting dilution stages for each of the plurality of types of test bacteria are determined, and these are stored in the dilution-stage data storage means. Thereafter, the computing means calculates the minimum inhibitory concentration for each test bacterium based on the data stored in the dilution stage data storage means and the setting data storage means, respectively, and the calculated result is printed by the printing means. Is printed on the recording paper.

As described above, when the MIC data processing device according to the present invention is used, the MIC value for a plurality of types of test bacteria can be obtained by operating each of the plurality of dilution stage confirmation input keys once. You can

It is preferable that the dilution stage confirmation input key is constituted by a key with a built-in lamp which is turned on from being turned off by being operated. In this case, the dilution stage confirmation input key which has been operated once is mistakenly operated. Operate again,
Such an erroneous operation can be effectively prevented.

Further, in the above-mentioned MIC data processing device,
Measuring device for measuring the presence or absence of growth of the test bacteria inoculated and cultured on the agar plate medium surface in the petri dish, each visual judgment result by operating the dilution stage confirmation input key based on the visual judgment, and its visual judgment Determination data storage means for storing and accumulating the respective measurement results by the measuring device regarding the presence or absence of the growth of the test bacteria in association with each other, and the determination data stored in the determination data storage means in the determination data storage means. According to whether or not there is judgment data for the same bacterial species, selection means for selecting whether or not to perform machine judgment by the measuring device, the measurement result by the measuring device is stored in the judgment data storage means. , Data conversion means for converting into visual judgment data based on judgment data for the same bacterial species as the test bacteria, and visual judgment data converted by this data conversion means A visual judgment data storage means for sequentially storing, and a calculation means for calculating the minimum inhibitory concentration for each test bacterium based on the data respectively stored in the visual judgment data storage means and the setting data storage means. It can be made to be the composition.

In this case, once the judgment data in which the result of the visual judgment and the measurement result of the measuring device are made to correspond to each other is created and stored, the judgment data is stored in the visual judgment data storage means. For the test bacteria of the same bacterial species, the MIC value for the test bacteria can be quickly determined by the machine determination by the measuring device using the determination data stored in the visual determination data storage means without performing the visual determination one by one. It will be possible to ask for.

[0013]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT A preferred embodiment of the present invention will be described below with reference to the drawings.

1 and 2 show an embodiment of the present invention. FIG. 1 is a block diagram of an MIC data processing device, and FIG. 2 is a perspective view showing its equipment configuration. This data processing device includes a CPU (central processing unit) including a control unit, an arithmetic unit, and a register unit, a microcomputer (a microcomputer; or a personal computer (personal computer)) with a built-in memory and I / O (input / output) 10, and a keyboard. It comprises a display 12, a display 14, a printer 16, an I / O interface 18 and a data input device 20.

The data input device 20 connected to the microcomputer 10 through the I / O interface 18 has a plurality of, in this example, 27 dilution stage confirmation input keys (hereinafter abbreviated as "confirmation input keys"). 22 and a designated input key 24 are provided. 27 confirmation input keys as shown in the plan view of FIG.
Each of 22 is arranged corresponding to a plurality of, for example, 27 inoculation positions on the drug-containing agar plate medium prepared as described above and accommodated in the dish. The confirmation input key 22 is a key with a built-in lamp that is changed from a lighting state to a non-lighting state by being pressed. Then, each of the confirmation input keys 22 is initially in a lighted state in a series of operation steps, and once pressed and once changed to a light-off state, it is not operated thereafter. Each of the confirmation input keys 22 is operated when the growth of the test bacterium inoculated to the corresponding inoculation position is first stopped at a certain drug dilution stage. The function of the designated input key 24 will be described later.

An operation for obtaining the MIC value for a large number of test bacteria by using the data processing apparatus having the above-mentioned configuration for an agar plate dilution test will be described. The outline procedure of this operation is shown in FIG.
However, a series of procedures will be described in more detail in accordance with the flowchart shown in FIG.

First, according to the method described above, for example, a plurality of types of test bacteria are inoculated into each agar plate medium containing a test drug which is serially diluted by a factor of 2 in about 10 to 15 stages and cultured. Place 10-15 petri dishes on the work table. Then operate the keyboard 12,
The bacterial species name of the plurality of types of test bacteria inoculated on the agar plate medium surface in the petri dish was entered into the microcomputer 10 in association with the inoculation position of each test bacteria on the agar plate medium, and further used. The drug type name, drug concentration and number of dilution steps are input to the microcomputer 10. The input setting data are stored in the memory of the microcomputer 10. At this point, all the confirmation input keys 22 of the data input device 20 are turned on.

Next, from 10 to 15 petri dishes,
Remove the agar medium containing the drug dilution solution with the highest number of dilutions, that is, the dish containing the agar medium with the lowest drug concentration, and visually observe the growth state of the test bacteria at each inoculation position of the drug-containing medium. , Determine the presence or absence of growth of the test bacteria. As a result, when the inhibition of the growth of the test bacteria is observed even at one inoculation position, the enter input key 22 at the position corresponding to the inoculation position is operated to confirm the growth inhibition dilution step for the test bacteria. And enter. The input dilution step data is stored in the memory of the microcomputer 10. At this time, the lamp of the confirmed input key 22 operated is extinguished. On the other hand, when the growth of the test bacteria is recognized at any of the inoculation positions, the designated input key 24 is operated, and the designated input key 24 is operated after the confirm input key 22 is operated as described above, Specify one lesser dilution step and input it to the microcomputer 10. Next, the petri dish containing the agar medium with the second lowest drug concentration was taken out, and the growth state of the test bacteria was visually observed at each inoculation position of the drug-containing medium to determine the presence or absence of growth of the test bacteria. To do. As a result,
If the growth of the test bacteria is blocked even at one inoculation position other than the inoculation position corresponding to the confirmation input key 22 whose lamp is off, operate the confirmation input key 22 at the position corresponding to that inoculation position. Then, determine and enter the stunting dilution step for the test organism. The input dilution stage data is stored in the memory of the microcomputer 10, and the operated lamp of the confirm input key 22 is turned off. On the other hand, when growth of the test bacteria is observed at any of the inoculation positions except the inoculation position corresponding to the confirmation input key 22 where the lamp is off, operate the designated input key 24, and as described above. After the confirmation input key 22 is operated, the specification input key 24 is operated to further specify the dilution step one less and input it to the microcomputer 10. By repeating the above operation for all petri dishes, the growth-inhibiting dilution step is determined for all of the plurality of types of test bacteria, and these are stored in the memory of the microcomputer 10. After that, the calculation unit of the microcomputer 10 calculates the MIC value for each test bacterium based on the setting data and the dilution stage data stored in the memory. The calculated result is printed on the recording paper by the printer 16, a report is created, and the calculated result is written on the floppy disk 26.

6 and 7 show another configuration example of the present invention, FIG. 6 is a block diagram of the MIC data processing device, and FIG. 7 is a perspective view showing the device configuration thereof. This data processing device has a configuration in which a measuring device 30 is connected to a microcomputer 10 of the data processing device shown in FIGS. 1 and 2 via an I / O interface 28. Measuring device 30
Comprises a plurality of pairs of light-emitting elements and light-receiving elements arranged corresponding to 27 inoculation positions on the agar plate medium,
The presence or absence of growth of the test bacterium is determined based on the magnitude of photoelectric output from each light receiving element.

The operation for obtaining the MIC value in the agar plate dilution test will be described using the data processing apparatus having the configuration shown in FIGS. 6 and 7. In this apparatus, two kinds of operations are performed. One of the operations is an operation of judging the presence or absence of the growth of the test bacterium both by visual inspection and measurement by the measuring device 30, and measuring the MIC value and accumulating the judgment data, and the other one operation. Is an operation for automatically obtaining the MIC value by machine determination by the measuring device 30 using the accumulated determination data. The outline procedure of the former operation is shown in FIG. 8 and the outline procedure of the latter operation is shown in FIG. 9, and the series of steps will be described in more detail according to the flowcharts shown in FIGS. 10 and 11.

First, the operation for measuring the MIC value and accumulating the determination data according to the flow chart shown in FIG. 10 will be described. This operation is the above-mentioned operation using the data processing device shown in FIGS. 1 and 2. And the basic operation is the same, except that the measuring device 30 performs machine determination after operating the designated input key 24. That is, in this device, after operating the designation input key 24 and designating the dilution step and inputting to the microcomputer 10, the petri dish containing the agar plate medium for which visual determination has been completed is set in the measuring device 30, and the measuring device is set. The growth state of the test bacterium is measured at each inoculation position on the agar plate medium by 30 and the measurement result is input to the microcomputer 10 and stored in the memory. And after all the petri dishes have been judged,
Each visual judgment result and each mechanical measurement result corresponding thereto are accumulated in the floppy disk 26 as judgment data.

Next, the operation for automatically obtaining the MIC value by machine determination will be described according to the flow chart shown in FIG. The data input device 20 is not used at all in this operation. First, the keyboard 12 is operated to input to the microcomputer 10 the bacterial species names of a plurality of types of test bacteria inoculated on the surface of the agar plate medium in the petri dish. Based on this input
The microcomputer 10 searches the determination data stored in the floppy disk 26 for the determination data regarding the same bacterial species as the test bacterium, and displays the result on the display 14. Then, according to the result, it is selected whether to perform the automatic measurement by the machine determination as it is or to shift to the above-described operation of storing the determination data together with the measurement of the MIC value. When the automatic measurement flow based on the machine determination is selected, the keyboard 12 is further operated to input the type name of the drug used, the drug concentration and the number of dilution steps to the microcomputer 10. Next, one of the prepared 10 to 15 petri dishes is taken out, the petri dish is set in the measuring device 30, and the growth of the test bacteria at each inoculation position of the drug-containing medium contained in the petri dish is taken out. The state is measured by the measuring device 30, and the measurement result is converted into visual judgment data (+ or −) based on the judgment data for the same bacterial species as the test bacteria, and the converted visual judgment data is used by the microcomputer 10 Stored in memory. Subsequently, the next petri dish is taken out, the same machine determination is performed, and the result is stored in the memory of the microcomputer 10. The above operation is repeated for all petri dishes, and then the microcomputer 10 calculates the MIC value for each test bacterium based on the data stored in the memory in the microcomputer 10, and the printer 16 records the result. The calculation result is written on the floppy disk 26 while being printed on a paper to create a report.

The MIC data processing apparatus according to the present invention has the above-mentioned configuration, but the scope of the present invention is not limited by the above description and the contents of the drawings, and various modifications are possible without departing from the scope of the invention. Can be included. For example, instead of using the built-in lamp key as the dilution stage confirmation input key, the key and the lamp may be separately provided and the lamp may be arranged in the vicinity of the key. Further, by providing four dilution stage confirmation input keys at each inoculation position on the agar plate medium, the growth state of the test bacteria can be changed to +++, ++, +,-.
It is also possible to adopt a configuration that can be displayed in stages.

[0024]

Since the present invention is constructed and operates as described above, in the agar plate dilution test, the MIC value for each of a plurality of types of test bacteria is determined using the data processing device according to the present invention. When you do
The annoyance in the work for that purpose is reduced, and even one person can easily perform the work, and the possibility of causing a recording error is almost eliminated.

Further, when the dilution stage confirmation input key is constituted by the lamp built-in key as described in claim 2, it is possible to effectively prevent the erroneous operation of the dilution stage confirmation input key. Further, when the constitution as described in claim 3 is adopted, the MI of the test bacteria of the same bacterial species as the bacteria for which visual judgment is made once and judgment data is created
The C value can be quickly obtained.

[Brief description of drawings]

FIG. 1 is a block diagram of a MIC data processing device showing an embodiment of the present invention.

FIG. 2 is a perspective view showing a device configuration of the MIC data processing device shown in FIG.

FIG. 3 is a plan view showing an example of a configuration of a data input device used in this MIC data processing device.

FIG. 4 is a flowchart showing a schematic procedure of an operation for obtaining MIC values for a large number of test bacteria by using this MIC data processing device.

FIG. 5 is a flow chart for explaining a series of procedures in an operation of obtaining MIC values for a large number of test bacteria by using this MIC data processing device.

FIG. 6 is a block diagram of a MIC data processing device showing another configuration example of the present invention.

7 is a perspective view showing a device configuration of the MIC data processing device shown in FIG.

FIG. 8 is a flowchart showing a schematic procedure of an operation of accumulating determination data together with measurement of MIC value.

FIG. 9 is a flowchart showing a schematic procedure of an operation for automatically obtaining an MIC value by machine determination by a measuring device using accumulated determination data.

FIG. 10 is a flowchart for explaining a series of procedures in an operation of accumulating determination data together with measurement of an MIC value.

FIG. 11 is a flowchart for explaining a series of procedures in an operation of automatically obtaining a MIC value by machine determination by a measuring device using accumulated determination data.

FIG. 12 shows an example of a recording sheet recording the results of visual observation of the presence or absence of growth of test bacteria in the conventional work for obtaining MIC values for a large number of test bacteria in the agar plate dilution test. It is a partial top view.

[Explanation of symbols]

 10 Microcomputer 12 Keyboard 14 Display 16 Printer 20 Data input device 22 Dilution step confirmation input key 24 Designated input key 26 Floppy disk 30 Measuring device

Claims (3)

[Claims] 1. The bacterial species names of a plurality of types of test bacteria inoculated on the surface of an agar plate medium in a petri dish are input in association with the inoculation position of each test bacteria on the agar plate medium, and used. The input means for inputting the type name of the drug, the drug concentration and the number of dilution steps, the setting data storage means for storing the setting data input by this input means, and one less dilution step for each operation. A plurality of designated input keys to be designated and a plurality of corresponding inoculated positions on the agar plate medium in the petri dish are respectively arranged, and the corresponding inoculated positions are inoculated for the first time in the dilution step designated by the designated input keys. By operating when the growth of the tested bacteria is stopped, a dilution step confirmation input key that confirms the growth-prevention dilution step for the tested bacteria, and Based on the data stored in the dilution stage data storage means and the setting data storage means, respectively. MIC data processing device in agar plate dilution test, comprising arithmetic means for calculating the minimum inhibitory concentration for each test bacterium and printing means for printing the results calculated by this arithmetic means on recording paper . 2. The MI in the agar plate dilution test according to claim 1, wherein the dilution stage confirmation input key is a key with a built-in lamp which is turned on from being turned off by being operated.
C data processing device. 3. A measuring device for measuring the presence or absence of growth of a test bacterium inoculated and cultured on an agar plate medium surface in a petri dish, each visual judgment result by operating a dilution stage confirmation input key based on visual judgment, Judgment data storage means for storing and accumulating the respective measurement results by the measuring device regarding the presence or absence of the growth of the test bacterium judged visually, among the judgment data accumulated in the judgment data storage means. In accordance with whether or not there is judgment data for the same bacterial species as the test bacteria, the selection means for selecting whether to perform the mechanical judgment by the measuring device, the measurement result by the measuring device, the judgment data storage means Data conversion means for converting visual judgment data based on the judgment data for the same bacterial species as the test bacterium stored in the visual judgment converted by the data conversion means. Visual judgment data storage means for sequentially storing constant data, and calculation means for calculating the minimum inhibitory concentration for each test bacterium based on the data respectively stored in the visual judgment data storage means and the setting data storage means. The MIC data processing device in the agar plate dilution test according to claim 1 or 2, further comprising: