JPH06226055A - Hermetic suction type ultrafilter type sample concentrating implement - Google Patents

Abstract

PURPOSE:To provide the hermetic suction type ultrafilter type sample concentrating implement which is small in the size and weight of the body, does not require a power source, eliminates a possibility of scattering of the sample to the outside and, therefore, has excellent practicability and environmental maintenance characteristic. CONSTITUTION:This implement has an ultrafilter membrane 2 which is movable within the concentrating implement body 1 along a central axial line, a vacuum evacuation port 5 of a septum seal type and sample injecting and discharging port 6 respectively installed to the front end face and flank of the concentrating implement body 1 and a hand pump 8 connected to the front end of a connecting tube 04.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a closed suction type ultrafiltration type sample concentrator.

[0002]

2. Description of the Related Art Conventionally, for example, a centrifuge is known as a means for concentrating an experimental sample in a ground laboratory. Further, as the ultrafiltration-type experimental sample concentrating means, in the case of a single sample, as shown in the longitudinal sectional view of FIG. 02 and the ultrafiltration membrane 03 immersed in this
And the gas-liquid separator 0 inserted in the connecting tube 04 connected to this and extending through the lid to the outside of the concentrating device body 01.
5 and a vacuum pump connected to the tip of the connection tube 04 are known. Further, as a sample concentrating means for the ultrafiltration experiment, when a large amount of sample is used, one conical flask 06 for accumulating the concentrating sample 02 is provided in this, as shown in the vertical sectional view of FIG. 3 (B). The immersed ultrafiltration membrane 03, a connection tube 09 that connects the ultrafiltration membrane 03 with the plug center hole 08 of the other Erlenmeyer flask 07, and a gas-liquid inserted into the connection tube 010 that is connected to the outlet of the Erlenmeyer flask 07 What is formed from the separator 05 and the vacuum pump connected to the front end of the connection tube 010 is known.

However, these means have the following drawbacks when they are applied to experiments in space. (1) If a centrifuge is used, the means will be large and heavy. It is also difficult to supply a large amount of power. (2) In the case of using the ultrafiltration membrane 03, the sample for concentration 0
When 2 is filled in the concentrating device body 01 by opening the lid, the concentrating sample may scatter to the outside, which contaminates the spacecraft laboratory, which is a closed space, and is therefore considered for the conservation of the living environment. There is room.

[0004]

SUMMARY OF THE INVENTION The present invention has been proposed in view of the above circumstances and has a main body that is small and light in weight, does not require a power source, and has no fear of scattering a sample to the outside. Therefore, it is an object of the present invention to provide a closed suction type ultrafiltration type sample concentrating instrument excellent in practicality and environmental conservation.

[0005]

To this end, the present invention provides a closed concentrating instrument body capable of containing a concentrating sample,
An ultrafiltration membrane disposed inside the concentrating device body, a tube extending from the ultrafiltration membrane to the outside of the concentrating device body in a fluid-tight manner, a gas-liquid separator inserted into the tube, and the tube. In a closed type ultrafiltration type sample concentrator having a vacuuming means connected to the tip of, an ultrafiltration membrane movable along the central axis of the concentrating instrument body,
The concentrating device main body is equipped with a septum seal type vacuuming port, a sample pouring port, and a manual pump connected to the tip of the tube.

[0006]

According to this structure, the ultrafiltration membrane movable along the central axis of the concentrating device body, and the septum-sealed vacuum suction ports attached to the tip and side surfaces of the concentrating device body, respectively. Since the sample injection / exhaust port and the manual pump connected to the tip of the tube are provided, the following actions are performed. (1) Concentration is ultrafiltration, and the equipment is compact and lightweight. (2) The pump that decompresses the inside of the ultrafiltration membrane is a manual type and does not require a power source, and the equipment is small and lightweight. (3) The vacuum suction port and sample injection / exhaust port of the instrument body are septum seals, respectively, so that the sample does not leak outside during depressurization and sample injection / exhaust operations of the ultrafiltration membrane.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to the drawings. The same reference numerals as those in FIG. 3 denote the same members as in the same drawing. First, in the longitudinal sectional view of FIG. 1, 1 is a relatively large vertical cylinder. A relatively compact vertical cylindrical ultrafiltration membrane 2 is coaxially and vertically movable inside the main body of the concentrating instrument. Reference numeral 3 is a connection tube having a lower end connected to the upper end of the ultrafiltration membrane 2, a central portion penetrating the top plate of the concentrating instrument body 1 slidably in a fluid-tight manner and an upper portion extending to the outside. 4 is a piston for guiding the vertical movement of the connecting tube 3 and the ultrafiltration membrane 2 inside the concentrating device body 1, 5, 6
Are a vacuum suction port and a sample injection / exhaust port, which are screwed to the lower side surface and the lower end surface of the concentrating device body 1, respectively, and are sealed from the outside by a septum seal 7. 8 is connection tube 0
4 is a manual pump connected to the tip of 4.

To concentrate a sample in such an instrument, first, as shown in the longitudinal sectional view of FIG. 2 (1), the sample 02 for concentration is transferred from the culture chamber 9 to the sample cassette 10 of the syringe type. Next, as shown in the vertical cross-sectional view of FIG. 2B, the air inside the concentrating device body 1 is evacuated to the vacuum port 5
The inside of the concentrating device main body 1 is evacuated by using the air bleeding syringe 11 to evacuate. Then, the ultrafiltration membrane 2 descends with the piston 4 to the broken line position (FIG. 1). Further, as shown in the vertical sectional view of FIG. 3 (3), the sample cassette 10 containing the concentration sample 02 is connected to the sample injection / discharge port 6 of the concentration instrument body 1. Subsequently, as shown in the vertical sectional view of FIG. 4 (4), the sample 02 for concentration is injected from the sample cassette 10 into the concentration device main body 1. Then, the ultrafiltration membrane 2
Moves up with the piston 4 to the solid line position (FIG. 1).
Then, as shown in the vertical cross-sectional view of FIG. 5 (5), when the pressure inside the ultrafiltration membrane 2 is lowered by using the manual pump 8,
The concentration sample 02 is filtered by the ultrafiltration membrane 2, and the water and the like inside this reach the gas-liquid separator 05 through the connection tube 3.
Water is stopped here and only gas goes out. As a result, only water is accumulated inside the ultrafiltration membrane 2, and therefore the concentration sample 02 is collected outside the ultrafiltration membrane 2.
Will be concentrated. Finally, as shown in the longitudinal sectional view of FIG. 6 (6), the concentrated concentration sample 02 is discharged from the sample injection / discharge port 6 of the concentration instrument body 1 into the sample cassette 10. Then, the ultrafiltration membrane 2 descends together with the piston 4 to the broken line position (FIG. 1).

According to the instrument of this embodiment, an ultrafiltration membrane movable along the central axis of the concentrating instrument body,
Since the septum-sealed vacuum suction port, the sample injection port, and the manual pump connected to the tip of the tube are provided on the tip and side of the concentrator body, respectively, the following effects are achieved. To be (1) Concentration is ultrafiltration, and the equipment is small and lightweight, thus improving the practicality. (2) The pump that decompresses the inside of the ultrafiltration membrane is a manual type and does not require a power source, and the device is small and lightweight, thus improving the practicality. (3) The vacuum port and the sample injection / exhaust port of the instrument body are septum seals, respectively, so that the sample does not leak to the outside during the depressurization operation and sample injection / exhaust operation of the ultrafiltration membrane, and therefore environmental conservation It is improved, and especially suitable for space experiments.

[0010]

In summary, according to the present invention, a closed-type concentrating instrument body capable of accommodating a concentrating sample, an ultrafiltration membrane disposed inside the concentrating instrument body, and the ultrafiltration membrane as described above are used. In a closed ultrafiltration sample concentrator having a tube extending fluid-tight to the outside of the main body of the concentrating instrument, a gas-liquid separator inserted into the tube, and a vacuuming means connected to the tip of the tube, An ultrafiltration membrane movable along the central axis of the concentrating device body, a septum seal type vacuuming port, a sample pouring port, attached to the tip surface and the side surface of the concentrating device body, and the tube of the tube. By having a manual pump connected to the tip, the main body is compact and lightweight, does not require a power source, and there is no fear that the sample will scatter to the outside, therefore it is practical and environmentally friendly. Since obtaining a closed suction ultrafiltration type sample concentration device was, the present invention is extremely useful industrially.

[Brief description of drawings]

FIG. 1 is a vertical sectional view showing an embodiment of the present invention.

[FIG. 2] (1), (2), (3), (4), (5), and (6) are vertical cross-sectional views showing the procedure for concentrating a sample using the device of FIG. 1, respectively. .

FIG. 3 shows a known closed type ultrafiltration sample concentrator,
(A) and (B) are vertical sectional views for a single sample and a large number of samples, respectively.

[Explanation of symbols]

 1 Concentrator body 2 Ultrafiltration membrane 3 Connection tube 4 Piston 5 Vacuuming port 6 Sample injection / exhaust port 7 Septum seal 8 Manual pump 9 Culture chamber 10 Sample cassette 11 Air bleeding syringe 02 Concentration sample 04 Connection tube 05 Gas liquid Separator

Claims (1)

[Claims] 1. A closed-type concentrating instrument body capable of containing a concentration sample, an ultrafiltration membrane disposed inside the concentrating instrument body, and a fluid from the ultrafiltration membrane to the outside of the concentrating instrument body. A closed type ultrafiltration type sample concentrator having a densely extending tube, a gas-liquid separator inserted in the tube, and a vacuuming means connected to the tip of the tube, wherein the central axis of the concentrating instrument main body An ultrafiltration membrane that can be moved along, and a septum-sealed vacuum suction port attached to the tip and side surfaces of the concentrating instrument body, respectively.
A hermetically sealed suction type ultrafiltration type sample concentrating device comprising a sample pouring / discharging port and a manual pump connected to the tip of the tube.