JPH06128138A - Cosmetic - Google Patents

Abstract

(57) [Summary] [Structure] A makeup containing natural melanin that is easily soluble in alkali and one or more components selected from a body moisturizing component, a plant extract, a tissue extract component, an antioxidant and a whitening agent. Fee. [Effect] It is highly safe for the skin and is excellent in preventing and improving rough skin.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to cosmetics which are excellent in preventing and improving skin roughness and have high skin safety.

[0002]

2. Description of the Related Art Conventionally, in order to prevent and improve rough skin, various biological moisturizing ingredients, botanical extracts, tissue extracts, antioxidants and other medicinal ingredients, and whitening skin Cosmetics containing a whitening agent having an effect are used. However, these cosmetics were not able to obtain a sufficient effect.

On the other hand, for human skin, it has been reported that excessive ultraviolet rays may cause acute inflammation of the skin, or may contribute to premature aging of the skin or skin cancer due to long-term exposure, which causes rough skin. ing. Therefore, cosmetics containing an ultraviolet blocking agent are used for the purpose of absorbing or scattering ultraviolet rays. Conventionally used ultraviolet blocking agents are used for cosmetics due to skin safety and an increase in the blending amount. There was a problem in terms of the effect on the product system, such as solubility in water and stability.

Therefore, there has been a demand for a cosmetic which is excellent in the effect of preventing and improving rough skin and has high stability to the skin.

[0005]

[Means for Solving the Problems] Therefore, as a result of various investigations by the present inventors in order to search for a UV absorber from among highly safe natural ingredients, the alkali-soluble natural melanin shows excellent UV absorption ability. In addition, a combination of this and one or more selected from specific biological moisturizing ingredients, plant extracts, tissue extract ingredients, antioxidants and whitening agents that have been used for preventing and improving rough skin. It was found that a cosmetic composition having high safety for the skin and synergistically excellent in the effect of preventing and improving skin roughness can be obtained when used, and the present invention has been completed.

That is, the present invention provides a natural melanin which is easily soluble in alkali and one or more components selected from the following (a) to (e): (a) Hyaluronic acid and its salts, collagen and elastin One or more kinds of biological moisturizing ingredients selected from (b) one or more kinds of plant extracts selected from sardine, psyllium, lily, touki, astragalus, arnica, chamomile, birch and carrot (c) deoxyribo One or more tissue extract components selected from nucleic acid, yeast extract, bovine blood deproteinizing extract, lactic acid bacterium fermentation metabolite and beef thymus extract (d) Vitamin E and its derivative, vitamin B ₂ and its derivative And one or two selected from phospholipids
One or more antioxidants (e) ascorbic acid and its derivatives, kojic acid and its derivatives, bovine placenta extract, hydroquinone derivative,
It is intended to provide a cosmetic containing one or more kinds of whitening agents selected from glutathione, cystine derivatives, sowaku extract and licorice extract.

The natural melanin which is easily dissolved in the alkali used in the present invention is not limited in its origin as long as it is easily dissolved in a weak alkaline aqueous solution, for example, an alkaline aqueous solution having a pH of 10 or less. preferable. Of these, alkali easily soluble natural melanin derived from microorganisms is particularly preferable. As a microorganism that produces the natural melanin, Aureobasidium (Aureobasi)
genus Aspergillus
s) genus, Streptomyces
s) Microorganisms belonging to the genus. Melanin derived from these microorganisms is, for example, FMS Microbiology Letters (FEMSMicrob).
iology Letters), 9 , 237-240.
(1980), Transactions of the British Mythological Society (Trans.
Br. Mycol. Soc. ), 70 (3), 453-
455 (1978), Molecular and General Genetics (Mol. Gen. Gene).
t. ), 200 , 422-432 (1985) and the like.

The ultraviolet absorption spectrum and safety test results of melanin derived from a microorganism, which is a naturally soluble melanin in alkali, are shown below.

(1) Ultraviolet Absorption Spectra Molecular and General Genetics (Mol. Gen. Genet.) 200 , 422-4
32 (1985), and added to 50 g of 6N hydrochloric acid to 1.4 g of the crude microorganism melanin produced by the method of 110.
Hydrolysis was performed by heating at ℃ for 48 hours. To this, 100 ml of 6N sodium hydroxide was added and dissolved. The solution was filtered through glass fiber filter paper, and the filtrate was diluted with 6N hydrochloric acid (200N).
ml was added. The precipitate was separated by sedimentation, 1 liter of water was added to the precipitate, and sedimented again. By freeze-drying the precipitate, 1 g of purified melanin was obtained. An ultraviolet absorption spectrum of a 0.01% aqueous solution of the purified melanin (hereinafter abbreviated as “microbial melanin A”) and synthetic melanin (manufactured by Sigma) is shown in FIG. From Figure 1, the microbial melanin
It has a superior ultraviolet absorption effect compared to synthetic melanin, and has the effect of protecting hair damage due to ultraviolet rays.

(2) Safety test Regarding the safety of microbial melanin A, a human 48 hr closed patch test [Nippon Kaihatsu, 80 (5), 301-3]
14 (1970)], single oral administration toxicity test [Ministry of Health, Labor and Welfare Drug Examination No. 118 (February 15, 1984), Guidelines for toxicity tests necessary for drug manufacturing (import) approval application (Part 1)] And skin photosensitization test (Seijin skin, 4
Volume 2, No. 5). As a result, as shown in Table 1,
It was found that the microbial melanin is highly safe not only when it is orally administered but also when it is applied to the skin.

[0011]

[Table 1]

From these results, it is understood that the easily soluble alkali natural melanin has an effect of preventing rough skin due to an excellent ultraviolet absorbing action and is highly safe. Furthermore, the easily soluble alkali microbial melanin has been found to have antioxidant ability, and also has the effect of preventing skin aging by active oxygen and the like.

In the cosmetic composition of the present invention, the easily soluble alkali natural melanin is contained in an amount of 0.01 to 20% by weight (hereinafter simply referred to as%), preferably 0.1 to 5% in the total composition. .

On the other hand, the biological moisturizing component (a) is one or more selected from hyaluronic acid and its salts, collagen and elastin, all of which are known as skin constituents. Is. The origin of these biological moisturizing components is not particularly limited, and may be animal-derived, microbial-derived, or synthetic. Further, the extraction method and the purification treatment method in the case of natural origin are not particularly limited. Of the biological moisturizing ingredients, hyaluronic acid and salts thereof include, for example, umbilical cords, chicken gooseberry, animal skin, ocular glass bands, and molecular weights of 100,000 to 3,000,000 obtained by subjecting cultures of microorganisms to extraction treatment. Examples thereof include sodium, potassium, basic amino acid salts, and the like, and examples of collagen include soluble collagen obtained by subjecting skin tissue of cattle or pigs to an extraction treatment, or by hydrolyzing it, or Examples thereof include those that have been further subjected to treatment such as succinylation. Further, examples of elastin include soluble elastin having a molecular weight of 10,000 to 5,000,000 obtained by hydrolyzing elastin purified from bovine nuchal ligament.

The component (b), the plant extract, is one or more selected from sorghum, psyllium, lily, touki, astragalus, arnica, chamomile, birch and carrot. These plant extracts can be obtained by subjecting each plant to extraction and purification treatment according to a conventional method, and the method is not particularly limited. For example, leaves, stems, roots and the like of each plant are used as a solvent, primary alcohols such as water, methyl alcohol and ethyl alcohol, liquid polyhydric alcohols such as propylene glycol and 1,3-butylene glycol, and lower grades such as ethyl acetate. Examples thereof include a method of extraction using a hydrocarbon such as alkyl ester, benzene, hexane, etc., ethyl ether, acetone, etc. These solvents can be used alone or in combination of two or more kinds. Among these, particularly preferable solvents include aqueous solutions of water-miscible organic solvents, particularly ethyl alcohol, methyl alcohol, and acetone.

The tissue extract component of component (c) is one selected from deoxyribonucleic acid (hereinafter referred to as "DNA"), yeast extract, bovine blood deproteinizing extract, lactic acid bacterium fermentation metabolite, and beef thymus extract. Two or more. As the DNA, for example, DNA-S (manufactured by Organic Synthetic Chemicals) or the like can be used. The yeast extract is, for example, yeast itself such as baker's yeast or brewer's yeast, or an aqueous extract obtained by treating the dried powder thereof by physical or biochemical means, amino acids, peptides, and organic compounds. It contains acid, nucleic acid and the like. As a commercial product,
"Yeast extract A-33" (Asahi Brewery Co., Ltd.) etc. can be used.

The bovine blood deproteinized extract is obtained by subjecting the blood of an adult or a calf to an appropriate treatment and then removing the protein. Although the method for producing the same is not particularly limited, for example, the blood used as a raw material may be collected from a calf that has activated the reticuloendothelial system, or may be fresh blood of a slaughtered cow. Bovine blood is processed by heat treatment, enzymatic decomposition, electrolysis, etc., in addition to freezing. The protein can be removed by an ultrafiltration method, a precipitation method or the like, and is not particularly limited as long as it is a commonly used method. Examples of the commercially available bovine blood deproteinization extract include stimulusel (manufactured by Pentafarm) and solcoceryl (manufactured by Toryo Yakuhin), which can be preferably used. In the present invention, these bovine blood deproteinizing extracts are the extracts as they are,
Alternatively, it can be used as a solid.

The lactic acid bacterium fermentation metabolite is produced, for example, by inoculating lactic acid bacteria into a culture medium containing animal milk such as milk as a main component to carry out lactic acid fermentation, and separating whey from the resulting culture. As such, Lactobacillus acidophilus, Streptococcus thermophilus and the like can be used. As a commercially available product, Spyron-L (manufactured by Sansho Pharmaceutical Co., Ltd.) or the like can be used. Beef thymus extract is
A hormone-free water-soluble extract extracted from calf thymus and deproteinized, which contains various amino acids and low molecular weight proteins. As a commercially available product, CYMAPEPTIDE (manufactured by Pentafarm) or the like can be used.

The antioxidant as the component (d) is one or more selected from vitamin E and its derivatives, vitamin B ₂ and its derivatives, and phospholipids.
As the vitamin E derivative, for example, vitamin E acetate (manufactured by Eisai Co., Ltd.) or the like can be used. As the vitamin B ₂ derivative, for example, FAD (manufactured by Kyowa Hakko Co., Ltd.) or the like can be used. Examples of the phospholipids include natural phospholipids extracted from microorganisms such as animals and plants and Escherichia coli, hydrogenated products thereof, and synthetic phospholipids. For example, Nikkorresinol S-10 (manufactured by Nikko Chemicals Co., Ltd.) and the like are used. be able to.

The whitening agent (e) is one or two selected from ascorbic acid and its derivative, kojic acid and its salt, bovine placenta extract, hydroquinone derivative, glutathione, cystine derivative, sowaku extract and licorice extract. More than a seed. As derivatives of ascorbic acid, L-ascorbic acid monostearate,
Examples thereof include oil-soluble ascorbic acid derivatives such as L-ascorbic acid dipalmitate and water-soluble ascorbic acid derivatives such as L-ascorbic acid phosphoric acid ester. Examples thereof include Nikkor VC-PMG (manufactured by Nikko Chemicals Co., Ltd.) and Nikkor VC-SS ( Nikko Chemicals Co., Ltd.) can be used. Examples of derivatives of kojic acid include monoesters of kojic acid monobutyrate and kojic acid monocaprate, and diesters of kojic acid dibutyrate and kojic acid distearate. As the bovine placenta extract, for example, a water-soluble placenta extract (manufactured by Nichirei Co.) or the like can be used. Examples of the hydroquinone derivative include a condensate of hydroquinone and sugar, and a condensate of alkylhydroquinone and sugar in which one alkyl group having 1 to 4 carbon atoms is introduced into hydroquinone, and examples thereof include arbutin. Examples of the cystine derivative include N, N′-diacetylcystine dimethyl, and the like. For example, CIP
(Made by Katsura Trading Co., Ltd.) can be used. As the sowakuhi extract, sokuhaku extract (manufactured by Maruzen Pharmaceutical Co., Ltd.) and the like can be used. As the licorice extract, a licorice extract extract (made by Maruzen Pharmaceutical Co., Ltd.) and the like can be used.

In the present invention, if one or more selected from the components (a) to (e) are used in combination, the effect of preventing and improving skin roughness becomes synergistically excellent.
When the whitening agent of the component (e) is used in combination, a particularly excellent effect of preventing skin blackening and an effect of improving blackening can be obtained.

The components (a) to (e) may be used either individually or in combination of two or more, and are added in an amount of 0.0001 to 5%, preferably 0.001 to 3% in the total composition. preferable.

In the cosmetic of the present invention, in addition to the above-mentioned essential components, components used in ordinary cosmetics, such as oils and fats,
Hydrocarbons, waxes, fatty acids, synthetic esters, higher alcohols, alcohols (lower alcohols, polyhydric alcohols), surfactants, thickeners, preservatives, fragrances, various beauty ingredients, pigments, purified water And the like can be appropriately blended within a range that does not impair the effects of the present invention.

Examples of the oils and fats include jojoba oil, castor oil, olive oil, soybean oil, coconut oil, palm oil, cocoa oil, mink oil, turtle oil, fatty acid diethanolamide and the like. Examples of the hydrocarbons include liquid paraffin, petrolatum, microcrystalline wax, squalane and the like. As waxes, beeswax, lanolin,
Examples include carnauba wax and candelilla wax. Examples of the fatty acids include myristic acid, palmitic acid, stearic acid, oleic acid and isostearic acid. Synthetic esters include isopropyl myristate, isopropyl palmitate, butyl oleate, myristyl myristate, octyldecyl myristate,
Propylene glycol monostearate, myristyl lactate, isostearyl malate, glyceryl monostearate, distearyl dimethyl ammonium chloride and the like can be mentioned. Examples of higher alcohols include lauryl alcohol, cetanol, stearyl alcohol, and euler alcohol. Examples of alcohols (lower alcohols and polyhydric alcohols) include ethanol, glycerin, 1,3-butylene glycol and propylene glycol.

As the surface active agent, polyoxyethylene hydrogenated castor oil, sodium lauryl sulfate, polyoxyethylene glyceryl isostearate pyroglutamate,
Sodium alkylbenzenesulfonate, polyoxyethylene (10) stearyl ether, dialkylsulfosuccinic acid, cetylpyridinium bromide, n-octadecyltrimethylammonium chloride, monoalkylphosphoric acid, N-acylglutamic acid, sucrose fatty acid ester, polyoxyethylene (20) Examples thereof include sorbitan monostearate, sodium polyoxyethylene lauryl ether sulfate, and polyoxyethylene reduced lanolin.

Examples of the thickener include carboxyvinyl polymer, methylpolysiloxane, dextran, carboxymethylcellulose, carrageenan and hydroxymethylcellulose. As antiseptics, benzoic acid, salicylic acid, dehydroacetic acid or salts thereof,
Phenols such as paraoxybenzoic acid ester, 2,
4,4'-trichloro-2'-hydroxydiphenyl ether, 3-trifluoromethyl-4,4'-dichlorocarbanilide and the like can be mentioned.

Various cosmetic ingredients include vitamin A, allantoin, sage extract, peony extract, seaweed extract, avocado oil, lactic acid, linoleic acid and the like.

The cosmetic composition of the present invention can be produced according to an ordinary method by mixing natural melanin which is easily soluble in alkali and one or more kinds selected from the components (a) to (e). Further, the form of the cosmetic is not particularly limited,
For example, it can be an emulsion, a cream, a lotion, a pack, a dispersion, a cleaning agent and the like.

[0029]

The present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

Examples 1 to 5 Creams: Creams having the compositions shown in Table 5 were produced and evaluated for their skin roughening preventive effect, skin roughening improving effect, blackening preventing effect and blackening improving effect. The results are shown in Tables 6 to 7.

(Production Method) A. The components (1) to (7) and (12) are heated and mixed,
Keep at 70 ° C. B. Ingredients (8)-(11), (13)-(16) and (18) are heat mixed and maintained at 70 ° C. C. The above A is added to B, mixed, and uniformly emulsified. D. After cooling the above C, (17) is added and uniformly mixed to obtain a cream.

(Evaluation Method) (1) Skin Roughness Preventing Effect 50 males aged 22 to 32 were used as a panel, and after applying the test cream, the degree of erythema and the skin condition when irradiated with ultraviolet rays for 30 minutes by a solar simulator were measured. Observed and scored according to the following criteria. The results are shown as the number of people in each score.

[0033]

[Table 2] [Score] [Status] 4 No erythema is observed. 3 Erythema is slightly observed. 2 Erythema is observed. 1 Erythema was clearly observed, and the skin surface was slightly sore.

(2) Skin Roughening Improvement Effect A panel of 60 women aged 23 to 30 was used to observe the skin condition on the day before skiing with a microscope camera, and the score was calculated according to the following criteria. The skin condition score was also obtained one day after returning from skiing. Further, thereafter, the test cream was applied twice daily in the morning and the evening for seven days, and the skin condition score was obtained after 3, 5 and 7 days after skiing as described above. The results are shown as the average score.

[0035]

[Table 3] [Score] [State] 1 The skin groove of the skin is unclear and peeling of the keratin is observed. 2 The skin groove on the skin is slightly blurred or unidirectional. 3 Skin groove is recognized, but shallow or unidirectional. 4 Skin crevices are observed or are slightly mesh-like. 5 Skin groove is clearly visible or has a clean mesh.

(3) Skin Blackening Preventive Effect and Blackening Improving Effect 20 specialist panels used the test cream, and the skin blackening preventing effect and the blackening improving effect were evaluated according to the following criteria. It was judged by the average score.

[0037]

[Table 4] [Evaluation Criteria] 5; Good 4; Slightly Good 3; Normal 2; Slightly Bad 1; Bad Average score less than 2.5

[0038]

[Table 5]

[0039]

[Table 6]

[0040]

[Table 7]

As is clear from the results shown in Tables 6 to 7, the cosmetics of the present invention show an excellent effect of preventing rough skin and an effect of improving rough skin. Particularly, those containing a whitening agent have a blackening preventive effect and a blackening effect. The improvement effect was excellent.

Examples 6 to 8 Emulsion: An emulsion having the composition shown in Table 8 was produced. The obtained emulsion was highly safe and had excellent effects of preventing and improving rough skin.

(Production Method) A. (1) to (7) are mixed by heating and kept at 70 ° C. B. (8) to (14) and (16) are heated and mixed,
Keep at 0 ° C. C. A is added to the above B and mixed to uniformly emulsify. D. After cooling the above C, (15) is added and uniformly mixed to obtain an emulsion.

[0044]

[Table 8]

Examples 7-9 Lotion: Lotion with the composition shown in Table 9 was produced. The obtained lotion was highly safe and had excellent effects of preventing and improving rough skin.

(Production Method) A. (1), (5) and (11) are mixed and dissolved. B. (2) to (4), (6) to (10) and (12)
And dissolve. C. A is added to the above B, and after mixing, lotion is obtained.

[0047]

[Table 9]

[0048]

The cosmetics of the present invention are highly safe to the skin and have excellent effects of preventing and improving rough skin.

[Brief description of drawings]

FIG. 1 is a diagram showing ultraviolet absorption spectra of microbial melanin A and synthetic melanin.

Continuation of front page (51) Int.Cl. ⁵ Identification number Office reference number FI technical display location A61K 7/00 D 7252-4C W 7252-4C 35/78 C 7167-4C

Claims (1)

[Claims] 1. A naturally soluble alkaline melanin and one or more components selected from the following (a) to (e): (a) Hyaluronic acid and salts thereof, collagen and elastin 1 Or two or more kinds of biological moisturizing components (b) one or more kinds of plant extracts selected from sorghum, psyllium, lily, touki, astragalus, arnica, chamomile, birch and carrot (c) deoxyribonucleic acid, yeast extract Liquid, bovine blood deproteinization extract, lactic acid bacterium fermentation metabolite and one or more tissue extract components selected from beef thymus extract (d) Vitamin E and its derivatives, vitamin B ₂ and its derivatives and phospholipids 1 or 2 chosen
One or more antioxidants (e) ascorbic acid and its derivatives, kojic acid and its derivatives, bovine placenta extract, hydroquinone derivative,
A cosmetic containing one or more whitening agents selected from glutathione, cystine derivatives, sour apricot extract and licorice extract.