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JPH04500004A - 真核細胞におけるポリシストロン性メッセージの高率翻訳 - Google Patents

真核細胞におけるポリシストロン性メッセージの高率翻訳

Info

Publication number
JPH04500004A
JPH04500004A JP1508441A JP50844189A JPH04500004A JP H04500004 A JPH04500004 A JP H04500004A JP 1508441 A JP1508441 A JP 1508441A JP 50844189 A JP50844189 A JP 50844189A JP H04500004 A JPH04500004 A JP H04500004A
Authority
JP
Japan
Prior art keywords
leader
protein
fragment
expression
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1508441A
Other languages
English (en)
Japanese (ja)
Inventor
バークナー,キャスリーン エル.
Original Assignee
ザイモジェネティクス,インコーポレイティド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ザイモジェネティクス,インコーポレイティド filed Critical ザイモジェネティクス,インコーポレイティド
Publication of JPH04500004A publication Critical patent/JPH04500004A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/44Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP1508441A 1988-07-29 1989-07-26 真核細胞におけるポリシストロン性メッセージの高率翻訳 Pending JPH04500004A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22617388A 1988-07-29 1988-07-29
US226,173 1988-07-29

Publications (1)

Publication Number Publication Date
JPH04500004A true JPH04500004A (ja) 1992-01-09

Family

ID=22847870

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1508441A Pending JPH04500004A (ja) 1988-07-29 1989-07-26 真核細胞におけるポリシストロン性メッセージの高率翻訳

Country Status (4)

Country Link
EP (1) EP0426744A1 (fr)
JP (1) JPH04500004A (fr)
AU (1) AU4036389A (fr)
WO (1) WO1990001550A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008307056A (ja) * 1997-03-12 2008-12-25 Virogenetics Corp 発現の向上したベクター並びにその製造および使用方法
WO2013161958A1 (fr) 2012-04-27 2013-10-31 日本ケミカルリサーチ株式会社 Nouveau vecteur d'expression

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69232438T2 (de) * 1991-08-07 2002-10-10 W. French Anderson Interne ribosom eintrittsstellen enthaltene retrovirale vektoren
US5648235A (en) * 1992-07-03 1997-07-15 Q.B.I. Enterprises Ltd. Method and means for the production of gene products, novel recombinant DNA vectors therefor and kits employing them
DE4228458A1 (de) * 1992-08-27 1994-06-01 Beiersdorf Ag Multicistronische Expressionseinheiten und deren Verwendung
DE4228457A1 (de) * 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
AU6230394A (en) * 1993-01-20 1994-11-21 Biotransplant Incorporated Retroviral vectors capable of expressing multimeric proteins from multiple translational initiation sites
AU780164B2 (en) * 1994-09-13 2005-03-03 Transgene S.A. Novel implant and novel vector for the treatment of acquired diseases
FR2724320B1 (fr) 1994-09-13 1996-12-20 Transgene Sa Nouvel implant pour le traitement des maladies acquises
US5518042A (en) * 1994-09-16 1996-05-21 Huyck Licensco, Inc. Papermaker's forming fabric with additional cross machine direction locator and fiber supporting yarns
AT403167B (de) * 1994-11-14 1997-11-25 Immuno Ag Selektion und expression von fremdproteinen mittels eines selektions-amplifikations-systems
IL112872A0 (en) * 1995-03-03 1995-06-29 Q B I Enterprises Ltd Novel recombinant dna vectors and kits employing them
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
US7037715B2 (en) 1999-10-15 2006-05-02 The Mclean Hospital Multimerized DBH enhancer domains
CA2386289A1 (fr) * 1999-10-15 2001-04-26 The Mclean Hospital Corporation Domaines multimerises d'activateurs dbh
AU2002348382A1 (en) 2001-10-19 2003-04-28 The Mclean Hospital Corporation Multimerized enhancer domains for cell-specific expression

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713339A (en) * 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
EP0154576A1 (fr) * 1984-02-06 1985-09-11 INTERFERON SCIENCES, INC a Delaware Corporation Séquences d'acides nucléiques hybrides ayant des gènes disposés en tandem
JPH0630590B2 (ja) * 1985-08-30 1994-04-27 株式会社ヤクルト本社 複合プラスミドベクタ−
ATE72837T1 (de) * 1985-11-05 1992-03-15 Genetics Inst Vielfach amplifizierbare vektoren fuer hohe expression exogener dns.
WO1988005466A2 (fr) * 1987-01-15 1988-07-28 Codon Vecteurs d'expression eukaryotique de genes en tandem

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008307056A (ja) * 1997-03-12 2008-12-25 Virogenetics Corp 発現の向上したベクター並びにその製造および使用方法
WO2013161958A1 (fr) 2012-04-27 2013-10-31 日本ケミカルリサーチ株式会社 Nouveau vecteur d'expression

Also Published As

Publication number Publication date
WO1990001550A1 (fr) 1990-02-22
AU4036389A (en) 1990-03-05
EP0426744A1 (fr) 1991-05-15

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