JPH04346908A - Cosmetic containing partially degraded product of guar gum - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to cosmetics having a moisturizing effect. More specifically, the present invention relates to a cosmetic containing a partially decomposed guar gum having a viscosity of 3 to 20 cP at 25° C. when made into a 1% (W/W) aqueous solution.

0002

BACKGROUND OF THE INVENTION Conventionally, various polymeric substances have been blended into cosmetics for the purpose of imparting moisture and moisturizing properties to the skin. Polymer substances themselves have a strong ability to retain moisture,
It works to retain an appropriate amount of moisture on the skin and prevent it from drying out.

[0003] Examples of polymeric substances used for this purpose include plant polysaccharides such as locust bean gum, quince seed, and carrageenan, animal polymeric substances such as hyaluronic acid, and synthetic polymeric substances. Each has different characteristics in terms of moisturizing power and feel on the skin.

[0004] Among these, guar gum is cheaper and more economical than locust bean gum and hyaluronic acid.

[0005] Guar gum is a natural vegetable polysaccharide whose constituent sugars are galactose and mannose, and is a substance that exhibits extremely high viscosity even in a small amount in an aqueous solution. However, when applying guar gum to cosmetics, the viscosity is too high, and as a result, it is difficult to remove the precipitate formed in the aqueous solution due to components other than the polysaccharide contained in small amounts in guar gum, and the transparency is low. Therefore, the amount added was limited to a very small amount. In other words, when it comes to adding guar gum to cosmetics, conventionally it has only been added in a very small amount as a stabilizer or thickener, and it has not been added in an effective amount to bring out the moisturizing and film-forming effects of guar gum. It was possible.

[0006]

[Problems to be Solved by the Invention] In view of these circumstances, the present invention aims to reduce the viscosity and make guar gum transparent by partially decomposing it while retaining its moisturizing properties and film-forming effects, and as a result, adds an effective amount to cosmetics. The purpose of this invention is to provide cosmetics with excellent moisturizing and film-forming effects.

[0007]

[Means for Solving the Problems] That is, the present invention provides 1%
(W/W) 3 to 20 cP at 25℃ when made into an aqueous solution
The present invention relates to a cosmetic containing a partially decomposed product of guar gum having a viscosity of .

[0008]

[Example] As a result of intensive research to achieve the above object, the present inventors have found that guar gum can be added to cosmetics without losing its moisturizing effect and film-forming effect, and with a low viscosity. The present invention was completed based on the discovery that the degree of partial decomposition of guar gum, its partial decomposition method, and cosmetics containing the partial decomposition product of guar gum have excellent moisturizing effects and film-forming effects, are less sticky, and are gentle on the skin. I ended up doing it.

Next, the cosmetic of the present invention will be explained.

[0010] The above-mentioned 1% contained in the cosmetic of the present invention.
(W/W) When made into an aqueous solution, the viscosity is 3 to 2 at 25℃.
0cP guar gum partial decomposition product is usually about 5000~2
It has an average molecular weight of 0,000 and a degree of polymerization of about 10 to 40, and is a yellowish-white powder that easily dissolves in water.

[0011] When applying a partially decomposed product of guar gum to cosmetics, if the viscosity is 20 cP or more, the moisturizing effect and film-forming effect will be maintained, but the high viscosity and low transparency will cause cosmetic problems. It is difficult to add an effective amount to cosmetics, and if the viscosity is less than 3 cP, the moisturizing effect and film-forming effect of cosmetics will decrease.

Examples of methods for partially decomposing guar gum to the above-mentioned viscosity include, but are not limited to, hydrolysis with acid or alkali, treatment with enzymes, and biodegradation treatment with microorganisms.

However, biodegradation treatment using microorganisms is preferred from the viewpoint of ease of adjusting the degree of decomposition, ease of purification after treatment, and cost.

[0014] The acid used for acid hydrolysis is not particularly limited, and examples thereof include hydrochloric acid, sulfuric acid, and nitric acid.

[0015] The alkali used in alkaline hydrolysis is not particularly limited, and examples thereof include sodium hydroxide and potassium hydroxide.

[0016] The enzyme used in the enzymatic treatment may be any enzyme as long as it can decompose guar gum, and examples include galactomannase, mannase, and galactosidase.

The microorganism used in the microbial biodegradation treatment may be any microorganism as long as it can decompose guar gum, but bacteria belonging to the genus Bacillus are preferred, such as Bacillus subtilis.
) (IFO 13719, IFO 3215), Bacillus circulans
ulans) (IFO 13294), Bacillus
Bacillus amyl
oliquefaciens) (IFO 14141
) and Bacillus polymyxa (Bacillus
polymyxa) (IFO 3020). These have been deposited and can be easily obtained. Biodegradation treatment uses guar gum from 0.1 to 0.
．． Bacteria pre-cultured in a medium suitable for the bacteria containing 2% (W/V) guar gum and 0.5-2.0% (W/V)
The culture is carried out by inoculating and culturing the same medium as above except for the above. The culture temperature varies depending on the bacteria used, but it is
The temperature is preferably 35°C, and the culture time is preferably 6 to 48 hours. If the culture time is less than 6 hours, the viscosity reduction is insufficient, and if it is more than 48 hours, fermentation odor may occur due to metabolism.

The content of the guar gum partially decomposed product in the cosmetics is preferably 0.0% in terms of solid content (hereinafter, all contents of guar gum partially decomposed products are referred to as solid content).
01-2.0% (W/W), more preferably 0.01-2.0% (W/W).
05 to 1.0% (W/W). Content is 0.0
If it is less than 1% (W/W), the moisturizing effect and film-forming effect of the partially decomposed guar gum product will not be exhibited.
If the amount is more than 2.0% (W/W), the stickiness will be strong and the feel will be poor.

Furthermore, in addition to the partially decomposed product of guar gum, the cosmetics of the present invention also contain, for example, fragrances commonly used in cosmetics, oils and fats, surfactants, humectants, and cosmetics.
Various cosmetic ingredients such as H adjusters, thickeners, preservatives, antioxidants, ultraviolet absorbers, pigments, detergents, and emulsifiers are appropriately blended.

The form of the cosmetic of the present invention is arbitrary and is not limited, but since it has excellent moisturizing effect and film-forming effect, it may be, for example, cream, milk lotion,
Examples include lotions, packs, serums, hair creams, styling gels, hair liquids, cream foundations, and liquid foundations.

Next, the cosmetic composition of the present invention will be explained in more detail based on Examples, but the present invention is not limited to these Examples.

Example 1 (Partial decomposition of guar gum by a microorganism belonging to the genus Bacillus) First, a microorganism belonging to the genus Bacillus, Bacillus circulans No. 37 (hereinafter referred to as “K-1”) (
(identified in Reference Example 1 shown later), 0.2% (W/V)
Guar gum-containing synthetic medium (guar gum (May Pro Guar G 200/50, manufactured by Sansho Co., Ltd.): 2.0 g, K
2 HPO4: 3.0g, KH2PO4: 0.3
g, (NH4)2SO4: 1.0g, MgSO4
・7H2O: 0.2g, NaCl: 0.1g, and CaCl2.2H2O: 0.02g dissolved in 1000ml of distilled water) were inoculated and precultured at 30°C for 48 hours. Add this culture solution to 1% (W/V) guar gum-containing synthetic medium (guar gum: 10.0 g, other salts are the same as the 0.2% guar gum-containing synthetic medium).
After adding 0.00 volume and culturing at 30°C for 18 hours, sterilization was performed in an autoclave to stop the biodegradation reaction. Insoluble matter is removed from the culture solution obtained in this way by centrifugation or filtration, an excess amount of alcohol is added to the resulting clear solution, the precipitate is filtered and dried, and guar gum is partially decomposed from 1 liter of the culture medium. I got 9.0g of food.

The properties of the obtained partial decomposition product are shown below.

(1) The viscosity of the 1% (W/W) aqueous solution was measured using a B-type viscometer and was found to be 5 cP (25° C.). As a control, 1% (W/W) of raw guar gum
When the viscosity of the aqueous solution was measured using a B-type viscometer, it was found to be 330.
It was 0 cP (25°C).

(2) The molecular weight was measured by gel filtration chromatography, and the results were as follows.

Number average molecular weight 6,070 (1,940,000 for raw guar gum) Weight average molecular weight 336,000 (4,900,000 for raw guar gum) (3) The galactose:mannose ratio was changed by acid hydrolysis treatment (5% sulfuric acid, 100°C, 2.5 hours) and then measured by high performance liquid chromatography, the ratio was 1:1.6.
Met.

[0027] As a control, the raw material guar gum was measured and found to be 1:1.7.

Reference Example 1 (Identification of the strain used in Example 1) Bacterium K used in Example 1
-1, we conducted identification tests on various items. The obtained data were compared with literature values and it was presumed to be Bacillus circulans.

Next, a confirmation test for the same items was conducted using a standard bacterium of Bacillus circulans (IFO 13627).

The results are shown in Table 1.

[0031]

[Table 1]

Although no spore formation was observed in K-1, all properties other than spore formation were consistent with the standard bacteria, and the strength of the reaction was the same in each property test. From this result, K-1 was presumed to be a strain of Bacillus circulans that is the same as the standard strain, but lacking spore-forming ability.

Example 2 (Guar gum decomposition ability test of bacteria) 10 species of bacteria of the genus Bacillus including Bacillus circulans and 15 bacteria of other genera
Regarding the seeds, the decomposition ability of guar gum was investigated in the same manner as in Example 1.

[0034] Each bacterium was grown in BPY medium (3.0 g of meat extract, 3.0 g of yeast extract, 10 g of polypeptone).
Dissolve 0g in 1000ml of distilled water and adjust the pH to 7.4.
(adjusted to ) and precultured at 30°C for 24 hours. Add 1/100 volume of this culture solution to a synthetic medium containing 1% (W/V) guar gum (described above) and store at 30℃ for up to 5 hours.
After culturing for days, bacteria whose viscosity decreased to 20 cp or less within 5 days were determined to have decomposition ability.

The results are shown in Table 2 along with the accession numbers of the bacteria.

[0036]

[Table 2]

Example 3 (Relationship between culture time and viscosity of partially degraded guar gum) K
-1 was used to investigate the relationship between culture time and viscosity of partially degraded guar gum.

[0038] The medium pre-cultured in the same manner as in Example 1 was added to a synthetic medium containing 1.0% (W/V) guar gum.
Add 100 volumes and leave to stand at 30°C, 0, 6, 10, 2
Cultures were carried out for 4 and 51 hours.

1% (W
/W) Measure the viscosity (25°C) of the aqueous solution using a B-type viscometer, 60r
Measured in pm.

The results are shown in Table 3.

[0041]

[Table 3]

FIG. 1 shows a graph in which the viscosity is plotted on a logarithmic scale on the vertical axis and the culture time is plotted on the horizontal axis.

From FIG. 1, the culture time for decomposition is 6~
48 hours was found to be preferable.

Example 4 (Comparison of decomposition treatment methods) Guar gum (Maypro Guar)
CSA 200/50, manufactured by Sansho Co., Ltd.) (viscosity: 1.
0% (W/W) aqueous solution (2500 cP (25°C)) was decomposed under various conditions, and the viscosity of the partially decomposed product 1% (W/W) aqueous solution was compared. The results are shown in Table 4.

[0045]

[Table 4]

Formulation Example 1 (Cream) As a cosmetic of the present invention, a cream was prepared using the partially decomposed guar gum obtained in the same manner as in Example 1 according to the following formulation.

(Ingredients)

(Parts by weight) 1 Polyoxyethylene sorbitan monostearic acid
1.0 Ester (20E.O.)
2 Polyoxyethylene sorbitan tetraoleic acid 1.5
Ester (60E.O.) 3 Glycerin monostearate
1.5 4 Beeswax

2.0
5 Paraffin

2.0 6 Stearic acid

3.0 7 Squalane
6.0
8 Olive oil

9.0 9 Methyl paraoxybenzoate
0.1 10 2.0
% (W/W) Guar gum partial decomposition product aqueous solution (viscosity =
16cP) 20.0 11 Fragrance

0.1
12 Purified water

53.8 (Note) "E.O." means the number of moles of ethylene oxide added (the same applies hereinafter).

Mix 1 to 9, heat and dissolve at about 80°C, add 10 and 12 heated to about 30°C to make a homogeneous solution, then add 11, and cool while stirring. It was creamy. The content of partially decomposed guar gum in the cosmetic was 0.4% (W/W).

Prescription Example 2 (Milk Lotion) As a cosmetic of the present invention, Example 1
A milk lotion was prepared using the following recipe using a partially decomposed guar gum product obtained in the same manner as above.

(Ingredients)

(Parts by weight) 1 Squalane

3.0
2 Tri(caprylic, capric) glycerin
5.5 3 Polyoxyethylene cetyl ether (25E.O.)
0.5 4 Methyl paraoxybenzoate
0.1 5 2
0% (W/W) carboxyvinyl polymer aqueous solution
1.0 6 Ethanol
10.
0 7 0.5% (W/W) Guar gum partial decomposition product aqueous solution (viscosity = 3 cP) 79.5
8 Sodium hydroxide

0.3 9 Fragrance

0.1 Mix 1 to 4, heat and dissolve at about 80°C, add 5 to 7 heated to about 80°C to make a homogeneous solution, then add 8 and 9, and stir. The mixture was cooled down and made into milk lotion. The content of partially decomposed guar gum in the cosmetic was 0.4% (W/W).

Formulation Example 3 (Lotion) As a cosmetic of the present invention, a lotion was prepared using the partially decomposed guar gum obtained in the same manner as in Example 1 according to the following formulation.

(Ingredients)

(Parts by weight) 1 Polyoxyethylene hydrogenated castor oil (30E.O.)
3.0 2 Ethanol

10.0 3
1,3-butylene glycol
2.0
4 1.0% (W/W) Guar gum partial decomposition product aqueous solution (viscosity = 5 cP) 80.0 5
Methyl paraoxybenzoate
0.1
6 Fragrance

0.1 7 Purified water

4.31
-7 were mixed and dissolved uniformly to prepare a lotion. The content of partially decomposed guar gum in cosmetics is 0.8% (W/W
) became.

Formulation Example 4 (Pack) As a cosmetic of the present invention, a pack was prepared using the partially decomposed guar gum obtained in the same manner as in Example 1 according to the following formulation.

(Ingredients)

(Parts by weight) 1 Polyvinyl alcohol
13.0 2 Ethanol
6.
0 3 Polyoxyethylene hydrogenated castor oil (3
0E. O. ) 0.5
4 Titanium oxide

7.0 5 Kaolin

5.0 6
5.0% (W/W) Guar gum partial decomposition product aqueous solution (viscosity = 105 cP) 8.0 7 Methyl paraoxybenzoate
0.1 8
fragrance

0.1 9 Purified water

60.3 Mix 1 to 9 to make a uniform paste and make a pack. The content of partially decomposed guar gum in cosmetics is 0.4
% (W/W).

Test Example 1 (Primary skin irritation test using rabbits) The backs of 6 female Japanese white improved rabbits (weight 2.0-3.0 kg) in one group were shaved with clippers. A 1% (W/W) aqueous solution of the guar gum partial decomposition product obtained in Reference Example 1 was applied in an amount of 0.1 g to a Tori band (regular size), and the adhesive was applied to the shaved area of each of the six animals for 24 hours. 24 hours and 72 hours after application, the irritation to the rabbit skin was evaluated according to the Draize criteria shown below.

[Evaluation criteria for skin reaction of primary skin irritation (
Draize Judgment Criteria) ■ Erythema and crust formation 0: No erythema 1: Very mild erythema (barely noticeable) 2: Obvious erythema 3: Moderate to strong erythema 4: Slight deep red erythema Eschar formation (injury reaches deep) Edema formation 0: No edema 1: Very mild edema (barely noticeable) 2: Obvious edema (clearly distinguishable from the surrounding area) 3: Moderate edema (raised by about 1 mm) 4: Strong edema (1 mm)
It becomes more exciting and spreads to the surrounding area)

[0057]

[Table 5]

As shown in Table 5, the results were negative in all cases, and no primary rabbit skin irritation was observed.

Test Example 2 (Lotion Monitor Test) Twenty-nine women aged 25 to 50 used the lotion obtained in Prescription Example 3 on their faces for one month and answered questions. . The results are shown in Tables 6 and 7.
8 and 9.

[0060]

[Table 6]

[0061]

[Table 7]

[0062]

[Table 8]

[0063]

[Table 9]

From the test results, it was found that the lotion, which is the cosmetic composition of the present invention, is completely non-irritating, has a pleasant texture, and has an excellent moisturizing effect.

[0065]

[Effects of the Invention] According to the present invention, guar gum, which has a moisturizing effect and a film-forming effect but cannot be added as a moisturizing agent to cosmetics due to its high viscosity and low transparency, can be easily used. By partially decomposing it using this method, it becomes possible to add it to cosmetics in an effective amount as a humectant.
A cosmetic having an excellent moisturizing effect can be obtained.

[Brief explanation of drawings]

FIG. 1 is a graph in which the culture time in Example 3 is plotted on the horizontal axis and the viscosity of the partially degraded guar gum is plotted on a logarithmic scale on the vertical axis.

Claims (3)

[Claims] 1. A cosmetic comprising a guar gum partially decomposed product having a viscosity of 3 to 20 cP at 25° C. when made into a 1% (W/W) aqueous solution. 2. The cosmetic according to claim 1, wherein the content of the partially decomposed guar gum product is 0.01 to 2.0% (W/W) in terms of solid content. 3. The cosmetic according to claim 1, wherein the guar gum partial decomposition product is obtained by biodegrading guar gum with a microorganism belonging to the genus Bacillus.