JP3652424B2 - Automatic analyzer and method - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an automatic analyzer suitable for an immunoassay based on a chemiluminescence method and a method thereof, and more particularly, an automatic analysis capable of automatically performing this kind of test consisting of complicated and various processing steps. The present invention relates to an apparatus and an automatic analysis method.
[0002]
[Prior art]
As is well known, immunoassays based on chemiluminescence have the advantages of very high sensitivity and high measurement reliability, but depending on the measurement item, dilution factor, number of washings, incubation time or number of reaction steps, etc. In many cases, the flow processing from sample dispensing to measurement was also various.
[0003]
For this reason, conventionally, dedicated flow processing devices having the same dilution rate, number of washings, incubation (incubation) time, and reaction steps have been installed for each measurement item with different flow processing.
[0004]
[Problems to be solved by the invention]
For this reason, conventionally, when processing is performed for each item, a dedicated flow processing dedicated device or a plurality of pipette devices must be prepared to perform measurement processing. There is a problem that equipment for each processing has to be installed and the equipment cost is increased, or there is a problem that processing cannot be performed unless various restrictions are added for each measurement process.
[0005]
The present invention was devised in view of the present situation. The purpose of the present invention is to first prepare separate containers for specimen dispensing and dilution, reagent dispensing, cleaning liquid dispensing, and the like. In this case, one processing item is not formed in a single step, but the containers used in the item are collectively formed at positions close to each other to facilitate the dispensing operation. In addition, by grouping items that perform the same processing such as reaction steps, and increasing the freedom of movement of the pipette device, it is automatically performed by a single processing device without installing multiple dedicated processing devices. It is an object of the present invention to provide an automatic analysis apparatus and an automatic analysis method that can perform processing.
[0006]
[Means for Solving the Problems]
In order to achieve the above object, as shown in FIG. 1, an automatic analyzer according to the present invention includes a pipette device 11 that performs a dispensing operation at a dispensing position according to a command, and a cartridge container loaded according to the command to a predetermined position. A cartridge container transfer means 12 for transferring, a measuring means 13 for measuring a sample in the cartridge container by a command, and a control device for transferring the cartridge container to a dispensing position and a measuring position to command dispensing and measurement. 14, and 14.
[0007]
In the present invention, the cartridge container is loaded into the cartridge container transfer means at a predesignated position, and the cartridge information at the designated position is inputted to the control device and can be collated. Is desirable.
[0008]
Of course, the cartridge container is provided with information about the cartridge container, and the information is read by a reading device arranged along the cartridge container transfer means, and the control device is based on the reading information. The cartridge container can be transferred to the dispensing position and the measurement position.
[0009]
In this invention, it is desirable that the pipette device has a nozzle that can move within a predetermined region and can suck and discharge liquid in the region according to a command from the control device. The nozzle is preferably provided with a magnet attachment / detachment control unit to the dispensing tip.
[0010]
In the present invention, the cartridge container transfer means transfers a cartridge container loaded in the vicinity of the thermostat bath by command, or rotates or serially transfers the radially loaded cartridge containers by command. Can be configured.
[0011]
Further, in the present invention, the measurement means is performed at the measurement position by means according to a measurement method.
[0012]
Further, the control device is configured to instruct sample dispensing, reaction, incubation, stirring, washing, and measurement to the pipette device, the cartridge container transfer device, and the measuring means when requested.
[0013]
Further, as shown in FIG. 2, the control device is loaded in an item designation means 16 for designating an item that can perform a series of processes using the same cartridge container, and the cartridge container transfer means 12. For the cartridge container, with respect to the item specified by the item specifying means 16, the number of cleaning steps included in each item, the specified number of items, the processing time of each item, the processing time of each step included in each item, or And a processing pattern setting means 20 for setting a processing pattern for each process based on the cartridge container position.
[0014]
Of course, the control device includes a loading instruction means 19 for urging the cartridge container transfer means 12 to load the corresponding cartridge container among the cartridge containers with information on the cartridge container including the identification information of the corresponding item, and The cartridge container loaded in the cartridge container transfer means 12 may be configured by attaching a cartridge container information reading means 18 for reading information relating to the cartridge container.
[0015]
As a preferred automatic analyzer that embodies the present invention, it is possible to move within a predetermined area according to a command, and to suck and discharge the specimen within the area. A magnet desorption control unit is provided, and dispensing is performed. A pipette device that attaches a tip and performs a dispensing operation at a dispensing position, a cartridge container transfer means that rotates and transports a radially loaded cartridge container to a predetermined position in the vicinity of the constant temperature bath by a command, and a command Optical measurement means for measuring the number of photons at a measurement position, and the control device designates an item designation means for designating an item capable of performing a series of processing using the same cartridge container, and the item designation For the items specified by the means, the number of cleaning steps included in each item, the number of specified items, the processing time of each item, or the processing time of each step included in each item A processing pattern setting means on the basis of the cartridge container position setting processing pattern for each process, it is desirable to construct a.
[0016]
Further, as another suitable automatic analyzer, it can be moved within a predetermined area by a command, a sample can be sucked and discharged within the area, a magnet desorption control unit is provided, a dispensing tip is attached, A pipette device that performs the dispensing operation at the dispensing position, a cartridge container transfer means for rotating and transporting a radially loaded cartridge container to a predetermined position in the vicinity of the thermostatic chamber by a command, and a photon count at the measurement position by the command. An optical measuring means for performing measurement, and the control device is applicable based on item specifying means for specifying an item capable of performing a series of processing using the same cartridge container, and identification information of the corresponding item. Loading instruction means for urging loading of the cartridge container into the cartridge container transfer means, and a cartridge loaded in the cartridge container transfer means For the container, the cartridge container information reading means for reading information relating to the cartridge container, and for the item specified by the item specifying means, the number of cleaning steps included in each item, the number of specified items, and the processing of each item It is also possible to have a processing pattern setting means for setting a processing pattern of each process based on the processing time of each process or the cartridge container position included in each item.
[0017]
Next, as an automatic analysis method for achieving the above object, according to the present invention, as shown in FIG. 3, an automatic analysis for performing a dispensing operation on a cartridge container transferred to a predetermined position by a pipette device. In the method, when an item that can perform a series of processes using the same cartridge container is specified (S1), the cartridge container is loaded into a predetermined loading position (S2) and loaded into a predetermined position of the cartridge transfer means. Check the number of cleaning processes included in each item for the specified item of the specified cartridge container, the specified number of items, the processing time of each item, the processing time of each process included in each item, or the cartridge container position After that (S3), the processing pattern of each process is set (S4), and each cartridge container is instructed to be transferred to the dispensing position and the measurement position (S5). It is characterized in.
[0018]
Further, as another automatic analysis method for achieving the above object, in the present invention, in the automatic analysis method for performing a dispensing operation on a cartridge container having a pipette device transferred to a predetermined position, the same cartridge is used. When an item that can perform a series of processing using a container is specified, the corresponding cartridge container is loaded into the transfer device from among the cartridge containers with information on the cartridge container including information for identifying the corresponding item. Instructs and reads the information about the loaded cartridge container, and for the specified item, the number of cleaning steps included in each item, the specified number of each item, the processing time of each item, or each step included in each item Set the processing pattern for each process based on the processing time or cartridge container position of It is characterized in that the command to transfer to a dispensing position and a measuring position.
[0019]
In the present invention, as shown in FIG. 4, the processing pattern is set by classifying items based on the number of cleaning steps included in each designated item (S14). In order to examine the number of each item, it is classified for each item (S15), further classified items are classified according to the processing time of the items (S16), and a processing pattern is set based on these classifications. Accordingly, the cartridge container is transferred to the dispensing position and the measurement position, and dispensing and measurement are commanded (S17).
[0020]
In the present invention, the “information regarding the cartridge container” includes, for example, information for identifying the cartridge container, information for identifying the corresponding item, and information for identifying the sample. Or displayed by other marks. Of course, the information about the cartridge container is not displayed on the cartridge container, but is input to the control device in advance in association with the positional information of the container transfer means such as a turntable on which the cartridge container is loaded. It can also be left.
[0021]
Further, in the present invention, “each step based on the number of cleaning steps included in each item, the number of specified items, the processing time of each item, the processing time of each step included in each item, or the cartridge container position” `` Set the processing pattern for '' even if the measurement items are different, processing the same reaction step together can save the program reading time and waste pipetting movement It is because it can be omitted.
[0022]
Further, by knowing the number of items of each item, the cartridge container transfer means performs processing in units of the number that can be transferred efficiently and with few operations within the time required for the process. Doing so will improve efficiency.
[0023]
In addition, since the items with the same processing time are the processing with the same contents, the time for reading a new program and the unnecessary movement of the pipette device are also prevented to increase the processing efficiency.
[0024]
Furthermore, in the present invention, normally, one item corresponds to one cartridge container and one sample.
[0025]
In the following description, the term “hole” refers to those having a bottom, and the term “hole” refers to those having no bottom.
[0026]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in detail based on embodiments shown in the accompanying drawings.
[0027]
FIG. 5 shows a top view of the automatic analyzer according to the first embodiment of the present invention. The automatic analyzer according to this embodiment transfers the loaded cartridge container 32 to a predetermined position in accordance with a command. A cartridge loading circular rotary stage (hereinafter referred to as “rotary stage”) 30 corresponding to the cartridge container transfer means 12 and an unused dispensing tip 36 which is mounted on a nozzle of a pipette device and performs a dispensing operation and the like are set up. And a sample rack 39 on which a sample container 38 in which a sample to be analyzed is stored is placed. . In FIG. 5, reference numeral 40 represents an area in which a nozzle unit 47c, which will be described later, can move.
[0028]
Furthermore, the rotary stage 30 rotates and transports a plurality of cartridge containers 32 loaded in a radial manner according to a command. Of course, you may comprise so that the cartridge container 32 may be transferred along the transfer path formed in series.
[0029]
The rotary stage 30 has a columnar fixed frame 31 that covers the upper surface and side surfaces of the rotary stage 30, and has a radius at the dispensing tip rack 37 and the sample rack 39 side of the upper surface of the fixed frame 31. A cutout portion 33 is provided along the direction so as to expose the upper surface of the cartridge container 32. The cartridge container 32 is exposed at the reading position by exposing the vicinity of the end portion on the center side of the cartridge container 32. In order to perform optical measurement to expose a range including the reading hole 34 for reading a bar code in the case of this embodiment, a bar code, and the optical measurement container of the cartridge container 32 as a measurement position. Measurement holes 35 are formed.
[0030]
In addition, a plurality of, for example, 50 lateral holes 45 for holding the loaded cartridge container 32 are provided radially along the radial direction inside the rotary stage 30, and the cartridge container 32 is loaded. A loading port 41 is provided at a fixed position on the outer peripheral portion of the fixed frame 31, and the loading port 41 and the opening of the horizontal hole 45 are controlled to coincide with each other when the rotary stage 30 is stopped. Each time one cartridge container 32 is loaded, the rotary stage 30 is arranged so that the opening of the adjacent open side hole 45 and the loading port 41 are aligned so that the next cartridge container 32 can be loaded. It is controlled to rotate.
[0031]
FIG. 6 is a cross-sectional view taken along line AA ′ of FIG. 5. As shown in FIG. 6, the rotary stage 30 has a rotating shaft 43 for holding and rotating the cartridge container 32. The horizontal hole 45 is formed together with the upper surface of the fixed frame 31 and a side plate (not shown), and a fixing plate 44 that functions as a high-temperature tank for applying heat to the cartridge container 32 according to a command, and holds the cartridge container. And a holding portion 52 that rotates.
[0032]
Further, the measurement hole 35 of the fixed frame 31 is provided with a PMT 42 as the measurement means for counting the number of photon photons, and a barcode as the information is placed above the reading hole 34 as the reading position. An optical mark reader OMR51 for reading is provided.
[0033]
FIG. 6 further shows a pipette device 47 provided above the rotary stage 30, the dispensing tip rack 37 and the sample rack 39.
[0034]
The pipette device 47 is a single-cylinder system and performs a dispensing operation by attaching a dispensing tip 36. A magnet attachment / detachment control unit is integrally provided on the dispensing tip 36, and B / F (antigen-antibody binding and release) is performed. A nozzle unit 47a capable of performing agitation and magnetic particle cleaning by separation and pumping (repetitive suction and discharge) of the mold, the nozzle unit 47a, the sample rack 39, the dispensing tip rack 37, and An XYZ moving stage 46 that can move with respect to a designated hole of the cartridge container in the notch 33 that is a dispensing position, a syringe 48 for performing dispensing work and cleaning of the nozzle unit 47a, and a cleaning container for containing cleaning liquid 50 and a switching valve 49 for switching the three-way pipes for sucking and discharging air or cleaning liquid. Reference numeral 48a denotes a motor for driving the syringe 48.
[0035]
Further, the PMT 42 moves the PMT 42 up and down with respect to the measurement hole provided at the end of the cartridge container 32, and counts the photons that are sealed and shielded. Of course, depending on the measurement item, a transmission measurement method, a spectroscopic measurement method, a turbidimetric method, or the like may be applied. Accordingly, the measurement hole 32k of the cartridge container 32 is formed to be transparent and optical measurement is performed. The device is formed corresponding to the measurement item.
[0036]
Next, a basic control system of the automatic analyzer 30 according to this embodiment will be described with reference to FIG.
[0037]
The control system of the automatic analyzer according to the present embodiment controls liquid dispensing, reaction, incubation (incubation: incubation particularly for high-temperature reaction), stirring, washing, and measurement.
[0038]
As shown in FIG. 7, the automatic analyzer 30 according to this embodiment includes a CPU and a memory 60 that perform various controls related to the automatic analyzer 30, an instruction for loading the cartridge container 32, display of analysis results, and the like. A bar code attached to the end closer to the center of the rotary stage 30 of the cartridge container 32 loaded in the rotary stage 30 corresponds to the display unit 65 for displaying the above and the cartridge container information reading means. And a barcode reading control unit 66 that reads and decodes the reading unit 51.
[0039]
Furthermore, a worksheet reading control for decoding a worksheet read by an optical mark reading unit (OMR), which corresponds to an item specifying means for specifying an item capable of performing a series of processing using the same cartridge container. Control unit 67, input / output unit 68 having a keyboard and mouse for inputting data, a printer device for recording and outputting data, a pipette device control unit 61 for controlling the pipetting device, and control of the rotary stage 30 A rotation stage control unit 62 that performs the control, a constant temperature control unit 63 that performs constant temperature control of a heater that is a constant temperature bath provided on the fixed plate 44 of the rotation stage 30, and a PMT control unit 64 that controls the PMT. In FIG. 7, reference numeral 72 denotes an XYZ stage controller that controls movement of the nozzle unit 47a in the XYZ directions, and reference numeral 73 denotes a pipette controller that controls the operation of the pipette.
[0040]
In addition, the CPU and the memory 60 store the number of cleaning steps included in each item, the number of specified items, and the processing of each item for items specified by the program via the worksheet reading control unit 67. Based on the time or the processing time or cartridge container position of each process included in each item, processing pattern setting means 69 for setting the processing pattern of each process and information on the cartridge container 32 including the identification information of the corresponding item are attached. Among the cartridge containers 32, the loading instruction means 70 for urging the corresponding cartridge container to be loaded on the rotary stage 30 and the analysis means 71 for analyzing the result obtained through the PMT control unit 64 are configured. Yes.
[0041]
Further, the memory stores in advance a program indicating the contents of each item and a processing procedure for processing each item. Of course, the CPU and memory 60 also store various control command signals related to the automatic analyzer.
[0042]
8 and 9 show a cartridge container 32 according to this embodiment.
[0043]
As shown in FIG. 8, the cartridge container 32 according to this embodiment includes a base portion 56 formed of a transparent body such as glass or plastic, and a knob 54 formed so as to protrude from the side surface of one end. The base portion 56 is provided with, for example, 11 holes (holes), and a bar code 56b to which information on the cartridge container 32 is input is attached to the upper surface of the knob 54.
[0044]
Of the eleven holes, the hole on the side closer to the knob side is a tip sack 55 for holding the dispensing tip 36, and the hole at one end is a measurement hole 32J for optical measurement. is there. The arrangement and number of these holes can be appropriately selected and formed according to the reaction step. Of course, each of the holes can be formed by inserting a separately molded container into a hole (not shown) formed in the base 56.
[0045]
The tip sack 55 is formed with a deep hole according to the length of the dispensing tip 36 so that the dispensing tip 36 can be held, and the liquid attached to the dispensing tip 36 is formed at the bottom of the tip sack 55. You may make it provide the water absorption pad 55a for absorbing a part. This chip sack 55 can also be molded separately.
[0046]
The measurement hole 32J is formed with a light shielding portion 53 that shields light from the outside by coating a light shielding film on the wall and bottom of the hole, or by stretching or integrally forming a light shielding film or a light shielding plate. . This is because light other than the light generated by the reaction is blocked during the optical measurement.
[0047]
Furthermore, from the body base side where eleven holes are provided in the area where the barcode 56b is attached or from the area where the barcode is attached, light passes through the upper surface of the transparent body and is used for measurement. For example, a light shielding groove is provided in the vicinity of the upper surface so as to cross the base portion 56 so as not to reach the hole 32J.
[0048]
The other holes 32A to 32I are formed so as to be transparent so that the inside can be seen.
[0049]
Further, a holding edge portion 56a is provided on the upper surface of the base portion 56, and the edge portion 56a is provided on the rotary stage 30 in a radial manner with a certain angle between the holding portion 52 and the adjacent side plate (not shown). ) Is fitted into the corresponding groove formed and held. FIG. 9 shows the cartridge container 32 in a perspective view.
[0050]
Next, the operation of the present embodiment will be described. The operator changes items representing processing to be inspected on the worksheet into marks of a format that can be read by the optical mark reading device corresponding to the item specifying means. Write on the sheet. In addition, the registration number of the patient is written on the worksheet by marks.
[0051]
Then, the optical mark reading device reads the worksheet. The worksheet is performed by marking the corresponding item among the items representing the process to be used for inspection, or by marking the number of the corresponding items on the mark. The read worksheet is decoded via the worksheet reading control unit 67.
[0052]
The loading instruction means 70 constituted by the CPU and the memory 60 instructs to load the rotary container 30 with the cartridge container 32 corresponding to the designated item. The instruction is performed by displaying the total number of items of the cartridge containers 32 to be loaded on the screen of the display unit 65.
[0053]
Based on the screen, the operator loads the designated number of cartridge containers 32 of the corresponding item one by one from the insertion port 41 of the rotary stage 30.
[0054]
In this way, the cartridge containers corresponding to the respective items are randomly loaded on the rotary stage 30.
[0055]
When one cartridge container 32 is loaded, the rotary stage 30 is rotated so that the vacant horizontal hole 45 located closest to the loaded position is aligned with the insertion port 41, thereby creating a new cartridge. The container 32 is set in an insertable state.
[0056]
In this way, when loading of the necessary cartridge container 32 into the rotary stage 30 is completed, the rotary stage 30 rotates once in accordance with instructions from the CPU and the memory 60, and the optical mark reading device 51 The bar code 56b attached to the end close to the center of the cartridge container 32 is read.
[0057]
Thereby, the CPU and the processing pattern setting means 69 of the memory 60 recognize the quantity and position of each item based on the read barcode attached to each cartridge container 32.
[0058]
Based on the recognition result, a processing pattern representing the order in which each item is to be processed is set as follows.
[0059]
The processing pattern is set so that many processes are performed in as short a time as possible, that is, the processing pattern is set so as to increase the processing efficiency. In addition, if possible, the operation of the rotary stage 30 should be reduced as much as possible.
[0060]
For this purpose, when the pipette device is attached to the dispensing position until the processing for one item, that is, one cartridge container is completed, and the processing is performed, when all of the items are concentrated. The processing time is the processing time of each item × the number of items, and enormous processing time is required.
[0061]
However, most of the processing time is the time for incubation (constant temperature reaction), and the pipette device 47 is vacant during that time. Therefore, the processing time can be shortened by using the time to perform other processing.
[0062]
For this purpose, if the same process is repeated, the same program can be used, it is not necessary to repeat the reading of the program, and the operation of the pipetting device can be kept to a minimum.
[0063]
Therefore, it is possible to improve efficiency by classifying the items and processing the same items or the similar items together.
[0064]
Then, if a plurality of cartridge containers are processed for the same process of a plurality of the same items, and the process proceeds to the next process after the process is completed, the pipette device is incubating each cartridge container. In the meantime, the same number of cartridge containers that can be processed within the incubation time can be processed in the same process, which is efficient.
[0065]
Here, a time I such as incubation in step i of an item j^j _iThe number n that can be processed within^jIs the time P required by the pipetting device in the process^j _i, Time R until the same cartridge container is transferred and returned to the dispensing position againⁿDepends on (number n^jAlso depends on).
That is,
P^j _i× n^j+ Rⁿ≦ I^j _i  …formula
Holds.
N determined from the formula^jWhen processing for the number of items, the n^jThe time required to process each item is approximately ΣⁱI^j _i(I^j _iIs taken for i).
[0066]
At that time, if the cartridge container is transferred by rotating the rotary stage 30 in the forward direction, RⁿCan be shortened. When processing each item independently, n^j× ΣⁱI^j _iTime is required.
[0067]
Considering the above points, the processing pattern setting means 69 according to the present embodiment sets the processing pattern as follows.
[0068]
First, for the set item, data representing the contents of each item stored in advance in the memory is read.
[0069]
Next, the number of cleaning steps included in each item step is detected.
[0070]
Classify each item by the number of cleaning steps. The difference in the number of cleaning steps basically defines the processing procedure for each item, such as the difference in the number of dispensing operations performed in each item and the difference in processing time. Items having the same number of cleaning steps are similar in processing to each other.
[0071]
Each item divided by the number of cleaning steps is classified for each item, and the number of each item is examined.
[0072]
Next, the incubation process (time) of each item is examined, and the treatment pattern is set by classifying by the incubation time.
[0073]
For example, it is assumed that the cleaning process includes A, D, and E as items of one step, the cleaning process includes B and C as items of two steps, and the cleaning process includes F and G as items of three steps. .
[0074]
Here, the difference in items in each step is caused by a difference in the type of reagent or labeling agent used.
[0075]
Further, for each step, the number of items is checked by classifying the items. For example, in one step, 15 samples are set for item A, 11 samples are set for item D, and 14 samples are set for item E.
[0076]
In addition, when classified by the incubation time of each item, that is, the time required for item processing, for example, if item A has a processing time of 20 minutes, item D has a processing time of 32 minutes, and item E has a processing time of 20 minutes, Since item E is 20 minutes, it is classified into the same group, and item D is classified into another group because it is 32 minutes.
[0077]
This difference in processing time is caused by the fact that it is necessary to carry out processing with different incubations even when the same number of washing steps are used and the same labeling agent or the like is used.
[0078]
At that time, when the number of cleaning steps is 1, n determined from the above formula^jFrom the above, when the rotation stage 30 makes one rotation, 5 cartridge containers / 30 minutes are possible, and when the number of cleaning processes is 2, 4 cartridge containers / 30 minutes are possible, and the number of cleaning processes is 3. In this case, a case where it is efficient to use three cartridge containers / 30 minutes will be described.
[0079]
In this case, when the number of cleaning steps is 1, the processing pattern setting unit 69 first performs five processings for each of the items A three times, and then performs two processings for each of E five times. finish. Next, the item D is set to be processed twice every five.
[0080]
Then, for the item E, 4 samples remain, and for the item D, 1 sample remains.
[0081]
In the same manner, the processing is performed when the number of cleaning steps is 2, and the processing when the number of cleaning steps is 3 is performed.
[0082]
After the above batch processing is completed, the remaining items are processed in each cleaning step. For example, in the case of the cleaning step 1, the processing is performed for four specimens of item E and one specimen of item D. Set.
[0083]
In this way, the processing pattern setting unit 69 sets the most efficient processing pattern for the designated item, and according to the processing pattern, the rotary stage control unit 62, the pipette device control unit 61, and the PMT control unit. 64 and the constant temperature control unit 63 are instructed to control.
[0084]
Next, a process will be described in the case where an immunochemical test is specifically performed by the chemiluminescence method according to the instruction.
[0085]
As shown in FIGS. 5 and 6, the dispensing tip 36 attached to the nozzle unit 47 a of the pipette device 47 from the dispensing tip rack 37 is supplied from the sample container 38 in the sample rack 39. In addition, a reaction insoluble magnetic substance liquid, a cleaning liquid, an enzyme labeling liquid, a substrate liquid, a reaction stopping liquid, and the like are discharged or aspirated into the cartridge container 32.
[0086]
In addition, as shown in FIG. 8, the dispensing tip 36 includes, for example, the most detailed portion 36 a inserted into the holes 32 </ b> A to 32 </ b> J of the cartridge container, the medium diameter portion 36 b having a larger diameter than the most detailed portion 36 a, The large-diameter portion 36c that is larger in diameter than the medium-diameter portion 36b is formed in a three-stage shape. A magnet that adsorbs the reaction-insoluble magnetic material is detachably disposed on the outer peripheral surface of the medium-diameter portion 36b, and the dispensing tip 36 is attached to the nozzle unit 47a. When the liquid is sucked into the dispensing tip 36, the magnet in the liquid is surely collected by the magnet.
[0087]
The cartridge container 32 is not limited to the case where the holes 32A to 32J are provided in series, but may be configured in a line shape such as a loop shape or a zigzag shape.
[0088]
The sample is roughly dispensed in advance in the hole 32C, the required amount of reaction insoluble magnetic substance liquid is stored in the hole 32D in advance, and the required amount of cleaning liquid is previously stored in the hole 32E and the hole 32F. A required amount of labeling liquid is previously stored in the hole 32G, a required amount of cleaning liquid is previously stored in the hole 32H and the hole 32I, and a substrate solution is further stored in the measurement hole 32J. The luminescent state is measured by dispensing.
[0089]
At this time, it is assumed that, for example, five cartridge containers 32 corresponding to the item A for performing the same processing are already loaded on the rotary stage 30.
[0090]
First, a sample that has been roughly dispensed into the hole 32C of one of the cartridge containers 32 that has stopped at the dispensing position 33 is aspirated by the dispensing tip 36 to be quantified. The dispensing tip 36 on which the roughly dispensed sample has been aspirated is transferred by the XYZ moving stage 46, and after discharging the entire amount of the sample aspirated into the reaction insoluble magnetic body fluid in the hole 32D, the sample and the reaction insoluble magnetic material are discharged. The liquid mixture with the body fluid is repeatedly sucked and discharged (pumping) by the dispensing tip 36 to generate a uniform stirring and mixing state of the magnetic substance.
[0091]
Then, the dispensing tip 36 was transferred to the position of the tip sack 55, removed from the nozzle unit 47a, held in the tip sack 55, and then loaded by rotating the rotary stage 30 in the forward direction. The other four cartridge containers 32 are rotated and transferred to the notch 33 which is the dispensing position, and the same process is performed.
[0092]
After a certain period of time, the first cartridge container 32 is rotated and returned to the notch 33 which is the dispensing position.
[0093]
Here, the fixed time is a time required for the initial incubation of the cartridge container 32, and a process requiring a reaction, for example, after mixing the specimen and the reaction-insoluble magnetic substance liquid or after dispensing the labeling liquid, After dispensing of the substrate solution and the reaction stop solution, it is carried out after a step requiring a certain reaction time.
[0094]
Then, the nozzle unit 47a is transferred to the position of the tip sac 55 of the first cartridge container 32, and the dispensing tip 36 held in the tip sack is attached, and held in the incubated hole 32D. The mixed liquid that has been used is sucked by the dispensing tip 36.
[0095]
At this time, when the magnetic substance floating in the mixed solution sucked by the dispensing tip 36 passes through the middle diameter portion 36 b of the dispensing tip 36, the magnet M disposed outside the dispensing tip 36. Is collected on the inner wall surface of the medium diameter portion 36b. Further, the suction height of the mixed liquid is sucked by the dispensing tip 36 so that the lower surface of the mixed liquid is at the same level as the lower end of the magnet M when all the mixed liquid is sucked. It is considered to be collected by.
[0096]
After the magnetic material is collected in this manner, the mixed liquid excluding the magnetic material is discharged into the hole 32D and discharged, and only the magnetic material remains in the dispensing tip 36.
[0097]
Next, the dispensing tip 36 is sent to the next hole 32E while collecting the magnetic material, and sucks the cleaning liquid in the liquid storage portion 32E. At this time, the magnet M moves in a direction away from the dispensing tip 36 to release the attracted state of the magnetic material. Therefore, by pumping the cleaning liquid, the entire magnetic material can be efficiently cleaned. it can.
[0098]
After the completion of the pumping, the dispensing tip 36 sucks a certain amount of the cleaning liquid in the hole 36E. At this time, the magnet M again approaches the dispensing tip 36 and collects all the floating magnetic material in the suctioned cleaning liquid, and the cleaning liquid excluding this magnetic body is discharged into the hole 32E and discharged. Only the magnetic material remains in the dispensing tip.
[0099]
Next, the dispensing tip 36 is sent to the next hole 32F while collecting the magnetic material, sucks the cleaning liquid in the hole 32F, and performs the same procedure as the procedure performed in the hole 32E. Cleaning and collection operations are performed.
[0100]
Next, the dispensing tip 36 is sent to the next hole 32G while collecting the washed magnetic material, and sucks the labeling liquid in the hole 32G. At this time, the magnet M moves in a direction away from the dispensing tip 36 to release the adsorbed state of the magnetic substance. Therefore, by pumping the labeling liquid, the reaction between the whole magnetic substance and the labeling liquid is made uniform. It can be made.
[0101]
Then, after the pumping is finished and the incubation is finished, the dispensing tip 36 sucks a certain amount of the labeling solution in the hole 32G. At this time, the magnet M again approaches the dispensing tip 36, collects all the floating magnetic material in the attracted labeling liquid, and the labeling liquid excluding this magnetic body is discharged into the hole 32G. It is discharged and only the magnetic substance remains in the dispensing tip 36.
[0102]
Thereafter, the dispensing tip 36 is sent to the next hole 32H while collecting the magnetic material, and the cleaning liquid in the hole 32H is sucked, and the magnetic material is cleaned in the same procedure as the holes 32E and 32F. After collection, the cleaning liquid in the next hole 32I is sucked in the same procedure as the cleaning liquid suction procedure in the hole 32H, and the magnetic material is cleaned and collected.
[0103]
Thereafter, the dispensing tip 36 is transferred to the hole 32J, and when the reaction time is required for a certain time, the substrate liquid previously stored in the hole 32J is sucked. At this time, the magnet M moves in a direction away from the chip 36 in the sentence to release the adsorbed state of the magnetic substance. Therefore, by pumping the substrate liquid, the reaction between the whole magnetic substance and the substrate liquid is made uniform. be able to.
[0104]
When the pumping is completed, the dispensing tip 36 is again transferred to and held at the tip sack 55 position.
[0105]
Then, the rotary stage 30 is rotated, and the other four cartridge containers 32 are successively rotated and transferred to the dispensing position 33 to perform the same process.
[0106]
After a certain period of time, the measurement hole 32J of the first cartridge container 32 is rotated and transferred so as to reach the measurement hole 35 at the measurement position shown in FIG. Here, the fixed time is the time required for incubation for the first cartridge container 32.
[0107]
At this measurement position, the light emission amount is measured by an optical measurement device having a configuration corresponding to a predetermined measurement method such as PMT42.
[0108]
Thereafter, the dispensing tip 36 and the first cartridge container 32 are discarded. When the measurement for the first cartridge container 32 is completed, the other four cartridge containers 32 are also rotated and transferred to reach the measurement holes 35 one after another. The container 32 is discarded.
[0109]
In this embodiment, after the pumping in the hole 32D and before the measurement in the hole 32J, the rotary transfer is performed by the rotary stage. However, the present invention is not limited to this case. Each process may be rotated or transferred in series.
[0110]
10 to 13 show a second embodiment of the cartridge container 132 used in the automatic analyzer of the present invention. The cartridge container 132 according to this embodiment is a container body integrally formed of glass, plastic, or the like. 156 and a knob 154 formed at one end of the container main body 156. The container main body 156 has nine pieces which are two pieces smaller than the hole of the cartridge container 32 of the first embodiment. Holes 132A to 132I and a container holding hole 132J for detachably holding the measurement container 133 are formed.
[0111]
That is, unlike the cartridge container 32 used in the first embodiment, the cartridge container 132 according to this embodiment is not formed with the tip sack 55 that holds the dispensing tip 36, and the cartridge container 132 includes The cartridge container 32 is different from the cartridge container 32 according to the first embodiment in that no display means such as a barcode label for displaying information of the cartridge container 132 is provided.
[0112]
Thus, by not forming the tip sack for holding the dispensing tip 36 in the cartridge container 132, the configuration of the cartridge container 132 is greatly simplified as compared with the cartridge container 32 according to the first embodiment. As a result, the manufacturing cost can be greatly reduced, and the length of the cartridge container 132 can be significantly shortened as compared with the cartridge container 32 of the first embodiment. When loaded on the rotary stage 30, the diameter of the rotary stage 30 can be reduced, and this type of automatic analyzer can be further miniaturized.
[0113]
Further, the cartridge container 132 is not provided with a display means such as a bar code label for displaying the information of the cartridge container 132, and the information of the cartridge container 132 is input to the control device 14 in advance, By inputting the loading position information to the rotary stage 30 to the control device 14 and clarifying the correspondence relationship, the barcode reading device such as the automatic analyzer according to the first embodiment can be eliminated. As a result, the processing step for controlling the rotation of the rotary stage 30 for reading cartridge information can be omitted, the processing pattern can be greatly simplified, the processing speed can be greatly increased, and the apparatus can be used. It can be simplified to reduce costs.
[0114]
By the way, the inside of the holes 132A to 132I is formed of transparent plastic or glass so that the contents can be seen through from the outside in this embodiment. The inner wall and the bottom of the measurement container 133 formed in step 1 are coated with a light-shielding film so as to reliably measure weak chemiluminescence. That is, the cartridge container 132 according to this embodiment is formed of two parts, a container body 156 that is a transparent body and a measurement container 133.
[0115]
Of course, as another means for configuring the measurement container 133 so as to reliably measure weak chemiluminescence, the inner wall and the bottom of the measurement container 133 are subjected to a process such as attaching a light shielding film or a light shielding plate, and assembled together. 3 parts, or the container body 156 itself may be made opaque with a material excellent in light shielding properties, or may be integrally formed by coloring with excellent light shielding properties such as black or white. .
[0116]
Further, when the measurement container 133 is used as a transparent body, the container holding hole 132J is formed in a bottomed shape, and an inner surface of the container holding hole 132J is coated with a light shielding film, or is integrally formed, or It is desirable to assemble them integrally by stretching a light shielding plate, or to apply a color having excellent light shielding properties such as black or white.
[0117]
Of course, as shown in FIG. 13, the measurement container 133 may be formed integrally with a hole row formed in the container main body 156 as a measurement container hole. In this case, the measurement container hole The light shielding layer is coated with a light-shielding film on the inner wall and the bottom of the part, or is integrally assembled by attaching a light-shielding plate, etc., or is colored with excellent light-shielding properties such as black or white. It is desirable to form 133A.
[0118]
By forming the measurement container 133 or the measurement container hole in this manner, for example, when the measurement container 133 is used for chemiluminescence measurement, light other than the light generated by the reaction can be blocked. it can. Of course, depending on the measurement method such as the transmission measurement method, the spectroscopic measurement method, or the turbidimetric method, it may not be necessary to shield the light, and in this case, it is used as it is transparent.
[0119]
In addition, the arrangement | positioning site | part of the said measurement container 133 or the measurement container hole part is not limited to the example of illustration, Of course, it can form in an appropriate position according to the reaction process number etc. of a measurement item. is there.
[0120]
The nine holes 132A to 132I have a substantially elliptical planar shape, and each bottom portion 134 has a substantially V-shaped cross section (intersection angle is 90 ° in the illustrated example). As shown in FIG. 10, a groove 135 having a substantially concave cross section is formed in the inner bottom part 134a of each bottom part 134 along the inclined surface of each inner bottom part 134a.
[0121]
The groove 135 is formed so that the width dimension thereof is smaller than the diameter dimension of the distal end portion of the dispensing tip 36, and the length of the groove 135 is larger than the diameter dimension of the distal end portion of the dispensing tip 36. Since the sample / reagent contained in each of the holes 132A to 132I flows through the groove 135 and sucks the entire amount even if the tip of the dispensing tip 36 abuts on the inner bottom 134a. Can guarantee the exact quantitativeness of this type of equipment, and eliminate the waste of samples and reagents.
[0122]
Even if the tip of the dispensing tip 36 is brought into contact with the inner bottom portion 134a of each of the holes 132A to 132I when the sample / reagent is discharged into the holes 132A to 132I due to the presence of the groove 135, FIG. As shown in FIG. 10, since the sample / reagent to be discharged flows out from the groove 135 into each of the holes 132A to 132I on the average in the left-right direction, the stirring flow by the discharge of the sample / reagent is in the holes 132A to 132I. Therefore, a uniform reaction state can be obtained.
[0123]
It should be noted that the number of the holes 132A to 132I is not limited to the illustrated example, and can be formed to an appropriate number corresponding to the number of reaction steps of the measurement item. The form is not limited to that shown in the figure, and for example, it may be formed by interspersing protrusions.
[0124]
As described above, the cartridge container 132 according to this embodiment, when sucking and discharging the sample / reagent in the cartridge container 132, does not block the distal end portion of the dispensing tip 36, and performs quantitative suction / quantitative discharge. The pipette device can be used for reliable dispensing and agitation work. In particular, since the entire amount in the cartridge container 132 can be aspirated, an extra sample / reagent is required. However, since the entire amount is handled as a fixed amount, it is possible to realize a highly accurate inspection for the entire amount, and since the configuration is simple, an excellent effect that it can be provided at a low price is obtained. .
[0125]
In the first and second embodiments, the reagent required for the reaction step is dispensed in advance into the predetermined holes of the cartridge containers 32 and 132 in accordance with the purpose by using one dispensing tip 36. However, the present invention is not limited to this. For example, a cartridge container is used. A plurality of reaction processing columns having the same structure as 32 and 132 are formed in a rack shape, and there are a plurality of pipettes and / or dispensing tips corresponding to the reaction processing columns. The processing speed can be further improved by processing the racks that have been processed at the same time and transported to the next processing position, for example, the measurement position, by the rotating stage. .
[0126]
In each of the above embodiments, the case where the cartridge container is configured to be washed twice after discharging the reaction-insoluble magnetic substance liquid and twice after discharging the labeling liquid has been described as an example. However, the present invention is not limited to this, and may be configured to be washed once or more as necessary.
[0127]
In the above embodiment, the case where both the dispensing tip 36 and the cartridge containers 32 and 132 can be transferred has been described as an example. However, only the raising and lowering of the dispensing tip 36 is performed, and the cartridge containers 32 and 132 are intermittently transferred. An operation process may be performed.
[0128]
Further, in each of the above-described embodiments, the case where the dispensing tip 36 and the cartridge containers 32 and 1312 are made disposable has been described as an example. However, the dispensing tip 36 and the cartridge containers 32 and 132 can be washed and reused. Also good.
[0129]
Further, in the above embodiment, the case where the magnet is disposed on one side of the middle diameter portion of the dispensing tip so as to be freely separated from each other has been described as an example, but the magnet may be disposed on both sides with the middle diameter portion interposed therebetween, Further, the collection efficiency of the magnetic material can be further improved by repeatedly raising and lowering the dispensing tip itself.
[0130]
【The invention's effect】
According to the invention described in claim 1, since the cartridge container can be transferred not only to the dispensing position but also to the measurement position, the dispensing operation and the measurement operation can be performed automatically without human involvement. Since the measurement can be performed reliably and easily, the burden on the operator can be reduced and reliable measurement can be performed.
[0131]
According to the invention described in claims 2 and 3, the cartridge container can be automatically transferred not only to the dispensing position and the measurement position, but also to the reading position. Since it can be performed automatically and reliably without human involvement, the burden on the operator can be further reduced.
[0132]
According to the fourth aspect of the present invention, not only the cartridge container but also the pipette device itself is provided to be movable within a predetermined range. Therefore, in addition to the effects of the inventions of claims 1 and 2, the scope of operation is expanded, and analysis of various samples and analysis of a large number of samples can be performed easily and reliably with human intervention. Can be done without.
[0133]
According to the invention described in claim 5, since the magnet attachment / detachment control unit to the dispensing tip is provided in the nozzle, the immunochemical test method using the magnetic substance ensures the collection of the magnetic substance in a short time. Therefore, simplification, simplification, generalization, cost reduction, and multi-channeling of an immunochemical test apparatus using a magnetic material can be realized.
[0134]
According to the invention described in claim 6, since the cartridge container transfer means is provided in the vicinity of the thermostatic bath, the cartridge container is transferred to give a uniform temperature distribution to the cartridge container, and the temperature of each cartridge container is Can be kept uniform, a stable measurement result can be obtained, and the reliability with respect to the measurement accuracy is greatly improved.
[0135]
According to the seventh aspect of the present invention, since the cartridge container transfer means is configured to rotate and transport the cartridge container that is radially loaded, the cartridge container can be removed without taking up space and with simple control. It can be reliably transferred. Further, by providing a thermostatic bath in the vicinity of the cartridge container transfer means, a uniform temperature can be given to the cartridge container by rotational transfer.
[0136]
According to the invention described in claim 8, since the reaction state can be automatically measured based on the measurement method corresponding to the analysis purpose, there is no need for an operator, and the burden on the operator is reduced. A reliable and reliable measurement can be performed.
[0137]
According to the ninth aspect of the present invention, since dispensing, reaction, incubation, stirring, washing and measurement can be commanded by the control device, it is not necessary for the operator to intervene and the burden on the operator can be reduced. , Reliable and reliable processing can be performed.
[0138]
According to the invention described in claim 10, since the processing pattern setting means and the item specifying means are provided, the processing pattern is set so that the automatic analyzer is most efficient. Each item can be processed quickly, economically and efficiently.
[0139]
According to the eleventh aspect of the invention, in addition to the effects of the tenth aspect of the invention, the cartridge container information reading means and the cartridge container loading instruction means are provided, so that there is no human intervention. The automatic analyzer can be operated most efficiently.
[0140]
Further, according to the inventions of claims 12 and 13, the effects obtained from the inventions of claims 1 to 11 can be enjoyed at the same time. A simplified general-purpose automatic analyzer can be obtained at low cost.
[0141]
According to the method of the present invention, the number of cleaning steps included in each item, the number of specified items, the processing time of each item, or the processing time of each step included in each item Alternatively, since the process pattern of each process is set based on the cartridge container position, the process can be performed quickly, economically and efficiently, and the application field thereof includes, for example, a magnetic body and a magnetic body. It can be applied to reactions that occur between liquids that do not contain, or substances that are present in liquids, and physical and chemical attachments to magnetic materials. Immunological substances such as antigens, antibodies, proteins, enzymes, DNA, vector DNA, RNA or plasmids, biological substances or molecular substances, or isotopes, enzymes, chemiluminescence necessary for qualitative / quantitative analysis, fluorescence It can be applied to immunological tests, chemical reaction tests, DNA extraction / recovery / isolation devices, etc. in addition to testing methods or clinical testing devices for labeling substances used for light, electrochemiluminescence, etc. .
[Brief description of the drawings]
1 is a principle block diagram according to the first aspect of the present invention;
FIG. 2 is a principle block diagram according to the invention of claim 9 or claim 10;
FIG. 3 is a principle flowchart according to the invention as set forth in claim 14;
FIG. 4 is a principle flowchart according to the invention as set forth in claim 15;
FIG. 5 is a top view of an automatic analyzer according to an embodiment of the present invention.
FIG. 6 is a cross-sectional view taken along line A-A ′ of the automatic analyzer.
FIG. 7 is a block diagram showing a control system of the automatic analyzer.
8A is a top view of a cartridge container applied to the automatic analyzer, and FIG. 8B is a cross-sectional view thereof.
FIG. 9 is a perspective view showing the cartridge container.
FIG. 10 is a plan view showing another embodiment of the cartridge container used in the automatic analyzer according to the present invention.
FIG. 11 is a front view of the cartridge container.
FIG. 12 is a sectional view of the cartridge container.
FIG. 13 is a partial sectional view of the cartridge container.
[Explanation of symbols]
11,47 Pipette device
12 (30) Cartridge container transfer means (rotary stage)
13 (42) Measuring device (PMT)
14, 16 (60, 61, 62, 63, 64, 66, 67) Control device
17 Item designation means
18 Cartridge container information reading means
19, 70 Loading instruction means
20, 69 processing pattern setting means
32,132 cartridge container
36 dispensing tips

Claims (15)

There rows dispensing work in the dispensing position by a command, a pipetting device having a nozzle having a dispensing tip collecting possible magnet detachable control unit and the reaction insoluble magnetic material on the inner wall of the cartridge container loaded by a command Cartridge container transfer means for transferring the sample to a predetermined position, measurement means for measuring the sample in the cartridge container by command , liquid dispensing , reaction, incubation, B / F separation, agitation, washing , if required And a control device for instructing the pipette device, the cartridge container transfer means, and the measurement means to perform the measurement . 2. The cartridge container has a plurality of holes corresponding to the number of reaction steps of a measurement item, at least one hole accommodates a reaction insoluble magnetic substance liquid, and at least one hole is a measurement hole. Automatic analyzer described in 1.   The cartridge container is loaded into the cartridge container transfer means at a predesignated position, and cartridge information at the designated position can be input to the control device and collated. The automatic analyzer according to claim 1. Information about the cartridge container is given to the cartridge container, and the information is read by a reading device disposed along the cartridge container transfer means, and the control device automatic analyzer according to claim 1 or claim 2, characterized in that transferring the cartridge container. The pipette device, by a command of the control device, movable in a predetermined area, to one of the claims 1 to 4, characterized in that it has a nozzle capable of suction and discharge of liquid in the region The automatic analyzer described. 6. The automatic analyzer according to claim 1, wherein the cartridge container transfer means transfers the loaded cartridge container by command under the influence of a thermostatic bath.   7. The automatic analyzer according to claim 6, wherein the cartridge container transfer means rotates or serially transfers the radially loaded cartridge containers according to a command.   The automatic analyzer according to any one of claims 1 to 7, wherein the measurement means is performed at the measurement position by means according to a measurement method. The control device is designated by the item designating unit for an item designating unit for designating an item capable of performing a series of processes using the same cartridge container, and a cartridge container loaded in the cartridge container transporting unit. The processing pattern of each process based on the number of cleaning processes included in each item, the number of specified items, the processing time of each item, the processing time of each process included in each item, or the cartridge container position automatic analyzer according to any one of claims 1 to 8, characterized in that it has a, a processing pattern setting means for setting. The control device includes a loading instruction means for urging the cartridge container transfer means to load a corresponding cartridge container among cartridge containers with information on cartridge containers including identification information of corresponding items, and the cartridge container transfer means. The automatic analyzer according to claim 9 , further comprising a cartridge container information reading unit that reads information about the cartridge container. A magnet attachment / detachment control unit that can move within a predetermined area according to the command, can absorb and discharge the specimen within the area, and collect a reaction insoluble magnetic substance on the inner wall surface of the dispensing tip is provided. A pipette device that is mounted and performs a dispensing operation at a dispensing position, a cartridge container transfer means for transferring a loaded cartridge container to a predetermined position under the influence of a thermostatic chamber according to a command, and a photon at a measurement position according to the command optical measuring means to measure the number, item designation means for performing designation of items that can be carried out a series of processes using the same cartridge container, and, with respect to items specified by said item specifying means, each item the number of washing steps included in each item number is specified, the processing time for each item based on the processing time and the cartridge container position of each process included in each item, each step The processing pattern representing the physical order, which has a processing pattern setting means for setting, on the basis of the processing pattern, liquid dispensing, reaction, incubation, B / F separation, agitation, cleaning and the pipetting device an instruction of the measurement, And a control device for instructing the cartridge container transfer means and the optical measurement means to perform the automatic analysis apparatus. A magnet attachment / detachment control unit that can move within a predetermined area according to the command, can absorb and discharge the specimen within the area, and collect a reaction insoluble magnetic substance on the inner wall surface of the dispensing tip is provided. A pipette device that is mounted and performs a dispensing operation at a dispensing position, a cartridge container transfer means for transferring a loaded cartridge container to a predetermined position under the influence of a thermostatic chamber according to a command, and a photon at a measurement position according to the command item designation means for performing an optical measuring means to measure the number, the designation of items that can be carried out a series of processes using the same cartridge container, said cartridge container for the relevant cartridge container based on the identification information of the corresponding item loading instruction means for prompting the loading of the transport means, the loaded cartridge container to the cartridge container transfer means, the cartridge Cartridge container information reading means for reading information on the container, and the relative items specified by the item specifying means, the number of washing steps included in each item, the number of the specified item, the processing time of each item, each item A processing pattern setting means for setting a processing pattern representing the processing order of each process based on the processing time of each process and the cartridge container position, and based on the processing pattern , liquid dispensing, reaction, An automatic analysis comprising: a control device for instructing incubation, B / F separation, stirring, washing and measurement to the pipette device, cartridge container transfer means and optical measurement means. apparatus. A pipette device having a nozzle equipped with a magnet desorption control unit capable of collecting a reaction-insoluble magnetic substance on the inner wall surface of a dispensing tip, and performs dispensing work on the cartridge container transferred to a predetermined position by the cartridge container transfer means. In the automatic analysis method in which measurement is performed on the sample in the cartridge container by the measurement unit, if there is designation of an item that can perform a series of processing using the same cartridge container, the predetermined of the cartridge container transfer unit The number of cleaning steps included in each item for the specified item of the cartridge container loaded at the position, the specified number of items, the processing time of each item, the processing time of each step included in each item , and the cartridge container based on the position, it sets the processing pattern defining a processing order of each step, based on the processing pattern, liquid dispensing, reactive Incubation, B / F separation, agitation, so as to perform cleaning and measurement, the pipette device, instructs the cartridge container transfer means and the measuring means, an automatic analyzer wherein the. A pipette device having a nozzle equipped with a magnet desorption control unit capable of collecting a reaction-insoluble magnetic substance on the inner wall surface of a dispensing tip, and performs dispensing work on the cartridge container transferred to a predetermined position by the cartridge container transfer means. In the automatic analysis method in which measurement is performed on the sample in the cartridge container by the measuring means, if an item that can perform a series of processing using the same cartridge container is specified, the corresponding item is identified Instructs loading of the corresponding cartridge container among the cartridge containers with information on the cartridge container including information into the transfer device, reads the information about the loaded cartridge container, and is included in each item for the designated item the number of washing steps, each item number is specified, the processing time of each item, the processing of each process included in each item Based during and cartridge container position, it sets the processing pattern defining a processing order of each step, based on the processing pattern, with each cartridge container is transferred from the reading position, liquid dispensing, reaction, incubation, B / F: An automatic analysis method instructing the pipette device, the cartridge container transfer means and the measurement means to perform separation, stirring, washing and measurement . The setting of the processing pattern classifies items based on the number of cleaning steps included in each designated item, classifies each item for examining the number of designated items for each classified item, The classified items are classified according to the processing time of the items, and based on these classifications, a processing pattern is set, and according to the processing pattern , liquid dispensing , reaction, incubation, B / F separation, stirring, washing and measurement are performed. to perform the pipetting device, automatic analysis method according to any one of claims 13 or claim 14, characterized in that instructs the cartridge container transfer means and the measuring means.

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