JP3482558B2 - An anti-dioxin health food extracted from fermented soybeans and containing genistein as a main ingredient. - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a health food having an anti-dioxin effect, and relates to an anti-dioxin health food containing genistein as a main raw material capable of excreting environmental hormones and harmful substances which are free in the body. It is a thing.

[0002]

In the prior art, isoflavone aglycone contained in soybean is known to have three kinds of mixed aglycones such as genistein, daidzein and glycitein, which have physiological effects such as antioxidation, antiarteriosclerosis, anticancer and antiosteoporosis. There is.

There are about 70 kinds of substances suspected as environmental hormones. There are many 50,000 to 80,000 kinds of chemical substances around us, which are “environmental hormones”. Dioxins are medicines, pesticides, chemicals,
It is generated from environmental pollutants such as insecticides and garbage, and has a function similar to that of human hormones even though it is a minute amount, it disturbs endocrine and weakens the fertility of animals. If dioxin binds to cells in the body, it cannot be removed for a long period of time.
Over time, the concentration increases and cancer occurs. Due to the influence of environmental hormones, cancers related to sex hormones such as breast cancer and prostate cancer have been increasing in recent years. With humans living in fear, anti-dioxin methods are currently unsolved. In addition, there is no health food that has that effect.

[0004]

The problems to be solved by the invention are (1) separation of a single genistein from isoflavone aglycone, (2) dioxins not being bound to human hormones in the body, and accumulation of dioxins in the body. It is to reduce (3) to combine with dioxin that has entered the body to be discharged to the outside, and (4) to increase the immune system of the human body and prevent toxicity of dioxin.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION An anti-dioxin health food containing genistein extracted from fermented soybean as a main raw material. A healthy food that mixes genistein with plant fibers etc. to eliminate environmental hormones and harmful substances that are free in the body.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION An anti-dioxin health food as a single product is prepared by mixing genistein extracted from fermented soybean with other natural plants, and an anti-dioxin health food seasoning is prepared as a powdery mixture. Used as.

[0007]

EXAMPLE Extract genistein. Soybean is fermented for 12 months, of which about 90% is isoflavone aglycone, which is further extracted and purified by HPLC to obtain genistein (Fig. 2).

When genistein is used as the main raw material and dioxin does not bind to human hormones and is excreted, immunity is increased. (1) Prevent dioxin from stopping inside the body. Eating 100 ug / kg / day of genistein contained in the health food of the present invention (in recent years, according to statistics, Japanese soybean is about 30-50 g / day, genistein is equivalent to 1 / 1,000th of soybean). There is.
(2) Emit dioxin. Among these health foods, genistein, agarix, aloe vera, ryokcha,
Since chitin / chitosan and beta / carotene are rich in green plants and plant fibers, they can regulate metabolism in the living body and excrete free dioxin and harmful substances in the body.

Prevent the toxicity of dioxins. Since there are specific glycosides with an anti-poisonous effect in health foods,
Increase immune cells. At the same time, it has a function of suppressing the growth of cancer cells generated by dioxin. For example, this health food contains red ginseng, specific glycosides and enzymes such as agarix, chitin / chitosan, garlic extract, beta-carotene, etc. (1) It has an antitoxic effect, (2) NK cell increase (3) It has an antioxidant effect, and in particular, has an effect of increasing the mass of eggs and sperm. This health food is not just a cure for the disease, it is also aimed at preventing the disease.

The plant combined with genistein and the anti-dioxin excretion action were examined. It showed a synergistic effect with the effect of selecting aloe, ryokchicha, adlay extract, etc., among several tens of plants. These were the best ejectors.

[0011]

[Effects of the Invention] Based on the conventional theory, we investigated dioxin induction in animals to determine whether or not the active ingredient of soybean was related to the prevention of breast cancer, and investigated the breast cancer and prostate model experiments. An animal is fed a diet containing dioxin to induce cancer. During the induction period, mice were fed a diet containing soybean and genistein to investigate the onset of cancer. From the experiment, the incidence of cancer decreased in those who fed the diet containing genistein. Inhibited genistein-induced proliferation of breast cancer cells and prostate cells in vitro.

The incidence of cancer is compared by using an animal model for dioxin. Dioxin (DD
Feeding 20 mg / kg / day of food containing T) causes cancer after 20 weeks. When genistein (20 mg / kg / day) was added to the diet at the same time as DDT, the incidence of breast cancer was reduced to 45% when the mice were fed.

Dioxin (DBCP) in 4-week-old mice
Is injected into the abdominal cavity at 20 mg / kg / day for 30 days,
After March, mice become cancerous. When genistein (20 mg / kg / day) is included in the diet simultaneously with DBCP and the mice are fed, the incidence of breast cancer is reduced to 34%.

Dioxin (DBCP) in 4-week-old mice
Was subcutaneously injected and coadministered 6 times / week for 20 weeks. Group A mice were fed genistein (20 mg / kg / day) and group B mice were not fed. Compared to those after 32 weeks, no prostate cancer was found in group A rats, but in group B, prostate cancer occurred at a rate of 50% or more.

The pre- and post-effect effects of genistein use are determined by the proliferation of breast cancer cell (MCF-7) cultures. Genistein is a human breast cancer cell (MC
F-7) cell proliferation test (IC50 = 7-12 ug / m
In l), the growth was suppressed.

From the prostate cancer cell culture, genistein
Cell proliferation test (IC50 = 7-12u at M concentration)
(g / ml), the growth was suppressed. After using genistein, it suppressed prostate cancer cells.

Genistein and Ryoccha are lung cancer PC-9
Induction of cell apoptosis was induced. When genistein and Ryoccha were used together, synergistic results were obtained in the result of apoptotic induction.

Experiments were carried out on the increased immune function of the combination of genistein and other plants. Dioxin (TCDD) was administered to mice to conduct an animal test.
Dioxins were administered to mice at a single dose of 500 ng / kg to group A, 90 ng / kg to group B, and both groups were fed nothing but normal diet. Dioxin C group once 500ng /
Administration of kg, administration of 90 ng / kg to group D, genistein, ginseng, agarisk, and ryokcha mixed diet in addition to normal diet, and comparison of 4 months later, group AB showed thymic atrophy and decreased lymphocytes. And the immune function was suppressed.
The C group had thymic atrophy, the D group had normal thymus, the CD group had normal immune function lymphocytes.

The anti-dioxin principle of genistein is to prevent the binding of the environmental hormone and the receptor by competing with the unbound environmental hormone (dioxin) in the body and occupying the binding site of the receptor first.

Genistein is a natural plant and an angiogenesis inhibitor for cancer treatment. The function of genistein inhibits angiogenesis at the stage where cancer cells form tissues (eg breast cancer MCF-7 pathology, prostate cancer). From this, there is a possibility of developing a safer anticancer drug using genistein without side effects.

Regarding safety. Since genistein is produced from fermented soybean through steps, it is also contained in soybean fermented foods such as miso and natto, and is highly safe since it has been eaten for many years. Genistein has 72 hours of metabolic process in the human body and has no toxic effect. Hazardous dioxins have a metabolism of about 7-8 years or more. When dioxin binds to cells in the body, it is not excreted for a long time. Over time, the concentration increases and becomes carcinogenic. Genistein is expected as a health food for the prevention of dioxin carcinogenesis.

[Brief description of drawings]

Figure 1: Genistein

FIG. 2 Manufacturing process

Claims (2)

(57) [Claims] 1. High performance liquid chromatography (HPLC) from isoflavone aglycone extracted from fermented soybeans.
An anti-dioxin health food characterized by using genistein extracted in step 1 as the main ingredient. 2. An environment in which the genistein is free in the body by mixing it with agarix, aloe vera, red ginseng, ryokcha, chitin / chitosan, coix extract, garlic extract, amoto, vitamin C, dietary fiber, beta-carotene. An anti-dioxin health food characterized in that hormones and harmful substances are excreted from the body.