JP2599161B2 - Preventive and therapeutic agent for Clostridium perfringens infection in livestock and poultry - Google Patents

Description

The present invention relates to an agent for preventing and treating Clostridium perfringens infection in livestock and poultry.

More specifically, the present invention relates to a chicken comprising a crude drug as a main component,
The present invention relates to an agent for preventing and treating Clostridium perfringens infection in pigs, cattle, and sheep.

The present invention also relates to a method for preventing and treating the infectious disease comprising administering a crude drug.

[Background Art] Clostridium perfringens infection in livestock and poultry is a disease caused by infection with Clostridium perfringens, and has gangrene enteritis as a typical symptom, and also causes enterotoxemia and malignant edema. The morbidity and mortality are both high and the economic damage to the poultry, broiler, swine and dairy industries is significant. Therefore, it is one of the most important diseases for which the establishment of control measures is urgently required among a number of diseases of livestock and poultry.

Necrotizing enterocolitis is characterized by diseases such as piglets and chicks, clinically showing diarrhea, invasion of bacteria into the intestinal mucosa, and necrosis of the small intestine.

Enterotoxemia is a disease in which Clostridium perfringens proliferates in the small intestine of animals, produces necrotic, hemorrhagic lesions due to toxins produced, and causes acute death due to toxicemia.

Malignant edema is a disease that is caused by the invasion of bacteria into the wound surface created by accident or surgery, which germinates, proliferates, emits toxins, causes toxicemia, bacteremia, and death. .

Thus, despite the fact that Clostridium perfringens infection is a serious infectious disease, no preventive or therapeutic method has yet been established.

As a preventive method, breeding management is appropriately performed. However, in practice, prevention is extremely difficult.

In addition, a method of administering an antibiotic or other antibacterial agent together with feed to prevent and treat the disease has been used. However, in recent years, there is a growing tendency that it is not appropriate to administer antibiotics and the like to animals due to the occurrence of resistant bacteria and the persistence of antibiotics and the like in meat. Therefore, development of a method for preventing and treating this disease without using an antibiotic or the like and an agent for preventing and treating the disease are desired.

[Means for Solving the Problems] The present inventors have conducted studies to solve the above problems, and as a result, have found that certain crude drugs have an effect of suppressing the disease, and completed the present invention. Was.

That is, an object of the present invention is to provide an agent for preventing and treating Clostridium perfringens infection in livestock and poultry, and a method for preventing and treating the same.

The present invention relates to licorice, mugwort, spinach, orchid,
Obak, Gennoshoko, Koboku, Tanjin, Chimo, Daio, Coji, Jotei, Keikai, Keihi, Genjin, Ketsumeishi, Biwayou, Bowhu, Hop, Soyou, Iowaihi, Forsythia, Aloe, Gourd, Ireisen, Ubai, Emmaiso, Shazensou Clinch, Jinko, Senkyu, Kouhon, Gobishi, Sanshuyu, Sicon, Kouren,
Clostridium of livestock and poultry containing one or more crude drugs selected from the group consisting of peonies, bullfinch, thyme, shironanthene, chiyu and ephedra
It is intended to provide an agent for preventing and treating perfringens infection.

The present invention also provides a crude drug in an original form, an organic solvent, a mixture of an organic solvent and water or a crude drug extract obtained by extraction with water, or a preventive and therapeutic agent for the same disease in livestock and poultry containing a crude drug active ingredient. Is provided.

Further, the present invention provides liquorice, mugwort, spinach, ogre, oak, gennoshoko, kouboku, tanjin, timo, rhubarb, clove, jotei, kayai,
Keihi, Genjin, Ketsumeishi, Biwayou, Boufu,
Hop, soybean, biloba, forsythia, aloe, gourd, irisen, squid, emmeisou, shazensou, shinii, inchinko, ginko, senkyu, kohon, gobishi, sanshuyu, sicon, squirrel, peonies, quinoushi, thyme, shironanten, and shironanten It is intended to provide a method for preventing and treating the disease in livestock and poultry, comprising administering an effective amount of one or more crude drugs selected from the group consisting of:

The present invention also provides a crude drug in the form of a bulk powder, an organic solvent, a mixture of an organic solvent and water or a crude drug extract obtained by extraction with water, or a herbal medicine comprising administering the same in the form of a crude drug active ingredient. It provides a method for prevention and treatment.

The present invention also provides a method for preventing and treating the same disease in livestock and poultry, which comprises administering a crude drug in a feed.

Licorice (Glycyrrhizaglabra)
Linne 'var. Glandulifera Regel et Herder) roots and rhizomes as such or without cork skin. It contains glycyrrhizin, liquiritin, glucose, asparagine, etc. as ingredients, relieves humans, antispasmodics, It is used as

Artemisia princeps Pampa
nini) or sagebrush (A. montana Pampanini) leaves. It contains essential oils and alkaloids including α-thuyon, sesquiterpene, etc. in addition to cineol (50%) as a component, and is used for uterine bleeding and the like as an astringent and hemostatic agent for humans, and has tonic and blood-supplying effects.

Spinach (Yellow) is spinach (Coptis japonica Ma)
Fine roots are burned off from the roots of kino). It contains berberine, parimatin and the like as components, and is used as a stomach stomach bitter in humans and an intestinal medicine.

Ougon Is the root of Scutellaria baicalensis Georgi excluding the cork skin. It contains agonine and baicalin as components and is used as a human anti-inflammatory and antipyretic drug.

Yellowtail (Phellodendronamurens)
e Ruprecht) or other congeners or other species of bark without the cork layer. It contains berberine, palmatin and the like as components, and is used as a stomachic, intestinal astringent, astringent and anti-inflammatory drug for humans.

Gennoshoko, Geraniumnepale
nse Sweet). It contains tannin, gallic acid, succinic acid, etc. as components and is used as astringent drug for humans for diarrhea and abdominal pain.

Koboku (Magnolia), Magnoliaobovata Th
unberg) trees and branches. This product contains, as an ingredient, essential oils such as methylol and magnolol, essential oils and magnocoumarins, and human astringent diuresis. It is used for chest and abdominal distension, abdominal pain and asthma.

Tanjin (Salvia miltiorrhiza)
Bunge). It contains tandinone I, II and cryptotandinone as ingredients and is used for tonicity, menstruation, menstrual disorders, uterine bleeding, abdominal pain and acne as a feminine drug.

Chimo (Chimo) is a sedge (Anemanhenaasphodeloide)
s Bunge) rhizome. It contains saponin, steroid saponin, tannin and the like as components and is used as an anti-inflammatory, antipyretic, antidiarrheal, diuretic, and analgesic in humans.

Rhubarb (Rhuang) is a rhubarb (Rheumpalmatum Linn)
e ′ or R. officinale Baillon) and the relatives of the rhizomes of the rhizomes were almost completely removed and vertically divided. It contains chrysophanol, emodin, emodin monomethyl ether, etc. as anthraquinone derivatives as components, and is used for human gastric stomach bitterness, laxity, and as an anti-inflammatory drug for chronic gastrointestinal diseases, dyspepsia, and constipation. .

The butterfly (clove) is a butterfly (Eugeniacaryophylla)
ta Thunb.) just before flowering. It contains eugenol, acetyl eugenol, etc. as ingredients, promotes human digestive function, as an anthelmintic, and spices,
It is also used as cosmetics.

Jotei (Virgin) is a rat rodent (Ligustrum japoni)
cum Thunberg). This is an ingredient
Contains oleanolic acid, glucose, etc. and is used as a human tonic.

Kaigai (Negeta japonica Ma)
ximowicz) is a section of 3-5 mm long, usually in the aerial part of the flower stage. It contains d-menthon, d-limonene, and the like as components and is used as a human antiperspirant, antipyretic and antidote.

Cahi (Cinnamon bark) is Cage (Cinna momum Cassia B)
lume) is obtained by removing the dry skin and cinnamon bark and removing some of the cork layer. It has cinnamaldehyde,
It contains cinnamyl acetate and the like and is used as sweating, antipyretic, analgesic, stomachic, intestinal and anthelmintic in humans.

Genzine is a Scrophularia O
ldhami Oliver). It is used as an anti-inflammatory, antipyretic, analgesic and venereal agent in humans.

Ketsumeishi (Keishiko) is a Japanese lobster (Cassiaobtusi)
folia Linne '). It contains emodin, obtusifuolin and the like as components, and is commonly called hub tea and is substituted for tea as a beverage.

Loquat (loquat leaf) is loquat (Eriobotrya japonica)
Lindley) leaves are removed as much as possible.
It contains glucose, sucrose, fructose, maltose, and the like as components, and has been used as a cool and stomach medicine for humans and as a preventive for hot weather.

Bofu (Windbreak) is Ibukibofu (Seselilibano)
tis Koch var. daucifolia DC.) together with rhizomes. It contains essential oil as an ingredient, and is used as a sweating, antipyretic and analgesic in humans with headache, chills and joint pain due to colds.

Hops are mature ears of hops (Humulus Lupulus Linne '). It contains tannin, humulone, lupulon and the like as components and is necessary for imparting bitterness and aroma in beer brewing.

Soybean (Soyo) is perilla (Perilla frutescens Britt)
on var.acuta Kudo). It contains 0.5-1% essential oil as a component, 55% of which is perilaldehyde, and also contains limonene, α-pinene and the like. It is used as excitable sweating, antipyretic, coughing, analgesic, sedative, diuretic, stomachic and the like in humans.

Bayberry (fried plum skin), bayberry (Myricarubra Si
ebold et Zuccarini). It contains tannin, myricetin, myricitrin, etc. as components, is used as astringent drug for humans for diarrhea, and is also effective for bruises.

Forsythia (Forsythia susp)
ensa Vahl) or Datura (F.koreanum)
Ohwi) fruit. It contains oleanolic acid as a component and is used as a detoxification, drainage, anti-inflammatory and diuretic in humans.

Aloe is aloe (Aloe arborescens Mill.var.nata
lensis Bergel.) It contains barbaloin, aloe-emodin, etc. as components and has been used as a human bitter stomachic or laxative for a long time in the Middle East and Europe.

Gyutan (cow bile) is a cow (Bostaurusdomesticus)
Gmelin) can be obtained by hanging the gall bladder in the shade or by incising the bile duct and storing the bile in a sealed container. It contains, as an ingredient, sodium cholate, mucoprotein, fatty acids and the like, and has been used for a long time for the treatment of human jaundice, constipation, diabetes, hemorrhoids and the like.

Irene (Celeme)
owicziana Franchet et Savatier var.forida Thunber
g) or a root of a closely related plant of the same genus. It contains saponin as an ingredient and is used for gout, rheumatism and neuralgia as a human diuretic and analgesic.

Ubai (Plumus Mume Siebold et Z)
The fruit is peeled from the immature fruit of uccarini), buried in soot and dried. The ingredients for this are succinic acid,
Citric acid, malic acid, tartaric acid and the like are used as a refreshing astringent for humans, antidiarrheal, exterminating Ascaris, and antipyretic.

Enmeiso (extended lifegrass) is a cypress (Isodon jap)
onica Hara) or black clover (Isodon trich)
ocarpus Kudo). This is an ingredient
It contains Plectrantin and Emmain, and is used as a stomach bitter medicine in humans for the treatment of indigestion, anorexia, abdominal pain and the like.

Shazensou (vegetative grass) is a psyllium (Plantage asi
atica Linne '). It contains, as an ingredient, aukbin, plantaginine, etc.
It is used as a diuretic for human eye diseases, cystitis and the like.

Shin Yi, Kobushi (Magnolia Kobus APDe)
It is the bud of Candolle). It contains citral, eugenol, cineol and the like as ingredients and is used as a fragrance for human headache, venom and nasal diseases.

Clapperboard The Artemisia capillaris Thunberg
This is the spike. This product contains terpene compounds such as capylene, β-pipen, capilone, and capillin as well as capillarin in the incmarine body.
It is effective as a diuretic for febrile jaundice.

Jingko (Agaric) is a Jingko (Aquilariaagallocha)
Roxb.) Containing wood. This material contains benzylacetone, p-methoxybenzylacetone, hydrocinnamic acid, and the like as components, and is a material that plays the main role of "Kodo" as a perfume. As a medicament, it is used for asthma, vomiting, abdominal pain, etc., with the expectation of a kind of sedative action and fatigue recovery action.

Sengkyu Is a rhizome excluding the root of Cecium officinale Makino, which is usually blanched and dried. It contains kunidinium lactone, kunidiic acid and the like as components and is used as a blood purifying, blood-supplying, tonic and sedative for humans such as anemia, chills and irregular menstruation.

Kouhon (straw) is a crustacean (Nothosmyrnium japo
niccum Miquel) rhizome. It contains essential oils containing notosmyrnol and dimethoxyallylbenzene as main components, and is used as an antispasmodic and analgesic for humans.

Gobishi is a goal mainly formed on the leaves of nurde (Rhus javanica Linne ') due to a stab wound of a nuldenomifushibashimushi (Melaphis chinensis J. Bell). Including. It is used as a raw material for producing quintuple tincture, tannic acid, gallic acid, and pyrogallol.

Sanshuyu (Sanshuyu), Sanshuyu (Cornus offic)
inalis Siebold et Zuccarini). It contains crystalline organic acids, gallic acid, malic acid, tartaric acid and the like as components and is used as a nourishing, tonic and astringent drug for humans.

Shikon (purple root) is purple (Lithospermum officin)
ale Linne 'var.erythrorhizon Maximowicz). It contains a purple pigment acetyl shikonin as a component and is used as a detoxifying and detoxifying agent for humans.

Kouren (Hou Lian) is the cricket (Picrorrhiza)
It is the rhizome of Kurooa Royle ex Benth.). It contains cutin, D-mannitol, vanillic acid and the like as components and is used as a human bitter stomach and laxative.

Peony (peony) is a peony (Paeonia albifl)
ora Pallas var.trichocarpa Bunge). Contains benzoic acid, resin, etc. as ingredients, astringent, relaxing,
It is used as an analgesic and antispasmodic.

Kinu Oshi (Kanekurako) is a Nani Waibara (Rosa Laevi)
gata Michaux). This is an ingredient
Citric acid, malic acid, tannin, saponin, vitamin C
It is used to stop human diarrhea and polyuria.

Thyme is Thymus vulgaris Linn
e ′) It is the whole plant in the flowering season. It contains thymol, parasimole, pinene and the like as components, and its decoction or extract is used as a human antitussive. It is also used in foods as a flavoring agent that also serves as a preservative.

Shironanthen is Nandina domestica Thun
b.var.leucocarpa Makino fruit is dried. It contains domestine, protopin (alkaloid) and the like as components and is used as an antitussive for human asthma, pertussis and the like.

Chiyu (Jiyu) is a waremokou (Sanguisorba officina)
lis L.) and dried. It contains tannin (17%) and saponin as components, and is used as a hemostatic astringent in humans for hematemesis, hemoptysis, and menorrhagia.

Ephedra (Ephedra) is Ephedra (Ephedra sinica Stapf)
Above ground stem. It contains l-ephedrine, d-pseudoephedrine and the like as components and is used as an antitussive, sweating and diuretic in humans.

As described above, the crude drugs used in the present invention, liquorice, mugwort, spinach, giant gourd, oubaku, genoshoko, kouboku, tanjin, timo, rhubarb, clove,
Jotei, Keigai, Keihi, Genjin, Ketsumeishi,
Loquat, boufu, hop, soybean, lobster, forsythia, aloe, gourd, irisen, ubai,
Emmeso, Shazenso, Shiny, Inchinko,
Each of Jinko, Senkyu, Kohon, Gobishi, Sanshuyu, Sikon, Koouren, Peony, Kinuushi, Thyme, Shironanten, Chiyu and Ephedra, each alone or in combination, has been used for the prevention and treatment of human diseases. However, no attempt has been made to use them for the purpose of preventing and treating animal diseases, in particular, for preventing and treating animal infectious diseases.

The present inventors have conducted various studies focusing on the medicinal effects of the crude drugs described above, and as a result, described above in the prevention and treatment of Clostridium perfringens infection, that is, a disease caused by infection of livestock and poultry with Clostridium perfringens. The present inventors have found that it is extremely effective to administer one or more crude drugs in combination, and have completed the present invention.

That is, as described below, the above-mentioned specific types of crude drugs and their extracts can
Clostridium perfringens was found to have a specific bacteriostatic and bactericidal action. And this Clos
As a result of the inhibitory effect on tridium perfringens, Clostridium perfringens
Thus, it was possible to prevent and cure clostridial infection, which is a disease of livestock and poultry originating from the country.

The fact that these specific herbal medicines and extracts thereof are effective against the diseases specific to livestock and poultry was unexpected and was first revealed by the present inventors.

These crude drugs may be directly administered as raw powder or in the form of an extract or crude drug active ingredient, or they may be added to feed or in the form of a solution, dispersion, semi-solid, powder, granule, or molded product. , Leaching preparations, injection preparations and the like.

The extraction extract is used, for example, by solvent extraction using water, an organic solvent or a mixture of water and an organic solvent, and then directly extracting, concentrating, diluting, or removing the solvent.

As the organic solvent, for example, methanol, ethanol,
n-Propanol, n-butanol, acetone, ethyl acetate, ether, methylene chloride, chloroform, benzene, carbon tetrachloride, petroleum ether and the like are used, and methanol and ethanol are particularly desirable. These organic solvents are 1
It can be used as a species or a mixture of two or more species.

This solvent extraction can be performed by cold or hot digestion of the crude drug in these solvents. 15 ℃ ~ 25 for cold immersion
C. and in the case of digestion, it can be carried out in a temperature range of 35 to 45.degree. The extraction time depends on the extraction temperature, but is generally about 5 days or until the soluble components are sufficiently dissolved.

Crude drug active ingredient, for example, the extract extract is further shaken using various solvents, a fraction that is transferred to this solvent is extracted, and the solvent is distilled off, and the effective fraction, that is, the active ingredient may be used as the active ingredient. it can.

Solvents used in this case are non-polar solvents such as lower aliphatic ethers (diethyl ether, ethyl methyl ether, etc.); lower halogen alkanes (chloroform, dichloroethane, dibromoethane, etc.); benzenes (benzene, toluene , Xylene, etc.); and other petroleum solvents (petroleum ether, petroleum benzene, ligroin, etc.).

The remaining aqueous solution obtained by extracting the effective fraction is shake-extracted by using water and a certain organic solvent saturated with water, washed with water, and transferred to an organic solvent layer as a residue. The solvent obtained by distilling off the solvent of the fraction to be used can also be used as an effective fraction, that is, an active ingredient. This effective fraction differs from the above-mentioned effective fraction in the physicochemical properties and the detected components are also different.

The dose is 0.001 mg / kg of body weight in terms of the weight of the crude drug.
/ Day to 100 g / kg body weight / day. This amount is different when used as a prophylactic and therapeutic agent,
Generally, the latter dose will be higher.

The method of administration can be oral or parenteral, and parenteral administration can be intramuscular, intraperitoneal, cinnamon, nasal, intravenous, and the like.

[Effects of the Invention] As described in detail above, the present invention provides an effective agent for preventing and treating Clostridium perfringens infection and an effective method for preventing and treating the disease.

Since the prophylactic and therapeutic agent of the present invention is mainly composed of a crude drug or an extract obtained therefrom, there is no problem of the occurrence of resistant bacteria or side effects in the case of antibiotics and the like, and the agent can be administered with confidence.

Moreover, even if the animal is a meat animal, an animal that produces eggs and milk, there is no concern about the effect of the residual drug on the human body.

Furthermore, the disease can be prevented and treated at a lower cost than when an antibiotic or the like is used.

Hereinafter, the present invention will be described in more detail with reference to Test Examples and Examples.

Test Example 1 The sensitivity of Clostridium perfringens (bird-derived and pig-derived) to various crude drugs was examined.

Various crude drugs were added to a 50% aqueous ethanol solution 7 times the amount of the crude drug and extracted for 16 hours to obtain an alcohol extract.

The extract was infiltrated into an 8 mm diameter disk by 25 μl and dried. Insert this disc into Clostridium perfringens
(Bird-derived and pig-derived) strains were placed on a Mueller-Hinton agar medium, cultured anaerobically at 37 ° C. for 18 hours, and the diameter of the inhibition circle was measured.

 Next, Table 1 shows the test results.

Example 1 Basic Feed Corn 450 parts by weight Micro 260 parts by weight Soybean meal 190 parts by weight Fish meal 50 parts by weight Yellow grease 30 parts by weight Calcium carbonate 7 parts by weight Calcium tertiary phosphate 6 parts by weight Salt 2 parts by weight Pre Mix 5 parts by weight A test feed was prepared by adding 0.5% by weight of a powder of uren to the above basic feed.

The test feed was freely fed to 760 healthy 28-day-old chickens. The number of chickens that died from field infection with Clostridium perfringens for 35 days from the start of the test was observed. In the control group, a basal feed was given instead of the test feed.

As a result, the number of chickens that died from the Clostridium perfringens field infection was 2 in the test plot of the present invention, while the number of chickens in the control plot was 58.

Examples 2-42 The same procedures as in Example 1 were carried out except that the crude drugs shown in Table 2 below were added in an amount of 0.6% in place of the urethane.

 Next, the test results are shown in Table 2.

Example 44 Licorice, mugwort, spinach, ogre, oak,
Gennoshoko, Koboku, Tanjin, Chimo, Daioh, Coji, Jotei, Keigai, Keihi, Genjin, Ketsumeishi, Biwayou, Bohu, Hop, Soyoo, Yojibaig, Forsythia, Aloe, Gyotan, Iraisen, Ubai, Emmaiso, Shazensou, Shinyin, Shinyin Ginko, senkyu, kohon,
Take 1 kg each of Gobishi, Sanshuyu, Sicon, Kourouen, Peony, Kinuushi, Thyme, Shironanten, Chiyu and Ephedra, put them in 10 pieces of water, 100 ° C
For 1 hour. Each water extract was diluted 1000 times to obtain an extract.

Using 485 healthy chickens aged 28 days, the extract was fed instead of water. As the feed, the same basic feed as that used in Example 1 was used. The control group was given water instead of the extract. In this way, the animals were reared for 35 days from the start of the test, and the number of dead birds due to Clostridium perfringens field infection at that time was observed. As a result, the number of dead birds due to Clostridium perfringens infection was smaller in all the test plots than in the control plot.

Example 45 Basic feed Corn 450 parts by weight Microscopically 310 parts by weight Soybean meal 146 parts by weight Seaweed meal 30 parts by weight Fish meal 15 parts by weight Meat and bone powder 10 parts by weight Yellow grease 18 parts by weight Calcium carbonate 5 parts by weight Phosphoric acid 8 parts by weight of calcium 3 parts by weight of salt 3 parts by weight of premix 5% by weight of the above-mentioned basic feed was added with 0.5% by weight of a powder of uren to prepare a test feed. Use this test feed as a 30-day-old healthy pig
Fifty animals were fed freely and kept for 60 days.

 In the control group, a basal feed was given instead of the test feed.

Then, the number of pigs that died from the Clostridium perfringens field infection during the test period and the total number of diarrhea days due to the Clostridium perfringens field infection were observed. As a result, the number of pigs that died due to Clostridium perfringens field infection was 0 in the test plot of the present invention, whereas it was 7 in the control plot. The total number of pigs with diarrhea during the test period was 5/300 in the test plot.
The number of animals was 0, whereas that of the control group was 111/3000.

Examples 46 to 87 The procedures were the same as in Example 45, except that the crude drug shown in Table 3 below was added to the basal feed in an amount of 0.6% in place of the powdered urine.

 Next, the test results are shown in Table 3.

Example 88 Licorice, mugwort, orange, ogre, oak,
Gennoshoko, Koboku, Tanjin, Chimo, Daioh, Coji, Jotei, Keigai, Keihi, Genjin, Ketsumeishi, Biwayou, Bohu, Hop, Soyoo, Yojibaig, Forsythia, Aloe, Gyotan, Iraisen, Ubai, Emmaiso, Shazensou, Shinyin, Shinyin Ginko, senkyu, kohon,
Take 1 kg each of Gobishi, Sanshuyu, Sicon, Kourouen, Peony, Kinuushi, Thyme, Shironanten, Chiyu and Ephedra, put them in 10 pieces of water, 100 ° C
For 1 hour. Dilute each water extract 1000 times,
The extract was used.

50 pigs, 30 days old, were used in one group, and the test group was provided with the extract instead of water, and the control group was provided with water. As the feed, the same basic feed as that used in Example 45 was used.
Clostridium bred for 60 days in this manner
The number of deaths and the number of diarrhea due to Perfringens field infection were observed.

As a result, the number of dead animals and the number of diarrhea in all the test plots were smaller than those in the control plots.

──────────────────────────────────────────────────の Continuation of the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical display location A61K 35/78 A61K 35/78 KENVFHB AEV AEVT (56) References JP-A Sho 61-210038 (JP, A) JP-A-48-58115 (JP, A) JP-A-51-61612 (JP, A) JP-A-62-259214 (JP, A) JP-A-62-12720 (JP, A A) JP-A-57-26549 (JP, A) JP-A-57-132847 (JP, A) Kanayoshi Akamatsu, Shinshin Wakan Yakuhin, Medical and Dental Medicine Publishing Co., Ltd., October 15, 1980 , 1st edition, 5th printing, 327, 1, 8, 11, 454, 110, 301, 314, 433, 109, 589, 493, 209, 160, 103, 421, 92, 350, 358, 196, 510,105,529,155,784,461,369,97,79,432,5,218,189,197,268,178,120,90,457,381,114,442,38 See page 6,655

Claims (5)

(57) [Claims] Claims: 1. A liquorice, mugwort, spinach, ogre,
Obak, Gennoshoko, Koboku, Tanjin, Chimo, Rhubarb, Coji, Jotei, Keigai, Keihi, Genjin, Ketsumeishi, Biwayou, Bowhu, Hop, Soyou, Yowaihi, Forsythia, Aloe, Gyutan, Ireisen, Ubai, Emmaiso, Shazensou Clinch, Jinko, Senkyu, Kouhon, Gobishi, Sanshuyu, Sicon, Kouren,
Clostridium of livestock and poultry containing one or more crude drugs selected from the group consisting of peonies, bullfinch, thyme, shironanten, chiyu and ephedra
Agent for preventing and treating Perfringens infection. 2. The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is in bulk form. 3. The preventive and therapeutic agent according to claim 1, wherein the crude drug is an organic solvent, a mixture of water and an organic solvent, or a crude drug extract extracted with water and / or a crude drug active ingredient obtained therefrom. . 4. The preventive and therapeutic agent according to claim 1, wherein the crude drug is incorporated in feed. 5. The preventive and therapeutic agent according to claim 1, wherein the livestock and poultry are chickens, pigs, cows and sheep.