JP2024019244A - Liquid storage containers, liquid storage container sets, and sterile bonding kits - Google Patents

Abstract

To provide a liquid storage container that allows the length of a liquid delivery pipe formed by a sterile connection device to be made constant.SOLUTION: A liquid storage container 1 comprises: a container body 20 defining a liquid storage space 22; a first tubular member 40 whose one end 40a is connected to the container body 20; and a first closing part 48 for closing the first tubular member 40, the first tubular member 40 having an inner surface 44 that defines together with the first closing part 48 a flow path 42 that communicates with the storage space 22, the first tubular member 40 comprising at least a first mark part 46a and a second mark part 46b, which are disposed at mutually different positions between the one end 40a of the first tubular member 40 and the first closing part 48 in the extending direction of the first tubular member 40.SELECTED DRAWING: Figure 1

Description

The present invention relates to a liquid container, a liquid container set, and a sterile bonding kit.

Liquids such as cell suspensions, pharmaceutical liquids, and food-related liquids must be protected from contamination by foreign substances, bacteria, and the like. Therefore, when transferring such a liquid from one container to another, it is necessary to connect the containers and transfer the liquid while keeping the insides of these containers closed from the outside environment. There is a need to do. In such cases, a sterile bonding device such as that described in Patent Document 1 can be used. The aseptic joining device described in Patent Document 1 fuses a first tube and a second tube with a heating member in a state where these tubes are overlapped and fixed, and then connects the melted end of the first tube with the second tube. This is a device that replaces and joins the fused ends of tubes. By joining the tubes extending from two storage containers using such a sterile joining device, it becomes possible to transfer liquid between the storage containers via the liquid delivery pipe formed by the joined tubes.

Patent No. 6197025

When moving a liquid from one storage container to another storage container via a liquid transfer pipe, liquid may remain inside the liquid transfer pipe. Depending on the amount of liquid remaining in the liquid sending pipe, the amount of liquid flowing into the other container also changes. Generally, the amount of liquid remaining in the liquid pipe increases as the liquid pipe becomes longer. Therefore, in order to keep the amount of liquid flowing into the storage container constant, it is desirable to keep the length of the liquid feeding pipe constant. Furthermore, the length of the liquid feeding tube also affects the ease with which operations such as liquid feeding can be performed.
Therefore, from the viewpoint of workability, it is desirable to keep the length of the liquid pipe constant.

However, when forming a liquid supply pipe between storage containers using an aseptic joining device as described in Patent Document 1, at which position of the first tube and second tube to be joined is the operator installing the aseptic joining device. The position where the first tube and the second tube are melted and joined varies depending on the
As a result, the length of the liquid pipe to be formed also varies.

The present invention has been made in view of these points, and an object of the present invention is to provide a liquid storage container in which the length of a liquid feeding tube formed by a sterile joining device can be made constant.

A liquid storage container according to one aspect of the present invention includes a container main body that defines a liquid storage space, a first tubular member whose one end is connected to the container main body, and a first tubular member that closes the first tubular member. 1 closure part, the first tubular member has an inner surface that defines, together with the first closure part, a flow path communicating with the accommodation space, and the first tubular member has a At least a first mark part and a second mark part are arranged at mutually different positions between the one end of the first tubular member and the first closing part in the extending direction of the first tubular member. .

In the liquid storage container of the present invention, the length of the liquid feeding tube formed by the aseptic bonding device can be made constant.

FIG. 1 is a schematic cross-sectional view including the longitudinal axis of a first tubular member, showing an example of a liquid storage container according to an embodiment. FIG. 2 is a schematic cross-sectional view including the longitudinal axis of the first tubular member, showing an example of the liquid storage container according to the embodiment. FIG. 3 is a schematic diagram showing an example of a cross section of the first tubular member taken along line AA in FIG. FIG. 4 is a schematic diagram showing an example of a cross section of the first tubular member taken along line BB in FIG. FIG. 5 is a schematic diagram showing another example of the cross section of the first tubular member taken along line BB in FIG. 1. FIG. 6 is a schematic cross-sectional view including the longitudinal axis of the first tubular member, showing an example of the liquid storage container according to the embodiment. FIG. 7 is a schematic cross-sectional view including the longitudinal axis of the first tubular member, showing an example of the liquid storage container according to the embodiment. FIG. 8 is a schematic cross-sectional view including the longitudinal axes of the first tubular member and the second tubular member, showing an example of the liquid storage container set according to the embodiment. FIG. 9 is a schematic diagram showing an example of a cross section of the mark portion of the first tubular member and the mark portion of the second tubular member that are in plane contact with each other, taken along line CC in FIG. 8. FIG. 10 is a schematic diagram showing an example of a cross section of the mark portion of the first tubular member and the mark portion of the second tubular member that are in plane contact with each other, taken along line CC in FIG. 8. FIG. 11 is a schematic diagram showing an example of a cross section of the mark portion of the first tubular member and the mark portion of the second tubular member that fit into each other, taken along line CC in FIG. 8. FIG. 12 is a schematic cross-sectional view including the longitudinal axes of the first tubular member and the second tubular member, showing an example of the marking portion of the first tubular member and the marking portion of the second tubular member that fit into each other; be. FIG. 13 is a schematic diagram showing an example of a cross section of the mark portion of the first tubular member and the mark portion of the second tubular member taken along line EE in FIG. 12. FIG. 14 is a schematic cross-sectional view including the longitudinal axes of the first tubular member and the second tubular member, showing an example of the first tubular member and the second tubular member installed in the sterile bonding device. FIG. 15 is a schematic cross-sectional view including the longitudinal axis of the liquid pipe, showing an example of a liquid pipe formed by joining the first tubular member and the second tubular member using a sterile joining device.

(1) Liquid Container First, the liquid container according to the embodiment will be described. As shown in FIG. 1, the liquid storage container 1 according to the embodiment includes a container body 20 defining a liquid storage space 22, a first tubular member 40 whose one end 40a is connected to the container body 20, and a first tubular member 40 whose one end 40a is connected to the container body 20. A first closing portion 48 that closes one tubular member 40 is provided. The first tubular member 40 has an inner surface 44 that defines, with a first closure 48 , a flow path 42 that communicates with the receiving space 22 . The first tubular members 40 are arranged at mutually different positions between the one end 40a of the first tubular member 40 and the first closure part 48 in the extending direction (i.e., longitudinal axis direction) of the first tubular member 40. In addition, two mark portions 46a, 46b (
A first mark part 46a and a second mark part 46b) are provided.

The container body 20 may have any structure and may be formed from any material as long as it defines therein a storage space 22 for storing liquid in a sterile manner. For example, as the container body 20, a soft bag processed from a film, a molded container by blow molding, injection molding, etc., a glass container, etc. can be used. The container body 20 is preferably made of a material that is transparent enough to confirm the presence or absence of liquid in the storage space 22. For example, it may be formed from polyolefin resin such as polyethylene or polypropylene, polyvinyl chloride, ethylene vinyl acetate copolymer resin, C-FLEX (registered trademark), polystyrene, glass, or the like.
Further, the material of the container body 20 may be the same as that of the first tubular member 40, or may be a material that can be welded to the first tubular member 40. Thereby, the container main body 20 and the first tubular member 40 can be connected without using an adhesive, so that contamination due to the adhesive can be avoided.

The container body 20 may be, for example, a cell storage container, a culture solution storage container, a culture container, or a waste liquid container. The accommodation space 22 defined in the container body 20 is formed through an opening 24 formed in the container body 20.
It communicates with a flow path 42 in the first tubular member 40 via. Further, the container body 20 may have an injection port (not shown) for injecting liquid into the accommodation space 22. The injection port is located in the accommodation space 22
The opening may be any opening that can be closed after injecting liquid into the opening.

The first tubular member 40 has a cylindrical shape with an inner surface 44 and an outer surface 45. 1st
The tubular member 40 has an opening 47 at one end 40a. The first tubular member 40 is connected to the container body 20 such that the opening 47 of the first tubular member 40 and the opening 24 of the container body 20 communicate with each other.

The first closing portion (closure portion) 48 is not particularly limited as long as it closes (occlusions) the first tubular member 40 . Examples of the first closing portion 48 include a welded portion formed by crushing and welding the first tubular member 40 in the radial direction, and a plug (for example, a rubber plug) filled in the first tubular member 40.
, a film adhered to the other end 40b of the first tubular member 40 by a top seal, a cap attached to the other end 40b of the first tubular member 40, and a wall surface integrally formed with the first tubular member 40.

The first closure portion 48 may be provided at the other end 40b of the first tubular member 40, as shown in FIG. Alternatively, as shown in FIG. 2, one end 40a and the other end 40b of the first tubular member 40
It may be provided between. When the first closing portion 48 is provided at the other end 40b of the first tubular member 40, dust etc. are less likely to accumulate at the other end 40b. When the first closing part 48 is provided between the one end 40a and the other end 40b of the first tubular member 40, since the first closing part 48 is located inside the first tubular member 40, the first closing part 48 is located inside the first tubular member 40. The risk of the first tubular member 40 being opened due to damage to the portion 48 can be reduced.

The inner surface 44 of the first tubular member 40 and the first closure 48 together define a flow path 42 that communicates with the receiving space 22 . The accommodation space 22 and the flow path 42 form one closed space. In this application, a "closed space" refers to a space that is closed to the outside environment to prevent contamination by foreign substances, bacteria, etc. in the outside environment, such as a closed space that is sealed from the outside environment. means.

Although the first tubular member 40 in FIG. 1 has a substantially columnar shape extending in one direction, the first tubular member 40 is not limited thereto, and defines a liquid flow path 42 and is a sterile bonding device. It may have any shape that can be joined. For example, first tubular member 40 may be curved or branched. Furthermore, the first tubular member 40 may be flexible. The first tubular member 40 may have a length of 10 cm or more in the stretching direction from the viewpoint of facilitating the liquid feeding operation. The first tubular member 40 can be manufactured by extrusion molding, injection molding, blow molding, or the like.

FIG. 3 shows a cross section of the first tubular member 40 taken along line AA in FIG. An example of a cross section of the part is shown. In this example, both the inner surface 44 and outer surface 45 of the first tubular member 40 have circular cross-sectional shapes. However, the inner surface 44 and outer surface 4 of the first tubular member 40
The cross-sectional shape of 5 is not limited to a circle, but may be any shape such as an ellipse or a polygon.

The first tubular member 40 is made of any material that can be joined using a sterile joining device, which will be described later.
For example, the first tubular member 40 may be formed from a heat-weldable synthetic resin. Further, the first tubular member 40 may have such transparency that the interface between the liquid and air in the flow path 42 can be visually recognized from the outside. Thereby, the presence or absence of liquid in the flow path 42 can be determined. Specifically, examples of the material of the first tubular member 40 include polyvinyl chloride, C-FLEX (registered trademark), ethylene vinyl acetate copolymer resin, polyolefin such as polyethylene, and polypropylene.

The first tubular member 40 has two mark portions 46a and 46b. Two mark parts 46a, 4
6b is connected to one end 40a of the first tubular member 40 in the extending direction of the first tubular member 40.
are arranged at different positions between the closing portions 48 of , separated by a predetermined distance d. The predetermined distance d between the two landmarks 46a and 46b corresponds to the width D (see FIG. 14) of the holder 80 of the sterile bonding device, which will be described later.

The marks formed on the mark parts 46a and 46b have any form that is visible. In the example shown in FIG. 1, the marks are formed by making the outer surface 45 of the first tubular member 40 convex at the mark parts 46a and 46b. At the mark portions 46a, 46b, the first tubular member 4
A groove (recess) serving as a mark may be formed on the outer surface 45 of the 0. Mark parts 46a, 46b
There may also be direct printing, laser printing, or other printing. The mark portions 46a, 46b may be colored. A binding band or the like may be wrapped around the first tubular member 40 as a mark at the mark portions 46a, 46b. The marks formed on the two mark parts may be separate or may be connected and integrated.

4 and 5 show a cross section of the first tubular member 40 taken along the line BB in FIG. An example of a cross section is shown below.

In the example shown in FIG. 4, both the inner surface 44 and outer surface 45 of the first tubular member 40 are
It has a circular cross-sectional shape. Outer surface 4 of first tubular member 40 at marking portions 46a, 46b
5 is the diameter of the outer surface 45 of the first tubular member 40 in the portions other than the marking portions 46a and 46b.
larger than the diameter of As a result, the outer surface 45 of the mark portions 46a and 46b becomes convex, forming a mark. Inner surface 44 of first tubular member 40 at marking portions 46a, 46b
is equal to the diameter of the inner surface 44 of the first tubular member 40 at the portions other than the marking portions 46a and 46b.

In the example shown in FIG. 5, the inner surface 44 of the first tubular member 40 has a circular cross-sectional shape and the outer surface 45 has a triangular cross-sectional shape. First tubular member 4 in marking portions 46a, 46b
The shape of the outer surface 45 of
The shape of the outer surface 45 is different from that of the outer surface 45 of As a result, the outer surface 45 of the mark portions 46a and 46b becomes convex, forming a mark. First tubular member 4 in marking portions 46a, 46b
The diameter of the inner surface 44 of
is equal to the diameter of the inner surface 44 of.

In the example shown in FIGS. 4 and 5, the first tubular member 40 perpendicular to the extending direction of the first tubular member 40
The cross-sectional outline of the mark parts 46a, 46b is different from the cross-sectional outline of the portion of the first tubular member 40 other than the mark parts 46a, 46b perpendicular to the extending direction of the first tubular member 40. As a result, the outer surface 45 of the mark portions 46a and 46b becomes convex, forming a mark. Here, "different external shapes" means that the external shapes and/or dimensions are different. Note that the external shape of the cross section of the mark portions 46a, 46b of the first tubular member 40 perpendicular to the stretching direction of the first tubular member 40 is not limited to the circular shape or triangular shape as shown in FIGS. 4 and 5. , a rectangular shape, a polygonal shape, or any other arbitrary shape. Further, as described later, the mark portions 46a and 46b are
The first tubular member 4 may have a holder fitting portion that fits into the holder of the sterile bonding device.
It may have a shape that makes surface contact with or fits with the mark part of the second tubular member that is joined to the second tubular member.

One of the mark parts 46a, 46b (first mark part 46a) is different from the other (second mark part 46b)
It may have a different shape. For example, the first mark part 46a has a different external shape from the second mark part 46b in a cross section perpendicular to the stretching direction of the first tubular member 40 and/or in a cross section parallel to the stretching direction of the first tubular member. may have. Moreover, among the two mark parts 46a and 46b,
The first marking portion 46a, which is closer to the one end 40a of the first tubular member 40, may have a smaller profile than the second marking portion 46b, which is closer to the first closure portion 48. In this case, as will be described later, the liquid feeding tube obtained by joining the first tubular member 40 to the second tubular member includes a first marking portion 46a with a small external shape, but a second marking portion 46a with a large external shape. It does not include the mark portion 46b. Therefore, the workability of the liquid transfer pipe is improved. Moreover, the first mark part 46a and the second mark part 46b may be colored in different colors.

In the example shown in FIGS. 1 to 5, the first tubular member 40 perpendicular to the extending direction of the first tubular member 40
In the cross section of the mark portions 46a, 46b, the shape and dimensions of the inner surface 44 are perpendicular to the extending direction of the first tubular member 40, in a cross section of a portion of the first tubular member 40 other than the mark portions 46a, 46b, The shape and dimensions of the inner surface 44 are the same. Thereby, the flow of liquid in the flow path 42 is stabilized, and generation of pulsating flow can be avoided. Note that "having the same shape and dimensions" means that there is no difference in shape and dimensions that would affect the flow of liquid within the flow path 42, and it does not necessarily mean that the shapes and dimensions are completely the same. good.

FIG. 6 shows another example of the liquid storage container 1 according to the embodiment. In the example shown in FIG. 6, the diameter of the flow path 42 between the first marking part 46a and the first closing part 48 and in the vicinity of the first marking part 46a (that is, in the extending direction of the first tubular member 40 diameter of inner surface 44 in vertical section)
is smaller than the diameter of the flow path 42 in other parts. Such a first tubular member 40
When joining a second tubular member (described later) to form a liquid feeding tube, since the volume of the flow path 42 in the discarded portion (the portion that does not constitute the liquid feeding tube) of the first tubular member 40 is small, the waste Less liquid is discarded with the parts.

FIG. 7 shows still another example of the liquid storage container 1 according to the embodiment. In the example shown in Figure 7,
The diameter of the flow path 42 between the first mark part 46a and the first closing part 48 and in the vicinity of the first mark part 46a is larger than the diameter of the flow path 42 in other parts. When forming a liquid feeding tube by joining such a first tubular member 40 to a second tubular member to be described later, since the area of the joint surface between the first tubular member 40 and the second tubular member is large, It becomes possible to reliably join with high joint strength.

Note that the first tubular member 40 may be provided with a further mark part (not shown) in addition to the two mark parts 46a and 46b. That is, the first tubular member 40 has at least two
The mark portions 46a and 46b are provided. The further marking portions may have similar shapes and dimensions to the two marking portions 46a, 46b, or may have different shapes and dimensions.

(2) Liquid Container Set Next, a liquid container set according to the embodiment will be described. As shown in FIG. 8, the liquid storage container set 2 according to the embodiment includes the liquid storage container 1 according to the embodiment described above, a second tubular member 60, and a second closure for closing the second tubular member 60. A member 3 comprising a portion 68a and a third closing portion 68b. The second tubular member 60 has a second closing portion 68a and a third closing portion 68a.
has an inner surface 64 defining a flow passageway 62 between the closure portions 68b. The second tubular member 60 is
Two mark parts 66a and 66b (third mark part 66a and A fourth mark portion 66b) is provided. The distance between the two marking parts 66a and 66b of the second tubular member is the distance d between the two marking parts 46a and 46b of the first tubular member 40 of the liquid storage container 1.
and corresponds to the width D (see FIG. 14) of the holder 80 of the aseptic bonding device described later.

The shape and material of the second tubular member 60 are the same as those of the first tubular member 40 described above, so a description thereof will be omitted. The second tubular member 60 preferably has the same shape as the first tubular member 40 and is made of the same material as the first tubular member 40 . In addition, two mark portions 66a, 66
The diameters of the inner and outer surfaces of the second tubular member between b
The diameters of the inner and outer surfaces of the first tubular member 40 between 6a and 46b may be the same.
Thereby, the first tubular member 40 and the second tubular member 60 can be reliably joined.

The second closing portion 68a and the third closing portion 68b may each be any member. For example, similar to the first closure part 48 of the liquid storage container 1, it may be a weld, a stopper, a film, a cap, or a wall surface that closes the second tubular member 60. Alternatively, the second closure part 68a and/or the third closure part 68b may be a container body similar to the container body 20 of the liquid storage container 1, which is connected to one end of the second tubular member 60. Alternatively, a liquid feeding mechanism such as a peristaltic pump, a syringe pump, a diaphragm pump, or a pressure pump connected to one end of the second tubular member 60 may be used. The inner surface 64 of the second tubular member 60, the second closure 68a, and the third closure 68b together define a liquid flow path 62. The flow path 62 forms one closed space.

The second closing portion 68a and the third closing portion 68b may be provided at both ends of the second tubular member 60, or may be provided between both ends of the second tubular member 60. Second closure 68
One of the closing portion a and the third closing portion 68b may be provided at the end of the second tubular member 60, and the other may be provided between both ends of the second tubular member 60. Dust and the like are less likely to accumulate at the ends where the second closing portion 68a and/or the third closing portion 68b are provided. The second closing portion 68a and/or the third closing portion 68b provided between the ends of the second tubular member 60
Because it is located inside, it is less likely to be damaged.

One of the two mark parts (the third mark part 66a) of the second tubular member 60 is in plane contact with one of the two mark parts (the second mark part 46b) of the first tubular member 40. The other of the two mark parts of the second tubular member 60 (the fourth mark part 66b) has a shape that can be contacted with the other of the two mark parts of the second tubular member 40.
It may have a shape that can be brought into contact with the other of the two mark parts (the first mark part 46a) with a surface. An example of the shape of such a mark portion is shown in FIG. FIG. 9 shows the first tubular member 4 along line CC in FIG.
0 and the cross section of the second tubular member 60, that is, the first tubular member 40 and the second tubular member 6
The first mark portion 46a of the first tubular member 40 and the second tubular member 6 perpendicular to the stretching direction of
An example of a cross section of the fourth mark portion 66b of 0 is shown. In the example shown in FIG.
6a and the fourth mark portion 66b each have contact surfaces 41 and 61 that can come into contact with each other.
Similarly, the second mark part 46b of the first tubular member 40 and the third mark part 66a of the second tubular member 60 each have contact surfaces that can come into contact with each other.

In the example shown in FIG. 9, the first mark part 46a and the fourth mark part 66b that are in contact with each other have a circular cross section, but are not limited to this, and may have a cross section of any shape. . For example, as shown in FIG. 10, the first mark part 46a and the fourth mark part 66b that are in contact with each other are arranged in a plane that includes the longitudinal axis of the first tubular member 40 and the longitudinal axis of the second tubular member 60. It is also possible to form a plane parallel to . In this case, by bringing this surface into contact with a plate-shaped member such as a holder, the first mark part 46a and the fourth mark part 66b can be easily held while being brought into contact with each other. Similarly, the second mark part 46b and the third mark part 66a which are in contact with each other may have a cross section of any shape.

Further, one of the two mark parts (the third mark part 66a) of the second tubular member 60 is fitted into one of the two mark parts (the second mark part 46b) of the first tubular member 40. It has a shape that can be combined with the second
The other of the two mark parts (fourth mark part 66b) of the tubular member 60 of is shaped so that it can fit into the other of the two mark parts (first mark part 46a) of the first tubular member 40. It may have. Examples of shapes of such mark portions are shown in FIGS. 11 to 13. FIG. 11 shows a cross section of the first tubular member 40 and the second tubular member 60 taken along line CC in FIG. An example of a cross section of the first mark part 46a of the first tubular member 40 and the fourth mark part 66b of the second tubular member 60 is shown. FIG. 12 shows two mark parts 46a, 46b of the first tubular member 40 and two mark parts 66a, 66 of the second tubular member 60.
It is a schematic sectional view including the longitudinal axis of the 1st tubular member 40, and the longitudinal axis of the 2nd tubular member 60, showing an example of b. In FIG. 12, the first mark part 46a and the fourth mark part 66b fit into each other, and the second mark part 46b and the third mark part 66a fit into each other. FIG. 13 shows an example of a cross section of the first mark part 46a and the fourth mark part 66b taken along the line EE in FIG. 12. In the example shown in FIGS. 11 to 13, the first mark portion 46a and the fourth mark portion 66b each have uneven surfaces 43 and 63 that fit into each other. Similarly, the second mark part 46b and the third mark part 66a each have uneven surfaces that fit into each other.

The first mark part 46a and the fourth mark part 66b, and the second mark part 4 that fit into each other
6b and the third mark portion 66a may have cross sections of arbitrary shapes. For example, as shown in FIG. 13, the first mark part 46a and the fourth mark part 66b fitted into each other are arranged in a plane including the longitudinal axis of the first tubular member 40 and the longitudinal axis of the second tubular member 60. It is also possible to form a plane parallel to . In this case, by bringing this surface into contact with a plate-shaped member such as a holder, it is possible to easily hold the first mark part 46a and the fourth mark part 66b while fitting them together.

The first mark part 46a and the third mark part 66a may have the same shape, and the second mark part 46b and the fourth mark part 66b may have the same shape. In this case, the shape formed by the first mark part 46a and the fourth mark part 66b that abut or fit with each other, and the shape formed by the second mark part 46b and the third mark part 66a that abut or fit with each other. The shape formed by is the same. Thereby, between the first mark part 46a and the second mark part 46b, that is, the third mark part 66
Since the interval δ (see FIG. 12) between the first tubular member 40 and the second tubular member 60 is constant between the first tubular member 40 and the fourth mark portion 66b, The member 60 can be reliably joined. Further, the first mark part 46a and the third mark part 66a are different from the second mark part 46b.
and may have a smaller outer shape than the fourth mark portion 66b. In this case, the first tubular member 4
The liquid feeding tube obtained by joining 0 and the second tubular member 60 as described later has a first mark part 46a and a third mark part 66a with a small outer shape, but a second mark part 46a with a large outer shape. It does not have the mark part 46b and the fourth mark part 66b. Since such a liquid sending pipe has small irregularities, it has good workability.

In addition, corresponding colors (for example, the same color) and/or corresponding characters (for example, the same characters) are attached to the first mark part 46a and the fourth mark part 66b, and the second mark part 46b and the third mark part Mark part 6
A color and/or a letter corresponding to 6a may be added.

The first mark part 46a and the fourth mark part 66b, the second mark part 46b and the third mark part 66a
The first tubular member 40 and the second tubular member 40 can be made into corresponding shapes (for example, shapes that contact each other with their surfaces or shapes that fit together), or are provided with corresponding colors and/or corresponding letters. The operator who joins the tubular member 60 must ensure that the first mark part 46a and the fourth mark part 66
b can be superimposed and the second mark part 46b and the third mark part 66a can be joined by superimposing them (that is, if the first mark part 46a and the third mark part 66a are mistakenly overlapped, It is possible to prevent the second mark part 46b and the fourth mark part 66b from being joined by overlapping each other), thereby preventing the first tubular member 40 and the second tubular member 60 from being joined in the wrong direction. can. Furthermore, it is also possible to prevent the first tubular member 40 and/or the second tubular member 60 from being erroneously joined to another member.

Note that the second tubular member 60 may be provided with an additional mark part in addition to the two mark parts. That is, the second tubular member 60 includes at least two mark portions. The further mark part may have the same shape, size, color, etc. as the two mark parts, or may have a different shape, size, color, etc.

(3) Sterile conjugation kit Next, a sterile conjugation kit according to an embodiment will be explained. The aseptic bonding kit according to the embodiment includes the liquid container set according to the embodiment described above, and a sterile bonding device including a holder that holds the first tubular member and the second tubular member of the liquid container set. have The sterile joining device connects the first tubular member and the second tubular member such that the two marking portions of the first tubular member and the two marking portions of the second tubular member are both located at opposite ends of the holder. A tubular member can be held. That is, the width of the holder of the sterile joining device is the same as the distance between the two markings on the first tubular member and the distance between the two markings on the second tubular member.

Any sterile bonding device can be used as the sterile bonding device. For example, TSCD
-II (manufactured by Terumo), SCDIIB (manufactured by Terumo), HOTNAVI (manufactured by JMS), Bio
Welder (manufactured by Sartorius) or the like can be used.

At least one of the marking portions of the first tubular member and at least one of the marking portions of the second tubular member may include a holder fitting portion that can fit into a holder of the sterile bonding device.

(4) Aseptic joining method A sterile joining method using the liquid storage container, liquid storage container set, and sterile joining kit according to the embodiment will be described with reference to FIGS. 14 and 15.

As shown in FIG. 14, the first tubular member 40 and the second tubular member 60 are held in a holder 80 of a sterile bonding device. At this time, the two mark portions 46a and 46b of the first tubular member 40
The first tubular member 40 is positioned with respect to the holder 80 such that the first tubular member 40 is located at each end of the holder 80. Similarly, the second tubular member 60 is positioned with respect to the holder 80 so that the two mark portions 66a and 66b of the second tubular member 60 are located at both ends of the holder 80, respectively.

The sterile bonding device is then operated according to the instructions for using the sterile bonding device. The sterile joining device cuts the first tubular member 40 and the second tubular member 60 while maintaining the flow path 42 of the first tubular member 40 and the flow path 62 of the second tubular member 60 in a closed space. and the first tubular member 4
0 and the cut portions of the second tubular member 60 are joined together. As a result, as shown in FIG.
The tubular member 40 and the second tubular member 60 are joined via the joint 58, and the first tubular member 4
0 and the second tubular member 60 are formed. The liquid feeding pipe 50 has a liquid feeding path 52 formed by communicating the flow path 42 of the first tubular member 40 and the flow path 62 of the second tubular member 60 therein. This enables liquid feeding via the liquid feeding path 52. Liquid feeding may be performed using a liquid feeding mechanism such as a peristaltic pump, a syringe pump, a diaphragm pump, or a pressurizing pump, or may be performed using gravity.

The first and second tubular members 40, 60 are provided with marking portions 46a, 46b, 66a, 66b that indicate the installation position of the holder 80 of the sterile bonding device. Therefore, the operator who performs aseptic joining can visually and/or tactually recognize which position of the first and second tubular members 40 and 60 should be held by the holder 80. Therefore, the liquid sending pipe 50 formed
The length of is constant regardless of the worker.

As shown in FIG. 14, when the outer surfaces 45, 65 of the first and second tubular members 40, 60 are convex at the marking portions 46a, 46b, 66a, 66b, the holder 80 of the aseptic joining device is , the two mark portions 46a and 46b of the first tubular member 40 and the second tubular member 60
Since the holder 80 is sandwiched between the two mark portions 66a and 66b of the first tubular member 4,
The holder 80 and the first and second tubular members 40 and 60 are prevented from being displaced relative to each other by sliding in the extending direction of the holder 80 and the second tubular member 60 .

Marking portions 46a, 46b, 66 perpendicular to the extending direction of the first and second tubular members 40, 60
When the cross-sectional shape of a and 66b is anisotropic (i.e., when the cross-sectional shape is other than a circle,
For example, when the cross-sectional shape is triangular as in the example shown in FIG.
, 66b (the rotation angle of the mark parts 46a, 46b around the longitudinal axis of the first tubular member 40, and the rotation angle of the mark parts 66a, 66b around the longitudinal axis of the second tubular member 60)
Accordingly, each twist of the first and second tubular members 40 and 60 can be recognized visually and tactilely. Therefore, it is possible to prevent the first and second tubular members 40 and 60 from being joined in a twisted state.

In FIG. 14, the mark parts 46a, 46b, 66a, and 66b each have holder fitting parts 49a, 49b, 69a, and 69b that can be fitted into the holder 80. Thereby, it is determined more accurately where on the first tubular member 40 and the second tubular member 60 the holder 80 of the sterile bonding device is installed. Also, the mark portions 46a, 46b, 66a, 6 for the holder 80
6b (the rotation angle of the mark parts 46a, 46b around the longitudinal axis of the first tubular member 40, and the rotation angle of the mark parts 66a, 66b around the longitudinal axis of the second tubular member 60) is Since it is constant, it is possible to prevent the first tubular member 40 and the second tubular member 60 from being joined in a twisted state. Note that the holder fitting parts 49a, 49b, 69a, and 69b are not essential components. That is, the holder fitting portion may be provided in one of the two marking portions 46a, 46b of the first tubular member 40 and one of the two marking portions 66a, 66b of the second tubular member 60. However, neither of the first and second tubular members 40 and 60 may be provided with a holder fitting portion.

In order to operate the sterile bonding device to form the liquid delivery tube 50, the first tubular member 40 and the second tubular member 60 are placed in the holder 80 in a predetermined orientation and position determined by the sterile bonding device. It is necessary to install it. For example, in the example shown in FIG. 14, the right side is the first
It is necessary to position the first tubular member 40, which is closed by the closing part 48, above the second tubular member 60, which is closed on the left side by the second closing part 68a. If the sterile joining device is operated with the first and second tubular members 40, 60 upside down, the first tubular member 40, 60
After the second tubular member 60 is cut, the portion of the first tubular member 40 including the first closing portion 48 and the portion of the second tubular member 60 including the second closing portion 68a are joined. Therefore, the portion of the first tubular member 40 that does not include the first closing portion 48 and the portion of the second tubular member that does not include the second closing portion 68a are not joined to each other. As a result, the liquid feeding tube 50 cannot be obtained. Therefore, the first tubular member 40 and the second tubular member 60 need to be installed in the correct orientation and position.

Here, as in the examples shown in FIGS. 9 to 13, the first mark part 46a and the second mark part 46b of the first tubular member 40 are the fourth marks of the second tubular member 60, respectively. part 66b and third
When the first mark part 46a and the second mark part 46b of the first tubular member 40 have a shape corresponding to the mark part 66a (for example, a shape that can be brought into contact with a surface or a shape that can be fitted), , the first tubular member 40 and the second tubular member 60 are held in a holder by making them come into contact with or fitting into the fourth mark part 66b and the third mark part 66a of the second tubular member 60, respectively. Set it at 80.
Therefore, it is possible to prevent the first tubular member 40 and the second tubular member 60 from being installed in the holder 80 in a state different from the predetermined orientation and position. Further, since the rotation angles of the first tubular member 40 and the second tubular member 60 about the longitudinal axes can be made constant, the first and second tubular members 40 and 60 are connected in a twisted state. It can also be prevented from happening.

(5) Application of liquid storage container The liquid storage container according to the embodiment is usually used to store a liquid that requires aseptic handling. For example, a culture container for culturing cells, a medium supply container for supplying a medium, a cell collection container for collecting a cell suspension after culture, a waste liquid container for collecting a medium during or after culture, or a temporary cell container. The liquid storage container according to the embodiment can be used as a general cell-related container such as a storage container, or in a cell culture system. In particular, it can be suitably used in cell culture systems. Examples of other liquids that require aseptic handling include food-related liquids, pharmaceutical-related liquids, and the like. Examples of food-related liquids include beverages, seasonings, and the like. Pharmaceutical liquids include electrolyte infusions such as physiological saline, carbohydrate injections such as glucose, blood products, protein drugs such as antibiotics and antibodies, low molecular weight proteins, peptide drugs such as hormones, nucleic acid drugs, Cell medicines, vaccines to prevent various infectious diseases, steroids, insulin, anticancer drugs, protease inhibitors, analgesics, antipyretic, analgesic and antiinflammatory agents, anesthetics, fat emulsions, antihypertensive agents, vasodilators, heparin Examples include electrolyte correction injections such as sodium chloride and potassium lactate, vitamin preparations, and contrast agents. However, the uses of the liquid storage container are not limited to these.

EXAMPLES Hereinafter, the present invention will be specifically explained with reference to Examples, but the present invention is not limited to these Examples.

Two tubes with an inner diameter of 3 mm and an outer diameter of 4 mm were prepared as the first and second tubular members.
Two landmarks were formed by wrapping and fixing two cable ties around each tube. In both tubes, the distance between the landmarks was made equal to the width of the holder of the sterile joining device. Each tube was placed in a sterile splicer holder such that the two cable ties were located at each end of the sterile splicer holder. Since the position of each tube with respect to the holder is fixed by the cable ties, even if the tube is pulled in the direction of tube stretching,
The tube did not move relative to the holder. When the sterile bonding device was activated, 2
The tubes were sterilely spliced.

Although the embodiments of the present invention have been described in detail above, the present invention is not limited to the above embodiments, and various design changes can be made without departing from the spirit of the present invention as described in the claims. It can be performed.

1 Liquid storage container 20 Container body 22 Storage space 24 Opening 40 First tubular member 41, 61 Contact surface 42, 62 Channel 43, 63 Uneven surface 44 Inner surface 45, 65 Outer surface 46a, 46b, 66a, 66b Mark Part 47 Opening 48 First closing portion 49a, 49b, 69a, 69b Holder fitting portion 50 Liquid feeding tube 52 Liquid feeding path 58 Joint portion 60 Second tubular member 68a Second closing portion 68b Third closing portion 80 holder

Claims (1)

A liquid storage container,
a container body defining a liquid accommodation space;
a first tubular member connected at one end to the container body;
a first closing portion that closes the first tubular member;
Equipped with
The first tubular member has an inner surface that defines, together with the first closure, a flow path communicating with the accommodation space;
a first landmark portion, wherein the first tubular member is disposed at different positions between the one end of the first tubular member and the first closing portion in the extending direction of the first tubular member; and a second mark portion.

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