JP2023118164A - Skin topical composition - Google Patents

Abstract

To provide an external composition for skin that is made from a natural product and has excellent biological safety, and produces the effects of preventing skin aging, ameliorating a change of skin color and rendering the skin healthy.SOLUTION: The present invention provides a proteasome activity enhancer that contains extract from adzuki beans, belonging to the Fabaceae family, as an active ingredient, and an external composition for skin containing the agent.SELECTED DRAWING: Figure 1

Description

TECHNICAL FIELD The present invention relates to a functional material for external skin composition (including cosmetics and quasi-drugs) combining niacinamide and adzuki bean extract.

The skin is affected by internal factors such as inactivation of cell proliferation and differentiation, decreased hormone secretion, and quantitative decrease in extracellular matrix components, as well as active oxygen and air pollutants induced by sunlight (ultraviolet rays). , chemical substances such as environmental hormones, allergens such as pollen, and external factors such as environmental stress are intricately intertwined to cause aging phenomena, rough skin, and changes in color tone.

In addition, recent studies have shown that intracellular proteasomes are also involved in senescence of human cells. The proteasome is a giant enzyme complex that is distributed both in the cytoplasm and the nucleus in eukaryotic cells and performs intracellular protein degradation. For example, proteasome decomposes proteins (cross-linked, glycated or carbonylated proteins) degraded by ultraviolet rays and active oxygen, prevents the accumulation of these degraded proteins in cells, and maintains and improves the functions of cells such as skin. known to contribute to

In order to prevent skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed, and cosmetics containing these active ingredients (skin-beautifying ingredients, whitening ingredients, etc.) have been put on the market. It is For example, antioxidants such as vitamin C, vitamin E and catalase; anti-inflammatory agents such as glycyrrhizic acid; various UV absorbers; agents; extracellular matrix components such as collagen, elastin, and hyaluronic acid; and humectants such as urea. Also, arbutin, kojic acid and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, freckles and chloasma, and are widely used as active ingredients in whitening agents.

As described above, conventionally, functional materials having a skin-healthy effect have been proposed based on various mechanisms of skin aging, but there are problems in terms of safety and efficacy for the skin. . Therefore, there is a demand for a functional material improved in this respect.

The present inventors conducted intensive research in view of the problems of the prior art, and found that the combination of niacinamide and the adzuki bean extract, which is a leguminous plant, exhibits a pronounced synergistic effect of enhancing proteasome activity. It has been found that, as a result, it is possible to provide an external composition for skin that exhibits an anti-aging effect and a healthy skin effect and is excellent in skin safety.

Conventionally, the use of adzuki beans or saponins derived from adzuki beans as moisturizing agents and skin barrier function improving agents has been publicly known by the descriptions in Patent Documents 1 to 5, etc. However, it is possible to combine niacinamide and azuki bean extracts. It was not known that the synergistic effect of enhancing proteasome activity was exhibited by

JP-A-2001-131047 Japanese Patent Application Laid-Open No. 2001-288066 JP-A-2002-265324 JP-A-2003-137768 Japanese Patent Application Laid-Open No. 2016-074609

The present invention is a proteasome activity enhancer containing niacinamide and adzuki bean extract as active ingredients.
The present invention also provides an external composition for skin containing the above agent.

The present invention is a proteasome activity enhancer containing niacinamide and an adzuki bean extract as active ingredients, and according to the present invention, a composition for external use on the skin that exhibits remarkably excellent anti-aging effects and skin-healthy effects. can also be provided.

FIG. 2 is a diagram showing the proteasome activity enhancement effect of the present invention.

Preferred embodiments of the present invention are described in detail below.
The present invention is a proteasome activity enhancing agent containing niacinamide and an extract of adzuki bean (Vigna angularis or Phaseolus angularis), which is a plant of the Fabaceae family. Niacinamide is a kind of water-soluble B vitamins and is also called nicotinamide. The adzuki bean used in the present invention is not limited to a specific variety, and any variety may be used. Moreover, in the present invention, as the part to be extracted from azuki bean, seeds, the whole plant containing seeds, or a combination of seeds and other parts (flowers, stems, roots, leaves, etc.) can be used.

For the preparation of the extract, first, parts of azuki beans (for example, seeds, whole plants containing seeds, or a combination of seeds and other parts (flowers, stems, roots, leaves, etc.)) are washed with water in advance if necessary. After removing the foreign matter by squeezing, it is dried as it is or, if necessary, it is cut into small pieces or pulverized, and extracted by bringing it into contact with an extraction solvent. The extraction method can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but it is also possible to use a supercritical extraction method or a steam distillation method.

Examples of extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; Used.

Among these extraction solvents, water and lower alcohols are used in the present invention from the viewpoint of the effectiveness of the extract obtained, further from the viewpoint of skin irritation, and from the viewpoint of wide application to cosmetics. Hydrophilic solvents such as polyhydric or polyhydric alcohols are preferred. Preferable examples when using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols (especially ethanol), or the use of a mixed solvent of water and polyhydric alcohols (especially 1,3-butylene glycol, glycerin), etc. Among them, water alone, or water and 1,3 - Mixed solvents of butylene glycol are particularly preferred.

When a mixed solvent is used, the mixing ratio is, for example, a mixed solvent of water and 1,3-butylene glycol is 1:1 to 20:1 in terms of volume ratio (hereinafter the same), and a mixed solvent of water and ethanol is used. If so, it is preferably in the range of 1:1 to 25:1, and in the case of a mixed solvent of water and glycerin, it is preferably in the range of 1:1 to 20:1.

In addition, the weight ratio of the dried parts of adzuki beans (e.g., seeds, the whole plant containing seeds, or a combination of seeds and other parts (flowers, stems, roots, leaves, etc.)) to the extraction solvent is preferably 1:1. to 1:50, more preferably 1:5 to 1:20.

When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 4-9. In this sense, if necessary, the extraction solvent is blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid. may be adjusted to a pH of

Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. , the extraction temperature is preferably in the range of 0° C. to 80° C., more preferably 0° C. to 20° C., and the extraction time is preferably 1 hour to 1 week, more preferably 4 hours. ~3 days.

Prior to the extraction process of the present invention, or in parallel with the extraction process, adzuki bean may be hydrolyzed as needed. As a result, there is a possibility that the storage stability of the adzuki bean extract can be improved, and the extract can be used more effectively as a cosmetic compounding agent.

The extract prepared as described above may generally be adjusted to a pH of 4 to 8 and then used as it is as a cosmetic compounding agent, or may be used at a desired concentration by concentration under reduced pressure or the like. You can Also, the extract may be dried by a conventional method such as a spray drying method.

In order to improve storage stability and the like, the extract prepared as described above may be stored in a refrigerator for a certain period of time, and the supernatant may be used as a cosmetic compounding agent.

Examples of external skin compositions (cosmetics, quasi-drugs, etc.) containing the adzuki bean extract of the present invention include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, and blushes. , white powder, facial cleanser, body shampoo, hair shampoo, soap, etc., and also hair growth agents and bath additives, but are not limited to these.

The amount of the adzuki bean extract of the present invention in the external composition for skin is generally 0.0001 to 0.75% by weight (solid content by weight, the same shall apply hereinafter) in the case of basic cosmetics, as the solid content of the extract. It is preferably in the range of 0.0005 to 0.5% by weight, and in the case of make-up cosmetics, it is generally in the range of 0.0001 to 0.5% by weight, preferably 0.0002 to 0.5% by weight. For cosmetics, the range is generally 0.0001 to 0.75% by weight, preferably 0.0001 to 0.5% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.0001 to 0.5% by weight, preferably 0.0001 to 0.3% by weight.

Further, the content of niacinamide in the composition for external use on skin is preferably 0.01% by weight to 10% by weight.

In cosmetics, in addition to the adzuki bean extract as an essential ingredient, ingredients that are usually used in cosmetics, such as oily ingredients, surfactants (synthetic, natural products), moisturizers, thickeners, preservatives and sterilizers. Agents, UV protection agents, powder components, antioxidants, pigments, perfumes, etc. can be appropriately blended as needed. In addition, as long as the effectiveness and characteristics of the adzuki bean extract are not impaired, it may be blended with other physiologically active ingredients in combination.

Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, Plant-derived oils and fats such as macadamia nut oil and plant-derived squalane; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax and lanolin; liquid paraffin, petrolatum, paraffin wax, squalane and the like Hydrocarbons of; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Synthetic esters such as isopropyl, butyl oleate, 2-ethylhexylglyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) and synthetic triglycerides are included.

Examples of surfactants include polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, poly Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkylphenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, tri cationic surfactants such as alkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylenepolyamine fatty acid amide salts; N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N',N'-dimethyl-N'- Amphoteric surfactants such as β-hydroxypropylammoniosulfobetaine and the like can be used.

Examples of emulsifiers or emulsifying aids include enzyme-treated stevia and other stevia derivatives, saponin or its derivatives, casein or its salts (sodium, etc.), complexes of sugar and protein, sucrose or its esters, lactose, and soybean-derived water-soluble emulsifiers. Polysaccharide, complex of soybean-derived protein and polysaccharide, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives , lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, grains, etc.), etc. can also be added.

Moisturizing agents include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, and mucopolysaccharides (eg, hyaluronic acid). acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, natural and synthetic sphingosaccharide lipids (including water-soluble and hydrophobic), intercellular lipids and their relatives Substances (eg, ceramide 1-3, ceramide 5, ceramide 6-2, ceramide AG, ceramide AP, ceramide AS, ceramide EOP, ceramide NG, ceramide NP, ceramide NS), NMF-related substances, lactic acid, urea, higher fatty acid octyl Dodecyl, seaweed extracts, serrata root (white and white) extracts, various amino acids and derivatives thereof.

Examples of thickening agents include alginic acid, agar, carrageenan, fucoidan, and other brown algae, green algae, or red algae-derived components; Polysaccharides; gums such as xanthan gum, tragacanth gum, guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, acrylic acid/methacrylic acid copolymer; and derivatives thereof; polyglutamic acid and derivatives thereof;

Examples of antiseptic/bactericidal agents include urea; paraoxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; Ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, dry distillation of bark, fermented radish, plant-derived ethanol such as sugarcane, or 1, 3-butylene glycol and the like.

Examples of UV protection agents include titanium oxide, zinc oxide, cerium oxide, ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2, 4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, Examples include ethyl urocanate and aloe extract.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose. cereals (rice, wheat, corn, millet, etc.) powders, beans (soybeans, adzuki beans, etc.) powders, etc.

Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof (eg, vitamin E nicotinate, vitamin E linoleate, etc.).

Whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone and its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, - methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate) , adenosine monophosphate), and these may be blended singly or in combination.

Examples of the above-mentioned kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate and kojic acid dibutyrate; kojic acid ethers; kojic acid sugar derivatives such as kojic acid glucoside; However, ascorbic acid derivatives include, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 - Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbate, caprylyl Ascorbic acid sugar derivatives such as 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L- L-ascorbic acid tetrafatty acid esters such as ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylation derivatives, ascorbic acid glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. However, hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives include tranexamic acid esters (eg, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or salts thereof), amides of tranexamic acid (e.g., tranexamic acid methylamide), etc., and resorcinol derivatives such as 4-n-butylresorcinol and 4-isoamylresorcinol. 2,5-dihydroxybenzoic acid derivatives such as 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives Examples include nicotinamide and benzyl nicotinate, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like.

Anti-wrinkle agents include vitamin A or derivatives thereof, vitamin E or derivatives thereof (tocopherol acetate, etc.), pantothenyl alcohol, tranexamic acid, allantoin, and the like.

Examples of physiologically active ingredients include placenta extract, saxifrage extract, saxifrage extract, perilla extract, rice bran extract or its hydrolyzate, white mustard extract or its hydrolyzate, fermented white mustard, peony extract or hydrolyzate thereof, lactic acid bacteria fermented rice, purple extract, lotus seed extract or hydrolyzate thereof, fermented lotus seed, extract or hydrolyzate thereof, hydrolyzate of adlay, fermented adlay seed, Royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. be done. In addition, coral grass extract, rice leaf extract or its hydrolyzate, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, apricot fruit extract, katamen eucheuma Seaweed extracts such as seaweed extracts, marine flowering plant extracts such as eelgrass, fermented soymilk, jellyfish water, rice extract or its hydrolyzate, fermented rice extract, germinated rice extract or its hydrolyzate, germinated rice Fermented products, black soybean extracts or hydrolysates thereof, damask rose flower extracts, bamboo shoot skin extracts, linoleic acid and its derivatives or processed products (e.g., liposomal linoleic acid), animal- or fish-derived collagen and derivatives thereof, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract licorice extract, carrot extract, red ginseng extract, honey kelp extract, luffa extract, sea lettuce extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract , pau d'arco bark extract, daylily extract or ferment, hibiscus flower extract or ferment, red wormwood extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red Fuki extract, Shiran extract, Pepper pericarp or seed coat extract or hydrolyzate, Safflower flower extract, Casablanca extract, Sweet potato extract or fermented product, Guava leaf extract, Houttuynia cordata extract, Banpeiyu extract , aloe extract, strawberry flower extract, apple extract, etc.

Next, the present invention will be described in more detail with production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all percentages mean weight %.

Production example 1. Preparation of adzuki bean seed extract 1200 g of a mixed solvent of purified water and 1,3-butylene glycol (volume ratio 1:1) was added to 150 g of dried adzuki bean seeds. After adding the mixed solvent, extraction was performed at 4°C. The extract was filtered to obtain 820 g of a pale yellow transparent adzuki bean seed extract (solid concentration: 1.1%).

Production example 2. Preparation of extract of adzuki bean whole plant 1200 g of a mixed solvent of purified water and 1,3-butylene glycol (volume ratio 1:1) was added to 150 g of dried whole adzuki bean plant including seeds. After adding the mixed solvent, extraction was performed at 4°C. The extract was filtered to obtain 780 g of a pale yellow and transparent azuki bean whole plant extract (solid concentration: 0.8%).

Example 1. Lotion [Ingredients] Part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Phenoxyethanol 0.1
Niacinamide 1.0
Extract of Production Example 1 1.0
Ceramide 0.1
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 2. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 2.0 parts of the extract of Production Example 2 was used in place of the extract of Production Example 1 contained in the B component of Formulation Example 1.

Formulation example 3. Emulsion [Ingredients] Part Squalane 6.0
Hexalane 4.0
Jojoba oil 1.0
Decaglyceryl distearate 2.0
Soy lecithin 1.5
Niacinamide 1.0
Extract of Production Example 1 1.0
L-ascorbic acid-2-glucoside 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Dipotassium glycyrrhizinate 0.1
Phenoxyethanol 0.1
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 4. Milky Lotion A milky lotion was obtained in the same manner as in Formulation Example 3, except that 2.0 parts of tranexamic acid was used in place of 2.0 parts of L-ascorbic acid-2-glucoside in component B of Formulation Example 3.

Formulation example 5. Milky Lotion A milky lotion was obtained in the same manner as in Formulation Example 3, except that 3.0 parts of arbutin was used in place of 2.0 parts of L-ascorbic acid-2-glucoside in component B of Formulation Example 3.

Formulation example 6. Milky lotion A milky lotion was obtained in the same manner as in Formulation Example 3, except that 3.0 parts of 3-O-ethylascorbic acid was used in place of 2.0 parts of L-ascorbic acid-2-glucoside in component B of Formulation Example 3. .

Formulation example 7. Milky Lotion A milky lotion was obtained in the same manner as in Formulation Example 3, except that 5.0 parts of chamomile extract was used in place of 2.0 parts of L-ascorbic acid-2-glucoside in component B of Formulation Example 3.

Formulation example 8. Milky lotion A milky lotion was obtained in the same manner as in Formulation Example 3, except that 5.0 parts of Sohakuhi extract was used in place of 2.0 parts of L-ascorbic acid-2-glucoside in component B of Formulation Example 3.

Formulation example 9. Emulsion [Ingredients] Part Liquid paraffin 6.0
Hexalane 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Niacinamide 1.0
Extract of Production Example 1 2.0
Glycerin 3.0
Ceramide 0.1
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Phenoxyethanol 0.1
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 10. Lotion [Ingredients] Part Niacinamide 1.0
Extract of Production Example 1 10.0
Dipotassium glycyrrhizinate 0.1
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Pantothenyl alcohol 0.1
Phenoxyethanol 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 11. Lotion A lotion was obtained in the same manner as in Formulation Example 12, except that 10.0 parts of the extract of Production Example 2 was used in place of the extract of Production Example 1 in the ingredients of Formulation Example 10.

Formulation example 12. Essence [Ingredient] Part Niacinamide 1.0
Extract of Production Example 1 2.0
Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Phenoxyethanol 0.1
Hyaluronic acid 0.1
Citric acid 0.3
Sodium citrate 0.6
Purified water Amount that makes the total amount 100 parts

Formulation example 13. Essence An essence was obtained in the same manner as in Formulation Example 12, except that the extract 2.0 of Production Example 2 was used in place of the extract of Production Example 1 in the ingredients of Formulation Example 12.

Example 14. Liquid foundation [Ingredients] Part Polyoxyethylene sorbitan tristearate 2.4
Cetostearyl alcohol 0.2
Liquid Lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Niacinamide 1.0
Extract of Production Example 1 3.0
Carboxymethylcellulose sodium 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Phenoxyethanol 0.1
Titanium oxide 8.0
Talc 4.0
Color pigment Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 15. Body Shampoo [Ingredients] Part N-lauroylmethylalanine sodium 25.0
Coconut fatty acid potassium liquid (40%) 26.0
Coconut fatty acid diethanolamide 3.0
Phenoxyethanol 0.1
Extract of Production Example 2 2.0
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Formulation example 16. Hair restorer [Ingredients] Part dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-menthol 0.8
Tamasakitsutsurafuji root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Niacinamide 1.0
Extract of Production Example 1 3.0
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
Ethanol 20.0
Appropriate amount of L-arginine Purified water Amount that makes the total amount 100 parts

Formulation example 17. Hair Shampoo [Ingredient] Part Sodium N-coconut oil fatty acid methyl taurate 10.0
Sodium polyoxyethylene (3) alkyl ether sulfate 20.0
Lauryldimethylaminoacetate betaine 10.0
Coconut fatty acid diethanolamide 4.0
Phenoxyethanol 0.1
Citric acid 0.1
Niacinamide 1.0
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Example 18. Hair conditioner [Ingredients] Part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Phenoxyethanol 0.1
Niacinamide 1.0
Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Purified water Amount that makes the total amount 100 parts

Test example 1. Effect of enhancing proteasome activity Human adult-derived fibroblasts NHDF were seeded at 1×10 ⁴ cells/well in Eagle's minimal essential medium containing 0.5% NCS at 1×10 4 cells/well under conditions of 37° C., 5.0% CO _{2 .} After pre-cultivating for 1 day, (1) niacinamide (NA) alone is added as a sample solution to the culture medium of adult human fibroblasts NHDF so that the concentration is 1 mM; The extract is added as a sample solution to a concentration of 1.0% and 2.0% (as a solution), respectively. Furthermore, the extract of Production Example 1 was added as a sample solution to a concentration of 1.0% and 2.0% (as a solution), respectively. It was cultured for an additional 3 days. After that, remove the cell culture supernatant of each test group and add 100 μL of cell lysate (Tris-HCL buffer (pH 8.0) containing 1% Tween 20: 12 mM trishydroxymethylaminomethane, 5 mM EDTA, 150 mM NaCl, hydrochloric acid). A crude enzyme solution was obtained by crushing the cells using 50 μL of the crude enzyme solution was placed on a separate plate, and 10 μL of proteasome activity inhibitor (cell lysate containing 2 mM epigallocatechin gallate) was added thereto for non-proteasome activity measurement. In addition, 10 μL of cell lysate was added to the remaining crude enzyme solution to adjust the volume, and this was used for total substrate resolution measurement. 10 μL of a buffer containing 65 μM Suc-Leu-Leu-Val-Tyr-AMC as a substrate was added to each plate and reacted at 37° C. for 1 hour. After completion of the reaction, fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The value obtained by subtracting the non-proteasome activity measurement result from the obtained total substrate resolution measurement result was defined as the proteasome activity. On the other hand, in the case where only PBS(-) was added instead of the above sample solution and no sample was added (control), the same procedure as above was performed for each of human neonatal fibroblasts NB1RGB and human adult fibroblasts NHDF. The relative value of the proteasome activity at the time of addition of each sample to the adult-derived fibroblast proteasome activity value obtained here was determined and defined as the proteasome activity rate (%).

The results of Test Example 1 are shown in FIG. As shown in FIG. 1, it was confirmed that adult human fibroblasts NHDF had lower proteasome activity than human neonatal fibroblasts NB1RGB. In addition, while niacinamide alone (NA in FIG. 1) did not exhibit the effect of enhancing proteasome activity, it was found that the combination of niacinamide and the extract of Production Example 1 exhibited a synergistic effect on proteasome activity. shown. As shown in FIG. 1, when the concentration of the extract of Production Example 1 is 0.5%, by using it together with niacinamide, the value of the extract (0.5%) of Production Example 1 and niacinamide alone A synergistic effect about 10% higher than the effect of adding is confirmed, and when the concentration of the extract of Production Example 1 is 1.0%, when used in combination with niacinamide, the extraction of Production Example 1 Approximately 27% higher synergistic effect was observed compared to the additive effect of each value of mono (1.0%) niacinamide. Thus, by blending niacinamide and the extract according to the present invention in combination, it is possible to provide an external composition for skin that exerts an anti-aging effect and a skin-healthy effect.

Claims (2)

A proteasome activity enhancer containing niacinamide and adzuki bean extract as active ingredients. An external skin composition comprising the agent according to claim 1 .