JP2022058157A - Nucleic acid detection host, and nucleic acid detection device - Google Patents

Abstract

To provide a nucleic acid detection device whose whole structure is simple, and which has a simple detection operation, low expertise requirement to a work process, high detection efficiency, and a drastically reduced detection cost.SOLUTION: A nucleic acid detection host 10 comprises a housing 11, a detection kit mounting region 12, a heating region 13, a sample filling region 14 and an image sampling device, in which the detection kit mounting region is provided in the housing, and in which a detection kit is mounted, the heating region is provided in the housing, houses detection liquid and heats the detection liquid, the sample filling region is provided in the housing, positioned in the detection kit mounting region, communicates with the detection kit mounting region, inputs the detection liquid into the detection kit in the detection kit mounting region, the image sampling device is provided on one side remote from the sample filling region in the detection kit mounting region, and samples an image of the detection kit in the detection kit mounting region.SELECTED DRAWING: Figure 1

Description

The present application relates to nucleic acid detection hosts and nucleic acid detection devices.

Detection for current molecular diagnostics, morphology, immunology, etc. is often done in a fixed laboratory, is time consuming, inefficient, inflexible, and can be detected anytime, anywhere. There is a problem that it cannot be done, and especially patients who carry a strongly infectious virus are easily infected with others on the way to the fixed detection point, and there is a concern about safety.

In view of this, it is necessary to provide a nucleic acid detection host in order to overcome at least one of the above defects.
The present invention also provides a nucleic acid detection device.

The present invention provides a nucleic acid detection host comprising a housing, a detection kit mounting region provided in the housing, a heating region, a sample filling region, and an image acquisition device. The detection kit mounting area is for mounting the detection kit. The heating region is for accommodating the detection liquid and heating the detection liquid. The sample filling area is provided in the detection kit mounting area and communicates with the detection kit mounting area, and the sample filling puts the detection liquid in the detection kit in the detection kit mounting area. The image collecting device is provided on the side far from the detection kit mounting area, and is for collecting images of the detection kit in the detection kit mounting area.

In the embodiment of the present application, the detection kit mounting area is provided on the mounting tank, the through hole provided in the wall near the sample filling region in the mounting tank, and the side far from the sample filling region in the mounting tank. The image taking port, the fixing box provided on the side far from the sample filling area in the image taking port, the detection kit engaging port provided on the side far from the sample filling area in the mounting tank, and the detection kit. It comprises a fixing mechanism provided on the side of the engagement port far from the sample filling area, the fixing mechanism for engaging with the detection kit through the detection kit engagement port, and the sample filling area. The image acquisition device is provided in the fixed box and collects the image through the image taking port.

In embodiments of the present application, the end of the mounting tank closer to the sample filling region is higher than the far end.

In an embodiment of the present application, the fixing mechanism comprises a solenoid valve and a top block provided on the solenoid valve closer to the mounting tank, the top block being located at the detection kit engagement port and the solenoid valve. Allows the top block to enter the inside of the mounting tank through the detection kit engagement port so as to fix or open the detection kit.

In an embodiment of the present application, the heating region includes a heating tank and a heating device provided at the bottom of the heating tank.

In the embodiment of the present application, a first sensor provided in the detection kit mounting area and sensitive to the detection cassette and a second sensor provided in the heating region and sensitive to the detection liquid are further provided.

In an embodiment of the present application, the nucleic acid detection host further comprises a display for displaying the image and a camera for collecting identification information corresponding to the detection solution.

The present invention also provides a nucleic acid detection device comprising a host, a collection cup, a sample filling device, and a detection kit. The host is a nucleic acid detection host as described above. The collection cup is detachably provided in the heating region and is for collecting and heating the detection liquid. The sample filling device is detachably provided in the collection cup or the sample filling area, and the detection liquid is sucked in a fixed amount from the collection cup and detected by the detection kit through the sample filling area. Add the liquid. The detection kit is detachably provided in the sample filling region, and is for performing a PCR amplification reaction and electrophoresis detection of the detection solution.

In embodiments of the present application, the detection kit comprises a box, a sample filling port, a detection chip, an electrophoresis cassette, a detection window and a connector. The sample filling port is provided on the side of the box body close to the sample filling region. The detection chip is provided inside the box and communicates with the sample filling area through the sample filling port. The electrophoresis cassette is provided inside the box and communicates with the detection chip. The detection window is provided on the side of the box body close to the image acquisition device and corresponds to the electrophoresis cassette. The connector is provided inside the box and is electrically connected to the detection chip and the electrophoresis cassette, respectively.

More than before, the nucleic acid detection device provided by the present invention can perform amplification and electrophoresis detection of nucleic acid in one device by the cooperation of the host and the detection kit, and the overall structure is simple. Yes, the detection operation is simple, the work process requires less professional requirements, the detection efficiency is high, and the detection cost is extremely reduced. In addition, the detection process is highly flexible, and the detection equipment is portable without having to do it in a fixed laboratory, so community detection and home detection can be realized.

It is a structural schematic diagram of the nucleic acid detection host which concerns on one Embodiment of this invention. It is a schematic diagram of the internal structure of the nucleic acid detection host which concerns on one Embodiment of this invention. It is a schematic cross-sectional structure diagram of the nucleic acid detection host which concerns on one Embodiment of this invention. It is a structural schematic diagram of the fixing mechanism which concerns on one Embodiment of this invention. It is a structural schematic diagram of the detection kit and the image acquisition apparatus which concerns on one Embodiment of this invention. It is a structural schematic diagram of the nucleic acid detection device which concerns on one Embodiment of this invention. It is a structural schematic diagram of the detection kit which concerns on one Embodiment of this invention. It is an exploded view of the detection kit which concerns on one Embodiment of this invention. It is a figure which shows the structure of the sample filling apparatus which concerns on one Embodiment of this invention. It is a structural schematic diagram of the collect cup which concerns on one Embodiment of this invention. It is a structural schematic diagram of the drug bag which concerns on one Embodiment of this invention. It is a schematic diagram which shows the detection process of the nucleic acid detection device which concerns on one Embodiment of this invention. It is a schematic diagram which shows the detection process of the nucleic acid detection device which concerns on one Embodiment of this invention. It is a schematic diagram which shows the detection process of the nucleic acid detection device which concerns on one Embodiment of this invention. It is a schematic diagram which shows the detection process of the nucleic acid detection device which concerns on one Embodiment of this invention. It is a schematic diagram which shows the detection process of the nucleic acid detection device which concerns on one Embodiment of this invention. It is a flowchart of the nucleic acid detection method which concerns on one Embodiment of this invention.

Hereinafter, the technical proposal in the examples of the present invention will be described clearly and thoroughly with reference to the drawings in the examples of the present invention, but of course, the described examples are a partial embodiment of the present invention. It is just an example, not all examples.

It is important to note that when an assembly is said to be "fixed to" another assembly, it may be directly on or within the other assembly. .. If one assembly is considered to be "connected" to another assembly, it may be directly attached to the other assembly, or it may be an assembly that coexists in it. If one assembly is considered to be "provided in" another assembly, it may be provided directly on the other assembly, or it may be an assembly coexisting therein. The terms "vertical," "horizontal," "left," and "right" and similar descriptions used in this document are for illustration purposes only.

The embodiments in the system described below are schematic, and the above-mentioned module and circuit divisions are merely logical functional divisions, and may be other divisions in actual realization. Furthermore, it can be seen that the word "contains" does not exclude other units or steps, and the singular does not exclude the plural. The plurality of units or devices described in the claims of a system may also be implemented by one unit or device through software or hardware. Words such as first and second are used to represent names and do not represent a particular order.

Unless otherwise specified, all terms used herein have the same meaning as the usual meanings understood by engineers in the art of the invention. The terms used herein are for purposes of illustration only, and are not intended to limit the invention. As used herein, the term "and / or" includes any combination of one or more related enumerated elements.

In addition, referring to FIGS. 1 and 2, the embodiment of the present invention provides a nucleic acid detection host 10, and the nucleic acid detection host 10 is a housing 11 and a detection kit mounting area 12 provided in the housing 11. The heating region 13, the sample filling region 14, the image acquisition device 15, and the controller 16 are included, and the controller 16 is electrically connected to the heating region 13 and the image acquisition device 15.

The heating region 13 collects a nucleic acid sample of a subject and mixes the nucleic acid sample with a detection agent (for example, a buffer solution) to form a detection solution. The heating region 13 also heats the detection liquid under the control of the controller 16. The sample filling region 14 is located above the detection kit mounting region 12 and communicates with the detection kit mounting region 12. The detection kit mounting area 12 is for mounting the detection kit 20, and the detection kit 20 and the controller 16 are electrically connected to each other. The sample filling region 14 is for injecting the detection liquid into the detection kit in the detection kit mounting region 12 and performing a PCR amplification reaction and electrophoresis detection on the detection liquid in the detection kit 20. The image collecting device 15 is provided on the side far from the sample filling area 14 in the detection kit mounting area 12, and is for collecting an image of the detection kit 20 in the detection kit mounting area 12 under the control of the controller 16. It is a thing. The image is a fluorescence photograph of electrophoresis detection, and the detection result can be obtained from this fluorescence photograph.

With reference to FIG. 1, the housing 11 connects the first surface 111, the second surface 112 provided facing the first surface 111, and the first surface 111 and the second surface 112. A side wall 113 and a second side wall 114 provided so as to face the first side wall 113 are provided. The opening of the detection kit mounting area 12 is located on the first side wall 113, and the detection kit 20 is left in the detection kit mounting area 12 through the opening of the first side wall 113. The openings of the sample filling region 14 and the heating region 13 are both located on the first surface 111.

With reference to FIG. 3 and a combination of FIGS. 1 and 2, the detection kit mounting region 12 includes a mounting tank 121 and an image taking port 122 provided on the side of the mounting tank 121 far from the sample filling region 14. The fixing box 123 provided on one side of the image taking port 122 close to the second surface 112, the detection kit engaging port 124 provided on the surface of the mounting tank 121 far from the sample filling region 14, and the detection kit clerk. It includes a fixing mechanism 125 provided on one side of the abutment 124 close to the second surface 112, and a first inductor 126 provided on the housing 11 and electrically connected to the controller 16. The detection kit 20 is inserted into the mounting tank 121, and the fixing mechanism 125 engages the detection kit 20 with the bottom of the detection kit 20 through the detection kit engagement port 124 so that the detection kit 20 can be attached to and detached from the mounting tank 121. To fix. The sample filling region 14 communicates with the mounting tank 121 through a through hole (not shown), and further adds a sample to the detection kit 20. The image taking device 15 is provided in the fixed box 123, and can take a fluorescent photograph of the detection kit 20 through the image taking port 122. The first inductor 126 is for detecting whether or not the detection kit 20 has been inserted into the mounting tank 121 and transmitting a signal to the controller 16, which controls the detection kit 20 to detect nucleic acids. ..

With reference to FIG. 3 and in combination with reference to FIG. 2, the mounting tank 121 is an inclined designed groove, specifically, the end near the sample filling region 14 is higher than the far end. When many bubbles are generated in the PCR reaction process and the generated bubbles stay in the detection flow path, the operation path of the liquid ball in the detection flow path is obstructed by the bubble, the liquid ball becomes immovable, and the detection fails. do. Therefore, by designing the mounting tank 121 at an angle, the detection kit 20 can be placed at an angle, the sample filling end of the detection kit 20 is higher than the end where the PCR amplification reaction occurs, and the PCR in the detection kit 20 is performed. Bubbles in which the reaction occurs can naturally move to the upper side, are naturally discharged from the sample filling end of the detection kit 20, and do not interfere with the operation path of the liquid ball.

In the present embodiment, the shape of the mounting tank 121 is designed according to the shape of the detection kit 20, and specifically, the mounting tank 121 is a substantially rectangular groove.

With reference to FIG. 4 and a combination of FIGS. 2 and 7, the fixing mechanism 125 comprises one solenoid valve 1251 and a top block 1252 provided on the solenoid valve 1251 on the bottom surface of the detection kit 20. An engagement groove 25 that engages with the top block 1252 is provided, the top block 1252 is located at the detection kit engagement port 124, and the solenoid valve 1251 is electrically connected to the controller 16. When the detection kit 20 is inserted into the mounting tank 121, the first inductor 126 responds to the detection kit 20 and transmits a detection signal to the controller 16, which controls and energizes the solenoid valve 1251. Further, the top block 1252 is pushed up and inserted into the engaging groove 25 to fix the detection kit 20. When the test is completed, the controller 16 controls and energizes the solenoid valve 1251, further flips up the top block 1252, and pops up the detection kit 20 from the mounting tank 121. By providing the solenoid valve 1251 and the first inductor 126, the detection kit 20 can be automatically locked and automatically popped up. It can be seen that the automatic engagement and pop-up of the detection kit 20 may be achieved by other engagements.

With reference to FIG. 3, the first inductor 126 is for detecting whether or not the detection kit 20 is inserted or popped up in the mounting tank 121, while the controller 16 is automatically sensitive to the sensitivity of the first inductor 126. A boot program may be implemented. That is, when it is detected that the detection kit 20 has been inserted into the mounting tank 121, the program for starting the detection is started. When the pop-up of the detection kit 20 is detected, the program for ending the detection is started.

With reference to FIG. 3, the image taking port 122 has a substantially rectangular structure, so that the image taking device 15 can collect a complete fluorescent photograph obtained by the electrophoretic detection of the detection kit 20 from the image taking port 122 in the size setting. Conditions are met.

With reference to FIGS. 3 and 5, with reference to FIG. 1 in combination, the cross-sectional width of the fixed box 123 gradually widens from the end near the first surface 111 to the end far from it, i.e. It has a funnel structure with a substantially inverted conical shape. The end of the fixed box 123 near the first surface 111 communicates with the mounting tank 121 via the image taking port 122, and the image collecting device 15 is provided at one end of the fixed box 123 away from the first surface 111. The image collecting device 15 is located directly below the image taking port 122.

With reference to FIGS. 1 and 3, in this embodiment, the opening of the mounting tank 121 is located on the first side wall 113 and is provided with a front baffle 115 to close or open the opening. The front baffle 115 is slidably provided in the opening of the mounting tank 121, and specifically, the front baffle 115 moves along the first side wall 113 toward the second surface 112 to open the opening. The opening may be closed by moving toward the first surface 111 along the first side wall 113.

With reference to FIGS. 2 and 3, the heating region 13 includes a heating tank 131 and a heating device 132 provided at the bottom of the heating tank 131, the heating device 132 being electrically connected to the controller 16. The controller 16 detects the heating temperature and time of the heating tank 131 by the temperature inductor (not shown) and the time relay (not shown) while controlling the heating device 132 to realize the temperature rise. Can be done. In the present embodiment, the heating temperature of the heating device 132 is about 95 ° C., the heating time is about 5 minutes, and after the heating is completed, the cooling is performed at room temperature or a specific temperature (for example, 40 ° C. or lower). Cool to.

In this embodiment, the heating device 132 is composed of an aluminum block, a copper block or other heat conductive material. The heating tank 131 may be heated by another heating device (for example, a heating thread, a heating coating, a heating sheet, or the like). At the same time, a second inductor (not shown) is provided in the heating region 13, the second inductor is electrically connected to the controller 16, and a container for accommodating the detection liquid is placed in the heating tank 131. The controller 16 is used to activate the heating by sensing whether or not the capacitor is in use and transmitting a sensitive signal to the controller 16.

In the present embodiment, the opening of the heating tank 131 is located on the first surface 111, and the cross section along the direction parallel to the first surface 111 has a substantially elliptical structure. The shape of the heating tank 131 can be specifically designed according to the shape of the container according to the actual heating requirement.

Referring to FIG. 3, the sample filling region 14 includes a sample filling tank 141 and a sample filling passage 142, the opening of the sample filling tank 141 is located on the first surface 111, and the sample filling passage 142 is used for sample filling. One end of the sample filling passage 142, which penetrates the region 14 and enters the mounting tank 121 and is separated from the sample filling tank 141, abuts on the surface of the detection kit 20 of the mounting tank 121, and the detection liquid is discharged from the sample filling tank 141. It enters the detection kit 20 of the mounting tank 121 via the sample filling passage 142.
In this embodiment, the sample filling tank 141 is specifically funnel-shaped.

With reference to FIG. 5 and in combination with reference to FIG. 2, the image acquisition device 15 includes a light source (not shown) electrically connected to the controller 16 and an image acquisition device 151. This light source is for emitting light toward the image taking port 122 under the control of the controller 16, and provides a light source for image acquisition. The image collector 151 collects fluorescence photographs obtained by electrophoresis detection of the detection kit 20 under the control of the controller 16.

In the present embodiment, the image acquisition device 15 is housed in the fixed box 123, and the side wall of the fixed box 123 is provided so as to be inclined so as to achieve the purpose of condensing, and the light emitted from the light source is taken as an image taking port. The light is focused on 122 to facilitate the collection of images of the detection kit 20. A reflective coat layer is provided on the inner side wall of the fixed box 123, and the light rays can be reflected and the light rays can be reflected and entered into the image taking port 122.

With reference to FIGS. 2, 3 and 5, the controller 16 includes a master plate 161, a feeding plate 162, a detection kit control plate 163, and an image acquisition control plate 164. Therefore, the power supply plate 162 is electrically connected to the master plate 161, the detection kit control plate 163, and the image acquisition control plate 164 to supply power to the entire device. The detection kit control plate 163 is electrically connected to the detection kit 20 and controls the nucleic acid detection process of the detection kit 20. The image acquisition control plate 164 is electrically connected to the light source and the image acquirer 151, controls the light emitting source of the light source, and controls the acquisition of the detected image of the detection kit 20 by the image acquirer 151. The heating device 132, the first inductor 126, and the second inductor are all electrically connected to the master plate 161.

In the present embodiment, the image acquisition control plate 164 includes an image processor (not shown). The image collected by the image collector 151 is transferred to an image processor for processing, and the processed image is further output.

In the present embodiment, the controller 16 further includes a memory (not shown) for storing the detection result, the detection process information, and the like.

With reference to FIGS. 1 and 2, the nucleic acid detection host 10 further includes a display 17 and a camera 18, both of which are electrically connected to the master plate 161 of the controller 16. The display 17 is for displaying an operation interface, setting operation parameters, displaying the image, and the like. The camera 18 can capture the flow of user operations and also collect related information (for example, information on the origin of the nucleic acid sample) corresponding to the detection liquid.

With reference to FIG. 2, the nucleic acid detection host 10 further includes a heat dissipation device 19 that is electrically connected to the master plate 161 of the controller 16 and dissipates heat to the entire host.

In the present embodiment, the heat dissipation device 19 is a heat dissipation fan. The heat radiating device 19 is provided on the second side wall 114. At the same time, the housing 11 is provided with a plurality of heat dissipation vents so that heat inside the host can be discharged.

INDUSTRIAL APPLICABILITY The present invention indicates that the nucleic acid detection host 10 cooperates with the detection kit 20 to realize PCR amplification and electrophoresis detection of nucleic acid, and the result displayed on the display 17 is the final electrophoresis detection result. The nucleic acid detection host 10 to be used is provided. The nucleic acid detection host 10 integrates the heating of the detection liquid, sample filling, detection, and output of the result in one device, and the overall structure is simple, portable, highly flexible in detection, and convenient in detection operation. Moreover, the operation process is simple and the professional requirements are low, enabling home detection.

With reference to FIG. 6, the present invention further provides a nucleic acid detection device 100 including a nucleic acid detection host 10, a detection kit 20, a collection cup 30, and a sample filling device 40 as described above. The detection kit 20 is detachably provided in the detection kit mounting area 12, the collection cup 30 is detachably provided in the heating region 13, and the sample filling device 40 is detachably provided in the sample filling region 14. A sample filling device 40 is detachably fixed to the collection cup 30. The collection cup 30 is for placing the detection liquid, and is also put in the heating region 13, so that the heating device 132 in the heating region 13 heats the detection liquid. The sample filling device 40 quantitatively sucks the detection liquid from the collection cup 30, and puts the detection liquid into the detection kit 20 from the sample filling region 14. This detection kit 20 is for performing a PCR amplification reaction and electrophoresis detection on a detection solution.

With reference to FIGS. 7 and 8 and a combination of FIGS. 2 and 5, the detection kit 20 in the present application integrates the PCR amplification process and the electrophoresis detection, and the detection solution is directly electrophoresed after the PCR amplification is completed. Enter the tank and perform electrophoresis detection. Specifically, the detection kit 20 includes a box body 21, a sample filling port 22, a detection chip 23, an electrophoresis cassette 24, a detection window 26, and a connector 27. The sample filling port 22 is provided on the side close to the sample filling area 14 in the box body 21, and one end of the sample filling passage 142 away from the sample filling tank 141 abuts on the sample filling port 22 to fill the sample. The mouth 22 is for putting the detection liquid in the detection chip 23. The detection chip 23 and the electrophoresis cassette are provided inside the box 21 and communicate with the detection chip 23 and the electrophoresis cassette 24. The detection chip 23 is for performing a PCR amplification reaction of the detection liquid, and the electrophoresis cassette 24 is used. The purpose is to perform electrophoresis detection of the detection liquid after the completion of amplification. The detection window 26 is provided on the side close to the image taking port 122 in the box body 21 and corresponds to the electrophoresis cassette 24, and the image acquisition device 15 passes through the image taking port 122 and the detection window 26 to the electrophoresis cassette 24. Take a fluorescent photo of the window. The engagement groove 25 that engages with the fixing mechanism 125 of the detection kit mounting area 12 is provided on the image acquisition device 15 side of the box body 21. The connector 27 is provided on the side of the box 21 close to the detection kit control plate 163, is electrically connected to the detection kit control plate 163, and is electrically connected to the detection chip 23 and the electrophoresis cassette 24. The electrophoresis cassette 24 is installed in the same box 21 together with the detection chip 23, and after the nucleic acid amplification reaction is completed, electrophoresis detection becomes possible automatically, the process becomes smooth, and device replacement is not required. The sample filling control becomes accurate. The detection kit 20 integrates the detection chip 23 and the electrophoresis cassette 24 into one cassette, and because of its small size, it is applied to the portable nucleic acid detection device 100 described above.

In this embodiment, the detection kit 20 is a disposable item, and one detection kit 20 is used for each detection sample, so that the detection kit 20 does not require a cleaning flow.

In this embodiment, the detection kit 20 has a substantially cubic structure.

With reference to FIG. 10 and in combination with reference to FIG. 6, the collection cup 30 and the sample filling device 40 are used in cooperation and are connected by engaging the two, and the collection cup 30 is a nucleic acid sample. (For example, a drool or other liquid sample) is collected and mixed with a detection reagent to form a detection solution, which is used to heat the heating region 13. The sample filling device 40 quantitatively sucks the detection liquid from the collection cup 30 and puts the detection liquid into the detection kit 20 via the sample filling region 14.

In the present embodiment, a tapered groove is provided inside the collecting cup 30, and after the drool is spit out in the collecting cup 30, it is collected under the tapered groove to facilitate the collection of a small amount of nucleic acid sample. Become.

With reference to FIG. 9, the sample filling device 40 includes a lower case 41, an upper case 42, a liquid collection assembly 43 and a push button 44. Therefore, the upper case 42 is movably connected to the lower case 41 by engagement, the liquid collection assembly 43 is provided so as to penetrate the lower case 41 and the upper case 42, and the push button 44 is the top of the upper case 42. It is provided in. The liquid collection assembly 43 includes an elastic bag structure. When liquid collection is required, the upper case 42 moves downward along the lower case 41 by pressing the push button 44, and the upper case 42 applies pressure to the liquid collection assembly 43 to deform the liquid collection assembly 43. After the air inside the elastic capsule is discharged, the detection liquid is sucked, and the liquid collection is completed, the liquid collection assembly 43 can push the upper case 42 to automatically return to the original position. When it is necessary to discharge the liquid, press the push button 44 again to move the upper case 42 downward with respect to the lower case 41, and further press the liquid collection assembly 43 to discharge the detection liquid in the elastic bag. Let me. By controlling the amount of deformation of the elastic bag, the amount of liquid collected can be controlled, and the purpose of quantitative liquid collection can be achieved. The sample filling device 40 has a simple overall structure, low cost, easy operation, and can achieve the purpose of quantitative liquid collection. It can also be understood that liquid transfer is possible with a sample filling device that can separately realize quantitative liquid collection.

With reference to FIG. 11, the nucleic acid detection device 100 further includes a drug bag 50 in which the detection drug (eg, buffer) is stored, and the detection drug is quantitatively placed in the drug bag 50. The drug bag 50 can be placed in a collection cup 30 and mixed with a nucleic acid sample to form the detection solution.

In the present embodiment, the drug bag 50 has a tank-shaped structure that houses a drug necessary for nucleic acid detection and has a handle, and the opening is sealed with a sealing membrane. At the time of use, the sealing membrane is torn, the handle is grasped, the drug is laid down in the collection cup 30 containing the nucleic acid sample, shaken, and then the collection cup 30 is placed in the heating tank 131 and heated.

Prior to detection, the detection kit 20, the sample filling device 40, the collection cup 30, and the drug bag 50 are all housed in a dedicated package, and the detection kit 20, the sample filling device 40, the collection cup 30, and the collection cup 30 are housed in a dedicated package. An identification code (for example, QR code) corresponding to one-to-one is provided for each drug bag 50 to avoid confusion. Since the detection for the same virus is the same for the detection kit 20 and the drug bag 50 used, an identification code (for example, a QR code) is provided only on the collection cup 30 to avoid confusion of the collected liquid to be detected. You can also do it.

Specifically, the camera 18 collects the QR code on the collection cup 30, and the information of the QR code may include the origin of the nucleic acid sample or the information of the subject.

With reference to FIG. 17, the present invention includes a method for detecting nucleic acid using the nucleic acid detection device 100, specifically, the following steps.

In step S11, the parameters are set with reference to FIG.

The nucleic acid detection host 10 can also be turned on to set the corresponding detection parameters, specifically the heating temperature of the heating region 13, the heating time, the parameters of the PCR amplification process in the detection kit 20, and the electrophoresis detection. Each parameter of can be included.

In step S12, with reference to FIG. 12, the detection sample information is input and recording is started.

Specifically, the camera 18 is opened to collect the QR code on the collection cup 30, the detection process is recorded, and the package containing the detection kit 20, the collection cup 30, and the drug bag 50 is opened and collected. Scan the QR code on the collection cup 30. The collected information and recorded materials will be uploaded to the client for confirmation by the parties concerned.

In step S13, the detection kit 20 is inserted into the detection kit mounting area 12 with reference to FIG.

When the detection kit 20 is inserted into the mounting tank 121 of the detection kit mounting area 12 and the insertion of the detection kit 20 is detected, the detection kit 20 is automatically locked and the preheating process in the detection flow is automatically started.

In step S14, referring to FIG. 14, a nucleic acid sample is collected to form a detection solution, and the detection solution is heated.

In the present embodiment, the drool is collected by the collection cup 30, and the drug in the drug bag 50 is further tilted into the collection cup 30, and the drug bag 50 is hung in the opening of the collection cup 30 in the reverse direction. It is sufficient to cover 30 and swing it up and down 3 to 5 times, and generally swing it 5 times to obtain a uniform detection liquid. Next, the collection cup 30 on which the detection liquid is placed is placed in the heating region 13, and when the second inductor in the heating region 13 detects the charging of the collection cup 30, the next step is started and heating is performed. The heating temperature set by a general program is about 90 to 100 ° C., about 3 to 8 minutes, and after heating, it is cooled to room temperature or a certain temperature or less (for example, 40 ° C. or less). Specifically, the heating temperature and the heating time are sensed by using a temperature inductor and a time relay.

In another embodiment, after the drool is collected by the collection cup 30, it is first placed in the heating region 13 and heated, and the heating temperature set by a general program is about 90 to 100 ° C., 3 to 8 min. It is heated to a certain degree, and after heating, it is cooled to room temperature or a constant temperature or lower (for example, 40 ° C. or lower), and after cooling, the drug in the drug bag 50 is put into the drool of the collection cup 30 and the detection liquid is uniformly mixed.

In step S15, with reference to FIGS. 15 and 16, the detection solution is transferred to the detection kit 20 for PCR amplification reaction and electrophoresis detection.

The detection liquid 10 to 30 μL (preferably 20 μL) in the collection cup 30 is quantitatively sucked by the sample filling device 40, and the detection liquid is added to the detection chip 23 of the detection kit 20 via the sample filling region 14. Be done. Specifically, the detection solution containing the nucleic acid sample undergoes a PCR amplification reaction in the detection chip 23, and after the amplification is completed, it is combined with the fluorescent reagent in the detection chip 23 to form a product having a fluorescent group, and the product is produced. The object enters the electrophoresis cassette 24 from the detection chip 23, and the electrophoresis is detected in the electrophoresis cassette 24.

In step S16, an image (fluorescent photograph) of electrophoresis detection is collected and output.

After the electrophoresis detection is completed, the image acquisition device 15 acquires an image of electrophoresis through the detection window 26 of the electrophoresis cassette 24, processes the image with an image processor, and displays the processed image on the display 17. Alternatively, the detection result may be uploaded to the client and viewed by the parties concerned.

In step S17, the detection is terminated.

The collection cup 30, the sample filling device 40, and the detection kit 20 are removed from the nucleic acid detection host 10 and placed in a package for recovery.

More than before, the nucleic acid detection device provided by the present invention can perform amplification and electrophoresis detection of nucleic acid in one device by the cooperation of the host and the detection kit, and the overall structure is simple. Yes, the detection operation is simple, the work process requires less professional requirements, the detection efficiency is high, and the detection cost is extremely reduced. In addition, the detection process is highly flexible, and the detection equipment is portable without having to do it in a fixed laboratory, so community detection and home detection can be realized.

100 Nucleic acid detection display 10 Nucleic acid detection host 11 Housing 111 First side 112 Second side 113 First side wall 114 Second side wall 115 Front baffle 12 Detection kit mounting area 121 Mounting tank 122 Image pickup port 123 Fixed box 124 Detection kit engagement port 125 Fixing mechanism 1251 Solenoid valve 1252 Top block 126 First inductor 13 Heating area 131 Heating tank 132 Heating device 14 Heating area 141 Heating tank 142 Heating device 15 Image collection device 151 Image collection device 16 Controller 161 Master plate 162 Power supply plate 163 Detection kit control Plate 164 Image collection control plate 17 Display 18 Camera 19 Heat dissipation device 20 Detection kit 21 Box body 22 Sample filling port 23 Detection chip 24 Nucleic acid cassette 25 Engagement groove 26 Detection window 27 Connector 30 Collection cup 40 Sample filling device 41 Lower case 42 Upper case 43 Liquid collection assembly 44 Push button 50 Drug pack

Claims (10)

Equipped with housing, detection kit mounting area, heating area, sample filling area, image acquisition device,
The detection kit mounting area is provided in the housing, and the detection kit is mounted.
The heating region is provided in the housing, contains the detection liquid, and heats the detection liquid.
The sample filling area is provided in the housing, is located in the detection kit mounting area, communicates with the detection kit mounting area, and puts the detection liquid in the detection kit in the detection kit mounting area.
The image acquisition device is provided on one side of the detection kit mounting region away from the sample filling region, and is characterized in that an image of the detection kit in the detection kit mounting region is collected. The detection kit mounting area includes a mounting tank, a through hole provided on one side wall of the mounting tank near the sample filling region, and an image taking port provided on the side of the mounting tank far from the sample filling region. The fixing box provided on the side far from the sample filling area in the image taking port, the detection kit engaging port provided on the side far from the sample filling area in the mounting tank, and the sample filling in the detection kit engaging port. Equipped with a fixing mechanism provided on the side far from the area,
The fixing mechanism is for fixing to the detection kit through the detection kit engagement port.
Claim 1 is characterized in that the sample filling region communicates with a mounting tank through the through hole, and the image collecting device is provided in the fixing box and collects the image through the image taking port. Nucleic acid detection host according to. The nucleic acid detection host according to claim 1, wherein one end closer to the sample filling region in the mounting tank is higher than one end farther from the sample filling region in the mounting tank. The fixing mechanism includes a solenoid valve and a top block provided on the solenoid valve near the mounting tank.
The top block is located at the detection kit engagement port.
2. The solenoid valve according to claim 2, wherein the solenoid valve drives the top block to enter the inside of the mounting tank through the detection kit engagement port so as to fix or open the detection kit. Nucleic acid detection host. The nucleic acid detection host according to claim 1, wherein the heating region includes a heating tank and a heating device provided at the bottom of the heating tank. The first aspect of the present invention is characterized in that a first sensor provided in the detection kit mounting area and sensitive to the detection cassette and a second sensor provided in the heating region and sensitive to the detection liquid are further provided. The nucleic acid detection host described. The nucleic acid detection host according to claim 1, further comprising a display for displaying the image and a camera for collecting identification information corresponding to the detection liquid. A host that is the nucleic acid detection host according to any one of claims 1 to 7.
A collection cup that is detachably provided in the heating area to collect and heat the detection liquid,
A sample filling device that is detachably provided in the collection cup or the sample filling region, sucks the detection liquid in a fixed amount from the collection cup, and puts the detection liquid in the detection kit through the sample filling region. When,
A nucleic acid detection device comprising a detection kit detachably provided in the sample filling region and detachably provided in a detection kit mounting region for performing PCR amplification reaction and electrophoresis detection of the detection liquid. .. The detection kit is
With the box
A sample filling port provided on the side of the box body close to the sample filling region,
A detection chip provided inside the box and communicating with the sample filling region via the sample filling port,
An electrophoresis cassette provided inside the box and communicating with the detection chip,
A detection window provided in the box body close to the image acquisition device and corresponding to the electrophoresis cassette, and provided in the box body and electrically connected to the detection chip and the electrophoresis cassette, respectively. The nucleic acid detection device according to claim 8, further comprising a connector. Further equipped with a drug bag to store the detected drug,
The nucleic acid detection device according to claim 8, wherein the detection agent and the nucleic acid sample form the detection liquid.

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