JP2019010025A - Tyrosinase activity inhibitor and food and drink containing the same - Google Patents

Abstract

An object of the present invention is to provide a tyrosinase activity inhibitor that has an excellent tyrosinase activity inhibitory action and can be widely used in the field of food and cosmetics. An excellent tyrosinase activity inhibitor is prepared by blending an extract of a chamomileaceae chamomile flower part and an extract of a rose family peach genus bud part at a mass ratio of 25:75 to 75:25. Can be provided. [Selection figure] None

Description

  The present invention relates to a tyrosinase activity inhibitor. More specifically, the present invention relates to a tyrosinase activity inhibitor characterized by being able to significantly suppress the activity of tyrosinase that causes melanin production.

  In recent years, interest in anti-aging has increased greatly with the extension of the average life. Antioxidant components, which are the key to human anti-aging, are applied in the form of cosmetics and supplements, but there is an increasing demand for plant extracts that are applied to the human body in a more natural form. For example, in Patent Document 1, as a whitening component, screening is performed on a wide variety of plant extracts, and the whitening effect superior to the dragon fruit extract is derived. For plant extracts eaten or consumed as supplements, higher physiological activity is required.

The plant extract has a flavor peculiar to plants, and when it is used as a supplement, a peculiar flavor is imparted to provide a pleasant naturalness. However, since human taste is sensitive and is repeatedly consumed as food and drink, if the physiological activity of the plant extract is not sufficient, an excessive flavor is imparted to the effect, which is a natural comfort. It deviates from goodness and arouses a sense of “getting bored”. This is a problem unique to supplements not found in cosmetics applied to the skin.
In particular, although plant extracts of flower parts and buttocks have been known to function as antioxidants (for example, Patent Document 2), plant extracts sufficient for use in the form of supplements as a whitening ingredient are still not available. A plant extract that has not been found and has a high whitening performance while having a good flavor is desired.

JP 2013-166713 A JP 2007-161591 A

  An object of the present invention is to provide a tyrosinase activity inhibitor that has an excellent tyrosinase activity inhibitory action and can be widely used in the field of foods and cosmetics.

As a result of intensive studies on the above problems, the present inventors have found that the above problems can be solved by using a chamomile flower part extract and a peach cocoon part extract at a specific ratio. As a result, the present invention has been completed.
That is, this invention is the following [1]-[2].

[1] A tyrosinase activity inhibitor comprising an extract of a chamomileae chamomile flower part and an extract of a rose family peach genus bud part at a mass ratio of 25:75 to 75:25.
[2] A food or drink comprising the tyrosinase activity inhibitor according to [1].

  ADVANTAGE OF THE INVENTION According to this invention, the tyrosinase activity inhibitor which can be widely used in the field | area of a foodstuff or cosmetics can be provided.

The present invention relates to an "extract of asteraceae chamomile chamomile flower part (hereinafter abbreviated as" camomile extract ")" and "extract of rosaceae peach genus cocoon part (hereinafter" peach extract "). ) "In a mass ratio of 25:75 to 75:25 (hereinafter sometimes referred to as" tyrosinase activity inhibitor of the present invention "). .)
The “camomile” of the “extract of chamomile flower part” used in the present invention is an Asterales (Matricaria) chamomile (Chamomilla recutita L.), "Peach" is a Rosarys peach genus (Amygdalus) peach (Prunus persica Batsch).
Further, the “extract” means a liquid, solid, or a mixture obtained by a known extraction method used for extracting plant components, and the extraction solvent used for extraction is removed, It shall mean the state which does not contain an extraction solvent substantially.
Extraction methods include immersion extraction method, continuous extraction method, steam distillation extraction method (normal pressure, reduced pressure), supercritical fluid extraction method, squeeze extraction method, etc. Among them, extraction solvents such as immersion extraction method are used. The extraction method is particularly preferred.
Hereinafter, an extraction method using an extraction solvent such as an immersion extraction method will be described in detail with specific examples.

As a specific procedure of the immersion extraction method, the chamomile flower part and the peach cocoon part are extracted by immersing them in an extraction solvent, and the chamomile flower part and the peach cocoon part are extracted by a known solid-liquid separation method such as filtration. Examples of the method include separating the liquid and concentrating by distilling off the extraction solvent, or purifying and concentrating by a process such as column chromatography or solvent fractionation.
It is preferable that the chamomile flower part and the peach cocoon part used for extraction are dried in order to improve extraction efficiency. Examples of the drying method include sun drying, air drying, heat drying, vacuum drying, and airflow drying.
Moreover, it is preferable that the chamomile flower part and the peach cocoon part used for extraction are coarsely crushed in order to improve extraction efficiency. Examples of the crushing method include using a crusher such as a uniaxial crusher or a biaxial crusher.

  The type of the extraction solvent is not particularly limited, and a known solvent used for plant extraction can be appropriately selected. Specific examples thereof include aliphatic hydrocarbons such as hexane, cyclohexane, and heptane; benzene, toluene, and the like. Aromatic hydrocarbons; Esters such as ethyl acetate and butyl acetate; Ketones such as methyl ethyl ketone and acetone; Ethers such as diethyl ether and methyl-t-butyl ether; Halogenated hydrocarbons such as dichloromethane and trichloroethylene; , Propylene glycol, 1,3-butylene glycol and other alcohols; water and the like. The solvent may be a mixed solvent in which two or more types are combined. When the extraction solvent contains alcohols, the extraction efficiency of the active ingredient is increased, and when the extraction solvent contains a bactericidal solvent such as ethanol, an extract that is less susceptible to spoilage can be obtained. Furthermore, in the production process, there are advantages such as a short concentration time of the extract and excellent washability of the container after extraction.

When the extraction solvent is a mixed solvent containing water, the content of water in the extraction solvent is usually 1% by mass or more, preferably 5% by mass or more, more preferably 10% by mass or more, and further preferably 30% by mass or more. In general, it is 99% by mass or less, preferably 95% by mass or less, more preferably 90% by mass or less, and further preferably 70% by mass or less.
When the extraction solvent is a mixed solvent containing alcohols, the content of alcohol in the extraction solvent is usually 1% by mass or more, preferably 5% by mass or more, more preferably 10% by mass or more, and further preferably 30% by mass. These are usually 99% by mass or less, preferably 95% by mass or less, more preferably 90% by mass or less, and still more preferably 70% by mass or less.
When the extraction solvent is a mixed solvent of water and alcohol, the mass ratio of water and alcohol (water / alcohol) is usually 0.01 or more, preferably 0.1 or more, more preferably 0.4 or more. Usually, it is 100 or less, preferably 10 or less, more preferably 2.0 or less.
Within the above range, it becomes easy to obtain an extract excellent in tyrosinase activity inhibitory action.

  When obtaining a chamomile extract, it is preferable to use a mixed solvent of water and alcohols, and it is particularly preferable to use a mixed solvent of water and ethanol. The mass ratio of water to alcohol (water / alcohols) is preferably 0.1 or more and 10 or less. The lower limit is preferably 0.4 or more, more preferably 0.7 or more, and particularly preferably 0.9 or more. The upper limit is preferably 2.5 or less, more preferably 1.4 or less, and particularly preferably 1.1 or less.

When obtaining a peach extract, it is preferable to use water or a mixed solvent of water and alcohol, and it is particularly preferable to use water.
When a mixed solvent of water and alcohol is used, it is more preferable to use a mixed solvent of water and ethanol. The mass ratio of water to alcohol (water / alcohols) is preferably 2 or more, more preferably 10 or more, still more preferably 15 or more, and particularly preferably 30 or more.

  The amount of the extraction solvent used in the extraction is usually at least 1 time, preferably at least 2 times, more preferably at least 5 times, and even more preferably at least 10 times in terms of mass relative to the chamomile flower or peach cocoon. Yes, usually 10,000 times or less. Within the above range, it becomes easy to obtain an extract excellent in tyrosinase activity inhibitory action.

The extraction temperature should be appropriately selected according to the type of the extraction solvent, etc., but is usually greater than 0 ° C., preferably 5 ° C. or higher, more preferably 20 ° C. or higher, more preferably 50 ° C. or higher, Usually, it is 200 ° C. or lower, preferably 150 ° C. or lower, more preferably 100 ° C. or lower.
The extraction time should be appropriately selected according to the extraction temperature and the like, but is usually 1 minute or longer, preferably 10 minutes or longer, more preferably 30 minutes or longer, more preferably 1 hour or longer, usually 1 week or shorter. It is.

The chamomile extract and peach extract extracted by the above extraction operation may be used as the state of the extract dispersed in the extraction solvent, or the solid content (high boiling point) from which the extraction solvent has been removed by evaporation to dryness. It may also be used as oil). Moreover, when removing an extraction solvent, it is preferable to set it as the powder composition impregnated with inert powders, such as dextrin, starch, and lactose. The powder composition can be obtained by pouring a predetermined amount of inert powder into the extract and then evaporating and drying the extraction solvent.
The input amount of the inert powder is appropriately determined according to the content of the extract in the extract, and is usually 1 to 10000 parts by mass with respect to 100 parts by mass of the extract. The lower limit is preferably 5 or more, more preferably 10 or more, and particularly preferably 25 or more. As an upper limit, Preferably it is 1000 or less, More preferably, it is 400 or less, More preferably, it is 200 or less, Most preferably, it is 110 or less. In addition, content of the extract in an extract can be computed by measuring solid content.

  The tyrosinase activity inhibitor of the present invention is characterized by containing a chamomile extract and a peach extract in a mass ratio of 25:75 to 75:25. The mass ratio of chamomile extract and peach extract is preferably Is 40:60 to 75:25, more preferably 40:60 to 60:40. Within the above range, a tyrosinase activity inhibitor excellent in tyrosinase activity inhibitory action can be obtained.

  Although the tyrosinase activity inhibitor of this invention can be utilized as food / beverage products, a pharmaceutical, a quasi-drug, and cosmetics, it is preferable to utilize as food / beverage products. The tyrosinase activity inhibitor of the present invention may be for oral intake or for percutaneous intake. In addition, when the tyrosinase activity inhibitor is for transdermal intake, the dosage form may be a known one such as lotion, emulsion, gel, cream, powder, granule, capsule, sheet, spray, aerosol, paste, pack, etc. And may contain known components as appropriate according to the dosage form.

  When the tyrosinase activity inhibitor of the present invention is used as a product such as a food or drink, a pharmaceutical product, a quasi-drug, or a cosmetic, the addition amount of the tyrosinase activity inhibitor is not particularly limited, but the chamomile extract and the peach extract are combined. As a total content, it is preferable to add about 0.0001-10 mass% in a product. The lower limit is more preferably 0.01% by mass or more, still more preferably 0.05% by mass or more, and particularly preferably 0.1% by mass or more. As an upper limit, More preferably, it is 5 mass% or less, More preferably, it is 3 mass% or less, Most preferably, it is 1 mass% or less.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, this invention is not limited to these Examples.

(Preparation Example 1)
100 g of the chamomile flower part was added to 900 g of 50% by mass water-containing ethanol, heated and stirred at 60 ° C. for 3 hours, and the filtrate obtained by filtration was concentrated under reduced pressure. 1 g of dextrin was added to and mixed with 3.5 g of the solid content (the chamomile extract) in the filtrate and lyophilized. The freeze-dried product was then pulverized to obtain a chamomile extract powder composition.

(Preparation Example 2)
100 g of white peach blossom was added to 900 g of ion-exchanged water, heated and stirred at 80 ° C. for 3 hours, and the filtrate obtained by filtration was concentrated under reduced pressure. 1 g of dextrin was added to 3.3 g of the solid content (peach extract) in the filtrate, mixed and freeze-dried. Next, the freeze-dried product was pulverized to obtain a peach extract powder composition.

(Preparation Example 3)
Extracts were obtained in the same manner as in Preparation Example 2 for thyme and gerbil. However, the input amount of dextrin was the same as the solid content in the filtrate.

(Example 1: Measurement of tyrosinase activity inhibition)
First, each extract was prepared to a predetermined concentration using 0.3 M phosphate buffer (pH 6.8), and used as a sample solution.

  The enzyme solution was prepared as follows. Tyrosinase (derived from mushrooms, manufactured by Sigma) was prepared to be 0.01% by mass using a 0.3 M phosphate buffer (pH 6.8) to obtain an enzyme solution.

  The substrate solution was prepared as follows. L-DOPA (manufactured by Sigma) was prepared using a 0.3 M phosphate buffer (pH 6.8) so as to be 0.02% by mass, and used as a substrate solution.

  The test solution was prepared as follows. After adding the sample solution to the 96-well plate at 50 μl / well, the enzyme solution is added at 25 μl / well, the substrate solution is added at 25 μl / well, and the plate is set on a plate reader at 37 ° C. for 60 minutes. After incubation, the absorbance at 475 nm was measured.

  The test blank solution was prepared as follows. After adding the sample solution to the 96-well plate at 50 μl / well, add the enzyme solution at 25 μl / well, and then add 0.3 M phosphate buffer (pH 6.8) at 25 μl / well instead of the substrate solution. After incubation at 37 ° C. for 60 minutes, the absorbance at 475 nm was measured.

  The control solution was prepared as follows. After adding 0.3 M phosphate buffer (pH 6.8) to a 96-well plate at 50 μl / well, add an enzyme solution at 25 μl / well, and further add a substrate solution at 25 μl / well at 37 ° C. After incubation for 60 minutes, the absorbance at 475 nm was measured.

The inhibition of tyrosinase activity was tabulated by the inhibition rate obtained from the following formula.
Tyrosinase activity inhibition rate (%) = {1− (A−B) / C} × 100
A: Absorbance at 475 nm of the test solution B: Absorbance at 475 nm of the test blank solution C: Absorbance at 475 nm of the target solution The results are shown in Table 1.

  From the results of Examples 1 to 3 and Comparative Examples 1 to 8, it is clear that a chamomile extract and a peach extract are combined at a mass ratio of 25:75 to 75:25, thereby producing a synergistic effect on the tyrosinase activity inhibitory action. It is. In addition, when the mass ratio of the chamomile extract and the peach extract is 40:60 to 60:40, it is clear that a remarkably excellent tyrosinase activity inhibitory effect is exhibited (see Example 2).

Claims (2)

  A tyrosinase activity inhibitor characterized by containing an extract of Asteraceae chamomile chamomile flower part and an extract of rose family Peach genus peach bud in a mass ratio of 25:75 to 75:25.   A food or drink comprising the tyrosinase activity inhibitor according to claim 1.