JP2019083773A - Inspection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To inspect one or more embodiments of the present invention in which a container containing a liquid such as a developing liquid or a sample liquid is destroyed to cause the liquid to leak and come into contact with the carrier in a housing containing a chromatography carrier. In the device for use, the problem that the liquid is difficult to be absorbed by the carrier is solved. An inspection device 1 according to one or more embodiments of the present invention includes a chromatography carrier 30 and a housing 10, and the housing 10 is a support portion for supporting containers 40, 40 containing a liquid L. A perforated incision portion 13 for perforating or incising the 14 and the containers 40, 40 supported by the support portion 14 and the carrier 30, and leaking the liquid L from the containers 40, 40 to a position in contact with the carrier 30. A feature of the carrier 30 is that a perforated incision portion 36A, which is more easily perforated or incised by the perforated incision portion 13, is formed in advance at a position in contact with the perforated incision portion 13 of the carrier 30. do. [Selection diagram] FIG. 4A

Description

  The present invention relates to a test device used for chromatography.

  Nucleic acid chromatography is a technology for developing and capturing and detecting a substance to be detected having a nucleic acid tag attached thereto that hybridizes with the nucleic acid immobilized on the carrier on a chromatography carrier on which nucleic acid is immobilized. A plurality of detection targets contained in one sample are fixed by fixing nucleic acids having different base sequences at different positions on the chromatography carrier and adding a nucleic acid tag specific to the nucleic acids to a plurality of detection targets. It is possible to detect the substance on one carrier. In addition to nucleic acid chromatography, there is also a chromatography technology that captures a substance to be detected on a chromatography carrier using an antigen-antibody combination that can form a specific bond or a ligand-receptor combination. It is widely used in the fields of molecular biology testing and genetic testing for medical and food testing.

  In the above-described chromatography, various liquids such as a sample liquid containing a substance to be detected and a developing liquid are used. When these liquids are removed from the container with an instrument such as a pipette and applied to a chromatography carrier, the liquids may scatter in the test environment, resulting in contamination that causes false positives. In addition, opening the container in the inspection environment may contaminate the liquid in the container.

  With a liquid such as a sample liquid or a developing liquid used for chromatography contained in a container, a part of the container is broken to leak the liquid and brought into contact with the chromatography carrier without using an instrument such as a pipette Devices are being developed.

For example, a device for detecting an analyte in an affinity bioassay disclosed in Patent Document 1 is
At least one transparent wall,
At least one test recess adapted to receive at least one test strip and arranged so that the test strip is visible through the transparent wall;
At least one cavity adapted to receive a container containing a fluid sample, said cavity being in fluid communication with said at least one test recess;
The container is pierced when the container is inserted into the cavity, and is disposed corresponding to the at least one cavity such that at least a portion of the sample is expelled from the container to reach the test recess And drilling means.

In addition, the completely sealed target nucleic acid amplification product high-speed inspection device disclosed in Patent Document 2 is
Including inner structure and outer box,
The internal structure includes a fixed box part and a pedestal part,
The fixed box part has corresponding holes, which are used to hold the washing solution pack and the PCR tube containing the amplification product,
The pedestal portion includes a cleaning solution storage unit having a liquid pack piercing needle, an amplification product storage unit having a cutter, a sealing partition, a glass fiber paper, and a test strip sealing portion having a test strip and a transparent window,
The outer box is characterized by including a lever cover, a fixed box pressure portion, a washing solution pack pressure portion and a transparent window.

JP-A-2015-512250 Japanese Patent Publication No. 2010-500009

  A device in which a part of a container containing the liquid is broken to leak the liquid and brought into contact with the chromatography carrier in a housing containing the chromatography carrier is described in Patent Documents 1 and 2, and the like.

  As a result of intensive investigations, the present inventor combined the container and the chromatography carrier together in the case where the container containing the liquid is partially destroyed to leak the liquid in the housing containing the chromatography carrier. It has been newly found that the liquid leaked from the container can be efficiently brought into contact with the carrier by piercing or incising. On the other hand, when the present inventors attempt to perforate or cut the container and the chromatography carrier together with a perforated incision such as a blade, the position of the chromatography carrier may be shifted and the chromatography carrier may not be perforated or cut. I found a new task of The inventor has also found a new problem that when the container is pressed against the perforated incision together with the chromatography carrier to perforate or cut, the chromatography carrier may become resistant and require a large force.

An inspection device according to an embodiment of the present invention is
A chromatography carrier, and a housing for containing the carrier,
The housing is
A support for supporting a container containing a liquid used for chromatography;
A container supported by a support and a perforated incision for perforating or incising the carrier and causing liquid to leak from the container to a position where the carrier contacts the carrier;
It is characterized in that a to-be-pierced incision which is easier to be pierced or cut by the perforated incision is formed at a position in contact with the perforated incision of the carrier than the other part of the carrier.

  In the inspection device according to this embodiment, the perforated incision is formed in advance at a position of the chromatography carrier in contact with the perforated incision, so that the container and the chromatography carrier are formed by the perforation incision such as the blade. When perforating or incising together, it is difficult to shift the position of the chromatography carrier relative to the perforation incision, and it is easy to perforate or incise the container and the chromatography carrier together. In addition, when perforating or incising the chromatography carrier and the container by the perforation / incision portion, the resistance by the chromatography carrier is small, so it is relatively relative to the case of using the chromatography carrier in which the perforated / perforated portion is not formed. It is easy to pierce or cut the container and the chromatography carrier together with a small force.

In a more preferable aspect of the above-mentioned inspection device,
The carrier is housed in the housing such that a portion of the carrier in which the perforated incision is formed is located between the support and the perforated incision.
In the support portion, with the container supported, from the first position where the container faces the perforated incision through the portion of the carrier, the container is perforated or cut by the perforated incision together with the portion of the carrier The container guiding portion for guiding the container is formed up to the second position.

  In the inspection device of this aspect, the liquid leaked from the container at the second position is further easily absorbed because it is in immediate contact with the chromatography carrier.

In another preferred embodiment of the above-mentioned inspection device:
The perforated incision is
Notches penetrating in the thickness direction of the carrier,
A groove formed in a part in the thickness direction of the carrier,
A hole penetrating in a thickness direction of the carrier, and
From perforations in which two or more selected from a notch penetrating in the thickness direction of the carrier, a groove formed in part of the thickness direction of the carrier, and a hole penetrating in the thickness direction of the carrier are arranged And at least one selected from the group consisting of

  A chromatography carrier on which these pierced incisions are previously formed is preferred because it is easy to pierce or cut the pierced incision by the pierced incision.

In another preferred embodiment of the above-mentioned inspection device:
The length in the direction along the perforated incision of the perforated incision is 1 mm or more.

  A chromatography carrier in which a perforated incision having a length of 1 mm or more is previously formed is preferable because it is easy to pierce or incise the perforated incision by the perforated incision.

In another preferred embodiment of the above-mentioned inspection device:
The carrier is pre-formed with one or more perforated incisions.

  A chromatography carrier on which one or more pierced incisions are pre-formed is preferred as it is easy to pierce or cut the pierced incision by the pierced incision.

  In the inspection device according to one embodiment of the present invention, the perforated incision is formed in advance in the portion of the chromatography carrier in contact with the perforated incision, so that the container and the chromatography carrier are formed by the perforated incision. When the perforations and incisions are performed together, the position of the chromatography carrier relative to the perforation incision is unlikely to shift, and the container and the chromatography carrier can be perforated or separated together. In addition, when perforating or incising the chromatography carrier and the container by the perforation / incision, it is easy to perforate or cut the container and the chromatography carrier together with a small force because the resistance by the chromatography carrier is small. .

It is an exploded perspective view of a device for inspection concerning an embodiment of the present invention. 1 is a perspective view of an inspection device according to an embodiment of the present invention. It is the perspective view seen from the lower part of the case upper member of the device for inspection which concerns on embodiment of this invention. It is a perspective view of a blade (perforation incision part) of a device for inspection concerning an embodiment of the present invention. In a device for inspection concerning an embodiment of the present invention, it is a cross section schematic diagram of a longitudinal direction in the state where a container was supported by a supporter of a case and it was made to position in the 1st position. In a device for inspection concerning an embodiment of the present invention, it is a cross section schematic diagram of a longitudinal direction in the state where a container was supported by a supporter of a case and it was made to position in the 2nd position. The device for an inspection which concerns on embodiment of this invention WHEREIN: It is a schematic diagram of the end surface of the transversal direction containing one of a container in the state which supported the container by the support part of the housing and it made it position in the 1st position. The device for an inspection which concerns on embodiment of this invention WHEREIN: It is a schematic diagram of the end surface of the transversal direction containing one of a container in the state which supported the container by the support part of the housing | casing and it made it position in the 2nd position. A device for examination according to a comparative example in which a perforated incision is not previously formed on a chromatography carrier, including one of the containers in a state where the container is supported by the support of the housing and positioned in the second position It is a schematic diagram of the end surface of a transversal direction. 1 shows the structure of a chromatography carrier that can be used in the present invention. (A) shows a plan view and (B) shows a side view. 2 shows the structures of other chromatography supports that can be used in the present invention. (A) shows a plan view and (B) shows a side view. The example of the chromatography support | carrier in which the notch | incision was formed as a to-be-perforated incision part is shown. (A) shows a plan view, (B) shows a cross-sectional view taken along the line I-I of (A). (C) shows the top view of the example in which the notch | incision is formed from the edge part of the chromatography support | carrier. The example of the chromatography support | carrier in which the groove | channel without a width | variety was formed as a to-be-pierced incision is shown. (A) shows a plan view and (B) shows a cross-sectional view taken along the line II-II of (A). The example of the chromatography support | carrier in which the groove | channel with a width | variety was formed as a to-be-perforated incision is shown. (A) shows a plan view, and (B) shows a cross-sectional view taken along line III-III of (A). The example of the chromatography support | carrier with which the perforation was formed as a to-be-perforated incision part is shown. (A) shows a plan view, (B) shows a cross-sectional view taken along line IV-IV of (A).

  Although specific embodiments of the present invention will be described below with reference to the drawings, the scope of the present invention is not limited to the illustrated embodiments.

  An inspection device 1 according to an embodiment will be described with reference to FIGS. 1A to 4B.

  The inspection device 1 of the present embodiment includes a housing 10 and a lid 20. The material which comprises the housing | casing 10 and the cover body 20 is not specifically limited, Materials, such as resin and glass, can be used suitably. In the inspection device of the present invention, the lid 20 provided in the inspection device 1 of the present embodiment is not an essential component as described later.

  The housing 10 internally defines an internal space 11 for housing the chromatography carrier 30.

  The housing 10 is also disposed in the internal space 11 and is used to pierce or cut the containers 40, 40 and to puncture the liquid L from the containers 40, 40 to a position where it contacts the chromatography carrier 30. Equipped with

  In the illustrated example, the housing 10 is formed by the housing upper member 10A and the housing lower member 10B. The housing lower member 10 </ b> B includes a bottom wall portion 101 of the housing 10 and a side wall portion 102 erected from the periphery of the bottom wall portion 101. The housing upper member 10 </ b> A includes an upper wall portion 103 of the housing 10, a support portion 14 to be described later, and a lid mounting portion 15. In the upper wall portion 103 of the housing upper member 10A, the surface that is the outer surface of the housing 10 when combined with the housing lower member 10B is the upper surface 103a, and the surface that is the inner surface of the housing 10 is the lower surface 103b. Four locking claws 10A1 protruding toward the lower surface 103b are formed on the peripheral edge of the upper wall 103 of the housing upper member 10A, and the outer surface 102a of the side wall 102 of the housing lower member 10B is formed. Four locking recesses 10B1 that engage with the locking claws 10A1 are formed.

  The upper end 102b of the side wall portion 102 of the lower case member 10B and the peripheral portion 103b1 of the lower surface 103b of the upper wall portion 103 of the upper case member 10A engage the upper case member 10A and the lower case member 10B. When they hit each other. Further, on the lower surface 103b of the upper wall portion 103, a protruding portion 103b2 is formed adjacent to the inner peripheral side along the peripheral edge portion 103b1 and protruding from the peripheral edge portion 103b1. When the housing upper member 10A and the housing lower member 10B are engaged, the outer peripheral surface 103b3 of the projecting portion 103b2 of the lower surface 103b of the upper wall portion 103 in the housing upper member 10A and the side wall in the housing lower member 10B Of the inner surface 102c of the portion 102, a portion adjacent to the upper end 102b abuts. With these configurations, when the housing 10 is formed by engaging the housing upper member 10A and the housing lower member 10B, liquid leakage from the boundary between the side wall portion 102 and the upper wall portion 103 can be prevented. .

  Although the case 10 is formed of two members (the case upper member 10A and the case lower member 10B) in the illustrated example, the invention is not limited thereto, and the case 10 is formed of one member. It may be formed of three or more members.

  The internal space 11 is a space contained in the housing 10, and in the illustrated example, it is in communication with the outside through the holes 12 and 12, but the other part is a closed structure. In the illustrated embodiment, the bottom wall portion 101, the side wall portion 102, and the upper wall portion 103 surrounding the internal space 11 constitute a main body portion of the housing 10.

  The support portion 14 supports one or more (two in the illustrated example) containers 40, 40 containing the liquid L used for chromatography, and the containers 40, 40 from the first position to the second position described later. Holes 12 and 12 are formed as container guiding portions for guiding. The holes 12, 12 function as guide holes for guiding the containers 40, 40 respectively inserted and supported therein from a first position to a second position described later. The container guiding portion may have any structure as long as it can guide the container from the first position to the second position described later, and is not limited to the holes 12 and 12 formed in the support portion 14. The holes 12 and 12 are through holes that communicate the internal space 11 of the housing 10 with the outside. The holes 12 and 12 may be capable of inserting and supporting the containers 40 and 40 in the penetrating direction and guiding the inserted containers 40 and 40 toward the piercing and cutting portion 13, and the shape is It is not limited to a specific shape. In addition, "S" drawn on the outer surface 14a of the support part 14 shown to FIG. 1A is a label | marker of being the hole 12 which penetrates the container 40 which accommodated the sample liquid (sample) as the liquid L, "B" Is a mark indicating that the hole 12 is for inserting a container 40 containing a developing liquid (buffer) as the liquid L.

  The internal space 11 of the housing 10 is a place for developing the chromatography. A chromatography carrier 30 which is a solid phase is disposed in the internal space 11. When the liquid L is in contact with part of the chromatography carrier 30 in the internal space 11, the liquid L is developed in the chromatography carrier 30, and chromatography is performed.

  Examples of the liquid L include a sample solution containing a substance to be detected in a solvent such as water, a developing solution, and a liquid containing a labeling substance in a solvent such as water. The solvent is typically water. As a sample solution, a sample solution containing a nucleic acid amplification product can be exemplified. Examples of the developing solution include phosphate buffer, Tris buffer, Good buffer, SSC buffer and the like. The developing solution may further contain a surfactant. The liquid L may be a mixed liquid in which one or more kinds of a sample liquid, a developing liquid, and a liquid containing a labeling substance are mixed. Also, as the liquid L, a liquid containing a coloring reagent or a dye may be used. In the drawings and the following description, the liquid contained in the plurality of containers 40 and the liquid discharged to the internal space 11 are all represented as the liquid L, but the liquids L contained in the plurality of containers 40 are liquids of different compositions. May be there. Further, the container 40 for containing the liquid L other than the sample liquid may be inserted through the hole 12 in advance.

  The chromatography carrier 30 installed in the internal space 11 of the housing 10 may be any solid phase carrier, and may be made of resin, metal, polysaccharide, mineral or the like, and may be a membrane, film, non-woven fabric, plate , Gel and the like. In the illustrated example, the chromatography carrier 30 is an elongated membrane (strip) but is not limited to this example. Preferably, the chromatography carrier 30 has a porous structure. Specific examples of the chromatography carrier 30 include, for example, paper, nitrocellulose membrane, polyether sulfone membrane, nylon membrane, dried various gels (silica gel, agarose gel, dextran gel, gelatin gel), silicon, glass, resin, etc. Can be mentioned.

  In part of the chromatography carrier 30, a capture substance for capturing a detection target substance can be arranged. For example, when the substance to be detected contains a nucleic acid, a nucleic acid that hybridizes to the nucleic acid can be used as a capture substance, and when the substance to be detected contains an antigen or an antibody, immunology with the antigen or antibody A reactive antibody or antigen can be used as a capture substance.

  The substance to be detected is preferably one that can be visually detected on the chromatography carrier 30. For example, if a substance to be detected is labeled with a visually detectable labeling substance and developed on the chromatography carrier 30, visual detection is possible. Examples of labeling substances that can be detected visually include colored particles, dyes, and fluorescent substances. Examples of “colored particles” include metal (eg, gold, silver, copper, platinum etc.) particles, metal nanorods, metal nanoplates, latex particles containing dyes and fluorescent materials, silica nanoparticles including dyes and fluorescent materials, etc. Be The method of labeling the substance to be detected with a labeling substance capable of visual detection is not particularly limited. For example, when the substance to be detected contains a nucleic acid, the labeling substance to which the nucleic acid hybridizing to the nucleic acid is linked can be labeled with the labeling substance by bringing the substance to be detected into contact with the substance to be detected. For visual detection, at least a part of the wall portion of the case 10 surrounding the internal space 11 is formed of a material that transmits visible light so that the chromatography carrier 30 can be confirmed from the outside of the case 10 Is preferred. In particular, it is preferable that at least a portion 103c of the upper wall portion 103 of the housing 10 facing the portion where the chromatography carrier 30 is disposed be made of a material that transmits visible light.

  The structure of the chromatography carrier 30 used in the present embodiment is shown in FIG. In this example, the chromatography carrier 30 comprises a liquid receiver 31 and a detector 32. The liquid receiving portion 31 is disposed on one end side of the detecting portion 32, and the liquid L is supplied to this portion. The detection unit 32 is a portion in which a substance to be detected is developed. An absorption pad 33 is disposed on the other end side of the detection unit 32. Between the liquid receiving unit 31 and the detection unit 32, a labeling substance holding unit 34 for holding a labeling substance is disposed. When the detection target substance is contained in the liquid L supplied to the liquid receiving unit 31, the detection target substance is labeled by the labeling substance when passing through the labeling substance holding unit 34, and then moves to the detection unit 32. However, if the substance to be detected is labeled in advance, or if the liquid L contains a labeled substance, the labeled substance holding portion 34 is not necessary, and the liquid receiving portion 31 and the detecting portion 32 are adjacent to each other or The liquid receiver 31 can be omitted. The detection section 32 is formed with a capture area 321 in which the above-mentioned capture substance is disposed for capturing a detection target substance. The liquid receiving unit 31, the labeling substance holding unit 34, the detection unit 32, and the absorption pad 33 can be made of the above-described material as a material that can be used as the chromatography carrier 30. These may be comprised from the same member, and may be comprised from a different member. The liquid receiving unit 31, the labeled substance holding unit 34, the detection unit 32, and the absorption pad 33 can be disposed on the substrate 35 as illustrated. The substrate 35 can be made of, for example, a resin, metal, polysaccharide, mineral or the like. The substrate 35 can be partially or completely omitted. For example, by partially or completely omitting the substrate corresponding to the lower portion of the liquid receiving portion 31, the flexibility of the liquid receiving portion 31 is improved, and the resistance when pushing the container 40 toward the perforated incision is reduced. can do. Similar effects can be obtained by using a highly flexible substrate. The chromatography carrier 30 is not limited to one having the structure shown in FIG. 6, and one having an appropriate structure can be used according to the target chromatography.

  As another example of the chromatography carrier 30, as shown in FIG. 7, a chromatography carrier 30 comprising only the detection unit 32 without the liquid receiving unit 31, the labeled substance holding unit 34, the absorption pad 33, and the substrate 35. Can be mentioned. Further, although not shown, it is also possible to use a chromatography carrier 30 consisting only of the detection unit 32 and the base material 35 supporting the same.

  In the present embodiment, when the chromatography carrier 30 includes the labeling substance holding unit 34 in the internal space 11 of the housing 10, the liquid receiving unit 31 contacts the liquid L leaked from the container 40, but detection The portion 32 and the absorbent pad 33 are disposed so as not to be in direct contact with the leaked liquid L. As described above, when the liquid L contains a labeling substance, the labeling substance holding portion 34 can be omitted from the chromatography carrier 30. Further, as described above, it is also possible to use the chromatography carrier 30 consisting only of the detection unit 32, and the chromatography carrier 30 consisting only of the detection unit 32 and the base material 35. When the chromatography carrier 30 in which the labeling substance holding portion 34 is omitted, the chromatography carrier 30 consisting only of the detection portion 32 shown in FIG. 7, or the chromatography carrier 30 consisting only of the detection portion 32 and the base 35 The liquid L leaked from the container 40 may be disposed to be in direct contact with the detection unit 32.

  In the following description, the portion of the chromatography carrier 30 in direct contact with the liquid L leaked from the container 40 is referred to as a liquid contact portion 30L.

  In the internal space 11 of the housing 10, there is formed a carrier installation portion 16 which is erected from the bottom wall portion 101 into the internal space 11 and on which the chromatography carrier 30 is installed. The carrier setting unit 16 is between the bottom wall portion 101 and the upper wall portion 103 in the opposing direction of the bottom wall portion 101 and the upper wall portion 103 of the housing 10, and the upper wall portion 103 of the chromatography carrier 30. It is configured such that it can be installed so as to face the portion 103 c of the upper wall portion 103 at a closer position. The detection unit 32 of the chromatography carrier 30 and the absorption pad 33 are disposed on the upper surface 16 a of the carrier placement unit 16. On the other hand, the liquid contact portion 30L is disposed between the support portion 14 of the housing 10 and the perforation / incision portion 13. The detection unit 32 and the absorption pad 33 of the chromatography carrier 30 may be fixed to the upper surface 16 a of the carrier installation unit 16 by an adhesive or an adhesive tape. The upper surface 16 a of the carrier placement unit 16 is located at a position where the liquid L leaked from the container 40 pierced or cut by the perforated incision 13 does not reach, specifically, when the housing 10 is placed on a horizontal surface, In the internal space 11, it is at a position higher than the position where the liquid L is leaked. The upper surface 16a of the carrier placement unit 16 has a plan view shape corresponding to the portion including the detection unit 32 of the chromatography carrier 30 and the absorption pad 33, and in the illustrated example, has a shape having a longitudinal direction and a lateral direction. The carrier placement unit 16 is provided with a plurality of positioning protrusions at positions surrounding the top surface 16 a that restrict and position the movement of the chromatography carrier 30 in the direction along the top surface 16 a. The positioning projection includes a first positioning projection 16b disposed on both sides in the lateral direction of the upper surface 16a, and a second positioning projection 16c disposed on one longitudinal end of the upper surface 16a.

  Although the number of the holes 12 which are container guiding portions formed in the support portion 14 of the housing 10 is two in the illustrated example, it may be one or three or more, and it is used for the target chromatography It can be set according to the number of containers 40 containing the liquid L. For example, when using a sample solution and a developing solution as the liquid L, two holes 12 are formed. In this case, although not particularly limited, two holes 12, 12 are formed along the deployment direction at a position opposite to the position where the chromatography carrier 30 is disposed in the internal space 11, and the holes on the downstream side in the deployment direction 12, inserting the container 40 containing the sample liquid and inserting the container 40 containing the developing liquid into the hole 12 on the upstream side in the developing direction results in that the amount of the detection target substance reaching the detection unit 32 increases. preferable. Further, when the number of containers 40 is two or more, each container 40 may have a different shape, color tone, or pattern in order to easily distinguish the respective containers, and the liquid L contained in each container 40 is It may be colored to have different color tones. If two or more containers 40 having different shapes are used, each hole 12 may also have different shapes to fit each container shape.

  The lid 20 is attached to the housing 10 and configured to cover the support 14 of the housing 10. The lid 20 supports the support 14 so that when the liquid is present between the support 14 and the lid 20, the liquid does not leak to the outside of the inspection device 1 under normal use conditions. cover.

  In the present embodiment, the lid 20 includes the lid main portion 21 and the lid peripheral wall portion 22 starting from the outer peripheral edge of the lid main portion 21 and extending toward the inner surface 21 a of the lid main portion 21.

  At least one of the housing 10 and the lid 20 includes a lid guiding unit 50 for guiding the lid 20 to the housing 10.

  In the present embodiment, in the case 10, the support portion 14 is formed at a position projecting outward from the upper wall portion 103, and the support portion 14 and the upper wall portion 103 enclose the support portion 14 and a lid 20 are connected by a lid attachment unit 15 to be attached.

  In the present embodiment, the lid guiding portion 50 is formed on the housing side screwing portion 51 formed on the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall 22 of the lid 20. It is comprised from the formed housing side screwing part 51 and the lid side screwing part 52 which can be screwed. The lid 20 mounted on the housing 10 is rotated by the housing side screwing portion 51 and the lid side screwing portion 52 about the direction X in which the support portion 14 of the housing 10 and the lid 20 face each other as an axis. When moved, the lid 20 is guided by the lid guide 50 and moves in the direction X toward the support 14 of the housing 10.

  In order to make it easy to turn the lid 20, a plurality of recessed grooves 23a extending along the direction X are circumferentially spaced on the outer surface 22b of the lid peripheral wall 22 of the lid 20 as illustrated. It is preferable to form the non-slip portion 23 formed by vacancy. In addition, on the outer surface 21b of the lid main portion 21 of the lid 20, it is possible to form a marker 21c that allows visual identification of the rotational direction.

  The lid guiding portion 50 is not limited to the screwing portion illustrated, but may be in another aspect. For example, the screw attachment portion is not formed on the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall portion 22 of the lid 20. The aspect which the lid 20 slides and is guided with respect to the housing | casing 10 can be illustrated as another aspect, while the outer peripheral surface 15a of this and the internal peripheral surface 22a of the lid peripheral wall part 22 of the lid 20 contact | abut. Further, no screwing portion is formed on the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall portion 22 of the lid 20, and the lid mounting portion 15 of the housing 10 The cover 20 is guided relative to the housing 10 while the outer peripheral surface 15a of the cover 20 is in contact with the inner peripheral surface 22a of the cover peripheral wall 22 of the cover 20 via one or more sealing materials. It can illustrate as an aspect. The sealing material can be formed of an elastically deformable material such as rubber. Each sealing material may be fixed to one of the outer peripheral surface 15 a of the lid mounting portion 15 of the housing 10 and the inner peripheral surface 22 a of the lid peripheral wall 22 of the lid 20.

  The perforation / incision portion 13 pierces or cuts the containers 40, 40 supported by the support 14 together with the chromatography carrier 30, and positions the liquid L from the containers 40, 40 to be in contact with the liquid contact portion 30L of the chromatography carrier 30. Leak. In the present embodiment, a blade for cutting the containers 40, 40 (shown as "blade 13" when pointing to a mode specified as the blade of the perforated incision 13) is used as the perforated incision 13 in the present embodiment. The blade 13 shown in FIG. 2 is a plate-like blade in which the blade edge part 131 is formed on a part of the sides and the base end part 134 is formed on the other side. In the vicinity of the blade edge portion 131, the two side surfaces 132 and 133 are both blades which intersect at the blade edge portion 131 while being inclined together, but this is not limited thereto. Only one of the two side surfaces 132 and 133 is inclined It may be a single-edged blade intersecting at the cutting edge portion 131 while being flat. In the illustrated embodiment, the blade 13 is a rectangular plate-like blade, but plate-like blades of other shapes such as parallelograms, trapezoids, triangles, circles and the like can also be suitably used. Also, the piercing and cutting part 13 is not limited to the blade. Examples of the perforated incision 13 other than the blade include a needle that pierces the containers 40 and 40 together with the chromatography carrier 30. In the present embodiment, the plurality of containers 40, 40 together with the chromatography carrier 30 are configured to be incised by one perforated incision 13. However, the present invention is not limited to this. The part 13 may be provided. The material constituting the perforation / incision portion 13 may be any material capable of giving the container 40, 40 together with the chromatography carrier 30 a strength capable of perforation or dissection, for example, metals such as steel, stainless steel, aluminum, ceramics, It can be a resin.

  The perforated incision 13 is disposed in the interior space 11 of the housing 10. In the present embodiment, the perforation / incision portion 13 is disposed and fixed in the portion of the bottom wall portion 101 which is a wall portion facing the holes 12 and 12 in the internal space 11 of the housing 10 It is not limited. At a part of the bottom wall portion 101, a fixing portion 17 for fixing the perforated incision 13 is formed. In the present embodiment, the fixing portion 17 fixes the proximal end portion 134 of the blade 13 which is a perforated incision so as to sandwich the blade 13 from both sides in the thickness direction, and the blade edge portion 131 of the blade 13 It is arranged to face the support 14 inside. In the present embodiment, the fixing portion 17 is arranged on the bottom wall portion 101 so as to be aligned with the carrier mounting portion 16. When the inspection device 1 of the present embodiment is placed on a horizontal surface, the upper surface 17 a of the fixing portion 17 is located lower than the upper surface 16 a of the carrier installation portion 16. The carrier setting portion 16 and the fixing portion 17 are connected by the connection portion 18 provided upright in the internal space 11 from the bottom wall portion 101, and the upper surface 16 a of the carrier setting portion 16 and the upper surface 17 a of the fixing portion 17 Are connected by the upper surface 18 a of the connecting portion 18 inclined from the upper surface 16 a of the carrier mounting portion 16 to the upper surface 17 a of the fixed portion 17. A third positioning projection 17b is further formed on both sides of the upper surface 17a in the short direction of the fixing portion 17 for fixing the perforation / incision portion 13. As the containers 40 and 40 are guided and moved from the first position to the second position described later, the chromatography carrier 30 disposed between the perforated incision 13 fixed to the fixing portion 17 and the holes 12 and 12. When the liquid contact portion 30L moves toward the fixed portion 17, the movement of the liquid contact portion 30L is restricted in the movement direction of the containers 40, 40.

  The container 40 contains a liquid L used for chromatography, can be inserted into and supported by the holes 12 and 12 of the housing 10, and can be perforated or cut by the perforation / incision portion 13 be able to. The container 40 is typically a resin container. As the container 40, a tubular container extending in one direction and having one end closed and an open / close portion at the other end is preferable. Specifically, as illustrated, when supported by the support 14, the outer side of the housing 10 is provided. It is possible to use a container having an opening and closing portion 41a at an outer end 41 which is an end of the container 10 and an inner end 42 which is an inner end of the housing 10 is closed. The container 40 provided with the opening and closing part 41a at the outer end 41 as shown in the drawing is a hinge part 401 and a knob part 402 which are projected outward at the periphery 400 of the opening and closing part 41a. And, as such a container 40, a microtube used for a nucleic acid amplification reaction or a biochemical test can be exemplified.

  The container 40 to be inserted and supported is urged toward the axial center along the penetrating direction of each hole 12 at a position surrounding each of the holes 12 of the support portion 14, and the inside of each hole 12 of the container 40 is An elastic member 190 is disposed to maintain the posture of the seat. Specifically, the elastic member 190 is a pair of the inner surface 14b on the side of the inner space 11 of the support portion 14, which protrudes toward the inner space 11 from a pair of opposing positions around the holes 12 The elastic support pieces 191 and 192 of FIG. The pair of elastic support pieces 191 and 192 is disposed such that the surface 191 a of one elastic support piece 191 and the surface 192 a of the other elastic support piece 192 face each other. The surface 191 a of one elastic support piece 191 and the surface 192 a of the other elastic support piece 192 are formed to be closer as the distance along the direction X from the inner surface 14 b of the support portion 14 increases. The pair of elastic support pieces 191 and 192 sandwiches the container 40 inserted in each hole 12 from the side, and biases the container 40 toward the axial center along the penetrating direction of each hole 12 to maintain the posture. The pair of elastic support pieces 191, 192 are elastically deformable and are pushed open following the shape of the container 40 as the container 40 is pushed toward the perforated incision 13 as shown in the figure. Even in the case of using the container 40 having a shape in which the width is increased from the end to the outer end 41, the posture of the container 40 can be stabilized. In addition, if the elastic support pieces 191 and 192 are in the elastically deformable range, the containers 40 having different widths can be supported and stabilized. In the present embodiment, the position of the container 40 with respect to the perforated incision 13 is stabilized by holding the posture of the container 40 inserted in each hole 12 by the elastic member 190 including the pair of elastic support pieces 191 and 192. And the amount of liquid L leaked from the container 40 can be stabilized.

  Subsequently, referring to FIGS. 3A to 4B, a mechanism for perforating or incising containers 40 and 40 inserted and supported in holes 12 and 12 of support 14 and chromatography carrier 30 by perforation and dissection 13. Will be explained.

  FIGS. 3A and 4A show a state in which the containers 40 and 40 containing the liquid L are inserted into and supported by the holes 12 and 12 of the support portion 14. At this time, the inner ends 42 and 42 of the containers 40 and 40 face the cutting edge portion 131 of the blade 13. Thus, the container is supported by the support, and the position of the container in a state where one end of the container faces the perforated incision is taken as the "first position".

  The containers 40, 40 supported in the holes 12, 12 of the support portion 14 and positioned at the first position are pushed toward the perforated incision 13 and the inner ends 42, 42 of the containers 40, 40 The fully moved state until it abuts on 17 is shown in FIGS. 3B and 4B. At this time, the containers 40, 40 are cut by the blade 13 from the side of the inner ends 42, 42 of the containers 40, 40. Thus, the container is supported by the support portion, guided by the container guide portion, and the position of the container in the state of being moved until it is pierced or cut by the piercing and cutting portion is set to the “second position”. As shown in FIGS. 3B and 4B, in the present embodiment, the container in the second position is in contact with a portion of the housing other than the perforated incision.

  In the process of guiding the containers 40, 40 supported by the holes 12, 12 of the support portion 14 from the first position to the second position, the containers 40, 40 are pierced or cut by the perforation / incision portion 13. , 40 leaks to a position where it comes into contact with the liquid contact portion 30L of the chromatography carrier 30. The containers 40, 40 are pierced or cut by the piercing section 13 to form the leak ports 43, 43. From the containers 40, 40 in which the leak ports 43, 43 are formed, the stored liquid L leaks into the internal space 11 through the leak ports 43, 43, and not only the liquid contact portion 30L of the chromatography carrier 30 , In contact with a portion of the housing 10 including the perforated incision 13 and the bottom wall 101 (especially the fixing portion 17) around the periphery thereof.

  Further, in the present embodiment, the containers 40, 40 are accommodated in the housing 10 such that the liquid contact portion 30L of the chromatography carrier 30 is positioned between the support portion 14 and the perforation / incision portion 13. It is characterized in that it is perforated or cut out by the perforated incision 13 together with the chromatography carrier 30. When the containers 40, 40 are in the first position, the inner ends 42 of the containers 40, 40 and the piercing incision 13 are opposed via the liquid contact portion 30L of the chromatography carrier 30 therebetween. When the containers 40, 40 supported by the support portion 14 are guided and moved from the first position to the second position, the containers 40, 40, together with the liquid contact portion 30L of the chromatography carrier 30, are pierced by the piercing incision 13 Or incised. According to this embodiment, it is easy for the liquid L leaked from the containers 40 and 40 to contact and permeate the liquid contact portion 30L of the chromatography carrier 30. The detection device 1 having this feature is preferable because the liquid L leaked from the containers 40 can be efficiently brought into contact with the chromatography carrier 30.

  In the present embodiment, as shown in FIG. 3A and FIG. 4A, with the containers 40 and 40 supported by the support portion 14 of the housing 10 and positioned at the first position, the lid 20 is the housing 10. It mounts to the lid mounting part 15 which encloses the support part 14 of this. At this time, the housing side screwing portion 51 on the outer peripheral surface 15a of the lid mounting portion 15 of the housing 10 and the lid side screwing portion 52 on the inner peripheral surface 22a of the lid peripheral wall portion 22 of the lid 20 Screw it together. Thereby, the support portion 14 of the housing 10 and the outer portions 40 a and 40 a of the containers 40 and 40 are covered by the lid 20. Subsequently, when the lid 20 is rotated about the direction X in which the support 14 of the housing 10 and the lid 20 face each other, the lid 20 is guided by the lid guiding portion 50, It moves toward the support 14 of the housing 10 along X. In the process of this movement, the lid main portion 21 of the lid 20 abuts on the outer ends 41 of the containers 40, 40, whereby the containers 40, 40 are pushed toward the perforated incision 13 as shown in FIG. The second position shown in FIG. 4B is reached. When the lid 20 is rotated about the direction X in which the support 14 of the housing 10 and the lid 20 face each other, the container 40 is slightly inclined from the direction X by the rotation of the lid 20. It is pushed into the piercing incision 13. By this action, the leakage port 43 formed by perforation or incision of the containers 40, 40 is opened, so that the liquid L is effectively leaked, and the chromatography conditions can be stabilized.

  The inspection device of the present invention is not necessarily provided with a lid as in this embodiment. For example, the inspection device does not include the lid, and the user presses the container supported by the support portion of the housing from the outside of the housing using an external force by operating the fingertip, the device, and the like. The container may be configured to move from the first position to the second position.

  In the inspection device 1 of the present embodiment, at least a part of the outer surface 102 a of the side wall portion 102 corresponding to the side surface of the housing 10 is formed with the wavelike surfaces 102 d and 102 d. The wavy surfaces 102d and 102d are surfaces in which a plurality of grooves 102e extending in a direction in which the bottom wall portion 101 and the upper wall portion 103 of the housing 10 face each other are continuously formed. By forming the wavelike surfaces 102 d and 102 d on the outer surface 102 a of the side wall portion 102, slippage can be prevented when the user handles the inspection device 1 with his / her hand. In addition, when preparing a plurality of inspection devices 1 of the present embodiment and arranging so that the outer surface 102 a of the side wall portion 102 of the housing 10 is in contact with each other, the waved surfaces 102 d can be engaged with each other. The wavy surface is an example of the uneven surface, and in the inspection device 1 of this embodiment, the wavy surface 102d may be replaced with an uneven surface having another shape. The uneven surface is a surface in which a plurality of grooves extending in one direction are continuously formed, and a flat surface may be formed between adjacent grooves. The shape of the cross section by a plane perpendicular to the extending direction of each of the plurality of grooves in the uneven surface is not particularly limited. Although the case where the shape of the cross section is a curved line is a wavelike surface as illustrated, the shape of the cross section may be a rectangle, a U-shape, or the like.

  The inspection device 1 according to the present embodiment is a portion of the chromatography carrier 30 which is in contact with the perforation / incision portion 13 more easily to be perforated or incised by the perforation / incision portion 13 than the other portions of the chromatography carrier 30. An incision is formed in advance.

  As a specific example of a perforated incision, in the embodiment shown in FIGS. 1A to 4B, the liquid contact portion 30L of the chromatography carrier 30 is formed with a slit hole 36A penetrating in the thickness direction of the chromatography carrier 30. . The slit hole 36A is formed in a shape corresponding to the cutting edge portion 131 of the blade 13 which is a perforated cutting portion, that is, a shape extending along the cutting edge portion 131. In the inspection device 1 of the present embodiment in which the chromatography carrier 30 in which the slit hole 36A is formed in the liquid contact portion 30L is housed in the housing 10, as shown in FIGS. 3A and 4A, the containers 40, 40 are used. The liquid contact portion 30L of the chromatography carrier 30 is supported by the support portion 14 of the housing 10 at the first position, and the liquid contact portion 30L of the chromatography carrier 30 includes the inner ends 42, 42 of the containers 40, 40 and the blade 13 opposed thereto. Between the cutting edge 131 of the Then, the slit hole 36A is positioned at a position in the liquid contact portion 30L of the chromatography carrier 30 in contact with the cutting edge portion 131 of the blade 13. Subsequently, when the lid 20 is rotated with respect to the housing 10 around the direction X, the lid 20 is guided by the lid guiding unit 50 and supports the housing 10 along the direction X. Move towards section 14 In the process of this movement, the containers 40, 40 together with the liquid contact portion 30L of the chromatography carrier 30 are pushed toward the blade 13 by the lid main portion 21 of the lid 20, and finally, as shown in FIGS. 3B and 4B. It reaches the second position shown in. In the present embodiment, when the containers 40, 40 move from the first position to the second position, the cutting edge portion 131 of the blade 13 is easier to cut the slit hole 36A than the other portions of the chromatography carrier 30. Therefore, as intended, the container 40, 40 is cut through the portion of the slit hole 36A of the chromatography carrier 30. As described above, in the present embodiment, the slit holes 36A are formed in advance at the position in contact with the blade 13 of the chromatography carrier 30, so that the containers 40 and 40 and the chromatography carrier 30 are put together by the blade 13. It is difficult to shift the position of the chromatography carrier 30 relative to the blade 13 when making an incision, and it is easy to cut the containers 40, 40 and the chromatography carrier 30 together. In addition, when cutting with the blade 13, the resistance by the chromatography carrier 30 is small, so a relatively small force is used as compared to the case of using the chromatography carrier 30 in which a perforated incision such as the slit hole 36A is not formed. It is easy to cut the containers 40 and 40 and the chromatography carrier 30 together.

  FIG. 5 shows an inspection device 1 ′ of a comparative example having the same configuration as that of the above embodiment except that a perforated incision such as a slit hole 36A is not formed in the liquid contact portion 31L of the chromatography carrier 30. The state when using the container 40, 40 is supported by the support part 14 and it guides from a 1st position to a 2nd position is shown typically. In the inspection device 1 ′ of the comparative example, when the containers 40 and 40 and the chromatography carrier 30 are cut together by the blade 13, the position of the chromatography carrier 30 with respect to the blade 13 easily shifts. As a result, as illustrated in FIG. 5, only the containers 40 and 40 are incised without the liquid contact portion 30L of the chromatography carrier 30 being cut at the intended portion, and the liquid contact portion 30L of the chromatography carrier 30 is There is a possibility that the liquid L which has leaked from the containers 40, 40 can not be sufficiently contacted.

  In the embodiment shown in FIGS. 1A to 4B, the chromatography carrier 30 is formed with the slit hole 36A penetrating in the thickness direction of the chromatography carrier 30 as a specific example of the perforated incision. Is not limited.

In the present invention, the perforated incision is
Incisions through the thickness of the chromatography carrier,
A groove formed in a part in the thickness direction of the chromatography carrier,
Holes penetrating in the thickness direction of the chromatography carrier, and
Two or more selected from a notch penetrating in the thickness direction of the chromatography carrier, a groove formed in a part in the thickness direction of the chromatography carrier, and a hole penetrating in the thickness direction of the chromatography carrier are arranged One or more selected from the group consisting of perforations is preferred. The slit holes 36A in the embodiment shown in FIGS. 1A to 4B are one aspect of the holes penetrating in the thickness direction of the chromatography carrier.

  FIG. 8 illustrates an example in which a notch 36 B penetrating in the thickness direction of the chromatography carrier 30 is formed at a position in contact with the perforated incision 13 of the chromatography carrier 30. The cut 36B may be formed so that the whole thereof is located inside the chromatography carrier 30 in a plan view as shown in FIG. 8 (A), as shown in FIG. 8 (C). , The end of the chromatography carrier 30 may be formed. Also, although not shown, other forms of pierced incisions may also be formed from the end of the chromatography carrier.

  FIG. 9 illustrates an example in which a non-width groove 36C formed in a part in the thickness direction of the chromatography carrier 30 is formed at a position in contact with the perforated incision 13 of the chromatography carrier 30.

  FIG. 10 illustrates an example in which a wide groove 36D formed in a part in the thickness direction of the chromatography carrier 30 is formed at a position in contact with the perforated incision 13 of the chromatography carrier 30.

  As shown in FIG. 9 and FIG. 10, the surface of the chromatography carrier 30 on the side where the groove 36C without width or the groove 36D with width is formed is referred to as a surface 30A. It is preferable from the viewpoint of positional deviation prevention that the perforation / incision portion 13 is disposed to be in contact with the chromatography carrier 30 from the side of the surface 30A.

  FIG. 11 illustrates an example in which a perforation 36E in which five round holes 36E1 penetrating in the thickness direction of the chromatography carrier 30 are arranged is formed at a position in contact with the perforated incision 13 of the chromatography carrier 30. .

  The length in the direction along the perforated incision of the perforated incision (length A shown in FIGS. 8 to 11) is preferably 1 mm or more, more preferably 2 mm or more, and more preferably 3 mm or more, More preferably, it is 5 mm or more. The length in the direction along the perforated incision of the perforated incision is preferably 0.3 times or more the length of the cutting edge 131, when the perforated incision is a plate-like blade 13 as shown in FIG. More preferably, it is 0.5 times or more, more preferably 0.8 times or more, more preferably 1.0 times or more. When the length in the direction along the perforated incision of the perforated incision is in the above-mentioned range, the chromatography carrier is easily pierced or dissected particularly in the perforated incision, and thus the above-mentioned effects of the present invention are easily exhibited.

  The number of perforated incisions formed in the portion of the chromatography carrier in contact with the perforated incisions is not particularly limited as long as it is one or more.

  The inspection device 1 of the above embodiment of the structure illustrated in FIGS. 1A to 4B was produced. As the chromatography carrier 30 (cut +), the liquid receiving portion 31, the labeled substance holding portion 34, the detecting portion 32, and the absorption pad 33 are disposed on the base material 35 having the structure shown in FIG. In addition, instead of the slit holes 36A, a chromatography carrier 30 in which a cut 36B having a length of 5 mm in the direction of development was formed as shown in FIG. 8A was used. As the chromatography carrier 30 (cut-), one having the same structure as the chromatography carrier 30 (cut +) was used except that the cut 36B was not formed.

  When the container 40 is supported by the support 14 of the housing 10 of the inspection device 1, the container 40 has an open / close portion 41 a at the outer end 41 which is the outer end of the housing 10. A microtube for biochemical testing was used, with the inner end 42 at the end closed.

  The container 40 containing 70 μL of the developing solution for chromatography L is supported by the support 14 of the case 10 so that the inner end 42 is directed downward in the support 14 of the case 10 of the inspection device 1. The cover 20 is not used, and it is moved from the first position to the second position by pushing the blade 13 from the side of the outer end 41 toward the blade 13, and the developing solution L is leaked to the chromatography carrier 30 for development. The At this time, the maximum value of the vertical force (maximum pressing force) required when pushing the container 40 was measured. The measurement was performed using a digital force gauge ZTA-200N (IMADA) and a measurement stand HV-500N II (IMADA). Ten measurements were performed for each of "incision +" and "incision-", and the average value and the standard deviation were calculated. The results are shown in Table 1. It was confirmed that the provision of the cut 36B in the chromatography carrier 30 significantly reduces the maximum pressing force.

  Furthermore, in the same procedure, the container 40 is supported by the support portion 14 of the housing 10, moved from the first position to the second position, and after 10 minutes from the movement to the second position, the chromatography carrier 30 is absorbed. It was evaluated whether the developing liquid L was developed on the pad 33 or not. When the developing solution L was developed to the absorbent pad 33, it was determined that the development was successful. 50 tests were conducted for each of “cut +” and “cut −”, the number of successful deployments was recorded, and the success rate of deployment was calculated. The results are shown in Table 2. It was confirmed that the success rate of the development is enhanced by providing the cut 36B in the chromatography carrier 30.

1: Inspection device 10: Case 12: Hole (container guide portion)
13: Perforated incisions 36A, 36B, 36C, 36D, 36E: Perforated incision 14: Support 30: Chromatography carrier 40: Container L: Liquid

Claims (5)

A testing device,
A chromatography carrier, and a housing for containing the carrier,
The housing is
A support for supporting a container containing a liquid used for chromatography;
A container supported by a support and a perforated incision for perforating or incising the carrier and causing liquid to leak from the container to a position where the carrier contacts the carrier;
The inspection device according to the present invention, wherein a to-be-pierced incision which is easier to be pierced or cut by the piercing incision than the other part of the carrier is formed in advance at a position contacting the piercing incision of the carrier. The carrier is housed in the housing such that a portion of the carrier in which the perforated incision is formed is located between the support and the perforated incision.
In the support portion, with the container supported, from the first position where the container faces the perforated incision through the portion of the carrier, the container is perforated or cut by the perforated incision together with the portion of the carrier The inspection device according to claim 1, wherein a container guiding part for guiding the container is formed up to a second position. The perforated incision is
Notches penetrating in the thickness direction of the carrier,
A groove formed in a part in the thickness direction of the carrier,
A hole penetrating in a thickness direction of the carrier, and
From perforations in which two or more selected from a notch penetrating in the thickness direction of the carrier, a groove formed in part of the thickness direction of the carrier, and a hole penetrating in the thickness direction of the carrier are arranged The inspection device according to claim 1 or 2, which is one or more selected from the group consisting of   The inspection device according to any one of claims 1 to 3, wherein a length in a direction along a perforated incision of the perforated incision is 1 mm or more.   5. The examination device according to any of the preceding claims, wherein the carrier is pre-formed with one or more perforated incisions.

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