JP2018112657A - microscope - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a microscope that can prevent the direction of a shadow of a specimen from being changed even when a stage is rotated.SOLUTION: A microscope 2 comprises: an objective lens 23; a stage 20 that is rotatable centering an optical axis of the objective lens 2 and on which a specimen SP is placed; a ring illumination part 25 that has a plurality of lighting parts arranged annularly centering the objective lens 23 and emits illumination light with which the specimen SP is irradiated; an eyepiece part 26 for observing an observation image of the specimen SP condensed by the objective lens 23; an operation device 3 that receives an input of a lighting signal indicating lighting positions of the plurality of lighting parts; and a microscope control part 28 that turns on the plurality of lighting parts on the basis of the lighting signal input from the operation device 3. When the stage 20 is rotated, the microscope control part 28 moves the lighting positions of the plurality of lighting parts following the rotation of the stage 20.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a microscope for observing an observation image formed by irradiating illumination light obliquely to a specimen and condensing the light reflected by the specimen to form an image.

  Conventionally, in microscopes, an oblique illumination unit with a plurality of lighting units arranged in an annular shape around the optical axis of the objective lens has been provided to irradiate the sample with light obliquely and collect scattered light scattered by the sample. A technique for observing an observation image formed in this way is known (see Patent Document 1). In this technique, a user operates a hand switch to change a light emission area of the oblique illumination unit, thereby observing minute scratches or irregularities on the specimen.

JP 2005-227442 A

  However, in Patent Document 1 described above, in the case where observation is performed with the orientation of the specimen and the illumination direction of the illumination light with respect to the specimen being adjusted, the direction of the shadow of the specimen changes when the stage is rotated. Again, there was a problem that the illumination direction of the illumination light with respect to the specimen had to be adjusted. Specifically, in Patent Document 1 described above, as shown in FIG. 20A, in the case where the user observes with the orientation of the specimen SP and the orientation of the illumination direction R1 of the illumination light with respect to the specimen SP being adjusted. For example, when the rotary stage 100 is rotated 45 degrees counterclockwise as shown in FIG. 20B, the direction of the shadow of the specimen SP changes (FIG. 20A → FIG. 20B), and the illumination light for the specimen SP again. There was a problem that the illumination direction had to be adjusted.

  The present invention has been made in view of the above, and an object of the present invention is to provide a microscope that can prevent the direction of the shadow of a specimen from changing even when the stage is rotated.

  In order to solve the above-described problems and achieve the object, a microscope according to the present invention includes an objective lens, a stage that can be rotated around the optical axis of the objective lens, and a specimen is placed thereon, and the objective A ring illumination unit 25 having a plurality of lighting units arranged in an annular shape around the lens and emitting illumination light for illuminating the sample, and an observation image of the sample collected by the objective lens An eyepiece unit for receiving, an input unit that receives an input of a lighting signal that indicates lighting positions of the plurality of lighting units, and the lighting unit that is input from the input unit, and that corresponds to the lighting position A microscope control unit that lights a plurality of lighting units, and the microscope control unit rotates the lighting positions of the plurality of lighting units following the rotation of the stage when the stage rotates. And

  The microscope according to the present invention further includes a drive unit that rotates the stage in the above invention, and the input unit is capable of receiving an input of a rotation signal that indicates a rotation amount of the stage, and the microscope control When the rotation signal is input from the input unit, the unit rotates the stage so that the rotation amount becomes the rotation amount, and sets the lighting positions of the plurality of lighting units based on the rotation amount. It is made to rotate.

  The microscope according to the present invention further includes a detection unit that detects a rotation amount of the stage in the above invention, and the microscope control unit includes the plurality of lighting units according to the rotation amount detected by the detection unit. The lighting position is moved.

  Further, in the microscope according to the present invention, in the above invention, the detection unit detects an amount of rotation of the stage, converts it into a pulse number and outputs the encoder, and a scale that detects and outputs the rotation angle of the stage. It is either one.

  The microscope according to the present invention further includes a drive unit that rotates the stage in the above invention, and the input unit is capable of receiving an input of a rotation signal that indicates a rotation amount of the stage, and the microscope control When the rotation signal is input from the input unit, the unit causes the drive unit to rotate the stage so that the rotation amount is obtained.

  The microscope according to the present invention records ring illumination rotation amount information in which the drive unit that rotates the stage and the rotation amount of the ring illumination unit 25 corresponding to the rotation amount of the stage are associated with each other in the above-described invention. A recording unit, wherein the input unit can accept an input of a rotation signal that indicates the amount of rotation of the stage, and the microscope control unit receives the rotation signal from the input unit, The stage is rotated by the driving unit so as to be the rotation amount, and the lighting positions of the plurality of lighting units are rotated based on the rotation amount and the ring illumination rotation amount information.

  Further, in the microscope according to the present invention, in the above invention, the ring illumination rotation amount information includes presence / absence information related to the presence / absence of lighting of a lighting unit adjacent to the rotation direction of the plurality of lighting units. .

  Further, in the microscope according to the present invention, in the above invention, the input unit inputs an invalid signal for invalidating a function for rotating the plurality of lighting units following the rotation of the stage or an effective signal for validating. The microscope control unit switches the function according to the invalid signal or the valid signal.

  According to the microscope of the present invention, there is an effect that it is possible to prevent the direction of the shadow of the sample from changing even when the stage is rotated.

FIG. 1 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 1 of the present invention. FIG. 2 is a diagram schematically showing a schematic configuration of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 3 is a flowchart showing an outline of processing executed by the microscope system according to Embodiment 1 of the present invention. FIG. 4A is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 4B is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 4C is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 4D is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 4E is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 4F is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 4G is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 1 of the present invention. FIG. 5A is a diagram schematically showing the direction of the shadow of the sample before the stage is rotated according to Embodiment 1 of the present invention. FIG. 5B is a diagram schematically showing the direction of the shadow of the sample after the stage is rotated according to Embodiment 1 of the present invention. FIG. 6 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 2 of the present invention. FIG. 7 is a top view schematically showing the configuration of the detection unit according to Embodiment 2 of the present invention. FIG. 8 is a flowchart showing an outline of processing executed by the microscope system according to Embodiment 2 of the present invention. FIG. 9 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 3 of the present invention. FIG. 10 is a top view schematically showing the configuration of the detection unit according to Embodiment 3 of the present invention. FIG. 11 is a flowchart showing an outline of processing executed by the microscope system according to Embodiment 3 of the present invention. FIG. 12 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 4 of the present invention. FIG. 13: is a figure which shows the outline | summary of the ring illumination rotation amount information table which shows the ring illumination rotation amount information which the ring illumination rotation amount information recording part concerning Embodiment 4 of this invention records. FIG. 14 is a flowchart showing an outline of processing executed by the microscope system according to Embodiment 4 of the present invention. FIG. 15 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 5 of the present invention. FIG. 16 is a diagram showing an outline of a ring illumination rotation amount information table indicating ring illumination rotation amount information recorded by the ring illumination rotation amount information recording unit according to Embodiment 5 of the present invention. FIG. 17 is a top view schematically showing the light and darkness that occurs when the specimen is illuminated by the ring illumination unit. FIG. 18 is a flowchart showing an outline of processing executed by the microscope system according to Embodiment 5 of the present invention. FIG. 19A is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 19B is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 19C is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 19D is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 19E is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 19F is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 19G is a diagram schematically showing a lighting location of the lighting unit during rotation of the ring illumination unit according to Embodiment 5 of the present invention. FIG. 20A is a top view schematically showing the direction of the shadow of the sample when the illumination light is irradiated to the sample placed on the stage before the rotation of the conventional stage. FIG. 20B is a top view schematically showing the direction of the shadow of the specimen when the specimen placed on the stage after the rotation of the conventional stage is irradiated with illumination light.

  DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments for carrying out the present invention (hereinafter referred to as “embodiments”) will be described with reference to the drawings. The present invention is not limited to the embodiments described below. The drawings referred to in the following description only schematically show the shape, size, and positional relationship so that the contents of the present invention can be understood. That is, the present invention is not limited only to the shape, size, and positional relationship illustrated in each drawing.

(Embodiment 1)
[Configuration of microscope system]
FIG. 1 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 1 of the present invention. In FIG. 1, a plane on which the microscope system 1 is placed is referred to as an XY plane, and a direction orthogonal to the XY plane is described as a Z direction.

  A microscope system 1 shown in FIG. 1 includes a microscope 2 that observes a specimen SP, an operating device 3 that receives input of an instruction signal instructing change of illumination light irradiated by the microscope 2 and change of observation magnification, and the microscope system 1. The control apparatus 4 which controls operation | movement, and the display apparatus 5 which displays the observation image of the sample SP by the microscope 2, and the various information of the microscope system 1 are provided.

[Configuration of microscope]
First, the configuration of the microscope 2 will be described.
The microscope 2 includes a stage 20, a drive unit 21, a revolver 22, an objective lens 23, a microscope main body unit 24, a ring illumination unit 25, an eyepiece unit 26, a recording unit 27, and a microscope control unit 28. .

  On the stage 20, a specimen SP is placed. The stage 20 has a disk shape and is disposed in the microscope main body 24 so as to be rotatable around an optical axis L1 of an objective lens 23 described later.

  The drive unit 21 rotates the stage 20 under the control of a microscope control unit 28 described later. The drive unit 21 is configured using, for example, a pulse motor.

  The revolver 22 is provided so as to be slidable or rotatable with respect to the microscope body 24, and the objective lens 23 is disposed above the specimen SP. The revolver 22 holds a plurality of objective lenses 23 having different magnifications (observation magnifications).

  The objective lens 23 is attached to the revolver 22. The objective lens 23 is disposed to face the stage 20. The objective lens 23 collects the light reflected by the sample SP. The magnification of the objective lens 23 can be changed as appropriate, for example, 10 times, 20 times, 100 times, or the like.

  The microscope main body 24 has a substantially C shape in side view. The microscope main body 24 movably supports the stage 20 and holds the objective lens 23 via the revolver 22.

  The ring illumination unit 25 irradiates the specimen SP with oblique illumination light (hereinafter simply referred to as “ring illumination”) from outside the center of the optical axis L 1 of the objective lens 23. The ring illumination part 25 has a plurality of lighting parts in an annular shape around the objective lens 23.

  FIG. 2 is a diagram schematically illustrating a schematic configuration of the ring illumination unit 25. As shown in FIG. 2, the ring illumination unit 25 is configured using a plurality of lighting units 251. The plurality of lighting portions 251 are arranged in an annular shape (ring shape) around the objective lens 23. The plurality of lighting units 251 are configured using light emitting LEDs (Light Emitting Diodes) or the like. For example, the lighting unit 251 is configured using a bullet-type LED. In addition, in place of the bullet-type LED, a chip-type LED having a wide directivity arranged on the chip may be used, but in the dark field observation, in order to collect and observe weak scattered light from the specimen SP, A bullet-type LED with narrow directivity is desirable. In addition, in this Embodiment 1, although the number of the lighting parts 251 of the ring illumination part 25 is demonstrated with 16 pieces, it is not limited to this, You may change the number of the lighting parts 251 suitably.

  The eyepiece 26 forms an observation image of the specimen SP collected by the objective lens 23. The user observes the observation image of the specimen SP through the eyepiece unit 26. Note that an imaging device capable of generating image data of an observation image of the specimen SP is connected to the observation side of the eyepiece 26 via an adapter, and an image corresponding to the image data generated by the imaging device is displayed on the display device 5. May be displayed.

  The recording unit 27 records various information related to the microscope 2 and various programs executed by the microscope 2. The recording unit 27 is configured using a nonvolatile memory, a volatile memory, or the like.

  The microscope control unit 28 drives the drive unit 21 based on the instruction signal input from the operation device 3 via the control device 4 to rotate the stage 20. Further, the microscope control unit 28 controls the lighting position, the number of lighting, and the lighting pattern of the lighting unit 251 of the ring illumination unit 25 based on the instruction signal input from the operation device 3 via the control device 4. The microscope control unit 28 is configured by using a CPU (Central Processing Unit), an ASIC (Application Specific Integrated Circuit), an FPGA (Field Programmable Gate Array), and the like, and reads various programs recorded in the recording unit 27. Execute.

[Configuration of operation device]
Next, the configuration of the controller device 3 will be described.
The controller device 3 receives an input of an instruction signal instructing lighting of the ring illumination unit 25. The operation device 3 is configured using an operation device having a mouse, a keyboard, buttons, and dial switches. In the first embodiment, the operating device 3 is connected to the microscope 2 via the control device 4, but is not limited to this, and may be directly connected to the microscope 2. In the first embodiment, the controller device 3 functions as an input unit.

[Configuration of control device]
Next, the configuration of the control device 4 will be described.
The control device 4 comprehensively controls the operation of each part constituting the microscope system 1. The control device 4 transmits various instruction signals received by the operation device 3 to the microscope 2. The control device 4 is configured using a CPU and a recording medium such as a nonvolatile memory or a volatile memory.

[Configuration of display device]
Next, the configuration of the display device 5 will be described.
The display device 5 displays an observation image of the specimen SP by the microscope 2 and various information of the microscope system 1 under the control of the control device 4. The display device 5 is configured using a display panel such as liquid crystal or organic EL (Electro Luminescence).

[Microscope system processing]
Next, processing executed by the microscope system 1 will be described. FIG. 3 is a flowchart showing an outline of processing executed by the microscope system 1. FIG. 4A to FIG. 4G are diagrams schematically showing a lighting location of the lighting unit 251 when the ring illumination unit 25 rotates. In the following, a case where the number of the lighting units 251 of the ring illumination unit 25 is 16 will be described. Further, the description will be made assuming that the minimum rotation resolution of the ring illumination unit 25 is 22.5 °, and lighting points are expressed by hatching.

  As shown in FIG. 3, first, the microscope control unit 28 determines whether or not the lighting portion of the ring illumination unit 25 has been changed based on an instruction signal input from the operation device 3 via the control device 4. (Step S101). Specifically, the microscope control unit 28 sets the current lighting part set value ptn of the ring illumination unit 25 based on the instruction signal input from the operation device 3 via the control device 4 to the previous ring illumination unit 25. It is determined whether or not the lighting part set value ptn_prev is the same (ptn = ptn_prev). When the microscope control unit 28 determines that the lighting part of the ring illumination unit 25 has been changed (step S101: Yes), the microscope system 1 proceeds to step S102 described later. On the other hand, when the microscope control unit 28 determines that the lighting part of the ring illumination unit 25 has not been changed (step S101: No), the microscope system 1 proceeds to step S105 described later.

  In step S <b> 102, the microscope control unit 28 changes the lighting part of the ring illumination unit 25 based on the instruction signal input from the controller device 3 via the control device 4.

  Subsequently, the microscope control unit 28 updates the previous value of the lighting part of the ring illumination unit 25 (step S103). Specifically, the microscope control unit 28 changes the previous lighting part setting value ptn_prev of the ring illumination unit 25 based on the instruction signal input from the operation device 3 via the control device 4. The lighting part set value ptn of the unit 25 is updated (ptn = ptn_prev).

  Thereafter, the microscope control unit 28 clears the current stage rotation angle and the accumulated stage angle (step S104). Specifically, the microscope control unit 28 sets the current stage rotation angle angle to 0 (angle = 0 °), and sets the cumulative stage rotation angle angle_add to 0 (angle_add = 0 °).

  Subsequently, the microscope control unit 28 determines whether or not the function of linking the stage 20 and the ring illumination unit 25 is effective (step S105). Specifically, the microscope control unit 28 determines whether or not the flag of the function that makes the stage 20 and the ring illumination unit 25 continuous is on. When the microscope control unit 28 determines that the function of linking the stage 20 and the ring illumination unit 25 is effective (step S105: Yes), the microscope system 1 proceeds to step S106 described later. On the other hand, when the microscope control unit 28 determines that the function of interlocking the stage 20 and the ring illumination unit 25 is not effective (step S105: No), the microscope system 1 proceeds to step S115 described later.

  In step S106, the microscope control unit 28 determines whether or not the stage 20 has been rotated. Specifically, the microscope control unit 28 determines whether or not an instruction signal for instructing rotation of the stage 20 is input from the operation device 3 via the control device 4. When the microscope control unit 28 determines that the stage 20 has been rotated (step S106: Yes), the microscope system 1 proceeds to step S107 described later. On the other hand, when the microscope control unit 28 determines that the stage 20 is not rotating (step S106: No), the microscope system 1 returns to step S101 described above.

  In step S107, the microscope control unit 28 updates the accumulated stage rotation angle. Specifically, the microscope control unit 28 adds the current stage rotation angle angle to the accumulated stage rotation angle angle_add (angle_add = angle_add + angle).

  Subsequently, the microscope control unit 28 clears the current stage rotation angle (step S108). Specifically, the microscope control unit 28 clears the current stage rotation angle angle (angle = 0).

  Thereafter, the microscope control unit 28 calculates the rotation angle of the ring illumination unit 25 (step S109). Specifically, the microscope control unit 28 divides the cumulative stage rotation angle angle_add by a constant ANGLE (22.5 °) to thereby determine the rotation amount shift_n of the ring illumination unit 25 of the ring illumination unit 25. Calculate the rotation angle. More specifically, as shown in FIG. 4A, when the stage 20 rotates 50 ° in the clockwise direction (CW direction) (angle = 50 °), the microscope control unit 28 determines the cumulative stage rotation angle angle_add (50 °). ) Is divided by a constant ANGLE (22.5 °) to calculate the rotation amount shift_n of the ring illumination unit 25 (50 ° / 22.5 ° = 2).

  Subsequently, the microscope control unit 28 determines whether or not the ring illumination unit 25 needs to be rotated (step S110). Specifically, the microscope control unit 28 determines that the ring illumination unit 25 needs to be rotated when the rotation amount shift_n calculated in step S109 described above is 1 or more. When the microscope control unit 28 determines that the ring illumination unit 25 needs to be rotated (step S110: Yes), the microscope system 1 proceeds to step S111 described later. On the other hand, when the microscope control unit 28 determines that the rotation of the ring illumination unit 25 is not necessary (step S110: No), the microscope system 1 returns to step S101 described above.

  In step S111, the microscope control unit 28 updates the current value of the lighting part of the ring illumination unit 25. Specifically, the microscope control unit 28 sets the current lighting region setting value ptn of the ring illumination unit 25 as a return value by using the value given by the argument pattern as the value shifted by the number given by shift_number. Update to

  Subsequently, the microscope control unit 28 changes the lighting part of the ring illumination unit 25 (step S112). Specifically, the microscope control unit 28 sets the lighting part Ringled (pattern) of the ring illumination unit 25 to a value given by the argument pattern.

  Thereafter, the microscope control unit 28 updates the previous value of the lighting part of the ring illumination unit 25 (step S113). Specifically, the microscope control unit 28 updates the previous lighting part setting value ptn_prev of the lighting part of the ring illumination to the current lighting part value ptn of the ring illumination part 25 (ptn_prev = ptn).

  Subsequently, the microscope control unit 28 updates the accumulated stage rotation angle (step S114). Specifically, the microscope control unit 28 subtracts a value obtained by multiplying the cumulative stage rotation angle angle_add by the constant ANGLE and the rotation amount shift_n of the ring illumination unit 25 (angle_add = angle_add−ANGLE × shift_n), thereby accumulating. Update the stage rotation angle. More specifically, as shown in FIG. 4B, the microscope control unit 28 subtracts a value obtained by multiplying the cumulative stage rotation angle angle_add by the constant ANGLE and the rotation amount shift_n of the ring illumination unit 25 (angle_add = 50 ° − (−22.5 ° × 2) = 5 °).

  Thereafter, when an instruction signal for ending the observation of the specimen SP is input from the operation device 3 (step S115: Yes), the microscope system 1 ends this process.

  On the other hand, when the instruction signal for ending the observation of the specimen SP is not input from the operation device 3 (Step S115: No), the microscope system 1 returns to Step S101 described above. Under the situation where Steps S101 to S115 are repeated, as shown in FIG. 4C, the microscope control unit 28 causes the stage 20 to rotate counterclockwise (CWW direction) by 15 ° (angle = −15 °). ), The rotation amount shift_n of the ring illumination unit 25 is calculated by dividing the cumulative stage rotation angle angle_add (5 ° −15 ° = −10 °) by the constant ANGLE (22.5 °) (−10). ° / 22.5 ° = 0). Therefore, as shown in FIG. 4D, the microscope control unit 28 does not change the lighting part of the ring illumination unit 25 and updates only the cumulative stage rotation angle (angle_add = −10 °).

  Thereafter, as shown in FIG. 4E, when the stage 20 is rotated 30 ° counterclockwise (CWW direction), ANGLE (with respect to the accumulated stage rotation angle angle_add (−10 ° −30 ° = −40 °)) The rotation amount shift_n of the ring illumination unit 25 is calculated by dividing by 22.5 ° (−40 ° / 22.5 ° = −1). For this reason, as shown in FIG. 4F, the microscope control unit 28 moves one lighting part of the ring illumination unit 25 counterclockwise and updates the accumulated stage rotation angle (angle_add = −40 ° − (22 .5 ° × −1) = − 17.5).

  Subsequently, when an instruction signal for changing the lighting pattern of the ring illumination unit 25 is input from the operation device 3, the microscope control unit 28 changes the lighting pattern of the ring illumination unit 25 as illustrated in FIG. Each of the stage rotation angle angle and the cumulative stage rotation angle angle_add is cleared (angle = 0 °, angle_add = 0). As a result, as shown in FIGS. 5A and 5B, the lighting position and illumination direction of the ring illumination unit 25 rotate following the rotation of the stage 20, thereby preventing the shadow direction of the sample SP from changing. Can do.

  According to the first embodiment of the present invention described above, even when the stage 20 is rotated, the lighting position of the ring illumination unit 25 rotates following the rotation, so that the shadow direction of the sample SP changes. Can be prevented.

(Embodiment 2)
Next, a second embodiment of the present invention will be described. The microscope system according to the second embodiment has a configuration different from that of the microscope 2 of the microscope system 1 according to the first embodiment described above, and a process to be executed is different. In the following, after describing the configuration of the microscope system according to the second embodiment, the processing executed by the microscope system will be described. In addition, the same code | symbol is attached | subjected to the structure same as the microscope system 1 which concerns on Embodiment 1 mentioned above, and description is abbreviate | omitted.

[Configuration of microscope system]
FIG. 6 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 2 of the present invention. A microscope system 1a according to FIG. 6 includes a microscope 2a instead of the microscope 2 of the microscope system 1 according to the first embodiment described above.

[Configuration of microscope]
The microscope 2a includes a stage 20a and a detection unit 21a instead of the stage 20 and the drive unit 21 of the microscope 2 according to the first embodiment described above.

  A specimen SP is placed on the stage 20a. The stage 20 a has a disk shape and is provided in the microscope main body 24 so as to be rotatable about the optical axis of the objective lens 23. The stage 20a is rotated by the user.

  The detection unit 21a detects the rotation angle of the stage 20a. FIG. 7 is a top view schematically showing the configuration of the detection unit 21a. As shown in FIG. 7, the detection unit 21 a has a disk shape, and slits 211 a in which slits are formed at predetermined intervals along the circumferential direction, and light that has passed through the slits of the slits 211 a is detected. And an encoder head 212a for detecting the rotation amount of the stage 20a. The encoder head 212 a is configured using a light emitting diode, a lens, a fixed slit, a phototransistor, a comparison circuit, and the like, and outputs the rotation angle of the stage 20 a to the microscope control unit 28. In addition, although the detection part 21a was demonstrated using the transmission type encoder, you may use a reflection type encoder, for example.

[Microscope system processing]
Next, processing executed by the microscope system 1a will be described. FIG. 8 is a flowchart showing an outline of processing executed by the microscope system 1a.

  In FIG. 8, Steps S201 to S203 correspond to Steps S101 to S103 in FIG.

  In step S204, the microscope control unit 28 clears the accumulated stage rotation angle, the number of input pulses from the detection unit 21a, and the number of accumulated pulses. Specifically, the microscope control unit 28 sets the cumulative stage rotation angle angle_add to 0 (angle_add = 0), the input pulse number pulse from the detection unit 21a to 0 (pulse = 0), and the cumulative pulse number pulse_add to 0 ( It is cleared to pulse_add = 0).

  Subsequently, the microscope control unit 28 determines whether or not the function of linking the stage 20a and the ring illumination unit 25 is effective (step S205). When the microscope control unit 28 determines that the function of linking the stage 20a and the ring illumination unit 25 is effective (step S205: Yes), the microscope system 1a proceeds to step S206 described later. On the other hand, when the microscope control unit 28 determines that the function of linking the stage 20a and the ring illumination unit 25 is not effective (step S205: No), the microscope system 1a proceeds to step S221 described later.

  In step S206, the microscope control unit 28 adds the number of input pulses from the detection unit 21a to the cumulative number of pulses. Specifically, the microscope control unit 28 adds the input pulse number pulse from the detection unit 21a to the accumulated pulse number pulse_add (pulse_add = pulse_add + pulse).

  Subsequently, the microscope control unit 28 clears the number of input pulses from the detection unit 21a (step S207). Specifically, the microscope control unit 28 clears the number of pulses input from the detection unit 21a (pulse = 0).

  Thereafter, the microscope control unit 28 updates the timer (step S208). Specifically, the timer t is incremented (t = t + 1).

  Subsequently, the microscope control unit 28 determines whether or not a certain time has elapsed (step S209). Specifically, the microscope control unit 28 determines whether or not a certain time (for example, 10 sec) has elapsed. When the microscope control unit 28 determines that a certain time has elapsed (step S209: Yes), the microscope system 1a proceeds to step S210 to be described later. On the other hand, when the microscope control unit 28 determines that the predetermined time has not elapsed (step S209: No), the microscope system 1a returns to step S201 described above.

  In step S210, the microscope control unit 28 resets the timer. Specifically, the microscope control unit 28 resets the timer t to 0 (t = 0).

  Subsequently, the microscope control unit 28 converts the cumulative number of pulses into a rotation angle (step S211). For example, the microscope control unit 28 converts 1 pulse as 1 ° (angle = pulseToDegee (pulse_add)).

  Thereafter, the microscope control unit 28 clears the cumulative number of pulses (step S212). Specifically, the microscope control unit 28 clears the cumulative pulse number pulse_add (pulse_add = 0).

  Steps S213 to S221 correspond to steps S106 to S115 of FIG.

  According to the second embodiment of the present invention described above, even when the stage 20a is rotated, the microscope control unit 28 rotates the lighting position of the ring illumination unit 25 based on the detection result of the detection unit 21a. Therefore, it is possible to prevent the shadow direction of the sample SP from changing.

  Further, according to the second embodiment of the present invention, since the stage 20a can be manually rotated, an inexpensive apparatus can be provided.

  In the second embodiment, the detection unit 21a may detect the rotation angle of the stage 20a at a predetermined cycle.

(Embodiment 3)
Next, a third embodiment of the present invention will be described. The microscope system according to the third embodiment has a configuration different from that of the microscope 2 of the microscope system 1 according to the first embodiment described above, and a process to be executed is different. In the following, after describing the configuration of the microscope system according to the third embodiment, the processing executed by the microscope system will be described. In addition, the same code | symbol is attached | subjected to the structure same as the microscope system 1 which concerns on Embodiment 1 mentioned above, and description is abbreviate | omitted.

[Configuration of microscope system]
FIG. 9 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 3 of the present invention. A microscope system 1b shown in FIG. 9 includes a microscope 2b instead of the microscope 2 of the microscope system 1 according to the first embodiment described above.

[Configuration of microscope]
The microscope 2b includes a stage 20b and a detection unit 21b instead of the stage 20 and the drive unit 21 of the microscope 2 according to the first embodiment described above.

  A specimen SP is placed on the stage 20b. The stage 20b has a disk shape and is provided in the microscope main body 24 so as to be rotatable about the optical axis of the objective lens 23. The stage 20b is rotated by the user.

  The detector 21b detects the rotation angle of the stage 20b. FIG. 10 is a top view schematically showing the configuration of the detection unit 21b. As shown in FIG. 10, the detection unit 21 b has a disk shape, and a scale unit 211 b in which a scale is formed at a predetermined interval along the circumferential direction, and by detecting light transmitted through the scale of the scale unit 211 b. And a scale head 212b for detecting the rotation amount of the stage 20b. The scale head 212b is configured using a light emitting diode, a lens, a phototransistor, a comparison circuit, and the like, and outputs the rotation angle of the stage 20b to the microscope control unit 28. In addition, although the detection part 21b was demonstrated using the transmissive | pervious scale, you may use a reflective scale, for example.

[Microscope system processing]
Next, processing executed by the microscope system 1b will be described. FIG. 11 is a flowchart showing an outline of processing executed by the microscope system 1b.

  In FIG. 11, steps S301 to S303 correspond to the above-described steps S101 to S103 in FIG.

  In step S304, the microscope control unit 28 clears the accumulated stage rotation angle and sets the previous scale value to be the same as the current scale value. Specifically, the microscope control unit 28 sets the cumulative stage rotation angle angle_add to 0 (angle_add = 0) and sets the previous scale value scale_prev to be the same as the current scale value scale (scale_prev = scale). .

  Steps S305 to S308 correspond to steps S207 to S210 in FIG.

  In step S309, the microscope control unit 28 determines whether the current scale value detected by the detection unit 21b is different from the previous scale value. When the microscope control unit 28 determines that the current scale value detected by the detection unit 21b is different from the previous scale value (step S309: Yes), the microscope system 1b proceeds to step S310 described later. On the other hand, when the microscope control unit 28 determines that the current scale value detected by the detection unit 21b is not different from the previous scale value (step S309: No), the microscope system 1b proceeds to the above-described step S301. Return.

  In step S310, the microscope control unit 28 calculates the difference between the current scale value detected by the detection unit 21b and the previous scale value. Specifically, the microscope control unit 28 calculates the difference scale_def by subtracting the current scale value scale detected by the detection unit 21b from the previous scale value scale_prev.

  Subsequently, the microscope control unit 28 updates the previous scale value (step S311). Specifically, the microscope control unit 28 updates the previous scale value scale_prev to the current scale value scale (scale_prev = scale).

  Thereafter, the microscope control unit 28 converts the difference scale value into a rotation angle (step S312). For example, when the distance from the center position of the stage 20b to the scale element is 10 cm, the microscope control unit 28 sets the distance k between one scale when the stage 20b is rotated to 0.174 cm (k = [{ 10 [cm] / cos (1 °)} ^ 2-10 [cm] ^ 2] ^ (1/2)). That is, the microscope control unit 28 calculates the scale value [cm] /0.174 [cm] = 1 ° as the stage rotation amount. Therefore, the microscope control unit 28 converts the difference scale value [cm] into an angle by dividing by 0.174.

  Steps S313 to S322 correspond to the above-described steps S106 to S115 in FIG.

  According to the third embodiment of the present invention described above, even when the stage 20b is rotated, the microscope control unit 28 rotates the lighting position of the ring illumination unit 25 based on the detection result of the detection unit 21b. Therefore, it is possible to prevent the shadow direction of the sample SP from changing.

  In the second embodiment of the present invention, the stage 20b can be manually rotated, so that an inexpensive apparatus can be provided.

(Embodiment 4)
Next, a fourth embodiment of the present invention will be described. The microscope system according to the fourth embodiment has a configuration different from that of the microscope 2 of the microscope system 1 according to the first embodiment described above, and a process to be executed is different. In the following, after describing the configuration of the microscope system according to the fourth embodiment, the processing executed by the microscope system will be described. In addition, the same code | symbol is attached | subjected to the structure same as the microscope system 1 which concerns on Embodiment 1 mentioned above, and description is abbreviate | omitted.

[Configuration of microscope system]
FIG. 12 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 4 of the present invention. A microscope system 1c shown in FIG. 12 includes a microscope 2c instead of the microscope 2 of the microscope system 1 according to the first embodiment described above.

[Configuration of microscope]
The microscope 2c includes a recording unit 27c instead of the recording unit 27 of the microscope 2 according to the first embodiment described above.

  The recording unit 27c records various information related to the microscope 2c and various programs executed by the microscope 2c. The recording unit 27c is configured using a nonvolatile memory, a volatile memory, or the like. The recording unit 27c includes a ring illumination rotation amount information recording unit 271c.

  The ring illumination rotation amount information recording unit 271c records ring illumination rotation amount information in which the rotation amount of the stage 20 and the rotation amount of the ring illumination unit 25 are associated with each other.

  FIG. 13 is a diagram showing an outline of the ring illumination rotation amount information table T1 indicating the ring illumination rotation amount information recorded by the ring illumination rotation amount information recording unit 271. In FIG. 13, the clockwise rotation of the stage 20 is positive (+) and the counterclockwise rotation is negative (−).

  In the ring illumination rotation amount information table T1 shown in FIG. 13, the stage rotation amount of the stage 20 and the ring illumination rotation amount of the ring illumination unit 25 are recorded in association with each other. For example, when the stage rotation amount of the stage 20 is 90.0 ° -112.4 °, the ring illumination rotation amount of the ring illumination unit 25 (the movement amount of the lighting unit 251) is described as four.

[Microscope system processing]
Next, processing executed by the microscope system 1c will be described. FIG. 14 is a flowchart illustrating an outline of processing executed by the microscope system 1c.

  In FIG. 14, steps S401 to S408 correspond to the above-described steps S101 to S108 of FIG.

  In step S409, the microscope control unit 28 calculates the movement amount of the lighting unit 251 of the ring illumination unit 25 based on the ring illumination rotation amount information recorded by the ring illumination rotation amount information recording unit 271c. Specifically, the microscope control unit 28 corresponds to an operation amount between 45.0 ° and 67.4 ° of the ring illumination rotation amount information table T1 when the rotation amount of the stage 20 moves 50 ° clockwise. Therefore, the amount of movement of the ring illumination unit 25 is calculated so that the two lighting units 251 of the ring illumination unit 25 are moved clockwise (shift_n = shift_tbl [angle_add]). After step S409, the microscope system 1c proceeds to step S410.

  Steps S410 to S415 correspond to the above-described steps S110 to S115 of FIG.

  According to the fourth embodiment of the present invention described above, even when the stage 20 is rotated, the microscope control unit 28 rotates the lighting position of the ring illumination unit 25 based on the ring illumination rotation amount information. Therefore, it is possible to prevent the shadow direction of the sample SP from changing.

(Embodiment 5)
Next, a fifth embodiment of the present invention will be described. The microscope system according to the fifth embodiment has a configuration different from that of the microscope 2 of the microscope system 1 according to the first embodiment described above and a process to be executed. In the following, after the configuration of the microscope system according to the fifth embodiment is described, processing executed by the microscope system will be described. In addition, the same code | symbol is attached | subjected to the structure same as the microscope system 1 which concerns on Embodiment 1 mentioned above, and description is abbreviate | omitted.

[Configuration of microscope system]
FIG. 15 is a schematic diagram showing a schematic configuration of a microscope system according to Embodiment 5 of the present invention. A microscope system 1d shown in FIG. 15 includes a microscope 2d instead of the microscope 2 of the microscope system 1 according to the first embodiment described above.

[Configuration of microscope]
The microscope 2d includes a recording unit 27d instead of the recording unit 27 of the microscope 2 according to the first embodiment described above.

  The recording unit 27d records various information related to the microscope 2d and various programs executed by the microscope 2d. The recording unit 27d is configured using a nonvolatile memory, a volatile memory, or the like. The recording unit 27d includes a ring illumination rotation amount information recording unit 271d.

  The ring illumination rotation amount information recording unit 271d includes presence / absence information regarding the rotation amount of the stage 20, the rotation amount of the ring illumination unit 25, and the presence or absence of lighting of the lighting unit 251 adjacent to the rotation direction of the lighting unit 251 of the ring illumination unit 25. And the ring illumination rotation amount information in association with each other.

  FIG. 16 is a diagram showing an outline of ring illumination rotation amount information recorded by the ring illumination rotation amount information recording unit 271d. In FIG. 16, the clockwise rotation of the stage 20 is positive (+) and the counterclockwise rotation is negative (−).

  The ring illumination rotation amount information table T2 shown in FIG. 16 includes the stage rotation amount of the stage 20, the ring illumination rotation amount of the ring illumination unit 25, and the lighting unit 251 adjacent to the rotation direction of the lighting unit 251 of the ring illumination unit 25. Presence / absence information regarding the presence / absence of lighting is recorded in association with each other. For example, when the stage rotation amount of the stage 20 is “90.00 °” − “101.24 °”, the ring illumination rotation amount of the ring illumination unit 25 (movement amount of the lighting unit 251) is described as “4”. In addition, it is described that the lighting of the adjacent lighting unit 251 is “not performed”. On the other hand, when the stage rotation amount of the stage 20 is “101.25 °” − “112.49 °”, the ring illumination rotation amount of the ring illumination unit 25 is described as “4”, and the adjacent lighting unit 251 It is described as “ON”.

  Here, the reason for lighting the lighting part 251 adjacent to the rotation direction of the lighting part 251 of the ring illumination part 25 is demonstrated. FIG. 17 is a top view schematically showing the brightness and darkness that occurs when the sample SP is illuminated by the ring illumination unit 25. The sample SP is arranged at the center of the stage 20, and the ring illumination unit 25. It is a figure which shows the state in which the 16 lighting parts 251 are formed. Further, FIG. 17 shows a state in which the sample SP is irradiated with light from the lighting unit 251 (1) and the lighting unit 251 (2).

  As shown in FIG. 17, when the illumination light is irradiated from the lighting unit 251-1 and the lighting unit 251- (2) to the specimen SP, the irradiation region of the lighting unit 251-1 and the irradiation of the lighting unit 251-2. The region D1 where the region overlaps each other is shaded, while the regions D2 and D3 where the irradiation region of the lighting unit 251-1 and the irradiation region of the lighting unit 251-2 do not overlap each other are canceled by one light. Shadows may not occur. For this reason, in this Embodiment 5, when rotating the lighting part 251 of the ring illumination part 25 according to the rotation amount of the stage 20, the lighting part 251 of the ring illumination part 25 is previously set according to the rotation amount of the stage 20. The presence or absence of lighting of the lighting unit 251 adjacent to the rotated rotation direction is related and recorded in the ring illumination rotation amount information table T2. Thereby, the interlocking direction of the ring illumination part 25 with respect to the stage 20 can be prescribed | regulated more finely.

[Microscope system processing]
Next, processing executed by the microscope system 1d will be described. FIG. 18 is a flowchart illustrating an outline of processing executed by the microscope system 1d. FIG. 19A to FIG. 19G are diagrams schematically illustrating a lighting location of the lighting unit 251 when the ring illumination unit 25 rotates.

  18, step S501 to step S508 correspond to step S101 to step S108 in FIG. 3 described above.

  In step S509, the microscope control unit 28 calculates the amount of movement of the lighting unit 251 of the ring illumination unit 25 based on the ring illumination rotation amount information recorded by the ring illumination rotation amount information recording unit 271d. Specifically, the microscope control unit 28 corresponds to the operation amount between 45.00 ° and 56.24 ° of the ring illumination rotation amount information table T2 when the rotation amount of the stage 20 moves 50 ° clockwise. Therefore, the amount of movement of the ring illumination unit 25 is calculated so that the two lighting units 251 of the ring illumination unit 25 are moved clockwise (shift_n = shift_tbl [angle_add]). On the other hand, since the microscope control unit 28 corresponds to the operation amount between 56.25 ° and 65.49 ° of the ring illumination rotation amount information table T2 when the rotation amount of the stage 20 moves 60 ° clockwise, The amount of movement of the ring illumination unit 25 is calculated so that the two illumination units 251 of the ring illumination unit 25 are moved in the clockwise direction, and adjacent to the previous illumination unit 251 shifted by two illumination units 251 The amount of movement of the ring illumination unit 25 is calculated so that the lighting unit 251 to be turned on is also lit.

  Steps S510 to S515 correspond to the above-described steps S109 to S114 of FIG.

  In step S516, the microscope control unit 28 determines whether it is necessary to light one lighting unit 251 of the ring illumination unit 25 in the rotation direction of the stage 20. When the microscope control unit 28 determines that one lighting unit 251 of the ring illumination unit 25 in the rotation direction of the stage 20 needs to be turned on (step S516: Yes), the microscope system 1d proceeds to step S517 described later. . On the other hand, when the microscope control unit 28 determines that it is not necessary to light one lighting unit 251 of the ring illumination unit 25 in the rotation direction of the stage 20 (step S516: No), the microscope system 1d will be described later. The process proceeds to step S521.

  In step S517, the microscope control unit 28 determines whether or not the rotation direction of the stage 20 is clockwise. When the microscope control unit 28 determines that the rotation direction of the stage 20 is clockwise (step S517: Yes), the microscope system 1d proceeds to step S518 described later. On the other hand, when the microscope control unit 28 determines that the rotation direction of the stage 20 is not clockwise (step S517: No), the microscope system 1d proceeds to step S519 described later.

  In step S518, the microscope control unit 28 sets one clockwise last light source in the ring illumination unit 25 to additional lighting. After step S518, the microscope system 1d proceeds to step S520 described later.

  In step S519, the microscope control unit 28 sets one last light source around the counterclockwise direction in the ring illumination unit 25 to additional lighting. After step S519, the microscope system 1d proceeds to step S520 described later.

  Subsequently, the microscope control unit 28 changes the lighting unit 251 of the ring illumination unit 25 (step S520), and clears the current stage rotation angle (step S521).

  Thereafter, when an instruction signal for ending the observation of the specimen SP is input from the operation device 3 (step S522: Yes), the microscope system 1d ends this process.

  On the other hand, when the instruction signal for ending the observation of the specimen SP is not input from the operation device 3 (step S522: No), the microscope system 1d returns to step S501 described above. Under such a situation where Steps S501 to S522 are repeated, as shown in FIG. 19A, when the stage 20 is rotated 50 ° clockwise (angle = 50 °), the microscope control unit 28 The cumulative stage rotation angle angle_add is updated (angle_add = 50 °), and the rotation amount shift_n of the ring illumination unit 25 is calculated as two based on the ring illumination rotation amount information table T2 (shift_n = shift_tbl [50 °] = 2) As shown in FIG. 19B, the microscope control unit 28 subtracts a value obtained by multiplying the cumulative stage rotation angle angle_add by the constant ANGLE and the rotation amount shift_n of the ring illumination unit 25 (angle_add = 50 ° − (− 22). 5 ° × 2) = 5 °).

  After that, as shown in FIG. 19C, when rotated 20 ° counterclockwise (CCW direction) (angle = −20 °), the microscope control unit 28 sets the cumulative stage rotation angle angle_add (5 ° −20 ° = −15 °), and based on this calculation result and the ring illumination rotation amount information table T2, the rotation amount shift_n of the ring illumination unit 25 is calculated as 0 (shift_n = shift_tbl [−15 °] = 0). At this time, as shown in FIG. 19D, the microscope control unit 28 lights one lighting unit 251 in the rotation direction of the ring illumination unit 25 based on the presence / absence information in the ring illumination rotation amount information table T2 (add_tbl [− 15 °]), the current stage rotation angle angle is cleared (angle = 0 °), and the cumulative stage rotation angle angle_add is updated (angle_add = −15 °).

  Subsequently, as shown in FIG. 19E, when the stage 20 is rotated 30 ° counterclockwise (CCW direction) (angle = 30 °), the cumulative stage rotation angle angle_add is updated (angle_add = −15 °). Based on the ring illumination rotation amount information table T2, the rotation amount shift_n of the ring illumination unit 25 is calculated as two (shift_n = shift_tbl [−45 °] = 2).

  Thereafter, as shown in FIG. 19F, the microscope control unit 28 rotates the lighting unit 251 of the ring illumination unit 25 by two in the counterclockwise direction (CCW direction). At this time, the lighting unit 251 in the rotation direction of the ring illumination unit 25 is not lit based on the presence / absence information in the ring illumination rotation amount information table T2. The microscope control unit 28 then clears the current stage rotation angle angle (angle = 0 °) and subtracts a value obtained by multiplying the cumulative stage rotation angle angle_add by the constant ANGLE and the rotation amount shift_n of the ring illumination unit 25. (Angle_add = −45 ° − (− 22.5 ° × 2) = 0 °).

  Subsequently, when an instruction signal for changing the lighting pattern of the ring illumination unit 25 is input from the operation device 3, the microscope control unit 28 changes the lighting pattern of the ring illumination unit 25 as illustrated in FIG. 19G. Each of the stage rotation angle angle and the cumulative stage rotation angle angle_add is cleared (angle = 0 °, angle_add = 0).

  According to the fifth embodiment of the present invention described above, even when the stage 20 is rotated, the microscope control unit 28 turns on the ring illumination unit 25 based on the ring illumination rotation amount information table T2. And the lighting direction of the lighting unit 251 adjacent to the rotation direction is controlled, so that the interlocking direction of the ring illumination unit 25 with respect to the stage 20 can be more finely defined, so that the shadow direction of the sample SP changes. Can be prevented.

(Other embodiments)
In the present invention, a microscope system including a microscope, a control device, an operation device, and a display device has been described as an example. For example, an objective lens that magnifies a specimen, an imaging function that images a specimen via the objective lens, and an image display The present invention can also be applied to an imaging device having a display function to be performed, such as a video microscope.

  Further, in the present invention, the ring illumination unit 25 is changed in the lighting position of the plurality of lighting units, so that the lighting unit of the ring illumination unit 25 is rotated following the rotation of the stage. A mechanical shutter may be provided in each of these, and the lighting unit of the ring illumination unit 25 may be rotated by following the rotation of the stage according to the opening / closing operation of the shutter.

  In the description of the flowchart in the present specification, the processing context between steps is clearly indicated using expressions such as “first”, “after”, “follow”, “and”, etc. The order of the processes necessary for implementation is not uniquely determined by their representation. That is, the order of processing in the flowcharts described in this specification can be changed within a consistent range.

  In addition, this invention is not limited to Embodiment 1-5, A various invention can be formed by combining suitably the some component currently disclosed by each embodiment and modification. For example, some constituent elements may be excluded from all the constituent elements shown in each embodiment or modification, or may be formed by appropriately combining the constituent elements shown in different embodiments or modifications. May be.

DESCRIPTION OF SYMBOLS 1,1a-1d Microscope system 2,2a-2d Microscope 3 Operation apparatus 4 Control apparatus 5 Display apparatus 20,20a, 20b Stage 21 Drive part 21a, 21b Detection part 22 Revolver 23 Objective lens 24 Microscope main body part 25 Ring illumination part 26 Eyepiece 27, 27c, 27d Recording unit 28 Microscope control unit 100 Rotation stage 211a Slit unit 211b Scale unit 212a Encoder head 212b Scale head 251 Lighting unit 271, 271c, 271d Ring illumination rotation amount information recording unit SP sample T1, T2 ring Illumination rotation amount information table

Claims (8)

An objective lens;
A stage that is rotatable about the optical axis of the objective lens and on which a specimen is placed;
A ring illumination unit that has a plurality of lighting units arranged in an annular shape around the objective lens, and emits illumination light for illuminating the specimen;
An eyepiece for observing an observation image of the specimen collected by the objective lens;
An input unit that receives an input of a lighting signal that indicates a lighting position of the plurality of lighting units;
Based on the lighting signal input from the input unit, a microscope control unit for lighting the plurality of lighting units corresponding to the lighting position,
With
The microscope control unit rotates the lighting positions of the plurality of lighting units following the rotation of the stage when the stage rotates. A drive unit for rotating the stage;
The input unit can accept an input of a rotation signal that indicates the amount of rotation of the stage;
When the rotation signal is input from the input unit, the microscope control unit rotates the stage to the driving unit so as to be the rotation amount, and based on the rotation amount, the plurality of lighting units The microscope according to claim 1, wherein the lighting position is rotated. A detector for detecting the amount of rotation of the stage;
The microscope according to claim 1, wherein the microscope control unit moves lighting positions of the plurality of lighting units according to the rotation amount detected by the detection unit.   4. The detection unit according to claim 3, wherein the detection unit is one of an encoder that detects the rotation amount of the stage, converts it into a pulse number, and outputs it, and a scale that detects and outputs the rotation angle of the stage. Microscope. A drive unit for rotating the stage;
The input unit can accept an input of a rotation signal that indicates the amount of rotation of the stage;
5. The microscope according to claim 3, wherein when the rotation signal is input from the input unit, the microscope control unit causes the driving unit to rotate the stage so that the rotation amount is obtained. A drive unit for rotating the stage;
A recording unit for recording ring illumination rotation amount information in which the rotation amount of the ring illumination unit corresponding to the rotation amount of the stage is associated;
Further comprising
The input unit can accept an input of a rotation signal that indicates the amount of rotation of the stage;
When the rotation signal is input from the input unit, the microscope control unit causes the driving unit to rotate the stage so as to obtain the rotation amount, and based on the rotation amount and the ring illumination rotation amount information. The microscope according to claim 1, wherein lighting positions of the plurality of lighting units are rotated.   The microscope according to claim 6, wherein the ring illumination rotation amount information includes presence / absence information related to presence / absence of lighting of a lighting unit adjacent in a rotation direction of the plurality of lighting units. The input unit is capable of accepting an input of an invalid signal that invalidates a function of rotating the plurality of lighting units following the rotation of the stage or an valid signal that is valid,
The microscope according to any one of claims 1 to 7, wherein the microscope control unit switches the function according to the invalid signal or the valid signal.

Images