JP2018177734A - Fat absorption promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a fat absorption promoter that can be safely and conveniently ingested and has an excellent fat absorption promoting action.SOLUTION: A fat absorption promoter according to the present invention contains oil and fat, which contain β-palmitic acid in high concentrations, as an active ingredient. The fat absorption promoter is excellent in fat digestion and absorption, and is suitable for e.g. supplementing infants' diet or improving infants' nutrition state.SELECTED DRAWING: None

Description

  The present invention relates to a fat absorption enhancer.

  Fatty acid, which is a component of fat, is one of the main energy sources for humans, and is particularly important for infants and children. Fatty acids are combined with triglycerides to constitute fats, and after ingestion, they are hydrolyzed by pancreatic lipase in the human body, and fatty acids bound to α-position and α′-position of triglycerides are absorbed as free fatty acids. At this time, there is a possibility that the absorption of free fatty acid may differ depending on the type of fatty acid and the binding site to triglyceride, etc., and various studies have been made so far.

  For example, in JP-A-2002-180082 (Patent Document 1), fats and oils in which ω-3 fatty acids such as docosahexaenoic acid and eicosapentaenoic acid are bonded at the 2 position and medium-chain fatty acids are bonded at the 1 and 3 positions are It is described that the said fats and oils have a good absorption factor of omega-3 fatty acid. In addition, JP-2006-521368 (patent document 2) describes a lipid improving agent comprising a triglyceride in which a polyunsaturated fatty acid is bound to the 2-position of the triglyceride.

JP, 2002-180082, A Japanese Patent Application Publication No. 2006-521368

  As mentioned above, various substances for promoting absorption of fats and fatty acids are being searched. However, substances known so far have not always had sufficient effect. Therefore, it is an object of the present invention to provide a fat absorption promoter which can be safely and easily ingested, and on the other hand, a sufficient fat absorption promoting effect can be obtained.

  In order to solve the above problems, the present inventors focused on palmitic acid among fatty acids, particularly focusing on palmitic acid (hereinafter also referred to as β-palmitic acid) binding to β-position of glycerides. , I examined the characteristics again in detail. And as a result, it discovered that the fats and oils which contain the (beta) position-palmitic acid in high concentration had the outstanding absorption promotion effect of fat, and completed the present invention.

That is, the present invention is as follows.
[1] A fat absorption promoter comprising, as an active ingredient, a fat and oil containing β-palmitic acid.
[2] The fat absorption improving agent according to [1], wherein the ratio of β-palmitic acid to total palmitic acid of fats and oils is 54% or more and less than 98%.
[3] Use of a fat and oil containing β-palmitic acid for producing a composition that promotes absorption of fat.
[4] The use according to [3], wherein the proportion of β-palmitic acid in total palmitic acid of fats and oils is 54% or more and less than 98%.

The present invention also includes the following inventions.
[5] A method for promoting fat absorption, which comprises using an oil / fat containing β-palmitic acid.
[6] The method for promoting absorption of fat according to [5], wherein the ratio of β-palmitic acid to total palmitic acid of fat and oil is 54% or more and less than 98%.
[7] A fat and oil containing β-palmitic acid for use in the production of a fat absorption enhancer.
[8] The oil and fat as described in [7], wherein the ratio of β-palmitic acid to total palmitic acid of oil and fat is 54% or more and less than 98%.
[9] A food for infants and infants, which comprises a fat absorption promoter containing as the active ingredient a fat and oil containing β-palmitic acid.
[10] The infant food according to [9], wherein the ratio of β-palmitic acid to total palmitic acid of fat and oil is 54% or more and less than 98%.
[11] A prepared milk powder comprising a fat absorption promoter containing a fat and oil containing β-palmitic acid as an active ingredient.
[12] The prepared powdered milk according to [11], wherein the ratio of β-palmitic acid to total palmitic acid of fat and oil is 54% or more and less than 98%.

  ADVANTAGE OF THE INVENTION According to this invention, the fat absorption promoter which has the outstanding effect can be provided. The active ingredient β-palmitic acid of the present invention has a long eating experience and its safety is well supported. In addition, the composition containing the fat absorption promoter of the present invention is excellent in the digestive and absorbability of fat, and is very useful, for example, for nutritional support and improvement of the nutritional status of infants and the elderly.

In animal experiments in which the influence of differences in the β-position binding ratio of palmitic acid on the absorption rate of palmitic acid and total lipids, the amount of excretion of palmitic acid in feces in Comparative Example 1, Comparative Example 2, and Example 1 Is a graph showing the difference between Each group was run at n = 8 and the results are shown as mean values. Vertical bars indicate standard deviations. There is a significant difference between the different signs (P <0.05). In animal experiments in which the influence of differences in the β-position binding ratio of palmitic acid on the absorption rate of palmitic acid and total lipids, the amount of excretion of total lipids in feces in Comparative Example 1, Comparative Example 2, and Example 1 Is a graph showing the difference between Each group was run at n = 8 and the results are shown as mean values. Vertical bars indicate standard deviations. There is a significant difference between the different signs (P <0.05).

  The present invention is a fat absorption promoter comprising, as an active ingredient, a fat or oil containing β-palmitic acid. The β-position-palmitic acid constituting the active ingredient of the present invention is a substance in which palmitic acid is bonded to the β-position of glyceride, and refers to all the same compounds in chemical formula. Also, β-palmitic acid may be pure or a mixture with other substances. For example, β-palmitic acid may be measured by a known measurement method, and the raw material whose presence has been confirmed may be used as it is. As a rich raw material (mixture) of β-palmitic acid, representative is lard, which can also be used. Also, for example, a commercially available product of β-palmitic acid may be purchased and appropriately blended with other raw materials.

  In the fat and oil containing β-palmitic acid which is the active ingredient of the present invention, the ratio of β-palmitic acid in the total palmitic acid of the fat and oil (the binding ratio of palmitic acid to β-position) is 65% or more and 75% The following are preferable, 67% or more and 73% or less are more preferable, and 68% or more and 72% or less are especially preferable. If the ratio is less than 65%, the effect as the fat absorption promoter of the present invention can not be sufficiently obtained. Moreover, when the said ratio exceeds 75%, it can not manufacture stably enough.

  The fat absorption enhancer of the present invention exerts its function by being orally ingested by humans and other mammals. In the present invention, intake by eating and drinking is preferable.

  The present invention is also the use of a fat or oil containing β-palmitic acid for producing a composition that promotes absorption of fat. The present invention is also a method for promoting fat absorption, characterized by using a fat and oil containing β-palmitic acid. Furthermore, the present invention is also a fat and oil containing β-palmitic acid for use in the production of a fat absorption enhancer.

  The present invention may be taken alone or in combination with a normal diet. The fat absorption enhancer of the present invention is particularly suitable for nutritional supplementation and improvement of the nutritional condition of infants and the like, because it is particularly excellent in digestive and absorption properties of fat as compared with the conventional composition containing fat and oil. Here, infants include infants and infants, and more specifically include infants, infants and newborns, and more specifically include infants, infants, newborns, premature infants, premature infants and low birth weight infants. The term "infant" refers to a child who is in infancy, and the term "infancy" refers to a period in which milk or other milk is used as a main nutrient source, and in the case of humans, usually less than 1 year old corresponds to infancy. The term "infant" generally refers to a child who is in preschool age. The term "neonate" refers to a child in the neonatal period, and the term "neonatal period" means a period immediately after birth, and in the case of humans, usually within 4 weeks after birth corresponds to the neonatal period.

  In the present invention, for example, a fat and oil containing β-palmitic acid can be combined with other raw materials and the like to be used as a composition or supplement capable of promoting absorption of fat, infant formula milk powder, and the like. In this case, as described above, since the present invention is particularly suitable for feeding and improving the nutritional status of infants and infants, the use as formulated milk powder for infants is particularly preferable.

  The dose of the fat absorption enhancer of the present invention can be appropriately set in consideration of various factors such as the nutritional condition, age, weight and symptoms of the user. The dose of the fat absorption enhancer of the present invention is not particularly limited. Further, the weight per unit package of the fat absorption promoter of the present invention is not particularly limited, but for example, the weight is preferably in the range of 10 g to 500 g, and preferably in the range of 25 g to 250 g. More preferably, it is most preferably in the range of 50 g to 200 g. Further, the above unit packaging may be not only unit packaging per bag, box, container, unit packaging per unit contained in them, or unit packaging per day. A plurality of days, for example, an amount obtained by packaging a suitable quantity for intake for one week may be packaged, or a plurality of individual packages may be included.

  Hereinafter, the present invention will be more specifically described based on examples. Note that this embodiment does not limit the present invention.

Example 1
The influence of the difference in the β-position binding ratio of palmitic acid on palmitic acid absorption rate was examined. The group which ingests the fats and oils, the moderate fats and oils, and the high fats and oils in which the β-position binding ratio of palmitic acid is low was set as Comparative Example 1, Comparative Example 2, and Example 1, respectively. The composition of the feed consumed by each group is shown in Table 1. Further, by adjusting the composition of the “sample fat and oil” shown in Table 1, the β-position binding ratio of palmitic acid in the samples consumed by each group was adjusted. The composition of main fatty acids and the β-position binding ratio of palmitic acid in each group are shown in Table 2.

  A 5-week-old SD rat was acclimated for 1 week, then divided into 3 groups of 8 animals (Comparative Example 1, Comparative Example 2, Example 1), and a diet containing fats and oils differing only in the β-position binding ratio of palmitic acid ( Table 1 and Table 2) were given for one week. The feces for the last 3 days of the study were collected and subjected to lipid analysis. There were no significant differences between the groups in the food intake, body weight gain and fecal dry weight of the rats during the experiment (Table 3, shown as mean ± standard deviation).

  The collected feces were freeze-dried and then crushed and subjected to lipid extraction by the method of Jeejeebhoy et al. (Clin. Biochem., 1970). The extracted lipid was subjected to fatty acid analysis by GC method and total lipid amount analysis by gravimetric method. As a result, the amount of palmitic acid excreted in feces was significantly reduced in Example 1 as compared with Comparative Example 1 and Comparative Example 2 although there was no significant difference in the amount of fatty acid ingested (FIG. 1). In addition, the total amount of fecal lipids was also significantly reduced in Example 1 (FIG. 2). From these results, the absorbability of palmitic acid and total lipid changes depending on the position of binding to triglyceride, and the larger the ratio of binding to β-position, the smaller the amount of palmitic acid and total lipid excreted, It has become clear that palmitic acid and total lipid absorbability is improved.

Example 2
The following experiment was conducted for the purpose of simulating the characteristic at the time of digestion after intake of oil and fat. The fats and oils of Example 2 and Comparative Example 3 were prepared. The compositions of the main fatty acids of Example 2 and Comparative Example 3 are shown in Table 4. The major difference between the fats and oils of the example and the comparative example is only the binding ratio of palmitic acid to the β-position, and the other major fatty acid compositions are almost the same. An artificial digestion reaction liquid was prepared with the composition shown in Table 5 using the fats and oils of these Example 2 and Comparative Example 3. The concentrations of the components in the artificial digestion reaction solution after preparation were 20 mg / mL of bile acid, 2.4 mg / mL of lipase, 19 mM of calcium chloride, 150 mM of sodium chloride, and 2.5% of fat and oil. The lipase used was "Lipase from porcine pancreas (Type II)" (Sigma Aldrich, Cat. No. L3126).

  About 40 mL of the artificial digestion reaction solution described above is placed in a 50 mL glass beaker for each of Example 2 and Comparative Example 3 and reacted for 2 hours while stirring with a magnetic stirrer in a water bath set at 37 ° C. The During the reaction, using an automatic titrator, 0.5 N sodium hydroxide solution was added dropwise so that the pH was always 7.0. Before and after the reaction, the particle size and zeta potential of the reaction solution were measured. The particle size of the reaction solution was measured by a laser diffraction / scattering particle size distribution measuring apparatus (manufactured by Beckman Coulter, LS13320). The zeta potential was measured by a zeta potential measurement system (ELS-Z1 manufactured by Otsuka Electronics Co., Ltd.).

  The measurement results are shown in Table 6. In both Example 2 and Comparative Example 3, the particle size of the artificial digestion reaction solution after the digestion reaction increased. However, the value of the particle size itself was smaller in Example 2, and it was considered that Example 2 had better absorbability from this. The absolute value of the zeta potential increased after the digestion reaction in both Example 2 and Comparative Example 3. However, the increase amount in Example 2 was smaller, and the absolute value itself was also smaller in Example 2 than in Comparative Example 3. The zeta potential is an index of surface charge of particles, and as the absolute value is larger, particles repel each other, so it can be said that they are in a stable state without aggregation. In other words, in the oil of the comparative example having a large absolute value of zeta potential, the particles are in a stable state, so dissociation of fatty acid or monoglyceride from the particles hardly occurs on the surface of small intestinal epithelial cells, and the absolute value of zeta potential is small Since the oil of Example 3 is in the unstable state of the particles, it was considered that the dissociation of the fatty acid or monoglyceride from the particles was likely to occur on the surface of the small intestinal epithelial cells, and the state was easy to be absorbed.

  From the above results, fats and oils containing high concentrations of palmitic acid with a high binding ratio to the β position are superior in fat digestibility compared to fats and oils containing a low concentration of palmitic acid having a high binding ratio to the β position. It turned out that Therefore, a composition using an oil and fat containing palmitic acid in a high concentration at a high binding ratio to the β position is superior to the composition containing a conventional oil and fat in the ability to digest and absorb fat, for example, infants and elderly people It is considered to be very useful for nutritional support and nutritional condition improvement.

Claims (4)

  A fat absorption promoter comprising as an active ingredient a fat and oil containing β-palmitic acid.   The fat absorption improving agent according to claim 1, wherein the proportion of β-palmitic acid in total palmitic acid of fats and oils is 54% or more and less than 98%.   Use of a fat or oil containing β-palmitic acid for producing a composition that promotes absorption of fat. The use according to claim 3, wherein the proportion of β-palmitic acid in total palmitic acid of fats and oils is 54% or more and less than 98%.