JP2017015501A - Stirring device, stirring system, platelet aggregation evaluation device, and stirring method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an agitation device capable of agitating a liquid in a container using a simple configuration.SOLUTION: A cell 20 with an agitator 56 comprising a stick-like magnetic material provided in an inner space 44 thereof is set on a rotary table 28 to be rotated. A plurality of magnets 54 are arranged on an inner peripheral surface of a ring-like magnet holding member 52 along a peripheral direction, the magnet holding member being disposed on an outer peripheral side of the rotary table 28. Magnetic force intermittently acts on the agitator 56, which causes the agitator 56 in the cell to rotate, agitating a liquid in the cell thereby.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a stirrer that stirs a liquid, a stirrer system, a platelet aggregating ability evaluation apparatus that evaluates platelet aggregating ability, and a stirring method.

In experiments and analyses, when a substance in a liquid is dissolved or when two or more different kinds of liquids are mixed, the liquid is agitated to make the liquid in the container uniform. As a device for stirring the liquid in the container, for example, a stirring device that stirs a magnetic stirrer with a magnetic stirrer that generates a magnetic field for rotating the stirrer is known. In addition, a magnetic unit comprising a plurality of permanent magnets in which a stirrer having a magnetic pole (a bar-shaped magnet) is placed in a container and the container side is alternately arranged with S-pole permanent magnets and N-pole permanent magnets in one direction. A stirrer that rotates a stirrer in a container by moving the side of the container in one direction is known (Patent Document 1).
Liquid stirring devices and stirring methods are used in various fields, for example, for examination of platelet aggregation ability. As a method for evaluating platelet aggregation ability, a method developed by Born et al. In 1962 for detecting optical changes while stirring platelet-rich plasma (PRP) is still widely used (Non-patent Document 1). . In this method, platelet-rich plasma (PRP) is separated by centrifugation from blood to which sodium citrate is added as an anticoagulant, and while stirring the platelet-rich plasma (PRP), a platelet aggregation inducer called an agonist is added, This is a method of recording the degree of subsequent aggregation by optical change. As a stirring method for this platelet-rich plasma (PRP), a method using a magnetic stirrer and a stirrer is generally used, and a platelet aggregating capacity measuring device using the stirring method is known (Patent Document 2).

Japanese Patent Application Laid-Open No. 2010-066606. Japanese Patent Application Laid-Open No. 2002-082118.

Nature, vol.194, pp927-929 (1962)

  In Patent Document 1, a stirrer having a magnetic pole (a rod-shaped magnet) is placed in a container, and the container side includes a plurality of permanent magnets in which S-pole permanent magnets and N-pole permanent magnets are alternately arranged in one direction. The stirrer is rotated in the container by moving the magnetic unit in one direction along the side of the container.

  However, a plurality of permanent magnets must be connected so that the S poles and the N poles are alternately arranged in one direction, the number of permanent magnets is large, and the manufacture of the magnetic unit is complicated. Further, unless the S pole and the N pole are alternately arranged in one direction, it is difficult to smoothly rotate the stirring bar.

  In view of the above facts, an object of the present invention is to provide a stirrer, a stirrer system, a platelet aggregating ability evaluation apparatus, and a stirrer that have a simple configuration for uniformly stirring a liquid in a container.

  The stirrer according to claim 1, wherein the stirrer moves relative to the container so that the bar-like magnetic body placed in the container is separated from the side surface of the container or the extension line of the side surface in a horizontal direction. There is a magnetism generating means for intermittently applying a magnetic force to the magnetic body to rotate the magnetic body.

  In the stirrer according to claim 1, the magnetism generating means moves relative to the container, so that the bar-shaped magnetic body placed in the container is separated from the side surface of the container or the horizontal line from the extension line of the side surface. A magnetic force can be intermittently applied to the magnetic material. When the magnetism generating means approaches and a magnetic force is applied, the rod-shaped magnetic body placed in the container is rotated so that one end side near the magnetism generating means faces the magnetism generating means. In addition, the magnetic body rotates by inertia even when the magnetism generating means is separated and no magnetic force is applied. When the magnetic generation means approaches again and a magnetic force is applied to the magnetic body, the magnetic body is rotated so that one end side closer to the magnetic generation means faces the magnetic generation means. In this way, by intermittently applying a magnetic force to the magnetic body, the magnetic body can be continuously rotated in the container, and the liquid in the container can be stirred by the rotating rod-shaped magnetic body. . Thereby, it is not necessary to continuously apply a magnetic force to the magnetic body, and the liquid in the container can be stirred by a simple device. In addition, since the magnetic material has one end side closer to the magnetism generating means regardless of which of the S pole and the N pole of the magnetism generating means is relatively moved, the magnetism generating means has the S pole and the N pole. Any of the poles may be moved relative to the container.

  According to a second aspect of the present invention, in the stirrer according to the first aspect, the magnetism generating means is a plurality of magnets arranged at intervals in one direction.

  In the stirrer according to claim 2, the magnetism generating means is a plurality of magnets arranged at intervals in one direction, and the container and the plurality of magnets move relative to each other in one direction, thereby On the other hand, a magnetic force can be given intermittently.

  The stirrer according to claim 3 is provided with a rotating table in which a plurality of containers in which rod-shaped magnetic bodies are put are arranged and rotated, and provided on a side of the rotating table, the circumferential direction of the rotating table. And a plurality of magnets arranged at intervals.

  In the stirring device according to the third aspect, the plurality of containers in which the rod-shaped magnetic bodies are put are arranged on the circumference of the rotary table. Since a plurality of magnets arranged at intervals in the circumferential direction of the rotary table are arranged on the side of the rotary table, when the rotary table is rotated, a magnetic force is intermittently applied to the rod-shaped magnetic body. Can be given. When a magnetic force is applied, the rod-shaped magnetic body placed in the container is rotated so that one end side close to the magnet faces the magnet. Also, the magnetic body rotates with inertia even when no magnetic force is applied. When a magnetic force is applied again to the magnetic body, the magnetic body is rotated so that one end side closer to the magnet faces the magnet. In this way, by intermittently applying a magnetic force to the magnetic body, the magnetic body can be continuously rotated in the container, and the liquid in the container can be stirred by the rotating rod-shaped magnetic body. .

  The stirrer according to claim 4 is provided with a container holding member in which a plurality of containers in which rod-shaped magnetic bodies are put are arranged in a circumferential direction, and on the side of the container holding member, A rotating member that rotates in the circumferential direction and includes a plurality of magnets arranged at intervals along the circumferential direction.

  In the stirrer according to the fourth aspect, the plurality of containers in which the rod-shaped magnetic bodies are put are arranged in the circumferential direction of the container holding member. On the side of the container holding member, there is provided a rotating member provided with a plurality of magnets that rotate in the circumferential direction of the container holding member and are arranged at intervals along the circumferential direction. When the rotating member is rotated, a magnetic force can be intermittently applied to the rod-like magnetic body placed in the container. When a magnetic force is applied, the rod-shaped magnetic body placed in the container is rotated so that one end side close to the magnet faces the magnet. Also, the magnetic body rotates with inertia even when no magnetic force is applied. Then, by again applying a magnetic force to the magnetic body, the magnetic body is rotated so that one end side closer to the magnet faces the magnet. In this way, by intermittently applying a magnetic force to the magnetic body, the magnetic body can be continuously rotated in the container, and the liquid in the container can be stirred by the rotating rod-shaped magnetic body. .

  A stirring system according to a fifth aspect includes the stirring device according to any one of the first to fourth aspects, a container for storing a liquid, and a rod-shaped magnetic body that is placed in the container. Is done.

  In the stirring system according to the fifth aspect, the rod-shaped magnetic body placed in the container can be rotated by the stirring device according to any one of the first to fourth aspects. By using such a stirring system, the magnetic body can be continuously rotated in the container, and the liquid in the container can be stirred by the rotating rod-shaped magnetic body.

  The platelet aggregating ability evaluation apparatus according to claim 6 irradiates light to a liquid containing platelets being stirred by the stirring device according to any one of claims 1 to 4 and the magnetic substance placed in the container. A light amount measuring device for measuring the amount of transmitted light or the amount of scattered light.

Since the platelet aggregating ability evaluation apparatus according to claim 6 includes the stirring device according to any one of claims 1 to 4, a reagent is added to the liquid containing platelets in the container to form a magnetic substance. Can be stirred.
Evaluating platelet aggregation ability by measuring the amount of transmitted light or scattered light when the liquid is irradiated with light while stirring the liquid to which the reagent has been added. Can do.

  The stirring method according to claim 7, wherein the magnetism generating means is moved relative to the container so that the bar-like magnetic body placed in the container is separated horizontally from the side surface of the container or the extension line of the side surface. A magnetic force is intermittently applied to the magnetic body from the position, and the liquid in the container is stirred by rotating the magnetic body.

In the stirring method according to claim 7, the magnetism generating means moves relative to the container, so that the rod-shaped magnetic body placed in the container is separated from the side surface of the container or the horizontal line from the extension line of the side surface. A magnetic force can be intermittently applied to the magnetic material. When the magnetism generating means approaches and a magnetic force is applied, the rod-shaped magnetic body placed in the container is rotated so that one end side near the magnetism generating means faces the magnetism generating means. In addition, the magnetic body rotates by inertia even when the magnetism generating means is separated and no magnetic force is applied. When the magnetic generation means approaches again and a magnetic force is applied to the magnetic body, the magnetic body is rotated so that one end side closer to the magnetic generation means faces the magnetic generation means. In this way, by intermittently applying a magnetic force to the magnetic body, the magnetic body can be continuously rotated in the container, and the liquid in the container can be stirred by the rotating rod-shaped magnetic body. . In this case, there is no need to continuously apply a magnetic force to the magnetic material, and the liquid in the container can be stirred by a simple method.
In addition, since the magnetic material has one end side closer to the magnetism generating means regardless of which of the S pole and the N pole of the magnetism generating means is relatively moved, the magnetism generating means has the S pole and the N pole. Any of the poles may be moved relative to the container.

  As described above, according to the stirring device, stirring system, platelet aggregation evaluation device, and stirring method of the present invention, there is an excellent effect that the liquid in the container can be stirred with a simple configuration.

It is a top view of the analyzer provided with the stirring apparatus which concerns on the 1st Embodiment of this invention. It is a top view which shows the principal part of a stirring apparatus. (A) is a sectional view taken along line 3 (A) -3 (A) of the stirring device shown in FIG. 2, and (B) is a sectional view taken along line 3 (B) -3 (B) of the stirring device shown in FIG. is there. It is a side view of a cell and a magnet which show an example of positional relation with a cell and a magnet which made a magnetic pole horizontal. It is a side view of a cell and a magnet showing other examples of a positional relationship between a cell and a magnet having magnetic poles in the vertical direction. It is a side view of the cell and magnet which show another example of the positional relationship of a cell and the magnet made into a set of two. It is a side view of a cell and a magnet which shows an example of positional relation between a cell and a magnet which attached a yoke. (A)-(F) are top views which show the positional relationship of a cell, a stirrer, and a magnet. (A)-(C) are top views which show the positional relationship of a cell, a stirrer, and a magnet. It is a side view of the stirring apparatus which concerns on 2nd Embodiment. (A) is a side view of the stirring apparatus according to the third embodiment, and (B) is a plan view of the stirring apparatus according to the third embodiment shown in (A). (A) shows the change in the light transmittance of platelet-rich plasma (PRP) when using a conventional stirring device of the type in which a stirring bar is rotated in a container by rotating a magnet disposed below the cell. (B) is a graph which shows the change of the light transmittance of platelet-rich plasma (PRP) at the time of using the stirring apparatus of the 3rd Embodiment of this invention.

[First Embodiment]
The analyzer 10 according to the first embodiment of the present invention will be described with reference to FIGS.
[Overall configuration of analyzer]
As shown in FIG. 1, the analyzer 10 of this embodiment includes a cassette loading unit 16 for loading a cassette 14 containing a plurality of sample containers 12, a cell loading unit 22 for loading a plurality of cells 20 described later, and a cell 20 A cell transport device 24 for transporting a sample, a pipette (not shown) for collecting a sample (liquid) to be analyzed from the sample container 12 and injecting it into the cell 20, and a reagent nozzle (not shown) for injecting a reagent into the cell 20. A pipette device 26 provided, a turntable 28 capable of loading a plurality of cells 20 in the circumferential direction, a reagent liquid tank 34 storing a reagent, a stirring device 38 described later for stirring the sample and the reagent injected into the cell 20, An analysis unit 42 for analyzing the sample is provided.

  As shown in FIG. 2 and FIG. 3 (A), the cell 20 is formed of a transparent synthetic resin in a vertically elongated column shape, and has a circular cross section extending in the vertical direction for containing a sample and a reagent in the center. A cell interior 44 is formed. The bottom surface 44A of the cell interior 44 is circular in plan view, and is formed flat and horizontally.

A rod-like stirrer 56 for stirring the liquid put in the cell 20 is placed in the cell interior 44. The length of the stirring bar 56 is smaller than the inner diameter of the cell interior 44. For this reason, the stirring bar 56 arranged in parallel on the bottom surface 44 </ b> A of the cell interior 44 is rotatable in the horizontal direction on the bottom surface 44 </ b> A of the cell interior 44.
In addition, as a magnetic body in the present invention, a magnetic body that rotates intermittently by applying a magnetic force intermittently from a position separated in the horizontal direction from the side surface of the container or an extended line of the side surface is particularly useful. There is no limitation, and examples thereof include ferromagnetic materials and paramagnetic materials. Examples of the ferromagnetic material include iron, stainless steel, nickel, cobalt, and an alloy containing iron, and examples of the paramagnetic material include sodium, aluminum, chromium, and platinum. A stirrer 56 in the following embodiment is formed of stainless steel.

  In the rotary table 28, loading holes 29 for loading the cells 20 are formed at equal intervals along the circumferential direction. The rotary table 28 is rotated in the direction of arrow CW or in the direction opposite to the direction of arrow CW by a motor whose rotation is controlled by a control device (not shown).

(Agitator)
As shown in FIG. 2, a stirring device 38 is provided in the vicinity of the outer peripheral portion of the rotary table 28. The stirring device 38 includes a ring-shaped magnet holding member 52 on the outer peripheral side of the rotary table 28. As shown in FIGS. 2 and 3B, a plurality of magnets 54 are attached to the inner side surface of the magnet holding member 52 at equal intervals along the circumferential direction. The magnet 54 of this embodiment is a cubic neodymium magnet, but may have other shapes.

As shown in FIG. 4, the magnet 54 extends horizontally from the imaginary line (two-dot chain line in the drawing) EL extending downward from the outer peripheral edge of the lower bottom surface 20 </ b> A of the cell 20 in the horizontal direction (radial direction outside of the rotary table 28). It is arranged so that there is a gap.
When the magnetic poles (N pole and S pole) of the magnet 54 are oriented in the horizontal direction, the height position of the upper end 54U of the magnet 54 is the upper end of the stirrer 56 placed horizontally on the bottom surface 44A of the cell interior 44. It is preferable that it is below the horizontal line HL which passes. The dotted line shown in FIG. 4 shows an example of the magnetic force lines of the magnet 54.

  As shown in FIG. 5, the magnet 54 may be arranged with its magnetic poles facing up and down. Also in this case, the height position of the upper end 54U of the magnet 54 is below the horizontal line HL passing through the lower end of the stirrer 56 placed horizontally on the bottom surface 44A of the cell interior 44, and the stirrer 56 is not lifted and stirred. It is preferable to be in a position where it is possible.

  Moreover, as shown in FIG. 6, you may use the two magnets 54 piled up and down. In this case, it is preferable that the mating surface 55 of the two magnets 54 is below the horizontal line HL passing through the upper end of the stirrer 56 placed horizontally on the bottom surface 44A of the cell interior 44.

  Further, as shown in FIG. 7, a cap (yoke) 57 having an opening on the cell 20 side may be attached to a magnet 54 in which magnetic poles are arranged in the horizontal direction. In this case, the height position of the upper end 57U of the yoke 57 is preferably equal to or less than the horizontal line HL passing through the upper end of the stirring bar 56 placed horizontally on the bottom surface 44A of the cell interior 44.

(Analysis Department)
As shown in FIG. 2, the analysis unit 42 is disposed between the magnet 54 on one side and the magnet 54 on the other side in the circumferential direction. As shown in FIG. 2 and FIG. 3 (A), the light source 58 is disposed on one side (for example, the radially inner side of the outer periphery of the rotary table 28) across the passage path of the cell 20 in the analysis unit 42, The optical sensor 60 is disposed on the other side (for example, radially outside the outer periphery of the turntable 28), and is configured such that the light L emitted from the light source 58 passes through the cell 20 and enters the optical sensor 60. ing.

  The light source 58 and the optical sensor 60 are located above the bottom surface 44A of the cell interior 44 so that the stirrer 56 placed on the bottom surface 44A does not block the optical path from the light source 58 to the optical sensor 60. It has become.

(Function, effect)
Below, the outline of the analysis process of the sample by the analyzer 10 of this embodiment is demonstrated.
(1) In the analyzer 10 of this embodiment, the cell loading unit 22 is loaded with a plurality of cells 20 in advance. The cell 20 is previously provided with a stirrer 56. The cell 20 loaded in the cell loading unit 22 is transported to the loading hole 29 of the turntable 28 by the cell transport device 24 and loaded. In this embodiment, a cassette 14 containing a plurality of sample containers 12 is loaded in the cassette loading unit 16.
(2) In the analyzer 10 of the present embodiment that has been prepared as described above, first, the pipette of the pipette device 26 collects a predetermined amount of platelet poor plasma (PPP) from a predetermined sample container 12, and the collected sample is used as a turntable. It injects into the predetermined | prescribed cell 20 with which 28 was loaded.

(3) The rotating table 28 is rotated, and the cell 20 containing platelet poor plasma (PPP) is disposed between the light source 58 of the analysis unit 42 and the optical sensor 60, and the amount of light transmitted through the cell 20 by the optical sensor 60. Is measured, and the transmittance of platelet poor plasma (PPP) serving as a reference (for example, the transmittance here is 100%) is measured.

(4) Next, a predetermined amount of platelet-rich plasma (PRP) is dispensed into another cell 20, and the rotary table 28 is continuously rotated at a predetermined rotational speed. In the present embodiment, the rotary table 28 is rotated in the arrow CW direction. For example, in a state before rotating the rotary table 28 (that is, in a stationary state), as shown in FIG. 8A, the direction of the stirrer 56 placed in the cell interior 44 of the cell 20 is the vertical direction of the drawing, Assuming that the cell 20 is arranged diagonally to the upper left of the magnet 54A, the rotary table 28 rotates in the direction of the arrow CW, and the cell 20 comes close to the magnet 54A as shown in FIG. When the magnetic force of 54A acts, one end 56A of the stirrer 56 is directed to the magnet 54A and the stirrer 56 rotates in the direction of arrow A.

  When the rotary table 28 rotates in the direction of the arrow CW and the magnet 54A further approaches the cell 20 as shown in FIG. 8C, the stirrer 56 maintains the state where one end 56A is directed to the magnet 54A and stirs. The child 56 continues to rotate in the direction of arrow A.

  When the turntable 28 further rotates in the direction of arrow CW, as shown in FIG. 8D, the cell 20 moves away from the magnet 54A. However, while the magnetic force of the magnet 54A acts on the stirrer 56, the stirrer 56 Since one end 56A of the magnet is kept directed to the magnet 54A, the stirrer 56 continues to rotate in the direction of arrow A.

  As shown in FIG. 8E, when the turntable 28 further rotates in the direction of the arrow CW and the cell 20 is separated from the magnet 54A, the magnetic force of the magnet 54A does not reach the stirrer 56. The rotation can continue in the direction of arrow A.

  As shown in FIG. 8F, when the turntable 28 further rotates in the direction of arrow CW and the cell 20 approaches the magnet 54B arranged on the arrow CW direction side of the magnet 54A, the stirrer 56 is moved to the position of the magnet 54B. The magnetic force acts, the other end 56B of the stirrer 56 is directed to the magnet 54A, and the stirrer 56 rotates in the arrow A direction.

  When the rotary table 28 rotates in the direction of the arrow CW and the cell 20 further approaches the magnet 54B as shown in FIG. 9A, the stirrer 56 maintains the state where the other end 56B is directed to the magnet 54A. Therefore, the stirrer 56 continues to rotate in the direction of arrow A.

  When the turntable 28 further rotates in the direction of arrow CW, as shown in FIG. 9B, the cell 20 moves away from the magnet 54B, but while the magnetic force of the magnet 54B is acting on the stirrer 56, the stirrer 56 Since the other end 56 </ b> B is maintained in the state directed to the magnet 54 </ b> B, the stirrer 56 rotates in the arrow A direction.

  As shown in FIG. 9C, when the rotary table 28 further rotates in the direction of the arrow CW and the cell 20 is separated from the magnet 54B, the magnetic force of the magnet 54B does not reach the stirrer 56. The rotation can continue in the direction of arrow A.

  In this way, by rotating the rotary table 28 in the arrow CW direction and causing the magnetic force of the plurality of magnets 54 to intermittently act on the stirrer 56 in the cell, the stirrer 56 in the cell is continuously moved in the arrow A direction. The platelet-rich plasma (PRP) in the cell 20 can be agitated.

While the rotary table 28 is continuously rotated, the cell 20 passes between the light source 58 and the optical sensor 60 of the analysis unit 42 as shown in FIG. The amount of light L transmitted through the cell 20 by the optical sensor 60 when passing through the optical sensor 60 is measured, and the transmittance of platelet rich plasma (PRP) (compared to the platelet poor plasma (PPP) as a reference) is measured. Measured. That is, the transmittance of platelet-rich plasma (PRP) placed in the cell 20 is measured a plurality of times while the turntable 28 is rotating.
Since the stirrer 56 in the cell rotates while being placed on the bottom surface 44A of the cell interior 44, the light L emitted from the light source 58 passes above the stirrer 56 and reaches the optical sensor 60.

(5) When the transmittance is stabilized after a predetermined time has elapsed, the rotation of the turntable 28 is temporarily stopped, and a reagent (agonist: ADP solution as an example. ADP solution as an example) is added to the cell 20 containing platelet-rich plasma (PRP). As an agonist, collagen, ristocetin, epinephrine, etc. can be used in addition to ADP). After the reagent is added, the rotation rate is measured a plurality of times for a predetermined time while rotating the rotary table 28 and stirring the platelet-rich plasma (PRP) and the reagent with the stirrer 56. When a reagent is added to platelet-rich plasma (PRP), an agglutination reaction occurs and the permeability of platelet-rich plasma (PRP) in the cell 20 increases. A higher transmittance over time indicates that the agglutination reaction is in progress. Thus, in the analyzer 10 of this embodiment, platelet aggregation ability can be evaluated by measuring the transmittance | permeability several times with time, stirring. Also, standardization is facilitated by measuring a large number of samples.

  In the analyzer 10 of the present embodiment, the rotary table 28 is temporarily stopped (may be about several seconds), the empty cell 20 is loaded, the sample is dispensed, and the reagent is added, and the platelet aggregation ability of a plurality of samples is measured. Can be evaluated sequentially.

  In the analyzer 10 of the present embodiment, the liquid can be stirred by rotating the stirrer 56 having no magnetic force as described above, similarly to a stirrer using a stirrer that is a conventional bar magnet. The same measured value as when using a conventional stirring device can be obtained.

  In the stirring device 38 of the present embodiment, a plurality of magnets 54 are provided on the outer side of the rotating table 28 that rotates with the plurality of cells 20 arranged in the circumferential direction, with the circumferentially spaced intervals. It has the outstanding effect that the liquid of the some cell 20 can be stirred, suppressing a number.

  Further, in the stirring device 38 of this embodiment, the liquid can be stirred in a large number of cells 20 without increasing the number of parts by a simple method of increasing the number of loading holes 29 for loading the cells 20.

[Second Embodiment]
Next, a stirring device 38 according to a second embodiment of the present invention will be described with reference to FIG. In addition, the same code | symbol is attached | subjected to the same structure as 1st Embodiment, and the description is abbreviate | omitted.
In the first embodiment, the ring-shaped magnet holding member 52 is provided on the outer peripheral side of the turntable 28, and the magnet 54 is provided on the inner peripheral surface of the magnet holding member 52. However, as shown in FIG. In the stirring device 38 of the present embodiment, an annular magnet holding member 90 having a diameter smaller than that of the rotary table 28 is provided immediately below the rotary table 28, and a plurality of magnets 54 are arranged in the circumferential direction on the outer peripheral surface of the magnet holding member 90. Is provided.

  In the stirring device 38 of the present embodiment, the position (height etc.) relationship between the magnet 54 and the stirring bar 56 is the same as that of the first embodiment, and this embodiment is also the rotary table 28 as in the first embodiment. , The stirring element 56 in the cell 20 can be rotated to stir the liquid in the cell 20.

[Third Embodiment]
Next, a stirring device 38 according to a third embodiment of the present invention will be described with reference to FIG. In addition, the same code | symbol is attached | subjected to the same structure as 1st Embodiment, and the description is abbreviate | omitted.
As shown in FIG. 11, the stirring device 38 of the present embodiment includes a cell holding member 72 and a magnet rotation device 74 disposed on the side of the cell holding member 72.

  The cell holding member 72 is formed with a loading hole 70 for loading the cell 20. The magnet rotation device 74 includes a rotary drum 80 made of a 24-hedron member whose axis is oriented in the vertical direction, and a motor 82 that rotates the rotary drum 80. A plurality of magnets 54 are attached to the outer peripheral surface of the rotating drum 80 at equal intervals in the circumferential direction. The positional relationship (height etc.) between the magnet 54 and the stirrer 56 is the same as in the first embodiment.

  In the stirring device 38 of this embodiment, the cell 20 and the magnet 54 can be relatively moved by rotating the rotary drum 80, and the stirrer 56 in the cell 20 is rotated as in the first embodiment. Thus, the liquid in the cell 20 can be stirred.

[Other Embodiments]
Although one embodiment of the present invention has been described above, the present invention is not limited to the above, and other various modifications can be made without departing from the spirit of the present invention. It is.

  The magnet 54 only needs to be able to rotate the stirrer 56 placed on the bottom surface 44A of the cell interior 44 of the cell 20, and the magnetic pole of the magnet 54 closest to the cell 20 may be an N pole or an S pole. The magnetic pole of the magnet 54 closest to the cell 20 may not be set to either one of the N pole and the S pole, or may be set at random. This can be realized because the stirrer 56 of this embodiment does not have a magnetic force, that is, does not have a magnetic pole. If the stirrer 56 is a bar magnet, the stirrer 56 cannot be smoothly rotated if the direction of the magnetic pole of the magnet 54 is random.

  If the magnetic force, horizontal position, height, etc. of the magnet 54 are set so that the stirrer 56 mounted on the bottom surface 44A of the cell interior 44 can be rotated horizontally, the magnet 54 will be the same as that described in the above embodiment. Not exclusively.

  When the height position of the magnet 54 is increased, the end of the stirrer 56 may be lifted and the stirrer 56 may be inclined with respect to the bottom surface 44 </ b> A of the cell interior 44. In order to prevent the stirrer 56 from tilting, it is preferable to determine the height positions of the stirrer 56 and the magnet 54 as shown in FIGS. The shape of the magnet 54 is not particularly limited, and the magnet 54 is not limited to a permanent magnet but may be an electromagnet.

  In the above embodiment, in order to examine the aggregation ability, the cell 20 was irradiated with light and the light transmittance in platelet poor plasma (PPP) and platelet rich plasma (PRP) was measured, but the cell 20 was irradiated with light. Thus, the aggregation ability can also be measured by measuring the scattered light of platelet poor plasma (PPP) and platelet rich plasma (PRP).

  In the above embodiment, the stirring device 38 is used for stirring platelet-rich plasma (PRP), but the present invention is not limited to this, and the stirring device 38 can also be used for stirring liquids other than platelet-rich plasma (PRP). .

(Test example)
First, platelet rich plasma (PRP) and platelet poor plasma (PPP) were prepared by the following procedure.
(1) As an anticoagulant, 50 mL of blood was collected using a syringe containing 5 mL of 0.129 mol / L (0.38%) citric acid.
(2) Aliquot into 15 mL tubes and centrifuge with a centrifuge (100 × g, 15 minutes, 25 ° C.).
(3) Platelet-rich plasma (PRP) in the supernatant was carefully pipetted.
(4) The remaining lower layer (blood cell) portion was further centrifuged (1500 g, 15 minutes, 25 ° C.).
(5) Platelet-poor plasma (PPP) in the supernatant was collected with a pipette.
Next, the platelet aggregation ability was evaluated by the following procedure using the stirring device of the third embodiment described above.
(1) Eight magnets were attached at intervals of 45 degrees along the outer periphery of the rotating drum so that the north pole was directed to the cell. At this time, the height of the magnet was set such that the top surface of the magnet was the same or slightly lower than the bottom surface of the cell hole.
(2) 100 μL of platelet-poor plasma (PPP) was dispensed into the cell, the light transmittance at 555 nm was measured, and the transmittance was taken as 100%.
(3) One stir bar (diameter 1 mm, length 3.9 mm) and 100 μL of platelet-rich plasma (PRP) were dispensed into another cell, and the cell was set in a cell holder.
(4) The rotating drum was continuously rotated at a speed of 100 rpm, and the spectroscopic measurement (time scan) of the cell was started simultaneously with stirring.
(5) After about 20 seconds, when the transmittance was stable, 10 μL of ADP solution (20 μmol / L, final concentration) was added into the cell with a pipette.
(6) The time scan measurement was performed for 5 minutes as it was.
FIG. 12 (A) shows the platelet-rich plasma (PRP) in the case of using a conventional stirrer of the type in which a stirrer that is a bar magnet is rotated in a container by rotating a magnet disposed below the cell. A change in light transmittance (a graph line indicated by a dotted line) is shown, and FIG. 12B uses the stirring device of the example to which the present invention is applied (the stirring device of the third embodiment). The change in the light transmittance of the platelet-rich plasma (PRP) in this case (graph line shown by a solid line) is shown.
As shown in the graph of FIG. 12, the test results when using the stirrer of the embodiment to which the present invention is applied and the test results when using the conventional stirrer are substantially the same, and the test results Thus, it can be seen that the stirrer of the example to which the present invention is applied can stir platelet-rich plasma (PRP) as in the conventional stirrer, and can promote the platelet aggregation reaction.

10 Analyzer (platelet aggregation ability evaluation device)
20 cells (containers)
22 Pipette 28 Rotary table 38 Stirrer 42 Analysis unit (light quantity measuring device)
52 Magnet Holding Member 54 Magnet 54A Magnet 54B Magnet 56 Stir Bar (Magnetic Material)
72 Cell holding member (container holding member)
80 Rotating drum (Rotating member)

Claims (7)

  By moving relative to the container, a magnetic force is intermittently applied to the magnetic body from a position in the horizontal direction away from the side surface of the container or an extension line of the side surface with respect to the rod-shaped magnetic body placed in the container. A stirrer having a magnetic generating means for rotating the magnetic body.   The stirring device according to claim 1, wherein the magnetism generating means is a plurality of magnets arranged at intervals in one direction. A rotating table in which a plurality of containers in which rod-shaped magnetic bodies are placed are arranged on the circumference and rotates,
A plurality of magnets provided on a side of the turntable and arranged at intervals in a circumferential direction of the turntable;
A stirring device having A container holding member in which a plurality of containers containing rod-shaped magnetic bodies are arranged in the circumferential direction;
A rotating member provided on a side of the container holding member, rotated in a circumferential direction of the container holding member, and provided with a plurality of magnets arranged at intervals along the circumferential direction;
A stirring device having The stirrer according to any one of claims 1 to 4,
A container for liquid,
A rod-like magnetic body to be placed in the container;
A stirring system composed of a. The stirrer according to any one of claims 1 to 4,
A light amount measuring device for measuring the amount of transmitted light or the amount of scattered light when irradiating light to a liquid containing platelets being stirred with the magnetic substance placed in the container;
An apparatus for evaluating platelet aggregation ability.   By moving the magnetism generating means relative to the container, the rod-shaped magnetic body placed in the container is intermittently moved relative to the magnetic body from a position horizontally separated from the side surface of the container or on the extension line of the side surface. A stirring method of applying a magnetic force and rotating the magnetic material to stir the liquid in the container.